1 overexpression. Medical search. Frequent questions

These results establish a novel RFC-based metabolic selection system for the efficient overexpression of recombinant proteins. (aspetjournals.org)
The IGF-1 gene was cloned into the lentiviral shuttle plasmid pCDH-cGFP and the recombinant lentiviral vector was transducted into myoblast C2C12 cell line. (cellmolbiol.org)
The results of our study suggests that constructed recombinant lentiviral vector can potentially be used for regulating the expression of IGF-1 in myoblast C2C12 cells. (cellmolbiol.org)
IGF-1 overexpression recombinant lentiviral gene therapy. (cellmolbiol.org)
The effects of the overexpression of recombinant uncoupling protein 2 on proliferation, migration and plasminogen activator inhibitor 1 expression in human vascular smooth muscle cells. (cims-ops.cz)

PGC-1α overexpression in hepatocytes lowered expression of genes critical to VLDL assembly and secretion (apolipoprotein B and microsomal triglyceride transfer protein). (eurekamag.com)
The results demonstrated that the levels of mRNA transcription and protein expression of the TJ‑associated proteins, claudin‑5, occludin and ZO‑1, were significantly reduced following TBI. (spandidos-publications.com)
Haskins J, Gu L, Wittchen ES, Hibbard J and Stevenson BR: ZO-3, a novel member of the MAGUK protein family found at the tight junction, interacts with ZO-1 and occludin. (spandidos-publications.com)
Moreover, CR-1 mRNA and protein levels were significantly higher in HCC tissues than adjacent normal liver tissues. (aging-us.com)
Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). (novusbio.com)
Protein arginine methyltransferase 1 may be involved in pregnane x receptor-activated overexpression of multidrug resistance 1 gene during acquired multidrug. (oncotarget.com)
In the present study, we aimed to investigate whether protein arginine methyl transferase 1(PRMT1) is involved in PXR - activated overexpression of MDR1 during acquired multidrug resistant. (oncotarget.com)
Furthermore, moderate projection neuron-specific overexpression of Ig-NRG1 allowed us to investigate the subcellular localization of the protein, which appeared to be associated with the potassium channel Kv2.1 in the somatodendritic compartment. (uni-goettingen.de)
In order to investigate the mechanism of APS regulating m6A modification level, the authors first examined whether APS treatment could affect the expression of the protein related to the m6A modification system in LPS-induced THP-1 macrophages. (ubigene.us)
As shown in Figure 1, the mRNA expression of WTAP was significantly reduced after APS treatment for 24 h, and the protein level of WTAP was also significantly reduced compared with other related proteins, indicating that APS can affect m6A modification level by regulating WTAP expression. (ubigene.us)
To verify whether APS affects the expression of IL-6 through WTAP, the authors detected the expression of IL-6 mRNA and protein after WTAP overexpression by qRT-PCR and WB, respectively. (ubigene.us)
The expression of Cripto-1 at mRNA level was detected by the reverse transcription-polymerase chain reaction (RT-PCR) and real time RT-PCR, and western blot was used to examine the protein expression. (biomedcentral.com)
The results of real-time RT-PCR and western blot showed that the expression level of Cripto-1 was markedly higher in NPC cell lines than that in the immortalized nasopharyngeal epithelial cell at both mRNA and protein levels. (biomedcentral.com)
Mutated HIF-1α protein half-life was similar to that of the wild-type. (tau.ac.il)
Tumor protein 53-induced nuclear protein 1 (TP53INP1) is a proapoptotic protein involved in cell stress response. (urotoday.com)
Elevated muscle PGC-1α completely preserves muscle mass by preventing the fall in pro-fusion protein expression, AMPK and catabolic system activation, suggesting that compounds inducing PGC-1α expression could be useful to treat and prevent muscle atrophy. (unipv.it)
Using semi-quantitative reverse-transcription polymerase chain reaction (RT-PCR) and western blots experiments, we compared changes in ETS-1 and ETS-2 expression, their protein levels, and the regulation of some of their target gene expressions at different stages of the ocular tumoral progression in the transgenic mouse model, Tyrp-1-TAg, with those in normal eyes from control mice of the same age. (molvis.org)
ETS-1 and ETS-2 mRNA and protein levels were much higher in the ocular tissues of Tyrp-1-TAg mice than in control ocular tissues from wild-type mice. (molvis.org)
Immunohistochemistry was used to assess ABCC3 protein overexpression. (lu.se)
ABCC3 (MRP3) protein overexpression was more common in tumors with gene amplification (P = 0.069). (lu.se)

