• 2006. Microbial dioxygenase gene population shifts during polycyclic aromatic hydrocarbon biodegradation. (nih.gov)
  • Moreover, a dioxygenase gene, nidA3B3, was detected in Mycobacterium vanbaalenii PYR-1 as an alternate degradation pathway which could catalyze both the initial dihydroxylation of pyrene [ 11 ] to be pyrene cis-4,5-dihydrodiol, and an alternate detoxification pyrene pathway to be pyrene cis-1,2-dihydrodiol [ 12 ]. (omicsonline.org)
  • Each enzyme entry contains its common name and synonym, reaction in which enzyme is involved, family and subfamily, structure and gene link and literature citation. (biomedcentral.com)
  • Catechol 2,3-dioxygenase (C23O) gene was found andamplified with the designed primers from the total DNA of C-14-1. (researchgate.net)
  • Oxygenases belong to the oxidoreductive group of enzymes (E.C. Class 1), which oxidize the substrates by transferring oxygen from molecular oxygen (O 2 ) and utilize FAD/NADH/NADPH as the co-substrate. (biomedcentral.com)
  • Oxygenases can further be grouped into two categories i.e. monooxygenases and dioxygenases on the basis of number of oxygen atoms used for oxidation. (biomedcentral.com)
  • At present the database contains information of over 235 oxygenases including both dioxygenases and monooxygenases. (biomedcentral.com)
  • Peripheral enzymes, particularly oxygenases and dehydrogenases, were found to transform structurally diverse substrates into one of these central intermediates by bringing about the hydroxylation of the aromatic nucleus (Fig. 2A ), and hence it is thought that bacteria have developed these enzymes to extend their substrate range [ 5 ]. (biomedcentral.com)
  • PAHs could be degraded by bacteria under aerobic conditions through the initial oxidation of the aromatic ring, which is catalyzed by the dioxygenase enzyme. (omicsonline.org)
  • A number of bacteria that can degrade a variety of aromatic compounds have been identified and the pathways involved in the degradation have been extensively characterized [ 3 , 4 ]. (biomedcentral.com)
  • Identification of metabolites from phenanthrene oxidation by phenoloxidases and dioxygenases of Polyporus sp. (afpm.org.my)
  • An enzyme that catalyzes the oxidation of catechol to muconic acid with the use of Fe3+ as a cofactor. (nih.gov)
  • The metal ligands and active site residues of extradiol dioxygenases are conserved although several amino acid residues found exclusively in enzymes which preferentially cleave bicyclic substrates are missing in BphC_JF8. (muni.cz)
  • In the convergent mode, structurally diverse aromatic compounds are converted to one of a few aromatic ring cleavage substrates such as catechol, gent sate, protocatechuate and their derivatives [ 5 ]. (biomedcentral.com)
  • ICP-MS analysis confirmed the presence of 4.0 to 4.8 manganese atoms per enzyme molecule. (muni.cz)
  • Non-heme iron-containing enzymes that incorporate two atoms of OXYGEN into the substrate. (nih.gov)
  • New metabolic pathways of phenanthrene and a better understanding of the phenoloxidases and dioxygenase mechanism involved in degradation of phenanthrene were explored in this research. (afpm.org.my)
  • Further, the centralized degradation pathways mean synthesis of fewer degradative enzymes requiring less metabolic energy. (biomedcentral.com)
  • A novel thermostable Mn(II)-dependent 2,3-dihydroxybiphenyl 1,2-dioxygenase (BphC_JF8) catalyzing the meta-cleavage of the hydroxylated biphenyl ring was purified from the thermophilic biphenyl and naphthalene degrader, Bacillus sp. (muni.cz)
  • Dehydrogenation of phenanthrene-cis-9,10-dihydrodiol to produce the corresponding diol, followed by ortho-cleavage of the oxygenated ring, produced 2,2'-diphenic acid. (afpm.org.my)
  • In this divergent mode, a metal-dependent dioxygenase channels these dihydroxylated intermediates into one of the two possible pathways: the meta -cleavage pathway or the ortho -cleavage pathway [ 7 - 9 ] (Fig. 1 ). (biomedcentral.com)
  • Cloning and characterization of a novel thermostable Mn(II)-dependent 2,3-dihydroxybiphenyl 1,2-dioxygenase from a PCB and naphthalene degrading Bacillus sp. (muni.cz)
