• Interleukin-12 (IL-12) and interleukin-23 (IL-23), which belong to the IL-12 family of cytokines, have a key role in intestinal homeostasis and inflammation and are implicated in the pathogenesis of inflammatory bowel disease. (nature.com)
  • This Review provides a summary of the biology of the IL-12 family cytokines IL-12 and IL-23, discusses the role of these cytokines in intestinal homeostasis and inflammation, and highlights IL-12- and IL-23-directed drug development for the treatment of Crohn's disease and ulcerative colitis. (nature.com)
  • IL-12 and IL-23, which are members of the IL-12 family of cytokines, have a key role in intestinal homeostasis and inflammation, including in inflammatory bowel disease. (nature.com)
  • TYK2 dimerises with JAK2 to mediate IL-12 and IL-23 signalling (both are p40 subunit containing cytokines), and with JAK1 for the IFNα/β pathway. (guidetopharmacology.org)
  • Ustekinumab is a new, human monoclonal antibody with a novel mechanism of action that targets the p40 sub-unit of cytokines interleukin-12 (IL-12) and interleukin-23 (IL-23), naturally occurring proteins that are important in regulating immune responses and that are thought to be associated with some immune-mediated inflammatory disorders, including plaque psoriasis. (pipelinereview.com)
  • The assay is compatible with human samples only, and is highly specific for p40, cross-reacting with all p40-containing cytokines such as IL12, IL23, p40 monomer, and p40-p40 (p80) dimer. (cisbio.net)
  • p40 is a common component of the p40 family of cytokines which encompasses IL-12 (p40+p35 heterodimer), IL-23 (p40+p19 heterodimer), p40 (monomer), and p80 homodimer (p40+p40). (cisbio.net)
  • It combines with INTERLEUKIN-12 SUBUNIT P40, which is shared between the two cytokines, to form in the active interleukin-23 cytokine. (jefferson.edu)
  • Binding of IL-23 to its receptor on macrophages promotes secretion of additional proinflammatory cytokines, including TNF-alpha and interleukin-1 beta (IL-1β). (lillymedical.com)
  • Both IL-12 and IL-23 are cytokines belonged to the IL-12 family, which plays important roles in shaping immune responses during antigen presentation and influence cell-fate decisions of differentiating naïve T cells, and regulating functions of a variety of effector cells. (creativebiolabs.net)
  • It was found that IL-23 prolongs the expression of type 17 signature cytokines (such as IL-17, IL-22 and GM-CSF) that induce tissue pathology and mediates chronic inflammation by promoting the survival and maintenance of Type 17 cells. (creativebiolabs.net)
  • These agents inhibit pro-inflammatory cytokines like interleukins (ILs), especially IL-12 and IL-23. (jennikwondesigns.com)
  • Ustekinumab is normally a fully individual immunoglobulin G1k monoclonal antibody that particularly blocks the distributed p40 subunit Galanin (1-30) (human) of IL-12 and IL-23 [3], taking place regulatory cytokines involved with inflammatory and immune system replies normally, organic killer cell activation, and signaling for downstream effector cytokine creation (e.g. (euromed2016.com)
  • Subunit beta of interleukin 12 (also known as IL-12B, natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor p40, or interleukin-12 subunit p40) is a protein subunit that in humans is encoded by the IL12B gene. (wikipedia.org)
  • Interleukin 12 is a disulfide-linked heterodimer composed of the 40 kDa cytokine receptor like subunit encoded by this gene, and a 35 kDa subunit encoded by IL12A. (wikipedia.org)
  • The C15.6 antibody reacts with mouse IL-12 p40 subunit of the IL-12 p70 and IL-23 p40 subunit of the IL-23 p19/p40, as well as p40 monomer and homodimer, or heterodimer. (biolegend.com)
  • This assay detects p40 as monomer, homodimer, or heterodimer complexed with p35. (biolegend.com)
