GenesEnzymeGene2021CharacterizationCleavagePseudomonasCatabolicBacteriaPurificationSubstratesPathwayMolecularIsolateAcid2019ReactionEnzymesBioremediationProteinMechanismSynthetaseWastewaterHydrocarbonsDegradationProteinsAcetylOxygenCompoundsBiologyPyrene2022SynthaseCDNAAldehydeReductionSpecificityContaminantsMethyltransferaseTemperatureMicrobiologyProducts
Genes9
- nidA and nidB genes are encoding genes for large and small subunits of napthalene-inducible dioxygenase [ 7 , 8 ]. (omicsonline.org)
- The second ben/cat operon was located in a 92-kb scaffold along with (i) an operon ( opp ) comprising genes for the transformation of OPP to BA and 2-hydroxypenta-2,4-dienoate (and genes for its transformation) and (ii) an incomplete biphenyl catabolic operon ( bph ). (nature.com)
- Genes up-regulated in comparison of dendritic cells (DC) stimulated with Pam3Csk4 (TLR1/2 agonist) at 8 h versus DC cells stimulated with CpG DNA (TLR9 agonist) at 8 h. (gsea-msigdb.org)
- The existence of catabolic and surfactant genes namely catechol dioxygenase ( C23O ), rhamnolipid enzyme ( rhlB ) and surfactin /lichenysin enzyme ( SrfA3 /LicA3 ) genes were detected in only four (4) out of the nine (9) marine aromatic degrading bacteria with 881 base pairs sizes. (journalarrb.com)
- Here, we focus on the recently obtained knowledge considering structural genes as well as transcriptional and post-translational regulators involved in the metabolic pathways underlying both appearance and internal quality such as those involved in chlorophyll, carotenoid, cell wall, central and secondary metabolism (Table 1 , Figure 1 ). (biomedcentral.com)
- The gene encoding the Ov-phy-1 open reading frame contained 11 introns, similar in structure to the gene encoding human prolyl 4-hydroxylase isoform I. Genomic Southern blot, EST and genomic PCR studies demonstrated that the O. volvulus genome contained between three and eight genes closely related to Ov-phy-1. (embl.de)
- An A. salmonicida genomic DNA library was constructed by using lambda GEM-11 and recombinant phage carrying both genes ompAI and ompAII) selected by immunoscreening. (shengsci.com)
- Products of TET methylcytosine dioxygenase genes (and (and participate in DNA demethylation through DNA hydroxymethylation, deamination, foundation excision restoration (BER), and additional mechanisms [4, 13C19]. (edrc2013.org)
- Genes & Development 21 (3): 316-331. (mdc-berlin.de)
Enzyme9
- the 1,2-CTD enzyme produced by Pseudomonas arvilla is the exception to this rule, containing two highly homologous subunits that can form either a homo- or hetero- dimer. (wikipedia.org)
- Specific activities of Catechol-2,3-dioxygenase and effects of temperature and pH and their stabilities on the enzyme relative activities were observed. (openmicrobiologyjournal.com)
- PAHs could be degraded by bacteria under aerobic conditions through the initial oxidation of the aromatic ring, which is catalyzed by the dioxygenase enzyme. (omicsonline.org)
- The enzyme contained 2 g-atoms of iron per mol of protein. (shengsci.com)
- An enzyme of the oxidoreductase class that catalyzes the reaction between catechol and oxygen to yield benzoquinone and water. (lookformedical.com)
- this file supplies the natural data of preliminary experiments performed using 2 enzyme dilutions in order to discover out the dilution that greatest fitted with requirements 1 and 2 described in the outcomes portion of the manuscript (automated sugars releasing assay: substrate focus). (tam-receptor.com)
- Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human AE Binding Protein 1 (AEBP1) in tissue homogenates, cell lysates and other biological fluids. (kits-elisa.com)
- Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human AE Binding Protein 1 (AEBP1) in samples from tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species. (kits-elisa.com)
- Cell culture and in vitro studies show that fenretinide binds and inhibits the activity of ß-carotene oxygenase 1 (BCO1), the enzyme responsible for endogenous vitamin A formation. (bvsalud.org)
Gene6