In this study, we investigated the role of embryonic gene Cripto-1 (CR-1) in hepatocellular carcinoma (HCC) using hepatocyte-specific CR-1-overexpressing transgenic mice. (aging-us.com)
Pregnane x receptor (PXR) - activated overexpression of the multidrug resistance 1 (MDR1) gene is an important way for tumor cells to acquire drug resistance. (oncotarget.com)
Compared with human peripheral blood mononuclear cells (PBMCs), THP-1 is easier to culture and expand in the laboratory, and has a more stable gene background, which can be used in transfection or infection experiments, and is conducive to the reproduction of experimental results. (ubigene.us)
Hypoxia-inducible factor 1α (HIF-1α) gene polymorphisms have been investigated for a possible role in mediating genetic predisposition to cancer. (tau.ac.il)
Insulin-like growth factor gene (IGF-1) is one of the most important growth factors that plays a key role in the proliferation and differentiation of muscle cells. (cellmolbiol.org)
In our study, we constructed a lentiviral vector inducing an overexpression of IGF-1 in order to study the regulating mechanisms of this gene. (cellmolbiol.org)
The overexpression of IGF-1 was confirmed by RT-PCR and western blotting for IGF-1 receptor gene. (cellmolbiol.org)
Inhibition of miRNA-26a increases gene expression of SMAD-1 and SMAD-4, while overexpression inhibits SMAD-1. (wikipedia.org)
To accelerate their osteogenic differentiation, we overexpressed the osteogenic transcription factor Cbfa1 (Core binding factor alpha-1, Til-1) gene using an adenovirus vector in rat ASCs. (jarde.jp)
METHODS: Overexpression of human UCP-2 was performed in primary cultured human VSMCs (HVSMCs) via adenovirus-mediated gene transfer. (cims-ops.cz)
Its effects on ROS production, AP-1 activity, plasminogen activator inhibitor 1 (PAI-1) gene expression, and cellular proliferation and migration were measured in response to high glucose and angiotensin II (Ang II) concentrations, two major factors in the pathogenesis of atherosclerosis in patients with diabetes and hypertension. (cims-ops.cz)
Chromosomal rearrangements have been observed between this locus and at least two loci, the delta subunit of the T-cell antigen receptor gene and the LIM domain binding 1 gene. (vectorbiolabs.com)
Each of these layers is characterized by specific features in terms of gene expression, function and cellular mor-phology (1). (medicaljournals.se)

Cripto-1 plays an important oncogenic role during tumorigenesis and is overexpressed in a wide range of epithelial carcinomas, yet little is known about Cripto-1 in nasopharyngeal carcinoma (NPC). (biomedcentral.com)
Data of this study suggest that Cripto-1 overexpression is connected with the tumorigenesis and progression of NPC, lentivector-mediated RNAi might be feasible for the inhibition of the growth and invasion of NPC. (biomedcentral.com)
Given a p53-silencing background, MCT-1 malignantly transforms normal breast epithelial cells that are satisfactory for stimulating cell migration/ adhesion and tumorigenesis. (tmu.edu.tw)
Role of Caveolin-1 in carbon nanotube -induced stem-like cells and tumorigenesis. (cdc.gov)
Overexpression of Cav-1 further promoted the CSC induction, cell motility, and in vivo tumorigenesis. (cdc.gov)
Our findings provide novel evidence for the induction of CSC-like cells by chronic SWCNT exposure, which are likely a driving force of SWCNT tumorigenesis, and demonstrated a positive regulatory role of Cav-1. (cdc.gov)
We suggest that detection of CSC may provide a valuable tool for early detection of CNT carcinogenesis and that Cav-1 might serve as a biomarker or drug target for CNT tumorigenesis. (cdc.gov)

