• In addition, OBEX induced a clear reduction of the lipid load in mature adipocytes obtained from 3T3-F442A cells. (nature.com)
  • In addition, its action on the 3T3-F442A cell line was tested in terms of regulation of proliferation capacity and differentiation into mature adipocytes as well as its effects on mature adipocytes. (nature.com)
  • In addition, on the 3T3-F442A cell line, OBEX inhibited proliferation of pre-adipocytes, blocked the differentiation of pre-adipocytes into new adipocytes, and decreased the lipid load in mature adipocytes. (nature.com)
  • PBM-treated hypertrophic cells (1064 nm and 17.6 J/cm2 every day for 7 days following addition of PA) decreased the lipid levels in hypertrophic adipocytes, restored the GLUT4 protein expression and enhanced glucose transport. (google.com)
  • Adipocytes were recently identified as an important source of endocrine and paracrine mediators, regulating the metabolism and activity of various cell types and body functions. (pensoft.net)
  • KSS inhibited the accumulation of triglycerides in a dose-dependent manner in 3T3-L1 cells that were induced to differentiate into adipocytes. (elsevierpure.com)
  • This study aimed to determine whether I3C or DIM could increase glucose uptake via enhanced insulin sensitivity in 3T3‐L1 adipocytes, as well as the mechanism involved. (researchgate.net)
  • 3,3′-diindolylmethane (DIM)-a natural compound produced from indole-3-carbinol, found in cruciferous vegetables-enhances glucose uptake by increasing the activation of the insulin signaling pathway in 3T3-L1 adipocytes. (researchgate.net)
  • In IR adipocytes, uptake of D3 and 25-hydroxyvitamin-D3 was higher, but, after adrenaline stimulation, the decrement in D3 and 25-hydroxyvitamin-D3 was stronger in control cells, which also showed increased expression of Cyp27a1 and Cyp27b1 and higher levels of 25-hydroxyvitamin-D3. (medscape.com)
  • 3T3 cells are several cell lines of mouse embryonic fibroblasts. (wikipedia.org)
  • Second messengers regulate endosomal acidification in Swiss 3T3 fibroblasts. (rupress.org)
  • Fluorescent probes and imaging methods were developed to measure pH to better than 0.2 U accuracy in individual endocytic vesicles in Swiss 3T3 fibroblasts. (rupress.org)
  • Differentiation of 3T3-L1 murine fibroblasts into adipocyte-like cells was conducted in presence of IBMX, dexamethasone and insulin and demonstrated by Oil Red O staining of accumulated lipid droplets. (pensoft.net)
  • HFKs were co-cultured with irradiated (3,000 Rad) J2 cells (kindly provided by Dr. Richard Schlegel, Georgetown University or college) derived from mouse NIH 3T3 fibroblasts at a 1:4 HFK:J2 cell percentage, and sub-cultured when they reached 85% confluence. (cell-metabolism.com)
  • Plates containing confluent 3T3 fibroblasts were exposed to the various media for 24h, at 37°C with 5% CO2, and cell viability was evaluated by a multiparametric assay assessing sequentially, on the same cells, mitochondrial activity (XTT), membrane integrity (neutral red test), and total cell density (crystal violet dye exclusion test). (bvsalud.org)
  • This study shows that whole milk and HBSS are more efficient in maintaining the viability of 3T3 fibroblasts as demonstrated by three different cell viability tests. (bvsalud.org)
  • tooth avulsion, fibroblasts, cell viability. (bvsalud.org)
  • Cytocompatibility was assessed by a multiparametric assay employing 3T3 fibroblasts. (bvsalud.org)
  • Changes in the expression of proteins related to adipocyte differentiation in 3T3-L1 cells were determined by SDS-PAGE and western blotting. (foodandnutritionresearch.net)
  • Discussion : Taken together, the results of this study demonstrate that TME inhibits lipid accumulation and reduces the expression PPARG, CEBPA, and SREBF1, which regulate adipocyte differentiation in 3T3-L1 cells. (foodandnutritionresearch.net)
  • In vitro analysis with 3T3-F442A cells revealed anti-proliferative and anti-differentiation effects of OBEX. (nature.com)
  • The morphological differentiation of 3T3-L1 preadipocytes includes the loss of "stress fibers" and the appearance of microfilament like structures that encase, in a complex manner, the cytosolic lipid spheres that appear during differentiation. (silverchair.com)