  • IL-23 is a complexed heterodimer of the IL-23A (19 subunit) and IL-12B (p40 subunit). (abbkine.com)
  • The ab221837 pair recognize the heterodimer by using antibodies to both the p19 and p40 subunits. (abbkine.com)
  • Disulfide-bonded heterodimer of 40 kda and 35 kda subunits. (lu.se)
  • Apparent molecular mass of 60 kDa in SDS-PAGE under non-reducing conditions, 41 kDa and 29 kDa under reducing conditions, a disulfide-linked heterodimer consisting of the 306 amino acid rHuIL-12 p40 and the 197 amino acid rHuIL-12 p35 subunits. (watson-bio.com)
  • IL-12 beta Protein, also known as natural killer cell stimulatory factor 2, is a common subunit (p40) of IL-12 and IL-23. (medchemexpress.com)
  • IL-23 is secreted by activated dendritic cells and macrophages, and signals though a receptor comprised of IL-23R complexed with IL-12Rβ1. (peprotech.com)
  • Both homodimeric p40, and IL-23 can bind to the IL-12 receptor, serving as non-signaling antagonists. (rndsystems.com)
  • IL-23 signals through a receptor complex consisting of IL-12 R beta 1 and IL-23 R. This complex is expressed in mouse Th1 and Th2 cells, bone marrow dendritic cells, activated macrophages and CD4+ CD45Rb(low) memory T cells. (rndsystems.com)
  • Both possess a common p40 subunit but IL-23 has a unique p19 protein subunit that allows it to bind the IL-23 receptor. (dermatologytimes.com)
  • composed of the p40 and IL-12-specific p35 subunits.66 IL-23 p40/p19 binds to the IL-23 receptor, which comprises IL-23R and IL-12RB1. (bioinf.org)
  • This up-regulates IL-23R (which is not present on naive CD4+ cells), sensitising the cells to IL-23, and causes cytokine production by RAR-related orphan receptor ?t (ROR?t) expression. (thenewstandardgallery.com)
  • IL-12 p40 subunit plasmatic level was decreased, but the IL-12 receptor was present and functional. (cdc.gov)
  • The p40 is also part of IL-12 and binds to the IL-12 receptor beta 1 (IL-12Rβ1) subunit. (rowan.edu)
  • Thus, it follows that the IL-23 receptor is comprised of the IL-12Rβ1 and IL-23 receptor alpha (IL23Rα) subunits. (rowan.edu)
  • New research in therapeutics for autoimmune disease is attempting to inhibit IL-23 from binding to its receptor. (rowan.edu)
  • IL-23 is also a heterodimeric cytokine composed of an IL12B (IL-12p40) subunit and the IL23A (IL-23p19) subunit, and its high-affinity receptor is IL-23 receptor (IL-12Rβ1/IL-23R). (creativebiolabs.net)
  • As an inhibitor, it can bind to the p40 subunit of IL-12 and IL-23, blocking its binding at the receptor and the subsequent downstream signaling. (creativebiolabs.net)
  • Ustekinumab (UST) is a monoclonal antibody targeting IL-12 and IL-23 through their shared p40 subunit. (ecco-ibd.eu)
  • Centocor reported one year data from a phase III trial of ustekinumab (CNTO-1275), a monoclonal antibody against the p40 subunit of IL-12 and IL-23 for the treatment of moderate to severe plaque psoriasis. (centerwatch.com)
  • Ustekinumab is the first anti-IL drug to be developed or approved for IBD and blocks both IL-12 and IL-23 through their p40 subunit. (jennikwondesigns.com)
  • The HTRF Human IL12/23 p40 kit is designed for the quantification of human IL12/23 p40 release in cell supernatant. (cisbio.net)
  • By clicking on this link, you'll be able to access a free online software to run your IL12/23 p40 analysis. (cisbio.net)
  • The IL12/23 p40 standard curve is generated in the assay Diluent 5 provided in the kit. (cisbio.net)
  • Briakinumab recognizes both human IL12 and IL23 via IL-12/23p40. (abeomics.com)
  • IL-12 beta Protein, as known as IL12 p40 subunit or IL-12B, heterodimerizes with the IL-12 p35 subunit (IL-12A) to form IL-12 and with the IL23 p19 subunit to form IL-23, exerting different regulating functions [1] . (medchemexpress.com)