They also significantly stimulated ROS production, NAD(P)H oxidase activity, mitochondrial membrane potential, AP-1 activity, PAI-1 mRNA expression, and proliferation and migration of HVSMCs. (cims-ops.cz)

Inhibition of endogenous Cripto-1 by lentivirus-mediated RNAi silencing technique suppressed NPC cell growth and invasion in vitro . (biomedcentral.com)
The lentivirus-mediated overexpression of psoriasin, mimicking the psoriatic milieu, gave rise to an altered regulation of differentiation genes and an expression pattern reminiscent of that in psoriatic epidermis. (medicaljournals.se)

Overexpression of cyclin D1 and cyclin E in 1,2-dimethylhydrazine dihydrochloride-induced rat colon carcinogenesis. (ewha.ac.kr)
To investigate the expression pattern of G1 cyclins in 1,2- dimethyl-hydrazine dihydrochloride (DMH)-induced rat colon carcinogenesis, we studied the expression of cyclin D1 and cyclin E by quantitative reverse transcription-polymerase chain reaction (RT-PCR) analysis and immunohistochemistry (IHC). (ewha.ac.kr)
Our results indicate that the overexpression of cyclin D1 and cyclin E may play an important role during the multistage process of rat colon carcinogenesis, at a relatively early stage, and may disturb cell-cycle control in benign adenomas, and thereafter, participate in tumor progression. (ewha.ac.kr)
101. Pathologically confirmed MCL, with documentation of either overexpression of cyclin D1 or presence of t(11;14) 102. (who.int)

Adenoviral transduction of rats with PGC-1α resulted in a liver-specific increase in PGC-1α expression and produced an in vivo liver phenotype of increased FAO via increased mitochondrial function that also resulted in reduced hepatic TAG storage and decreased plasma TAG levels. (eurekamag.com)
To understand the association between netrin‑1 and the TJ‑associated proteins, the expression levels of proteins involved in maintaining the integrity of the BBB, including netrin‑1, claudin‑5, occludin and zonula occluden (ZO)‑1, were investigated in the present study using quantitative polymerase chain reaction, western blot analysis and immunofluorescence. (spandidos-publications.com)
The aim of the present study was to determine the changes in BBB permeability and whether pZsGreen1‑N1 mediated overexpression of netrin‑1 increased the expression of the TJ‑associated proteins following traumatic brain injury (TBI). (spandidos-publications.com)
The present study also demonstrated that netrin‑1 significantly increased the downregulation of claudin‑5, occludin and ZO‑1 expression levels induced by TBI, which provided a basis for further investigation on the role of netrin‑1 in the integrity of TJs and proper functioning of the BBB. (spandidos-publications.com)
In this study, HIV-1 Subtype C tat and nef genes were cloned into an expression plasmid and their expression investigated in Salmonella. (ajol.info)
Very high-level expression of the two HIV-1 antigens was demonstrated in the recombinantSalmonella. (ajol.info)
The expression of truncated 1.7-kb CR-1 transcript (SF-CR-1) was significantly higher than the full-length 2.0-kb CR-1 transcript (FL-CR-1) in a majority of HCC tissues and cell lines. (aging-us.com)
Hepatocyte-specific over-expression of CR-1 in transgenic mice enhanced hepatocyte proliferation after 2/3 partial hepatectomy (2/3 PHx). (aging-us.com)
CR-1 over-expression significantly increased in vivo xenograft tumor growth of HCC cells in nude mice and in vitro HCC cell proliferation, migration, and invasion. (aging-us.com)
In the search for signals that cause breast cancer, many lines of investigation have linked cancer with elevated expression or altered function of receptor tyrosine kinases (1) . (aacrjournals.org)
AMI-1 significantly suppressed the expression of MDR1 in MCF7/adr cells and increased cells sensitivity of MCF7/adr to adriamycin. (oncotarget.com)
By constructing a stable expression THP-1 c ell line, it can be used to study the mechanism of immunity and inflammation. (ubigene.us)
WTAP overexpression improved the low expression of IL-6 in APS-treated THP-1 macrophages (Figure 2). (ubigene.us)
The authors hypothesized that WTAP regulated IL-6 through the NF-KB pathway, and detected the expression level of p65 in APS-treated THP-1 macrophages by WB. (ubigene.us)
WTAP overexpression did not affect the overall expression level of p65 in THP-1 macrophages. (ubigene.us)
Cripto-1 expression and its clinical characteristics were investigated by performing immunohistochemical analysis on a total of 37 NPC clinical tissue samples. (biomedcentral.com)
Lentiviral vectors were constructed to get an efficient expression of anti-Cripto-1 siRNA in CNE-2 and C666-1 cells, with invalid RNAi sequence as control. (biomedcentral.com)
Furthermore, Cripto-1 expression is significantly increased in premalignant lesions, such as colon adenomas, intestinal metaplasia of the gastric mucosa and ductal carcinoma in situ (DCIS) of the breast[ 7 ]. (biomedcentral.com)
Our results indicate that synergistic promotions of chromosomal imbalances and oncogenic potency as a result of MCT-1 expression and p53 loss play important roles in tumor development. (tmu.edu.tw)
Therefore, we studied the effects of C1772T polymorphism on HIF-1α expression. (tau.ac.il)
HIF-1αmRNA expression levels were significantly higher in peripheral blood leukocytes of prostate cancer patients with the TT genotype compared with the CC genotype. (tau.ac.il)
Expression of C1772T HIF-1α in HIF-1α knockout cancer cells showed higher expression levels and stabilization of HIF-1α mRNA compared with the wild-type. (tau.ac.il)
MicroRNA-26a targets the expression of SMAD-1 and SMAD-4, members of the TGF-βsuperfamily signaling cascade. (wikipedia.org)
The downregulation of psoriasin expression by small interfering RNA revealed that psoriasin mediates the expression of involucrin, desmoglein 1, transglutaminase 1 and CD24 in normal differentiation. (medicaljournals.se)

