• Hirsutenone Directly Targets PI3K and ERK to Inhibit Adipogenesis in 3T3-L1 Preadipocytes. (oregonstate.edu)
  • Using Oil Red O staining, we observed that HST dose-dependently suppresses lipid accumulation during adipogenesis in 3T3-L1 preadipocytes, concomitant with a decrease in peroxisome proliferator-activated receptor-γ (PPARγ), CCAAT/enhancer-binding protein α (C/EBPα) and fatty acid synthase (FAS) protein expression. (oregonstate.edu)
  • A metabolite of daidzein, 6,7,4'-trihydroxyisoflavone, suppresses adipogenesis in 3T3-L1 preadipocytes via ATP-competitive inhibition of PI3K. (oregonstate.edu)
  • Our objective was to determine the effects of 6,7,4'-THIF on adipogenesis in 3T3-L1 preadipocytes and elucidate the mechanisms of action involved. (oregonstate.edu)
  • Our results suggest that 6,7,4'-THIF suppresses adipogenesis in 3T3-L1 preadipocytes by directly targeting PI3K. (oregonstate.edu)
  • The present research was designed to examine the impact of EA on adipogenesis in 3T3-L1 preadipocytes and underlying mechanism of action involved. (gplmedicine.org)
  • jujubarepresses adipogenesis in 3T3-L1 preadipocytes [16]. (euromedh2020.com)
  • The morphological differentiation of 3T3-L1 preadipocytes includes the loss of "stress fibers" and the appearance of microfilament like structures that encase, in a complex manner, the cytosolic lipid spheres that appear during differentiation. (silverchair.com)
  • Key findings: Among five flavonoids isolated from GFF, only apigenin potently inhibited the differentiation of 3T3-L1 cells. (kist.re.kr)
  • Apigenin-treated 3T3-L1 cells failed to undergo clonal expansion during the early phase of adipocyte differentiation. (kist.re.kr)
  • Significance: These findings suggest that apigenin can prevent 3T3-L1 preadipocyte differentiation by the inhibition of the mitotic clonal expansion and the adipogenesis related factors and upregulation of the expression of multiple C/EBPβ inhibitors. (kist.re.kr)
  • Alteration by 2,3,7,8-Tetrachlorodibenzo-p-dioxin of CCAAT/enhancer binding protein correlates with suppression of adipocyte differentiation in 3T3-L1 cells. (aspetjournals.org)
  • We began to examine the mechanism by which TCDD inhibits differentiation of the established preadipocyte cell line 3T3-L1. (aspetjournals.org)
  • Examination of the expression of several early marker genes of preadipocyte differentiation through Northern blot analysis and of differentiation-dependent mitosis showed that TCDD did not interfere with the earliest known responses of preadipocytes to inducers of differentiation. (aspetjournals.org)
  • These data indicate that suppression of differentiation in the 3T3-L1 preadipocyte cell line by TCDD occurs at a short but defined period, during the differentiation program, and involves altered regulation of C/EBP, including the inhibition of C/EBP alpha. (aspetjournals.org)
  • Adipocyte differentiation, known as adipogenesis also, is an activity where fibroblast-like preadipocytes become adult adipocytes [6]. (euromedh2020.com)
  • This study aimed to determine whether I3C or DIM could increase glucose uptake via enhanced insulin sensitivity in 3T3‐L1 adipocytes, as well as the mechanism involved. (researchgate.net)
  • 3,3′-diindolylmethane (DIM)-a natural compound produced from indole-3-carbinol, found in cruciferous vegetables-enhances glucose uptake by increasing the activation of the insulin signaling pathway in 3T3-L1 adipocytes. (researchgate.net)
  • MISSION ® pLKO.1-puro Non-Target shRNA Control Transduction Particles have been used to generate 3T3-L1 (pre-adipocytes) control cell lines. (sigmaaldrich.com)
  • 2016. 18O-Tracer Metabolomics Reveals Protein Turnover and CDP-Choline Cycle Activity in Differentiating 3T3-L1 Pre-Adipocytes. . (oregonstate.edu)
  • Another WCMC-Q team project titled 'Validation and Functional Characterisation of Novel Factors in Senescent 3T3-L1 Pre-adipocytes' won third prize. (qnrf.org)
