PseudomonasCatalytic mechanism of catecholCleavageGeneEnzymesDegradationPathwayExtradiol dioxygenaseMuconic acidCleavesDehydrogenasePurification and PropertiesSuperfamilyAnthranilateStrainBioremediationBenzoateMechanismAcinetobacterBacteriaAcidPhenanthreneSubstrateGenesCarbonAminoProteinSequenceRegulationTemperatureDataSmallTypeFoundMeta
Pseudomonas4
- Thus far, 1,2-CTD has been observed to exist in the following species of soil bacteria and fungi: Pseudomonas sp. (wikipedia.org)
- the 1,2-CTD enzyme produced by Pseudomonas arvilla is the exception to this rule, containing two highly homologous subunits that can form either a homo- or hetero- dimer. (wikipedia.org)
- The aim of the study was to purify, characterize and predict template-based three-dimensional structure of catechol 1,2-dioxygenase (C12O) from indigenous Pseudomonas chlororaphis strain UFB2 ( Pc UFB2). (preprints.org)
- ES-MS spectra of tryptic digested SDS-PAGE band and bioinformatics studies revealed that C12O share 81% homology to homogentisate 1,2-dioxygenase reported in other Pseudomonas chlororaphis strains. (preprints.org)
Catalytic mechanism of catechol1
- The catalytic mechanism of catechol 1,2-dioxygenase was elucidated using a combination of O18 labeling experiments and crystallography. (wikipedia.org)
Cleavage10
- A novel thermostable Mn(II)-dependent 2,3-dihydroxybiphenyl 1,2-dioxygenase (BphC_JF8) catalyzing the meta-cleavage of the hydroxylated biphenyl ring was purified from the thermophilic biphenyl and naphthalene degrader, Bacillus sp. (muni.cz)
- 2021. Separate upper pathway ring cleavage dioxygenases are required for growth of Sphingomonas wittichii strain RW1 on dibenzofuran and dibenzo-p-dioxin. (nih.gov)
- The enzyme protocatechuate 3,4-dioxygenase (3,4-PCD) catalyzes the ring cleavage of protocatechuate (PCA) in Acinetobacter baylyi. (uni-ulm.de)
- Comment: In MetaCyc pathway catechol degradation to HPD I (meta-cleavage, link ), dioxygenase xylE converts catechol to (2Z,4E)-2-hydroxy-6-oxohexa-2,4-dienoate (also known as 2-hydroxymuconate 6-semialdehyde). (lbl.gov)
- In MetaCyc pathway catechol degradation III (ortho-cleavage, link ), the 1,2-dioxygenase catA forms cis,cis-muconate, a cycloisomerase forms (+)-muconolactone, an isomerase converts this to (4,5-dihydro-5-oxofuran-2-yl)-acetate (also known as 3-oxoadipate enol lactone), and a hydrolase cleaves this to 3-oxoadipate. (lbl.gov)
- This series of steps is part of protocatechuate para-cleavage, link , or catechol degradation II, link . (lbl.gov)
- It is important to emphasize that the isolation of soil microorganisms on a mineral medium with benzoate made it possible to quickly assess the presence of one of two biodegradative pathways - ortho - or meta -cleavage of catechol. (sciforum.net)
- The data obtained did not allow us to detect strains decomposing benzoate via the pathway of meta -cleavage, since there was no yellow coloration of the medium, characteristic of 2-hydroxymuconic semialdehyde formed as a result of meta -cleavage of catechol, a product of benzoate biodegradation. (sciforum.net)
- An aromatic compound ring cleavage enzyme of catechol 1,2-dioxygenase was detected but catechol 2,3-dioxygenase was not detected in 2C6-43 T . A fatty acid profile with anteiso-C 15: 0 , iso-C 15: 0 and C 16: 0 as the major components supported the affiliation of strain 2C6-43 T to the genus Georgenia. (elsevierpure.com)
