Estrogenic and anti-estrogenic activities of the Thai traditional herb, Butea superba Roxb.
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This study evaluated the estrogenic and antiestrogenic activities of native and in vitro hepatic metabolized tuberous extracts of wild Butea superba collected from 23 out of the 76 provinces in Thailand by yeast estrogen screening (YES). The YES screen used consisted of the human estrogen receptors hERalpha and hERbeta and the human transcriptional intermediary factor 2 or human steroid receptor coactivator 1, respectively, together with the beta-galactosidase expression cassette as the reporter. The relative potency, effectiveness and relative inductive efficiency were evaluated by determining the beta-galactosidase activity (EC(50)) of each tuberous extract in relation to that induced by 17beta-estradiol. Six pure compounds isolated from B. superba were tested in parallel and exhibited a maximum relative potency compared to 17beta-estradiol of 15.5% and 5.27% in the respective hERalpha and hERbeta assays. Eighteen and seventeen plant extracts were respectively found to interact with the hERalpha and hERbeta receptors in the YES assays with higher relative potency and relative inductive efficiency with hERbeta than with hERalpha. The selected plant extracts tested exhibited antiestrogenic activity. Coincubation with the rat liver S9 mixture also elevated the estrogenic potency of these plant extracts. (+info)
Crystallization and preliminary X-ray diffraction analysis of a galactose-specific lectin from the seeds of Butea monosperma.
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