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  • yeast
  • Here we show that deleting the yeast 14-3-3 protein, Bmh1, extends chronological life span (CLS) by activating the stress response. (genetics.org)
  • Our data indicate that that yeast cells can be in either of two states, expressing or not expressing genes required for high-affinity phosphate uptake and that 14-3-3 proteins are involved in the process(es) that establish the activation state of the PHO regulon. (biomedcentral.com)
  • In the fission yeast S. pombe, Mid1 is an important dimeric CAR scaffolding protein that connects the contractile apparatus to the plasma membrane at the right place and time during cytokinesis. (gvsu.edu)
  • Two members of the 14-3-3 protein family have been isolated in a yeast two-hybrid screen designed to identify proteins that interact with the human cdc25A and cdc25B phosphatases. (cshl.edu)
  • pathways
  • The 14-3-3 family of proteins plays a key regulatory role in sigl transduction, checkpoint control, apoptotic and nutrient-sensing pathways. (acris-antibodies.com)
  • A gene on chromosome 8q23.1 that encodes an adapter protein which regulates a broad spectrum of general and specialised signalling pathways by binding to a large number of partners, usually by recognising a phosphoserine or phosphothreonine motif. (thefreedictionary.com)
  • Calorie restriction (CR) and conserved nutrient-sensing target of rapamycin (TOR) and protein kinase A (PKA) pathways have also been suggested to extend life span by increasing stress response, which protects cells from age-dependent accumulation of oxidative damages. (genetics.org)
  • Antibodies
  • In order to further improve the detection sensitivity of 14-3-3, a sandwich time-resolved fluoroimmunoassay (TRFIA) was developed using a pair of monoclonal antibodies, and it could be shown that TRFIA has a higher sensitivity in detecting 14-3-3 antigen of S. japonicum than an enzyme-linked immunosorbent assay (ELISA). (biomedcentral.com)
  • First, immunoprecipitation experiments, using isoenzyme-specific monoclonal Lox antibodies, showed that 14-3-3 proteins co-precipitate with 13-Lox, but not with the 9-Lox from barley. (lancs.ac.uk)
  • sigma
  • Sequestration of Bax by 14-3-3 sigma inhibits apoptosis whereas sequestration of 14-3-3 sigma by CARP-1 induces it. (acris-antibodies.com)
  • Mitosis is regulated by 14-3-3 sigma through binding keratin filaments and sequestering cyclin B1 and cdc2. (acris-antibodies.com)
  • p73-dependent induction of 14-3-3 sigma regulates chemo sensitivity in breast cancers with p53 mutations. (acris-antibodies.com)
  • In reaction to DNA damage and along with RACK1, 14-3-3 sigma targets, DeltaNp63alpha for proteasome degradation. (acris-antibodies.com)
  • MBS7246827 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the 14-3-3 protein sigma (SFN) ELISA Kit target analytes in biological samples. (mybiosource.com)
  • Barley
  • Barley 14-3-3 transcripts accumulate in the epidermis upon inoculation with the powdery mildew fungus. (deepdyve.com)
  • bound
  • When bound to KRT17, stratifin regulates protein synthesis and epithelial cell growth by stimulating the AKT/MTOR pathway. (thefreedictionary.com)
  • During the first hour of salt stress, the rate of proton extrusion from the excised roots increased in parallel with the ATP hydrolase activity and the amount of 14-3-3 proteins bound to H+-ATPase in isolated plasma membranes. (deepdyve.com)
  • In this work, we found that the apo-14-3-3σ is in an open state compared with the phosphopeptide bound 14-3-3σ complex which is in a more closed state based on our 80 ns molecular dynamics (MD) simulations. (mdpi.com)
  • However, large differences are found in helices E and F. The hydrophobic contacts and hydrogen bonds between helices E and G in apo-14-3-3σ are different from those in the bound 14-3-3σ complex. (mdpi.com)
  • sequence
  • View conserved domains detected in this protein sequence using CD-search. (nih.gov)
  • Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed. (uniprot.org)
  • p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence. (uniprot.org)
  • p>This subsection of the 'Names and taxonomy' section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. (uniprot.org)
  • Sequence analysis is suggestive of common biochemical functions for these tomato 14-3-3 proteins, but their genes showed different expression patterns in leaves after challenges. (lancs.ac.uk)
  • molecular
  • Molecular binding mode involving 14-3-3 will be elucidated by NMR. (findaphd.com)
  • Importantly, these activities are usually independent of substrate phosphorylation and therefore should be considered as distinct, 'moonlighting' functions of 14-3-3 proteins that mimic and complement the functions of dedicated molecular chaperones. (ovid.com)
