The sources, fate, and toxicity of chemical warfare agent degradation products.
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We include in this review an assessment of the formation, environmental fate, and mammalian and ecotoxicity of CW agent degradation products relevant to environmental and occupational health. These parent CW agents include several vesicants: sulfur mustards [undistilled sulfur mustard (H), sulfur mustard (HD), and an HD/agent T mixture (HT)]; nitrogen mustards [ethylbis(2-chloroethyl)amine (HN1), methylbis(2-chloroethyl)amine (HN2), tris(2-chloroethyl)amine (HN3)], and Lewisite; four nerve agents (O-ethyl S-[2-(diisopropylamino)ethyl] methylphosphonothioate (VX), tabun (GA), sarin (GB), and soman (GD)); and the blood agent cyanogen chloride. The degradation processes considered here include hydrolysis, microbial degradation, oxidation, and photolysis. We also briefly address decontamination but not combustion processes. Because CW agents are generally not considered very persistent, certain degradation products of significant persistence, even those that are not particularly toxic, may indicate previous CW agent presence or that degradation has occurred. Of those products for which there are data on both environmental fate and toxicity, only a few are both environmentally persistent and highly toxic. Major degradation products estimated to be of significant persistence (weeks to years) include thiodiglycol for HD; Lewisite oxide for Lewisite; and ethyl methyl phosphonic acid, methyl phosphonic acid, and possibly S-(2-diisopropylaminoethyl) methylphosphonothioic acid (EA 2192) for VX. Methyl phosphonic acid is also the ultimate hydrolysis product of both GB and GD. The GB product, isopropyl methylphosphonic acid, and a closely related contaminant of GB, diisopropyl methylphosphonate, are also persistent. Of all of these compounds, only Lewisite oxide and EA 2192 possess high mammalian toxicity. Unlike other CW agents, sulfur mustard agents (e.g., HD) are somewhat persistent; therefore, sites or conditions involving potential HD contamination should include an evaluation of both the agent and thiodiglycol. (+info)
The precautionary principle and scientific research are not antithetical.
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The Precautionary Principle is intended to protect human health and the environment. To serve these goals effectively, precautionary action must be coupled with concurrent research to decide whether the action taken is in fact protective. (+info)
Closer to a compromise on the direction of environmental research.
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The Committee for the National Institute for the Environment (CNIE) was created in 1990 "to improve the scientific basis for making decisions on environmental issues," possibly through the establishment of a separate institute devoted to the environmental sciences. But while the goals proposed for the National Institute for the Environment were universally applauded, Congress was averse to adding a new agency to the federal bureaucracy. Recently, a compromise plan has been proposed that could expand the science base without having to create a new agency. On 29 July 1999, the National Science Board approved an interim report recommending an expanded program of environmental research and research planning, education, and scientific assessment with a funding target of an additional $1 billion over five years. The report stresses the importance of environmental research in formulating environmental protection programs and contains 12 recommendations intended to enhance and complement existing research activities in environmental sciences and engineering. If the National Science Foundation implements the recommendations in the report and if Congress appropriates funds for that purpose, the need for additional funding for new science activities identified by the CNIE should be satisfied. (+info)
Rhizobium-legume symbiosis and nitrogen fixation under severe conditions and in an arid climate.
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Biological N(2) fixation represents the major source of N input in agricultural soils including those in arid regions. The major N(2)-fixing systems are the symbiotic systems, which can play a significant role in improving the fertility and productivity of low-N soils. The Rhizobium-legume symbioses have received most attention and have been examined extensively. The behavior of some N(2)-fixing systems under severe environmental conditions such as salt stress, drought stress, acidity, alkalinity, nutrient deficiency, fertilizers, heavy metals, and pesticides is reviewed. These major stress factors suppress the growth and symbiotic characteristics of most rhizobia; however, several strains, distributed among various species of rhizobia, are tolerant to stress effects. Some strains of rhizobia form effective (N(2)-fixing) symbioses with their host legumes under salt, heat, and acid stresses, and can sometimes do so under the effect of heavy metals. Reclamation and improvement of the fertility of arid lands by application of organic (manure and sewage sludge) and inorganic (synthetic) fertilizers are expensive and can be a source of pollution. The Rhizobium-legume (herb or tree) symbiosis is suggested to be the ideal solution to the improvement of soil fertility and the rehabilitation of arid lands and is an important direction for future research. (+info)
Method to detect genotype-environment interactions for quantitative trait loci in association studies.
