Filter...

AnatomyOrganismsDiseasesChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesTechnology, Industry, AgricultureInformation ScienceHealth Care

Select a category...

Chromatin Assembly Factor-1ChromatinChromatin Assembly and DisassemblyHistonesNucleosomesChromosomal Proteins, Non-HistoneNucleosome Assembly Protein 1Histone ChaperonesApc5 Subunit, Anaphase-Promoting Complex-CyclosomeRetinoblastoma-Binding Protein 4DNA ReplicationAcetylationNucleoplasminsDNA-Binding ProteinsSaccharomyces cerevisiae ProteinsChromatin ImmunoprecipitationProtein BindingNuclear ProteinsSaccharomyces cerevisiaeCell NucleusMolecular ChaperonesCell Cycle ProteinsDNA, SuperhelicalMicrococcal NucleaseMolecular Sequence DataTranscription, GeneticHistone AcetyltransferasesHeLa CellsHeterochromatinDNAVirus AssemblyMutationCell ExtractsTranscription FactorsCentromereAmino Acid SequenceMitosisGene SilencingProtein Structure, TertiaryS PhaseCell LinePromoter Regions, GeneticDrosophilaRetinoblastoma-Binding Protein 7Base SequenceBinding SitesXenopusAdenosine TriphosphatasesDNA DamageModels, BiologicalLysineDrosophila ProteinsProliferating Cell Nuclear AntigenRepressor ProteinsGene Expression Regulation, FungalXenopus laevisAcetyltransferasesHistone DeacetylasesMicrotubulesDeoxyribonuclease IOocytesEpigenesis, GeneticChromosomesMacromolecular SubstancesModels, MolecularFungal ProteinsDNA RepairGene Expression RegulationCell CycleCell-Free SystemProtein SubunitsRecombinant ProteinsKineticsActinsPlasmidsChromosomes, Artificial, MammalianRecombinant Fusion ProteinsAdenosine TriphosphateTwo-Hybrid System TechniquesPhosphorylationYeastsDimerizationDrosophila melanogasterSequence Homology, Amino AcidCarrier ProteinsMicroscopy, ElectronModels, GeneticTemplates, GeneticChromosome SegregationSex ChromatinChickensEmbryo, NonmammalianHigh Mobility Group ProteinsTubulinProtein-Serine-Threonine KinasesAutoantigensTelomereMultiprotein ComplexesCytoskeletonPhenotype

NucleosomesProteinsInduced chromatin structureNucleosome assemblyHeterochromatinMethylationRegulatesGenomeConformationSequencingRemodelingEukaryoticStructureDevelopmentalNuclearDamageShownNucleosomesLocal chromatinTranscriptionalHistonesOrganizationGenesSpecific chromatinDynamicsRegulationHeterochromatinProteinRole of histoneSubunitDifferentiationEpigenetic controlMolecularHistone chaperonesComplexesEnhancerPathwaysIntrinsicVariantsDamageHIRADefectsVitroCohesinChromosomesMachineryMutationsFacilitatesMethylation

Nucleosomes5

• The switch/sucrose nonfermenting (SWI/SNF) complex, first purified from yeast, is an adenosine triphosphate (ATP)-dependent chromatin-remodeling complex that regulates DNA accessibility by mobilizing nucleosomes in an ATP-dependent manner [ 1 ]. (biomedcentral.com)
• In eukaryotic cells, chromatin remodeling factors regulate chromatin structure by altering the assembly, disassembly, and relocalization of nucleosomes, thereby improving the local accessibility of transcription-related factors in their chromatin DNA, which further initiates or suppresses transcription of related genes. (creativebiomart.net)
• Nucleosome assembly proteins (NAPs) are histone chaperones that are crucial for the shuttling and incorporation of histones into nucleosomes. (biomedcentral.com)
• NAPs participate in the assembly and disassembly of nucleosomes thus contributing to chromatin structure organization. (biomedcentral.com)
• Short DNA fragments containing single, uniquely positioned nucleosome cores have been extensively employed as simple model experimental systems for analysis of many intranuclear processes, including binding of proteins to nucleosomes, transcription, DNA repair and ATP-dependent chromatin remodeling. (johnshopkins.edu)

Proteins2

• All living organisms package their DNA with chromatin proteins. (biologists.com)
• The human malaria parasite Plasmodium falciparum contains two nucleosome assembly proteins termed PfNapL and PfNapS. (biomedcentral.com)

Induced chromatin structure1

• item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Direct evidence for pitavastatin induced chromatin structure change in the KLF4 gene in endothelial cells. (soken.ac.jp)

