• The efficiency and regulatory properties of p ugp were measured using xylE and lacZ as reporter genes, which code for the corresponding enzymes catechol-2,3-dioxygenase (C23O) and β-galactosidase (βGal), respectively. (nau.edu)
  • Catechol 2,3-dioxygenase (C23O) gene was found andamplified with the designed primers from the total DNA of C-14-1. (researchgate.net)
  • The existence of catabolic and surfactant genes namely catechol dioxygenase ( C23O ), rhamnolipid enzyme ( rhlB ) and surfactin /lichenysin enzyme ( SrfA3 /LicA3 ) genes were detected in only four (4) out of the nine (9) marine aromatic degrading bacteria with 881 base pairs sizes. (journalarrb.com)
  • Moreover, a dioxygenase gene, nidA3B3, was detected in Mycobacterium vanbaalenii PYR-1 as an alternate degradation pathway which could catalyze both the initial dihydroxylation of pyrene [ 11 ] to be pyrene cis-4,5-dihydrodiol, and an alternate detoxification pyrene pathway to be pyrene cis-1,2-dihydrodiol [ 12 ]. (omicsonline.org)
  • New metabolic pathways of phenanthrene and a better understanding of the phenoloxidases and dioxygenase mechanism involved in degradation of phenanthrene were explored in this research. (afpm.org.my)
  • A number of bacteria that can degrade a variety of aromatic compounds have been identified and the pathways involved in the degradation have been extensively characterized [ 3 , 4 ]. (biomedcentral.com)
  • Further, the centralized degradation pathways mean synthesis of fewer degradative enzymes requiring less metabolic energy. (biomedcentral.com)
  • Overview: Tryptophan degradation in GapMind is based on MetaCyc degradation pathways I via anthranilate ( link ), II via pyruvate ( link ), or IX via 3-hydroxyanthranilate ( link ). (lbl.gov)
  • Comment: In MetaCyc pathway anthranilate degradation I ( link ), a dioxygenase cleaves off carbon dioxide and ammonia, leaving catechol. (lbl.gov)
  • In MetaCyc pathway anthranilate degradation IV ( link ), anthranilate hydroxylase/monooxygenase (hpaH) yields 3-hydroxyanthranilate. (lbl.gov)
  • Comment: 3-hydroxyanthranilate degradation is part of L-tryptophan degradation pathway XII ( link ). (lbl.gov)
  • Comment: In MetaCyc pathway catechol degradation to HPD I (meta-cleavage, link ), dioxygenase xylE converts catechol to (2Z,4E)-2-hydroxy-6-oxohexa-2,4-dienoate (also known as 2-hydroxymuconate 6-semialdehyde). (lbl.gov)
  • Catechol degradation to HPD II also involves xylE and HPD, link . (lbl.gov)
  • In MetaCyc pathway catechol degradation III (ortho-cleavage, link ), the 1,2-dioxygenase catA forms cis,cis-muconate, a cycloisomerase forms (+)-muconolactone, an isomerase converts this to (4,5-dihydro-5-oxofuran-2-yl)-acetate (also known as 3-oxoadipate enol lactone), and a hydrolase cleaves this to 3-oxoadipate. (lbl.gov)
  • Comment: MetaCyc pathway 3-oxoadipate degradation ( link ) involves activation by CoA (using succinyl-CoA) and a thiolase (succinyltransferase) reaction that splits it to acetyl-CoA and succinyl-CoA. (lbl.gov)
  • This series of steps is part of protocatechuate para-cleavage, link , or catechol degradation II, link . (lbl.gov)
  • This is part of a MetaCyc pathway for catechol degradation, link . (lbl.gov)
  • Comment: (2Z)-2-hydroxypenta-2,4-dienoate (HPD) is a common intermediate in the aerobic degradation of many aromatic compounds. (lbl.gov)
  • The enzyme protocatechuate 3,4-dioxygenase (3,4-PCD) catalyzes the ring cleavage of protocatechuate (PCA) in Acinetobacter baylyi. (uni-ulm.de)
  • A novel thermostable Mn(II)-dependent 2,3-dihydroxybiphenyl 1,2-dioxygenase (BphC_JF8) catalyzing the meta-cleavage of the hydroxylated biphenyl ring was purified from the thermophilic biphenyl and naphthalene degrader, Bacillus sp. (muni.cz)
  • An aromatic compound ring cleavage enzyme of catechol 1,2-dioxygenase was detected but catechol 2,3-dioxygenase was not detected in 2C6-43 T . A fatty acid profile with anteiso-C 15: 0 , iso-C 15: 0 and C 16: 0 as the major components supported the affiliation of strain 2C6-43 T to the genus Georgenia. (elsevierpure.com)
  • Dehydrogenation of phenanthrene-cis-9,10-dihydrodiol to produce the corresponding diol, followed by ortho-cleavage of the oxygenated ring, produced 2,2'-diphenic acid. (afpm.org.my)