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Khoury et al. (Am J Hum Genet 1988;42:89-95 and Am J Epidemiol 1993;137:1241-50) presented an epidemiologic approach to examine genotype-environment interaction in situations where the disease is either present or absent. In this article, the author extends the approach of Khoury et al. to quantitative outcome variables. This extension is relevant for diseases that are extremes on a continuum or when continuous risk factors are studied. To account for a possible admixture of subgroups in the sample, tests for genotype-environment interaction are discussed for designs with parents as controls as well as without parents as controls. Assuming two environmental conditions, the author demonstrates how the power of these tests can be calculated and used to estimate the sample sizes needed to detect genotype-environment interaction in a variety of conditions. In addition, he analyzes simulated data to demonstrate the detection of different mechanisms of genotype-environment interaction and to study the effectiveness of this approach to identify the correct mechanism. Finally, extensions to multiple environmental conditions and designs with multiple subjects per family are discussed. (+info)
Effect of enriched environment rearing on impairments in cortical excitability and plasticity after prenatal alcohol exposure.
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The daily ingestion of alcohol by pregnant mammals exposes the fetal brain to varying levels of alcohol through the placental circulation. Here we focus on the lingering impact on cortical function of 6.5% alcohol administered in a liquid diet to pregnant rats throughout gestation, followed by 3 alcohol-free months before brain function was analyzed in the offspring. Both spontaneous activity of the neurons in the barrel cortex and the level of response to test stimuli applied to the whiskers remained reduced by >75% after alcohol exposure. Whisker pairing, a type of cortical plasticity induced by trimming all but two whiskers in adult rats, occurred in <1 d in controls, but required 14 d to reach significance after alcohol exposure. These long-term neuronal deficits are present in all layers of cortex and affect neurons with both fast and slow action potentials. Plasticity is first seen in the total sample of neurons at 14 d; however, by 7 d, neurons in layer II/III already show plasticity, with no change in layer IV neurons, and a reverse shift occurs toward the inactive whisker in layer V neurons. Analysis of NMDA receptor subunits shows a persistent, approximately 30-50% reduction of NR1, NR2A, and NR2B subunits at postnatal day 90 in the barrel field cortex. Exposing the prenatal alcohol-exposed rats to enriched rearing conditions significantly improves all measured cortical functions but does not restore normal values. The results predict that combinations of interventions will be necessary to completely restore cortical function after exposure of the fetal brain to alcohol. (+info)
Discontinuous gas exchange cycles in aphodius fossor (Scarabaeidae): a test of hypotheses concerning origins and mechanisms.
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It has been suggested that discontinuous gas exchange cycles (DGCs) in insects evolved in response to hypoxic and/or hypercapnic environments, and that the flutter phase of the DGC is predominantly diffusive. We tested these hypotheses by examining the rate of CO(2) release at a declining series of oxygen partial pressures in adults of Aphodius fossor, an inhabitant of moist to wet dung pats that can have low oxygen and high carbon dioxide concentrations. This mesic species showed a pronounced DGC, but progressively abandoned it when exposed to declining oxygen concentrations. That is, in response to a reduction in oxygen concentration, the closed phase declined in length and eventually disappeared, the flutter phase declined in length, while the flutter phase rate of CO(2) release increased, the open phase peak rate of CO(2) release declined and DGC frequency increased. Nonetheless, overall CO(2) release rate remained virtually unchanged. Thus, it appears that the flutter phase in A. fossor has a significant convective component. In addition, in response to declining oxygen concentrations, the spiracles are opened for prolonged periods to increase gas exchange. This behaviour is unlikely to incur a significant water loss penalty in the moist habitats in which A. fossor lives. Given the switch to continuous ventilation by A. fossor under hypoxic conditions, we conclude that current adaptive explanations for the DGC are inadequate. Rather, the DGC may be the default state of a system regulated by two interacting feedback loops when demands are absent. (+info)
Environmentally constrained mutation and adaptive evolution in Salmonella.
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The relationship between environment and mutation is complex [1]. Claims of Lamarkian mutation [2] have proved unfounded [3-5]; it is apparent, however, that the external environment can influence the generation of heritable variation, through either direct effects on DNA sequence [6] or DNA maintenance and copying mechanisms [7-10], or as a consequence of evolutionary processes [11-16]. The spectrum of mutational events subject to environmental influence is unknown [6] and precisely how environmental signals modulate mutation is unclear. Evidence from bacteria suggests that a transient recombination-dependent hypermutational state can be induced by starvation [5]. It is also apparent that changes in the mutability of specific loci can be influenced by alterations in DNA topology [10,17]. Here we describe a remarkable instance of adaptive evolution in Salmonella which is caused by a mutation that occurs in intermediate-strength osmotic environments. We show that the mutation is not 'directed' and describe its genetic basis. We also present compelling evidence in support of the hypothesis that the mutational event is constrained by signals transmitted from the external environment via changes in the activity of DNA gyrase. (+info)