Cysteine dioxygenase. Medical search. Frequent questions (1)

Cysteine dioxygenase (CDO) is a non-heme iron enzyme that catalyzes the conversion of L-cysteine to cysteine sulfinic acid (cysteine sulfinate). (wikipedia.org)
It oxidizes cysteine to the corresponding sulfinic acid by activation of dioxygen, although the exact mechanism of the reaction is still unclear. (wikipedia.org)
CDO oxidizes to cysteine sulfinic acid (which exists predominantly in the anionic sulfinate form in vivo). (wikipedia.org)
Cysteine dioxygenase (CDO) catalyzes the oxidation of cysteine to cysteine sulfinic acid, which is the first major step in cysteine catabolism in mammalian tissues. (umass.edu)

Using pharmacological unmasking microarray, we identified promoter DNA methylation of cysteine dioxygenase 1 (CDO1) gene in human cancer. (nih.gov)

Additionally, after treatment of TE 671 cells with 0.005-0.5 μM 4- n -octylphenol, bis(2-ethylhexyl)phthalate, and diisodecyl phthalate, real-time RT-PCR showed dose-dependent decreases in the steady-state mRNA levels of cysteine dioxygenase type I, sulfite oxidase, and 3′-phosphate 5′-phosphosulfate synthase I. (nih.gov)

Presented here are the results of O2-dependent 2-mercaptoaniline reaction using two different thiol dioxygenase enzymes mouse CDO and 3-mercaptopropionic acid dioxygenase isolated from Azotobacter vinelandii. (genscript.com)
It has been shown that children with food intolerance are slow or null metabolisers of cysteine dioxygenase which is one of the CYP450 enzymes (Breakey 2004), the same enzyme system that cause problems with psychiatric drug intolerance. (fedup.com.au)
Acireductone dioxygenases (ARDs) are enzymes involved in the methionine recycle pathway. (umass.edu)

An enzyme that catalyzes the conversion of L-CYSTEINE to 3-sulfinoalanine (3-sulfino-L-alanine) in the CYSTEINE metabolism and TAURINE and hypotaurine metabolic pathways. (nih.gov)
The existence of two pathways for cysteine synthesis might ensure its participation in the formation of proteins, heavy metal detoxification, and the sulfide-binding function of haemoglobin. (biomedcentral.com)
We elucidated several molecular pathways of sulfate activation, cysteine and cholesterol synthesis, and trehalose metabolism. (biomedcentral.com)
Contrary to the previous analysis, two pathways for cysteine synthesis and the cycloartenol-C-24-methyltransferase gene were identified in animals for the first time. (biomedcentral.com)

Furthermore, research has shown that cysteamine, a structurally similar molecule to cysteine, enhances cysteine oxidation but is not a substrate. (wikipedia.org)
One model postulates that the iron-sensing capacity of the 73d is based on site-specific oxidation of conserved cysteine residues upon direct iron binding [ 11 ]. (biomedcentral.com)

Ten Eleven Translocation-1 knockdown decreased 5-hydroxymethylcytosine formation following hydroquinone exposure as well as the induction of glutamate-cysteine ligase catalytic subunit and 14-3-3σ. (nih.gov)
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Glutamate Cysteine Ligase, Modifier Subunit (GCLM) in Tissue homogenates and other biological fluids. (isogem.org)
Description: A sandwich ELISA kit for detection of Glutamate Cysteine Ligase, Modifier Subunit from Rat in samples from blood, serum, plasma, cell culture fluid and other biological fluids. (isogem.org)

CDO plays an important role in cysteine catabolism, regulating intracellular levels of cysteine and responding changes in cysteine availability. (wikipedia.org)
Has an important role in maintaining the hepatic concentation of intracellular free cysteine within a proper narrow range. (nih.gov)
Using DL-propargylglycine, a particular irreversible CSE inhibitor [15], CSE offers been shown to become important in rat liver organ, kidney and cultured hepatocytes for a satisfactory way to obtain cysteine to synthesize glutathione [11,16,17], a significant intracellular antioxidant that protects cells from oxidative tension. (health-ground.com)
Cysteine can be used for biosynthesis of taurine also, probably the most abundant intracellular free of charge amino acid, which includes numerous biological functions and may become an antioxidant also. (health-ground.com)
However, due to its reactive thiol side chain, elevated levels of intracellular cysteine can be toxic and therefore need to be rapidly eliminated from the cellular milieu. (microbialcell.com)
Overexpressing the high affinity cysteine transporter, YCT1 , enabled yeast cells to rapidly accumulate high levels of intracellular cysteine. (microbialcell.com)

Microarray based gene expression studies revealed the upregulation of arginine/ornithine biosynthesis a few hours after the cysteine overload, and suggest that the non-toxic, non-reactive thiol based metabolic products are eventually utilized for amino acid and polyamine biogenesis, thereby enabling cell growth. (microbialcell.com)