  • David T. Gibson has included themes like Plasmid, DNA, Cleavage and Hydroquinone in his Enzyme study. (research.com)
  • In the convergent mode, structurally diverse aromatic compounds are converted to one of a few aromatic ring cleavage substrates such as catechol, gent sate, protocatechuate and their derivatives [ 5 ]. (biomedcentral.com)
  • In this divergent mode, a metal-dependent dioxygenase channels these dihydroxylated intermediates into one of the two possible pathways: the meta -cleavage pathway or the ortho -cleavage pathway [ 7 - 9 ] (Fig. 1 ). (biomedcentral.com)
  • Both enzymes are specialized for their substrate. (uni-ulm.de)
  • A blue white screening was established to identify mutants with acquired ability of 3,4-PCD to use catechol as substrate. (uni-ulm.de)
  • Analysis of 3,4-PCD activity toward catechol and PCA revealed further substitutions with changed substrate specificity. (uni-ulm.de)
  • The enzyme is resistant to denaturation by various chelators and inhibitors (EDTA, 1,10-phenanthroline, H2O2, 3-chlorocatechol) and did not exhibit substrate inhibition even at 3 mM 2,3-dihydroxybiphenyl. (muni.cz)
  • A 3-D homology model of BphC_JF8 provided a basis for understanding the substrate specificity, quaternary structure and stability of the enzyme. (muni.cz)
  • As the thiolate anion is not energetically favoured in the hydrophobic interior of the enzyme, it swings into the active site where it can be stabilized by the His202 imidazolium and the dipole of helix C. The Cys123 thiolate which now lies adjacent to the acyl carbon of the substrate, is thus generated only in the presence of substrate. (inrae.fr)
  • Oxygenases belong to the oxidoreductive group of enzymes (E.C. Class 1), which oxidize the substrates by transferring oxygen from molecular oxygen (O 2 ) and utilize FAD/NADH/NADPH as the co-substrate. (biomedcentral.com)
  • Peripheral enzymes, particularly oxygenases and dehydrogenases, were found to transform structurally diverse substrates into one of these central intermediates by bringing about the hydroxylation of the aromatic nucleus (Fig. 2A ), and hence it is thought that bacteria have developed these enzymes to extend their substrate range [ 5 ]. (biomedcentral.com)
  • Substrate specificity of catechol oxidase from Lycopus europaeus and characterization of the bioproducts of enzymic caffeic acid oxidation. (lookformedical.com)
  • The substrate specificity of catechol oxidase from Lycopus europaeus towards phenols is examined. (lookformedical.com)
  • Non-heme iron-containing enzymes that incorporate two atoms of OXYGEN into the substrate. (nih.gov)
  • When the xylE gene was maximally expressed, the induced enzyme constituted approx. (nau.edu)
  • 2006. Microbial dioxygenase gene population shifts during polycyclic aromatic hydrocarbon biodegradation. (nih.gov)
  • Each enzyme entry contains its common name and synonym, reaction in which enzyme is involved, family and subfamily, structure and gene link and literature citation. (biomedcentral.com)
  • Non-mammalian fat-1 gene prevents neoplasia when introduced to a mouse hepatocarcinogenesis model: Omega-3 fatty acids prevent liver neoplasia. (ouhsc.edu)
  • For example, enzymes from the CYP1, CYP2, and CYP3 gene families are responsible for most drug metabolism. (wakehealth.edu)
  • For all significantly differentially expressed microRNAs inside of the 2 groups, we now have produced gene sets from their expressed target genes. (pdpksignaling.com)
  • The concepts of his Pseudomonas putida study are interwoven with issues in Escherichia coli, Electron donor, Dioxygenase, Dehydrogenase and Naphthalene. (research.com)
  • p-Xylene-grown cells contained an inducible NAD+-dependent dehydrogenase which formed catechols from cis-p-toluate diol and the analogous acid diols formed from the other hydrocarbon substrates listed above. (rhea-db.org)
  • Glyceraldehyde 3-phosphate dehydrogenase [Interproscan]. (ntu.edu.sg)
  • Dioxygenase NbaC cleaves the aromatic ring, yielding 2-amino-3-carboxymuconate 6-semialdehyde, a decarboxylase forms (2Z,4E)-2-aminomuconate semialdehyde, a dehydrogenase forms (2Z,4E)-2-aminomuconate, a deaminase forms (3E)-2-oxo-3-hexenedioate (also known as 2-oxalocrotonate), and a decarboxylase forms (2Z)-2-hydroxypenta-2,4-dienoate (HPD). (lbl.gov)
