Cho cells. Medical search. Frequent questions

As a result, accumulation of improperly folded proteins is a particularly challenging bottleneck in cell line engineering. (aiche.org)
It is expected research in this area will lead to improved engineering strategies for developing cell lines for higher titers of desired recombinant proteins, including relevant therapeutic protein products. (aiche.org)
IRIS medium is dedicated to maximal production of recombinant proteins with CHO DG44 cells. (thomassci.com)
BIOGRO-CHO SFM Supplement contains proteins and other components that require storage at -20ºC. (bioind.com)
We are a global provider of human and animal biospecimens: including frozen & FFPE tissue, DNA, RNA, total proteins, blood products and primary cells. (amsbio.com)
Therapeutic proteins, including monoclonal antibodies, are typically manufactured using clonally-derived, stable host cell lines, since consistent and predictable cell culture performance is highly desirable. (authorea.com)
Chinese Hamster Ovary (CHO) cells are the leading mammalian cell platform for manufacturing therapeutic proteins due to their ability for producing human-like products. (manchester.ac.uk)
Stable cell lines expressing recombinant proteins are generated by experienced scientists working on protein engineering, expression, and purification, and cell line development services for decades. (welgen.com)
Stable cell lines developed with our proprietary protein expression system with multiple selectable markers is very productive in expressing target proteins. (welgen.com)
MegaCD® CHO Growth Medium is a chemically-defined, hydrolysate-free, serum-free and non-animal origin basal medium designed to increase process yields of antibodies and recombinant proteins in Chinese Hamster Ovary (CHO) cells. (creative-bioarray.com)
The researchers have successfully produced Alpha-1-antitrypsin in CHO cells-one of the most deployed cell lines for production of human therapeutic proteins. (gohealthygo.com)
Chinese hamster ovary (CHO) cells have been used in biomanufacturing for decades because of their robust capacity to express a range of proteins, such as therapeutic enzymes and monoclonal antibodies (MAbs) at titers measured in multiple grams per liter of culture. (bioprocessintl.com)
Chinese hamster ovary (CHO) cells are one of the most useful host cell lines for the production of biopharmaceutical proteins. (elsevierpure.com)
The absence of any large macromolecules allows for isolation and purification of secreted proteins from the cells. (sigmaaldrich.com)
Translation factors are essential for cells to synthesize proteins, and their aberrant expression may result in pathological conditions including cancer. (cdc.gov)
Chimeric antigen receptors (CAR) recognize specific proteins on the surface of tumor cells. (msdmanuals.com)
In contrast to TCR T cells, CAR T cells recognize only relatively large proteins on the surface of tumor cells. (msdmanuals.com)

Purification of recombinant human growth hormone (rhGH) from Chinese hamster ovary (CHO) cell culture supernatant by Gradiflow large-scale electrophoresis is described. (edu.au)
Enzyme replacement using IV doses of recombinant human α-gal produced in CHO cells or in human fibroblasts is currently being evaluated in clinical trials as a potential therapy for this disease. (elsevierpure.com)
PeproTech's CHO cell-derived Recombinant Human sCD8α is a monomeric glycoprotein of 161 amino acid residues, which corresponds to the extracellular domain of CD8α. (peprotech.com)
When Recombinant SARS-CoV-2 Spike RBD Fc Chimera (Catalog # 10499-CV ) is immobilized at 0.2 µg/mL (100 µL/well), Biotinylated Recombinant Human ACE-2 Fc Chimera Avi-tag (CHO Expressed) (Catalog # AVI10544) binds with an ED 50 of 0.6-4.8. (rndsystems.com)
Biotinylated Recombinant Human ACE‑2 Fc Chimera Avi-tag (CHO Expressed) (Catalog # AVI10544) is measured by its ability to cleave a fluorogenic peptide substrate, Mca-YVADAPK(Dnp)-OH ( ES007 ). (rndsystems.com)

