• An increase in intracellular copper causes ATP7A to move to the plasma membrane. (medscape.com)
  • In silico analysis of cellular localization predicts that the parasporins are mostly located in the cytoplasm and few in the plasma membrane but devoid of any signal peptide. (emanresearch.org)
  • Endocytosis is the vesicle-mediated process used by all cells to internalize extracellular macromolecules, plasma membrane lipids, and plasma membrane proteins ( Figure 1 ). (wormbook.org)
  • There are several endocytic pathways that utilize different mechanisms to internalize portions of the plasma membrane. (wormbook.org)
  • Many receptors then recycle to the plasma membrane either directly or indirectly via recycling endosomes. (wormbook.org)
  • Others are recycled back to the plasma membrane via the recycling pathway. (wormbook.org)
  • Clathrin and cargo molecules are assembled into clathrin-coated pits on the plasma membrane together with an adaptor complex called AP-2 that links clathrin with transmembrane receptors, concluding in the formation of mature clathrin-coated vesicles (CCVs). (wormbook.org)
  • The proton motive force is maintained by export of protons by a plasma membrane H + -ATPase, and therefore this sugar transport system requires net investment of ATP for its activity [ 20 ]. (biomedcentral.com)
  • The MHA attributes a leading role in differentiation and aging processes to the plasma membrane, undergoing inevitable, continuous alterations during the life. (antiaging-systems.com)
  • Membrane proteins perform a variety of functions vital to the survival of organisms: Membrane receptor proteins relay signals between the cell's internal and external environments. (wikipedia.org)
  • The EspA organelle is used to transfer the translocated intimin receptor (Tir), which is then inserted into the host cell membrane, where it binds to intimin, a bacterial outer membrane protein ( 9 , 10 ) and triggers the host cytoskeletal events that lead to attaching/effacing lesion formation. (cdc.gov)
  • is compatible with a role in regulating Ca 2+ channel activity or SNARE [soluble N -ethylmaleimide-sensitive factor attachment protein (SNAP) receptor] complex function in vesicle fusion. (jneurosci.org)
  • Notch is a large receptor protein with segments inside the cell and in the membrane at the surface of the cell. (sciencedaily.com)
  • Direct insertion of PMPs into peroxisomes depends on Pex3 and Pex19 8 , Pex19 being the chaperone/receptor for PMPs and Pex3 the peroxisomal docking protein for the Pex19-cargo complex. (rug.nl)
  • Understanding the proteins that regulate the 5-HT1A promoter will lead to insights on receptor regulation and provide new therapeutic targets relevant to depression, anxiety, obsessive compulsive disorder and other mental illnesses. (vdocuments.net)
  • Lipid-protein interactions and effect of local anesthetics in acetylcholine receptor-rich membranes from Torpedo marmorata electric organ. (mpg.de)
  • Association of spin-labeled local anesthetics at the hydrophobic surface of acetylcholine receptor in native membranes from Torpedo marmorata. (mpg.de)
  • The theoretical overview of this research would facilitate the researchers with valuable insights of the PS protein structures, cancer cell killing mechanism of PS-5 and PS-6 proteins eventually in tumor micro-environment and their receptor molecules with a view to develop anti-cancer drugs. (emanresearch.org)
  • Previous in vivo studies indicated that unlike the E. coli signal recognition particle (SRP), the SRP receptor FtsY is required for membrane targeting of ribosomes. (rupress.org)
  • Our results with E. coli , in conjunction with recent observations made with the yeast Saccharomyces cerevisiae , raise the possibility that the SRP receptor-mediated formation of intracellular membrane networks is governed by evolutionarily conserved principles. (rupress.org)
  • 19 normal healthy in- receptor protein (CD117) that is structurally dividuals were recruited as a control group. (who.int)
  • The subunit produced from OSMR can also pair with a different protein to form the OSM receptor type II. (medlineplus.gov)
  • OSM receptor type II interacts with a protein called oncostatin M (OSM). (medlineplus.gov)
  • Peripheral membrane proteins are temporarily attached either to the lipid bilayer or to integral proteins by a combination of hydrophobic, electrostatic, and other non-covalent interactions. (wikipedia.org)