  • Comment: Dehydrogenase praB forms 2-hydroxymuconate, tautomerase praC forms (3E)-2-oxohex-3-enedioate (2-oxalocrotonate), and decarboxylase praD yields 2-hydroxypenta-2,4-dienoate (HPD). (lbl.gov)
  • The metal ligands and active site residues of extradiol dioxygenases are conserved although several amino acid residues found exclusively in enzymes which preferentially cleave bicyclic substrates are missing in BphC_JF8. (muni.cz)
  • The enzymes responsible for growth on these substrates are encoded by a large plasmid with properties similar to those of TOL plasmids isolated from other strains of Pseudomonas. (rhea-db.org)
  • Substrates containing a -COOH group are inhibitors for catechol oxidase. (lookformedical.com)
  • 2) metabolism of endogenous and a wide variety of exogenous substrates, such as toxins and drugs (BIOTRANSFORMATION). (wakehealth.edu)
  • Cloning and characterization of a novel thermostable Mn(II)-dependent 2,3-dihydroxybiphenyl 1,2-dioxygenase from a PCB and naphthalene degrading Bacillus sp. (muni.cz)
  • 2003. Characterization of a novel Acinetobacter species containing a toluene dioxygenase. (nih.gov)
  • 1. Fischer, D., Ebenau-Jehle, C. and Grisebach, H. Phytoalexin synthesis in soybean: purification and characterization of NADPH:2'-hydroxydaidzein oxidoreductase from elicitor-challenged soybean cell cultures. (qmul.ac.uk)
  • Dienelactone hydrolase (DLH), an enzyme from the beta-ketoadipate pathway, catalyzes the hydrolysis of dienelactone to maleylacetate. (inrae.fr)
  • The root-mean-square deviation from ideal bond distances for the model is 0.014 A and the error in atomic co-ordinates is estimated to be 0.15 A. DLH is a monomeric enzyme containing 236 amino acid residues and is a member of the beta-ketoadipate pathway found in bacteria and fungi. (inrae.fr)
  • Enzymes induced in 4-fluorobenzoate-utilizing bacteria have been classified into three groups on the basis of their specificity towards cis- and trans-dienelactone. (inrae.fr)
  • PAHs could be degraded by bacteria under aerobic conditions through the initial oxidation of the aromatic ring, which is catalyzed by the dioxygenase enzyme. (omicsonline.org)
  • then, oxygenase sibC forms 3-hydroxy-L-kynurenine, which is hydrolyzed to L-alanine and 3-hydroxyanthranilate. (lbl.gov)
  • nidA and nidB genes are encoding genes for large and small subunits of napthalene-inducible dioxygenase [ 7 , 8 ]. (omicsonline.org)
  • B, solute carrier relatives six member 2, solute carrier relatives 18 member one, and transcription factors and homeobox genes involved in neural crest derived cell de velopment, paired like homeobox 2a and 2b, GATA binding protein two and 3, heart and neural crest derivatives expressed two. (pdpksignaling.com)
  • The native and recombinant BphC enzyme was purified to homogeneity. (muni.cz)
  • Prophenoloxidase-activating enzyme (PPAE) was purified to homogeneity as judged by SDS-polyacrylamide gel electrophoresis from larval cuticles of the silkworm, Bombyx mori. (lookformedical.com)
  • The pcaH was significant in the β-ketoadipate pathway [9] where the pathway as it conserves biochemically, and is a major class of non-heme-iron containing dioxygenase [ 10 ]. (omicsonline.org)
  • An analysis of the genome and its transcriptome revealed enzymes with biotechnological potential, such as two extracellular tannases (Atan1p and Atan2p) of the tannic-acid catabolic route, and a new pathway for the assimilation of n-butanol via butyric aldehyde and butyric acid. (biomedcentral.com)
  • For example, pathway IV yields indole-3-lactate, which could potentially be oxidized to indole-3-acetate, which has a known catabolic pathway, but no prokaryotes are known to consume tryptophan this way. (lbl.gov)
  • Pathway VIII yields tryptophol (also known as indole-3-ethanol), which could potentially be oxidized to indole-3-acetate and consumed. (lbl.gov)
  • Comment: In pathway I, dioxygenase kynA opens the non-aromatic ring, to N-formyl-L-kynureine, a hydrolase yields L-kynurenine (and formate), and a hydrolase yields anthranilate and L-alanine. (lbl.gov)
