• Sambrook was a senior staff scientist at Cold Spring Harbor Laboratory from 1969-1985. (google.com)
  • At noon every Tuesday from September through June, scenes from a revolution in neuroscience are playing out at Cold Spring Harbor Laboratory. (cshl.edu)
  • Cold Spring Harbor, NY - A new study of the genetic factors involved in the causation of autism spectrum disorders (ASD) draws fresh attention to the impact these illnesses have on motor skills, and more broadly on cognitive function. (cshl.edu)
  • Knowing that cancers become lethal when they spread, investigators at Cold Spring Harbor Laboratory (CSHL) seek a way of detecting tumors much earlier than now possible-when they're more likely to be curable. (cshl.edu)
  • Cold Spring Harbor, NY - The way you measure things has a lot to do with the value of the results you get. (cshl.edu)
  • Cold Spring Harbor, NY - Reducing levels of antioxidants in pancreatic cancer cells can help kill them, newly published research reveals, suggesting an entirely new treatment strategy for the notoriously lethal illness, in which less than 5 percent of patients survive 5 years. (eurekalert.org)
  • 1985) Comparative properties of five human ovarian adenocarcinoma cell lines. (mskcc.org)
  • Tumor endothelial cells (TECs) have been documented to demonstrate abnormal morphologic characteristics such as ragged margins and irregular cytoplasmic projections. (wikipedia.org)
  • In normal blood vessels, it is known that endothelial cells form regular monolayers with tight junctions without overlap, but TECs create disorganized and loosely connected monolayers, often branching and extending across the lumen to overlap with their neighbors. (wikipedia.org)
  • The tumor endothelium is often described as mosaic due to its aberrant expression of traditional endothelial cell markers (CD31 and CD105), supporting the existence of irregular gaps between endothelial cells. (wikipedia.org)
  • VEGF has been indicated to stimulate sprouting and tip branching in endothelial cells, leading to defective endothelial monolayers. (wikipedia.org)
  • Loss of pericyte growth factor (PDGFB) and its receptor on endothelial cells are molecular-level changes that can account for this abnormal loss in pericyte support. (wikipedia.org)
  • For many tumors, it has been discovered associated endothelial cells have significantly increased permeability. (wikipedia.org)
  • Sorafenib and sutinib are additional angiogenesis inhibitors that bind and block receptors on endothelial cells that have important roles in downstream pathways contributing to angiogenesis progression. (wikipedia.org)
  • Additionally, the evidence that dividing CD4 + T cells are infected more efficiently than resting T cells ( 55 ) implies that HIV infection, through inhibition of proliferation or cytotoxicity, would affect the in vivo generation of terminally differentiated CD4 + T cells ( 8 ). (asm.org)
  • TGFβs regulate many basic biological processes including cell proliferation, shape, adhesion, migration and differentiation. (els.net)
  • DISC1 expression was enriched in developing mouse and human pancreas and adult β- and ductal cells. (nih.gov)
  • Ductal cells are stained with CK19. (nih.gov)
  • C ) ISH of human pancreas showing high expression of DISC1 in islet (circled in red) and ductal cells (arrow) and low expression in exocrine pancreas. (nih.gov)
  • St. Jude investigators pioneered the use of haploidentical transplants, demonstrating that careful matching of patients and donors and proper processing of the hematopoietic donor cells enhances the anti-cancer effect of transplantation without significantly increasing side effects. (bio-medicine.org)
  • All-trans retinoic acid has been shown to suppress the growth of Caov-3 ovarian carcinoma cells in vitro . (mskcc.org)
  • They can be propagated in vitro for long periods of time in an undifferentiated state and thus represent an attractive source of cells for developmental studies and for therapy of human diseases. (google.com)
  • B ) Western blot with Ab3 or CM2B4 of cell lysates prepared from MCC cell lines showing a predominant signal for truncated large T antigen between 36 and 50 kDa. (jci.org)
  • D ) Western analysis of DISC1 expression in lysates from mouse β-cell lines (TC6 and MIN6) and human islets blotted with DISC1 antibody ± specific inhibitory peptide. (nih.gov)
  • On a larger scale, mitotic cell division can create progeny from multicellular organisms, such as plants that grow from cuttings. (wikipedia.org)
  • Mitotic cell division enables sexually reproducing organisms to develop from the one-celled zygote , which itself was produced by meiotic cell division from gametes . (wikipedia.org)