  • In humans, the p40 subunit can also associate with a p19 subunit to form IL-23. (watson-bio.com)
  • IL-23 is a proinflammatory, heterodimeric protein composed of two subunits: a unique p19 subunit, and a p40 subunit that is shared with IL-12. (peprotech.com)
  • Recombinant Human IL-23 is a 53.5 kDa, heterodimeric protein consisting of the two subunits, p19 (170 amino acids) and p40 (306 amino acids). (peprotech.com)
  • ii] Gene expression analysis of the IL-12 cytokine family subunits revealed that both strains induced high levels of p40(protein chain communal to IL-12 p70 and IL-23) as well as p19, a subunit of IL-23. (rockland.com)
  • IL-23 is a heterodimeric protein, composed of a p19 alpha chain and a p40 beta chain. (rowan.edu)
  • This work generated a Peptide #2-Fc fusion protein with the capability to bind the IL23Rα chain and thus block IL-23 binding and inhibit subsequent signaling, as well an increased serum half-life over the original Peptide #2. (rowan.edu)
  • For this purpose, p40 protein concentrations in cerebrospinal fluid specimens from diseased patients (n = 39) were compared to those from individuals without Alzheimer's disease (n = 20). (paasp.net)
  • however, instead of 59 data points as expected, Figure S9 only showed n=6 (control) and n=7 (AD) measurements, respectively, indicating that most individuals could not be included in this study - the p40 protein concentrations were simply below detection limit. (paasp.net)
  • IL-23 (Interleukin-23) is a disulfide-linked cytokine composed of a p19 subunit that is unique to IL-23 and a p40 subunit that is shared with IL-12. (rndsystems.com)
  • Interleukin(IL)-12 is a 70-KDa cytokine comprised of two disulfide-linked proteins(p35 and p40) and is essential for the initiation of effective immune response. (rockland.com)
  • IL-12 is a heterodimeric cytokine of disulfide-linked p35 and p40 subunits. (watson-bio.com)
  • IL-23 induces the earliest recruitment of neutrophils to the site of infection and promotes the development and maintenance of Th17 cells. (rndsystems.com)
  • Dr. KruegerDermatologists' appreciation of the central role that the interleukin (IL)-23/Th17 pathway plays in psoriasis has developed gradually, through research and serendipity, according to James Krueger, M.D., Ph.D., who spoke on the topic at the MauiDerm 2017 meeting. (dermatologytimes.com)
  • To maintain the population of TH17, IL-23 is required, otherwise the cells dissipate[1,2,4,6,8].The final type of CD4+ cell to be considered is the CD4+CD25+ Treg cell, and this is due to the close relationship between TH17 and induced Treg (iTreg) cells. (thenewstandardgallery.com)
  • Th17 cells are functionally dependent on the myeloid-derived cytokine IL-23. (baxkyardgardener.com)
  • The cytokine interleukin-23 (IL-23) plays an essential role in the expansion of pathogenic Th17 cells. (rowan.edu)
  • IL-23 induces auto immune inflammation and may be responsible for auto immune inflammatory diseases. (abbkine.com)
  • Briakinumab is a human monoclonal antibody targets the p40 subunit shared by interleukins 12 and 23. (abeomics.com)
  • Open up in another window Launch Although natural therapies such as for example antagonists of tumor necrosis aspect (TNF)- or interleukins (IL)-12/23 generally give improved efficiency over typical immunosuppressants (IMMs) in dealing with immune-mediated inflammatory disorders, theoretical dangers connected with impaired immune system surveillance stay under research. (euromed2016.com)
  • Briakinumab is a fully human monoclonal antibody directed against the p40 subunit shared by IL-12 and IL-23, two inflammation factors associated with autoimmune disease. (creativebiolabs.net)
  • IL-12B is a common subunit of interleukin 12 and interleukin 23. (wikipedia.org)
