• Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. (reageno.com)
  • In molecular biology, extracellular signal-regulated kinases (ERKs) or classical MAP kinases are widely expressed protein kinase intracellular signalling molecules that are involved in functions including the regulation of meiosis, mitosis, and postmitotic functions in differentiated cells. (wikipedia.org)
  • citation needed] The term, "extracellular signal-regulated kinases", is sometimes used as a synonym for mitogen-activated protein kinase (MAPK), but has more recently been adopted for a specific subset of the mammalian MAPK family. (wikipedia.org)
  • citation needed] In the MAPK/ERK pathway, Ras activates c-Raf, followed by mitogen-activated protein kinase kinase (abbreviated as MKK, MEK, or MAP2K) and then MAPK1/2 (below). (wikipedia.org)
  • ERKs are known to activate many transcription factors, such as ELK1, and some downstream protein kinases. (wikipedia.org)
  • Mitogen-activated protein kinase 1 (MAPK1) is also known as extracellular signal-regulated kinase 2 (ERK2). (wikipedia.org)
  • Two similar protein kinases with 85% sequence identity were originally called ERK1 and ERK2. (wikipedia.org)
  • They were found during a search for protein kinases that are rapidly phosphorylated after activation of cell surface tyrosine kinases such as the epidermal growth factor receptor. (wikipedia.org)
  • The protein encoded by this gene is a member of the MAP kinase family. (antibodies-online.com)
  • Find Mitogen-Activated Protein Kinase 1 ELISA Kits for a variety of species such as anti-Human Mitogen-Activated Protein Kinase 1, anti-Mouse Mitogen-Activated Protein Kinase 1, anti-Rat Mitogen-Activated Protein Kinase 1. (antibodies-online.com)
  • Find Mitogen-Activated Protein Kinase 1 ELISA Kits with a specific Detection Method. (antibodies-online.com)
  • Find Mitogen-Activated Protein Kinase 1 ELISA Kits with a specific Sample. (antibodies-online.com)
  • Immunoblot analyses with anti-active mitogen-activated protein kinases (MAP-Ks) revealed that D1 receptors, D2 receptors, group I mGluRs, and group II mGluRs all contribute to MAP-K activation in prefrontal cortex, and that combined activation of dopamine receptors and mGluRs synergistically or additively activate MAP-Ks. (jneurosci.org)
  • In humans, interaction with Sur-2 is required for transcription to be activated by the activation domain of a transcription factor of the ETS-family in response to activated mitogen-activated protein (MAP) kinase. (novusbio.com)
  • N-PAC (AAH64940.1, 1 a.a. - 484 a.a.) full-length human protein. (novusbio.com)
  • Antibody reactive against recombinant protein with GST tag on ELISA and western blot and also on transfected lysate in western blot. (novusbio.com)
  • Subsequently the anti-programmed cell death protein 1 (PD-1) antibodies, nivolumab and pembrolizumab were shown to be more effective than ipilimumab. (biomedcentral.com)
  • In melanoma, therapeutic targets include the T cell receptors, cytotoxic T-lymphocyte-associated antigen 4 (CTLA-4) and programmed cell death protein 1 (PD-1) [ 9 ]. (biomedcentral.com)
  • The p38 mitogen-activated protein kinases (p38 MAP kinases) are a family of four related Ser/Thr kinases responsive to pro-inflammatory cytokines and environmental stresses, including ionizing radiation, oxidative stress, and osmotic shock. (rndsystems.com)
  • For p38 beta, also known as p38-2, stress-activated protein kinase 2B (SAPK2B), and MAPK11, this dual phosphorylation occurs at Thr180/Tyr182. (rndsystems.com)
  • There also was an observed increase in the autophosphorylation of Ca2+/calmodulin-dependent protein kinase II (CaM kinase II). (cdc.gov)
  • Its activation is tightly controlled by numerous other signaling proteins including protein kinase C (PKC), Sab/SH3BP, and caveolin-1. (medscape.com)
  • Although intracellular signal transduction is often portrayed as a protein kinase 'domino effect', the counterbalancing function of phosphatases, and thus the control of phosphatase activity, is equally relevant to proper regulation of cellular function. (ac.be)
  • 1 Reversible protein phosphorylation is an important regulatory mechanism that controls the activities of a myriad of proteins and is thus involved in virtually every major physiological process. (ac.be)
  • In the past, most of the attention was focused primarily on protein kinases and on their regulation, mainly because phosphatases were then viewed as simple housekeeping enzymes. (ac.be)
