• 3T3-L1 cells have a fibroblast-like morphology, but, under appropriate conditions, the cells differentiate into an adipocyte-like phenotype, providing an exemplar model for white adipocytes. (wikipedia.org)
  • In 1971, Green and Kehinde established a subline of 3T3 cells, called 3T3-L1, which demonstrated a greater propensity for differentiation into adipocytes and a higher affinity for lipid deposition. (wikipedia.org)
  • 3T3-L1 cells are commonly utilized as an effective model for white adipocytes because of their similar fibroblast-like morphology, lipid accumulation, gene expression, and basal energy exchanges and transformations. (wikipedia.org)
  • Although, some literature suggests that 3T3-L1 adipocytes can possess certain phenotypic signatures similar to other adipocyte lineages aside from white adipocytes. (wikipedia.org)
  • This study aimed to determine whether I3C or DIM could increase glucose uptake via enhanced insulin sensitivity in 3T3‐L1 adipocytes, as well as the mechanism involved. (researchgate.net)
  • 3,3′-diindolylmethane (DIM)-a natural compound produced from indole-3-carbinol, found in cruciferous vegetables-enhances glucose uptake by increasing the activation of the insulin signaling pathway in 3T3-L1 adipocytes. (researchgate.net)
  • TLK16998 alone had no effect on IR signaling in mouse 3T3-L1 adipocytes but, at concentrations as low as 3.2 μmol/l, enhanced the effects of insulin on the phosphorylation of the IR β-subunit and IR substrate 1, and on the amount of phosphatidylinositol 3-kinase that coimmunoprecipitated with IRS-1. (diabetesjournals.org)
  • TLK16998 also increased the potency of insulin in stimulating 2-deoxy- d -glucose uptake in 3T3-L1 adipocytes, with a detectable effect at 8 μmol/l and a 10-fold increase at 40 μmol/l. (diabetesjournals.org)
  • FGF-21 promotes insulin-independent glucose uptake and lipid accumulation in primary human adipocytes and in mouse 3T3-L1 cells. (stemcell.com)
  • Adipocyte differentiation, known as adipogenesis also, is an activity where fibroblast-like preadipocytes become adult adipocytes [6]. (euromedh2020.com)
  • Thus, in both differentiating and differentiated 3T3-L1 adipocytes, high concentrations of melatonin increased intracytoplasmic triglyceride accumulation, an indicator of adipogenic differentiation. (unican.es)
  • These findings suggest that, in human breast tumors, melatonin could stimulate the differentiation of fibroblasts and reduce the aromatase activity and expression in both fibroblasts and adipocytes, thereby reducing the number of estrogen-producing cells proximal to malignant cells. (unican.es)
  • Fibroblast-like STAT6 Purity & Documentation preadipocytes could differentiate to mature adipocytes accompanied with marked up-regulation with the differentiation markers and enhance of intercellular lipid accumulation (data not shown). (flapinhibitor.com)
  • Various cell types, including macrophages, fibroblasts, adipocytes, and vascular smooth muscle cells, were used to test the inhibitory effect of pioglitazone, a PPARγ agonist, on the expression of elastolytic enzymes. (ncku.edu.tw)
  • In vitro, pioglitazone downregulated Ctss and Mmp12 in macrophages, fibroblasts, and adipocytes-cell types residing within the adventitia and PVAT. (ncku.edu.tw)
  • Mammary epithelium differentiates in a stromal milieu of adipocytes and fibroblasts. (silverchair.com)
  • To investigate cell-cell interactions that may influence mammary epithelial cell differentiation, we developed a co-culture system of murine mammary epithelium and adipocytes and other fibroblasts. (silverchair.com)
  • Mammary epithelium from mid-pregnant mice was plated on confluent irradiated monolayers of 3T3-L1 cells, a subclone of the Swiss 3T3 cell line that differentiates into adipocytes in monolayer culture and other cell monolayers (3T3-C2 cells, Swiss 3T3 cells, and human foreskin fibroblasts). (silverchair.com)