  • In this study we used polychromatic flow cytometry to sort multiple CD4 + and CD8 + T-cell subsets, defined by 11 phenotypic parameters, directly from the peripheral blood mononuclear cells (PBMC) of HIV-infected individuals. (asm.org)
  • Calambokidis J, Barlow J (1991) Chlorinated hydrocarbon concentrations and their use for describing population discreetness in harbor porpoises from Washington, Oregon, and California. (springer.com)
  • During G 2 , the cell undergoes the final stages of growth before it enters the M phase, where spindles are synthesized. (wikipedia.org)
  • However, there are now multiple studies that illustrate the complexity behind these previous simple conceptions of angiogenesis, indicating that the way cancer cells interact with and co-opt new blood vessel growth varies amongst cancer types and must be studied. (wikipedia.org)
  • Transforming growth factor beta (TGFβ) is the prototype for a family of multifunctional secreted peptides that control many aspects of growth and development in a cell‐type‐specific manner. (els.net)
  • G1 is a time of growth for the cell. (wikipedia.org)
  • During G2, the cell undergoes the final stages of growth before it enters the M phase. (wikipedia.org)
  • Historically, transplant patients fared best and suffered fewer complications when the donors were relatives who carried the same six proteins on their white blood cells. (bio-medicine.org)
  • Known as HLA proteins, they serve as markers to help the immune system distinguish between an individual's healthy tissue and diseased cells that should be eliminated. (bio-medicine.org)
  • Upon transfection into a human hepatoma cell line, these RNAs were found to replicate to high levels, permitting metabolic radiolabeling of viral RNA and proteins. (sciencemag.org)
  • These experiments provided a critical clue: The team saw that when NRF2 is missing, the machinery in cells that translates messages from genes into proteins - the process of protein synthesis -- is very sensitive to fluctuations in the balance between oxidants and anti-oxidants. (eurekalert.org)
  • The Johns Hopkins scientists reported in February that, in mice, the double dose of IGF-2 protein sets the stage for cancer development -- by increasing the number of primitive cells found in the colon -- and that early evidence suggests the same could be true in people. (bio-medicine.org)
  • It has been estimated that the frequency of CD4 + T-cell infection by HIV in vivo is probably too low to account alone for the death and dysfunction of T cells throughout the disease ( 5 , 17 ). (asm.org)
  • Previous studies that averaged the response across cell populations suggested that one or two DNA breaks are sufficient for activating ATM and p53. (biomedcentral.com)
  • While studies have demonstrated the presence of HIV in these T-cell subsets, the purity of T-cell populations analyzed ex vivo limits interpretations. (asm.org)
  • After the cell proceeds successfully through the M phase, it may then undergo cell division through cytokinesis. (wikipedia.org)
  • Developmental and homeostatic relationships between various T-cell compartments-thymocytes, naïve T cells, and resting memory, effector, and terminally differentiated T cells-are affected by HIV. (asm.org)
  • TGFβ inhibits the cell cycle by regulating the activity of cyclin dependent kinases. (els.net)
  • Cell division usually occurs as part of a larger cell cycle . (wikipedia.org)
  • Both of these cell division cycles are used in the process of sexual reproduction at some point in their life cycle. (wikipedia.org)
  • If the cell does not pass this checkpoint, then the cell will exit the cell cycle. (wikipedia.org)
  • We also show that the natural variations in the initial amount of p53, rate of DSB repair and cell cycle phase do not affect the probability of activating p53 in response to DNA damage. (biomedcentral.com)
  • Additionally, the present invention is directed to a method of enhancing the survival of the differentiated neural cells in long term cell cultures. (google.com)
  • The largest survival gains involved patients whose blood and immune systems were rebuilt with cells from haploidentical donors. (bio-medicine.org)
  • I aim to discover novel cancer therapeutics through both the development of novel molecular target cell-based assays and the determination of the mechanism of action of compounds discovered at HBOI. (fau.edu)
  • B-cell lymphoma, a common morphologic variant of non-Hodgkin lymphoma, has been associated with persistent pollutants in humans, but this association is not well-characterized in top-level predators sharing marine resources with humans. (springer.com)
