• cells
  • Cells were labeled with 4 μM C6NBD-GlcCer or C6-NBD-SM at 37°C for 30 min. (nih.gov)
  • Note that in order to visualize intracellular fluorescence in b and d, basolateral membrane-located lipid analogues were partly removed by a brief back exchange procedure (5-10 min, 4°C). Alternatively, cells incubated as in a and c were subsequently subjected to a back exchange procedure at 4°C for 30 min to completely remove the basolateral PM pool of lipid analogues. (nih.gov)
  • After back exchange, the cells were incubated in HBSS, supplemented with 30 mM sodiumdithionite at 4°C for 7 min. (nih.gov)
  • Figure
  • Diltiazem hydrochloride (DLZ), d-cis diltiazem, d-cis-3-acetyloxy-5-[2-(dimethylamino) ethyl]-2,3-dihydro-2-(4-methoxyphenyl)-1,5-benzothiazepin-4(5H)one)hydrochloride (Figure 1 ) are one of the widely used benzothiazepine Ca 2+ -blocking drugs (calcium channel blockers). (hindawi.com)