  • It exhibited a half life of 30 min at 80 C and 81 min at 75 C, making it the most thermostable extradiol dioxygenase studied. (muni.cz)
  • An integrated approach of bioassay and molecular docking to study the dihydroxylation mechanism of pyrene by naphthalene dioxygenase in Rhodococcus sp. (brenda-enzymes.org)
  • Identification of metabolites from phenanthrene oxidation by phenoloxidases and dioxygenases of Polyporus sp. (afpm.org.my)
  • An enzyme that catalyzes the oxidation of catechol to muconic acid with the use of Fe3+ as a cofactor. (nih.gov)
  • The enzyme catalyzes the oxidation of o-diphenols to o-quinones without hydroxylating monophenols, the additional activity of tyrosinase. (lookformedical.com)
  • An enzyme that catalyzes the oxidation of BENZOATE to 4-hydroxybenzoate. (uchicago.edu)
  • TIM barrel domain, Glyoxalase/fosfomycin resistance/dioxygenase domain, Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily [InterProScan]. (ntu.edu.sg)
  • Comment: There are two forms of anthranilate dioxygenase, 3-subunit antABC or 4-subunit andAabcd. (lbl.gov)
  • 2023, 43(3): 297-309. (tea-science.com)
  • Analysis of Codon Usage Bias and Phylogenesis in the Chloroplast Genome of Ancient Tea Tree Camellia taliensis in Forest-tea Garden[J]. Journal of Tea Science, 2023, 43(3): 297-309. (tea-science.com)
  • 2023, 43(3): 310-324. (tea-science.com)
  • Thea bohea L.) Based on GBS-SNP[J]. Journal of Tea Science, 2023, 43(3): 310-324. (tea-science.com)
  • Catechol 1,2-dioxygenase is structurally similar to 3,4-PCD. (uni-ulm.de)
  • Oxygenation and spontaneous deamination of 2-aminobenzenesulphonic acid in Alcaligenes sp. (wikipedia.org)
  • in the degradative pathways of 2-aminobenzenesulphonic, benzenesulphonic and 4-toluenesulphonic acids in Alcaligenes sp. (wikipedia.org)
  • This nanocomposite was prepared with slight modification of nanocrystalline cellulose (NCC) with cationic surfactant of cetyltriammonium bromide (CTAB) and further decorated with 3-mercaptopropionic acid (3-MPA) capped CdS QDs. (afpm.org.my)
  • Additional pathways are not included: the fate of 2-amino-5-oxocyclohex-1-enecarboxyl-CoA is not known ( link ), and anthraniloyl-CoA reductase (the only anaerobic route known, link ) has not been linked to sequence. (lbl.gov)
  • The ugp promoter (p ugp ) responsible for expression of the binding-protein-dependent sn-glycerol-3-phosphate transport system in Escherichia coli was cloned into a small multicopy plasmid pTER5, a derivative of pBR322, between the transcription terminators rpoCt and t L1 . (nau.edu)
  • protein_coding" "AAC74185","ptsG","Escherichia coli","fused glucose-specific PTS enzymes: IIB component/IIC component [Ensembl]. (ntu.edu.sg)
  • protein_coding" "AAC74888","manY","Escherichia coli","mannose-specific enzyme IIC component of PTS [Ensembl]. (ntu.edu.sg)
  • This enzyme catalyses the following chemical reaction catechol + O2 ⇌ {\displaystyle \rightleftharpoons } 2-hydroxymuconate semialdehyde This enzyme contains Fe(II). (wikipedia.org)
  • However, studies could show that a R133H substitution switches the specificity of 3,4-PCD towards catechol. (uni-ulm.de)
  • Pathways X and XIII yield indole-3-propionate, which may spontaneously oxidize to kynurate, but kynurate catabolism is not reported. (lbl.gov)
  • ICP-MS analysis confirmed the presence of 4.0 to 4.8 manganese atoms per enzyme molecule. (muni.cz)
  • bioremediation of 3,5-dinitrobenzoic acid and aniline by a corynebacterium sp. (academicjournals.org)
  • Crystal structure of naphthalene dioxygenase: side-on binding of dioxygen to iron. (research.com)
  • The nanocomposite material was then employed as scaffold for immobilization of tyrosinase enzyme (Tyr). (afpm.org.my)
  • In the reverse reaction, the 2'-hydroxyisoflavone (2'-hydroxydaidzein) is reduced to an isoflavanone. (qmul.ac.uk)
  • The primary structure of BphC_JF8 exhibits less than 25% sequence identity to other 2,3-dihydroxybiphenyl 1,2-dioxygenases. (muni.cz)
  • This competitive enzyme-linked immunosorbent assay-like method, used competitive binding of free hyaluronan in the sample and biotin-labeled standard hyaluronan to hyaluronan binding protein in solid phase. (go.jp)