Compared to wild-type, overexpression (OE) lines had significantly higher shoot (2.6-3.5-fold) and root (7-10-fold) biomass on AsIII containing media and accumulated 40-60% less arsenic in root and shoot tissues. (authorea.com)
Clinical validations confirm that MCT-1 mRNA levels are differentially enriched in comparison between human lung cancer and nontumorigenic tissues. (tmu.edu.tw)
Whereas there is an overexpression of TP53INP1 in numerous tissues submitted to stress agents, TP53INP1 is down-expressed in stomach, pancreatic, and inflammation-mediated colic carcinomas. (urotoday.com)
Using intravital imaging and immunohistochemistry , we demonstrated that pressure promotes tumour growth in subcutaneous tumourigenesis nude mice , and overexpression of miRNA -5703 significantly downregulated Ki67 and upregulated NM23 in tumour tissues of mice , implying the blockage of tumour growth and metastasis . (bvsalud.org)

Meanwhile, the overexpression of miR-26a in the A549 human lung cancer cell line dramatically inhibits cell proliferation, blocks G1/S phase transition, induces apoptosis, and inhibits cell metastasis and invasion in vitro. (wikipedia.org)
Overexpression of pressure-responsive miRNA-5703 inhibits pressure-induced growth and metastasis of liver cancer. (bvsalud.org)

Here we report that simultaneous overexpression of two transcription factors (TFs) can direct the differentiation of human iPSCs to granulosa-like cells. (biorxiv.org)
Overexpression of miRNA-26a blunts differentiation. (wikipedia.org)

However, JAM-C overexpression on endothelial cells did not accelerate diabetes in the RIP-LCMV model. (crcm-marseille.fr)
The effect of endothelial cell overexpression of plasminogen activator inhibitor-1 on smooth muscle cell migration. (ouhsc.edu)