  • 2003. Characterization of a novel Acinetobacter species containing a toluene dioxygenase. (nih.gov)
  • in the degradative pathways of 2-aminobenzenesulphonic, benzenesulphonic and 4-toluenesulphonic acids in Alcaligenes sp. (wikipedia.org)
  • The enzyme is resistant to denaturation by various chelators and inhibitors (EDTA, 1,10-phenanthroline, H2O2, 3-chlorocatechol) and did not exhibit substrate inhibition even at 3 mM 2,3-dihydroxybiphenyl. (muni.cz)
  • A 3-D homology model of BphC_JF8 provided a basis for understanding the substrate specificity, quaternary structure and stability of the enzyme. (muni.cz)
  • The enzyme has a molecular mass of 125+-10 kDa and was composed of 4 identical subunits (35 kDa). (muni.cz)
  • low-molecular-weight (LMW) compounds consist of two or three rings, and high-molecular-weight (HMW) compounds, more than three rings [ 3 ]. (omicsonline.org)
  • A decrease in Km accompanied an increase in temperature and the Km value of 0.095 uM for 2,3-dihydroxybiphenyl (at 60 C) is among the lowest reported. (muni.cz)
  • Specific activities of Catechol-2,3-dioxygenase and effects of temperature and pH and their stabilities on the enzyme relative activities were observed. (openmicrobiologyjournal.com)
  • Many of these compounds have been reported to be toxic to the living organisms [ 3 ]. (biomedcentral.com)
  • Oxygenation and spontaneous deamination of 2-aminobenzenesulphonic acid in Alcaligenes sp. (wikipedia.org)
  • The identification of 2,2'-diphenic acid in culture extracts indicates that phenanthrene was initially attacked through dioxigenation at C9 and C10 to give cis-9,10-dihydrodiol. (afpm.org.my)
  • This nanocomposite was prepared with slight modification of nanocrystalline cellulose (NCC) with cationic surfactant of cetyltriammonium bromide (CTAB) and further decorated with 3-mercaptopropionic acid (3-MPA) capped CdS QDs. (afpm.org.my)
  • The pcaH was significant in the β-ketoadipate pathway [9] where the pathway as it conserves biochemically, and is a major class of non-heme-iron containing dioxygenase [ 10 ]. (omicsonline.org)
  • The kinetic properties and thermal stability of the native and recombinant enzyme were identical. (muni.cz)
  • A mononuclear Fe(II)-dependent oxygenase, this enzyme catalyzes the conversion of homogentisate to 4-maleylacetoacetate, the third step in the pathway for the catabolism of TYROSINE. (umassmed.edu)
  • We examined kinetic parameters (K(m) and Vmax) for catechol 2,3-dioxygenase (C230), a key shared enzyme of the toluene-degradative pathway for these strains, and compared these parameters with those for the analogous enzymes from archetypal toluene-degrading pseudomonads which did not show enhanced, nitrate-dependent toluene degradation. (nih.gov)
  • C230 purified from strains W31, PKO1, and CFS215 had a significantly greater affinity for oxygen as well as a significantly greater rate of substrate turnover than found for the analogous enzymes from the TOL plasmid (pWW0) of Pseudomonas putida PaW1, from Pseudomonas cepacia G4, or from P. putida F1. (nih.gov)
  • Finally, the results of induction experiment indicated that thecatechol dioxygenases, both catechol 1,2-dioxygenase (C120) and catechol 2,3-dioxygenase (C230),were all inducible. (besjournal.com)
  • This enzyme catalyses the following chemical reaction catechol + O2 ⇌ {\displaystyle \rightleftharpoons } 2-hydroxymuconate semialdehyde This enzyme contains Fe(II). (wikipedia.org)
  • use AMINO ACIDS, BRANCHED-CHAIN 1979, & KETO ACIDS & VALERATES 1973-1979 MH - 3-Hydroxyanthranilate 3,4-Dioxygenase UI - D050561 MN - D8.811.682.690.416.328 MS - An enzyme that catalyzes the conversion of 3-hydroxyanthranilate to 2-amino-3-carboxymuconate semialdehyde. (nih.gov)
  • The genome sequence of R.pyridinivorans strain AK37 has identified several key enzymes involved in the six pathways of monocyclic aromatic compound biodegradation: protocatechuate 3,4-dioxygenase, benzoate 1,2-dioxygenase, 3-ketosteroid-9α-hydroxylase, 3-ketosteroid-σ-dehydrogenase, and so on. (kenyon.edu)
  • Deficiency in the enzyme causes ALKAPTONURIA, an autosomal recessive disorder, characterized by homogentisic aciduria, OCHRONOSIS and ARTHRITIS. (umassmed.edu)