  • Interleukin (IL)-23, a heterodimeric cytokine, is composed of an IL-23-specific subunit p19 and an IL-12-common subunit p40 [ 10 ]. (oncotarget.com)
  • Briakinumab is designed as a monoclonal antibody targets and neutralizes IL-12 and IL-23. (creativebiolabs.net)
  • It contains Sf 21-expressed recombinant mouse IL-23 and antibodies raised against the recombinant p19 and p40 subunits. (rndsystems.com)
  • Multiple IL-12- and/or IL-23-neutralizing antibodies have been tested in immune-mediated diseases, including Crohn's disease and ulcerative colitis. (nature.com)
  • EliKine™ Human IL-23 ELISA Kit has high sensitivity and excellent specificity for detection of Human IL-23. (abbkine.com)
  • Cells were then stained with 0.25 µg of APC-conjugated rat anti-mouse IL-12 (p40/p70) antibody (APC-C15.6, Cat. (bdbiosciences.com)
  • Anti-Human IL 12/23 (Briakinumab) utilizes the same variable regions from the therapeutic antibody Briakinumab making it ideal for research projects. (abeomics.com)
  • An antibody specific for IL-23 has been pre-coated onto a microplate. (abbkine.com)
  • Standards and samples are pipetted into the wells and any IL-23 present is bound by the immobilized antibody. (abbkine.com)
  • After removing any unbound substances, a biotin-conjugated antibody specific for IL-23 is added to the wells. (abbkine.com)
  • Briakinumab, also known as ABT-874, is a therapeutic antibody targeting at interleukin-12 (IL-12) and interleukin-23 (IL-23). (creativebiolabs.net)
  • According to the conclusion stated in the article, intracerebroventricular delivery of a neutralizing p40-specific antibody (p40 is the common IL-12 and IL-23 subunit) reversed cognitive deficits in aged APPPS1 mice ("Alzheimer" mouse). (paasp.net)
  • This gene encodes a subunit of interleukin 12, a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. (wikipedia.org)
  • Interleukin-12 p40 also serves as a subunit of interleukin 23. (wikipedia.org)
  • IL-12 (Interleukin-12) is a heterodimeric cytokine that consists of a p35 and a p40 subunit. (rndsystems.com)
  • Interleukin-12 (IL-12) is a heterodimeric molecule composed of p35 and p40 subunits. (hoxyl.pl)
  • Inhibition of the p40 subunit also inhibits interleukin-12 (IL-12) signaling because the p40 subunit is shared with IL-12. (lillymedical.com)
  • Interleukin-23 is a heterodimeric cytokine made up of a p19 and a p40 subunit, and this provides different options for inhibition. (lillymedical.com)
  • 2012) Inhibition of IL-12/IL-23 signaling reduces Alzheimer's disease-like pathology and cognitive decline . (paasp.net)
  • No significant cross-reactivity or interference between Human IL-23 and analogues was observed. (abbkine.com)
  • This assay does not recognize free p19 or p40 subunits. (rndsystems.com)
  • The recovery of mouse IL-23 spiked to three levels throughout the range of the assay was evaluated. (rndsystems.com)
  • To assess the linearity of the assay, samples spiked with high concentrations of mouse IL-23 in each matrix were diluted with Calibrator Diluent and assayed. (rndsystems.com)
  • Risankizumab is an IL-23 inhibitor newly approved in the United States and United Kingdom for moderate-to-severe CD. (jennikwondesigns.com)
  • Some were highly inflammatory secreted molecules including S100A12, IL-20 and most importantly IL-23. (dermatologytimes.com)
  • Although IL-12 was predominantly investigated before the discovery of IL-23, many recent studies using IL-23p19 -/- mice have identified that IL-23 but not IL-12 has a key role in orchestrating an inflammatory cytokine cascade involving enhanced expressed levels of IL-17, TNFα, IFNγ and IL-6 in the intestine [ 11 - 14 ]. (oncotarget.com)
  • IL-23 is associated with various autoimmune inflammatory diseases, and is particularly highly expressed in psoriasis skin lesions. (abeomics.com)