Transfection into mammalian cells is followed by screening for cell lines permanently expressing specific genes through their incorporation into the cellular genome. (welgen.com)
Due to the poor annotation of lncRNAs in CHO, the identification of potential targets with a described biological role required the comparison of human and mouse literature and databases, followed by alignment against the Chinese hamster genome, leading to predicted lncRNAs transcripts and previously un-annotated genomic regions (Table 1). (lncrnablog.com)
By employing our mouse RECON to the CHO-K1 genome in a comparative genomic approach, we could reconstruct the protein secretory pathway of CHO cells counting 764 CHO components. (biomedcentral.com)
We previously developed an accumulative site-specific gene integration system (AGIS) using Cre recombinase and mutated loxP sites for transgene integration and amplification in the CHO cell genome. (elsevierpure.com)
Here, we attempted to enhance transgene expression in recombinant CHO cells generated by AGIS using a production enhancer DNA element (PE) derived from the CHO genome. (elsevierpure.com)

Current work is focused on measuring the UPR, product titer, and product quality in a CHO cell line engineered for high productivity of IgG. (aiche.org)
The GeneBLAzer™ ADRA1A-NFAT-bla CHO-K1 cells contain the human Adrenergic Alpha-1A Receptor (ADRA1A), (Accession # NM_000680) stably integrated into the CellSensor™ NFAT-bla CHO-K1 cell line. (thermofisher.com)
He explained how his team interrogated the differences of cell line phenotypes by exploring changes in the genomic and transcriptomic levels using various sequencing methods. (genedata.com)
This talk was given as part of our regular Open Forums focused on biosafety and cell line development and is based on this publication . (genedata.com)
Biopharma and biotech professionals looking to advance rational cell line development by embracing digitalization and innovative enterprise data analysis systems. (genedata.com)
The Starter Pack is a bundle of Assay Complete reagents needed for culturing the specified cell line. (discoverx.com)
Starter Packs are a convenient, pre-packaged collection of Assay Complete reagents, including the relevant cell culture kit, cell plating reagent, freezing reagent, thawing reagent, and cell detachment reagent for the culture of the 93-0255C2 cell line. (discoverx.com)
In both cell line families, increased methylation was observed upon amplification. (bournemouth.ac.uk)
In the other cell line family, lower methylation frequency at the NFκB consensus binding site was accompanied by more NFκB recruitment to the promoter region. (bournemouth.ac.uk)
Metabolic profiling of recombinant CHO cells to identify potential targets for cell line engineering. (manchester.ac.uk)
Metabolomics data obtained through gas chromatography mass spectrometry (GS-MS) of a tetracycline (tet) inducible CHO T-RExTM cell line capable of expressing 0.09 pg/ul/cell EPO, revealed that 0.1% v/v tet addition does not affect consumption of carbon sources such as glucose, and pyruvate, but concentration of waste products, such as glycerol, threitol, alanine and phenyllactic acid increases towards the end of each batch culture. (manchester.ac.uk)
Our faster and more predictive screening techniques may significantly increase speed of stable cell line development while improving quality and uniformity. (welgen.com)
Stable cell line development study report. (welgen.com)
Stable cell line expressing human KVLQT1/minK. (mayflowerbio.com)
Cell line-specific, CHO DNA calibrators are included in the kit. (cygnustechnologies.com)
Group: Serum/protein free medium cell line. (cellosaurus.org)
With a recombinant alternative, you can both formulate the product, concentrate the protein, purify it, and avoid dependency of blood donors-so we would be able to ensure security of supply," says Bjørn Voldborg, director of CHO cell line development at the Novo Nordisk Foundation Center for Biosustainability. (gohealthygo.com)
This effort resulted in a cell line capable of producing over 1.2 grams pr. (gohealthygo.com)
Hence, this Alpha1-antitrypsin producing cell line is still only producing around 10 % of this known maximum. (gohealthygo.com)
They report on those lncRNAs present in a model host CHO cell line under batch and fed-batch conditions on two different days and relate the expression of different lncRNAs to each other. (lncrnablog.com)
Growth and culture viability of a CHO-S cell line in Batch and Fed-batch cultures were measured for 10 days and samples for RNA extraction taken at Day 4 and at Day 7. (lncrnablog.com)
Nowadays, you need to target productivities above 3 grams per litre for the production of monoclonal antibodies and a cell line selection shorter than 6 months for your CHO platform to be competitive. (gtp-bioways.com)
Using classic transfection techniques followed by genetic selection for the generation of your recombinant cell line is a time-consuming process often leading to an unpredictable outcome as transgene integration is a rare, random event. (gtp-bioways.com)
Catalent's patents protect, amongst other things, the culture of host cells comprising a retroviral vector for the expression of a protein of interest and the purification of the protein from such a cell line. (gtp-bioways.com)
In a recent poster, " Development of DoE based fed-batch strategies for high-producing CHO cell cultures ", presented by GE Healthcare and the Vienna Institute of BioTechnology, the authors developed and tested the benefit of a dynamic feed strategy that was responsive to the nutrient needs of a mAb-producing recombinant CHO cell line. (cellculturedish.com)
This in vitro study demonstrated that an encapsulated α-gal-secreting cell line can be used to treat Fabry mice by transplantation in vivo. (elsevierpure.com)
Subsequently a stable CHO cell line expressing the receptor fused at its C-terminal part with the enhanced green fluorescent protein (EGFP) was established, allowing to verify its cell surface distribution and to determine the affinity of various apelin and angiotensin fragments on the cloned receptor. (nih.gov)
As shown for the human APJ receptor, the rat apelin receptor expressed in the cell line was negatively coupled to adenylate cyclase. (nih.gov)
This superior transfection performance allows you to reduce the reagent dose, thus helping lower toxicity risk when transfecting your cell line. (thermofisher.com)
We were very happy and surprised to see Lipofectamine® 3000 reagent provide a more than 10-fold difference in transfection efficiency in our difficult-to-transfect cell line. (thermofisher.com)
In this webinar, Dr. Jenna Capyk provides a comparison of limiting dilution cloning and semi-solid cloning with respect to their application to cell line development and the probability of monoclonality for the cultures that are isolated using these methods. (stemcell.com)
Growing orders are recommended due to difficulties that can be experienced during the initial start-up of this cell line. (sigmaaldrich.com)
CRISPR/Cas9 gene editing for the creation of an MGAT1-deficient CHO cell line to control HIV-1 vaccine glycosylation. (bvsalud.org)
Epithelial cell line originally derived from porcine kidneys. (bvsalud.org)
This process theoretically provides a line of T cells with greater tumor specificity than those obtained from the blood. (msdmanuals.com)

