Effect of three commercial mouth rinses on cultured human gingival fibroblast: an in vitro study.
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AIM: To examine the effect of three commercial mouth rinses (Hexidine 0.2%, Listerine Cool Mint, Betadine 1%) upon cultured human gingival fibroblast proliferation. MATERIALS AND METHODS: Human gingival fibroblasts were cultured and incubated in Dulbecco's Minimum Eagle's Medium containing Chlorhexidine, Listerine, Povidone-Iodine at varying concentrations (1%, 2%, 5%, 10%, 20% and 100% of the given solution) at 37 degrees C for 1, 5 and 15 min. Control cells received an equal volume of Dulbecco's Minimum Eagle's Medium without adding mouth rinses, for similar duration of exposure at 37 degrees C. Following incubation the media were removed, cells were washed twice with medium, supplemented with 10% Fetal Bovine Serum, and fibroblasts in the test and control group were allowed to recover in the same media for 24 h. RESULTS: In all the three groups, the proliferation inhibition was dependent on the concentration of solublized mouth rinses in the cell culture but independent of the duration of exposure to all three mouth rinses. The results showed that all three solutions were toxic to cultured human gingival fibroblasts, Chlorhexidine being the most cytotoxic. It was seen that at dilute concentrations (1% and 2% of given solutions) Listerine was more cytotoxic than Chlorhexidine and Povidone-Iodine. CONCLUSION: These results suggest that Chlorhexidine, Listerine and Povidone-Iodine are capable of inducing a dose-dependent reduction in cellular proliferation of fibroblasts. The results presented are interesting, but to know the clinical significance, further studies are needed. (+info)
Should additional antibiotics or an iodine washout be given to all patients who suffer an emergency re-sternotomy on the cardiothoracic intensive care unit?
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Spectrophotometric determination of iodine species in table salt and pharmaceutical preparations.
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A sensitive spectrophotometric method for the determination of iodine species like iodide, iodine, iodate and periodate is described. The method involves the oxidation of iodide to ICl(2)(-) in the presence of iodate and chloride in acidic medium. The formed ICl(2)(-) bleaches the dye methyl red. The decrease in the intensity of the colour of the dye is measured at 520 nm. Beer's law is obeyed in the concentration range 0-3.5 microg of iodide in an overall volume of 10 ml. The molar absorptivity of the colour system is 1.73 x 10(5) l mol(-1) cm(-1) with a correlation coefficient of -0.9997. The relative standard deviation is 3.6% (n=10) at 2 microg of iodide. The developed method can be applied to samples containing iodine, iodate and periodate by prereduction to iodide using Zn/H(+) or NH(2)NH(2)/H(+). The effect of interfering ions on the determination is described. The proposed method has been successfully applied for the determination of iodide and iodate in salt samples and iodine in pharmaceutical preparations. (+info)
Fluoroscopy guided instillation therapy in chyluria using combination of povidone iodine with contrast agent. Is a single instillation sufficient?
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PURPOSE: To evaluate the safety and efficacy of a single instillation in a combination of povidone iodine with contrast agent under fluoroscopy guidance for the treatment of chyluria. MATERIALS AND METHODS: From December 1999 to July 2006 a total of 40 patients with chyluria were treated by renal pelvic instillation therapy (RPIS). The sclerosing solution was prepared using povidone iodine with contrast agent diluted with sterile water in a ratio of 1:1:3. It was instilled on the side having chylous efflux using a bulb tip ureteric catheter. Unilateral instillation was done in 26 cases, 10 on the right side and 16 on left. Fourteen patients had bilateral chylous efflux and RPIS was performed on both sides in the same session. Fluoroscopy was used to evaluate the complete filling of the pelvic calyceal system. The sclerosing solution was kept in the system for 5 minutes and the ureteric catheter was then withdrawn. RESULTS: Immediate clearance was observed in 39 patients. Recurrence occurred in five patients. They were treated again using the same procedure with satisfactory results. The longest follow-up was five years and the shortest five months. CONCLUSION: RPIS of chyluria using a single instillation a combination of povidone iodine with contrast agent is safe and effective. Use of fluoroscopy helps to determine the exact amount of sclerosing solution required to completely fill the system and therefore overfilling is avoided. Moreover, the complications, which arise due to pyelointerstitial backflow, are prevented. (+info)
A comparative study between honey and povidone iodine as dressing solution for Wagner type II diabetic foot ulcers.
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Honey dressing has been used to promote wound healing for years but scanty scientific studies did not provide enough evidences to justify it benefits in the treatment of diabetic foot ulcers. We conducted a prospective study to compare the effect of honey dressing for Wagner's grade-II diabetic foot ulcers with controlled dressing group (povidone iodine followed by normal saline). Surgical debridement and appropriate antibiotics were prescribed in all patients. There were 30 patients age between 31 to 65-years-old (mean of 52.1 years). The mean healing time in the standard dressing group was 15.4 days (range 9-36 days) compared to 14.4 days (range 7-26 days) in the honey group (p < 0.005). In conclusion, ulcer healing was not significantly different in both study groups. Honey dressing is a safe alternative dressing for Wagner grade-II diabetic foot ulcers. (+info)
Isopropyl alcohol compared with isopropyl alcohol plus povidone-iodine as skin preparation for prevention of blood culture contamination.
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The effect of povidone-iodine and chlorhexidine mouth rinses on plaque Streptococcus mutans count in 6- to 12-year-old school children: an in vivo study.
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OBJECTIVES: Treating a carious tooth in children with high caries experience by providing a restoration does not cure the disease. If the unfavorable oral environment that caused the cavity persists so will the disease and more restorations will be required in future. Treating the oral infection by reducing the number of cariogenic microorganisms and establishing a favorable oral environment to promote predominantly remineralization of tooth structure over time will stop the caries process. The present study was conducted: (1) To evaluate the efficacy of povidone-iodine and chlorhexidine mouth rinses on plaque Streptococcus mutans when used as an adjunct to restoration. (2) To compare the anti-microbial effect of 1% povidone-iodine and 0.2% chlorhexidine mouth rinses on plaque S. mutans count. STUDY DESIGN: Forty-five study participants in the age group of 6-12 years with dmft (decay component) of three or four were selected from one government school in Bangalore city. They were divided into three groups after the restorative treatment. Group-A, Group-B, and Group-C received 1% povidone-iodine mouth rinse, 0.2% chlorhexidine mouth rinse and placebo mouth rinse, respectively, twice daily for 14 days. The plaque sample was collected and S. mutans count was estimated at six phases: (1) Baseline, (2) 3 weeks after restoration, (3) First day after mouth rinse therapy, (4) 15 days after mouth rinse therapy, (5) 1 month and (6) 3 months after mouth rinse therapy RESULTS: After the restoration the percentage change in S. mutans count was 28.4%. Immediately after mouth rinse therapy there was significant reduction in S. mutans count in all the three groups. After which the count started to increase gradually and after 3 months the bacterial counts in the povidone-iodine group and placebo group were almost near the postrestorative count. CONCLUSION: Mouth rinses can be used as adjunct to restoration for short duration as temporary measure in reduction of S. mutans count and restorations provide longer effect. In case a mouth rinse has to be used, chlorhexidine can be recommended as it has shown to have better effect than Povidone-iodine and placebo. (+info)