- Dehydrogenation of phenanthrene-cis-9,10-dihydrodiol to produce the corresponding diol, followed by ortho-cleavage of the oxygenated ring, produced 2,2'-diphenic acid. (afpm.org.my)
Gene4
- Catechol 2,3-dioxygenase (C23O) gene was found andamplified with the designed primers from the total DNA of C-14-1. (researchgate.net)
- 2006. Microbial dioxygenase gene population shifts during polycyclic aromatic hydrocarbon biodegradation. (nih.gov)
- Moreover, a dioxygenase gene, nidA3B3, was detected in Mycobacterium vanbaalenii PYR-1 as an alternate degradation pathway which could catalyze both the initial dihydroxylation of pyrene [ 11 ] to be pyrene cis-4,5-dihydrodiol, and an alternate detoxification pyrene pathway to be pyrene cis-1,2-dihydrodiol [ 12 ]. (omicsonline.org)
- For all significantly differentially expressed microRNAs inside of the 2 groups, we now have produced gene sets from their expressed target genes. (pdpksignaling.com)
Enzymes3
- The two enzymes were identified to be a part of two separate catechol dioxygenase families: 1,2-CTD was classified as an intradiol dioxygenase while 2,3-CTD was classified as an extradiol dioxygenase. (wikipedia.org)
- The metal ligands and active site residues of extradiol dioxygenases are conserved although several amino acid residues found exclusively in enzymes which preferentially cleave bicyclic substrates are missing in BphC_JF8. (muni.cz)
- The efficiency and regulatory properties of p ugp were measured using xylE and lacZ as reporter genes, which code for the corresponding enzymes catechol-2,3-dioxygenase (C23O) and β-galactosidase (βGal), respectively. (nau.edu)
Degradation6
- These bacteria subsequently employ 1,2-CTD in the last step of the degradation of aromatic compounds to aliphatic products. (wikipedia.org)
- Comment: In MetaCyc pathway anthranilate degradation I ( link ), a dioxygenase cleaves off carbon dioxide and ammonia, leaving catechol. (lbl.gov)
- Catechol degradation to HPD II also involves xylE and HPD, link . (lbl.gov)
- This is part of a MetaCyc pathway for catechol degradation, link . (lbl.gov)
- Comment: (2Z)-2-hydroxypenta-2,4-dienoate (HPD) is a common intermediate in the aerobic degradation of many aromatic compounds. (lbl.gov)
- New metabolic pathways of phenanthrene and a better understanding of the phenoloxidases and dioxygenase mechanism involved in degradation of phenanthrene were explored in this research. (afpm.org.my)
Pathway4
- Catechol dioxygenases in microorganisms cleave catechol into cis - cis -muconic acid or 2-hydroxymuconic semialdehyde via the ortho- or meta- pathway, respectively. (preprints.org)
- Characterization and optimization of C12O activity can assist in understanding the 2,4-DCP metabolic pathway in Pc UFB2 and its possible application in bioremediation strategies. (preprints.org)
- The pcaH was significant in the β-ketoadipate pathway [9] where the pathway as it conserves biochemically, and is a major class of non-heme-iron containing dioxygenase [ 10 ]. (omicsonline.org)
- Comment: In pathway I, dioxygenase kynA opens the non-aromatic ring, to N-formyl-L-kynureine, a hydrolase yields L-kynurenine (and formate), and a hydrolase yields anthranilate and L-alanine. (lbl.gov)
Extradiol dioxygenase1
- It exhibited a half life of 30 min at 80 C and 81 min at 75 C, making it the most thermostable extradiol dioxygenase studied. (muni.cz)
Muconic acid3
- 1,2-CTD uses Fe3+ as a cofactor to cleave the carbon-carbon bond between the phenolic hydroxyl groups of catechol, thus yielding muconic acid as its product. (wikipedia.org)
