Oceanisphaera litoralis gen. nov., sp. nov., a novel halophilic bacterium from marine bottom sediments.
(1/11)
A polyphasic taxonomic study was performed to characterize a new bacterial isolate, designated KMM 3654(T), from a marine bottom sand sample. The strain was Gram-negative, encapsulated, aerobic, moderately halophilic and grew between 0.5 and 10 % NaCl and at 4-42 degrees C. Its DNA G+C content was 56.4 mol%. Isolate KMM 3654(T) was phylogenetically closely related to members of the genus Oceanimonas, showing 96.7 and 95.6 % 16S rRNA gene sequence similarity to Oceanimonas doudoroffii DSM 7028(T) and Oceanimonas baumannii ATCC 700832(T), respectively. Strain KMM 3654(T) shared some physiological and chemotaxonomic properties with these two Oceanimonas species, but differed from them in morphology, growth at 4 degrees C, urease activity, weak phenol degradation and utilization of phenylacetate. On the basis of phenotypic and phylogenetic evidence, Oceanisphaera litoralis gen. nov., sp. nov. is proposed, with the type strain KMM 3654(T) (=DSM 15406(T)). (+info)
Oceanisphaera donghaensis sp. nov., a halophilic bacterium from the East Sea, Korea.
(2/11)
A taxonomic study was carried out on two isolates, strains BL1(T) and BL11, from marine sediment collected from the East Sea, Korea. Comparative 16S rRNA gene sequence studies showed that these isolates clearly affiliated with the Gammaproteobacteria. BL1T and BL11 were most closely related to Oceanisphaera litoralis KMM 3654T (97.6 and 97.7 % 16S rRNA gene sequence similarity, respectively). The level of 16S rRNA gene sequence similarity between strains BL1T and BL11 was 99.7 %. The two isolates were Gram-negative, aerobic, moderately halophilic, and grew in 0.5-8.0 % NaCl and at 4-42 degrees C. Strains BL1T and BL11 shared some physiological and biochemical properties with O. litoralis KMM 3654T, although they differed in that BL1T and BL11 were able to utilize ethanol, proline and alanine. The G+C contents of the genomic DNA of strains BL1T and BL11 were 56.6 and 57.1 mol%, respectively. Both strains possessed C(16 : 1)omega7c and/or iso-C(15 : 0) 2-OH, C(16 : 0) and C(18 : 1)omega7c as the major fatty acids. DNA-DNA relatedness data indicated that strains BL1T and BL11 represent a genomic species that is separate from O. litoralis KMM 3654T. On the basis of polyphasic evidence, it is proposed that strain BL1T (=KCTC 12522T = DSM 17589T) represents the type strain of a novel species, Oceanisphaera donghaensis sp. nov. (+info)
Zobellella denitrificans gen. nov., sp. nov. and Zobellella taiwanensis sp. nov., denitrifying bacteria capable of fermentative metabolism.
(3/11)
Two denitrifying strains of heterotrophic, facultatively anaerobic bacteria, designated ZD1(T) and ZT1(T), were isolated from sediment samples collected from mangrove ecosystems in Taiwan. The isolates were Gram-negative. Cells grown in broth cultures were straight rods that were motile by means of a single polar flagellum. The isolates grew optimally in 1-3 % NaCl, but NaCl was not an absolute requirement for growth; only strain ZT1(T) grew in 13-14 % NaCl. Both isolates grew between 10 and 45 degrees C, with optimum growth at 30-35 degrees C. They were capable of anaerobic growth by denitrifying metabolism using nitrate or nitrous oxide as terminal electron acceptors or, alternatively, by fermenting glucose, sucrose or mannitol as substrates. C(18 : 1)omega7c was the most abundant fatty acid (32.6-35.7 %). The other major fatty acids included C(16 : 1)omega7c (27.5-29.4 %) and C(16 : 0) (20.1-22.0 %). The two isolates had 16S rRNA gene sequence similarity of 96.8 % and shared 94.1-96.8 % sequence similarity with the most closely related species, Oceanimonas doudoroffii, Oceanimonas baumannii, Oceanimonas smirnovii and Oceanisphaera litoralis. They could be distinguished from these species in that they were capable of fermentative metabolism, had relatively high DNA G+C contents (62.0-64.0 mol%) and contained C(18 : 1)omega7c instead of C(16 : 1)omega7c as the most abundant fatty acid. Characterization data accumulated in this study revealed that the two denitrifying isolates could be classified as representatives of two novel species in a new genus, Zobellella gen. nov., with Zobellella denitrificans sp. nov. (type strain ZD1(T) = BCRC 17493(T) = JCM 13380(T)) as the type species and Zobellella taiwanensis sp. nov. (type strain ZT1(T) = BCRC 17494(T) = JCM 13381(T)) as a second species. (+info)
Poly(3-hydroxybutyrate) production from glycerol by Zobellella denitrificans MW1 via high-cell-density fed-batch fermentation and simplified solvent extraction.
(4/11)
Oceanisphaera ostreae sp. nov., isolated from seawater of an oyster farm, and emended description of the genus Oceanisphaera Romanenko et al. 2003.
(7/11)