Nucleosome assembly3

• In addition to playing an important role in histone exchange during nuclear processes, histone chaperones function in nucleocytoplasmic shuttling of histones, in histone storage, in nucleosome assembly and they act as a link between chromatin remodeling factors and histones [ 2 , 3 ]. (biomedcentral.com)
• In the present study, the structural basis of nucleosome assembly activity in P. falciparum was addressed by determining and analysing the crystal structure of smaller NAP in the parasite called PfNapS. (biomedcentral.com)
• May modulate chromatin structure by regulation of nucleosome assembly/disassembly (By similarity). (gao-lab.org)

Heterochromatin1

• Long before the identification of its molecular nature, it was recognized that chromatin exists in two main states: the densely stained heterochromatin contrasting with euchromatin ( Heitz, 1928 ). (biologists.com)

Methylation2

• It is mainly manifested in the reduction of whole-genome DNA methylation level, hypermethylation of the promoter region, changes in specific histone modification, abnormal chromatin conformation, changes in non-coding RNA (ncRNA) molecules, etc . (creativebiomart.net)
• Overall, our results suggest that hPaf1/PD2 in association with MLL1 regulates methylation of H3K4 residues, as well as interacts and regulates nuclear shuttling of chromatin remodeling protein CHD1, facilitating its function in pancreatic cancer cells. (nebraska.edu)

Regulates2

• USP51 is recruited to chromatin after DNA damage and regulates the dynamic assembly/disassembly of TP53BP1 and BRCA1. (creativebiomart.net)
• SNF5 (INI1/SMARCB1/BAF47) is a tumor suppressor that regulates the epigenome as a core member of the SWI/SNF chromatin remodeling complex. (elsevierpure.com)

Genome1

• By applying whole genome and quantitative chromatin conformation analysis {chromatin interaction analysis with paired end tag sequencing (ChIA-PET), and real time chromosome conformation capture (3C) assay}, we observed that the MEF2C-bound enhancer and transcription start site (TSS) of KLF4 came into closer spatial proximity by pitavastatin treatment. (soken.ac.jp)

Conformation2

• Taken together, dynamic chromatin conformation change was shown to mediate pitavastatin-responsive gene induction in endothelial cells. (soken.ac.jp)
• In the study of Ras-induced tumor development, the importance of chromatin remodeling changes in cancer is also evident, with the observation that chromatin conformation is more open in early-developing tumors and more accessible in late-stage tumor development. (creativebiomart.net)

Sequencing1

• We focused on MEF2C among the MEF2 family members and identified a novel functional MEF2C binding site 148 kb upstream of the KLF4 gene by chromatin immunoprecipitation along with deep sequencing (ChIP-seq) followed by luciferase assay. (soken.ac.jp)

Remodeling6

• Chromatin Remodeling of Colorectal Cancer Liver Metastasis is Mediated by an HGF-PU.1-DPP4 Axis. (duke.edu)
• Here it is shown that metastatic cells undergo specific chromatin remodeling in the liver. (duke.edu)
• The switch/sucrose nonfermenting (SWI/SNF) complex is an adenosine triphosphate-dependent chromatin-remodeling complex associated with the regulation of DNA accessibility. (biomedcentral.com)
• Change in gene expression associated with pancreatic cancer could be attributed to the variation in histone posttranslational modifications leading to subsequent remodeling of the chromatin template during transcription. (nebraska.edu)
• Also, a reduction in hPaf1 level resulted in reduced MLL1 expression and a corresponding decrease in the level of CHD1 (Chromohelicase DNA-binding protein 1), an ATPase dependent chromatin remodeling enzyme that specifically binds to H3K4 di and trimethyl marks. (nebraska.edu)
• Collectively, our results provide novel insight into the mechanisms of oncogenesis by demonstrating that disruption of a chromatin remodeling complex can largely, if not completely, substitute for genomic instability in the genesis of aggressive cancer. (elsevierpure.com)

Eukaryotic1

• In eukaryotic cells, DNA is present in a highly compacted form called chromatin. (biomedcentral.com)

Structure1

• Micrococcal nuclease digestion showed an altered chromatin structure in hPaf1/PD2-KD cells. (nebraska.edu)

Developmental1

• During dynamic developmental events, such as sexual reproduction, we propose that dramatic and rapid reconfiguration of source-sink relationships modifies chromatin states. (biologists.com)

Nuclear1

• Chromatin is highly dynamic, a characteristic that is vital in regulating nuclear processes such as transcription and replication which require access to DNA. (biomedcentral.com)

Damage1

• The bromodomain protein Brd4 insulates chromatin from DNA damage signalling. (duke.edu)

Shown1

• Chromatin regulation, in particular ATP-dependent chromatin remodelers, have previously been shown to be important in the regulation of reward-related behaviors in animal models of mental illnesses. (fsu.edu)

