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Essential vitamin. (Balance diet)
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A typical sgRNA contains 98 nucleotides (including a 20-nt target sequence) and functions as a guide in the Cas9/sgRNA nuclease complex. To improve Cas9 expression in plants, most modified Cas9 genes for genome editing have also been optimized with usage bias codons.

https://www.creative-biogene.com/crispr-cas9/products/knockout-mice-659.html
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