• NIP3 is a member of the BCL2/adenovirus E1B 19 kd-interacting protein (BNIP) family. (thermofisher.com)
  • It interacts with the E1B 19 kDa protein which is responsible for the protection of virally-induced cell death, as well as E1B 19 kDa-like sequences of BCL2, also an apoptotic protector. (thermofisher.com)
  • The dimeric mitochondrial protein is known to induce apoptosis, even in the presence of BCL2. (thermofisher.com)
  • BCL2 and adenovirus E1B 19-kDa interacting protein 3 (BNIP3), a well-known atrogene, was identified as a potential mediator of muscle fiber atrophy in LOPD muscle biopsies. (nih.gov)
  • The encoded protein interacts with anti-apoptotic proteins, including the E1B 19 kDa protein and Bcl2. (modellab.cn)
  • This protein appears to contain 4 transmembrane domains, is a substrate for cAMP-dependent protein kinase and protein kinase C, and binds with calmodulin. (wikipedia.org)
  • The current data suggested that BNIP3 expression is regulated by inhibition of the AKT-mammalian target of rapamycin pathway, leading to phosphorylation of Unc-51 like autophagy activating kinase 1 (ULK1) at Ser317 by AMP-activated protein kinase. (nih.gov)
  • Titin is a giant elastic protein with kinase activity that extends half the length of a sarcomere. (thermofisher.cn)
  • TCAP is a protein found in striated and cardiac muscle that binds to the titin Z1-Z2 domains and is a substrate of titin kinase, interactions thought to be critical to sarcomere assembly. (thermofisher.cn)
  • These paclitaxel resistant ovarian cells demonstrate: (1) Increased IC 50 for paclitaxel and docetaxel (10 to 75-fold) and cross-resistance to anthracyclines (2) Reduced cell apoptosis in the presence of paclitaxel (3) Gene depletion involving mitotic regulators BUB1 mitotic checkpoint serine/threonine kinase, cyclin BI (CCNB1), centromere protein E (CENPE), and centromere protein F (CENPF), and (4) Functional data validating gene depletion among mitotic regulators. (biomedcentral.com)
  • Market growth has been supported by advances in recombinant protein production technologies, including the engineering of expression hosts, the optimization of upstream cultivation (e.g., bioreactor design, nutritional, and physical parameters), and the development of more efficient protein extraction and purification methods. (frontiersin.org)
  • Considering the potential limitations of plasma derived Hp, recombinant protein production may offer a relevant strategy for production. (biomedcentral.com)
  • Additionally, recombinant protein-production strategies may also generate therapeutics with enhanced functionality, bioavailability and pharmacokinetics. (biomedcentral.com)
  • The key regulator of this process, hypoxia inducible factor 1 (HIF-1), can initiate apoptosis by inducing high concentrations of proapoptotic proteins, such as BNIP3, and can cause stabilisation of p53. (bmj.com)
  • Retracted: Bcl-2 19-kDa Interacting Protein 3 (BNIP3)-Mediated Mitophagy Attenuates Intermittent Hypoxia-Induced Human Renal Tubular Epithelial Cell Injury. (nih.gov)
  • BNIP3 (Bcl-2/ adenovirus E1B 19-kDa-interacting protein 3) is a receptor accounting for priming damaged mitochondria for autophagic removal. (chinaphar.com)
  • The mRNA expression of HIF target genes, namely glucose transporter-1, carbonic anhydrase-9, vascular endothelial growth factor, B-cell lymphoma 2/adenovirus E1B 19-kDa protein-interacting protein 3, prolyl hydroxylase domain-2, and prolyl hydroxylase domain-3, was not significantly altered in NVVs and VVs exposed to hypoxia and treated with doxycycline or MPFF compared with those untreated. (ox.ac.uk)
  • Lens fiber membrane intrinsic protein is a protein that in humans is encoded by the LIM2 gene. (wikipedia.org)
  • 1278\1278\BAD35103.1\Human adenovirus type 4\Human adenovirus type 4 gene for fiber protein, complete cds,isolate:1998-4439. (or.jp)
  • 1278\1278\BAD35104.1\Human adenovirus type 4\Human adenovirus type 4 gene for fiber protein, complete cds,isolate:TC-18040. (or.jp)
  • 1278\1278\BAD35105.1\Human adenovirus type 4\Human adenovirus type 4 gene for fiber protein, complete cds,isolate:TC-18465. (or.jp)
  • The protein encoded by this gene belongs to the complex I 19 kDa subunit family. (nih.gov)
  • Description of the protein which includes the UniProt Function and the NCBI Gene Summary. (nih.gov)
  • Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in myxoid liposarcomas or Ewing sarcoma. (novusbio.com)
  • Further analysis indicated that these two proteins possessed almost identical gene sequences, apart from two insertion/deletion events in predicted external loops present within the putative barrel-like structure of the proteins. (eur.nl)
  • The Nano Luciferase (NanoLuc) which was introduced in 2013 by Promega is a new member of the luciferase reporter gene/protein familiy and shows some advantages compared to the other family members. (igem.org)
  • Mutations in TCAP gene are associated with limb-girdle muscular dystrophy type 2G.Sarcomere assembly is regulated by the muscle protein titin. (thermofisher.cn)
  • Among these hub genes, Guanylate binding protein 2b (GBP2b) was selected as a key gene for further investigation. (researchsquare.com)
  • Furthermore, gene co-expression analysis and further experiments showed that GBP2b regulates M1 polarization through activation of Toll-like receptor 4 and nuclear factor-κBp65 protein. (researchsquare.com)
  • This gene is encodes a mitochondrial protein that contains a BH3 domain and acts as a pro-apoptotic factor. (modellab.cn)
  • These interactions provide a temporal and special framework for the work of the cell, controlling gene expression, protein production, growth, cell division and cell death. (promegaconnections.com)
  • Therapeutic proteins (e.g., antibodies, vaccines, enzymes, cytokines, and growth factors) account for almost half of this market, followed by industrial proteins (e.g., technical enzymes) and research reagents (e.g., antibodies for protein detection and purification) ( Markets and Markets, 2017 ). (frontiersin.org)
  • The rPvRAMA protein induced antibody responses, with the end-point titer ranging from 1:10,000 to 1:5,120,000. (biomedcentral.com)
  • The 152 amino acid recombinant protein has a predicted molecular mass of 17,394 Da. (biolegend.com)
  • Prolamin proteins define nutritional value of maize grain not only via amino acid quality but also via starch availability. (intechopen.com)
  • Surprisingly, sequenced strains from four animal S. aureus strains had surface and immune evasion proteins remarkably similar to those found in human strains, yet putative targets of these proteins vary substantially between different hosts. (biomedcentral.com)
  • However, the most variant protein domains were the putative functional regions and there is biological evidence that variants can be functional, arguing they do play a role. (biomedcentral.com)
  • The function of a protein is determined by the number and sequence of amino acids, which controls the three-dimensional structure of the resulting folded polypeptide. (frontiersin.org)
  • Whole genome sequences of 58 strains of S. aureus in the public domain and data from multi-strain microarrays were compared to assess variation in the sequence of proteins known or putatively interacting with host. (biomedcentral.com)
  • Preclinical studies have evaluated haptoglobin (Hp) polymers from pooled human plasma as a therapeutic protein to attenuate toxic effects of cell-free hemoglobin (Hb). (biomedcentral.com)
  • The second most abundant intrinsic membrane protein of the lens fiber cell is MP19, so named for major lens protein having a molecular weight of 19.5 kD. (wikipedia.org)
  • RePub, Erasmus University Repository: Identification and characterization of a novel outer membrane protein (OMPJ) of Moraxella catarrhalis that exists in two major forms. (eur.nl)
  • In this publication, we characterize a novel outer membrane protein of Moraxella catarrhalis which exists in two variant forms associated with particular genetic lineages, and both forms are suggested to contribute to bacterial clearance from the lungs. (eur.nl)
  • Plasmodium vivax rhoptry-associated membrane antigen (RAMA) is a glycophosphatidylinositol-anchored membrane protein currently under consideration as a malaria vaccine candidate. (biomedcentral.com)
  • This approach demonstrates that efficient expression of mature and fully functional protein products requires co-expression of active C1r-LP. The functional characterization of our proteins, which included monomer/polymer distribution, binding affinities as well as NO-sparing and antioxidant functions, demonstrated that C1r-LP-processed recombinant Hp demonstrates equal protective functions as plasma derived Hp in vitro as well as in animal studies. (biomedcentral.com)