CDO is responsible for the first major step in metabolism of cysteine. (wikipedia.org)
In human cysteine metabolism, L-cysteine is consumed in several ways as shown below. (dadamo.com)

It also has some activity as EC 1.13.11.20 , cysteine dioxygenase, and can perform the reaction of EC 1.14.99.50 , γ-glutamyl hercynylcysteine sulfoxide synthase, albeit with low activity [4]. (enzyme-database.org)

Indoleamine 2,3-dioxygenase (IDO) is a heme-containing dioxygenase that catalyses the first and rate-limiting step in the kynurenine (Kyn) pathway of L-tryptophan (L-Trp)catabolism. (edu.au)

Furthermore, crystal structures show the amino nitrogen and thiolate sulfur of cysteine coordinated to the iron in addition to a single water molecule (see figure). (wikipedia.org)
One proposed mechanism, supported by computational and spectroscopic studies, involves O2 binding cis to a thiolate to form reactive iron (III)-superoxo species (A), which then attacks the bound sulfur of cysteine to form a four-membered ring structure (B). Heterolytic O-O bond cleavage then affords a high-valent iron (IV) oxo intermediate (C), which transfers the second oxygen to sulfur. (wikipedia.org)
The investigation of two non-heme iron dioxygenases with sulfur-containing substrates is reported along with studies of seleno-compounds by gas chromatography with atomic emission detection (GC-AED). (umass.edu)
Moreover, sulfide can serve directly as a sulfur source for cysteine synthesis in L. luymesi . (biomedcentral.com)

In particular, the metabolites 2-aminobutyric acid, cysteine, alanine, and tyrosine all displayed areas under the receiver operating characteristic curve were above 0.9. (biomedcentral.com)
Additional metabolites include benzoquinone, multiple glutathione conjugates, and N -acetyl cysteine conjugates. (nih.gov)

The role of CDO may vary between cell types as it can either be used primarily for taurine or sulfate production or for degradation of cysteine. (wikipedia.org)
CDO is also regulated in adipose tissue, where high cysteine levels cause increased hypotaurine/taurine production. (wikipedia.org)
Of its part as the precursor of such bioactive substances Irrespective, cysteine itself could up-regulate the manifestation of cysteine dioxygenase that mediates taurine creation and down-regulate the manifestation of -glutamylcysteine synthetase, which mediates glutathione creation in cultured rat hepatocytes and intact rats [18,19]. (health-ground.com)

Thus, cells can handle potentially toxic amounts of cysteine by a combination of thiol trapping, metabolic redistribution to non-reactive thiols and subsequent consumption for anabolism. (microbialcell.com)

use AMINO ACIDS, BRANCHED-CHAIN 1979, & KETO ACIDS & VALERATES 1973-1979 MH - 3-Hydroxyanthranilate 3,4-Dioxygenase UI - D050561 MN - D8.811.682.690.416.328 MS - An enzyme that catalyzes the conversion of 3-hydroxyanthranilate to 2-amino-3-carboxymuconate semialdehyde. (nih.gov)

This pathway visualises the conversion of methionine to cysteine, after which it is further metabolised in either an oxidative or non-oxidative manner. (wikipathways.org)

Persulfide Dioxygenase From Acidithiobacillus caldus: Variable Roles of Cysteine Residues and Hydrogen Bond Networks of the Active Site. (tu-darmstadt.de)

Pour chaque prélèvement, ces étapes ont été réalisées en parallèle avec des milieux supplémentés en hypotaurine (bras « H+ ») ou sans hypotaurine (bras « H- »). Après décongélation, nous avons mesuré la mobilité totale et progressive, la vitalité, l'intégrité de l'acrosome, des marqueurs de la voie de signalisation de la capacitation et la qualité nucléaire. (biomedcentral.com)
la première à démontrer les effets bénéfiques de la supplémentation en hypotaurine dans les milieux de préparation et de congélation sur la capacité de fécondation des spermatozoïdes humains et leur qualité nucléaire. (biomedcentral.com)

High cysteine levels inhibit ubiquitinylation, which lowers the rate of proteasomal degradation. (wikipedia.org)
In mammals and many other organisms, excess cysteine is believed to be primarily eliminated by the cysteine dioxygenase dependent oxidative degradation of cysteine, followed by the removal of the oxidative products. (microbialcell.com)

Hydroquinone increases 5-hydroxymethylcytosine formation through ten eleven translocation 1 (TET1) 5-methylcytosine dioxygenase. (nih.gov)
Ten-eleven translocation methylcytosine dioxygenase 1 (TET1), a CpG island binding protein, can repress gene expression by occupying hypomethylated CpG-rich promoters, and therefore SNCA could be a target for TET1. (einj.org)
Ten-eleven translocation methylcytosine dioxygenase 1 (TET1) acts as a repressor for α-synuclein gene ( SNCA ). (einj.org)

