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  • agarose
  • Agarose gels don't suffer from this limitation, because they aren't produced through exposure to UV light, but they aren't very good at separating small DNA strands and so tend to be restricted to longer strands. (separationsnow.com)
  • Next, the chemists compared their click gel with an agarose gel, which can also incorporate fluorescent compounds but isn't very good separating small DNA strands. (separationsnow.com)
  • protein
  • By comparing the relative ratio of the distance traveled by each protein to the length of the gel (Rf) one can make conclusions about the relative molecular weight of the proteins, where the length of the gel is determined by the distance traveled by a small molecule like a tracking dye. (wikipedia.org)
  • Affinity chromatography is a method of separating biochemical mixtures based on a highly specific interaction between antigen and antibody, enzyme and substrate, receptor and ligand, or protein and nucleic acid. (wikipedia.org)
  • Affinity chromatography is an excellent choice for the first step in purifying a protein or nucleic acid from a crude mixture. (wikipedia.org)
  • If the molecular weight, hydrophobicity, charge, etc. of a protein is unknown, affinity chromatography can still apply to this situation. (wikipedia.org)
  • selectivity
  • As hoped, the click gel performed much better than the polyacrylamide gel, able to separate the DNA ladder into clear bands with significantly improved efficiency and selectivity and much less band broadening. (separationsnow.com)
  • The high selectivity of affinity chromatography is caused by allowing the desired molecule to interact with the stationary phase and be bound within the column in order to be separated from the undesired material which will not interact and elute first. (wikipedia.org)
  • regions
  • This is because certain regions of the acrylamide solution often receive more illumination than others, producing a variation in the viscosity of the gel that can cause band broadening. (separationsnow.com)
  • chemists
  • This produces a classic click chemistry reaction, in which the copper snaps together the alkyne and azido groups on each monomer, and so the chemists refer to the resultant polymer hydrogel as a click gel. (separationsnow.com)
  • composition
  • Both by weight and volume, gels are mostly fluid in composition and thus exhibit densities similar to those of their constituent liquids. (wikipedia.org)
  • stationary phase
  • The sol-gel process facilitated the fabrication of a porous silica glass layer with the stationary phase in one single step. (springer.com)
  • In summary, affinity chromatography exploits the differences in interactions' strengths between the different biomolecules within a mobile phase, and the stationary phase. (wikipedia.org)
  • Analysis
  • The coupling of chromatography with MS is a well developed chemical analysis strategy dating back from the 1950s. (wikipedia.org)
  • Blue Devil Film is used for blotting, sequencing, chemiluminescent autoradiography (ecl) and gel shift analysis. (wikipedia.org)
  • Blue Devil provides film is used for autoradiography, Western blotting, sequencing, chemiluminescence and gel shift analysis. (wikipedia.org)
  • molecules
  • Large molecules get trapped at the beginning of the concentrated gel since they are too big to enter the pores of the gel. (wikipedia.org)
  • Performance
  • In addition to being able to incorporate the fluorescent compounds, another reason for the click gel's enhanced performance was that heating ensured a more uniform gel than exposure to UV light. (separationsnow.com)
  • 2017 - Launches GenClone product line, a full line of cell culture media products centered around ultra-pure, high-performance fetal bovine sera. (wikipedia.org)
  • polymerization
  • Because this polymerization process doesn't require UV light, fluorescent compounds can be incorporated into the click gel. (separationsnow.com)
  • As with polyacrylamide gels, the click gels are added into the channels as a solution, with the polymerization process taking just 10 minutes if the gels are heated to 70 ° C. (separationsnow.com)
  • system
  • Overall, the interface is a mechanically simple part of the LC-MS system that transfers the maximum amount of analyte, removes a significant portion of the mobile phase used in LC and preserves the chemical identity of the chromatography products (chemically inert). (wikipedia.org)
  • Gels are defined as a substantially dilute cross-linked system, which exhibits no flow when in the steady-state. (wikipedia.org)
  • polymers
  • Disposable diapers where they absorb urine, or in sanitary napkins Contact lenses (silicone hydrogels, polyacrylamides, polymacon) EEG and ECG medical electrodes using hydrogels composed of cross-linked polymers (polyethylene oxide, polyAMPS and polyvinylpyrrolidone) Water gel explosives Rectal drug delivery and diagnosis Encapsulation of quantum dots Breast implants Glue Granules for holding soil moisture in arid areas Dressings for healing of burn or other hard-to-heal wounds. (wikipedia.org)
  • stronger
  • The high surface area of the coating, characteristic of materials fabricated by the sol-gel process, resulted in capillary columns with a stronger retention than those prepared by a conventional procedure. (springer.com)
  • compounds
  • This prevents fluorescent compounds that emit light when they bind to DNA from being incorporated into the gel, because the UV light tends to deactivate them. (separationsnow.com)
  • At the moment, then, there's no effective way to separate small DNA strands in a gel containing fluorescent compounds. (separationsnow.com)
  • Affinity chromatography can be used to purify and concentrate a substance from a mixture into a buffering solution, reduce the amount of unwanted substances in a mixture, identify the biological compounds binding to a particular substance, purify and concentrate an enzyme solution. (wikipedia.org)
  • process
  • An organically modified silica glass material containing octyl groups (C 8 ) was fabricated by the sol-gel process and coated onto the inner walls of fused silica capillaries. (springer.com)
  • processes
  • Other synthetic routes include: controlled sol-gel processes, group transfer polymerization, transition metal catalysis, living anionic polymerization, living cationic polymerization, ring opening polymerization, ring-opening metathesis polymerization (ROMP), and controlled radical polymerization. (wikipedia.org)
  • High
  • High performance liquid chromatography (HPLC) is used to measure the amount of capsaicin and major capsaicinoids within pepper sprays. (wikipedia.org)
  • The high selectivity of affinity chromatography is caused by allowing the desired molecule to interact with the stationary phase and be bound within the column in order to be separated from the undesired material which will not interact and elute first. (wikipedia.org)
  • Uses
  • Formulated with collagen and wound dressing matrix, one product that uses chondroitin sulfate is the veterinary wound gel Chondroprotec, which is applied over scrapes, burns, and lesions and serves to keep the wound moist and promote healing. (wikipedia.org)