A novel method for purifying bluetongue virus with high purity by co-immunoprecipitation with agarose protein A. (65/204)

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Colostral transmission of bluetongue virus nucleic acid among newborn dairy calves in California. (66/204)

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Intragenic recombination as a mechanism of genetic diversity in bluetongue virus. (67/204)

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Experimental oral infection of bluetongue virus serotype 8 in type I interferon receptor-deficient mice. (68/204)

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Anatomy of bluetongue virus serotype 8 epizootic wave, France, 2007-2008. (69/204)

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A broad assessment of factors determining Culicoides imicola abundance: modelling the present and forecasting its future in climate change scenarios. (70/204)

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Expression and characterization of bluetongue virus serotype 21 VP7 antigen: C-terminal truncated protein has significantly reduced antigenicity. (71/204)

In the present study, group-specific antigen VP7 of bluetongue virus (BTV) serotype 21 isolated from cattle in Tochigi prefecture in Japan in 1994 was characterized by sequencing and expression. Gene was amplified from cDNA synthesized on viral dsRNA using reverse-transcriptase-PCR. Nucleotide sequence of this isolate showed high similarity with other published BTV VP7 sequences. Full-length and C-terminal truncated forms of VP7 were expressed in insect cells by a baculovirus gene expression system under control of the viral polyhedrin promoter. Expression of full-length recombinant VP7 was confirmed by immunoprecipitation with VP7 specific monoclonal antibody (8A3B.6, ATCC). Recombinant proteins expressed with or without 6x His-tag showed good expression levels in TN5 cells and reacted well with the monoclonal antibody in the indirect ELISA. However C-terminal truncated VP7 with His-tag failed to react with this monoclonal antibody, while poor antigenicity was evident when it was reacted with infected bovine serum. Reduced antigenicity of the latter suggested that C-terminal truncation affects 8A3B.6 epitope construction probably via inhibition of VP7 trimer structure formation.  (+info)

Possible windborne spread of bluetongue to Portugal, June-July 1956. (72/204)

The possible sources for the epidemic of bluetongue in Portugal at the beginning of July 1956 were examined. Introduction through authorized importation of domestic or wild ruminants was not feasible, since no cattle, sheep or goats were imported and the wild ruminants were confined to Lisbon Zoo, which was too far from the initial outbreaks. Weather maps were examined to see if the wind could have carried infected Culicoides midges from North Africa. On 21 June 1956 infected midges in Morocco could have been taken offshore by southeast winds and then carried by south winds unusual at that time of year to the south coast of Portugal. The 200-300 km sea crossing would have taken some 10 h and been by day when air temperatures near the sea surface were about 18-20 C. Bluetongue had not been reported at that time in Moroccco, and the possibility of the presence of the virus in moroccan animals without clinical signs is discussed.  (+info)