Amino Acid SubstitutionAmino Acid SequenceMolecular Sequence DataAmino AcidsSequence AlignmentSequence Homology, Amino AcidMutationBase SequenceMutagenesis, Site-DirectedSequence Analysis, ProteinCloning, MolecularPoint MutationStructure-Activity RelationshipModels, MolecularBinding SitesEscherichia coliProtein ConformationProteinsProtein Structure, TertiaryMutation, MissenseSequence Analysis, DNAKineticsRecombinant ProteinsPhylogenyCell LineDNA Mutational AnalysisEvolution, MolecularProtein BindingAlgorithmsMutagenesisDatabases, ProteinGenetic VariationBacterial ProteinsCodonAmino Acid MotifsSubstrate SpecificityPlasmidsProtein Structure, SecondarySequence Homology, Nucleic AcidConserved SequencePeptide FragmentsPolymerase Chain ReactionDNADNA PrimersComputational BiologySoftwareDNA, ComplementaryAlaninePhenotypeAllelesRecombinant Fusion ProteinsPeptidesAmino Acids, EssentialSpecies SpecificityRestriction MappingGlycineLeucineViral ProteinsAmino Acid Transport SystemsAspartic AcidGenes, BacterialProlineTransfectionEnzyme StabilityLysineArginineCysteineSequence DeletionCricetinaeSaccharomyces cerevisiaeIsoleucineMutant ProteinsValineEpitopesElectrophoresis, Polyacrylamide GelCatalysisDNA, BacterialPolymorphism, GeneticCarrier ProteinsEscherichia coli ProteinsSelection, GeneticExonsViral Envelope ProteinsGenesDNA-Binding ProteinsVirus ReplicationSerineCOS CellsTemperatureRNA, MessengerCercopithecus aethiopsProtein EngineeringPhenylalanineTranscription, GeneticAsparagineThreonineCattleGlutamineGenotypeModels, Genetic