• 31,000 5' expressed sequence tags (ESTs) derived from four trophozoite libraries generated from two P. marinus strains. (biomedcentral.com)
  • Among the matched sequences, ESTs were identified from tissues other than berries or from berries at different developmental stages. (biomedcentral.com)
  • Additional MPSS signatures not matching to known grape ESTs can extend our knowledge of the V. vinifera transcriptome, particularly when these data are used to assist in annotation of whole genome sequences from Vitis vinifera . (biomedcentral.com)
  • Here, we describe the isolation and characterization of expressed sequence tags (ESTs) isolated from a chicken pineal gland cDNA library. (westminster.ac.uk)
  • 6% of the ESTs matched neither GenBank cDNA sequences nor the newly assembled chicken genomic DNA sequence, three ESTs aligned with sequences designated to be on the Z_random, while one matched a W chromosome sequence and could be useful in cataloguing functionally important genes on this sex chromosome. (westminster.ac.uk)
  • Additionally, single nucleotide polymorphisms (SNPs) were identified and validated in 10 ESTs that showed 98% or higher sequence similarity to known chicken genes. (westminster.ac.uk)
  • 2006. Evaluating genetic diversity of Iranian alfalfa local populations using expressed sequence tags (ESTs) microsatellites. (innspub.net)
  • 2001). Exhaustive sequencing of portrayed series tags (ESTs) was the initial technique SB-408124 used for speedy identification of portrayed genes and gene appearance profiling (Adams et al. (colinsbraincancer.com)
  • Thus, in one embodiment, peptides comprising the sequences described in SEQ ID NOs. (justia.com)
  • Expressed sequence tag (EST) sequencing projects have generated millions of cDNA sequences for human, mouse and other organisms that are identified by an accession number in databases such as GenBank. (biomedcentral.com)
  • In general, a sequence-specific identifier (GenBank accession number) serves as a reference to the array probe sequence. (biomedcentral.com)
  • To generate Tg(CerPrP-E226) mice expressing the elk PrP coding sequence, codon 226 of the deer PrP gene ( PRNP ) (GenBank accession no. (cdc.gov)
  • Thus, this protein is expressed in a cell cycle-dependent manner during liver regeneration, and is thought to have an important role in the regulation of cytokinesis. (wikipedia.org)
  • Similarity searches revealed that 55% of these had hits in protein sequence databases, of which 1,729 had their best hit with proteins from the chromalveolates (E-value ≤ 1e-5). (biomedcentral.com)
  • Some sequences are similar to those proven to be targets for effective intervention in other protozoan parasites, and include not only proteases, antioxidant enzymes, and heat shock proteins, but also those associated with relict plastids, such as acetyl-CoA carboxylase and methyl erythrithol phosphate pathway components, and those involved in glycan assembly, protein folding/secretion, and parasite-host interactions. (biomedcentral.com)
  • Purification of a histidine-tagged NnrS demonstrated that NnrS is a haem- and copper-containing membrane protein. (microbiologyresearch.org)
  • Protein motif recognition in DNA sequences containing indel errors. (auth.gr)
  • Mining of Assembled Expressed Sequence Tag (EST) Data for Protein Families: Application to the G Protein-coupled Receptor Superfamily. (auth.gr)
  • When expressed as a fusion protein with eYFP in cultured cells, Sec31B-F associates with the endoplasmic reticulum and with vesicular-tubular clusters, displays restricted intracellular movement characteristic of COPII vesicle dynamics, co-distributes on organelles with Sec13, Sec31A and Sec23 (markers of the COPII coat), and concentrates with ts045-VSV-G-CFP (VSV-G) when examined early in the secretory pathway or after temperature or nocodazole inhibition. (biologists.com)
  • Sequence comparison does not support an evolutionary link between halobacterial retinal proteins including bacteriorhodopsin and eukaryotic G-protein-coupled receptors. (springer.com)
  • The fact that CWD prion incubation times in transgenic mice expressing elk prion protein are consistently more rapid raises the possibility that residue 226, the sole primary structural difference between deer and elk prion protein, may be a major determinant of CWD pathogenesis. (cdc.gov)
  • Bioassays in Tg mice expressing deer prion protein (PrP) ( 4 ) and newly created Tg mice expressing elk PrP, demonstrated low levels of CWD prions in antler velvet. (cdc.gov)
  • Danio pol β encoded by the in vitro / in vivo -compatible pIVEX plasmid was expressed in E. coli BL21(DE3), BL21(DE3)pLysS, and KRX, and in vitro as a C-terminal His-tagged protein. (biomedcentral.com)
  • 1999). Winter flounder expressed sequence tags: Establishment of an EST database and identification of novel fish genes. (geneticsmr.com)
  • Further, although P. marinus sequences display significant similarity to those from both apicomplexans and dinoflagellates, the presence of trans-spliced transcripts confirms the previously established affinities with the latter. (biomedcentral.com)