  • Here, we demonstrate the molecular mechanism underlying the effect of KSS on the differentiation of mouse preadipocytes (3T3-L1 cells). (elsevierpure.com)
  • Pimozide, a novel fatty acid binding protein 4 inhibitor, promotes adipogenesis of 3T3-L1 cells by activating PPARγ. (ox.ac.uk)
  • [ 16 ] Potential mechanisms through which TCF7L2 variants influence T2DM include its role in adipogenesis, myogenesis, and pancreatic islet development, as well as in beta-cell survival and insulin secretory granule function. (medscape.com)
  • [ 17 , 18 ] TCF7L2 protein is increased during adipogenesis in 3T3-L1 cells and in primary adipocyte stem cells. (medscape.com)
  • Organelle relationships in cultured 3T3-L1 preadipocytes. (silverchair.com)
  • 3T3-L1 preadipocytes are useful model for physiological, pharmacological and cell signaling studies. (pensoft.net)
  • Methods 3T3‐L1 preadipocytes were differentiated by using a mixture of adipogenic inducers, including a suboptimal concentration of insulin. (researchgate.net)
  • Epithelial features of 'nonproducer' balb/3t3 cells transformed by mur" by Y Ikawa, A F. Gazdar et al. (jax.org)
  • Epithelial features of 'nonproducer' balb/3t3 cells transformed by murine sarcoma virus. (jax.org)
  • Using purified terminal complement components we have documented a mitogenic effect of the MAC for quiescent murine 3T3 cells. (jci.org)
  • At the time of their establishment, 3T3 cells were different than most other cell lines in regard to the fact they did not induce tumors to develop when injected into murine species. (fsu.edu)
  • Analysis of purified magnetically labeled TNFR1 complexes from murine and human cells stably transduced with 14.7K revealed that prevention of TNFR1 internalization resulted in inhibition of DISC formation. (jci.org)
  • The 3T3 lines are valuable in vitro host systems for oncogenic virus transformation studies, since 3T3 cells possess a high sensitivity to CONTACT INHIBITION. (jefferson.edu)
  • NiL showed better cytotoxicity on both cancerous cells relative to normal cells in vitro with the highest selective index of 2.22 in MCF-7 cells. (ac.ir)
  • In addition, NK cells stimulated with lunasin plus cytokines displayed higher tumoricidal activity than those stimulated with cytokines alone using in vitro and in vivo tumor models. (weeksmd.com)
  • Ésta línea es un sistema hospedador in vitro de gran valor para los estudios de transformación de virus oncogénicos, ya que las células 3T3 poseen una elevada sensibilidad a la inhibición por contacto. (bvsalud.org)
  • The international EU/COLIPA in vitro phototoxicity validation study - results of phase II (blind trial) - Part 1 - The 3T3 NRU phototoxicity test. (medscape.com)
  • In differentiating 3T3-L1 cells, lipid spheres, the endoplasmic reticulum (ER), microperoxisomes, and mitochondria form "constellations" that may reflect the interplay of lipid metabolizing enzymes in these organelles. (silverchair.com)
  • As in hepatocytes and absorptive cells of the intestine, this spatial relationship of ER and plasmalemma suggests a role for rosettes in the uptake of exogenous lipid precursors. (silverchair.com)
  • Established by George Todaro and Howard Green in 1962 from disaggregated Swiss mouse ( Mus musculus ) embryo tissue, the 3T3 cell line is a standard fibroblast cell line used in a wide spectrum of research and industrial biomedical applications. (fsu.edu)
  • Mouse Embryo Fibroblast Cells with Texas Red-X, Alexa Fluor 488, and DAPI - In this section, a Swiss mouse embryo fibroblast cell is presented that was resident in a culture labeled with the fluorophore Texas Red-X conjugated to wheat germ agglutinin, a fluorescent lectin that selectively binds to sialic acid residues. (fsu.edu)
  • The prepared chitin/gelatin membranes with or without GlcNAc were characterized by mechanical, swelling, enzymatic degradation, thermal and growth of NIH/3T3 fibroblast cell studies. (amrita.edu)
  • Previous studies have found that DIM can improve type 2 diabetes by enhancing glucose uptake through the activation of insulin signaling in 3T3-L1 cells, and by lowering the plasma glucose levels in high-fat-diet-fed obese mice [13, 14]. (researchgate.net)