  • But advances in the understanding of protein phosphatases make now clear that these enzymes are precisely regulated and are as important as kinases in the regulation of cellular processes involving protein phosphorylation. (ac.be)
  • 2 Protein phosphatase 2A (PP2A) is a very abundant - it accounts for as much as 1% of total cellular proteins - ubiquitous and remarkably conserved enzyme. (ac.be)
  • SP600125 inhibited interleukin-1β-induced JNK activity and activator protein-1 activation, but it did not affect the activation of extracellular-regulated kinase, p38 mitogen-activated protein kinase, and nuclear factor-κB. (aspetjournals.org)
  • SP600125 inhibited platelet-derived growth factor-induced proliferation, inducible monocyte chemoattractant protein-1 production, and serum-induced type I collagen production. (aspetjournals.org)
  • MCP-1, monocyte chemoattractant protein-1. (aspetjournals.org)
  • VEGF-induced pMF was attenuated by the MEK/ERK inhibitor U0126 [1,4-diamino-2,3-dicyano-1,4-bis( o -aminophenylmercapto)butadiene] and was abolished by the phosphotidinositol 3 kinase/Akt inhibitor LY294002 [2-(4-morpholinyl)-8-phenyl-1(4 H )-benzopyran-4-one hydrochloride], demonstrating that ERK mitogen-activated protein kinases and Akt are both required for full expression of VEGF-induced pMF. (jneurosci.org)
  • Genetic studies suggest that the product of the SUR-2 gene encodes a protein Sur-2, that acts downstream of the Raf/Map kinase pathway. (bdbiosciences.com)
  • Recombinant protein corresponding to aa 1-270 of human IL-1 beta. (bio-rad-antibodies.com)
  • We demonstrate that protein kinase N (PKN) facilitates mTORC1 signalling by repressing PI3KC2-β-mediated phosphatidylinositol-3,4-bisphosphate synthesis downstream of mTORC2. (nature.com)
  • Protein kinase activity was studied in cytosolic extracts from leaves of wild type Arabidopsis thaliana, the ethylene-insensitive mutant, etr1, and the constitutive triple-response mutant, ctr1. (aber.ac.uk)
  • In etr1, constitutive protein kinase activity was lower than in wild type, but in ctr1, activity was enhanced. (aber.ac.uk)
  • A protein of Mr∼47 kDa associated with MBP-phosphorylating activity was detected using in gel protein kinase assays and phosphorylation of this protein was promoted by ethylene treatment in wild type while activity in the mutants reflected that of MBP phosphorylation. (aber.ac.uk)
  • Signal transduction from pathways that involve Janus tyrosine kinases/signal transducer and activator of transcription proteins (Jak1, Jak2, and Tyk2/STAT3, STAT3, and STATG), src-related protein tyrosine kinases (Lyn and Syk), Ras/MAP kinase, and phosphatidylinositol have been reported to be activated upon G-CSF stimulation (1). (rndsystems.com)
  • Additionally, severe hypoxia and ER stress activated ADAM17 and ectodomain shedding of TNFR1 involving mitogen-activated protein (MAP) kinases and reactive oxygen species (ROS). (ox.ac.uk)
  • Members of the MAP kinase family of protein kinases (e.g. p38 and Jnk) are subject to dual phosphorylation of a conserved T-X-Y motif in their activation domain by upstream members of the MAP/ERK kinase (MEK/MKK) family. (enzolifesciences.com)
  • This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. (innov-research.com)
  • Kinexus is a signal transduction company that utilizes innovative strategies to map and exploit intracellular communication systems involving protein kinases for drug discovery and development. (hum-molgen.org)
  • The services include antibody microarrays, peptide arrays, kinase profiling, substrate profiling, mass spectrometry, bioinformatics analysis and standard screens that track the expression levels and phosphorylation states of hundreds of known cell signalling proteins within thematic families of protein kinases, phosphatases, stress/heat shock, cell cycle and apoptosis proteins. (hum-molgen.org)
  • Component of a protein kinase signal transduction cascade. (abcam.cn)
  • Belongs to the protein kinase superfamily. (abcam.cn)
  • STE Ser/Thr protein kinase family. (abcam.cn)
  • Contains 1 protein kinase domain. (abcam.cn)
  • This gene encodes a receptor that belongs to the protein tyrosine kinase Tie2 family. (thearabidopsisbook.org)
  • This study provides evidence that a switch-protein kinase regulatory network controls availability of σ 66 , the main sigma subunit for transcription in Chlamydia . (plos.org)