  • Interactions between the macromolecules within cells, including proteins, are ever changing-with complexes forming, breaking up, and reforming in new ways. (promegaconnections.com)
  • BRET (bioluminescence resonance energy transfer) has been applied to studies of protein interactions in cells for over 15 years. (promegaconnections.com)
  • Additionally the NanoBRET™ measurement remained relatively constant for nearly an hour, indicating that the NanoBRET™ system would be useful for characterizing protein:protein interactions (PPI) over time. (promegaconnections.com)
  • Many surface protein genes were missing or truncated, unlike immune evasion genes, and several distinct variants were identified. (biomedcentral.com)
  • Mutations in the MC2 receptor accessory protein (MRAP) are responsible for another estimated 15-20% of cases of FGD. (medscape.com)
  • Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes. (novusbio.com)
  • After the SA-chromatography the protein was concentrated using centrifugal concentration units (MWCO: 10 kDa). (igem.org)
  • MSP1 19 , 19-kDa merozoite surface protein. (cdc.gov)
  • We have recently conducted a first-in-human clinical trial to evaluate the safety and immunogenicity of the recombinant, full-length merozoite surface protein 1 (MSP1 FL ) formulated with GLA-SE as adjuvant. (nature.com)
  • Within this matrix, starch granules are surrounded by protein bodies that limit starch availability. (intechopen.com)
  • EDN is released from eosinophil granules together with eosinophil cationic proteins and eosinophil peroxidise (EPO). (ersjournals.com)
  • Cellular experimental studies confirm that GBP2b was a novel regulatory molecule that promotes M1 macrophage polarization during M. bovis infection and was also an antimicrobial protein that promotes intracellular bacterial clearance. (researchsquare.com)
  • HaloTag® Technology consists of a protein tag that covalently binds a chloroalkane tag that can be chemically coupled to fluorophores or other molecules. (promegaconnections.com)
  • Classes for this protein according to by Drug Target Ontology (DTO). (nih.gov)
  • Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. (novusbio.com)
  • Plasmodium falciparum rhoptry-associated protein 1 (PfRAP1) and P. falciparum high-molecular-weight rhoptry protein 3 (PfRhopH3) have been proved to be escorted to the rhoptry protein via an interaction with the PfRAMA [ 7 ]. (biomedcentral.com)
  • In this study, the binding affinity and mechanism of human odor-binding protein OBP2a to 14 typical odorants in water were first assessed using fluorescent competitive binding assays and molecular docking techniques. (springeropen.com)
  • The most commonly used BRET method (BRET1) relies on an engineered luciferase from sea pansy ( Renilla luciferase), Rluc8, which can provide higher light intensity than traditional luciferase donors, matched with Yellow Fluorescent Protein (YFP). (promegaconnections.com)
  • NanoLuc® Luciferase (Nluc) is a very small (19 KDa) protein that is physically very stable and produces an extremely bright signal. (promegaconnections.com)
  • This chapter will give insight into genotype and growing conditions influencing the quantity and quality of storage proteins, primarily maize storage proteins the leading cereal by world production. (intechopen.com)
  • Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. (biolegend.com)
  • Afterwards the lysate was applied to a Streptavidin-Affinity (SA) column and was subsequently washed using SA-Buffer until a baseline was reached and the protein was then eluted using 5 mM of biotin (Attention: These are special columns which are not availible commercially. (igem.org)
  • The protein functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, such as C/EBP and LAP (liver activator protein), and preventing their DNA binding activity. (novusbio.com)
  • RESULTS: Treatment of NVV organ cultures exposed to hypoxia with doxycycline or MPFF did not significantly alter the expression of HIF-1α and HIF-2α messenger (m)RNA and protein compared with untreated. (ox.ac.uk)
  • Doxycycline also did not significantly affect the expression of HIF-1α and HIF-2α mRNA and protein in VVs exposed to hypoxia compared with untreated VVs. (ox.ac.uk)