  • DIM, a major metabolite of indole-3-carbinol, which is naturally produced in broccoli and cabbage, enhances glucose uptake through the improvement of insulin sensitivity in 3T3-L1 cells [13] . (researchgate.net)
  • Finally, TCF7L2 polymorphisms have been associated with impaired insulin secretion, glucose production, and glucose tolerance via direct effects on pancreatic islet beta cells. (medscape.com)
  • A confluent monolayer yields 40000 cells/cm2. (wikipedia.org)
  • Contact inhibited, a confluent monolayer of 3T3 cells yields approximately 40,000 cells per square centimeter. (fsu.edu)
  • However, due to examination of 3T3 cells and subsequent research it has become widely accepted that for immortalization of cells to take place, telomere shortening, which can instigate chromosomal rearrangements, must be overcome, a process that is not necessarily related to a cell s ability to undergo oncogenic transformation. (fsu.edu)
  • Studies have been performed to determine if cadmium chloride (CdCl(2)) can induce morphological cell transformation, DNA from CdCl(2)-induced transformed cells can transform other mammalian cells, and the transformed cells induced by CdCl(2) can form tumors in nude mice. (cdc.gov)
  • DNA from all five CdCl(2)-induced transformed cell lines tested was found to induce varying degrees of transfection-mediated transformation in NIH-3T3 cells. (cdc.gov)
  • These results indicate that CdCl(2) is capable of inducing morphological cell transformation and that the transformed cells induced by CdCl(2) are potentially tumorigenic. (cdc.gov)
  • Todaro and Green originally obtained their 3T3 cells from Swiss albino mouse embryo tissue. (wikipedia.org)
  • Activation of cdc42 in Swiss 3T3 cells leads to the sequential activation of rac and then rho, suggesting a molecular model for the coordinated control of cell motility by members of the rho family of GTPases. (nih.gov)
  • Using gene manifestation profiling, practical siRNA library testing and reverse-phase protein arrays (RPPA), we buy Dihydrotanshinone I found that J2 cells and Y produced a cooperative effect on human being foreskin keratinocytes (HFKs), which resulted in suppression of TGF- signaling juxtaposed with up-regulation of changes in cell cycle progression, cell adhesion and motility. (cell-metabolism.com)
  • Using flow cytofluorimetry sorting of populations of exponentially growing cells, coupled to a radioimmune assay, we have investigated the accumulation of p53 along the cell cycle in normal FR 3T3 rat cells as well as in two types of SV40-transformed derivatives, one of which only expresses the large-T protein during the G2 phase of the cell cycle. (crcm-marseille.fr)
  • DNAs from five CdCl(2)-induced transformed cell lines were isolated and gene transfection assay was performed using NIH-3T3 cells. (cdc.gov)
  • The simultaneous evaluation of different cell viability parameters, in a multiparametric assay, may identify more accurately any possible cytotoxic effects of storage media with immortalized cells intimately related to the in-vivo tissue. (bvsalud.org)
  • The original 3T3 cell line (3T3-Swiss albino) was established in 1962 by two scientists then at the Department of Pathology in the New York University School of Medicine, George Todaro and Howard Green. (wikipedia.org)
  • Later, as a principal investigator position at the National Cancer Institute in Bethesda, Maryland, Todaro repeated the isolation procedure from the NIH Swiss mouse embryo with his students and established NIH-3T3 cell line. (wikipedia.org)
  • Swiss Mouse Embryo Fibroblast Cells with MitoTracker Red CMXRos, Alexa Fluor 488, and DAPI - A log phase culture of embryonic Swiss mouse fibroblast cells was stained with MitoTracker Red CMXRos, Alexa Fluor 488 conjugated to phalloidin, and DAPI, which target the intracellular mitochondrial network, cytoskeletal actin filaments, and nuclei, respectively. (fsu.edu)