  • As a proof-ofconcept test, we applied cyclic stretch loading to the adipocyte model, differentiated adipocytes from 3T3-L1 preadipocytes, and evaluated stretch-induced activation of key insulin signaling molecules. (unl.edu)
  • Here, we describe the cellular origin and physiological significance of micro LDs (mLDs) that emerge in the cytoplasm during active lipolysis, as well as the roles of key lipolytic proteins on mLDs in differentiated 3T3-L1 adipocytes. (indexindex.com)
  • In differentiating 3T3-L1 cells, lipid spheres, the endoplasmic reticulum (ER), microperoxisomes, and mitochondria form "constellations" that may reflect the interplay of lipid metabolizing enzymes in these organelles. (silverchair.com)
  • In addition, apigenin inhibited the DNA-binding activity of C/EBPβ in differentiating 3T3-L1 cells by down-regulating the 35 kDa isoform of C/EBPβ (liver-enriched activating protein) and up-regulating the expression of two different sets of C/EBP inhibitors: C/EBP homologous protein and the phospho-liver-enriched inhibitory protein isoform of C/EBPβ. (kist.re.kr)
  • We sought to assess whether the flavonoid-rich fraction of crude extracts from Daphne genkwa Siebold et Zuccarini (GFF) might inhibit adipogenesis of 3T3-L1 cells. (kist.re.kr)
  • We show that piceatannol inhibits adipogenesis of 3T3-L1 preadipocytes in a dose-dependent manner at noncytotoxic concentrations. (oregonstate.edu)
  • Main methods: Cell viability of 3T3-L1 preadipocytes was assessed by MTT assays, and lipid accumulation was measured by Oil Red O. Adipogenesis related factors were checked by Western blot analysis. (kist.re.kr)
  • To compare the abilities of chicoric acid and its metabolites in scavenging free radicals and their effects on the viability of 3T3-L1 preadipocytes, an in vitro study of the metabolism of chicoric acid in rat liver microsomes was performed using liquid tandem mass spectrometry (HPLC-MS/MS). The results indicated that caffeic acid and caftaric acid were the hepatic phase I metabolites of chicoric acid. (rsc.org)
  • Our results suggest that HST attenuates adipogenesis by directly targeting PI3K and ERK during MCE in 3T3-L1 preadipocytes, underscoring the potential therapeutic application of HST in preventing obesity. (oregonstate.edu)
  • Previous studies have found that DIM can improve type 2 diabetes by enhancing glucose uptake through the activation of insulin signaling in 3T3-L1 cells, and by lowering the plasma glucose levels in high-fat-diet-fed obese mice [13, 14]. (researchgate.net)
  • DIM, a major metabolite of indole-3-carbinol, which is naturally produced in broccoli and cabbage, enhances glucose uptake through the improvement of insulin sensitivity in 3T3-L1 cells [13] . (researchgate.net)
  • In the 3T3-L1 cells HuR is constitutively expressed and localized predominantly to the nucleus in the preadipocytes. (uncg.edu)
  • The researchers collected samples of indoor dust from 11 homes in North Carolina and tested extracts from the samples in a mouse pre-adipocyte cell model, 3T3-L1 cells, often used to test compounds for potential effects on the accumulation of triglycerides, a type of fat. (gardeningchannel.com)
  • 3T3-L1 cells are utilized extensively like a cell tradition model to review the molecular control of adipogenesis [5]. (euromedh2020.com)
  • Methods 3T3‐L1 preadipocytes were differentiated by using a mixture of adipogenic inducers, including a suboptimal concentration of insulin. (researchgate.net)
  • cocosWolf, offers insulin-sensitizing activity in ST13 preadipocytes [15] as well as the chloroform small fraction ofZ. (euromedh2020.com)
  • These three compounds had strong capacities for scavenging free radicals and had been demonstrated to increase intracellular ROS levels in 3T3-L1 preadipocytes, thereby reducing cell vitality. (rsc.org)
  • Matsushima, T. , Yoshimura, N. and Koseki, Y. (2015) Effect of Soy Bean Isoflavon on Lipid Accumulation in 3T3-L1 Adipocytes. (scirp.org)
  • 2007) studied the effects of capsaicin on the induction of apoptosis and inhibition of lipid accumulation in 3T3-L1 preadipocytes and adipocytes. (ift.org)
