MAIN OUTCOME MEASURE: Germline variants at any site in the coding sequence, splice junctions, 5' untranslated region, or 3' untranslated region of the BRCA1 gene were analyzed in cases, and selected variants were analyzed in controls. (duke.edu)
The 5' promoter region, all 26 exons, their immediate 5' and 3' flanking splice junctions and the 3' untranslated region of the FVIII gene were amplified (including 16 different segments of exon 14) using GC-clamped primers. (hku.hk)
Gene14
The human prohibitin gene, located on the BRCA1 chromosome region 17q21, was originally thought to be a negative regulator of cell proliferation and a tumor suppressor. (wikipedia.org)
This anti-proliferative activity was later attributed to the 3' UTR of the PHB gene, and not to the actual protein. (wikipedia.org)
A common genetic variation in the 3'-untranslated region of the prothrombin gene is associated with elevated plasma prothrombin levels and an increase in venous thrombosis. (medscape.com)
In a TaqMan-based real-time reverse transcription PCR (RT-PCR), genomic (5′-GCCAATGCCCTGCACTTT-3′) and reverse (5′-TCCCGAGGAGGGTTTCCA-3′) primers amplify part of the nonstructural protein 5 (NS5) gene region of USUV between nt positions 9721 and 9795 (according to the USUV complete genome sequence, GenBank accession no. (cdc.gov)
3] Mutation at position 20210 of the gene results in thrombophilia. (medscape.com)
[12] The most common ones are factor V Leiden (a mutation in the F5 gene at position 1691) and prothrombin G20210A , a mutation in prothrombin (at position 20210 in the 3' untranslated region of the gene). (ipfs.io)
The 3' regulatory regions have a great diversity of cis -regulatory elements directly involved in polyadenylation, stability, transport and mRNA translation, essential to achieve the desired levels of gene expression. (frontiersin.org)
Although a small number of 3' regulatory regions have been identified and validated so far, many studies have shown that plant 3' regulatory regions have a higher potential to regulate gene expression in plants compared to widely used 3' regulatory regions, such as NOS and OCS from Agrobacterium tumefaciens and 35S from cauliflower mosaic virus. (frontiersin.org)
In this review, we discuss the role of 3' regulatory regions in gene expression, and the superior potential that plant 3' regulatory regions have compared to NOS, OCS and 35S 3' regulatory regions. (frontiersin.org)
5'-untranslated region (5'UTR), the hypervariable region 1 (HVR1), non-structural gene 5a (NS5a) and non-structural gene 5b (NS5b). (cdc.gov)
Gain-of-function alleles of the Drosophila gene Bearded ( Brd ) cause sensory organ multiplication and loss pheno-types indistinguishable at the cellular level from those caused by loss-of-function mutations in the genes of the Notch pathway (Leviten, M. W. and Posakony, J. W. (1996). (biologists.com)
Moreover, the 3′ UTRs of Brd and of the m4 transcription unit of the Enhancer of split gene complex [E(spl)-C] exhibit an unusually high degree of sequence identity that includes not only Brd box sequences but also a second motif we refer to as the 'GY box' (GTCTTCC). (biologists.com)
MiRNAs typically bind to the 3′ untranslated region (UTR) of their mRNA targets and downregulate gene expression via mRNA degradation or translational inhibition ( 8 - 10 ). (spandidos-publications.com)
30) in the SEDL gene from Xp22 region. (nature.com)
Tanguay1
R. L. Tanguay and Gallie, D. R. , " Isolation and characterization of the 102-kilodalton RNA-binding protein that binds to the 5′ and 3′ translational enhancers of tobacco mosaic virus RNA " , Journal of Biological Chemistry , vol. 271, pp. 14316-14322, 1996. (oregonstate.edu)
Hypervariable region1
Next generation sequencing (NGS) of the hypervariable region (HVR1) of HCV and Global Hepatitis Outbreak and Surveillance Technology (GHOST) were used to determine HCV genotype, genetic heterogeneity, and construct transmission networks. (cdc.gov)
MRNA8
The effect of increasing the length of the 3'-untranslated region (UTR) on expression of luciferase mRNA was examined in transiently transfected carrot protoplasts. (oregonstate.edu)
The effect of the 3'-UTR on both poly(A)- and poly(A)+ mRNA was examined. (oregonstate.edu)
For poly(A)- mRNA, there was a consistent increase in expression when the length of the 3'-UTR was increased from 4 to 104 bases. (oregonstate.edu)
For poly(A)+ mRNA, expression increased 18-fold when the length of the 3'-UTR was increased from 7 to 27 bases. (oregonstate.edu)
These data suggest that the length of the 3'-UTR plays an important role in determining the extent to which a poly(A) tail can stimulate the translation of an mRNA. (oregonstate.edu)
Smaug, a protein that helps to establish a morphogen gradient in Drosophila embryos by repressing the translation of nanos (nos) mRNA, binds to the 3' untranslated region (UTR) of nos mRNA via two similar hairpin structures. (embl.de)
The presence, absence, abundance and activity of splicing factors can effect which regions of the pre-mRNA will be included in the mature mRNA. (berkeley.edu)
They found that one round of amplification produced approximately 10 3 fold of the estimated amount of starting mRNA, and two rounds produced an approximately 10 5 fold increase. (genomicglossaries.com)
Venous thromboembolism2
First reported in 1996 as a familial cause of venous thromboembolism, the prothrombin 20210a mutation results in increased levels of plasma prothrombin and a concurrent increased risk for the development of thrombosis. (medscape.com)