  • Visualizing Structural Features of the Golgi Complex and Nucleus in Swiss Mouse Embryo Cells - In order to examine structural features of the Golgi complex and nucleus at relatively high magnification, a log-phase culture of 3T3 cells was fixed, permeabilized, blocked with normal goat serum, and then treated with rabbit anti-giantin (Golgi protein) primary antibodies followed by goat anti-rabbit secondary antibodies (IgG) conjugated to Alexa Fluor 568. (fsu.edu)
  • Adherent cells grow as a monolayer. (wikipedia.org)
  • RESEARCH particular, timing of return to roost, leaving for feeding, monolayer of Vero cells. (cdc.gov)
  • 3T3 Cells" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (jefferson.edu)
  • Adding lunasin to IL-12- or IL-2-stimulated NK cells demonstrated synergistic effects in the induction of IFNG and GZMB involved in cytotoxicity. (weeksmd.com)
  • To evaluate the cytotoxicity of several storage media (coconut water, whole milk, isotonic solution, Hank´s Balanced Salt Solution and tap water) using a multiparametric cytotoxicity analysis employing 3T3 cells. (bvsalud.org)
  • Variants of the initial cell line have been tested and found negative for ectromelia virus (mousepox), but most are susceptible to polyoma and simian virus 40 ( SV40 ). (fsu.edu)
  • previous to studies of the 3T3 line, it was believed that these characteristics were necessarily synonymous with one another. (fsu.edu)
  • Colocalisation studies in cells were performed by double staining with antibodies against human cystatin C and marker proteins for lysosomes, the Golgi apparatus, or the endoplasmic reticulum, and evaluated by confocal microscopy. (lu.se)
  • Eukaryotic expression vectors containing genes encoding plant proteins for killing of cancer cells. (weeksmd.com)
  • consequently, genes encoding some of these proteins are being used to design constructs for the inhibition of multiplying cancer cells. (weeksmd.com)
  • Because ion fluxes and cytosolic ionic changes are integral steps in the signaling cascade initiated when growth factors bind to their receptors, we hypothesized that the MAC-induced reversible changes in membrane permeability could stimulate cell proliferation. (jci.org)
  • The MAC enhances the mitogenic effects of serum and PDGF, and also stimulates cell proliferation in the absence of other exogenous growth factors. (jci.org)
  • MAC-induced mitogenesis represents a novel effect of the terminal complement complex that could contribute to focal tissue repair or pathological cell proliferation locally at sites of complement activation. (jci.org)
  • Low levels of ROS can promote cell proliferation but high levels induce cell death [ 13 ]. (biomedcentral.com)
  • The p53 oncogene is thought to play a role in the proliferation of normal and transformed cells and its expression was postulated to be cell cycle dependent. (crcm-marseille.fr)
  • 3T3 cells can be transformed with SV40 and some other polyomaviruses. (wikipedia.org)
  • With this goal in mind, we wanted to determine the signaling pathways associated with J2 cells and Y that are paramount to the immortalization process. (cell-metabolism.com)
  • Co-culture of human being main epithelial cells with irradiated 3T3 fibroblast feeder cells (J2 cells) and the Rho kinase inhibitor Y-27632 (Y) allows for the unrestricted growth of cells of epithelial source by the process termed conditional reprogramming. (cell-metabolism.com)
  • Recently, this co-culture technique was altered to include epithelial cells from prostate, buy Dihydrotanshinone I breast, trachea, liver and lung, wherein the use of J2 cells and the Rho kinase inhibitor Y-27632 (Y) resulted in the quick reprogramming of cells into immortalized karyotype-stable ethnicities [6, 7]. (cell-metabolism.com)
  • These conditionally reprogrammed epithelial cells indicated markers of adult stem cells, but not of embryonic and induced pluripotent stem cells, and appeared to consist of cell populations resembling their main tissue of source, making them ideal to study tissue regeneration, as well as normal physiologic and pathologic processes [8]. (cell-metabolism.com)
  • The primary mouse embryonic fibroblast cells were transferred (the "T") every 3 days (the first "3"), and inoculated at the rigid density of 3×105 cells per 20 cm2 dish (the second "3") continuously. (wikipedia.org)
  • Evaluated by Western Blot in NIH/3T3 cell lysate. (sigmaaldrich.com)
