• A bacterial artificial chromosome (BAC) is a DNA construct, based on a functional fertility plasmid (or F-plasmid), used for transforming and cloning in bacteria, usually E. coli. (wikipedia.org)
  • The bacterial artificial chromosome's usual insert size is 150-350 kbp. (wikipedia.org)
  • The development and applications of the bacterial artificial chromosome cloning system" (PDF). (wikipedia.org)
  • The vectors of this series contain genes of the moderate bacteriophage P1, which ensure replication of the phage chromosome in infected bacterial cells. (genomequebecplatforms.com)
  • However, unlike the bacteriophage, which, during a latent (lysogenic) state, integrates its chromosome into the chromosome of the host bacteriophage, the P1 phage maintains the chromosome in the cytoplasm of bacterial cells in the form of a circular covalently closed molecule resembling a plasmid, the size of which is 100 kbp. (genomequebecplatforms.com)
  • A bacterial artificial chromosome (BAC) is a vector system based on the E. coli F-plasmid, cos sites of the lambda phage and loxP of the P1 phage, used for cloning long (150-350 kbp) DNA sequences. (genomequebecplatforms.com)
  • A similar vector system called PAC (p1-derived artificial chromosome) was made on the basis of a bacterial P1 plasmid from the DNA of the P1 phage. (genomequebecplatforms.com)
  • Artificial bacterial chromosomes are often used to sequence the genomes of organisms in various projects, such as the Human Genome project. (genomequebecplatforms.com)
  • Bacterial artificial chromosomes (BACs) and P1-based artificial chromosomes (PACs) have proved excellent tools for the human genome sequencing projects. (ox.ac.uk)
  • We generated a transgenic (Tg) mouse line expressing Cre recombinase under the control of the Gpr88 promoter within a bacterial artificial chromosome clone. (shengsci.com)
  • Cosmid End-sequence profiling Fosmid Human artificial chromosome Secondary chromosome Yeast artificial chromosome O'Connor M, Peifer M, Bender W (June 1989). (wikipedia.org)
  • To overcome the difficulties arising when using artificial yeast chromosomes, alternative vector systems have been designed, among which the most popular at present are systems based on artificial chromosomes of bacteria - BAC. (genomequebecplatforms.com)
  • Chromosomes, Artificial, Yeast" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (harvard.edu)
  • Chromosomes in which fragments of exogenous DNA ranging in length up to several hundred kilobase pairs have been cloned into yeast through ligation to vector sequences. (harvard.edu)
  • This graph shows the total number of publications written about "Chromosomes, Artificial, Yeast" by people in Harvard Catalyst Profiles by year, and whether "Chromosomes, Artificial, Yeast" was a major or minor topic of these publication. (harvard.edu)
  • Below are the most recent publications written about "Chromosomes, Artificial, Yeast" by people in Profiles. (harvard.edu)
  • Any DNA molecule that has the ability to replicate inside the host to which the desired gene is integrated for cloning- include plasmids, bacteriophages, cosmids, BAC, yeast vectors, shuttle vectors etc. (biologyexams4u.com)
  • YAC (Yeast Artificial Chromosome): A vector of hundreds to kilobases long used for cloning of DNA fragment. (biologyexams4u.com)
  • At the loxP site, plasmid DNA can be cleaved by the Cre protein of the P1 phage, at the cos site by the corresponding enzyme of the lambda phage. (genomequebecplatforms.com)
  • BACs can also be utilized to detect genes or large sequences of interest and then used to map them onto the human chromosome using BAC arrays. (wikipedia.org)
  • Bacteriophages (phages), natural viruses known for infecting and killing bacteria, are the most diverse and abundant organisms on Earth. (cip.com.cn)
  • A similar cloning vector called a PAC has also been produced from the DNA of P1 bacteriophage. (wikipedia.org)
  • First artificial cloning vector (1977) constructed by Boliver & Rodriguez from E .coli plasmid. (biologyexams4u.com)
  • A short piece of DNA from the organism under study is inserted into the chromosome and then amplified and sequenced. (genomequebecplatforms.com)
  • In this study, we isolated and characterized the first bacteriophage infecting Oceanospirillum , vB_OliS_GJ44. (biomedcentral.com)
  • Combined with the eight environmental viral contigs from metagenomics, this study helps fill the gap in our understanding of the isolation, genomic and evolutionary development of Oceanospirillum bacteriophages and provides new insights into the interactions between hosts and bacteriophages for these important marine hydrocarbon-degrading microbial populations. (biomedcentral.com)
  • These artificial chromosomes are used extensively in molecular biology for the construction of comprehensive genomic libraries of higher organisms. (harvard.edu)
  • The size of the replicon, which is able to provide replication of the P1 chromosome in the lysogenic state, is only 1.5 kb. (genomequebecplatforms.com)
  • However, some phages, such as phage IFNA7 P1 and phage cp32 Cetirizine supplier from phage N15, phage KO2, and phage PY54 are a group of closely related phages that exist as linear plasmid-like prophages, with covalently closed hairpin ends (telomeres) due to the activity of a phage-encoded protein, protelomerase (12, 24, 54). (thetechnoant.info)
  • AGP ('A Golden Path') file is used to describe the instructions for building a contig , scaffold , or chromosome sequence. (nih.gov)
  • Pueden llevar grandes cantidades (cerca de 100-300 kilobases) de otras secuencias para una variedad de propósitos en bioingeniería. (bvsalud.org)
  • Cosmid End-sequence profiling Fosmid Human artificial chromosome Secondary chromosome Yeast artificial chromosome O'Connor M, Peifer M, Bender W (June 1989). (wikipedia.org)
  • Cre can be expressed in Tetrahymena and localizes to the macronucleus where it induces precise recombination at two loxP sequences in direct orientation in the Tetrahymena macronuclear chromosome. (biomedcentral.com)
  • One of the variant forms of a gene at a particular locus on a chromosome . (nih.gov)
  • The development and applications of the bacterial artificial chromosome cloning system" (PDF). (wikipedia.org)
  • This study provides valuable research material for further development of Proteus mirabilis bacteriophage biologics as promising alternatives to antibiotics, particularly in light of the growing challenge of antibiotic resistance posed by this bacterium. (bvsalud.org)