Nucleosomes6

• However, eukaryotic genome is highly packed in the form of chromatin that is an array of nucleosomes, each of which consists of two histone H2A-H2B dimers and one histone H3-H4 tetramer forming an octamer wrapped by about 146 bp (base pair) DNA double helix. (siu.edu)
• The differentiation of mouse naïve CD4 T cells into type 2 helper (Th2) cells is accompanied by chromatin remodeling at the nucleosomes associated with the IL-4, IL-13 and IL-5 genes. (ncku.edu.tw)
• The adenosine 5'-triphosphate (ATP)-dependent chromatin remodeler SMARCAD1 acts on nucleosomes during DNA replication, repair, and transcription, but despite its implication in disease, information on its function and biochemical activities is scarce. (bvsalud.org)
• Chromatin remodelers use the energy of ATP hydrolysis to slide nucleosomes, evict histones, or exchange histone variants. (bvsalud.org)
• During DNA replication, nucleosomes are rapidly assembled on newly synthesized DNA to restore chromatin organization. (omicsdi.org)
• Together with global binding analysis obtained by foxd3 biotin-ChIP and single cell profiles of foxd3-expressing premigratory NC, our analysis shows that, during early steps of NC formation, foxd3 acts globally as a pioneer factor to prime the onset of genes regulating NC specification and migration by re-arranging the chromatin landscape, opening cis-regulatory elements and reshuffling nucleosomes. (ox.ac.uk)

Local chromatin3

• Finally, DDB2 accumulation at 8-oxoG induces local chromatin unfolding. (nih.gov)
• All DNA-templated events, including replication and gene transcription, occur in the context of the local chromatin environment. (duke.edu)
• These histone variants dramatically alter the structure and dynamics of the local chromatin fiber resulting in epigenetic control of functions ranging from promoter accessibility to centromere identity. (nih.gov)

Transcriptional4

• 1. Rpp29 regulates histone H3.3 chromatin assembly through transcriptional mechanisms. (nih.gov)
• The mechanisms effecting establishment, maintenance, and modification of that specific physical conformation of CHROMATIN determining the transcriptional accessibility or inaccessibility of the DNA . (nih.gov)
• This work describes an elegant mechanism by which developmental experience influences adult phenotypes by establishing and maintaining transcriptional changes via RNAi and chromatin remodeling pathways. (syr.edu)
• The Mi-2/NuRD (NUcleosome Remodeling and histone Deacetylase) chromatin remodeling complex is a large heterogeneous multiprotein complex associated with transcriptional repression. (prinsesmaximacentrum.nl)

Histones5

• This histone modification is required for programmed DNA elimination, and like Pdd1p, these modified histones colocalize with chromatin containing the DNA sequences destined for elimination. (ciliate.org)
• In Arnold Stein's laboratory at Purdue University, she used classical chromatin biochemistry tools to understand how DNA sequence motifs and linker histones can shape the chromatin structure in silico, in vitro, and in vivo. (nih.gov)
• Here, we show that SMARCAD1 transfers the entire histone octamer from one DNA segment to another in an ATP-dependent manner but is also capable of de novo nucleosome assembly from histone octamer because of its ability to simultaneously bind all histones. (bvsalud.org)
• We show that these mutant histones are inefficiently deposited into chromatin in vivo. (omicsdi.org)
• We thus propose that TLK signalling promotes histone supply in S phase by targeting histone-free Asf1 and stimulating its ability to shuttle histones to sites of chromatin assembly. (omicsdi.org)

Organization1

• Taken together, these results demonstrate that foxd3 acts bimodally in the neural crest as a switch from "permissive" to "repressive" nucleosome and chromatin organization to maintain multipotency and define cell fates. (ox.ac.uk)

Genes2

• We report here that our recently developed method, Hi-TrAC, which specializes in detecting chromatin loops among accessible genomic regions, can detect active sub-TADs with a median size of 100 kb, most of which harbor one or two cell specifically expressed genes and regulatory elements such as super-enhancers organized into nested interaction domains. (nih.gov)
• The α-globin enhancers now interact with the flanking chromatin, upregulating expression of genes within this extended sub-TAD. (ox.ac.uk)

Specific chromatin2

• Deletion of selected sub-TAD boundaries have different impacts, such as decreased chromatin interaction and gene expression within the sub-TADs or compromised insulation between the sub-TADs, depending on the specific chromatin environment. (nih.gov)
• Th2-cell specific chromatin remodeling was induced at most of the Th2 cytokine gene loci. (ncku.edu.tw)

Dynamics1

• We focus on centromeres to elucidate the relationship between histone variants, chromatin fiber structure, assembly/disassembly dynamics and biological function. (nih.gov)

Regulation2

• 10. Regulation of chromatin assembly/disassembly by Rtt109p, a histone H3 Lys56-specific acetyltransferase, in vivo. (nih.gov)
• Chromatin regulation, in particular ATP-dependent chromatin remodelers, have previously been shown to be important in the regulation of reward-related behaviors in animal models of mental illnesses. (fsu.edu)

