The enzyme was most active in the high-pH region, with a maximum at pH 8.0 to 9.5, and had a temperature optimum of 55 degrees C. Heat stability was observed up to 70 degrees C. Substrate specificity studies suggested that the enzyme is identical with the 4-aminobutyrate:2-ketoglutarate transaminase (EC 2.6.1.19). (asm.org)
The recombinant glutamate:oxalacetate transaminase protein was used in immobilized form together with 4-aminobutyrate:2-ketoglutarate transaminase (EC 2.6.1.19) from E. coli for the production of L-phosphinothricin [L-homoalanin-4-yl-(methyl)phosphinic acid], the active ingredient of the herbicide Basta (AgrEvo GmbH), from its nonchiral 2-keto acid precursor 2-oxo-4-[(hydroxy)(methyl)phosphinoyl]butyric acid (PPO). (asm.org)
Stereospecific production of the herbicide phosphinothricin (glufosinate): purification of aspartate transaminase from Bacillus stearothermophilus, cloning of the corresponding gene, aspC, and application in a coupled transaminase process. (asm.org)
The gene encoding a 4-hydroxybutyryl-Co A transferase has been isolated from bacteria and integrated into the genome of bacteria also expressing a polyhydroxyalkanoate synthase, to yield an improved production process for 4HB-containing polyhydroxyalkanoates using transgenic organisms, including both bacteria and plants. (patents.com)
The gene from the most promising candidate, C . violaceum ω -transaminase (CV-TA), was expressed in a strain lacking pyruvate decarboxylase activity, which thereby accumulate the co-substrate pyruvate during glucose assimilation. (biomedcentral.com)
Furthermore, higher reaction rates were achieved using a strain containing several copies of CV-TA gene, highlighting the necessity to also increase the intracellular transaminase level. (biomedcentral.com)
In mammals, GAD exists in two isoforms with molecular weights of 67 and 65 kDa (GAD 67 and GAD 65 ), which are encoded by two different genes on different chromosomes ( GAD1 and GAD2 genes, chromosomes 4 and 10 respectively). (wikipedia.org)
The transaminase has a molecular mass of 43 kilodaltons by sodium dodecyl sulfate-gel analysis and an isoelectric point of 4.35. (asm.org)
[4] It does so using molecular oxygen (O 2 ), as well as iron (Fe 2+ ) and tetrahydrobiopterin as cofactors . (ipfs.io)
Stereospecific production of the herbicide phosphinothricin (glufosinate) by transamination: isolation and characterization of a phosphinothricin-specific transaminase from Escherichia coli. (asm.org)
L-2,4-diaminobutyric acid, also known as Dbu or alpha,gamma-diaminobutyrate, belongs to the class of organic compounds known as l-alpha-amino acids. (hmdb.ca)
Furthermore astrocytes are indispensible for glutamatergic and γ-aminobutyrate-ergic synaptic transmission being the supplier of neurotransmitters precursor glutamine via an astrocytic/neuronal cycle. (springer.com)
Furthermore, the engineered yeast strain with highest transaminase activity was also shown to be operational as whole-cell catalyst for the production of ( S )-1-phenylethylamine via asymmetric transamination of acetophenone, albeit with very low conversion. (biomedcentral.com)
In lieu of chemical catalysis, the use of ω -transaminase ( ω -TA) (E.C. 2.6.1.18) has emerged as a competitive alternative for bio-catalysed production of chiral amines, either via asymmetric transamination of ketones or via kinetic resolution of racemic amines. (biomedcentral.com)