  • In vitro analysis revealed that a putative switch-protein kinase regulator, RsbW, is capable of interacting directly with σ 66 , as well as phosphorylating its own antagonist, RsbV1, rendering it inactive. (plos.org)
  • Using model-based clustering, we found two major proteomic subtypes of SCLC characterized by either high thyroid transcription factor-1 (TTF1)/low cMYC protein expression or high cMYC/low TTF1. (oncotarget.com)
  • Applying "drug target constellation" (DTECT) mapping, we further show that protein levels of TTF1 and cMYC predict response to targeted therapies including aurora kinase, Bcl2, and HSP90 inhibitors. (oncotarget.com)
  • Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. (arigobio.com)
  • Activated KIT promotes phosphorylation of the protein phosphatases PTPN6/SHP-1 and PTPRU, and of the transcription factors STAT1, STAT3, STAT5A and STAT5B. (arigobio.com)
  • Kinase activity is down-regulated by phosphorylation on serine residues by protein kinase C family members. (arigobio.com)
  • Phosphorylation at Tyr-568 is required for interaction with PTPN11/SHP-2, CRK (isoform Crk-II) and members of the SRC tyrosine-protein kinase family. (arigobio.com)
  • Mathematical modeling of signal transduction networks has previously been used to map out thermodynamical using rate equations is increasingly attracting attention as a properties of protein-folding models (6,7). (lu.se)
  • Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. (abcam.com)
  • Traditional polyclonal and monoclonal antibodies are the product of normal B cell development and genetic recombination. (cellsignal.com)
  • In contrast, hybridoma-based systems for producing monoclonal antibodies are subject to genetic drift and instability, increasing the potential for lot-to-lot variability or loss of antibody expression. (cellsignal.com)
  • Explore our solutions for multiple applications - vaccines, monoclonal antibodies, recombinant proteins, cell or gene therapy. (vwr.com)
  • Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. (map-kinase.com)
  • Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. (abcam.cn)
  • Phosphorylation of ERKs leads to the activation of their kinase activity. (wikipedia.org)
  • The activation of this kinase requires its phosphorylation by upstream kinases. (antibodies-online.com)
  • Tyr is the phosphorylation site that is most tightly linked to oncogenic transformation and coupling to the ras/MAP kinase signaling pathway. (ihcworld.com)
  • Each family member, p38 alpha, p38 beta, p38δ, and p38 gamma, is activated by dual Thr and Tyr phosphorylation within a Thr-Gly-Tyr motif residing in the kinase activation loop. (rndsystems.com)
  • Carbon disulfide inhalation increases Ca2+/calmodulin-dependent kinase phosphorylation of cytoskeletal proteins in the rat central nervous system. (cdc.gov)
  • Exposure to CS2 resulted in increased phosphorylation of endogenous MAP-2 and exogenously added neurofilament triplet proteins. (cdc.gov)
  • The finding of large increases in the autophosphorylation and calmodulin-binding to CaM kinase II with only slight increases in the amount of antibody-binding suggests that CS2 exposure results in increased Ca2+/calmodulin-dependent phosphorylation of proteins by inducing an increase in kinase activity. (cdc.gov)
  • Fig. 1: Serum mitogen-induced phosphorylation of PI3KC2-β induces complex formation with 14-3-3 proteins. (nature.com)
  • MKK3 and MKK6 phosphorylate p38 in response to cytokine stimulation and cellular stress, while MKK7 and MKK4 activate the Jnk kinases by phosphorylation of the threonine and tyrosine residues, respectively. (enzolifesciences.com)
  • An inhibitor of USP13 decreased the K63 polyubiquitination on TRAF6, TAK1 phosphorylation, IL-1β, and TNF-α induction in response to LPS in BMDMs. (life-science-alliance.org)
  • The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. (abcam.cn)
  • These receptors are transmembrane serinethreonine kinases which upon binding of a ligand recruit the style I receptors ALK1, ALK2, ALK3 selleck chemicals or ALK6 for BMPRII and ALK1 or ALK5 for TGFBRII, primary to phosphorylation and activation in the sort I receptor kinases. (sirnalibrary.com)
  • Activates the AKT1 signaling pathway by phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. (arigobio.com)
  • Isoform 1 shows low levels of tyrosine phosphorylation in the absence of added KITLG/SCF (in vitro). (arigobio.com)
  • Phosphorylation at Tyr-570 is required for interaction with PTPN6/SHP-1. (arigobio.com)