Heterochromatin1

• Human HIRA, ASF1a, ASF1b and CAF-1 are evolutionally conserved histone chaperones that form multiple functionally distinct chromatin-assembly complexes, with roles linked to diverse nuclear process, such as DNA replication and formation of heterochromatin in senescent cells. (omicsdi.org)

Protein1

• Inner nuclear protein Matrin-3 coordinates cell differentiation by stabilizing chromatin architecture. (umassmed.edu)

Role of histone1

• We primarily focus on the role of histone chaperones that control the assembly and disassembly chromatin. (ncl.ac.uk)

Subunit1

• However, the consequences of dual ATPase subunit loss on mSWI/SNF complex subunit composition, chromatin targeting, DNA accessibility and gene expression remain unknown. (nih.gov)

Differentiation1

• Differential Contributions of mSWI/SNF Chromatin Remodeler Sub-Families to Myoblast Differentiation. (umassmed.edu)

Epigenetic control2

• Importantly, the modulation of chromatin structure is a key mechanism for regulating the function of the underlying DNA and as such, chromatin represents a fundamental layer of epigenetic control. (ncl.ac.uk)
• The centromeric chromatin fiber presents a near-perfect model for studying epigenetic control, because no single DNA sequence is deemed necessary for formation, specification, maintenance or function of the centromere. (nih.gov)

Molecular1

• Together, these studies begin to define the molecular determinants of assembly of functionally diverse macromolecular histone chaperone complexes. (omicsdi.org)

Histone chaperones3

• In vivo study of the nucleosome assembly functions of ASF1 histone chaperones in human cells. (omicsdi.org)
• Histone chaperones have been implicated in nucleosome assembly and disassembly as well as histone modification. (omicsdi.org)
• ASF1 is a highly conserved histone H3/H4 chaperone that synergizes in vitro with two other histone chaperones, chromatin assembly factor 1 (CAF-1) and histone repression A factor (HIRA), in DNA synthesis-coupled and DNA synthesis-independent nucleosome assembly. (omicsdi.org)

Complexes1

• Using SMARCA4/2 ATPase mutant variants, we define the catalytic activity-dependent and catalytic activity-independent contributions of the ATPase module to the targeting of BAF and PBAF complexes on chromatin genome-wide. (nih.gov)

Enhancer1

• Tissue-specific CTCF-cohesin-mediated chromatin architecture delimits enhancer interactions and function in vivo. (ox.ac.uk)

Pathways1

• 20. All roads lead to chromatin: multiple pathways for histone deposition. (nih.gov)

Intrinsic1

• Histone variants were the next logical step in teasing out how intrinsic variability in the chromatin fiber can encode a diversity of biological functions. (nih.gov)

Variants1

• 7. PTMs on H3 variants before chromatin assembly potentiate their final epigenetic state. (nih.gov)

Damage1

• Cohesin mutations alter DNA damage repair and chromatin structure and create therapeutic vulnerabilities in MDS/AML. (umassmed.edu)

HIRA1

• Structure of a human ASF1a-HIRA complex and insights into specificity of histone chaperone complex assembly. (omicsdi.org)

Defects1

• Indeed, defects in chromatin are associated with gene dysregulation, genome instability and a number of diseases states that include cancers and neurodegenerative disorders. (ncl.ac.uk)

Vitro1

• She also studied how linker histone H1 could influence nucleosome positioning and chromatin folding in vitro and in vivo. (nih.gov)

Cohesin1

• Rather than acting solely as a barrier to chromatin modification, CTCF-cohesin boundaries in this sub-TAD delimit the region of chromatin to which enhancers have access and within which they interact with receptive promoters. (ox.ac.uk)

Chromosomes1

• Our collaborative projects range from developing new atomic force microscopy applications to enable fluorescent single molecule recognition within native chromatin, analyzing the structure of human artificial chromosomes, to mapping chromatin domains at fragile regions in human chromosomes. (nih.gov)

Machinery1

• The mechanisms involved with making the DNA in CHROMATIN more or less accessible to transcription machinery. (nih.gov)

Mutations1

• Five mutations in the rod domain (L85R, N195K, E358K, M371K and R386K)affected the assembly of the lamina. (biologists.com)

Facilitates1

• Activator targets co-activator that facilitates the assembly of general transcription factors (GTFs) and RNA polymerase II to form the pre-initiation complex (PIC) at the promoter for transcription initiation. (siu.edu)

Methylation1

• 5. A nucleosome turnover map reveals that the stability of histone H4 Lys20 methylation depends on histone recycling in transcribed chromatin. (nih.gov)

Send