Early biofilm formation and the effects of antimicrobial agents on orthodontic bonding materials in a parallel plate flow chamber.
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Decalcification is a commonly recognized complication of orthodontic treatment with fixed appliances. A technology, based on a parallel plate flow chamber, was developed to investigate early biofilm formation of a strain of Streptococcus sanguis on the surface of four orthodontic bonding materials: glass ionomer cement (Ketac Cem), resin-modified glass ionomer cement (Fuji Ortho LC), chemically-cured composite resin (Concise) and light-cured composite resin (Transbond XT). S. sanguis was used as it is one of the primary colonizers of dental hard surfaces. Artificial saliva was supplied as a source of nutrients for the biofilms. The effects of two commercially available mouthrinses (i.e. a fluoride containing rinse and chlorhexidine) were evaluated. Initial colonization of the bacterium was assessed after 6 hours of growth by the percentage surface coverage (PSC) of the biofilm on the disc surfaces. There were statistically significant differences in bacterial accumulation between different bonding materials (P < 0.05), Concise being the least colonized and Transbond XT being the most colonized by S. sanguis biofilms. All materials pre-treated with 0.05 per cent sodium fluoride mouthrinse showed more than 50 per cent reduction in biofilm formation. The 0.2 per cent chlorhexidine gluconate mouthrinse caused significant reduction of biofilm formation on all materials except Ketac Cem. This in vitro study showed that the use of a chemically-cured composite resin (Concise) reduced early S. sanguis biofilm formation. Also, fluoride had a greater effect in reducing the PSC by S. sanguis biofilms than chlorhexidine. Rinsing with 0.05 per cent sodium fluoride prior to placement of orthodontic appliances is effective in reducing early biofilm formation. (+info)
Effect of enamel preparations on fracture resistance of composite resin buildup of fractures involving dentine in anterior bovine teeth: an in vitro study.
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Sixty bovine teeth with simulated mesio-incisal angle fracture were randomly and equally divided in one untreated (control) group and three experimental groups (Bevel, Chamfer and newly introduced Stair-step Chamfer preparation group) to evaluate the effect of enamel preparations on the fracture resistance of composite resin. Post restoration, fracture mechanics approach was used to quantify the failure of composite resins in testing the samples in Instron testing machine. Mean peak failure load (Newton) of composite amongst experimental groups was observed in the order; Chamfer (326.09 +/- 72.73), Stair-step chamfer (315.21 +/- 81.77) and Bevel (253.83 +/- 67.38). Results of the One-Way ANOVA revealed significant difference in the mean peak failure load values of the four different groups. (P<0.001) Scheffe's Post-Hoc comparison test (Subset for alpha = 0.05) revealed that there was no significant difference in the mean peak failure load values of the bevel, stair-step chamfer and chamfer preparation when considered together, but the mean peak values of control group (605.22 +/- 48.96) were observed significantly higher. Failure mode evaluation revealed, majority of failures occurred as cohesive and mixed type for all the experimental groups. Adhesive type failure was observed maximum (33%) in the bevel group. Stair-step chamfer preparation showed greatest potential for application and use as it no only demonstrated comparable values to Chamfer preparation ['t' value (0.39) (P > 0.05)] but also involved sacrificing less amount of tooth structure adjacent to fractured edge. (+info)
Characterization of photopolymerization of dentin adhesives as a function of light source and irradiance.
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Manufacturers have attempted to address the limitations associated with dentin bonding by eliminating as many steps as possible in the bonding protocol. Theoretically, this approach increases the efficiency of the procedure and reduces technique sensitivity. These trends are reflected in the introduction of all-in one, single-step adhesive systems; the increased concentration of acidic resin monomers in these systems allows for simultaneous etching and priming of the prepared dentin surface. Ideally, the degree of monomer conversion would be high enough that the acidic reaction would be self-limiting. The purpose of this study was to investigate the effect of light irradiance and source on the photopolymerization of three commercial dental adhesives by monitoring the double bond conversion as a function of time during and after irradiation. The photopolymerization curing efficiency of the commercial adhesives investigated in this study varied as a function of light source and distance. The use of LED performed better than the halogen light in terms of polymerization rate and degree of conversion for the commercial single-step, sixth generation adhesive, Adper Prompt. In contrast, polymerization of commercial single-bottle, fifth generation adhesive, Single Bond and One-Up Bond F, was mainly a function of exposure time, irrespective of the two light units or intensities. (+info)
Effects of a glass-ionomer cement on the remineralization of occlusal caries--an in situ study.
