Antifreeze Proteins: Proteins that bind to ice and modify the growth of ice crystals. They perform a cryoprotective role in a variety of organisms.Antifreeze Proteins, Type I: A subclass of ANTIFREEZE PROTEINS that are 3-5 kDa in size and contain a single alanine-rich amphipathic alpha-helix.Antifreeze Proteins, Type III: A subclass of ANTIFREEZE PROTEINS that are globular, 6.5 kDa in size and contain compact beta-sheet structures.Ice: The solid substance formed by the FREEZING of water.Flounder: Common name for two families of FLATFISHES belonging to the order Pleuronectiformes: left-eye flounders (Bothidae) and right-eye flounders (Pleuronectidae). The latter is more commonly used in research.Freezing: Liquids transforming into solids by the removal of heat.Antifreeze Proteins, Type IV: A subclass of ANTIFREEZE PROTEINS that contain four amphipathic alpha-helices folded into an antiparallel helix bundle.Antifreeze Proteins, Type II: A subclass of ANTIFREEZE PROTEINS that have a cystine-rich globular structure of approximately 14 kD.Tenebrio: A genus of beetles which infests grain products. Its larva is called mealworm.Fishes: A group of cold-blooded, aquatic vertebrates having gills, fins, a cartilaginous or bony endoskeleton, and elongated bodies covered with scales.Osmeriformes: An order of fish including smelts, galaxiids, and salamanderfish.Fish Proteins: Proteins obtained from species of fish (FISHES).Marinomonas: A genus of gram-negative, aerobic bacteria in the family OCEANOSPIRILLACEAE. Members of this genus have the ability to synthesize MELANIN pigments.Antarctic Regions: The continent lying around the South Pole and the southern waters of the Atlantic, Pacific, and Indian Oceans. It includes the Falkland Islands Dependencies. (From Webster's New Geographical Dictionary, 1988, p55)Eels: Common name for an order (Anguilliformes) of voracious, elongate, snakelike teleost fishes.Glycoproteins: Conjugated protein-carbohydrate compounds including mucins, mucoid, and amyloid glycoproteins.Flatfishes: Common name for the order Pleuronectiformes. A very distinctive group in that during development they become asymmetrical, i.e., one eye migrates to lie adjacent to the other. They swim on the eyeless side. FLOUNDER, sole, and turbot, along with several others, are included in this order.Salmoniformes: An order of fish comprising salmons, trouts, whitefish, graylings, and other families. They are both marine and freshwater fish, found in all oceans and are quite numerous in the Northern Hemisphere. (From Nelson: Fishes of the World)Secale cereale: A hardy grain crop, rye, grown in northern climates. It is the most frequent host to ergot (CLAVICEPS), the toxic fungus. Its hybrid with TRITICUM is TRITICALE, another grain.Beetles: INSECTS of the order Coleoptera, containing over 350,000 species in 150 families. They possess hard bodies and their mouthparts are adapted for chewing.Cold Climate: A climate characterized by COLD TEMPERATURE for a majority of the time during the year.Daucus carota: A plant species of the family APIACEAE that is widely cultivated for the edible yellow-orange root. The plant has finely divided leaves and flat clusters of small white flowers.Snow: Frozen water crystals that fall from the ATMOSPHERE.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Crystallization: The formation of crystalline substances from solutions or melts. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Protein Structure, Secondary: The level of protein structure in which regular hydrogen-bond interactions within contiguous stretches of polypeptide chain give rise to alpha helices, beta strands (which align to form beta sheets) or other types of coils. This is the first folding level of protein conformation.Cold Temperature: An absence of warmth or heat or a temperature notably below an accustomed norm.Perciformes: The most diversified of all fish orders and the largest vertebrate order. It includes many of the commonly known fish such as porgies, croakers, sunfishes, dolphin fish, mackerels, TUNA, etc.Neutron Diffraction: The scattering of NEUTRONS by matter, especially crystals, with accompanying variation in intensity due to interference effects. It is useful in CRYSTALLOGRAPHY and POWDER DIFFRACTION.Models, Molecular: Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures.Gadiformes: An order of fish including the families Gadidae (cods), Macrouridae (grenadiers), and hakes. The large Gadidae family includes cod, haddock, whiting, and pollock.Siphonaptera: An order of parasitic, blood-sucking, wingless INSECTS with the common name of fleas.Acclimatization: Adaptation to a new environment or to a change in the old.Circular Dichroism: A change from planar to elliptic polarization when an initially plane-polarized light wave traverses an optically active medium. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Protein Conformation: The characteristic 3-dimensional shape of a protein, including the secondary, supersecondary (motifs), tertiary (domains) and quaternary structure of the peptide chain. PROTEIN STRUCTURE, QUATERNARY describes the conformation assumed by multimeric proteins (aggregates of more than one polypeptide chain).Insect Proteins: Proteins found in any species of insect.Solutions: The homogeneous mixtures formed by the mixing of a solid, liquid, or gaseous substance (solute) with a liquid (the solvent), from which the dissolved substances can be recovered by physical processes. (From Grant & Hackh's Chemical Dictionary, 5th ed)Cryopreservation: Preservation of cells, tissues, organs, or embryos by freezing. In histological preparations, cryopreservation or cryofixation is used to maintain the existing form, structure, and chemical composition of all the constituent elements of the specimens.Camphor: A bicyclic monoterpene ketone found widely in plants, especially CINNAMOMUM CAMPHORA. It is used topically as a skin antipruritic and as an anti-infective agent.Cryoprotective Agents: Substances that provide protection against the harmful effects of freezing temperatures.Databases, Protein: Databases containing information about PROTEINS such as AMINO ACID SEQUENCE; PROTEIN CONFORMATION; and other properties.Internet: A loose confederation of computer communication networks around the world. The networks that make up the Internet are connected through several backbone networks. The Internet grew out of the US Government ARPAnet project and was designed to facilitate information exchange.User-Computer Interface: The portion of an interactive computer program that issues messages to and receives commands from a user.Genomics: The systematic study of the complete DNA sequences (GENOME) of organisms.Sciuridae: A family of the order Rodentia which contains 49 genera. Some of the more common genera are MARMOTA, which includes the marmot and woodchuck; Sciurus, the gray squirrel, S. carolinensis, and the fox squirrel, S. niger; Tamias, the eastern and western chipmunk; and Tamiasciurus, the red squirrel. The flying squirrels, except the scaly-tailed Anomaluridae, also belong to this family.Hibernation: The dormant state in which some warm-blooded animal species pass the winter. It is characterized by narcosis and by sharp reduction in body temperature and metabolic activity and by a depression of vital signs.Developmental Biology: The field of biology which deals with the process of the growth and differentiation of an organism.Newfoundland and Labrador: Province of Canada consisting of the island of Newfoundland and an area of Labrador. Its capital is St. John's.National Academy of Sciences (U.S.): A United States organization of distinguished scientists and engineers established for the purpose of investigating and reporting upon any subject of art or science as requested by any department of government. The National Research Council organized by NAS serves as the principal operating agency to stimulate and support research.Health Planning Technical Assistance: The provision of expert assistance in developing health planning programs, plans as technical materials, etc., as requested by Health Systems Agencies or other health planning organizations.