  • The study of MscL, a highly convenient model system, will give us basic understanding and the first example of what type of intramolecular interactions, sequence motifs, and conformations make membrane proteins sensitive to a physiologically relevant mechanical stimulation. (usda.gov)
  • The combination of such self-organizational phenomena with canonical intermolecular interactions is most likely to control the release of membrane proteins from the ER into the secretory pathway. (biologists.com)
  • This process depends on the general physico-chemical features of the cargo membrane protein and on the interactions of these features with the collective properties of the bilayer, instead of the one-to-one intermolecular interactions that exist between discrete signals and their receptors. (biologists.com)
  • Import of peroxisomal membrane proteins: the interplay of Pex3p- and Pex19p-mediated interactions. (rug.nl)
  • Various experimental approaches exist for the study of nucleic acid-protein interactions in vitro and in vivo , but the detection of such interactions at the subcellular level remains challenging. (nature.com)
  • Here we describe how to detect nucleic acid-protein interactions in plant leaves by using a fluorescence resonance energy transfer (FRET) approach coupled to fluorescence lifetime imaging microscopy (FLIM). (nature.com)
  • Interaction of membrane-spanning proteins with peripheral and lipid-anchored membrane proteins: perspectives from protein- lipid interactions (Review). (mpg.de)
  • Interactions between lipid-anchored and transmembrane proteins. (mpg.de)
  • Integration of a K+ channel-associated peptide in a lipid bilayer: Conformation, lipid-protein interactions, and rotational diffusion. (mpg.de)
  • Lipid-protein interactions in ADP-ATP carrier/egg phosphatidylcholine recombinants studied by spin-label ESR spectroscopy. (mpg.de)
  • Influence of polar residue deletions on lipid-protein interactions with the myelin proteolipid protein. (mpg.de)
  • 1998, Science 282:2220-2226) provided a strong framework for the evaluation of conformations that permit opening of a large MscL pore by membrane tension. (usda.gov)
  • Secretory proteins exit the endoplasmic reticulum (ER) in coat protein complex II (COPII)-coated vesicles and then progress through the Golgi complex before delivery to their final destination. (biologists.com)
  • Peroxisomal membrane proteins insert into the endoplasmic reticulum. (rug.nl)
  • Revisiting the Latency of Uridine Diphosphate-Glucuronosyltransferases (UGTs)-How Does the Endoplasmic Reticulum Membrane Influence Their Function? (mdpi.com)
  • Uridine diphosphate-glucuronosyltransferases (UGTs) are phase 2 conjugation enzymes mainly located in the endoplasmic reticulum (ER) of the liver and many other tissues, and can be recovered in artificial ER membrane preparations (microsomes). (mdpi.com)
  • ATP2A2, located on 12q23-24.1, encodes the sarcoplasmic/endoplasmic reticulum Ca 2+ -ATP isoform 2 protein (SERCA2), which is a calcium pump. (medscape.com)
  • In a different study, in which researchers systematically analyzed mutations identical to those found in patients with Darier disease, mutant SERCA2 protein aggregates were found to cause stress to the endoplasmic reticulum, subsequently inducing cell apoptosis. (medscape.com)
  • The generated 3D models of PS proteins upon verification by Ramachandran plot analysis confirmed that our prediction lies in the good quality model range and facilitated the understanding of the very protein folding, assembly into complexes and cell killing mechanisms. (emanresearch.org)
  • Here we show that in cells depleted of the SRP protein, Ffh or the translocon component SecE, the ribosomal targeting pathway is blocked downstream and unprecedented, membrane-bound FtsY-ribosomal complexes are captured. (rupress.org)
  • The accumulation of FtsY-ribosome complexes induces the formation of intracellular membranes needed for their quantitative accommodation. (rupress.org)
  • Electron microscopy reveals loss of desmosomes (epithelial intercellular junctions formed by membrane and submembrane protein complexes), breakdown of desmosome-keratin intermediate filament attachment, and perinuclear aggregates of keratin intermediate filaments. (medscape.com)