- Moreover, a dioxygenase gene, nidA3B3, was detected in Mycobacterium vanbaalenii PYR-1 as an alternate degradation pathway which could catalyze both the initial dihydroxylation of pyrene [ 11 ] to be pyrene cis-4,5-dihydrodiol, and an alternate detoxification pyrene pathway to be pyrene cis-1,2-dihydrodiol [ 12 ]. (omicsonline.org)
- Catechol 2,3-dioxygenase (C23O) gene was found andamplified with the designed primers from the total DNA of C-14-1. (researchgate.net)
- The result of Southern blot indicated that there isonly one C23O gene in the genome of C-14-1. (researchgate.net)
- 6 identified a gene cluster, hbpCAD , encoding the upper metabolic pathway of OPP which involves the transformation of OPP to 2-hydroxypenta-2,4-dienoateand benzoic acid (BA). (nature.com)
- Complementation of the E. coli phr-1 mutation was observed, strongly suggesting that the yeast PHR1 gene encodes a. (shengsci.com)
- Finally, we examine the conservation of 135 USA300 TFs amongst 11 other S. aureus strains, identifying a key group of regulators that display a high degree of conservation, including many that have previously been demonstrated to play a role in virulence gene regulation. (biomedcentral.com)
20212
- 2021. Separate upper pathway ring cleavage dioxygenases are required for growth of Sphingomonas wittichii strain RW1 on dibenzofuran and dibenzo-p-dioxin. (nih.gov)
- 20(1): 126, 2021 Oct 03. (bvsalud.org)
Characterization2
- Substrate specificity of catechol oxidase from Lycopus europaeus and characterization of the bioproducts of enzymic caffeic acid oxidation. (lookformedical.com)
- 1. Fischer, D., Ebenau-Jehle, C. and Grisebach, H. Phytoalexin synthesis in soybean: purification and characterization of NADPH:2'-hydroxydaidzein oxidoreductase from elicitor-challenged soybean cell cultures. (qmul.ac.uk)
Cleavage2
- In the convergent mode, structurally diverse aromatic compounds are converted to one of a few aromatic ring cleavage substrates such as catechol, gent sate, protocatechuate and their derivatives [ 5 ]. (biomedcentral.com)
- In this divergent mode, a metal-dependent dioxygenase channels these dihydroxylated intermediates into one of the two possible pathways: the meta -cleavage pathway or the ortho -cleavage pathway [ 7 - 9 ] (Fig. 1 ). (biomedcentral.com)
Pseudomonas3
- Thus far, 1,2-CTD has been observed to exist in the following species of soil bacteria and fungi: Pseudomonas sp. (wikipedia.org)
- They isolated a Pseudomonas azelaica strain HPB1 which was able to degrade OPP through the production of 2,3-dihydroxybiphenyl. (nature.com)
- Metabolism of 2,4,6-trinitrotoluene by Pseudomonas sp. (paperbase.net)
Catabolic1
- Proteomics identified 13 up-regulated catabolic proteins when S. haloaromaticamans was growing on OPP and/or BA. (nature.com)
Bacteria2
- These bacteria subsequently employ 1,2-CTD in the last step of the degradation of aromatic compounds to aliphatic products. (wikipedia.org)
- A number of bacteria that can degrade a variety of aromatic compounds have been identified and the pathways involved in the degradation have been extensively characterized [ 3 , 4 ]. (biomedcentral.com)
Purification2
- Catechol 1,2-dioxygenase from Acinetobacter calcoaceticus: purification and properties. (shengsci.com)
- Procedures for the purification of catechol 1,2-dioxygenase from extracts of Acinetobacter calcoaceticus strain ADP-96 are described. (shengsci.com)
Substrates2
- Oxygenases belong to the oxidoreductive group of enzymes (E.C. Class 1), which oxidize the substrates by transferring oxygen from molecular oxygen (O 2 ) and utilize FAD/NADH/NADPH as the co-substrate. (biomedcentral.com)
- Substrates containing a -COOH group are inhibitors for catechol oxidase. (lookformedical.com)
Pathway1
- The pcaH was significant in the β-ketoadipate pathway [9] where the pathway as it conserves biochemically, and is a major class of non-heme-iron containing dioxygenase [ 10 ]. (omicsonline.org)
Molecular1
- low-molecular-weight (LMW) compounds consist of two or three rings, and high-molecular-weight (HMW) compounds, more than three rings [ 3 ]. (omicsonline.org)
Isolate2
- In the pyrene and fluoranthene degradation experiment using a minimum medium, B. fungorum isolate no. 1 and M. gilvum isolate no. 13 were able to degrade 98.6% + 1.9 of pyrene after 20 days, and 99.9% ± 0.1 after a 16-day incubation period, respectively. (omicsonline.org)
