The virus bank, which consists of master viral bank and working viral bank, is an important part of manufacturing a biological product, such as vaccine, recombinant protein. A master viral bank is the base of reproducible testing and production of recombinant virus for products, while working viral bank is a tool to amplify and produce the products. To ensure the characterization of virus bank is a quality assurance of subsequent process. Thus, characterization of virus bank is vital to assure the safety of virus-derived biopharmaceutical products. The objective of virus bank characterization is to confirm identity, purity, genetic stability.