Elongation factor 1 gamma (EF1γ) has been isolated from a pancreatic cancer cell line, while the overexpression of EF1γ has also been observed in colorectal carcinomas and adenomas. (elsevierpure.com)
An overexpression was observed in 22 (73%) of 30 gastric carcinomas, relative to the corresponding normal tissue. (elsevierpure.com)
In medullary thyroid carcinomas, TP53INP1 overexpression correlates with poor prognosis. (urotoday.com)

However, histological signs of precancerous lesions, hepatocyte dysplasia or HCC formation were not observed in the livers of 3-, 6- or 8-month-old hepatocyte-specific CR-1-overexpressing transgenic mice. (aging-us.com)
These findings demonstrate that liver-specific CR-1 overexpression in transgenic mice deregulates signaling pathways and genes associated with HCC. (aging-us.com)
Ultrastructural analysis revealed that overexpression of CD97 strengthened lateral cell-cell contacts between enterocytes, which, in contrast, were weakened in CD97 knockout (Ko) mice. (nih.gov)
Here we have shown that the dystrophic phenotype observed in δ-sarcoglycan-null (Sgcd-/-) mice and dystrophin mutant mdx mice is dramatically improved by skeletal muscle-specific overexpression of sarcoplasmic reticulum Ca2+ ATPase 1 (SERCA1). (jci.org)
Blockade but not overexpression of the junctional adhesion molecule C influences virus-induced type 1 diabetes in mice. (crcm-marseille.fr)
In vivo , the average weight ( p = 0.026) and volume ( p = 0.044) of tumor in CNE-2/GFP + /Cripto-1 - xenotransplant mice group were significantly lower than those in the control group. (biomedcentral.com)
Human Cripto-1, also known as teratocarcinoma-derived growth factor-1 (TDGF-1), is a member of the Epidermal growth factor-cripto FRL1 cryptic (EGF-CFC) family (Cripto in humans, FRL1 in Xenopus, and Cryptic in mice), which is indispensable for early embryonic development[ 4 ]. (biomedcentral.com)
We have studied the transgenic mouse strain, Tyrp-1-TAg, to try to gain insight into possible molecular mechanisms common to pigmented ocular neoplasms occurring spontaneously in the eyes of these mice and human choroidal melanoma. (molvis.org)
This is the first study describing the production and distribution of ETS-1 and ETS-2 mRNAs and proteins using in situ hybridization and immunohistochemistry in murine ocular tissue sections of normal control eyes and tumoral eyes from mice of the same age. (molvis.org)
We also demonstrated upregulation of ETS-1 and ETS-2 target expressions in Tyrp-1-TAg mice when comparing with the same target expressions in control mice. (molvis.org)
In mice, overexpression of human SORLA in murine adipose tissue blocked hydrolysis of triacylglycerides and caused excessive adiposity. (medscape.com)

In vitro, CT-1 promotes the survival and induces hypertrophy of neonatal cardiomyocytes. (utmb.edu)

This study tested the hypothesis that double overexpression of miR-19a & miR-20a (dOex-mIRs) in human induced pluripotent stem cell (iPS)-derived mesenchymal stem cells (MSCs) effectively preserved left-ventricular-ejection-fraction (LVEF) in dilated cardiomyopathy (DCM) (i.e., induced by doxorubicin) rat. (researchsquare.com)
Assuredly, idiopathic dilated cardiomyopathy (DCM), a primary non-ischemic cardiac muscle disease, is clearly recognized as the consequence of systolic dysfunction and dilatation of chamber size of the left or both of ventricles [1, 2]. (researchsquare.com)

Recent studies have identified overexpression of HER2 or epidermal growth factor receptor in some tumors and used this knowledge to develop successful approaches for therapeutic targeting of cancer cells (2) . (aacrjournals.org)
However, overexpression of HER2 and epidermal growth factor receptor is limited to a subset of tumors, which creates a need to identify other tyrosine kinases that are responsible for cancer progression and pathogenesis. (aacrjournals.org)
The role of two members of the ETS (E26 avian leukemia oncogene) family of transcription factors, ETS-1 and ETS-2, has been investigated in many cancers but has not yet been studied in ocular tumors. (molvis.org)