  • Results showed that capsaicin efficiently induced apoptosis and inhibited adipogenesis in the preadipocytes and adipocytes. (ift.org)
  • MISSION ® pLKO.1-puro Non-Target shRNA Control Transduction Particles have been used to generate 3T3-L1 (pre-adipocytes) control cell lines. (sigmaaldrich.com)
  • 2016. 18O-Tracer Metabolomics Reveals Protein Turnover and CDP-Choline Cycle Activity in Differentiating 3T3-L1 Pre-Adipocytes. . (oregonstate.edu)
  • in human adipose tissue and 3T3-L1 pre-adipocytes. (cdc.gov)
  • We have found that CTHRC1 suppresses the differentiation of preadipocytes (precursors of fat cells) to adipocytes (mature fat cells) (Figure 7). (mmcri.org)
  • Obesity is characterized at the cellular level by an increase in the number and size of adipocytes differentiated from preadipocytes. (auburn.edu)
  • 3T3-L1 adipocytes have been fundamental in metabolic disease research for 30 years. (zen-bio.com)
  • Preadipocytes can be cultured as growing precursor cells or differentiated into adipocytes using medium supplemented with adipogenic and lipogenic hormones. (zen-bio.com)
  • This instruction manual describes procedures t o induce human preadipocytes to differentiate into mature adipocytes as well as culturing methods for human preadipocytes and adipocytes. (zen-bio.com)
  • The process of differentiating preadipocytes to adipocytes has been patent protected by ZenBio under US patent number 6153432. (zen-bio.com)
  • They found that TBBPA decreases the expression of genes that prevent preadipocytes from becoming mature adipocytes and increases the expression of key early genes that eventually activate PPARgamma, which is a known regulator for adipogenesis. (nih.gov)
  • Ectopic expression of Wnt-1 in 3T3-L1 preadipocytes stabilizes beta-catenin, activates TCF-dependent gene transcription, and blocks adipogenesis. (nih.gov)
  • Here, we explored the functional relationship between LKB1 and the mammalian target of rapamycin (mTOR) in regulating adipogenesis in rats and preadipocytes. (cheric.org)
  • In addition, adipogenesis of murine 3T3-L1 and human Simpson-Golabi-Behmel Syndrome preadipocytes was dose- and time-dependently reduced by PM, as demonstrated by Oil Red O staining and reduced expression of adipogenic differentiation genes. (nih.gov)
  • We hypothesize that CLA suppresses adipogenesis by reducing cellular levels of arachidonic acid (AA), an unsaturated fatty acid whose metabolites activate peroxisome proliferator activated receptor gamma (PPAR-gamma) and preadipocyte differentiation. (nih.gov)
  • In this study, we have investigated the role of the farnesoid X receptor (FXR), a bile acid-activated nuclear receptor, in regulating adipogenesis in a preadipocyte cell line (3T3-L1 cells). (aspetjournals.org)
  • We hypothesized that exposing preadipocytes to TBBPA could influence adipogenesis via genes other than those in the PPARγ pathway due to its structural similarity to bisphenol A, which demonstrates varied endocrine disrupting activities. (nih.gov)
  • In particular, we show KCTD1 expression 3T3-L1 cells increases upon adipogenesis induction. (cnr.it)
  • Honda inhibit adipogenesis through the activation of the AMPK signaling pathway in 3T3-L1 preadipocytes. (ddtjournal.com)
  • Adipogenesis is the formation of fat cells from cells called preadipocytes, and lipogenesis is the formation of fat. (nih.gov)
  • Here we examined effects of BPA, BPS, and BPF in low concentrations on differentiation in murine 3T3-L1 preadipocytes. (nih.gov)
  • In support of this idea, we have recently demonstrated that the trans-10, cis-12, but not the cis-9, trans-11, isomer of CLA decreased the TG content of differentiating cultures of murine 3T3-L1 and human preadipocytes. (nih.gov)
  • Differentiation of 3T3-L1 murine fibroblasts into adipocyte-like cells was conducted in presence of IBMX, dexamethasone and insulin and demonstrated by Oil Red O staining of accumulated lipid droplets. (pensoft.net)