- Moreover, fluoranthene could be consumed by B. fungorum isolate no. 1 and M. gilvum isolate no. 13 at the level of 99.6% ± 0.7 after 24 days, and 100% after a 28-day incubation period, respectively. (omicsonline.org)
Acid5
- 1,2-CTD uses Fe3+ as a cofactor to cleave the carbon-carbon bond between the phenolic hydroxyl groups of catechol, thus yielding muconic acid as its product. (wikipedia.org)
- However, further conversion of these intermediates into tricarboxylic acid (TCA) cycle intermediates was found to be highly diverged (divergent mode) (Fig. 1 ). (biomedcentral.com)
- The derived amino acid sequence of Ov-phy-1 encoded a peptide that was most similar to the two Caenorhabditis elegans prolyl 4-hydroxylase homologues and to the isoform II enzymes of vertebrates. (embl.de)
- Two compounds, Compound 1 (1,4-dihydrophenanthrolin-4-one-3-carboxylic acid) and Compound 5 [8-(N-butyl-N-ethylcarbamoyl)-1,4-dihydrophenathrolin-4-one-3-carboxylic acid], with comparable potencies in vivo, were chosen to investigate the effect of the inhibition of the hydroxylation of newly synthesized uterine collagen on the turnover of this protein in vivo. (embl.de)
- l -3,4-dihydroxyphenylalanine , is an amino acid that is made and used as part of the normal biology of some plants [3] and animals, including humans. (cloudfront.net)
20191
- The coronavirus disease 2019 (COVID-19) is caused by the severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2). (bvsalud.org)
Reaction2
- Expressed sequence tag (EST) analysis and developmental polymerase chain reaction (PCR) studies demonstrated that Ov-phy-1 was expressed in L3 and adult parasites. (embl.de)
- In the reverse reaction, the 2'-hydroxyisoflavone (2'-hydroxydaidzein) is reduced to an isoflavanone. (qmul.ac.uk)
Enzymes2
- The two enzymes were identified to be a part of two separate catechol dioxygenase families: 1,2-CTD was classified as an intradiol dioxygenase while 2,3-CTD was classified as an extradiol dioxygenase. (wikipedia.org)
- Particularly, a Western blot of normal ws-PrP (ws-PrPC) and disease-associated ws-PrP (ws-PrPSc) [3] displayed a glycotyping that was different from that of the classical PrPC and PrPSc, showing a slightly faster migration mobility and a diglycoslated band with higher propensity to degradation by endogenous enzymes. (tam-receptor.com)
Bioremediation1
- Therefore, engineering of Catechol 2,3-dioxygenase may be employed for application on bioremediation of polluted sites. (openmicrobiologyjournal.com)
Protein11
- actin related protein 2/3 complex su. (gsea-msigdb.org)
- BCL2 interacting protein 1 [Source:H. (gsea-msigdb.org)
- death associated protein kinase 1 [S. (gsea-msigdb.org)
- Co-expression of Ov-phy-1 with the O. volvulus homologue of protein disulfide isomerase in a baculovirus system resulted in the production of enzymatically active O. volvulus prolyl 4-hydroxylase. (embl.de)
- Mouse monoclonal to NCOR1 water-soluble form of the prion protein (ws-PrP) in blood plasma and brain of Syrian hamster [3]. (tam-receptor.com)
- A correlation network between DFS-SEs of splicing factors and non-splicing factors was constructed to determine the potential mechanisms in PRAD, which included the potential regulatory interaction between the splicing event of splicing factor RNA binding motif protein 5-alternate terminator (AT)-64957 and the splicing event of non-splicing factor heterochromatin protein 1 binding protein 3-AT-939. (spandidos-publications.com)
- Panobinostat Parsaclisib depletes protein levels of DNMT1 and EZH2 and disrupts DNMT1 connection with EZH2 and the polycomb repressive complex 2 (PRC2) [50]. (edrc2013.org)
- Serum insulin-like growth factor (IGF)-I and IGF-binding protein-3 concentrations and prostate cancer risk: results from the European Prospective Investigation into Cancer and Nutrition. (mdc-berlin.de)
- Description: A sandwich quantitative ELISA assay kit for detection of Human AE Binding Protein 1 (AEBP1) in samples from tissue homogenates, cell lysates or other biological fluids. (kits-elisa.com)
- Description: A sandwich ELISA kit for detection of AE Binding Protein 1 from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids. (kits-elisa.com)
