Prevalence, comorbidities and risk factors of restless legs syndrome in the Korean elderly population - results from the Korean Longitudinal Study on Health and Aging.
(35/2348)
A survey of intestinal protozoan infections among gastroenteritis patients during a 3-year period (2004-2006) in Gyeonggi-do (province), South Korea.
(36/2348)
Strain typing of Mycobacterium tuberculosis isolates from Korea by mycobacterial interspersed repetitive units-variable number of tandem repeats.
(38/2348)
Clinical and pathological findings in experimental brucellosis in pregnant rats.
(39/2348)
BACKGROUND: To investigate the clinical findings and pathological changes in female Sprague-Dawley rats (SD) after experimental infection at late stage of pregnancy with Brucella abortus biotype 1 Korean isolate. METHODOLOGY: Twenty-five rats were used and the rats were classified into two groups, an infected group (n=15) and a control group (n=10). At 18 days of gestation 500 microliters containing 1.0 x 10(9) colony forming unit (CFU) suspension of B. abortus biotype 1 Korean isolate in physiological saline solution was injected subcutaneously to each of 15 rats, and ten rats were injected with only 500 microliters of physiological saline. The SD rats were examined clinically and the spleen, lymph nodes, uterus and placenta were examined grossly and microscopically. Additionally these organs as well as the blood were cultured bacteriologically. RESULTS: There were no stillbirths, abortions or premature births in any of the SD rats. The gross signs of all the SD rats of the infected group included splenomegaly, metritis, enlargement of lymph nodes and placentitis. Moreover, B. abortus biotype 1 were detected in the organs of infected SD rats as well as in the blood. The microscopic signs of the SD rats of the infected group included infiltrations of macrophages, giant cells and engorged macrophages scattered in necrotic debris in the lymph nodes. In the spleen, there was diffuse congestion of the red pulp, diffuse infiltration of macrophages with increased giant cell numbers and prominent germinal centres. In the uterus, there was moderate, diffuse, but multifocally prominent accumulation of lymphocytes and macrophages in the superficial lamina. In the placenta, there were areas of necrosis in the periplacentomal chorionic epithelium. CONCLUSIONS: It is concluded that the Korean pathogenic isolate B. abortus biotype 1 does not induce abortion in SD rats. (+info)
Diagnostic efficacy of Brucella abortus strain RB51 in experimentally inoculated Sprague-Dawley rats using western blot assay.
(40/2348)
BACKGROUND: To investigate the diagnosis and efficacy of Brucella abortus strain RB51 (SRB51) in experimentally inoculated Sprague-Dawley (SD) rat using western blot assay. METHODOLOGY: Female SD rats were orally administered with 1.0 x 10(7) colony forming unit (cfu) suspension of SRB51 and half of these SD rats were challenged at 4 weeks post inoculation with 1.0 x 10(9) cfu suspension of B. abortus biotype 1 isolated in South Korea. Sera of SD rats were monitored at regular intervals by western blot assay using whole cell antigen of B. abortus strain 1119-3 (S1119-3). The bacteriological examination of blood and clinical examination of the rats were also performed. RESULTS: There were several bands at 120, 70, 45, 30, 20 kDa and clear specific bands were found after vaccination (20, 70 kDa) and challenge (15, 20, 45, 70, 120 kDa). The highest immune response was observed in sera 4 weeks post SRB51 vaccination. SRB51 was recovered from the blood of all of SRB51 inoculated rats until one week post vaccination and there were no clinical signs in that inoculated rats. CONCLUSIONS: It is concluded that the SRB51 elicits antigen specific immunity in SD rats based on western blot assay. (+info)