  • Here, we used polychromatic flow cytometry to sort multiple T-cell subsets to 99.8% purity, followed by quantitative PCR to quantify HIV gag DNA directly ex vivo. (asm.org)
  • There are limited data on HIV infection of memory and naïve CD4 + T cells in vivo ( 10 , 42 ) and CD8 + T cells ( 25 , 30 , 34 , 44 ). (asm.org)
  • These data illuminate the underlying mechanisms that distort T-cell homeostasis in HIV infection. (asm.org)
  • Although human immunodeficiency virus type 1 (HIV-1) infection is characterized by the progressive depletion of CD4 + T cells, the mechanisms underlying this remain controversial ( 18 , 22 ). (asm.org)
  • TGFβ is secreted from cells in a latent, inactive form that is activated through proteolytic and nonproteolytic mechanisms. (els.net)
  • These cells form tightly packed colonies in adherent culture. (mskcc.org)
  • Within cells, levels of p53 protein are tightly controlled by several regulatory feedback loops that direct its stability and degradation. (biomedcentral.com)
  • This family of molecules is useful in identifying and/or quantifying TGF-β1 in a sample, as well as inhibiting its effect on cells. (google.com)
  • As a positive control, PCR was performed with 10 7 plasmid molecules or 1 μg of DNA from a BHK cell line stably transfected with the neo gene (lanes 1 and 2), and as a negative control PCR was performed without a DNA template (lane 13). (sciencemag.org)
  • For simple unicellular microorganisms such as the amoeba , one cell division is equivalent to reproduction - an entire new organism is created. (wikipedia.org)
  • On the other hand, embryonic stem (ES) cells are pluripotent cells that are both self-renewing and have the capacity to differentiate into any cell type in the human organism. (google.com)
  • The use of fluorescent reporters to quantify DNA damage and p53 levels in live cells provided a quantitative analysis of the complex interrelationships between both processes. (biomedcentral.com)
  • ES cells are responsive to environmental cues upon transplantation and adopt a cellular fate that is appropriate to the transplanted region. (google.com)
  • This work defines the structure of HCV replicons functional in cell culture and provides the basis for a long-sought cellular system that should allow detailed molecular studies of HCV and the development of antiviral drugs. (sciencemag.org)
  • It involves collaborative clinical studies to discover mutational patterns predicting treatment response and outcome and the development of diagnostics to detect cancer cells in bodily fluids such as blood and urine. (cshl.edu)
  • We present evidence that the decision to activate p53 given a specific number of breaks is not entirely stochastic, but instead is influenced by both cell-intrinsic factors and previous exposure to DNA damage. (biomedcentral.com)
  • Prokaryotes ( bacteria ) undergo a vegetative cell division known as binary fission , where their genetic material is segregated equally into two daughter cells. (wikipedia.org)
  • Understanding the origin and consequences of such variability in normal and cancerous cells is crucial for developing efficient and selective therapeutic interventions. (biomedcentral.com)
  • These results uncover an unexpected role for DISC1 in normal β-cell physiology and suggest that DISC1 dysregulation contributes to T2D independently of its importance for cognition. (nih.gov)
  • The Caov-3 cells harbor a nonsense mutation in the p53 gene, and have multiple copies of the ovarian cancer oncogene PIK3CA . (mskcc.org)
  • in Cancer Cells 3 (Feramisco et al. (google.com)
  • I lead the Cancer Cell Biology Group of the Marine Biomedical and Biotechnology Research Program at Harbor Branch Oceanographic Institute (HBOI). (fau.edu)
  • Of course, that's exactly what we want cancer cells to do - to burn themselves out," observes Iok In Christine Chio, a postdoctoral investigator in the Tuveson lab who led experiments reported today in the journal Cell . (eurekalert.org)
  • but when it comes to killing cancer cells, they are anathema. (eurekalert.org)
  • One way of increasing oxidation in cancer cells is to decrease levels of antioxidants in those same cells. (eurekalert.org)
  • Scientists call it a master regulator of redox homeostasis - by which they mean that it's one of the switches one can tweak to disturb the exquisite balance between oxidation and reduction in cancer cells. (eurekalert.org)
  • Oxidizing and anti-oxidizing agents are created in every cell, and are kept in a very precise balance when cells are healthy. (eurekalert.org)