  • citation needed] They can be classified according to their relationship with the bilayer: Integral polytopic proteins are transmembrane proteins that span across the membrane more than once. (wikipedia.org)
  • Bitopic proteins are transmembrane proteins that span across the membrane only once. (wikipedia.org)
  • Polypeptide toxins and many antibacterial peptides, such as colicins or hemolysins, and certain proteins involved in apoptosis, are sometimes considered a separate category. (wikipedia.org)
  • Alterations in mitochondrial functions such as oxidative phosphorylation (OXPHOS) and membrane polarity, which increase oxidative stress and apoptosis, may precede the development of depressive symptoms. (frontiersin.org)
  • It could be hypothesized that the PS-5 protein might induce apoptosis or act as β- pore forming toxin to kill specific cancer cells while PS-6 might act simply as pore forming toxin. (emanresearch.org)
  • In lichen and macular amyloidosis, it is thought that scratching the itchy skin causes skin cells to undergo apoptosis, releasing proteins that abnormally clump together and form amyloids. (medlineplus.gov)
  • citation needed] Integral and peripheral proteins may be post-translationally modified, with added fatty acid, diacylglycerol or prenyl chains, or GPI (glycosylphosphatidylinositol), which may be anchored in the lipid bilayer. (wikipedia.org)
  • These proteins are water-soluble but can aggregate and associate irreversibly with the lipid bilayer and become reversibly or irreversibly membrane-associated. (wikipedia.org)
  • For membrane proteins, a third mechanism, based on the interaction of their transmembrane domain (TMD) with lipid microdomains, must also be considered. (biologists.com)
  • Our study uses classical algorithms such as space partitions and Dijkstra paths search 9 , applied in an original and novel way by taking into account the full representation of a membrane protein including the solvent and the lipid bilayer (Fig. 1 ). (nature.com)
  • The FepA membrane protein is surrounded by a lipid bilayer separating the extracellular medium and the periplasm. (nature.com)
  • Solid-state NMR spectroscopy performed on the 2H-labeled Ala-56 TM-A peptide incorporated into magnetically aligned phospholipid bilayers indicated that the peptide is tilted 8 degrees with respect to the membrane normal of the lipid bilayer. (shengsci.com)
  • Thus, this study defines the location of key residues in TM-A with respect to the lipid bilayer, describes the conformation of TM-A in a biomembrane mimic, presents a peptide-bilayer model useful in the consideration of local protein folding in the microsomal desaturases, and presents a model of arginine and tyrosine control of transmembrane protein stability and insertion. (shengsci.com)
  • A number of membrane proteins act via binding at the water/lipid bilayer interface. (shengsci.com)
  • A single-residue deletion alters the lipid selectivity of a K+ channel-associated peptide in the beta-conformation: Spin label electron spin resonance studies. (mpg.de)
  • Spin label saturation transfer EPR determinations of the stoichiometry and selectivity of lipid-protein interations in the gel phase. (mpg.de)
  • Exchange rates at the lipid-protein interface of the myelin proteolipid protein determined by saturation transfer electron spin resonance and continuous wave saturation studies. (mpg.de)
  • His research is focused on the biochemistry of membrane transport proteins and the structure, function and regulation of lipoxygenases - enzymes involved in lipid metabolism and in the generation of a wide array of signaling molecules. (wsu.edu)
  • The formation of lipid rafts and cavioli in biological membranes provides examples of cooperative association of lipids presumably assisted by the presence of cholesterol acting as a lineactant in the two-dimensional system of the membrane. (lu.se)
  • The coat on the budding vesicle comprises two layers, an inner layer of adaptor proteins (gray ovals) and an outer layer that forms a polyhedral cage. (biologists.com)
  • Many receptors and their associated ligands cluster into clathrin-coated pits by association with clathrin adaptor proteins such as the four-subunit complex AP2. (wormbook.org)
  • It is estimated that 20-30% of all genes in most genomes encode for membrane proteins. (wikipedia.org)
