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CRISPR/Cas9 system used for genome engineering in molecular biology is composed of two components: sgRNA which is a combine of original trRNA and crRNA, and Cas9 endonuclease. By delivering Cas9 and sgRNA into a cell, Cas9 is targeted to a given locus based on sequence complementary between sgRNA and cell genome, and further induces a double strand break (DSB). 

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