Characterization of a new beta(1-3)-glucan branching activity of Aspergillus fumigatus. (49/122)

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Yeast surface display of trifunctional minicellulosomes for simultaneous saccharification and fermentation of cellulose to ethanol. (50/122)

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Development and application of a PCR-targeted gene disruption method for studying CelR function in Thermobifida fusca. (51/122)

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Large-scale analyses of glycosylation in cellulases. (52/122)

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Molecular insight into lignocellulose digestion by a marine isopod in the absence of gut microbes. (53/122)

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Interplay between Clostridium thermocellum family 48 and family 9 cellulases in cellulosomal versus noncellulosomal states. (54/122)

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Cocktail delta-integration: a novel method to construct cellulolytic enzyme expression ratio-optimized yeast strains. (55/122)

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Complete saccharification of cellulose at high temperature using endocellulase and beta-glucosidase from Pyrococcus sp. (56/122)

We investigated a potential for glucose production from cellulose material using two kinds of hyperthermophilic enzymes, endo-cellulase (EG) and beta-glucosidase (BGL). Two BGLs from hyperthermophile Pyrococcus furiosus and mesophile Aspergillus aculeatus were compared for complete hydrolysis of cellulose with P. horikoshii endo-cellulase (EGPh). The combination reactions by each BGL enzyme and EGPh could produce only glucose without the other oligosaccharides from phosphoric acid swollen Avicel (PSA). The combination of the both hyperthermophilic cellulases, BGLPf and EGPh, will be adaptable to high efficient system to produce glucose at high temperature.  (+info)