Researchers at the University of Kent have undertaken the first landscape analysis of the lncRNA transcriptome in CHO using a mouse based microarray that also provided for the surveillance of the coding transcriptome. (lncrnablog.com)
Vito D, Smales CM. (2018) The Long Non-Coding RNA Transcriptome Landscape in CHO Cells Under Batch and Fed-Batch Conditions . (lncrnablog.com)

In attempts to isolate new CHO glycosylation mutants, selection protocols using plant lectins that bind galactose residues of cell surface carbohydrates were applied to mutagenized CHO populations. (elsevierpure.com)
Five of the mutants had properties typical of new CHO glycosylation mutants. (elsevierpure.com)
Normally, the CHO glycosylation profile of Alpha-1-antitrypsin is quite different from the human counterpart. (gohealthygo.com)
Furthermore, they removed a number of the CHO cells natural glycosylation genes using CRISPR-Cas9. (gohealthygo.com)

We used CHO fibroblasts stably expressing the beta1 subunit of the Na(+),K(+)-ATPase (CHO beta1), and studied the effect of ouabain on cell adhesion. (umassmed.edu)

Within the available suite of CHO cell lines, the glutamine synthetase knockout (GS-KO) selection system provides industry-leading speed to the identification of high-producing clones for use in biomanufacturing. (bioprocessintl.com)

Evaluation of site-specific methylation of the CMV promoter and its role in CHO cell productivity of a recombinant monoclonal antibody. (bournemouth.ac.uk)
We previously demonstrated that increased monoclonal antibody productivity in dihydrofolate reductase (DHFR)-amplified CHO cells correlates with phosphorylated transcription factor-cytomegalovirus (CMV) promoter interactions. (bournemouth.ac.uk)

The medium is developed for maximal recombinant protein production in suspension cells. (thomassci.com)
CHO transfected suspension cells have been carried for more than 2 years in IRIS medium with no loss of viability. (thomassci.com)
CHO suspension clonal stable cell lines are generated by electroporation using proprietary mammalian expression vector system with multiple selectable markers to express single gene of interest. (welgen.com)
This formulation has been specifically developed to support for high-density growth of CHO-K1, CHO-DG44, CHO-S and CHOZN cells in suspension. (creative-bioarray.com)
Take a sample of the cell suspension, e.g. 100μl, to count cells. (sigmaaldrich.com)
Centrifuge the cell suspension at low speed i.e. 100 - 150 x g for a maximum of 5 minutes. (sigmaaldrich.com)
VM), showed that the recovery of motor functions induced implanted either (1) as a solid piece in the lateral ven- by the grafted fetal dopamine neurons was well cor- tricle6 or a cortical cavity8 adjacent to the denervated related with the extent of graft-derived reinnervation caudate-putamen, or (2) as a crude cell suspension of the host caudate-putamen. (lu.se)