- Description: Quantitativesandwich ELISA kit for measuring Human Protein jagged-1 (JAG1) in samples from serum, plasma, tissue homogenates. (kits-elisa.com)
Mechanism1
- The catalytic mechanism of catechol 1,2-dioxygenase was elucidated using a combination of O18 labeling experiments and crystallography. (wikipedia.org)
Synthetase1
- alanyl-tRNA synthetase 1 [Source:HGN. (gsea-msigdb.org)
Wastewater2
- An aerobic bacterial strain C-14-1 was isolated from an acrylic fiber wastewater. (researchgate.net)
- Its application results in the production of large wastewater volumes which require treatment on site 2 . (nature.com)
Hydrocarbons2
- 1,2-CTD degrades phenolic hydrocarbons key to the synthesis lipid membranes. (wikipedia.org)
- Polycyclic aromatic hydrocarbons (PAHs) are one class of organic pollutants that are considered the most hazardous due to their toxic, mutagenic, and carcinogenic properties [ 1 ]. (omicsonline.org)
Degradation1
- Based on the complexity of the degradation pathways, the phenomenon of biodegradation is categorized into two types: convergent and divergent modes of degradation (Fig. 1 ). (biomedcentral.com)
Proteins1
- It is a complex of copper-containing proteins that acts also on a variety of substituted catechols. (lookformedical.com)
Acetyl1
- The 2,3-dihydrobenzofuran derivative, 7-acetyl-4,6-dimethoxy-2-isopropenyl-2,3-dihydrobenzofuran, had an ED 50 value of 1.3 μg (5.4×10 -9 mol)/cm 2 against the common cutworm. (go.jp)
Oxygen1
- Oxygenases can further be grouped into two categories i.e. monooxygenases and dioxygenases on the basis of number of oxygen atoms used for oxidation. (biomedcentral.com)
Compounds2
- Many of these compounds have been reported to be toxic to the living organisms [ 3 ]. (biomedcentral.com)
- A survey of agricultural technologies influencing the biosynthesis and accumulation of phenolic compounds in crop plants is presented, including observations on the effects of light, temperature, mineral nutrition, water management, grafting, elevated atmospheric CO 2 , growth and differentiation of the plant and application of elicitors, stimulating agents and plant activators. (mdpi.com)
Biology2
- Free Radical Biology and Medicine 42 (1): 32-43. (mdc-berlin.de)
- Journal of Structural Biology 159 (1): 158-163. (mdc-berlin.de)
Pyrene1
- Three of the set ups were supplemented each with 1 mg /L of xylene, anthracene and pyrene. (journalarrb.com)
20221
- 1867(2): 159070, 2022 02. (bvsalud.org)
Synthase1
- 5'-aminolevulinate synthase 1 [Sourc. (gsea-msigdb.org)
CDNA1
- A full-length cDNA (Ov-phy-1) encoding a catalytically active alpha-subunit of Onchocerca volvulus prolyl 4-hydroxylase was isolated and characterized. (embl.de)
Aldehyde1
- aldehyde dehydrogenase 1 family memb. (gsea-msigdb.org)
Reduction2
- this has seen reduction in SO 2 emissions across Europe by more than 60% between 1990 and 2004 (Vestreng et al. (springer.com)
- reproductive enthusiastic feed 's the Common reduction of 1,3-diaminobenzenes from entirely gradual risks. (xn--drpverein-rahe-vpb.de)
Specificity1
- The substrate specificity of catechol oxidase from Lycopus europaeus towards phenols is examined. (lookformedical.com)
Contaminants1
- Consequently, the World Health Organisation (WHO) and other environmental agencies have established guidelines for air pollution for a range of contaminants (Table 1 ). (springer.com)
Methyltransferase2
- catechol-O-methyltransferase [Source. (gsea-msigdb.org)
- DNA methyltransferase 3 like [Source. (gsea-msigdb.org)
Temperature1
- METHODS: Seawater samples were collected from Labrador Sea Water (LSW, ~ 2000 m depth) in the North Atlantic and incubated in the dark at in situ temperature unamended, amended with 1 µM thiosulfate, or with 1 µM thiosulfate plus 10 µM glucose and 10 µM acetate (thiosulfate plus dissolved organic matter, DOM). (bvsalud.org)
Microbiology2
- World journal of microbiology & biotechnology, 29 (1). (csircentral.net)
- Indian Journal of Microbiology, 56 (3). (csircentral.net)
Products1
- DNMTs are directly inhibited by DNA hypomethylating providers, while additional antitumor agents target additional GMD products [1, 7, 44C49]. (edrc2013.org)