Some observations concerning microsclerotia and spore production of Mycoleptodiscus terrestris in culture.
(9/30)
Some interesting growth characteristics of Mycoleptodiscus terrestris were discovered while evaluating the fungus as a potential bioherbicide for management of the submersed macrophyte, Hydrilla verticillata. Microsclerotia were produced readily in a liquid culture medium containing a basal salts solution supplemented with corn steep liquor powder and glucose. Dried microsclerotia were capable of germinating hyphally within 24 h and sporogenically within 72 h. Initiation of spore production was not dependent on the presence of host plant material or light. (+info)
Pelagicoccus croceus sp. nov., a novel marine member of the family Puniceicoccaceae within the phylum 'Verrucomicrobia' isolated from seagrass.
(10/30)
An obligately aerobic, spherical, non-motile, pale-yellow pigmented bacterium was isolated from a piece of leaf of seagrass, Enhalus acoroides (L.f.) Royle, grown in Okinawa, Japan and was subjected to a polyphasic taxonomic study. Phylogenetic analyses based on 16S rRNA gene sequences revealed that the novel isolate N5FB36-5(T) shared approximately 96-98 % sequence similarity with the species of the genus Pelagicoccus of the family Puniceicoccaceae within the phylum 'Verrucomicrobia'. The DNA-DNA relatedness values of strain N5FB36-5(T) with Pelagicoccus mobilis 02PA-Ca-133(T) and Pelagicoccus albus YM14-201(T) were below 70 %, which is accepted as the phylogenetic definition of a novel species. beta-Lactam antibiotic susceptibility test and amino acid analysis of the cell wall hydrolysates indicated the absence of muramic acid and diaminopimelic acid in the cell walls, which suggested that this strain lacks an ordinary Gram-negative type of peptidoglycan in the cell wall. The DNA G+C content of strain N5FB36-5(T) was 51.6 mol%; MK-7 was the major menaquinone; and the presence of C(16 : 0), C(16 : 1)omega7c and anteiso-C(15 : 0) as the major cellular fatty acids supported the identification of the novel isolate as a member of the genus Pelagicoccus. On the basis of polyphasic taxonomic data, it was concluded that this strain should be classified as a novel species of the genus Pelagicoccus, for which the name Pelagicoccus croceus sp. nov. is proposed. The type strain is N5FB36-5(T) (=MBIC08283(T)=KCTC [corrected] 12903(T)). (+info)
Liquid culture and growth quantification of the seagrass pathogen, Labyrinthula spp.
(11/30)
Symptoms characteristic of wasting disease, thought to result from infection by protozoan pathogens (i.e. Labyrinthula spp.), are a common phenomenon affecting seagrass species worldwide. However relatively little is known about factors that control the survival and success of Labyrinthula in part due to the difficulty associated with quantifying the growth of this organism. Here we describe a simple and inexpensive method for measuring growth of Labyrinthula in liquid culture that takes into consideration both cell density and areal spread. The technique allows for examination of the effects of both abiotic and biotic factors on the growth of Labyrinthula apart from its seagrass host, separating the effects of environmental condition on the host from their effects on the pathogen. (+info)
Flow enhances photosynthesis in marine benthic autotrophs by increasing the efflux of oxygen from the organism to the water.
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Antinociception produced by Thalassia testudinum extract BM-21 is mediated by the inhibition of acid sensing ionic channels by the phenolic compound thalassiolin B.
(14/30)