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  • Embryo
  • Moreover, culturing SCNT embryos derived from mtDNA depleted cells in media supplemented with a nuclear reprograming agent can increase the levels of expression of genes related to embryo development when compared with non-depleted cell-derived embryos. (springer.com)
  • Taken together, manipulating mtDNA in donor cells and/or oocytes prior to SCNT could enhance embryo production efficiency. (springer.com)
  • With her research, Platt challenged then current theories about germ layers, the types of cells in an early embryo that develop into adult cells. (asu.edu)
  • Meiosis, the process by which sexually-reproducing organisms generate gametes (sex cells), is an essential precondition for the normal formation of the embryo. (asu.edu)
  • Willadsen coated the embryo with an agar jelly made from algae to reduce the damage caused by entry of the glass needle into the cell membrane. (asu.edu)
  • Driesch showed that the cells of an early embryo, when separated, could each continue to develop into normal larval forms. (asu.edu)
  • Conrad Hal Waddington's "Experiments on Embryonic Induction III," published in 1934 in the Journal of Experimental Biology, describes the discovery that the primitive streak induces the mammalian embryo. (asu.edu)
  • She produced chimeric mice (which she at first termed 'allophenic') by inclusion of two genetically different cells in the early mouse embryo, thereby revealing the clonal organization. (casinapioiv.va)
  • found that mouse teratocarcinoma stem cells developed normally in a normal embryo environment. (casinapioiv.va)
  • embryos
  • Furthermore, we have reviewed how mitochondrial supplementation in oocytes can have beneficial effects for SCNT embryos by increasing mtDNA copy number and the levels of expression of genes involved in energy production and decreasing the levels of expression of genes involved in embryonic cell death. (springer.com)
  • Platt followed the paths of cells in developing mudpuppy embryos to see how embryonic cells migrated during the formation of the head. (asu.edu)
  • Scientists have applied somatic cell nuclear transfer to clone human and mammalian embryos as a means to produce stem cells for laboratory and medical use. (asu.edu)
  • From this experiment, Willadsen made viable mammalian embryos using his modified techniques, but they didn't grow into adult organisms. (asu.edu)
  • NSCs
  • Therefore, these endogenous NSCs in the adult brain have been proposed as a potential source of neurons for neural tissue repair after various brain insults, such as ischemic stroke or traumatic brain injury (TBI) ( Kernie and Parent, 2010 ). (biologists.org)
  • Biology
  • This resulted in large tumor-like masses of cells that retained many markers of follicle stem cells, says Fuchs, who is Rebecca C. Lancefield Professor and head of the Laboratory of Mammalian Cell Biology and Development at Rockefeller and an investigator at the Howard Hughes Medical Institute. (webwire.com)
  • A strategy to counter multiple pathways of biochemical dysfunction linked to the development of vascular complications emerged from recent advances in understanding of the cell biology of diabetic complications: high-dose therapy with thiamine and related derivatives such as benfotiamine. (springer.com)
  • The Cell in Development and Inheritance, by Edmund Beecher Wilson, provided a textbook introduction to cell biology for generations of biologists in the twentieth century. (asu.edu)
  • 7 Division of Cardiovascular Diseases and 8 Department of Cell and Developmental Biology, University of Pennsylvania, Philadelphia, Pennsylvania, USA. (jci.org)
  • Although there are many unanswered questions, the study of autophagy and stem cell biology can help us to progress in life sciences. (springer.com)
  • regulates
  • However, research uncovering how a signaling pathway regulates stem cell behavior reveals that stem cells, once activated, enter a window of time during which they respond to their environment and retain their ability to alter their developmental path. (webwire.com)
  • The dynamic interaction of AMBRA1 with the dynein motor complex regulates mammalian autophagy. (springer.com)
  • Mammalian Atg18 (WIPI2) localizes to omegasome-anchored phagophores and positively regulates LC3 lipidation. (springer.com)
  • mice
  • In 2006, Kazutoshi Takahashi and Shinya Yamanaka reprogrammed mice fibroblast cells, which can produce only other fibroblast cells, to become pluripotent stem cells, which have the capacity to produce many different types of cells. (asu.edu)
  • Adult mice (2-4 months old, weighing ≥30 gm) were used for all of the experiments. (jneurosci.org)
  • The tumors seen in Kobielak s mice overexpress the sonic hedgehog signaling pathway, which is implicated in basal cell carcinomas. (webwire.com)
  • 1996
  • Campbell and his team also cloned a sheep from adult cells in 1996, which they named Dolly. (asu.edu)