  • 3T3-L1 preadipocytes have been widely used as an in vitro model for studying adipocyte differentiation. (cnr.it)
  • A) To induce adipocyte differentiation, 3T3-L1 preadipocytes were grown in induction medium containing MDI, insulin, and fetal bovine serum (FBS) for 8 days. (jkomor.org)
  • In this paper we studied the effects of genistein and EGCG on differentiation to adipocyte and lipid accumulation in 3T3L1 preadipocytes. (scirp.org)
  • Furthermore, proliferating preadipocytes treated with TBBPA, only prior to differentiation, exhibited increased adipocyte number and lipid accumulation after 8 days in normal culture conditions. (nih.gov)
  • CREB activation induced by mitochondrial dysfunction triggers triglyceride accumulation in 3T3-L1 preadipocytes. (uclouvain.be)
  • Effects of different inducers on lipid accumulation in differentiating 3T3-L1 preadipocytes. (jkomor.org)
  • In this research, we investigated the impact of WHW on the ER stress response and the insulin signaling pathway in 3T3-L1 preadipocytes. (gplmedicine.org)
  • 6. Differentiation-induced gene expression in 3T3-L1 preadipocytes. (nih.gov)
  • The differentiation of a preadipocyte into a mature adipocyte is a highly regulated process that requires a scripted program of transcriptional events leading to changes in gene expression. (aspetjournals.org)
  • cAMP-dependent protein kinase induction of PPAR (gamma) coactivator-1alpha (PGC-1alpha) and uncoupling protein 1 (UCP1) expression is an essential step in the commitment of preadipocytes to the brown adipose tissue (BAT) lineage. (elsevierpure.com)
  • B) 3T3-L1 preadipocytes were induced to differentiate with an induction medium containing MDI, insulin, and FBS for 8 days. (jkomor.org)
  • 3T3L1 preadipocytes derived from mice skin fibroblast were obtained from American Tissue Culture Collection. (scirp.org)
  • Chromatin immunoprecipitation studies demonstrated that C/EBPß and CREB bound to the CRE region in HIB1B and 3T3-L1 cell lysates. (elsevierpure.com)
  • A curated dataset of publicly available ChIP-sequencing of transcription factors, chromatin remodelers and histone modifications in the 3T3-L1 pre-adipocyte cell line. (bioconductor.org)
  • We studied the molecular mechanisms responsible for differential expression of PGC-1alpha in HIB1B (BAT) and 3T3-L1 white adipose tissue (WAT) precursor cell lines. (elsevierpure.com)
  • The recent isolation of preadipocyte populations in vivo and their identification by specific markers may become crucial for manipulating these populations as they become potential targets of novel therapeutics in treating condition of metabolic dysfunctions such as obesity. (scirp.org)
  • Originally derived from Swiss mouse embryo tissue by Dr. Ho ward Green of Harvard Medical School, the 3T3-L1 system has been pivotal in advancing the underst anding of basic cellular mechanisms associated with diabetes, obesity and related disorders. (zen-bio.com)
  • These data demonstrate that differentiation of preadipocytes toward the BAT rather than the WAT phenotype is controlled in part by the action of C/EBPß on the CRE in PGC-1alpha proximal promoter. (elsevierpure.com)
  • Mouse-derived 3T3-L1 preadipocytes were induced to differentiate and continually treated with TBBPA for 8 days. (nih.gov)
  • Wnt-1 stimulates 3T3-L1 preadipocytes to secrete factors that increase PKB/Akt phosphorylation at levels comparable with treatment with 10% serum. (nih.gov)
  • Treatment of 3T3-L1 preadipocytes with Kctd1-specific siRNA inhibited the differentiation, as indicated by reduction of expression of the specific adipogenic markers C/ebpalpha, Ppargamma2, Glut4, and Adiponectin. (cnr.it)
  • 3T3-L1 preadipocytes are useful model for physiological, pharmacological and cell signaling studies. (pensoft.net)
  • We further constructed overexpressed LKB1 (OE) and silenced LKB1 (Si) 3T3-L1 preadipocytes monoclonal cell lines. (cheric.org)
  • The preadipocytes are isolated by centrifugal force after collagenase treatment. (zen-bio.com)