Genetic and metabolic analyses of Escherichia coli strains showed that overexpression of PanB leads to accumulation of the likely folate cleavage product 6-hydroxymethylpterin and other pterins in cells and medium, and-unexpectedly-to a 46% increase in total folate content. (ebi.ac.uk)

As of January 1, 2022, Oncotarget has shifted to a continuous publishing model. (oncotarget.com)
J Cancer;13(1): 325-342, 2022. (bvsalud.org)
Sigma-Aldrich n.d. https://www.sigmaaldrich.com/US/en/tech-docs/paper/1413766 (accessed April 1, 2022). (bvsalud.org)

We also show that EphA2 overexpression is sufficient to confer malignant transformation and tumorigenic potential on nontransformed (MCF-10A) mammary epithelial cells. (aacrjournals.org)

Interestingly, while KLF6-SV1 overexpression increased metastasis, it did not affect localized tumor growth. (jci.org)
In addition, Cripto-1 overexpression was significantly associated with N classification ( p = 0.034), distant metastasis ( p = 0.036), and clinical stage ( p = 0.007). (biomedcentral.com)

Neuregulin 1 (NRG1) is the best characterized member of a family of epidermal growth factor (EGF)-like domain containing proteins that serve as ligands for tyrosine kinase receptors of the ErbB family. (uni-goettingen.de)

MCT-1 oncoprotein accelerates p53 degradation by means of the ubiquitin-dependent proteolysis. (tmu.edu.tw)

Thus, in contrast to projection neuron-specific loss- or (moderate) overexpression of NRG1, these findings suggest that NRG1-mediated autocrine signaling in Parv+ interneurons serves an essential role in the regulation of inhibitory circuit functions. (uni-goettingen.de)
Moderate overexpression in clonally selected cell lines did not have a detectible effect on actin filament content or organization. (rupress.org)
The 2019 ASGE guideline found insufficient evidence on the effectiveness of screening for Barrett esophagus, but suggested that if screening endoscopy is performed, it should be done in an at-risk population, defined as individuals with a family history of esophageal adenocarcinoma or Barrett esophagus (high risk) or patients with GERD plus at least 1 other risk factor (moderate risk). (medscape.com)

The bone formation was markedly increased by the overexpression, compared to the control which partially dedifferentiated into adipocytes by 8 weeks after implantation. (jarde.jp)

Type 1 diabetes (T1D) results from the autoimmune destruction of insulin-producing beta-cells in the pancreas. (crcm-marseille.fr)

We examined whether peroxisome proliferator-activated receptor-γ coactivator-1α (PGC-1α) overexpression and a subsequent increase in mitochondrial content and function in rat primary hepatocytes (in vitro) and Sprague-Dawley rats (in vivo) would comprehensively alter mitochondrial lipid metabolism, including complete (CO(2)) and incomplete (acid-soluble metabolites) fatty acid oxidation (FAO), tricarboxylic acid cycle flux, and triacylglycerol (TAG) storage and export. (eurekamag.com)
PGC-1α overexpression in primary hepatocytes produced an increase in markers of mitochondrial content and function (citrate synthase, mitochondrial DNA, and electron transport system complex proteins) and an increase in FAO, which was accompanied by reduced TAG storage and TAG secretion compared with control. (eurekamag.com)
In conclusion, overexpression of hepatic PGC-1α and subsequent increases in FAO through elevated mitochondrial content and/or function result in reduced TAG storage and secretion in the in vitro and in vivo milieu. (eurekamag.com)
Mitochondrial fusion and fission have emerged as important processes that govern mitochondrial function and PGC-1α may regulate fusion/fission events. (unipv.it)

Further, SERCA1 overexpression almost completely rescued the dystrophic phenotype in a mouse model of MD driven solely by Ca2+ influx. (jci.org)
The motile phenotype was similar to that observed with gelsolin overexpression, which in addition to capping, also severs and nucleates actin filaments. (rupress.org)

abstract = "Cardiotrophin-1 (CT-1), a cytokine with structural similarities to interleukin-6, has been shown to signal through gp130-dependent pathways. (utmb.edu)