  • In 1996, Keith Campbell, Jim McWhir, William Ritchie, and Ian Wilmut at the Roslin Institute in Edinburgh, UK, used nuclear transfer techniques to clone a sheep that was born and gre into an adult. (asu.edu)
  • They reported their results in the article 'Sheep Cloned by Nuclear Transfer from a Cultured Cell Line' in March 1996. (asu.edu)
  • gene
  • When the researchers inactivated the BMP pathway using conditional inducible gene targeting in a mouse, the bulge expanded as the stem cells proliferated, but these cells couldn t differentiate into hair follicles because the key signal they needed to progress to the next step of the cycle was lost. (webwire.com)
  • The proposal would provide for $500 million in bond funding that would be earmarked for capital projects, including a stem cell bank and gene research center at the University of Massachusetts Medical School. (rutgers.edu)
  • nuclei
  • The mudpuppy is an aquatic salamander commonly used by embryologists because its large embryonic cells and nuclei are easy to see. (asu.edu)
  • Somatic cells donate their nuclei, which scientists transplant into eggs after removing their nucleuses (enucleated eggs). (asu.edu)
  • Gurdon's first experiment in 1958 showed that the nuclei of Xenopus cells maintained their ability to direct normal development when transplanted. (asu.edu)
  • In 1962 researcher John Bertrand Gurdon at the University of Oxford in Oxford, England, conducted a series of experiments on the developmental capacity of nuclei taken from intestinal epithelium cells of feeding tadpoles. (asu.edu)
  • The experiments indicated how to reprogram nuclei from differentiated cells to produce live offspring, and that a single population of differentiated cells could produce multiple offspring. (asu.edu)
  • Stem Cell
  • The California Stem Cell Research and Cures Act, also called Proposition 71, was a ballot initiative proposed by California voters in 2004 to allocate three billion dollars of state funds for stem cell research over ten years. (asu.edu)
  • Endorsed by California scientists and patient-advocates, Prop 71 passed on 2 November 2004, amending the state constitution to make stem cell research a constitutional right. (asu.edu)
  • In the April 5 Cell Stem Cell, Maura Boldrini and colleagues at Columbia University, New York, report that adult neurogenesis not only exists, but remains steady into old age. (alzforum.org)
  • Cell Stem Cell, 2018. (alzforum.org)
  • Disturbing a stem cell from its initial quiescent state was once thought to taint its gold-standard properties. (webwire.com)
  • But until now, researchers didn t know how it controls stem cell behavior during the hair cycle. (webwire.com)
  • President George W. Bush vetoed a bill that would have lifted restrictions on stem cell research in June, but Massachusetts Governor Deval L. Patrick '78 has decided that his state will not wait for the federal government's consent before pressing on with the issue. (rutgers.edu)
  • An amendment to the bill would expand federal funding for embryonic stem cell research by changing a critical date relating to the eligibility of stem cell lines. (rutgers.edu)
  • The amendment would fundamentally change current federal embryonic stem cell policy by permitting federally-funded research on stem cell lines that were derived before June 15 of this year, as opposed to the current marker of August 9, 2001. (rutgers.edu)
  • The committee noted that this moving of the date forward by almost six years will advance progress toward treatments and cures by significantly expanding the number of stem cell lines eligible for federally funded research. (rutgers.edu)
  • Therefore, exploring the mechanism of autophagy in maintaining stem cell homeostasis is crucial. (springer.com)
  • humans
  • Tracking neurogenesis in humans has been trickier, but a seminal study two decades ago set the stage: Five terminal cancer patients received an injection of bromodeoxyuridine (BrdU), a dye that incorporates into DNA during cell division. (alzforum.org)
  • Mitochondrial diseases in humans result when the small organelles called mitochondria, which exist in all human cells, fail to function normally. (asu.edu)
  • progeny
  • This was a surprise for us because many people thought that quiescence was a fundamental feature of follicle stem cells, and that once activated, the proliferating progeny enter a lineage and become irreversibly committed, says Fuchs. (webwire.com)
  • somatic cell
  • In somatic cell nuclear transfer (SCNT), there is an issue with the mixing of two populations of mtDNA, namely from the donor cell and recipient oocyte. (springer.com)
  • Bowles EJ, Tecirlioglu RT, French AJ, Holland MK, St. John JC (2008) Mitochondrial DNA transmission and transcription after somatic cell fusion to one or more cytoplasts. (springer.com)
  • Dolly was the first mammal cloned from specialized adult (somatic) cells with the technique of somatic cell nuclear transfer (SCNT). (asu.edu)
  • laboratory
  • In 1995, Campbell and his scientific team used cells grown and differentiated in a laboratory to clone sheep for the first time. (asu.edu)