  • They were characterized as a new member of the thionine family due to their similarity in molecular mass, amino acid sequence and number of cysteines. (frontiersin.org)
  • These tag-based technologies, which do not require a priori knowledge of genomic sequence, are well-suited for transcriptional profiling. (biomedcentral.com)
  • These processes have left footprints in the genomic sequence that can be detected by bioinformatic methods and may help to interpret receptor function in the light of a given species in its environment. (springer.com)
  • Deletion analysis indicated the sequence 5′-TTGCG(N 4 )CACAA-3′, which is similar to sequences found in nirK and nor , is required for nnrS expression. (microbiologyresearch.org)
  • Mutation of this sequence to the consensus Fnr-binding sequence by changing two bases in each half site caused nnrS expression to become nitrate independent. (microbiologyresearch.org)
  • Global gene-expression analysis is carried out using different technologies that are either array- or sequence-tag-based. (biomedcentral.com)
  • Quantitative PCR and high-throughput methods, such as expressed sequence tag analysis, have been used for measuring gene expression and identifying subsets of elm genes that are differentially expressed in the presence of O. novo-ulmi . (sisef.it)
  • In the vertebrate CNS, HB9/MNR2 transcription factors are specifically expressed in all somatic motoneurons and are necessary to distinguish motoneurons from interneurons, in part by repressing interneuron-specific gene expression. (jneurosci.org)
  • Mouse HB9 maintains expression in somatic motoneurons, whereas chick HB9 is expressed only in postmitotic somatic motoneurons. (jneurosci.org)
  • The coding sequence was inserted into the MoPrP.Xho expression vector, and the purified transgene was microinjected into pronuclei of fertilized FVB/ Prnp 0/0 oocytes. (cdc.gov)
  • We used the in vitro / in vivo dual expression system plasmid, pIVEX, to express Danio rerio pol β ( Danio pol β) for biochemical characterization. (biomedcentral.com)
  • 1995). It really is an effective incredibly, effective, and global strategy for examining gene expression information, novel gene breakthrough, revealing book pathways, and metabolic circuits. (colinsbraincancer.com)
  • Over-expressed MS1 co-localized with actin in H9c2 cells and altered expression of genes of the myocardin-related transcription factor (MRTF)/serum response factor (SRF) transcriptional pathways and in addition the apoptosis repressor with caspase recruitment domain (Nol3) gene. (westminster.ac.uk)
  • [ 2 , 3 ] Another study indicates that the T lymphocyte subset in congenital aplasia may be associated with presence of CD4(+) T cells that express the "naïve" phenotype, possible failure in CD8(+) cytotoxic effectors differentiation and tendency to the proinflammatory status of cells, low interleukin (IL) 10 expression, and suboptimal lymphocyte responses to mitogenic stimulation. (medscape.com)
  • Genome sequencing is now an rising technology for large-scale gene breakthrough, and several prokaryotic and eukaryotic genomes have already been sequenced within the last couple of years completely. (colinsbraincancer.com)
  • Until recently, however, functional analysis of the DED fungi was hampered by the lack of protocols for efficient gene knockout and by the unavailability of a full genome sequence. (sisef.it)
  • Development, characterization and cross species amplification of polymorphic microsatellite markers from expressed sequence tags of turmeric ( Curcuma longa L.) Molecular Biotechnology. (spices.res.in)
  • SAGE results in quantitative information connected to 10-14 base-pair (bp) sequence tags derived from the 3' ends of transcripts. (biomedcentral.com)
  • The sequence depth of MPSS allowed us to capture and quantify almost all the transcripts at a specific stage in the development of the grape berry. (biomedcentral.com)
  • The number and relative abundance of transcripts from stage II grape berries was defined using Massively Parallel Signature Sequencing (MPSS). (biomedcentral.com)
  • Nevertheless, microarrays are at the mercy of inherent limitations, such as for example history intensities that could rival indicators for portrayed transcripts weakly, the issue of distinguishing between carefully related sequences (Duggan et al. (colinsbraincancer.com)
  • Based on primary sequence comparisons, β subunits are predicted to be modular structures composed of five domains (A-E) that are related to the large family of membrane-associated guanylate kinase proteins. (jneurosci.org)
  • Based on oil accumulation dynamics of V. galamensis seeds, we harvested seed samples from three stages (17, 38, and 45 days after pollination, DAP) representing the initial, fast and final EFA accumulation phases, and one mixed sample from different tissues for RNA-sequencing, with three biological replicates at each sample. (researchsquare.com)
  • 1995). This technique consists of the large-scale, single-pass, and incomplete sequencing of cDNA clones (around 500 bp), from a lot of libraries representing diverse tissues usually. (colinsbraincancer.com)