- They synthesized catechol 1, 2 - dioxygense enzyme which is unique its properties to transform catechol to cis, cis - muconic acid. (researchbib.com)
- An enzyme that catalyzes the oxidation of catechol to muconic acid with the use of Fe3+ as a cofactor. (nih.gov)
Cleaves1
- Dioxygenase NbaC cleaves the aromatic ring, yielding 2-amino-3-carboxymuconate 6-semialdehyde, a decarboxylase forms (2Z,4E)-2-aminomuconate semialdehyde, a dehydrogenase forms (2Z,4E)-2-aminomuconate, a deaminase forms (3E)-2-oxo-3-hexenedioate (also known as 2-oxalocrotonate), and a decarboxylase forms (2Z)-2-hydroxypenta-2,4-dienoate (HPD). (lbl.gov)
Dehydrogenase2
- Comment: Dehydrogenase praB forms 2-hydroxymuconate, tautomerase praC forms (3E)-2-oxohex-3-enedioate (2-oxalocrotonate), and decarboxylase praD yields 2-hydroxypenta-2,4-dienoate (HPD). (lbl.gov)
- p-Xylene-grown cells contained an inducible NAD+-dependent dehydrogenase which formed catechols from cis-p-toluate diol and the analogous acid diols formed from the other hydrocarbon substrates listed above. (rhea-db.org)
Purification and Properties1
- Catechol 1,2-dioxygenase from Acinetobacter calcoaceticus: purification and properties. (shengsci.com)
Superfamily1
- TIM barrel domain, Glyoxalase/fosfomycin resistance/dioxygenase domain, Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily [InterProScan]. (ntu.edu.sg)
Anthranilate1
- Comment: There are two forms of anthranilate dioxygenase, 3-subunit antABC or 4-subunit andAabcd. (lbl.gov)
Strain2
- Procedures for the purification of catechol 1,2-dioxygenase from extracts of Acinetobacter calcoaceticus strain ADP-96 are described. (shengsci.com)
- Treatment of P. putida BG1 with nitrosoguanidine led to the isolation of a mutant strain which, when grown with fructose, oxidized both p-xylene and p-toluate to (-)-cis-1,2-dihydroxy-4-methylcyclohexa-3,5-diene-1-carboxylic acid (cis-p-toluate diol). (rhea-db.org)
Bioremediation1
- Therefore, engineering of Catechol 2,3-dioxygenase may be employed for application on bioremediation of polluted sites. (openmicrobiologyjournal.com)
Benzoate1
- Thus, it was shown that the metabolism of benzoate in the isolated strains occurs with the induction of catechol 1,2-dioxygenase. (sciforum.net)
Mechanism1
- Exploring the catalytic mechanism of the extradiol catechol dioxygenases. (warwick.ac.uk)
Acinetobacter1
- 2003. Characterization of a novel Acinetobacter species containing a toluene dioxygenase. (nih.gov)
Bacteria1
- PAHs could be degraded by bacteria under aerobic conditions through the initial oxidation of the aromatic ring, which is catalyzed by the dioxygenase enzyme. (omicsonline.org)
Acid4
- Pre-Steady-State Kinetic Analysis of 2-Hydroxy-6-keto-nona-2,4-diene-1,9-dioic Acid 5,6-Hydrolase: Kinetic Evidence for Enol/Keto Tautomerization. (warwick.ac.uk)
- Purification, Characterization, and Stereochemical Analysis of a C-C Hydrolase: 2-Hydroxy-6-keto-nona-2,4-diene-1,9-Dioic Acid 5,6-Hydrolase. (warwick.ac.uk)
- Chemical and biochemical properties of 2-hydroxypentadienoic acid, a homolog of enolpyruvic acid. (warwick.ac.uk)
- The identification of 2,2'-diphenic acid in culture extracts indicates that phenanthrene was initially attacked through dioxigenation at C9 and C10 to give cis-9,10-dihydrodiol. (afpm.org.my)
Phenanthrene1
- Identification of metabolites from phenanthrene oxidation by phenoloxidases and dioxygenases of Polyporus sp. (afpm.org.my)
Substrate3