These media are intended for the growth of CHO cells of various kinds: CHO-KI, and transfected cells containing recombinant DNA related to the DHFR gene. (bioind.com)
In this article, we extend the characterization to include CMV promoter methylation and its influence on NFκB and CREB1 transcription factor binding to the CMV promoter in two families of DHFR-amplified CHO cell lines. (bournemouth.ac.uk)
Additionally, two DG44 cell lines containing inducible (pcDNA4/TO) or constitutive (pCDNA3.1) vectors (DG44-I and DG44-C cells respectively) with EPO and DHFR genes were generated. (manchester.ac.uk)

Chinese Hamster Ovary (CHO) cell lines are preferred host cells for therapeutic protein production. (nih.gov)
We applied this methodology to a dynamic model of Chinese hamster ovary (CHO) cell metabolism (Nolan, 2011). (tufts.edu)
The role of non-coding RNAs in determining growth, productivity, and recombinant product quality attributes in Chinese hamster ovary (CHO) cells has received much attention in recent years, exemplified by studies into microRNAs in particular. (lncrnablog.com)
Successful optimization of Chinese hamster ovary (CHO) cell culture for biomanufacturing has a significant impact on productivity, protein quality, manufacturing efficiency and economic feasibility. (cellculturedish.com)
To this end, ASIC1a current amplitudes and charge transport in transfected Chinese hamster ovary cells, and ASIC-mediated action potential signaling in cultured cortical neurons were measured in response to defined pH ramps of 1-40 s duration from pH 7.4 to pH 6.6 or 6.0. (frontiersin.org)
As a novel approach for treatment of Fabry disease we used polymer encapsulated Chinese hamster ovary (CHO) cells genetically modified to express α-gal. (elsevierpure.com)
Our group has shown that non-adherent chinese hamster ovary (CHO) cells transfected with the canine beta1 subunit become adhesive, and those homotypic interactions amongst beta1 subunits of the Na(+),K(+)-ATPase occur between neighboring epithelial cells. (umassmed.edu)
The RECON of the secretory pathway represents a strong tool for interpretation of data related to protein secretion as illustrated with transcriptomic data of Chinese Hamster Ovary (CHO) cells, the main platform for mammalian protein production. (biomedcentral.com)
EX-CELL ® CD CHO FUSION is a chemically defined, animal-component free medium developed for the long-term growth of Chinese Hamster Ovary (CHO) cells. (sigmaaldrich.com)
Chinese hamster ovary (CHO) is one of the most widely used non-human mammalian cell lines. (thermofisher.com)

Just weeks later, the firm saw regulatory success with Yescarta (axicabtagene ciloleucel), which became the second chimeric antigen receptor (CAR) T-cell therapy to be commercialized in the US. (bioprocessintl.com)
We report the use of multiplexed super-resolution imaging (Exchange-PAINT) followed by mean-shift clustering and random forest analysis to measure the precise distributions of five receptor tyrosine kinases (RTKs) from the ErbB, IGF-1R and Met families in breast cancer cells. (nature.com)
Here we exploit the multiplexing capabilities of Exchange-PAINT, a multiplexed variant of DNA-PAINT 14 , to image simultaneously five RTKs (EGFR, ErbB2, ErbB3, IGF-1R and Met) at endogenous levels of expression in BT20 cancer cells and to examine how receptor distribution changes following ligand stimulation. (nature.com)

These processes include transient transfection, and the creation of stable pools and clonal cell lines. (lonza.com)
Transient and stable pool processes are generally used for discovery through early-stage development to enable rapid production and screening of large numbers of protein candidates, while stable clonal cell lines are used for GMP clinical and commercial manufacturing. (lonza.com)
We performed the safety testing of our non-clonal cell bank, then used it to produce material at a 200L-scale for pre-clinical safety studies and formulation development work, and thereafter at 2000L scale for supply of material for a Phase 1 clinical trial. (authorea.com)
Distinct promoter methylation patterns arise upon clonal selection in different families of cell lines. (bournemouth.ac.uk)

liter of cultured CHO cells, thereby making it possible to produce Alpha-1-antitrypsin from CHO cells at a scale that can eliminate the need for human donors in the future. (gohealthygo.com)