  • Mutations in the genes that codes for presenilin lead to misprocessing of Notch and APP so that the resulting proteins either cannot do their assigned jobs properly or actively damage cells. (sciencedaily.com)
  • Two genes that code for presenilin were discovered in 1995 by investigators studying a particularly aggressive form of inherited Alzheimer's disease that strikes people as young as 30 years of age. (sciencedaily.com)
  • A number of bleeding disorders arise as a result of mutations in the genes for proteins involved in platelet aggregation. (hindawi.com)
  • With 35,000 genes and hundreds of thousands of protein states to identify, correlate, and understand, it no longer suffices to rely on studies of one gene, gene product, or process at a time. (genomicglossaries.com)
  • have begun to reveal new components and new mechanisms associated with intracellular membrane traffic in a variety of cell types. (wormbook.org)
  • Due to these membrane alterations, an accumulation of dry mass (i.e., a decrease of the intracellular water content) takes place in the intracellular space. (antiaging-systems.com)
  • In this report, we conducted mass spectrometric analysis of the N-glycosylation profiles of recombinant spike proteins derived from four common SARS-CoV-2 variants classified as Variant of Concern, including Alpha, Beta, Gamma, and Delta along with D614G variant spike as a control. (cdc.gov)
  • Membrane proteins are common proteins that are part of, or interact with, biological membranes. (wikipedia.org)
  • Such proteins can be separated from the biological membranes only using detergents, nonpolar solvents, or sometimes denaturing agents. (wikipedia.org)
  • Proteomic approaches have proven powerful at identifying large numbers of proteins, but there are fewer reports of functional characterization of proteins in biological tissues. (hindawi.com)
  • Complex biological samples have also been successfully separated in multiple dimensions at the protein level. (hindawi.com)
  • I have analyzed the contradictory items of the FRTA, outlining a new, comprehensive interpretation of the possible biological role of oxygen free radicals in the living state, cell differentiation and aging, called the membrane hypothesis of aging (MHA). (antiaging-systems.com)
  • Expanded polytetrafluoroethylene (e-PTFE) membranes have been the standard materials for clinical treatment with guided bone regeneration (GBR), achieving good results when used as mechanical barriers covering sites of extraction e- PTFE is a polymer with high stability in biological systems, which provides better tissue organization, infection resistance and no induction of inflammatory reactions 1 . (bvsalud.org)
  • Like Notch, APP is a large protein that spans the cellular membrane. (sciencedaily.com)
  • After analysing for many years the way that DNA glycosylases, APE1 and XRCC1 initiate the repair of 8-oxoG or abasic sites, our laboratory is now interested in studying the effect on BER proteins of cellular environmental changes such as oxidative stress or the switch from stem cells to differentiation. (cea.fr)
  • More recently we have also established that unexpected factors such as the cellular prion protein can stimulate BER and we are now trying to identify the underlying mechanisms and the possible physiological implications of this response. (cea.fr)
  • These proteins may modulate the interaction of platelets with their local cellular environment. (hindawi.com)
  • Rapamycin crosses cellular membranes and binds to an enzyme or protein inside cells. (newstarget.com)
  • It then sends signals throughout the cell to use these nutrients to build, grow, proliferate and make new proteins, enzymes and cellular components. (newstarget.com)
  • To facilitate uptake and conversion of glucose at low extracellular concentrations, activity of high-affinity transporters ( K M ~ 1 mM) is required, in combination with hexokinase activity to 'trap' the sugar inside the cell in its phosphorylated form and therefore maintain a glucose concentration gradient over the cellular membrane [ 15 ]. (biomedcentral.com)
  • But large- scale omic studies of cellular molecules in aggregate rarely can answer interesting questions without the assistance of information from traditional hypothesis- driven research. (genomicglossaries.com)