Heterozygous mutations raise the risk of venous thromboembolism about 3-fold to 11-fold. (medscape.com)
Lengths2
Prohibitin is expressed as two transcripts with varying lengths of 3' untranslated region. (wikipedia.org)
Site(s) of transcription initiation and lengths of 5′- and 3′-untranslated regions were determined with 5′- and 3′-RACE. (elsevierpure.com)
Mutation1
Individuals carrying the prothrombin 20210a mutation have a 2- to 3-fold increased risk for developing thrombosis. (medscape.com)
The role of the 3′-untranslated region of non-polyadenylated plant viral mRNAs in regulating translational efficiency. (microbiologyresearch.org)
In addition, after transcription, a wide array of RNA-binding proteins interacts with cis -acting elements located mainly in the 3' untranslated region, determining the fate of mRNAs in eukaryotic cells. (frontiersin.org)
Transcripts1
The availability of multiple PAS in the same 3' regulatory region enables the inclusion or exclusion of 3' untranslated region (3'UTR) sequences, resulting in transcripts that may differ in particularities involving post-transcriptional processes such as stability, transport and translation ( Mayr, 2016 ), and even protein localization ( Berkovits and Mayr, 2015 ). (frontiersin.org)
Sequence1
A nested set of constructs in which the 3'-UTR increased from 4 to 104 bases was generated by the introduction and reiteration of a 20 base sequence downstream of the luc stop codon. (oregonstate.edu)
Significantly2
In black women, a variant in the 3' untranslated region was statistically significantly more common in cases than in controls. (duke.edu)
miR-195 level was dynamically regulated in response to MI and significantly increased in ischemic regions 24 h post-MI as well as in hypoxic or H 2 O 2 -treated cardiomyocytes. (ijbs.com)
Amplification2
The TaqMan probe (FAM-5′-CGATGTCCAAGGTCAGAAAAGACGTGC-3′-TAMRA) hybridizes the amplification product between nt positions 9746 and 9773. (cdc.gov)
Hepatitis C RNA: Testing for HCV RNA by reverse-transcriptase ¬polymerase-chain reaction (RT-PCR) amplification of the 5' noncoding region was performed on anti-HCV positive samples. (cdc.gov)
Regulatory1
The longer transcript is present at higher levels in proliferating tissues and cells, suggesting that this longer 3' untranslated region may function as a trans-acting regulatory RNA. (wikipedia.org)
Primers1
The initial PCR reaction amplifies a region of the HCV genome (External primers). (cdc.gov)
Plants1
The effect of the length of the 3'-untranslated region on expression in plants. (oregonstate.edu)
Journal1
Journal of Investigative Medicine , 44 (3), 253a. (elsevierpure.com)
Blood2
Explain to the blood that the youth last will and testament necessary to stay the pediatric endocrinologist every 3 to 6 months to track for growth, against potential adverse effects, and an eye to compliance with therapy. (mein-schoenefeld.info)
We find that the Brd transcript is truncated and accumulates to substantially higher levels in the gain-of-function mutants, due to the insertion of a transposable element of the blood family in the Brd 3′ untranslated region (UTR). (biologists.com)
Role1
The present study was designed to investigate the role of the miR‑133b‑5p in HPC‑induced cardioprotection and the underlying mechanisms involving caspase‑8 and caspase‑3 apoptotic signaling. (spandidos-publications.com)
Mutations1
She was taking com- ase (MTHFR) point mutations [ 3 ]. (who.int)
Present1
We find that both the Brd box and the GY box are also present in the 3′ UTRs of several basic helix-loop-helix repressor-encoding genes of the E(spl)-C, often in multiple copies, suggesting that a novel mode of post-transcriptional regulation applies to Brd and many E(spl)-C genes. (biologists.com)
Virus2
Retrospective analysis of archived tissue samples from bird deaths in the Tuscany region of Italy in 1996 identified Usutu virus. (cdc.gov)
A particular strain of Usutu virus (USUV) was determined as the causative agent of this fatal bird disease outbreak, which recurred in Austria in subsequent years ( 3 ), and was later identified in Hungary ( 4 ), Switzerland ( 5 ), Italy ( 6 ), and Germany ( 7 ). (cdc.gov)
Table1
The radiographic manifestations of SEDL are diagnostic, clearly distinguishing it from other conditions that it has most recently been grouped together within the current classification 3 (see Table 1 ). (nature.com)
Germany1
The Gutenberg Health Study (GHS) is a prospective, observational, single center cohort study, designed for population-based health research, in the Rhine-Main region in Germany. (biomedcentral.com)
Multiple1
Alternative splicing is the process whereby a single genetic locus can be transcribed and processed to generate multiple, distinct isoforms[ 1 - 3 ]. (berkeley.edu)
Risk1
[3] A significant proportion of the population has a detectable abnormality, but most of these only develop thrombosis in the presence of an additional risk factor. (ipfs.io)
Positive1
Vincristine binds to β-tubulin subunits at the positive end of microtubules at a region called the _Vinca_-binding domain. (smpdb.ca)
Patients1
Une étude a été menée dans des unités de soins intensifs en chirurgie pour adultes de l'hôpital universitaire de Zagazig, (Égypte) auprès de 25 patients atteints de septicémie, de 27 patients atteints d'une septicémie sévère et de 28 témoins. (who.int)