Effects of surface nano-topography on human osteoblast filopodia.
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Anchorage-dependent cells growing over a substratum require stable adhesion areas on the surface for the next cellular activities. The adhesion is achieved by some contact points called focal adhesions. Because focal adhesions were distributed randomly, a trial to control the positions of focal adhesion with a specific order may cause interesting effects like as cytoskeleton rearrangement, which may induce and transfer new signals to the nucleus. Here, we cultured human osteoblasts over two sorts of nanopatterned surfaces with different pattern densities fabricated by using laser interference lithography and the nanoimprinting technique. Of the two nanopatterns, cells over the nanopattern with low pattern density showed relatively higher adaptation to the topography with guided filopodia protrusion. However, cells over the dense nanopattern showed difficulty in finding suitable paths for migration, as judged from the activities of filopodium formation and the presence of a shovel-like feature at the tip of each filopodium. (+info)
A glucose sensor fabricated by piezoelectric inkjet printing of conducting polymers and bienzymes.
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Piezoelectric inkjet printing of polymers and proteins holds great promise for fabrication of miniaturized bioelectronic devices, such as biochips and biosensors. In this study, a bienzymatic glucose biosensor prototype based on poly(3,4-ethylenedioxythiophene)-poly(styrene sulfonic acid) (PEDOT-PSS), glucose oxidase (GOD), and horseradish peroxidase (HRP) was fabricated by a piezoelectric inkjet printer. An aqueous bioelectrical ink containing PEDOT-PSS, GOD, and HRP was prepared and printed on an indium-tin-oxide (ITO)-coated poly(ethylene terephthalate) (PET) film. The PEDOT-PSS/GOD/HRP sensor was covered with a cellulose acetate membrane. The use of bienzymatic sensing combined with conducting polymers via piezoelectric inkjet printing showed a synergistic effect resulting in significant amplification of the response signal. The glucose sensor reached steady-state current density within 3 s, indicating a fast response time, and exhibited a linear dose-dependent electrochemical response with high sensitivity. The overall result demonstrates that a glucose sensor with high sensitivity could be readily fabricated by a piezoelectric inkjet printing system. (+info)
Conductive AFM patterning on oligo(ethylene glycol)-terminated alkyl monolayers on silicon substrates: proposed mechanism and fabrication of avidin patterns.
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