  • It has been shown that WISP proteins are upregulated in Wnt-1-transformed cells (Pennica studies suggest loss of WISP-2 signalling may be a crucial permissive event for EMT and ECM degradation and cell migration. (abic2004.org)
  • Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. (bdbiosciences.com)
  • Antibodies to phosphorylated MAPKinase recognised proteins at ∼47 kDa and the signal was upregulated in response to ethylene. (aber.ac.uk)
  • Kinexus has demonstrated that its antibody-based Kinetworks™ screening platform can identify novel cross-reactive proteins, which has led to the identification of potential drug targets for which patents have been filed. (hum-molgen.org)
  • MKP-1 −/− showed an aberrant regulation of several DUBs and increased expression of proteins and genes involved in IL-1/TLR signaling upstream of MAPK, including IL-1R1, IRAK1, TRAF6, phosphorylated TAK1, and an increased K63 polyubiquitination on TRAF6. (life-science-alliance.org)
  • Among abundant DUBs, ubiquitin-specific protease-13 (USP13), which cleaves polyubiquitin-chains on client proteins, was substantially enhanced in murine MKP-1-deficient BMDMs. (life-science-alliance.org)
  • FineTest offers customers a variety of Cardiac Research products with high specificity, sensitivity and purity, namely Elisa kits, Antibodies and Proteins. (fn-test.com)
  • Many proteins have been identified as substrates for the kinase activity of DNA-PK. (dnapk-signaling.com)
  • ERK5 antibody LS-C219117 is a biotin-conjugated rabbit polyclonal antibody to human ERK5 (MAPK7) (aa776-805). (lsbio.com)
  • Western Blot: N-PAC Antibody [H00084656-B01P] - Analysis of N-PAC expression in transfected 293T cell line by N-PAC polyclonal antibody. (novusbio.com)
  • While polyclonal antibodies are secreted by many different B cell clones and recognize multiple antigenic epitopes, monoclonals originate from a single B cell clone and are specific for just one epitope. (cellsignal.com)
  • Where polyclonal antibodies are purified directly from the serum of the immunized host, and monoclonals are purified from either hybridoma-derived tissue culture supernatant or ascites, recombinant antibodies are instead purified from the tissue culture supernatants of transfected host cell lines. (cellsignal.com)
  • Regardless of whether an antibody is polyclonal, monoclonal or recombinant, it must always be properly validated in the intended application prior to experimental use. (cellsignal.com)
  • Standards(Expression system for standard: NSO, Immunogen sequence: V20-T547) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for EphA4 is added subsequently and then followed by washing with PBS or TBS buffer. (innov-research.com)
  • The capture antibody is a monoclonal antibody from rat, the detection antibody is a biotinylated polyclonal antibody from goat. (innov-research.com)
  • The MAP kinase-kinase, which activates ERK, was named "MAPK/ERK kinase" (MEK). (wikipedia.org)
  • Receptor-linked tyrosine kinases, Ras, Raf, MEK, and MAPK could be fitted into a signaling cascade linking an extracellular signal to MAPK activation. (wikipedia.org)
  • MAPK phosphatase (MKP)-1 regulates innate and adaptive immunity. (life-science-alliance.org)
  • 51094-1-AP targets MEK6 in WB, ELISA applications and shows reactivity with human samples. (ptglab.com)
  • In the MyD88-dependent pathway, after detecting pathogen-associated molecular patterns, MyD88 is recruited to TLRs with interleukin 1 receptor-associated kinases (IRAKs) and activates a ubiquitin E3 ligase, the TNF receptor-associated factor 6 (TRAF6) ( Metzger et al, 2014 ). (life-science-alliance.org)
  • The ligand angiopoietin-1 binds to this receptor and mediates a signaling pathway that functions in embryonic vascular development. (thearabidopsisbook.org)
  • Various lysates were subjected to SDS PAGE followed by western blot with 19856-1-AP (PCM1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. (ptglab.com)
  • NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 19856-1-AP (PCM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. (ptglab.com)
  • This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc. (bdbiosciences.com)
  • Ras is typically activated by growth hormones through receptor tyrosine kinases and GRB2/SOS, but may also receive other signals. (wikipedia.org)
  • VEGFA-165 signals by activating its receptor tyrosine kinase VEGF receptor-2 (VEGFR-2). (jneurosci.org)