  • Extract via Modulation of GLUT4 and PPAR-γ mRNA Expression in 3T3-L1 Adipocytes. (mercubuana-yogya.ac.id)
  • Our in vitro study detected a differentiation-dependent expression of FGF21 in 3T3-L1 adipocytes and human adipocytes. (edu.au)
  • The process of adipogenesis, and the start of differentiation can be induced in 3T3-L1 cells through the addition of compounds such as a synthetic glucocorticoid, a phosphodiesterase inhibitor, and insulin. (wikipedia.org)
  • Since 3T3-L1 cells are an immortalized cell line and require a hormonal differentiation, there has been much debate on their comparability to in vivo studies and primary cell lines. (wikipedia.org)
  • Aside from the synthetic differentiation itself, 3T3-L1 lineages can display low differentiation efficiency when utilizing common differentiation methods. (wikipedia.org)
  • 2014, Vol. 10 ECM expression in cultured adipocytesTo talk about the in vivo regional variations and alteration of ECM expression, in vitro gene expression in adipocyte differentiation was investigated applying 3T3-L1 cells (Fig. 6). (flapinhibitor.com)
  • Differentiation of 3T3-L1 murine fibroblasts into adipocyte-like cells was conducted in presence of IBMX, dexamethasone and insulin and demonstrated by Oil Red O staining of accumulated lipid droplets. (pensoft.net)
  • Swiss 3T3 cells, newborn foreskin fibroblasts, substrate-attached material ("extracellular matrix"), and tissue culture plastic did not support casein synthesis, whereas monolayers of 3T3-L1 and 3T3-C2 cells, a subclone of Swiss 3T3 cells that does not undergo adipocyte differentiation, did. (silverchair.com)
  • In addition to TSA, the HDAC inhibitors Scriptaid as well as the recently FDA-approved inhibitor SAHA robustly clogged adipocyte differentiation in 3T3-L1 cells (Figs. 1and supplemental Fig. S1and differentiation systems. (cancer-ecosystem.com)
  • Different HDAC inhibitors (TSA, Scriptaid, and SAHA) block adipocyte differentiation in either 3T3-L1 cells (and and and and and supplemental Fig. S1and and was improved upon TSA treatment (Fig. 3and and and in are representative of the duration of the TSA treatment. (cancer-ecosystem.com)
  • Treatment of 3T3-L1 cells induced to differentiate with 100 nm TSA causes the almost complete block of adipocyte differentiation. (cancer-ecosystem.com)
  • Moreover, proliferating cell nuclear antigen (PCNA) and CD31 showed co-localization with α-SMA, suggesting the differentiation of hBMSCs into epithelial cells and myofibroblasts/fibroblasts. (ijbs.com)
  • Peak Serum's US Origin FBS, (PS-FB1) worked better than Gibco on our 3T3-L1 cells / differentiation application. (peakfbs.com)
  • Methods 3T3‐L1 preadipocytes were differentiated by using a mixture of adipogenic inducers, including a suboptimal concentration of insulin. (researchgate.net)
  • jujubarepresses adipogenesis in 3T3-L1 preadipocytes [16]. (euromedh2020.com)
  • 3T3-L1 preadipocytes are useful model for physiological, pharmacological and cell signaling studies. (pensoft.net)
  • Several models exist to study adipogenesis in vitro , of which the cell line 3T3-L1 is the most well known, albeit not the most physiologically appropriate. (biomedcentral.com)
  • 3T3-L1 cells are utilized extensively like a cell tradition model to review the molecular control of adipogenesis [5]. (euromedh2020.com)
  • two main regulators of terminal adipogenesis, in 3T3-L1 cells. (unican.es)
  • Incubation of 3T3-L1 cells with TSA (100 nm) for the 1st 48 h of adipogenesis induction mediated most of the inhibitory effect. (cancer-ecosystem.com)