  • Western Blot Analysis: A 1:500 dilution of this antibody detected VEGF on 10 µg of NIH/3T3 cell lysate. (sigmaaldrich.com)
  • Flow Cyt: Wild-type HAP1, HeLa and NIH/3T3 cells. (abcam.com)
  • IP: HeLa and NIH/3T3 cells. (abcam.com)
  • In addition to inducing necrosis and apoptosis, ROS induces autophagic cell death. (biomedcentral.com)
  • 14.7K protects NIH 3T3 cells from TNF-induced apoptosis. (jci.org)
  • Ilieva B, Marinova E, Gagov H, Konakchieva R (2017) Basal and PGF 2α -stimulated secretion of pro-inflammatory cytokines from 3T3-L1 adipocyte-like cells. (pensoft.net)
  • The expression of VEGF is potentiated and is secreted by tumor cells in response to hypoxia, by activated oncogenes, and by a variety of cytokines. (sigmaaldrich.com)
  • With the goal of further improving cytokine-based immunotherapy, we examined the effects of a soybean peptide called lunasin that synergistically works with cytokines on natural killer (NK) cells. (weeksmd.com)
  • The combination of lunasin and cytokines (IL-12 plus IL-2) was capable of restoring IFNγ production by NK cells from post-transplant lymphoma patients. (weeksmd.com)
  • J. Cell Biol. (wikipedia.org)
  • J Cell Biol (1992) 119 (1): 99-110. (rupress.org)
  • Cell lines whose original growing procedure consisted being transferred (T) every 3 days and plated at 300,000 cells per plate (J Cell Biol 17:299-313, 1963). (jefferson.edu)
  • Int J Biochem Cell Biol. (jefferson.edu)
  • J Cell Biol (1980) 87 (1): 180-196. (silverchair.com)
  • METHODS: Expression vectors for wild-type and L68Q cystatin C were constructed and used to transfect mouse NIH/3T3 cells. (lu.se)
  • Permanent expression of p53 in FR 3T3 rat cells but cell cycle-dependent association with large-T antigen in simian virus 40 transformants. (crcm-marseille.fr)
  • in both IR cells and obese SAT, protein expression of β 2 -adrenergic receptor was reduced. (medscape.com)
  • Design : 3T3-L1 cells were differentiated in the presence of different concentrations of TME. (foodandnutritionresearch.net)
  • BALB/c-3T3 cells were treated with different concentrations of CdCl(2) for 72 h. (cdc.gov)
  • The most commonly studied isoforms are VEGF121, VEGF165, and VEGF189 (f, d and b, respectively) All seven isoforms differ in their molecular mass and in biological properties such as their ability to bind to cell-surface heparan-sulfate proteoglycans. (sigmaaldrich.com)
  • Furthermore, we found that the same TNF defense mechanisms were instrumental in protecting wild-type adenovirus-infected human cells expressing 14.7K. This study describes a new molecular mechanism implemented by a virus to escape immunosurveillance by selectively targeting TNFR1 endocytosis to prevent TNF-induced DISC formation. (jci.org)
  • Non-transformed BALB/c-3T3 cells and cells from 10 transformed cell lines induced by CdCl(2) were injected into both axillary regions of nude mice. (cdc.gov)
  • All 10 CdCl(2)-induced transformed cell lines formed fibrosarcomas in nude mice within 39 days of inoculation. (cdc.gov)
  • Within this time period, no tumors were found in nude mice injected with non-transformed BALB/c-3T3 cells. (cdc.gov)
  • The membrane attack complex of complement (MAC) can induce reversible changes in cell membrane permeability resulting in significant but transient intracellular ionic changes in the absence of cell lysis. (jci.org)
  • Regarding the phenomenon of cellular guidance itself, 3T3 cells faced with a choice between guiding lines toward different directions seem to explore various options before following one. (northwestern.edu)
  • One such peptide, Lunasin , has been shown to selectively act on newly transformed cells while having no cytotoxic effect on non-tumorigenic or established cancer cell lines. (weeksmd.com)
  • NIH 3T3 and C127 cells expressing either PM-14.7K ( A and C ) or 14.7K ( B and D ) were treated with 100 ng/ml TNF for 18 hours in the absence (bold lines) or presence of 12.5 μg/ml CHX (dotted lines). (jci.org)
  • Taken together, PBM is shown capable of restoring the cellular morphology and function of hypertrophic cells. (google.com)
  • Metformin (MTF) has been reported to target NLK (Nemo-like kinase) to inhibit non-small lung cancer cells. (cancerindex.org)