Also, the PGC-1α-overexpressing hepatocytes were protected from excess TAG accumulation following overnight lipid treatment. (eurekamag.com)
Overexpression of gamma-Glutamyl Cyclotransferases 2;1 (CsGGCT2;1) Reduces Arsenic Toxicity and Accumulation in Camelina sativa (L. (authorea.com)
Here we characterized the GGCT2;1 homolog from Camelina sativa for its role in arsenic tolerance and accumulation. (authorea.com)
Overall, overexpression of GGCT2;1 and it homologs in Camelina and other crops will enable their cultivation on contaminated marginal lands to reduce As accumulation, addressing food safety issues as well as future food and biofuel needs. (authorea.com)

Our present data show that induction of MCT-1 increases chromosomal translocations and deregulated G 2 -M checkpoint in response to chemotherapeutic genotoxin. (tmu.edu.tw)
Remarkably, increases in chromosome copy number, multinucleation, and cytokinesis failure are also promoted while MCT-1 is induced in p53-deficient cells. (tmu.edu.tw)

These CSC-like cells, which express a high level of Cav-1, displayed increased cell migration and invasion, compared with their non-CSC counterpart. (cdc.gov)

CDK4 overexpression is a predictive biomarker for resistance to conventional chemotherapy in patients with osteosarcoma. (cdc.gov)

There was a small but nonsignificant increase in 5-oxoproline (5-OP) in OE lines and the upregulation of Oxoprolinase 1 ( OXP1 ) suggested accelerated conversion of 5-OP to glutamate, which is then utilized for GSH resynthesis for maintaining homeostasis. (authorea.com)

The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. (novusbio.com)
Arginine methyltransferase inhibitor 1 (AMI-1) was used to pharmacologically block PRMT1 in resistant breast cancer cells (MCF7/adr). (oncotarget.com)

For this purpose, a conditional transgenic mouse line was produced that permits stage- and cell type-specific overexpression of the Ig-NRG1 isoform under control of the β-actin promoter after Cre-mediated removal of a "floxed" STOP-cassette. (uni-goettingen.de)
Finally, in a pilot project a new mouse line for the conditional overexpression of NRG2, a closely related ErbB4 ligand in the brain, has been generated by homologous recombination into the Rosa26 locus of embryonic stem cells. (uni-goettingen.de)

CD97 overexpression upregulated membrane-bound but not cytoplasmic or nuclear beta-catenin and reduced phospho-beta-catenin, labeled for degradation. (nih.gov)
In order to better study the nuclear and cytoplasmic distribution of p65, nuclear and cytoplasmic proteins were extracted from THP-1 macrophages. (ubigene.us)

To date, various genome-scale loss-of-function screening methods have been developed, including approaches employing RNA interference 1 , 2 and the RNA-guided endonuclease Cas9 from the microbial CRISPR (clustered regularly interspaced short palindromic repeat) adaptive immune system 3 , 4 . (cdc.gov)

Those results suggest that PRMT1 may be involved in PXR-activated overexpression of MDR1 in resistant breast cancer cells, and AMI-1 may suppress MDR1 by disrupting the interaction between PRMT1 and PXR. (oncotarget.com)
Additionally, chemiluminescence results also showed that the concentration of IGF-1 in the transduced cells significantly increased compared to the control group. (cellmolbiol.org)
Overexpression of miR-26a in glioma primarily results from amplification at the miR-26a-2 locus, a genomic event strongly associated with monoallelic PTEN loss.miR-26a-mediated PTEN repression in a murine glioma model both enhances de novo tumor formation and precludes loss of heterozygosity and the PTEN locus. (wikipedia.org)

Interestingly, acid-sensing ion channel (ASIC) 1, 2a, 2b, and 3 mRNAs were decreased in the DRG, and this reduction was strongest in females. (jneurosci.org)