  • We confirmed our initial observation, namely that TSA blocks adipogenesis in the 3T3-L1 model, by using different HDAC inhibitors (TSA, SAHA, and Scriptaid) in two different models (3T3-L1 cells and mouse embryonic fibroblasts). (cancer-ecosystem.com)
  • Indeed, when 3T3-L1 cells were treated with valproic acid and butyrate we also observed improved adipogenesis. (cancer-ecosystem.com)
  • We show here that TGF-β stimulates murine 3T3-L1 fibroblasts to synthesize FBLN-5 transcript and protein through a Smad3-independent pathway. (elsevierpure.com)
  • The 3T3 original cell line was isolated from a mouse embryo and propagated for 3this specific line of 3T3 cells is used to study adipose tissuerelated diseases and dysfunctions. (wikipedia.org)
  • 3T3-L1 cells can be utilized to study a number of cellular and molecular mechanisms related to insulin-resistance, obesity, and diabetes in vitro. (wikipedia.org)
  • 3T3-L1 cells of the adipocyte morphology increase the synthesis and accumulation of triglycerides and acquire the signet ring appearance of adipose cells. (wikipedia.org)
  • The 3T3-L1 cell line is a sub clone that was initially developed from a mouse embryo, from a clonal expansion of Swiss 3T3 cells. (wikipedia.org)
  • The original cell line was developed through the 3T3 process, which is where the cells derive their naming nomenclature. (wikipedia.org)
  • The 3T3 process began with cultures having 3 days to propagate on a plate (the first "3"), and then a transfer (the "T") of 300,000 cells (second "3") to a new plate to restart the process. (wikipedia.org)
  • These cells will be identified as 3T3 only after they are taken through 20 to 30 passages and have established at a stable growth rate. (wikipedia.org)
  • The cells were cultured in a serum-containing medium and demonstrated a fibroblast-like morphology. (wikipedia.org)
  • 3T3-L1 cells, similar to their other 3T3 counterparts, are typically propagated as an adherent monolayer within a culture vessel. (wikipedia.org)
  • Basal cell culture medias for 3T3-L1 cells tends to contain a version of Dubelcco's Modified Eagle's Medium (DMEM), fetal bovine serum (FBS) , and an antibiotic at specific levels dependent upon protocol. (wikipedia.org)
  • Previous studies have found that DIM can improve type 2 diabetes by enhancing glucose uptake through the activation of insulin signaling in 3T3-L1 cells, and by lowering the plasma glucose levels in high-fat-diet-fed obese mice [13, 14]. (researchgate.net)
  • DIM, a major metabolite of indole-3-carbinol, which is naturally produced in broccoli and cabbage, enhances glucose uptake through the improvement of insulin sensitivity in 3T3-L1 cells [13] . (researchgate.net)
  • A) The biological activity of Mouse Recombinant FGF-21 was tested by its ability to promote the proliferation of NIH-3T3 cells in the presence of 1.25 μg/mL mouse Klotho and 10 μg/mL heparin. (stemcell.com)
  • Overexpression of FBLN-5 in 3T3-L1 cells increased DNA synthesis and enhanced basal and TGF-β-stimulated activation of ERK1/ERK2 and p38 mitogen-activated protein kinase (MAPK). (elsevierpure.com)
  • Unlike its proliferative response in fibroblasts, FBLN-5 overexpression in mink lung Mv1Lu epithelial cells resulted in an antiproliferative response, reducing their DNA synthesis and cyclin A expression. (elsevierpure.com)
  • Ultimately, several PPARγ binding sites were found in Ctss and Mmp12 in Raw 264.7 and 3T3-L1 cells, suggesting a direct regulatory mechanism by which PPARγ activation repressed the expression of Ctss and Mmp-12 in macrophages and fibroblasts. (ncku.edu.tw)
  • Ilieva B, Marinova E, Gagov H, Konakchieva R (2017) Basal and PGF 2α -stimulated secretion of pro-inflammatory cytokines from 3T3-L1 adipocyte-like cells. (pensoft.net)