- The enzyme is resistant to denaturation by various chelators and inhibitors (EDTA, 1,10-phenanthroline, H2O2, 3-chlorocatechol) and did not exhibit substrate inhibition even at 3 mM 2,3-dihydroxybiphenyl. (muni.cz)
- A blue white screening was established to identify mutants with acquired ability of 3,4-PCD to use catechol as substrate. (uni-ulm.de)
- Analysis of 3,4-PCD activity toward catechol and PCA revealed further substitutions with changed substrate specificity. (uni-ulm.de)
Genes2
- nidA and nidB genes are encoding genes for large and small subunits of napthalene-inducible dioxygenase [ 7 , 8 ]. (omicsonline.org)
- B, solute carrier relatives six member 2, solute carrier relatives 18 member one, and transcription factors and homeobox genes involved in neural crest derived cell de velopment, paired like homeobox 2a and 2b, GATA binding protein two and 3, heart and neural crest derivatives expressed two. (pdpksignaling.com)
Carbon2
- In contrast, 2,3-CTD utilizes Fe2+ as a cofactor to cleave the carbon-carbon bond adjacent to the phenolic hydroxyl groups of catechol, thus yielding 2-hydroxymuconaldehye as its product. (wikipedia.org)
- The second catechol hydroxyl group on carbon 3 (C3) is coordinated to Fe3+ after its deprotonation by the Tyr200 ligand. (wikipedia.org)
Amino1
- Additional pathways are not included: the fate of 2-amino-5-oxocyclohex-1-enecarboxyl-CoA is not known ( link ), and anthraniloyl-CoA reductase (the only anaerobic route known, link ) has not been linked to sequence. (lbl.gov)
Protein1
- The enzyme contained 2 g-atoms of iron per mol of protein. (shengsci.com)
Sequence1
- The primary structure of BphC_JF8 exhibits less than 25% sequence identity to other 2,3-dihydroxybiphenyl 1,2-dioxygenases. (muni.cz)
Regulation1
- Two alpha-ketoglutarate dependent non-heme iron dioxygenases are responsible for the regulation of HIF-1 through hydroxylation of residues on the HIF-1a subunit. (umass.edu)
Temperature2
- Specific activities of Catechol-2,3-dioxygenase and effects of temperature and pH and their stabilities on the enzyme relative activities were observed. (openmicrobiologyjournal.com)
- A decrease in Km accompanied an increase in temperature and the Km value of 0.095 uM for 2,3-dihydroxybiphenyl (at 60 C) is among the lowest reported. (muni.cz)
Data2
- Progress towards SDG target 6.1, the proportion of humans with "universal and equitable access to safe and affordable drinking water" is tracked primarily using data on drinking water infrastructure access reported by national statistics offices to the United Nation's Children's Emergency Fund (UNICEF) and the World Health Organization's (WHO) Joint Monitoring Programme (JMP) 2 . (nature.com)
- Current estimates based on JMP data indicate that two billion people worldwide lack access to safely managed drinking water service 2 , such that we are not on track to meet target 6.1 by 2030 3 . (nature.com)
Small1
- It was hypothesized that there would be a difference in inhibition of FIH-1 from the other HIF-1 regulating enzyme, the prolyl hydroxylase domain-2 (PHD2), when testing a series of ten small molecule inhibitors. (umass.edu)
Type1
- Several classes of cannabinoid receptor type 2 radioligands have been evaluated for imaging of neuroinflammation, with successful clinical translation yet to take place. (bvsalud.org)
Found1
Meta1
- The catechols were converted to meta ring fission products by an inducible catechol-2,3-dioxygenase which was partially purified from p-xylene-grown cells of P. putida BG1. (rhea-db.org)