  • RESULTS: Concentrations of human cystatin C secreted from transfected NIH/3T3 cells were similar to those secreted from human cells in culture. (lu.se)
  • Evidence for GO induced autophagy includes increased levels of isoform B-II microtubule-associated protein 1 light chain 3 (LC3), aggregation of green fluorescence protein-tagged LC3, and increased numbers of autophagic vacuoles in cells. (biomedcentral.com)
  • In general, clones expressing the gene encoding L68Q cystatin C secreted slightly lower amounts of the protein than clones expressing wild-type human cystatin C. Both immunofluorescence cytochemistry and western blotting experiments showed an increased accumulation of cystatin C in cells expressing the gene encoding L68Q cystatin C compared with cells expressing the gene for the wild-type protein. (lu.se)
  • Overexpression of IRS-1 resulted in inhibition of basal autophagy, and reduced oxidative stress-induced autophagy and cell death. (biomedcentral.com)
  • Knockdown of autophagy-related gene 5 inhibited basal autophagy and diminished oxidative stress-induced autophagy and cell death. (biomedcentral.com)
  • Our results suggest that overexpression of IRS-1 promotes cells growth, inhibits basal autophagy, reduces oxidative stress-induced autophagy, and diminishes oxidative stress-mediated autophagy-dependent cell death. (biomedcentral.com)
  • Journal of Cell Biology , 80 (1), 53-60. (northwestern.edu)
  • Albrecht-Buehler, G 1979, ' The angular distribution of directional changes of guided 3T3 cells ', Journal of Cell Biology , vol. 80, no. 1, pp. 53-60. (northwestern.edu)
  • When dental avulsion occurs, immediate replantation at the trauma site is the ideal procedure for maintaining the viability of periodontal ligament (PDL) cells. (bvsalud.org)
  • The viability of PDL cells relies on three critical factors: the duration of extra-alveolar time, preservation of the root and storage media of the tooth, all of which determine the prognosis for dental replantation 2-5 . (bvsalud.org)
  • Thus, to avoid further damage do the PDL cells, it is important to select an appropriate storage medium to keep their viability. (bvsalud.org)
  • Autophagy usually serves as a survival mechanism in response to stress conditions, but excessive induction of autophagy results in cell death. (biomedcentral.com)
  • The underlying mechanism responsible for the effects of lunasin on NK cells is likely due to epigenetic modulation on target gene loci. (weeksmd.com)
  • siRNA screening The siRNA library to assess factors secreted by J2 cells was comprised of 332 fully annotated genes based on the 3T3 cell transcriptome in GEO database "type":"entrez-geo","attrs":"text":"GSM1348503″,"term_id":"1348503″GSM1348503. (cell-metabolism.com)
  • Genome_build: Concatenated UCSC hg38 and mm10 for U87-3T3 cell mixture scRNA-Seq profiles. (nih.gov)
  • Survey experience is sufficient to conclude that laboratory aerosol infection does not occur in the course of routine work with cell cultures and animals not subject to chronic infection. (cdc.gov)
  • The spontaneously immortalized cells with stable growth rate were established after 20 to 30 generations in culture, and then named '3T3' cells. (wikipedia.org)
  • Stable cell clones were isolated after cotransfection with pSV2neo. (lu.se)
  • Materials and Methods Cells Human being keratinocytes were cultured from discarded and de-identified neonatal foreskin cells. (cell-metabolism.com)
  • However, the formation of complexes between p53 and large-T was modulated by the G2-restricted accumulation of large-T, thus leading to a differential increase in the levels of p53 both in exponentially growing cells and along the cell cycle. (crcm-marseille.fr)
  • Tubulin, Actin, and DNA Distribution in 3T3 Cells - A culture of 3T3 cells was immunofluorescently labeled with primary anti-tubulin mouse monoclonal antibodies followed by goat anti-mouse Fab fragments conjugated to the cyanine dye, Cy3. (fsu.edu)
  • Cells generate partially reduced forms of oxygen, collectively referred to as "reactive oxygen species" (ROS), during respiration and enzymatic processes. (biomedcentral.com)