Dipylidium caninum infection. (73/109)

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Perch and its parasites as heavy metal biomonitors in a freshwater environment: the case study of the Ruzin water reservoir, Slovakia. (74/109)

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Revision of Gangesia (Cestoda: Proteocephalidea) in the Indomalayan region: morphology, molecules and surface ultrastructure. (75/109)

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Determination of the electrophoretic pattern of somatic and excretory-secretory proteins of Ligula intestinalis parasite in spirlin (Alburnoides bipunctatus). (76/109)

Ligula intestinalis parasite is a cestodes that causes remarkable damages to fish. It is also of prime importance in economic and hygienic aspects. SDS-PAGE and western blotting are the methods that can be used to determine the electerophoretic pattern of somatic and excretory-secretory proteins of parasites. In this study, after obtaining the plerocercoidal stage of this parasite from the spirlin (Alburnoides bipunctatus), its somatic proteins were prepared using ultrasonicae, and excretory-secretory proteins were prepared using the PBS solution. After protein assay, which included using the Bradford method and then SDS-PAGE on these two antigenic solutions, 5 protein bands of 26, 33, 38, 58, 70kDa in somatic antigens, and 7 bands of 25, 28, 33, 43, 49, 60, 70kDa in excretory-secretory antigens were observed. After western blotting on both antigens and adding the primary antibody (the sera of infected fish) and then the secondary antibody (Rabbit Anti-fish Polyclonal Antibody Conjugated from Abnova Corporation) no band was seen in excretory-secretory antigen. And only in the 55kDa band of somatic antigen, a positive response, in comparison of fish positive serum was observed.  (+info)

Natural parasite infection affects the tolerance but not the response to a simulated secondary parasite infection. (77/109)

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The parasitic communities of the rock pigeon Columba livia from Iraq: component and importance. (78/109)

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Tapeworm eggs in a 270 million-year-old shark coprolite. (79/109)

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Hybridization between two cestode species and its consequences for intermediate host range. (80/109)

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