Identification of the forensically important sarcophagid flies Boerttcherisca peregrina, Parasarcophaga albiceps and Parasarcophaga dux (Diptera: Sarcophagidae) based on COII gene in China.
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Accurate species identication is a crucial step in forensic entomology, as the insect collected on a corpse can provide useful information for estimation of postmortem interval (PMI). The utility of the forensically important Sarcophagidae (Diptera) for crime scene investigation has been severely restricted, as morphological identification is difficult, especially the identification of females and larvae. In this study, a method for using mitochondrial DNA (mtDNA) sequence data and phylogenetic analysis was performed to distinguish the three Chinese sarcophagid species: Boerttcherisca peregrina (Robineau-Desvoidy, 1830) Parasarcophaga albiceps (Meigen, 1826) and Parasarcophaga dux (Thompson, 1869) (Diptera: Sarcophagidae). DNA was extracted and analyzed by a 189 bp fragment of cytochrome coxidase subunit II (COII) gene. The monophyletic branches of the phylogenetic tree reveal that this marker is suitable for discrimination between these species, providing high support for separation on congeneric species. Therefore, the molecular method applied to the sarcophagid species identification is feasible. (+info)
Colonization of Grande Comore Island by a lineage of Rhipicephalus appendiculatus ticks.
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Entomological patterns in the human African trypanosomiasis focus of Komo Mondah, Gabon.
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BACKGROUND: The incidence of sleeping sickness is still considerable in the Komo Mondah focus, in spite of case-detection strategy. A combined strategy that associated both mass screening and vector control is effective for the control of the disease. In the perspective of a targeted vector control in main transmission sites, we have carried out an entomological survey in the epicentre of the focus. OBJECTIVES: To determine tsetse flies distribution, human-fly contact point and eventually risk factors for acquisition of the disease. METHODS: "Vavoua" traps were set for Glossina in four biotopes selected after an interview of HAT patients concerning their working places. Tsetse were captured and dissected. DNA from organs was analysed by PCR for trypanosome infections. The origin of blood meals was determined by ELISA. RESULTS: The focus is infested by three species of Glossina: G. palpalis palpalis (1149: 91.85%) found in all biotopes; G. fuscipes fuscipes (85: 6.79%) and G. caliginea (17: 1.36%) found in water spots and landing stages. They are infected by three subgenera of trypanosomes and only G. palpalis palpalis is infected by human trypanosomes. G. fuscipes fuscipes is infected by T. brucei sl and G. caliginea is not infected. Flies are absent at the periphery of houses except in one village. Only 29.20% of blood meals were from humans. Landing stages built in swamp mangrove are presenting the higher index of epidemiological risk and populations are exposed to the disease when they go to the area for taking their fishing boats. CONCLUSION: Swamp mangrove would be targeted in priority during a vector control campaign. (+info)
Monitoring mosquitoes in urban Dar es Salaam: evaluation of resting boxes, window exit traps, CDC light traps, Ifakara tent traps and human landing catches.
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A comparison of the pitfall trap, Winkler extractor and Berlese funnel for sampling ground-dwelling arthropods in tropical montane cloud forests.
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Role of the repartition of wetland breeding sites on the spatial distribution of Anopheles and Culex, human disease vectors in southern France.
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