2. Pilot small-scale transient transfection into HEK293 cells (Tuna293™ Process) or CHO cells (TunaCHO™ process) is performed, followed by the listed purification method to assess expression levels and protein yield. (curiaglobal.com)
Lipofectamine 3000 Transfection Reagent delivers a high efficiency for hard-to-transfect cell lines. (thermofisher.com)
It delivers exceptional transfection efficiency with improved cell viability for the widest range of hard-to-transfect and common cell types including HEK 293, HeLa, LNCaP, HepG2, and A549 cell lines (Figure 1) . (thermofisher.com)
Each reagent was used to transfect HEK 293, HeLa, LNCaP, HepG2, and A549 cell lines in a 96-well format, and GFP expression was analyzed 48 hours post-transfection. (thermofisher.com)
Lipofectamine 3000 reagent provided higher GFP transfection efficiency than Lipofectamine 2000 and FuGENE HD reagents for all five cell lines. (thermofisher.com)
The Lipofectamine 3000 transfection protocol was developed to be easy to use, while still ensuring optimum performance and reliability in a wide panel of cell lines. (thermofisher.com)
Lipofectamine 3000 reagent is a specially formulated gentle transfection reagent that is optimized for plasmid delivery into CHO cells with high efficiency and low cell toxicity. (thermofisher.com)
The easy protocol, exceptional plasmid DNA delivery, and the ability to effectively generate stable cell lines makes Lipofectamine 3000 reagent, the perfect reagent for CHO cell transfection experiments. (thermofisher.com)
Spectacular increase in transfection efficiency and dramatic decrease in cell toxicity. (thermofisher.com)

This thesis focused on characterizing the central carbon metabolism of different recombinant CHO cells to identify novel molecular mechanisms by increasing the metabolic load overexpressing human erythropoietin (EPO). (manchester.ac.uk)

ALiCE® is a radically different approach to cell-free expression and a kit unlike any others. (amsbio.com)
Glycerol, citrate and isotritate significantly increased in all the cell lines assessed upon EPO expression. (manchester.ac.uk)
The 3 shRNA plasmids will then be co-transfected into 293 cells with an cDNA expression plasmid. (welgen.com)
They then further analyzed the data to identify those lncRNAs whose expression changed the most between growth and stationary phases of culture or between batch and fed-batch culture to identify potential lncRNA targets for further functional studies with regard to their role in controlling growth of CHO cells. (lncrnablog.com)
At nanomolar non-inhibiting concentrations, ouabain affects the adhesive properties of epithelial cells by inducing the expression of cell adhesion molecules through the activation of signaling pathways associated with the alpha subunit. (umassmed.edu)
Immunofluorescence and biotinylation assays showed that ouabain (50 nM) increases the expression of the beta1 subunit of the Na(+),K(+)-ATPase at the cell membrane. (umassmed.edu)
The elucidation of the cell biology of the secretory machinery is of great importance, as it drives protein expression for biopharmaceutical industry, a 140 billion USD global market. (biomedcentral.com)
Recombinase-mediated site-specific gene integration into a predetermined chromosomal locus may enable predictable protein expression, reducing the laborious process of cell screening. (elsevierpure.com)
These results indicate that AGIS using the PE-flanked expression unit is a promising approach for establishing producer cell lines for biopharmaceutical protein production. (elsevierpure.com)
Presently, we have investigated the expression profile of TEF1A2 using a panel of human cancer cell lines and tumor tissues. (cdc.gov)
Collectively, these results indicated that: 1) TEFA12 is significantly overexpressed in human tumor cell lines and tumor tissue samples, 2) the cellular expression level of TEF1A2 may be used as a tumor marker, and 3) the anti-apoptotic role of TEF1A2 may potentially be responsible for its oncogenic function. (cdc.gov)
Concomitant use of interferon enhances the expression of major histocompatibility complex (MHC) antigens and TAAs on tumor cells, thereby augmenting the killing of tumor cells by the infused effector cells. (msdmanuals.com)