  • Because another growth/trophic factor that signals via a receptor tyrosine kinase (brain derived neurotrophic factor) elicits a long-lasting facilitation of respiratory motor activity in the phrenic nerve, we tested the hypothesis that VEGFA-165 elicits similar phrenic motor facilitation (pMF). (jneurosci.org)
  • A framework for extremely multiplexed dextramer mapping and prediction of T cell receptor sequences to antigen specificity T cell receptor (TCR) antigen-specific recognition is important for the adaptive immune system. (medmk.com)
  • Binding of G-CSF to its receptor induces dimerization or oligomerization of the receptor activating cytoplasmic tyrosine kinases. (rndsystems.com)
  • HSCs expressing the receptor tyrosine kinase TIE2 are quiescent and antiapoptotic and comprise a side population of HSCs that adhere to osteoblasts in the bone marrow niche. (thearabidopsisbook.org)
  • Conditional deletion of Mapk1 in B cells showed a role for MAPK1 in T-cell-dependent antibody production. (wikipedia.org)
  • To order the Mouse MAPK1/MAPK3 Monoclonal Antibody (Cat. (map-kinase.com)
  • Activated KIT also transmits signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. (arigobio.com)
  • The exact immunogen sequence used to generate this antibody is proprietary information. (abcam.com)
  • If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. (abcam.com)
  • MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. (antibodies-online.com)
  • At CST, we adhere to the Hallmarks of Antibody Validation™ , six complementary strategies for determining the specificity, sensitivity, and functionality of an antibody in any given assay. (cellsignal.com)
  • We describe areas where major inroads were initially achieved by targeting angiogenesis and by unraveling pathways in the heterogeneous tumors of mesenchymal origin-spurred by the identification of c-Kit-activating mutations in GIST and the regressions that ensued when tumors harboring these mutations were exposed to the tyrosine kinase inhibitor imatinib (Gleevec). (cancernetwork.com)
  • Here, in Part I, we describe areas where major inroads were initially achieved by targeting angiogenesis (central to the biology of renal cell carcinoma and hepatocellular cancer) and by unraveling pathways in the heterogeneous tumors of mesenchymal origin-spurred by the identification of c-Kit-activating mutations in gastrointestinal stromal tumors (GIST) and the regressions that ensued when tumors harboring these mutations were exposed to the tyrosine kinase inhibitor imatinib (Gleevec). (cancernetwork.com)
  • This disorder is now formally referred to as X-linked agammaglobulinemia (XLA), and the gene defect has been mapped to the gene that codes for Bruton tyrosine kinase (Btk) at band Xq21.3. (medscape.com)
  • The BTK gene is large and consists of 19 exons that encode the 659 amino acids that form the Btk cytosolic tyrosine kinase. (medscape.com)
  • Bruton's tyrosine kinase (Btk) is a member of the Tec family of kinases. (medscape.com)
  • Description: TIE2, also known as TEK tyrosine kinase, is mapped to 9p21.2. (thearabidopsisbook.org)
  • The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. (bdbiosciences.com)
  • This MAPK7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 776-805 amino acids from the C-terminal region of human MAPK7. (lsbio.com)
  • LTD by dopamine + 1 S ,3 R -ACPD coapplication was also blocked by postsynaptic injection of synthetic MAP-K substrate peptide. (jneurosci.org)
  • A synthetic peptide that maps to a region between residues 1 and 50 of human Cofactor Required for Sp1 transcriptional activation, subunit 3 using the numbering given in Swiss-Prot entry Q9ULK4 (GeneID 9439). (novusbio.com)
  • You can block the antibody by the specific target amino acid sequence of peptide. (blockingantibody.com)
  • Kinexus currently offers products including high quality antibody micorarrays, peptides, peptide arrays and antibodies, as well as services relating to signal transduction. (hum-molgen.org)
  • The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. (ptglab.com)
  • This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. (reageno.com)
  • Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 19856-1-AP (PCM1 antibody) at dilution of 1:200 (under 10x lens). (ptglab.com)
  • Optimal dilution of this antibody should be experimentally determined. (novusbio.com)
  • Dilution: 1:20 using IHC-Tek TM Antibody Diluent (Cat# IW-1000 or IW-1001) to reduce background and unspecific staining and serum blocking step is NOT needed. (ihcworld.com)