  • Apoptosis regulatory proteins are delicately balanced. (bmj.com)
  • The protein is implicated in adipogenesis and erythropoiesis, is activated by endoplasmic reticulum stress, and promotes apoptosis. (novusbio.com)
  • ProHp is proteolytically cleaved by complement C1r subcomponent-like protein (C1r-LP) in the endoplasmic reticulum. (biomedcentral.com)
  • Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). (novusbio.com)
  • Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. (novusbio.com)
  • Even so, plants as an alternative expression platform offer unique advantages, particularly when target proteins are difficult to produce in conventional systems, require specific qualitative properties such as particular glycan profiles, and/or must be produced on a larger scale in response to urgent demand. (frontiersin.org)
  • Grain storage proteins are not only important in the aspects of germination but also, storage proteins are a valuable food source in human and animal nutrition. (intechopen.com)
  • Recombinant PvRAMA (rPvRAMA) protein was expressed and purified, and then used to immunize mice. (biomedcentral.com)
  • BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. (biolegend.com)
  • Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. (biolegend.com)
  • Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee . (biolegend.com)
  • Over the last three decades, the expression of recombinant proteins in plants and plant cells has been promoted as an alternative cost-effective production platform. (frontiersin.org)
  • Although plant systems are now gaining widespread acceptance as a platform for the larger-scale production of recombinant proteins, there is still resistance to commercial uptake. (frontiersin.org)
  • Nevertheless, some plant-derived proteins for research or cosmetic/pharmaceutical applications have reached the market, showing that plants can excel as a competitive production platform in some niche areas. (frontiersin.org)
  • Here, we discuss the strengths of plant expression systems for specific applications, but mainly address the bottlenecks that must be overcome before plants can compete with conventional systems, enabling the future commercial utilization of plants for the production of valuable proteins. (frontiersin.org)
  • There are also several platforms based on plants and plant cells, but these have not been included in the latest market studies, indicating they have not yet commanded a significant share of commercial protein production capacity. (frontiersin.org)
  • The expression of tumour necrosis factor (TNF)-α, interleukin (IL)-6, zonula occludens (ZO)-1, Beclin-1 and autophagy-related (atg) 5 mRNA was evaluated by quantitative PCR, and the expression of ZO-1, microtubule-associated proteins 1A/1B light chain 3B (LC3B) I and, LC3B II protein by Western blot. (karger.com)
  • Interestingly, the reduction of the expression of HIF-1α mRNA in VVs by MPFF was not reflected at the protein level. (ox.ac.uk)
  • In case of proteins for which it is possible to identify the secondary structure, symbols are given in the Stride classification (e.g. (edu.pl)
  • The DTT-reduced and the non-reduced protein migrate at approximately 19kDa by SDS-PAGE. (biolegend.com)
  • Guanylate-binding protein 1 (GBP1/GBP2b) is a member of the large family of IFN-γ-inducible GTPases. (researchsquare.com)
  • The pathogenesis of Allgrove syndrome (AS), another distinct clinical entity, is due to a defect in a WD-repeat regulatory protein named for alacrima-achalasia-adrenal insufficiency neurologic (ALADIN) disorder. (medscape.com)
  • The authors created all eight possible configurations, (donor and exceptor fused to the N and the C termini of the interacting proteins) for both the NanoBRET and the BRET1 experimental systems because energy transfer between donor and acceptor can be affected by the geometry of the molecules. (promegaconnections.com)
  • Main storage proteins in cereals are prolamins, and in maize prolamins are called zeins located within the endosperm in protein agglomerations called protein bodies. (intechopen.com)
  • Four main classes of zein proteins are: alpha, beta, gamma and delta zein. (intechopen.com)
  • This protein has NADH dehydrogenase activity and oxidoreductase activity. (nih.gov)