In order to measure ER stress in cell lines engineered for high-levels of recombinant protein production, this research aims to demonstrate the presence of ER stress associated with immunoglobulin G (IgG) antibody production, elucidate the progression of UPR in response to highly producing recombinant protein, and determine the effect of UPR on product quality. (aiche.org)

In order to trigger gene amplification of both genes, DG44-I and DG44-C cells were incubated and sub cultured in the presence of 250 nM MTX for 35 days, leading to EPO titers of 0.123 and 0.15 pg/ul/cell respectively without affecting maximum viable cell density (VCD). (manchester.ac.uk)

however , secretion levels needed for industrial cell lines likely leads to an imbalance in ER homeostasis, resulting in increased cellular stress. (aiche.org)
Affordable & consistent controls from 50 tumor cell lines, ideal for IHC, in situ hybridization & NGS applications. (amsbio.com)
Given the attractiveness of these data and our close relationship with the company Vectalys who offers a lentiviral techno l ogy based on patents owned by Institut Pasteur, we wanted to evaluate the possibility of developing efficient CHO biomanufacturing cell lines based on this technology for our clients. (gtp-bioways.com)
When performing transfections in hard-to-transfect cell lines, Lipofectamine 3000 reagent can be an ideal choice. (thermofisher.com)
Up to a 2000-fold overexpression of TEF1A2 was detected in 90% (nine out of 10) of the cancer cell lines derived from various human tissues compared to their corresponding controls. (cdc.gov)
Exposure of CHO cells to 2.5 and 10 um cadmium chloride for 24-hours resulted in significant apoptosis in all cell lines at 24- and 48-hours following the exposure. (cdc.gov)

A broad spectrum of radiolabeled peptides with high affinity for receptors expressed on tumor cells is currently under preclinical and clinical investigation for scintigraphic imaging and radionuclide therapy. (nih.gov)
ErbB receptors comprise a family of four RTKs (EGFR/ErbB1, Her2/ErbB2, Her3/ErbB3 and Her4/ErbB4) with important roles in normal cell physiology and in cancer 1 . (nature.com)
THP-1 cells have Fc and C3b receptors and lack surface and cytoplasmic immunoglobulins. (sigmaaldrich.com)
Cluster of differentiation 8 (CD8), a type I transmembrane glycoprotein of the immunoglobulin family of receptors, plays an integral role in signal transduction, and T cell differentiation and activation. (peprotech.com)

Using coculture system with Fabry fibroblasts, the secreted enzyme was taken up in cells, resulting in reduced accumulation of CTH in Fabry fibroblasts. (elsevierpure.com)

We also examined the effect of ouabain on the activation of signaling pathways in CHO beta1 cells, and their subsequent effect on cell adhesion. (umassmed.edu)

In addition, by controlling the number of lentiviral particles accessing the cell, you can achieve multiple gene insertions without any of the traditional amplification steps. (gtp-bioways.com)

Each reagent was used to transfect HeLa cells in a 96-well format at the indicated doses with an emerald green fluorescent protein (GFP)-expressing vector. (thermofisher.com)

This study demonstrates the ability of Gradiflow preparative electrophoresis technology to purify rhGH from mammalian cell culture supernatant in a one-step process with high purity and yield. (edu.au)
To achieve the highest performing cell culture, a fed-batch culture strategy is frequently used. (cellculturedish.com)
In this evaluation authors only selected the Cell Boost supplements that were beneficial for the overall culture performance. (cellculturedish.com)
Cho Cell Petri Dish2 Clip Art - Cell Culture Petri Dish is one of the clipart about red blood cell clipart,animal cell clip art,jail cell clipart. (clipartmax.com)
You can download (600x280) Cho Cell Petri Dish2 Clip Art - Cell Culture Petri Dish png clip art for free. (clipartmax.com)
Add thawed cells to a conical based centrifuge tube, e.g. 15ml tube, slowly add 4 ml of culture medium to the tube. (sigmaaldrich.com)
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. (rndsystems.com)
Lymphokine-activated killer (LAK) cells are produced from the patient's T cells which are extracted from the tumor and grown in a cell culture system with the lymphokine interleukin-2 (IL-2). (msdmanuals.com)
These cells are grown in culture in a manner similar to LAK cells. (msdmanuals.com)

We cloned the Tribolium inotocin preprohormone and the inotocin GPCR and expressed the GPCR in CHO cells. (lu.se)