  • In this review, we summarize some of the latest knowledge on mitochondrial dysregulation in major depression (depicted in Figure 1 ) and also discuss how mitochondrial dysfunction could instigate downstream changes in extracellular matrix proteins such as reelin, neuronal nitric oxide (nNOS), oxidative stress, and inflammation, and finally adult hippocampal neurogenesis. (frontiersin.org)
  • 1995) Processing of the foreex precursor protein and its regu- lation in Alzheimers disease. (dpforex.com)
  • The analysis of mutations now allows us to narrow the search for functionally important regions to the short N-terminal helix, two transmembrane domains of the protein, and the loop between them. (usda.gov)
  • That work has demonstrated that presenilin mutations lead to the disproportionate formation from APP of a 42-amino-acid amyloid protein variant abundant in Alzheimer's disease plaques. (sciencedaily.com)
  • The mechanisms by which specific ATP2A2 mutations impact the function of the ATP2A2 protein have been investigated using an in vitro model. (medscape.com)
  • This study reports the solid-state NMR spectroscopic characterization of the amino-proximate transmembrane domain (TM-A) of a diverged microsomal delta12-desaturase (CREP-1) in a phospholipid bilayer. (shengsci.com)
  • Systems that previously were considered too complex for a molecular characterization now successively become available to fundamental studies. (lu.se)
  • The Menkes protein is also in retinal pigment epithelial cells and the neurosensory retina. (medscape.com)
  • This review summarizes recent work elucidating endocytic pathways, primarily in the worm germ line and coelomocytes, and also touches on diverse studies of secretion, especially in ectodermal cells of epithelial character. (wormbook.org)
  • e-PTFE membranes are used as mechanical barriers to protect the blood clot and allow bone cells to be selected to repopulate the bone defect, preventing the epithelial tissue to migrate into the defect 1 . (bvsalud.org)
  • article{westin_apical_1999, title = {Apical mitotic activity and growth in clones of {Norway} spruce in relation to cold hardiness}, volume = {29}, doi = {10/dgsxgs}, abstract = {Seasonal development of apical mitotic activity and growth in three clones of Norway spruce (Picea abies (L.) Karst. (upsc.se)
  • To illustrate our approach, we provide experimental procedures to detect the interaction between plant DNA and two proteins (the AeCRN13 effector from the oomycete Aphanomyces euteiches and the AtWRKY22 defensive transcription factor from Arabidopsis ). (nature.com)
  • However, the nature of the early contact of ribosomes with the membrane, and the involvement of FtsY in this interaction are unknown. (rupress.org)
  • Following activation by agonists such as thrombin, platelets release storage granules and membrane vesicles that contain prothrombotic (e.g., fibrinogen), mitogenic (e.g., platelet derived growth factor), immunomodulatory (e.g., neutrophil-activating peptide 2), and adhesive (e.g., platelet endothelial cell adhesion molecule) proteins. (hindawi.com)
  • In our present view, these areas are found for systems where several types of macromolecules (proteins, polysaccharides or polyelectrolytes) and/or amphiphiles interact to form a functional or destructive unit. (lu.se)
  • HeLa cells expressing the fusion proteins OGG1-DsRED (red) and XRCC1-GFP (green) were treated with KBrO3, that mainly induces 8-oxoG. (cea.fr)
  • Figure 8: GFP lifetime distribution of GFP:AeCRN13 and GFP:AeCRN13 AAA fusion proteins in plant nuclei. (nature.com)
  • 16) also bind certain proteins and, hence, have also been used as affinity adsorbents. (dpforex.com)
  • In humans, current thinking suggests that fully 30% of the genome encodes membrane proteins. (wikipedia.org)
  • Communication-deficient cells (the SKHep1 cell line) were stably transfected with a plasmid containing cDNA which encodes the major gap junction protein of rat liver, connexin32. (shengsci.com)
  • For example, proteins involved in immune response The localization of proteins in membranes can be predicted reliably using hydrophobicity analyses of protein sequences, i.e. the localization of hydrophobic amino acid sequences. (wikipedia.org)