Human Cripto-1, a member of the EGF-CFC family, is indispensable for early embryonic development. (biomedcentral.com)
At the core of the network reside Oct4, Sox2 and Nanog, into embryonic stem cells [1,2,3,4,5], have made major inroads which form a self-organized core of the TFs maintaining into stem cell biology. (lu.se)

Biochemical analyses revealed a preferential activation of the AKT pathway upon Ig-NRG1 overexpression. (uni-goettingen.de)

The aim of this study was to analyze the roles of Cripto-1 in the progression and clinical characteristics in NPC clinical samples and cell lines. (biomedcentral.com)

Adult-male SD rats (n=32) were equally divided into groups 1 (sham-operated control), 2 (DCM), 3 (DCM + iPS-MSCs/1.2 x 10 6 cells/administered by post-28 day's DCM induction) and 4 (DCM + iPS-MSC dOex-mIRs /1.2 x 10 6 cells) and euthanized by day 60 after DCM induction. (researchsquare.com)

B cell receptor (BCR) signaling plays a critical role in driving proliferation and survival of the malignant clone in chronic lymphocytic leukemia (CLL), supported by the clinical activity of inhibitors targeted toward BCR-associated kinases [ 1 ]. (nature.com)

Therefore, THP-1 is an acute monocytic leukemia cell line commonly used in laboratories. (ubigene.us)
Since acute Chagas' disease involves an inflammatory response followed by chamber dilation, with subsequent compensatory hypertrophy, we hypothesized CT-1 and gp130 may participate in this disease process. (utmb.edu)
These data suggest that there is substantial overexpression of both CT-1 and gp130 in the heart during acute Chagasic carditis. (utmb.edu)

J Cell Biol (1995) 129 (1): 147-156. (rupress.org)

Nine (90%) of 10 with more than a 10‐fold overexpression relative to the corresponding normal tissue showed severe vascular permeation in the histologic specimens. (elsevierpure.com)
Overexpression 8- to 10-fold of normal ovine brain. (cdc.gov)

In a clinicopathologic study, no relationship was observed between the overexpression of EF1γ mRNA and the histologic grading or depth of tumor invasion. (elsevierpure.com)
[ 1 ] (See the histologic sample in the image below. (medscape.com)

Expected and potentially novel resistance genes are enriched in the top hits and are validated using individual sgRNA as well as cDNA overexpression. (cdc.gov)

Detailed analyses of MCT-1 oncogenicity in H1299 p53-null lung cancer cells have shown that ectopically expressed MCT-1 advances xenograft tumorigenicity and angiogenesis, which cannot be completely suppressed by induction of p53. (tmu.edu.tw)

TP53INP1 overexpression in PC seems to be a worse prognostic factor, particularly predictive of biological cancer relapse. (urotoday.com)

IHC showed weak and diffuse staining for CT-1 in control myocardium, while intense staining especially localized to the cytoplasmic region of cardiomyocytes, was found in infected animals. (utmb.edu)

Anatomy25

Organisms14

Diseases13

Chemicals and Drugs99

Analytical, Diagnostic and Therapeutic Techniques and Equipment36

Phenomena and Processes67

Disciplines and Occupations1

Information Science3

Recombinant5

Protein20

Gene13

Tumorigenesis7

Proliferation and migration1

Lentivirus-mediated2

Cyclin4

Expression24

Tissues4

Inhibits2

Differentiation2

Endothelial2

Carcinomas3

Mice11

Induces1

Dilated cardiomyopathy2

Tumors3

Escherichia1

20223

Epithelial1

Metastasis2

Epidermal1

Accelerates1

Moderate3

Markedly1

Insulin1

Mitochondrial4

Phenotype2

ABSTRACT1

Accumulation4

Increases2

Migration1

Biomarker1

Upregulation1

Inhibitor2

Conditional2

Nuclear2

Methods1

Results3

Interestingly1

Embryonic2

Pathway1

Progression1

Rats1

Receptor1

Acute3

Cell Biol1

Normal2

Histologic2

Genes1

Analyses1

Prognostic1

Cytoplasmic1