  • These analyses have also helped identify genes that were differentially expressed in DED fungi grown under defined experimental conditions. (sisef.it)
  • Here, we have described resources that may be useful in comparative and functional genomic analysis of genes expressed in an important organ, the pineal gland, in a model and agriculturally important organism. (westminster.ac.uk)
  • Drosophila genes expressed specifically in all motoneurons have not been described, although a growing number of genes are known to be expressed in subsets of motoneurons. (jneurosci.org)
  • Here we performed mRNA profiling using Massively Parallel Signature Sequencing (MPSS) and combined it with available Expressed Sequence Tag (EST) data. (biomedcentral.com)
  • Despite the low level of amino acid sequence identity between defensins, their three dimensional structures are remarkably similar between different plant defensins ( Pelegrini and Franco, 2005 ). (frontiersin.org)
  • In another embodiment, serine-67 is present in a peptide of less than 30 amino acids that comprises the sequence of SEQ ID NO. 4. (justia.com)
  • In another embodiment, the peptide comprising the sequence of SEQ ID NO. 4 is less than 25 amino acids in length. (justia.com)
  • In a more preferred embodiment, the peptide comprising the sequence of SEQ ID NO. 4 is 18 amino acids long. (justia.com)
  • http://dx.doi.org/10.1093/nar/7.6.1513 PMid:388356 PMCid:342324 Boldman KG, Kriese LA and Van Vleck LD (1995). (geneticsmr.com)
  • http://dx.doi.org/10.1093/molbev/msp094 PMid:19420050 PMCid:2734151 Doudna JA and Rath VL (2002). (geneticsmr.com)
  • http://dx.doi.org/10.1083/jcb.123.6.1631 PMid:8276885 Hale LP, Patel DD, Clark RE and Haynes BF (1995). (geneticsmr.com)
  • 2003. Comparative analyses of potato expressed sequence tag libraries . (berkeley.edu)
  • Using this process, you can generate 2-3 libraries, each that contains over 4.5 million tags, within a full month. (colinsbraincancer.com)
  • Association of nucleotide patterns with gene function classes: application to human 3' untranslated sequences. (auth.gr)
  • AN - check the tag INFANT HN - 2008 FX - Child Nutrition FX - Infant Nutrition Physiology FX - Milk FX - Milk, Human DH - Adolescent Nutrition DI - 052508 MN - SP6.021.067 MS - Nutrition of persons 10 through 19 years of age. (bvsalud.org)
  • For additional information on NCHS data products, contact the: Data Dissemination Branch, NCHS 6525 Belcrest Rd. Hyattsville, MD 20782 Tel: (301) 436-8500 E-mail: [email protected] Internet: http://www.cdc.gov/nchswww/nchs/home.htm I. DESCRIPTION OF THE NATIONAL AMBULATORY MEDICAL CARE SURVEY A. INTRODUCTION This file contains data collected in the 1995 National Ambulatory Medical Care Survey (NAMCS). (cdc.gov)
  • Trimming and clustering of the sequence tags yielded 7,863 unique sequences, some of which carry a spliced leader. (biomedcentral.com)
  • Because no unique gene nomenclature or sequence identifier is used for all platforms, a database linking the different identifiers belonging to one gene and its orthologs is required. (biomedcentral.com)
  • The EST analysis reported herein, together with the recently completed sequence of the P. marinus genome and the development of transfection methodology, should result in improved intervention strategies against dermo disease. (biomedcentral.com)
  • In 1995, a total of 36,875 Patient Record forms were received from the 1,883 physicians who participated in the NAMCS. (cdc.gov)
  • METHODS: To establish the prevalence of VAPB mutations, we screened 80 familial ALS samples by DNA sequencing. (bvsalud.org)
  • He thanked the regional and global specialized laboratories for their efforts to provide timely results of the genomic sequencing of wild polioviruses, and for their help in planning and monitoring polio eradication activities in the Region. (who.int)
  • Here, we show that vinculin null (vin −/− ) cells and cells expressing a vinculin Y822F mutant have increased survival due to up-regulated activity of extracellular signal-regulated kinase (ERK). (rupress.org)
  • check the tag ADOLESCENCE HN - 2008 BX - Nutrition in Adolescence FX - Adolescent Nutrition Physiology MH - Peritoneal Stomata UI - D054048 MN - A01.047.025.600.700 MN - A10.810 MS - Natural openings in the subdiaphragmatic lymphatic plexus in the PERITONEUM, delimited by adjacent mesothelial cells. (bvsalud.org)
  • Specific signal sequences, AREs for example[ 17 ], can affect the rate at which the poly-adenosine tail is shortened. (berkeley.edu)
  • In addition, the database can be used to annotate array probes with reference sequences and updated gene descriptions from UniGene. (biomedcentral.com)
  • 5, 2002, which are all incorporated by reference in their entirety. (justia.com)
  • In vertebrates, chick MNR2 and mouse HB9 are expressed in presumptive somatic motoneuron progenitors. (jneurosci.org)
  • check also tag CHILD or specific HN - 2008 FX - Adolescent Nutrition FX - Infant Nutrition DH - Maternal Nutrition DI - 052505 MN - SP6.021.072 MS - Nutrition of a mother which affects the health of the INFANT as well as herself. (bvsalud.org)