  • use DICARBOXYLIC ACIDS 1970-1979 MH - 3-Phosphoshikimate 1-Carboxyvinyltransferase UI - D051229 MN - D8.811.913.225.735 MS - An enzyme of the shikimate pathway of AROMATIC AMINO ACID biosynthesis, it generates 5-enolpyruvylshikimate 3-phosphate and ORTHOPHOSPHATE from PHOSPHOENOLPYRUVATE and SHIKIMATE-3-PHOSPHATE. (nih.gov)
  • Conclusion The strains ZD 4-1 and ZD 4-3 metabolize phenol throughortho-pathways and meta-pathway, respectively. (besjournal.com)
  • Only has the pathway for BT biodegradation been proposed based on the identification of intermediate molecules (Fig. 3). (kenyon.edu)
  • Characterization of Phenol Biodegradation by Comamonas testosteroni ZD4-1 and Pseudomonas aeruginosa ZD4-3[J]. Biomedical and Environmental Sciences, 2003, 16(2): 163-172. (besjournal.com)
  • In addition, theresults of enzyme assays showed that the biodegradation efficiency of phenol by meta-pathways washigher than that by ortho-pathways. (besjournal.com)
  • This enzyme was formerly characterized as EC 1.13.1.2. (nih.gov)
  • This enzyme was formerly characterized as EC 1.13.1.5 and EC 1.99.2.5. (umassmed.edu)
  • in the degradative pathways of 2-aminobenzenesulphonic, benzenesulphonic and 4-toluenesulphonic acids in Alcaligenes sp. (wikipedia.org)
  • The strains ZD 4-1and ZD 4-3 metabolized phenol via ortho-pathways and meta-pathways, respectively. (besjournal.com)
  • Homogentisate 1,2-Dioxygenase" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (umassmed.edu)
  • Methods Bacterial strains ZD 4-1 and ZD4-3 were isolated by using phenol as the sole source of carbon and energy, and identified by 16SrDNA sequence analysis. (besjournal.com)
  • Results Bacterial strains ZD 4-1 and ZD 4-3 were identified asComamonas testosteroni and Pseudomonas aeruginosa by 16S rDNA sequence analysis, respectively.The growth of the two strains was observed on a variety of aromatic hydrocarbons. (besjournal.com)
  • Some of these enzymes seem to be widely distributed among aromatic-compound degrading species. (kenyon.edu)
  • HN - 2006(1981) MH - 2-Aminoadipate Transaminase UI - D051307 MN - D8.811.913.477.700.120 MS - A PYRIDOXAL PHOSPHATE containing enzyme that catalyzes the transfer of amino group of L-2-aminoadipate onto 2-OXOGLUTARATE to generate 2-oxoadipate and L-GLUTAMATE. (nih.gov)
  • HN - 2006(1981) BX - Cofilins MH - Actin-Related Protein 2 UI - D051377 MN - D5.750.78.730.246.500 MN - D12.776.220.525.246.500 MS - A PROFILIN binding domain protein that is part of the Arp2-3 complex. (nih.gov)
  • Analysis of the nucleotide and deduced amino acid sequences of C23O from strain PKO1 suggests that this extradiol dioxygenase belongs to a new cluster within the subfamily of C23Os that preferentially cleave monocyclic substrates. (nih.gov)
  • The 16S rDNA analysis revealed 99% nucleotide similarity to that of the type strain of R.rhodochrous (Fig. 2) ( 10 ). (kenyon.edu)
  • Objective To investigate the characteristic and biochemical mechanism about the phenolbiodegradation by bacterial strains ZD 4-1 and ZD 4-3. (besjournal.com)
  • This graph shows the total number of publications written about "Homogentisate 1,2-Dioxygenase" by people in this website by year, and whether "Homogentisate 1,2-Dioxygenase" was a major or minor topic of these publications. (umassmed.edu)
  • HN - 2006(1998) MH - Actin-Related Protein 2-3 Complex UI - D051376 MN - D5.750.78.730.246 MN - D12.776.220.525.246 MS - A complex of seven proteins including ARP2 PROTEIN and ARP3 PROTEIN that plays an essential role in maintenance and assembly of the CYTOSKELETON. (nih.gov)
  • Due to the recent research development on hydroquinone, this review underscores not only the mechanisms of hydroquinone biotransformation and the role of microorganisms and their enzymes in this process, but also its toxicity. (hindawi.com)
  • 9/3/2005) TOTAL DESCRIPTORS = 935 MH - 1-Acylglycerol-3-Phosphate O-Acyltransferase UI - D051103 MN - D8.811.913.50.173 MS - An enzyme that catalyzes the acyl group transfer of ACYL COA to 1-acyl-sn-glycerol 3-phosphate to generate 1,2-diacyl-sn-glycerol 3-phosphate. (nih.gov)