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This work evaluated the remineralization of demineralized enamel of pits and fissures of human third molars sealed with a glass ionomer cement (Fuji IX, GC Corporation--Japan) or with a Bis-GMA sealant (Delton--Dentsply). Ten volunteers participated in this in situ study that consisted of two thirty-day periods using intra-oral devices, with a weeks interval in between. Four experimental treatment procedures and one control were randomly assigned to the volunteers specimens: Group I, no treatment, control; Group II, artificial caries process; Group III, same treatment as Group II, but sealed with Delton (Dentsply); Group IV, same treatment as Group II, but sealed with Fuji IX (GC Corporation--Japan); Group V, same treatment as Group II and no sealing. Groups I and II were not submitted to the oral environment and served as controls. After a period of 30 days in the oral environment, the specimens were removed from the devices, embedded in acrylic resin, ground flat and polished. Then, Knoop hardness tests were performed, with a 25 g static load applied for 15 seconds. The measurements were made from the base of the fissure up to an opening of 600 microm, pre-established between the inclines of the cusps. Three indentations were then made, located at 25, 75, and 125 microm in depth from the outer enamel margin and 100 microm apart from each other (Micromet 2003). The Brieger F and Bonferronis tests were applied to the measurements. It was concluded that sealing with the glass ionomer cement Fuji IX was capable of making the enamel of pits and fissures more resistant by increasing the value of Knoop hardness. (+info)
Degradation of methacrylate monomers in human saliva.
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This study assessed the effect of the molecular structure of newly synthesized methacrylate monomers on their chemical stability in human saliva, whereby these monomers can be used as dental composite resins. Six model monomethacrylates and two urethane-modified BisGMA monomers were added to human saliva, and their change in concentration after 24, 48, and 72 hours were measured by high-performance liquid chromatography. Degradation of the six model monomethacrylate monomers was found to be influenced by the molecular structure, such as steric hindrance and presence of urethane bond in chemical backbone. Based on the degradation test results of these six monomers, urethane-modified BisGMA derivatives--in which the hydroxyl groups in original BisGMA monomer were substituted with alkyl isocyanate--were synthesized and subjected to degradation test. The urethane-modified BisGMA monomers showed a particular resistance to salivary hydrolysis. Results of this study thus suggested that urethane groups should be considered when designing new monomers for dental composite systems as they demonstrated improved resistance to hydrolysis. (+info)
Development of dental composite resin utilizing low-shrinking and low-viscous monomers.
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To lower the viscosity of composite resins, experimental composite resins were produced using low-viscosity monomer mixtures of newly developed polyfunctional acrylates, and the mechanical and physical properties of the hardened composites were investigated. Mechanical (i.e., compressive, diametral tensile, and bending) strength of a polymer obtained from one new monomer mixture without fillers was similar to that of a bis-GMA/TEGDMA (2/1 weight ratio) based polymer. As for the hardened composites, the mechanical strength of composites produced using the new monomer mixtures showed a different tendency from that of bis-GMA based composites. Further, even the viscosity of composite pastes with high filler content was markedly lower than that of bis-GMA based composites. In terms of setting shrinkage, the composites consisting of new monomer mixtures exhibited significantly smaller shrinkage than the bis-GMA based composites, and decreased with increase in filler content. (+info)
Chemical profile of adhesive/caries-affected dentin interfaces using Raman microspectroscopy.
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In clinical practice, dentists must frequently bond adhesives to caries-affected dentin substrates, but the bond that characteristically forms with these substrates does not provide the durability necessary for long-term clinical function. The purpose of this study was to characterize and compare the interfacial chemistry of adhesive with caries-affected and noncarious dentin using micro-Raman spectroscopy. The results indicated that the differences in the Raman spectra between noncarious and caries-affected dentin could not be accounted for by simple decreased mineralization. Both the structure of collagen and mineral in the caries-affected dentin has been altered by the caries process. The differences in structure and composition not only interfered with acid-etching process but also subsequent resin monomer penetration. It was shown that the interface between the adhesive and caries-affected dentin was wider and more complicated than that of the adhesive and noncarious dentin. As a result of adhesive phase separation, a structurally integrated hybrid layer did not form at the interface with either caries-affected or noncarious dentin. Using chemical imaging techniques, this study provides the direct evidence of adhesive phase separation at the interface with caries-affected dentin. Although our group previously reported adhesive phase separation at the interface with noncarious dentin, the chemistry of caries-affected dentin leads to greater variability and a more highly irregular composition along the length and breadth of the interface. (+info)
Morphological characterization of the tooth/adhesive interface.
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The purpose of this study was to assess the morphological characteristics of the tooth/adhesive interface using different adhesive systems in MOD restorations under scanning electron microscopy (SEM). The tested hypothesis was that the morphology of the bonding interface would vary in different areas of MOD restorations for the three adhesive systems. MOD cavities were prepared in 12 sound extracted human third molars and restored with Filtek Z250 composite resin and one of the following adhesive systems: Experimental ABF (n=4), Clearfil SE Bond (n=4) self-etching primers and Single Bond etch-and-rinse adhesive system (n=4). After 24-h storage in distilled water at 37 degrees C, teeth were sectioned and prepared for SEM. The interfacial morphology varied depending on the adhesive system and also on the evaluated area. The null hypothesis was accepted because the morphology of the tooth/adhesive interface reflected the characteristics of both the dental substrate and the adhesive systems. (+info)