For example, in gel permeation chromatography, special inert beads are used as the stationary phase, and the mobile phase carries the analyte past those beads. (coleparmer.com)
Second, qualitative and quantitative characterization of the extracted HPMs was performed using high performance gel permeation chromatography (HPGPC). (biomedcentral.com)
Mass spectrometry2
Analytical techniques must be used to isolate and characterize new compounds, and sensitive analytical tools such as mass spectrometry and high-resolution chromatography are required to identify the least-abundant molecules. (biomedcentral.com)
Gel electrophoresis and related techniques became the basis for a wide range of biochemical methods, such as protein fingerprinting, Southern blot, other blotting procedures, DNA sequencing, and many more. (wikipedia.org)
Starch gel (and later polyacrylamide and other gels) enabled the efficient separation of proteins, making it possible with relatively simple technology to analyze complex protein mixtures and identify minute differences in related proteins. (wikipedia.org)
Protein and peptide chromatography prior to MS analysis can partly address this issue. (hindawi.com)
Cell-free system -- A mixture of cytoplasmic and/or nuclear components from cells used for in vitro protein synthesis, transcription, DNA replication, or other purposes. (nih.gov)
For native protein gels, tris-glycine buffer is the first choice. (itwreagents.com)
Polyacrylamide Gel Electrophoresis is typically used for separation of proteins, but also DNA (especially to visualize differences between small fragments) can be separated by PAGE. (itwreagents.com)
The separation capacity of a polyacrylamide gel is determined by the mixing ratio of acrylamide to bisacrylamide. (itwreagents.com)
Ionic5
In the first dimension, ion-exchange chromatography (IEC) separates peptides based on ionic interactions with the solid phase and an increasing salt buffer. (hindawi.com)
Gel electrophoresis -- The separation of the molecules of various ionic compounds dissolved in solution based on the compounds' different rates of movement through an electric field applied to the solution. (nih.gov)
Gel electrophoresis uses a gel in the form of tubes or a thin slab as a non-reacting support medium, which holds the molecules of the ionic compounds as they move in the conducting medium, in order to prevent diffusion of the compounds. (nih.gov)
Major and exciting advance can be found in both characterization of a water molecule confined in gel matrix and finding of novel critical phenomena of the ionic gel. (go.jp)
In the present review, comprehensive characteristics of free water, bound water and unfrozen water in gel, freezing-thawing mechanism of gel, and effect of molecular void space of gel are referred in the first half, and the reversible, discontinuous volume phase transitions of the ionic gels are outlined in the latter half. (go.jp)
Purification2
Chromatography can be used in a myriad of applications from analyzing miniscule samples to production-scale use as a purification step. (coleparmer.com)
Chromatography -- Any of several techniques for the separation or purification of complex mixtures that rely on the differential affinities, or the attractions or forces between particles that cause them to combine, of substances for a gas or liquid mobile medium (such as gelatin) and for a stationary adsorbing medium (such as paper) through which they pass. (nih.gov)
Separation2
At its core, chromatography is the interaction between a mobile phase (which carries the mixture being separated) and a stationary phase (which performs the separation). (coleparmer.com)
Today, most applications of chromatography are generally less colorful but the underlying principle of separation remains unchanged. (coleparmer.com)
Proteins1
All the venomous species have a venom gland that synthesizes, stores and secretes a complex mixture of biological molecules including proteins. (nature.com)
Apparatus2
The apparatus designed by Arne Tiselius enabled a range of new applications of electrophoresis in analyzing chemical mixtures. (wikipedia.org)
In this experiment he used the classic two-chambered apparatus configuration that he originally tested in 1953 (Miller 1953, 1955). (pafr-signal.com)
Liquid4
Subchronic dermal toxicity studies of Alcide Allay® gel and liquid in rabbits. (cdc.gov)
was effectively achieved by reversed-phase high-performance liquid chromatography (HPLC). (go.jp)
Molecules1
By the 1960s, increasingly sophisticated gel electrophoresismethods made it possible to separate biological molecules based on minute physical and chemical differences, helping to drive the rise of molecular biology. (wikipedia.org)
Electrophoresis4
The method spread slowly until the advent of effective zone electrophoresismethods in the 1940s and 1950s, which used filter paper or gels as supporting media. (wikipedia.org)
Zone electrophoresis found widespread application in biochemistry after Oliver Smithies introduced starch gel as an electrophoretic substrate in 1955. (wikipedia.org)
Most chromatographymethods have an inert mobile phase that carries the analyte through a long stationary phase housed inside a column. (coleparmer.com)
Compounds2
A variety of chromatography equipment is also available that can provide an accurate identification of known compounds or in-depth data about unknown compounds. (coleparmer.com)
Although the content of different SCC mixtures may vary, two compounds commonly found in commercial SCC are trisodium copper chlorin e 6 and disodium copper chlorin e 4 ( Figure 2 ). (oregonstate.edu)
Chlorophyll2
In this example, chlorophyll is the analyte mixture or sample, the sheet is the stationary phase, and the solvent is the mobile phase. (coleparmer.com)
Sodium copper chlorophyllin (SCC) is a semi-synthetic mixture of water-soluble sodium copper salts derived from chlorophyll. (oregonstate.edu)
Complex1
This allows for the analysis of unique parts within a complex mixture, such as identifying sugars within a given food. (coleparmer.com)
Molecular1
Pre-Photosynthesis Days (1955): Govindjee's early fascination with paper chromatography and virus infection: first paper published in Nature Major discoveries and contributions of Govindjee in understanding molecular mechanisms of Photosynthesis. (liverxreceptor.com)
Chemical1
40861669 tender for supply of chemical items at rims, ranchi 1 list of chemicall items : 2 20*c mini cooler ( it should be made up of polycarbonate and non toxic gel, must contain atleast 12 places for 1.5ml tube. (jharkhandtenders.net)
Analysis1
They help you create a consistent mixture by thoroughly combining different samples, ensuring you get reliable results from your analysis. (coleparmer.ca)
Technique2
Chromatography is a technique for separating mixtures into their constituent parts. (coleparmer.com)
Regardless of how it is used, the overall effectiveness of chromatography largely depends on choosing the right technique and phases to use within that technique. (coleparmer.com)
Paper1
Working on this project in Ranjan's laboratory, he published his first paper (Laloraya and Govindjee 1955) in Nature. (liverxreceptor.com)
Special1
SHIPS TELBON" shows these special hobbies, from Silica Gel Springs, hollow data and wheels from this book. (camfrogift.com)
Separate1
At its inception, chromatography was used to separate plant pigments into their contributing chemicals. (coleparmer.com)
Components1
The lower the ratio of these two components in the mixture, the higher the degree of crosslinking. (itwreagents.com)
Means1
This means that a 6 % gel prepared from a stock solution of a mixing ratio of 29 : 1, has a higher degree of crosslinking than a 6 % gel prepared from a stock solution of a mixing ratio of 37.5 : 1. (itwreagents.com)
Sample1
It's the process of breaking down your sample to create a uniform mixture. (coleparmer.ca)
Solution3
To the resulting solution was added 5.7 g of NaBH 4 in three portions and the resulting mixture was stirred at room temperature for 8 hours. (ambeed.com)
To prepare 100 ml of a 6 % acrylamide gel, 20 ml of the 30 % acrylamide stock solution is added into the gel-mixture. (itwreagents.com)
To initiate the polymerization, 100 µl of 10 % APS and 5-10 µl TEMED per 10 ml gel solution are added. (itwreagents.com)