In this study the expression and purification of recombinant human indoleamine 2,3-dioxygenase (rhIDO) in E. coli(pQE-9-IDO, pREP4) was investigated in order to obtain high quality enzyme in high yields. (edu.au)
Expression and purification of indoleamine 2,3-dioxygenase -- 3. (edu.au)
Characterisation of indoleamine 2,3-dioxygenase -- 4. (edu.au)
Re-incorporation of heme into indoleamine 2,3-dioxygenase -- 5. (edu.au)
Mutagenesis of indoleamine 2,3-dioxygenase -- 6. (edu.au)

Organoseleno compounds have been observed, including selenomethionine, and possibly the newly-discovered S-(methylseleno)-cysteine molecule. (umass.edu)

Title: Placental and fetal cysteine dioxygenase gene expression in mouse gestation. (genscript.com)

Cysteine Dioxygenase Type 1 Inhibits Osteogenesis by Regulating Wnt Signaling in Primary Mouse Bone Marrow Stromal Cells. (genscript.com)
Title: Cysteine dioxygenase type 1 promotes adipogenesis via interaction with peroxisome proliferator-activated receptor gamma. (genscript.com)

Using targeted metabolite analysis, we observe that cysteine is initially rapidly interconverted to non-reactive cystine in vivo . (microbialcell.com)

protein_coding" "Cz03g38230.t1","No alias","Chromochloris zofingiensis","Glyoxalase/fosfomycin resistance/dioxygenase domain [Interproscan]. (ntu.edu.sg)

Critical regulator of cellular cysteine concentrations. (nih.gov)
Furthermore, high cysteine concentrations could possibly be neurotoxic and cytotoxic in rats [20,21] and high plasma cysteine concentrations in human beings had been connected with pre-eclampsia, early delivery, low delivery pounds and cardiovascular illnesses [22C24]. (health-ground.com)

These lines of proof suggest important jobs for CSE as the regulator of cysteine homoeostasis as well as the glutathioneCtaurine rheostat. (health-ground.com)

The contribution of individual cysteines towards the overall catalytic properties and stability of the rhIDO was evaluated through mutagenesis studies. (edu.au)
This study confirms that the Cys to Ala site-directed mutagenesis contributes to the changes in the kinetics of the mutated rhIDO, and that the cysteine moieties of rhIDO are involved in the normal catalytic function of the enzyme. (edu.au)

Crosslinking increases efficiency of CDO ten-fold and is regulated by levels of cysteine, an unusual example of protein cofactor formation mediated by substrate (feedforward activation). (wikipedia.org)

Homogentisate 1,2-Dioxygenase" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (umassmed.edu)

protein_coding" "AAC74417","ogt","Escherichia coli","O-6-alkylguanine-DNA:cysteine-protein methyltransferase [Ensembl]. (ntu.edu.sg)

Overall, the reaction involves addition of O2 to cysteine, which occurs spontaneously without enzyme catalysis. (wikipedia.org)
Cysteine (Cys) supply is directly related to the rate of GSH synthesis and its concentration represents the rate-limiting step for the first reaction (i.e., the formation of γ-glutamylcysteine). (medscape.com)

2006. Structure and mechanism of mouse cysteine dioxygenase. . (cornell.edu)

3. Cysteine Dioxygenase 1 Mediates Erastin-Induced Ferroptosis in Human gastric cancer Cells. (nih.gov)
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Cysteine And Glycine Rich Protein 1 (CSRP1) in Tissue homogenates and other biological fluids. (jemsec.com)

Description: A competitive ELISA for quantitative measurement of Rat Cysteine and glycine rich protein 1(CSRP1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. (jemsec.com)

In particular, CDO responds to changes in dietary cysteine availability and protein intake, maintaining decreased activity with low cysteine levels and increased activity at high levels to prevent cytotoxicity. (wikipedia.org)

This graph shows the total number of publications written about "Homogentisate 1,2-Dioxygenase" by people in this website by year, and whether "Homogentisate 1,2-Dioxygenase" was a major or minor topic of these publications. (umassmed.edu)

These observations support a role of cysteine in cancer progression, recurrence and chemoresistance. (biomedcentral.com)

Anatomy1

Chemicals and Drugs16

Sulfinic acid4

CDO11

Oxidase1

Enzymes3

Pathways4

Oxidation2

Glutamate-cystei3

Intracellular6

Biosynthesis1

Metabolism2

1.13.11.201

Catabolism1

Sulfur4

Metabolites2

Taurine3

Thiol1

1973-19791

Methionine1

Residues1

Hypotaurine2

Degradation2

TET13

Indoleamine5

Molecule1

Gene1

Type2

Metabolite1

Fosfomycin1

Concentrations2

Regulator1

Catalytic2

Cofactor1

Descriptor1

Escherichia1

Reaction2

Mechanism1

Human2

Description1

High1

Major1

Role1