  • Briakinumab binds to and neutralizes human IL-12 and IL-23 (via their shared p40 subunit) and is being investigated for the treatment of rheumatoid arthritis, inflammatory bowel disease, and multiple sclerosis. (abeomics.com)
  • IL-23 deficiency also severely restricts the development of experimentally induced tumors accompanied by a deficiency of inflammatory mediators such as IL-17, tumor-promoting inflammatory metalloproteases and inflammation driven angiogenesis [40,41]. (baxkyardgardener.com)
  • The pro-inflammatory IL-23 also suppressed NK cell mediated tumor rejection [40]. (baxkyardgardener.com)
  • Gran B, Zhang GX, Rostami A. Role of the IL-12/IL-23 system in the regulation of T-cell responses in central nervous system inflammatory demyelination. (jefferson.edu)
  • IL-23 and autoimmunity: new insights into the pathogenesis of inflammatory bowel disease. (lillymedical.com)
  • Il-23 was found in a variety of pathological tissues such as the skin of patients with psoriasis, in the bowel wall of patients with chronic inflammatory bowel disease, and in synovial membrane of patients with rheumatoid arthritis. (creativebiolabs.net)
  • For testing mouse IL-12 p40 (monomer, dimer, heteromer) in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. (biolegend.com)
  • On December 23, 2022, Oncotarget server experienced a DDoS attack. (oncotarget.com)
  • Peptides #7 and #2 were identified as having the strongest inhibitory activity on the IL-23 signaling pathway. (rowan.edu)
  • IL-10 activates STAT3 which inhibits expression from the distributed p40 subunit of IL-23 and IL-12. (baxkyardgardener.com)
  • Genetic or therapeutic ablation of IL-23 in mice renders them resistant to experimentally induced autoinflammatory diseases [39]. (baxkyardgardener.com)
  • In addition, Supplementary Figure 9 may be worth a look as well: here, CNS p40 concentrations were analyzed in human samples to see whether previous findings in mice could be translated to humans with Alzheimer's disease. (paasp.net)
  • We were the first group to identify that IL-12 p35 and p40 subunits were differentially regulated by interferon regulatory factor-1 (IRF-1), namely IRF-1 mainly regulated IL-12 p35 gene transcription but not the p40 gene. (vasculitisfoundation.org)
  • IL-23 acts as a growth factor for activated T and NK cells. (abbkine.com)
  • IL-23 has been shown to enhance proliferation of memory T cells. (peprotech.com)
  • Determined by its ability to stimulate the proliferation of HEK-Blue™ IL-23 reporter cells. (peprotech.com)
  • The important role of interleukin-23 (IL-23) in the pathophysiology of IBD and the relevance of targeting IL-23 have been established via genetic studies, functional assessment in murine models, and studies using patient cells. (lillymedical.com)
  • This information focuses on the IL-23 axis only and does not preclude involvement of other immune factors within the pathophysiology of IBD. (lillymedical.com)
  • Fig. 2: Cellular sources, target cells, signalling and downstream effects of IL-23. (nature.com)
  • It is composed of EBI3 (EBV-induced gene 3), a 34 kDa glycoprotein that is related to the p40 subunit of IL-12 and IL-23, and p28, the 28 kDa glycoprotein that is related to the p35 chain of IL-12). (creativebiomart.net)
  • Li J, Gran B, Zhang GX, Ventura ES, Siglienti I, Rostami A, Kamoun M. Differential expression and regulation of IL-23 and IL-12 subunits and receptors in adult mouse microglia. (jefferson.edu)
  • This immunoassay has been shown to accurately quantitate the recombinant mouse IL-23. (rndsystems.com)
  • EliKine™ Human IL-23 ELISA Kit employs a two-site sandwich ELISA to quantitate IL-23 in samples. (abbkine.com)
  • The Quantikine Mouse IL-23 immunoassay is a 4.5 hour solid-phase ELISA designed to measure IL-23 in cell culture supernates, mouse serum, and plasma. (rndsystems.com)