  • It is concluded that pro-inflammatory phenotype of differentiated 3T3-L1 adipocyte-like cells, induced by PGF 2α is characterized by enhanced TNF α production which critically depends on the ability of CSE to produce H 2 S. (pensoft.net)
  • In a 24-h period, mammary epithelium co-cultured with 3T3-L1 cells secreted 12-20 micrograms beta-casein per culture dish. (silverchair.com)
  • Quantification of the extra fat accumulated in 3T3-L1 cells was performed by ORO staining and resolubilization of the dye bound to the lipid droplets within the cells with isopropanol. (cancer-ecosystem.com)
  • Here, Park and Jeong took advantage of previously identified stem cell transcription factors associated with potency to differentiate umbilical cord mesenchymal stem cells (US-MSCs) from morphologically similar fibroblasts. (emerginginvestigators.org)
  • We studied whether cellular senescence induced by HDAC inhibitor sodium butyrate in E1a+cHa-Ras -transformed rat embryo fibroblasts (ERas) and A549 human Ki-Ras mutated lung adenocarcinoma cells would enhance the tumor suppressor effect of MEK/ERK inhibition. (aging-us.com)
  • The 3T3-L1 Swiss sub clone line has been widely utilized, since its development, due to its affinity for lipid droplet deposition in vitro. (wikipedia.org)
  • OBJECTIVE-Fibroblast growth factor 21 (FGF21) is a metabolic regulator with multiple beneficial effects on glucose homeostasis and insulin sensitivity in animal models. (edu.au)
  • Fibroblast growth factor 21 (FGF-21) is a member of the FGF family. (stemcell.com)
  • Fibroblast growth factor 11 (FGF11) is a member of the intracellular fibroblast growth factor family. (biomedcentral.com)
  • We compared the growth of three different primary fibroblast lines in media supplemented with Peak Serum's FBS to their growth in media supplemented with four other commercially-available FBS samples and noticed a big difference in growth or cell morphology by phase microscopy when using Peak Serum's FBS. (peakfbs.com)
  • In 1962, the original 3T3 cell line that it was established by George Todaro and Howard Green of the New York University School of Medicine. (wikipedia.org)
  • Mouse embryonic fibroblasts nourish embryonic stem cells by secreting growth factors in addition to providing a cellular matrix to promote cell growth. (altogen.com)
  • Embryonic stem cells are a widely-used means for cell and molecular biology research, and mouse fibroblast cell lines are imperative for the proper growth of stem cells. (altogen.com)
  • The researchers confirmed these findings in cultured 3T3-L1 cells, an MEF adipose like cell line that can be triggered to differentiate into adipocyte-like cells. (genengnews.com)
  • The BALB cell line (also known as 3T3 clone A31) was established in 1968 from fibroblast tissues found in BALB/c mouse (Mus musculus) embryos. (altogen.com)
  • We compared the growth of three different primary fibroblast lines in media supplemented with Peak Serum's FBS to their growth in media supplemented with four other commercially-available FBS samples and noticed a big difference in growth or cell morphology by phase microscopy when using Peak Serum's FBS. (peakfbs.com)
  • In 1962, the original 3T3 cell line that it was established by George Todaro and Howard Green of the New York University School of Medicine. (wikipedia.org)
  • Línea celular establecida en 1962 a partir de embriones desagregados de ratón albino suizo. (bvsalud.org)
  • Exposure of quiescent cultures of human gingival fibroblasts (HuGi) and porcine synoviocytes (PSF) to human recombinant interleukin 1.alpha. (eurekamag.com)
  • Conclusion: The present study demonstrated that the presence of a high number of HSP47-positive fibroblasts in the cancer stroma was a risk factor for recurrence of lung cancer after surgery. (researchsquare.com)