  • Computational investigation were performed to exploit their physicochemical characteristics, structural properties including three dimensional (3D) model, model quality analysis, phylogenetic assessment and functional analysis along with the cancer-cell killing mechanism of PS-5 and PS-6 proteins using standard tools of bioinformatics. (emanresearch.org)
  • Structural and functional insights of anti-cancer proteins from Bacillus thuringiensis . (emanresearch.org)
  • We propose that in the absence of a functional SRP or translocon, ribosomes remain jammed at their primary membrane docking site, whereas FtsY-dependent ribosomal targeting to the membrane continues. (rupress.org)
  • Nonetheless, compared to other classes of proteins, determining membrane protein structures remains a challenge in large part due to the difficulty in establishing experimental conditions that can preserve the correct conformation of the protein in isolation from its native environment. (wikipedia.org)
  • In their absence, the bulk of the matrix proteins are mislocalized to the cytosol 5 , even though these cells still contain peroxisomal membrane structures to which peroxisomal membrane proteins (PMPs) are sorted 6 . (rug.nl)
  • Such membrane structures were assumed to be absent in pex3 and pex19 mutant cells and, consequently, it was proposed that Pex3 and Pex19 are involved in peroxisomal membrane formation 1,7 . (rug.nl)
  • Both fundamentally different models explain why cells lack peroxisomal membrane structures in the absence of Pex3 or Pex19: in model A, PMPs are instable or mislocalized to the cytosol in pex3 or pex19 cells, whereas in model B, PMPs accumulate at the ER. (rug.nl)
  • A comparative study of peroxisomal structures in Hansenula polymorpha pex mutants. (rug.nl)
  • A series of TM-A peptides were synthesized with 2H-labeled side chains (Ala-53, -56, and -63, Leu-62, Val-50), and their dynamic properties were studied in 1,2-dimyristoyl-sn-glycero-3-phosphatidylcholine (DMPC) bilayers at various temperatures. (shengsci.com)
  • Membrane enzymes may have many activities, such as oxidoreductase, transferase or hydrolase. (wikipedia.org)
  • The mobility of a fluorescent protein fusion can be assessed using a technique known as fluorescence recovery after photobleaching (FRAP). (microscopyu.com)
  • Figure 3: GFP fluorescence observed in Nicotiana leaves that express GFP-tagged proteins. (nature.com)
  • A list of publications describing Oryx, theoretical studies using Oryx and New methods. (douglas.co.uk)
  • We are also developing methods to identify ligands for non-druggable protein surfaces. (stanford.edu)
  • Given the tight relation between protein structure and function, we present a set of methods to analyze protein topology, implemented in the VLDP program, relying on Laguerre space partitions built from series of molecular dynamics snapshots. (nature.com)
  • The present work deals with the preparation of ultrafiltration (UF) and microfiltration (MF) membranes with enhanced fouling resistant ability by using two different methods, liquid- induced phase separation (LIPS) and vapor-induced phase separation (VIPS), after in-situ modification of the PVDF solution using amphiphilic PS-b-PEGMA block copolymers as additive. (ncl.edu.tw)
  • A meta-analysis of 22 studies of bacterial contamination rate estimates for apheresis (AP), platelet-rich plasma (PRP), and buffy coat (BC) collection methods found an overall mean contamination rate of 0.51 per 1000 components (95% confidence index [CI], 0.38-0.67). (medscape.com)
  • The so called ELISA test was used to assess protein adsorption from single protein solution (FN, γ-globulin, HSA) and PPP solution. (ncl.edu.tw)
  • Previous proteomic studies of intact platelets have collectively identified hundreds of proteins using a variety of fractionation strategies including 2-dimensional electrophoresis (2DE), multidimensional chromatographic separations, membrane prefractionation techniques, and adsorption to combinatorial hexapeptide ligand libraries [ 1 - 6 ]. (hindawi.com)
  • EspB, a protein thought to be involved in pore formation, is transferred to the host cell by this conduit and is found in both the host cell membrane and cytosol ( 7 ). (cdc.gov)
  • Interestingly, all the proteins involved in BER are encoded by the nuclear genome and the mechanism responsible for the targeting of the translated protein to the nucleus or to the mitochondria have not been clearly established yet. (cea.fr)
