Simultaneous determination of seven compounds in snow lotus herb using high-performance liquid chromatography.
A reversed-phase high-performance liquid chromatographic method is developed for the quantitative determination of seven components of Snow Lotus Herb (Saussurea tridactyla Sch.-Bip.ex Hook.f.): umbelliferonglucoside, luteolin-7-O-beta-D-glucoside, rutin, apigenin-7-O-beta-D-glucoside, kaempferol-3-O-beta-D-glucoside, apigenin-7-O-beta-D-rutinoside, and luteolin. Samples are analyzed by means of a reverse-phase column (Diamonsil C(18)) using methanol and water under gradient conditions as the mobile phase for 60 min. This method offers selectivity, accuracy, precision, linearity, and ruggedness, as well as efficiency and ease. (+info)
Induction of apoptosis by Saussurea lappa and Pharbitis nil on AGS gastric cancer cells.
We performed this study to understand the molecular basis underlying the antitumor effects of Saussurea lappa, Pharbitis nil, Plantago asiatica and Taraxacum mongolicum, which have been used for herbal medicinal treatments against cancers in East Asia. We analyzed the effects of these medicinal herbs on proliferation and on expression of cell growth/apoptosis related molecules, with using an AGS gastric cancer cell line. The treatments of Saussurea lappa and Pharbitis nil dramatically reduced cell viabilities in a dose and time-dependent manner, but Plantago asiatica and Taraxacum mongolicum didn't. FACS analysis and Annexin V staining assay also showed that both Saussurea lappa and Pharbitis nil induce apoptotic cell death of AGS. Expression analyses via RT-PCR and Western blots revealed that Saussurea lappa, but not Pharbitis nil, increased expression of the p53 and its downstream effector p21Waf1, and that the both increased expression of apoptosis related Bax and cleavage of active caspase-3 protein. We also confirmed the translocation of Bax to mitochondria. Collectively, our data demonstrate that Saussurea lappa and Pharbitis nil induce growth inhibition and apoptosis of human gastric cancer cells, and these effects are correlated with down- and up-regulation of growth-regulating apoptotic and tumor suppressor genes, respectively. (+info)
Two new sesquiterpene lactones with the sulfonic acid group from Saussurea lappa.
Two new sesquiterpene lactones with the unusual sulfonic acid group, 13-sulfo-dihydrosantamarine (1) and 13-sulfo-dihydroreynosin (2), have been isolated from the roots of Saussurea lappa C. Their structures, including the absolute configurations, were elucidated by spectroscopic methods. (+info)
Human-induced dwarfing of Himalayan snow lotus, Saussurea laniceps (Asteraceae).
Because evolutionary processes such as genetic drift and natural selection play a crucial role in determining the response that species will have to human-induced disturbances, there is increasing interest in the evolutionary aspects of conservation biology. Harvesting select individuals in natural plant populations can bring about unforeseen impacts that may negatively affect fitness. We analyzed how human harvesting affects two congeners known as snow lotus. Over a period of 100 years, there was a negative trend in plant height (r2= 0.4361, P < 0.001) for the intensely collected and rare species, Saussurea laniceps, but not in the less intensely collected species, Saussurea medusa. Additionally, S. laniceps were significantly smaller in areas of high harvest than in areas with low harvest (Z = 4.91, P < 0.0001), but this was not so for S. medusa. Humans can unconsciously drive evolution and must be considered when managing threatened species. (+info)
Antiinflammatory and analgesic activities of the tissue culture of Saussurea involucrata.
The tissue culture of Saussurea involucrata (TCSauI) KAR. et KIR. was studied to determine its antiinflammatory and analgesic activities in experimental animals. Similar to wild S. involucrata, TCSauI at doses of 75-300 mg/kg i.g. for 7 d markedly inhibited hindpaw edema induced by carrageenin in rats, ear edema induced by dimethylbenzene, and increased capillary permeability in the mouse abdominal cavity induced by acetic acid. Moreover, TCSauI had inhibitory activities against the writhing reaction induced by acetic acid and the hot plate reaction in mice. The present study provided evidence first that TCSauI has antiinflammatory and analgesic activities, suggesting the potential of the tissue culture technique to substitute for wild S. involucrata in the pharmaceutical industry. (+info)
Bioactive constituents from Chinese natural medicines. XV. Inhibitory effect on aldose reductase and structures of Saussureosides A and B from Saussurea medusa.
The 80% aqueous acetone extract from the whole plant of Saussurea medusa MAXIM. was found to inhibit rat lens aldose reductase (IC50=1.4 microg/ml). From this extract, flavonoids, lignans, and quinic acid derivatives were isolated together with two new ionone glycosides, saussureosides A and B. Their absolute stereostructures were elucidated on the basis of chemical and physicochemical evidence including the application of modified Mosher's method. In addition, some isolates were found to show an inhibitory effect on aldose reductase. (+info)
Acylated flavone glycosides from the roots of Saussurea lappa and their antifungal activity.
The isolation of four novel acylated flavonoid glycosides from the roots of Saussurea lappa and their identification using a combination of 1D and 2D NMR and mass spectrometry is described. The in vitro antifungal and antibacterial activities of the isolated compounds and their mixture were tested on nine fungal and four bacterial strains, using the microdilution method. The compounds and mixture showed moderate to high antifungal activity against most of the fungi tested, compared to a miconazole standard, while only one compound and the mixture showed antibacterial activity against all strains tested. (+info)
A new sesquiterpene glycoside from the aerial parts of Saussurea triangulata.
Column chromatographic separation of a MeOH extract of the aerial parts of Saussurea triangulata led to the isolation of a new sesquiterpene glycoside 6, together with three quinic acid derivatives, two phenolics, two sesquiterpene glycosides and two flavonoids. The new compound 6 was identified as amarantholidol A glycoside by spectroscopic and chemical methods. (+info)