  • Consistently, LTD by dopamine + 1 S ,3 R -ACPD coapplication, as well as the two other forms of LTD (LTD by dopamine + tetani and LTD by 1S,3R-ACPD + tetani), was blocked by bath application of MAP-K kinase inhibitor PD98059. (jneurosci.org)
  • We here evaluated the effects of SP600125 (anthra[1,9- cd ]pyrazole-6 (2 H )-one), an inhibitor of c-Jun NH 2 -terminal kinase (JNK), on the activation of PSCs. (aspetjournals.org)
  • 1.12?inhibitor STK870702, the migration distance of HGC27 WISP-2 kd cells (Resistance) decreased significantly as the concentration increased from 0.112 to 1 1.125?inhibitor did not change the migration distance of pEF cells significantly at any concentrations (*(B) and JNK (C) pathways. (abic2004.org)
  • Mouse anti Human IL-1 beta, clone 2E8 recognizes human interleukin-1β, also known as catabolin. (bio-rad-antibodies.com)
  • The primary data for 1-step growth, 2-step growth and gene expression summarized in Figs 4 and 5 are available in the S1 Dataset in the supplementary information. (plos.org)
  • PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human/Mouse/Rat p38 beta Monoclonal Antibody (Catalog # MAB5885) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007 ). (rndsystems.com)
  • Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor ® 488 Conjugate) #4412 was used as a secondary antibody. (cellsignal.com)
  • At Proteintech, we pride ourselves on our antibody quality, customer service and transparency. (ptglab.com)
  • FineTest is a professional biotech research laboratory reagent manufacturer equipped with advanced Elisa test research lab, offering about 7,000 types Elisa kits and 10,000 types Antibodies with stable product quality as well available PubMed publications. (fn-test.com)
  • See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more. (lsbio.com)
  • Human IgG antibody Laboratories manufactures the tissue tek cryomold biopsy sakura reagents distributed by Genprice. (thearabidopsisbook.org)
  • Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. (antibodies-online.com)
  • Our results suggest that dopamine receptors and groups I and II mGluRs cooperate to induce LTD through converging postsynaptic activation of MAP-Ks. (jneurosci.org)
  • Regulates p38 MAP kinase activity by mediating stress activation ofp38alpha/MAPK14 and specifically regulating MAPK14 signaling. (novusbio.com)
  • Activation of JNK was determined by Western blotting using anti-phosphospecific JNK and c-Jun antibodies. (aspetjournals.org)
  • Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. (arigobio.com)
  • TTF1 (used in the diagnosis lung cancer) could therefore be used as a surrogate of DLL3 expression to identify patients who may respond to the DLL3 antibody-drug conjugate rovalpituzumab tesirine. (oncotarget.com)
  • However, murine models, including knockout and transgenic mice, have been useful in understanding the mechanisms of B lymphopoiesis, B-cell differentiation, and antibody formation. (medscape.com)
  • In the next series of experiments, coapplication of dopamine and 1 S ,3 R -ACPD, but not application of either drug alone, consistently induced LTD without tetani or even single test stimuli during drug application, suggesting that coactivation of dopamine receptors and the mGluRs is sufficient for LTD induction. (jneurosci.org)
  • Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. (innov-research.com)
  • Recombinant antibodies are monoclonal, but their production involves in vitro genetic manipulation. (cellsignal.com)
  • In vitro methods for producing antibodies are amenable to large-scale production, meaning antibody availability is unlikely to become a limiting factor. (cellsignal.com)
  • Slight increases in the binding of a monoclonal antibody to the a subunit of CaM kinase II were seen, while large increases in the binding of [125Icalmodulin to the a subunit of CaM kinase II also were observed. (cdc.gov)
  • Nonetheless, constructing a TCR-antigen interplay map has been difficult because of the staggering range of TCRs and antigens. (medmk.com)
  • Immunocytochemistry/ Immunofluorescence: N-PAC Antibody [H00084656-B01P] - Analysis of purified antibody to N-PAC on HeLa cell. (novusbio.com)
  • Supplementary MaterialsAdditional file 1: Number S1 Immunohistochemical analysis of KIF2A in breast cancer tissues showing different intensity grade, (A) Weak, (B) moderate and (C) strong. (forgetmenotinitiative.org)