  • Understanding the latter mode of 14-3-3 functioning is fundamental to defining the underlying molecular mechanisms for a range of human disorders. (ovid.com)
  • Hu G, Li H, Liu J-Y, Wang J. Insight into Conformational Change for 14-3-3σ Protein by Molecular Dynamics Simulation. (mdpi.com)
  • overexpression
  • p>Describes annotations that are concluded from looking at variations or changes in a gene product such as mutations or abnormal levels and includes techniques such as knockouts, overexpression, anti-sense experiments and use of specific protein inhibitors. (uniprot.org)
  • plasma membrane
  • Combined immunological and proteomic approaches revealed that phosphorylation at two C-terminal sites (Ser280, Ser283) of PLASMA MEMBRANE INTRINSIC PROTEIN 2;1 ( At PIP2;1), a major plasma membrane aquaporin in rosettes, shows circadian oscillations and is correlated with K ros . (plantcell.org)
  • Overlay assays show a fungus-induced increase in binding of digoxygenin-labelled 14-3-3 protein to several proteins including a 100 kDa membrane protein, probably the plasma membrane H+-ATPase. (deepdyve.com)
  • We propose that 14-3-3 proteins are involved in an epidermis-specific response to the powdery mildew fungus, possibly via an activation of the plasma membrane H+-ATPase. (deepdyve.com)
  • biochemical
  • However, diagnostic biochemical parameters including 14-3-3 protein, S100, neuron-specific enorase in cerebrospinal fluid (CSF) have been used as diagnostic markers, elevated titers of these markers can also be observed in CSF in other neurodegenerative diseases. (springer.com)
  • regulators
  • Furthermore, although tremendous progress has been made in recent years in identifying proteins that participate in checkpoint control, it is unclear how these proteins interface with core cell cycle regulators to inhibit cell cycle transitions ( 1 ). (sciencemag.org)
  • elisa
  • Within the first 21 days post-infection in rabbits, the positive rates of the 14-3-3-TRFIA were distinctly higher compared to ELISA. (biomedcentral.com)
  • Small volumes of 14-3-3 Pro elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. (mybiosource.com)
  • The ELISA Kit is designed to detect native, not recombinant, 14-3-3 Pro. (mybiosource.com)
  • localization
  • Grawe, Anissa P., "14-3-3 Protein Rad24 and its Effect on Mid1 Localization and Contractile Acto- Myosin Ring Assembly During Cytokinesis in S. pombe" (2018). (gvsu.edu)
  • expression
  • Following transient expression in planta, HopQ1 was shown to copurify with host 14-3-3 proteins. (plantphysiol.org)
  • Thus, the expression of a specific bacterial effector can either sustain disease in susceptible plants or render the pathogen avirulent in resistant plants that express the corresponding R protein. (plantphysiol.org)
  • Microscopic and flow cytometric analysis of cells with GFP-tagged SPL2 showed that disruption of BMH1 resulted in two populations of genetically identical cells, cells expressing the protein and the majority of cells with no detectible expression. (biomedcentral.com)
  • In these cells, expression of Cdc25C and Cdc25(S216A) is under the control of a hybrid protein consisting of a bacterial tetracycline repressor and VP16 activator protein ( 3 ). (sciencemag.org)
  • A ) Expression of Cdc25C and Cdc25(S216A) was induced by removal of tetracycline (Tet) from the media ( 3 ). (sciencemag.org)
  • assay
  • Therefore, we concluded that the assay of t-tau protein may be useful as 1st screening and the ratio of p-tau protein/t-tau protein would be useful as 2nd screening to discriminate CJD from other neurodegenerative diseases. (springer.com)
  • plant
  • HopQ1 (for Hrp outer protein Q), a type III effector secreted by Pseudomonas syringae pv phaseolicola , is widely conserved among diverse genera of plant bacteria. (plantphysiol.org)
  • In this study, the role of a 14-3-3 protein, GRF9, in plant response to water stress was investigated. (ovid.com)
  • identify proteins
  • To identify proteins that may contribute to this retention, [35S]Met-labeled Gen10 fusion proteins with the 3i loops of the alpha2AAR (Val217-Ala377), alpha2BAR (Lys210-Trp354), and alpha2CAR (Arg248-Val363) were used as ligands in gel overlay assays. (rti.org)
  • neurodegenerative disorders
  • Recent evidence that 14-3-3 proteins interfere with protein aggregate formation suggests they can also display a distinct, usually phosphorylation-independent, chaperone-like activity, which we review here as their 'moonlighting' function, which is of particular relevance in the context of neurodegenerative disorders. (ovid.com)
  • We analyzed CSF samples derived from 100 patients with various neurodegenerative disorders by Western blot of 14-3-3 protein, quantification of total tau (t-tau) protein, and phosphorylated tau (p-tau) protein. (springer.com)