  • Motion, G.B., Howden, A.J.M., Huitema, E. & Jones, S. DNA-binding protein prediction using plant specific support vector machines: validation and application of a new genome annotation tool. (nature.com)
  • EspB, with the aid of a second membrane lytic protein, EspD, forms pores in the host cell as part of the translocation mechanism ( 8 ). (cdc.gov)
  • We are studying the mechanism of viral membrane fusion and its inhibition by drugs and antibodies. (stanford.edu)
  • Different mechanism including alternative splicing, alternative transcription start or dual initiation of translation, have been proposed for different BER proteins. (cea.fr)
  • At the site of bacterial attachment, the host cell membrane forms a pedestal-like structure. (cdc.gov)
  • It inhibits bacterial cell wall synthesis by binding to 1 or more of the penicillin-binding proteins. (medscape.com)
  • The worm benefits from many of the advantages of yeast as a genetically tractable organism for these kinds of studies while offering the unique opportunity to probe how these pathways have been extended and modified in the context of a multicellular animal undergoing development to produce diverse cell types such as muscles, nerves, and polarized epithelia. (wormbook.org)
  • Transport proteins move molecules and ions across the membrane. (wikipedia.org)
  • At several steps in its original production and in its signaling activity, the Notch protein is cut by other molecules in a process as yet little understood. (sciencedaily.com)
  • Transmembrane helices from these proteins have significantly different amino acid distributions to transmembrane helices from polytopic proteins. (wikipedia.org)
  • We also propose that stretch in the membrane increases the tilt of the M1 and M2 helices, making the barrel wider. (usda.gov)
  • Secretion of LEE proteins is triggered by close contact with host cells. (cdc.gov)
  • In yeast, de novo peroxisome formation was generally studied in peroxisome-deficient pex3 cells following reintroduction of the PEX3 gene. (rug.nl)
  • The Menkes protein is synthesized as a single-chain polypeptide localized to the trans-Golgi network in cells. (medscape.com)
  • DNA-binding proteins (DNA-BPs) and RNA-binding proteins (RNA-BPs) have critical roles in living cells in all kingdoms of life. (nature.com)
  • Proteins of interest (POI) are tagged with a GFP and transiently expressed in plant cells to serve as donor fluorophore. (nature.com)
  • Parasporins (PS), parasporal inclusion proteins from Bacillus thuringiensis , possess specific cytotoxicity against different cancer cells which has suggested them to be potential for cancer treatment due to their specific binding to cancer cells. (emanresearch.org)
  • Role of ribosomal protein L12 in gonococcal invasion of Hec1B cells. (rochester.edu)
  • In nodular amyloidosis, the amyloids are composed of abnormal versions of proteins called immunoglobulin light chains released from certain blood cells called plasma cells. (medlineplus.gov)
  • The investigators found that the resultant transfected cells showed defects in ATP2A2 protein expression (15 mutants), ATP hydrolysis (29 mutants), calcium transport (4 mutants), and calcium binding and kinetics (3 mutants). (medscape.com)
  • Rapid and comprehensive analysis of putative proteinous allergens ( allergenome ) by applying such a proteomic strategy … With allergenomics, we can not only detect and assign the putative allergens (proteins specifically interacting with IgE antibodies in a patient's blood) in a short time, but also analyze the quantitative and qualitative change of the antigens, depending on the surroundings and environmental conditions of an allergenic causative. (genomicglossaries.com)
  • Here we examine whether such categorisation of protein and/or chemical respiratory allergens is realistic and/or feasible. (cdc.gov)
  • There has been controversy over the last 50 years in the UGT field with respect to the explanation for the phenomenon of latency: full UGT activity revealed by chemical or physical disruption of the microsomal membrane. (mdpi.com)
  • Some of our studies are aimed at creating an HIV vaccine that elicits antibodies against a transient, but vulnerable, intermediate in the membrane-fusion process, called the pre-hairpin intermediate. (stanford.edu)
  • One important model for mechanistic studies of endocytosis in C. elegans focuses on oocytes, which internalize huge quantities of yolk proteins and their associated lipids by clathrin-mediated endocytosis ( Grant and Hirsh, 1999 ). (wormbook.org)
  • Trafficking of yolk and yolk receptors also depends critically upon the activities of the endocytic Rab proteins RAB-5 , RAB-7 , and RAB-11 , known modulators of endocytosis in all eukaryotes ( Grant and Hirsh, 1999 ). (wormbook.org)
  • Previous studies suggest that the vesicular cysteine-string protein (CSP) may modulate presynaptic Ca 2+ channel activity in fast neurotransmitter release. (jneurosci.org)
  • the most important are protein synthesis-inhibiting toxins: Shiga toxin 1 (Stx1) and 2 (Stx2). (cdc.gov)
  • Identification of a conserved protein involved in anaerobic unsaturated fatty acid synthesis in Neiserria gonorrhoeae: implications for facultative and obligate anaerobes that lack FabA. (rochester.edu)
  • It is particularly evident in the erythrocytes where de-novo protein synthesis does not take place. (antiaging-systems.com)
  • Some studies of keratosis follicularis (Darier disease) have suggested that alterations in calcium regulation may affect the synthesis, folding, or trafficking of desmosomal proteins. (medscape.com)
  • Integral membrane proteins are a permanent part of a cell membrane and can either penetrate the membrane (transmembrane) or associate with one or the other side of a membrane (integral monotopic). (wikipedia.org)
  • The lesions are characterized by localized destruction of brush border microvilli and intimate adhesion of the bacterium to the host cell membrane. (cdc.gov)
  • Fluorescent protein fusions are the tags of choice for use in FRAP because they can be bleached without detectable damage to the cell. (microscopyu.com)
  • Moreover, the sugar-negative platform strain constructed in this study acts as a valuable starting point for future studies on sugar transport or development of cell factories requiring specific sugar transport mechanisms. (biomedcentral.com)
  • In the present study, antiviral activity of four types of bioflavonoid against dengue virus type -2 (DENV-2) in Vero cell was evaluated. (who.int)
  • Our data reveal that the amino acid substitutions and deletions between variants impact the abundance and type of glycans on glycosylation sites of the spike protein. (cdc.gov)
  • Among the human diseases in which membrane proteins have been implicated are heart disease, Alzheimer's and cystic fibrosis. (wikipedia.org)
  • Researchers at the University of Pennsylvania Medical Center and other institutions have uncovered a biochemical connection between presenilin, a molecule involved in the development of Alzheimer's disease, and another protein that controls crucial aspects of developmental biology. (sciencedaily.com)
  • The hope is that work in Alzheimer's disease will help inform people studying aspects of developmental biology about what might be going on in their neck of the woods and vice versa," says Mark E. Fortini, Ph.D., an assistant professor of genetics and senior author on one of the papers. (sciencedaily.com)
  • Misprocessed APP is thought to produce the variant amyloid protein that dominates the plaques characteristic of Alzheimer's disease. (sciencedaily.com)
  • Membrane-bound ribosomes in E. coli were extensively studied over 20 years ago. (rupress.org)
  • Since then, rhGH has been administered to tens of thousands of children worldwide, making it one of the most extensively studied therapies in the pediatric pharmacopoeia. (medscape.com)
  • Anna is a Professor at the Division of Physical Chemistry at Lund University specializing on biocolloids studied by scattering techniques. (lu.se)
  • The methodology will include site-directed mutagenesis, covalent protein modifications, patch- clamp measurements, thermodynamic and kinetic analysis of dose-response curves and rates of transitions, videomicroscopy, and molecular modeling. (usda.gov)
  • Here, we describe an experimental approach that fractionates proteins released from human platelets, linking bioassay activity to identity. (hindawi.com)
  • New WSU research has pinpointed a protein that appears to play a role in how a harmful bacteria establishes itself in ticks before being transmitted to human hosts. (wsu.edu)