The ED(50) was determined by the dose-dependentproliferation of human umbilical vein endothelial cells (HUVEC) using a concentration range of.0-10.0 ng/ml. (reprokine.com)
The human version of Alpha-1-antitrypsin has a very specific sugar-structure, whereas if produced in CHO cells exhibits a variety of sugars, which renders the protein ineffective in the human body. (gohealthygo.com)
Transgenic CHO cells permanently overexpressing TEF1A2 were developed and used to investigate the role of TEF1A2 in apoptosis induced by cadmium - a well-known human chemical carcinogen. (cdc.gov)
CAR T cell immunotherapy for human cancer. (msdmanuals.com)

Somatic Cell and Molecular Genetics , 16 (3), 211-223. (elsevierpure.com)
At these sites, which are a compound of stromal cells, extracellular matrix and soluble factors, complex molecular interactions that maintain the essential properties of stem cells occur, such as self-renewal and differentiation into multiple lineages, according to the organism's needs. (bvsalud.org)

BIOCHO-1 SFM Base is the basic formulation for CHO cells. (bioind.com)

Tumor cells are often found distant from the imaged mass. (medscape.com)
Once activated, CTLs play a crucial role in the clearance of pathogens and tumor cells. (peprotech.com)
A number of immunologic interventions, both passive and active, can be directed against tumor cells. (msdmanuals.com)
Антигени пухлин Many tumor cells produce antigens, which may be released in the bloodstream or remain on the cell surface. (msdmanuals.com)
TAAs) with high specificity to tumor cells. (msdmanuals.com)

Production of rhGH in CHO cells is an alternative to production in Escherichia coli, with the advantage that rhGH is secreted into protein-free production media, facilitating a more simple purification and avoiding resolubilization of inclusion bodies and protein refolding. (edu.au)
CHO and E.coli cells). (wikipedia.org)

Serum-Free Cell Freezing Medium (Cat. (bioind.com)
This solution is used for the cryopreservation of cells, which are to be grown in serum-free growth medium. (bioind.com)
In most cases it is possible to seed CHO cells that have been removed from freezing medium directly in the serum-free medium, when the cell concentration is at least 5x10 5 cells per 25cm 2 . (bioind.com)
The cells should be seeded with serum-free medium containing 5% serum and the serum concentration is then gradually reduced with each passage. (bioind.com)
After successful adaptation, it is recommended to cryopreserve the cells in Serum-Free Freezing Medium, in order to avoid the necessity of any further adaptation in the future. (bioind.com)
Then centrifuge, suspend the cells in serum-free medium and transfer them in the desired concentration. (bioind.com)

Histologically, NSCLC is divided into adenocarcinoma, squamous cell carcinoma (SCC) (see the image below), and large cell carcinoma. (medscape.com)
A cavitating right lower lobe squamous cell carcinoma. (medscape.com)

Understanding how stem cells behave in the niche is extremely important in order to extract these cells from their natural habitat, expand them in vitro and transplant the stem cells back to the patient, to repair and/or regenerate tissues and organs, with no risks to the individual's integrity. (bvsalud.org)

Adhesion is a crucial characteristic of epithelial cells to form barriers to pathogens and toxic substances from the environment. (umassmed.edu)
Epithelial cells attach to each other using intercellular junctions on the lateral membrane, including tight and adherent junctions, as well as the Na(+),K(+)-ATPase. (umassmed.edu)
Ouabain, a cardiotonic steroid, binds to the alpha subunit of the Na(+),K(+)-ATPase, inhibits the pump activity and induces the detachment of epithelial cells when used at concentrations above 300 nM. (umassmed.edu)

In addition, ADRA1A-NFAT-bla CHO-K1 cells have been tested for assay performance under variable conditions. (thermofisher.com)
Additional fee will be charged for Western blot or cell-based assay of your interest. (welgen.com)

CellSensor™ NFAT-bla CHO-K1 cells (Cat # K1078) contain a beta-lactamase (bla) reporter gene under control of the NFAT response element. (thermofisher.com)

Both ADRA1A-NFAT-bla CHO-K1 cells are functionally validated for Z'-factor and EC50 concentrations of Phenylephrine. (thermofisher.com)
Daily feed additions to replenish consumed nutrients substantially improved mAb and peak cell concentrations as well as viable cumulative cell days (VCCD) compared with batch cultivation. (cellculturedish.com)

CHO-CCK1 cell-derived tumors present in one flank of athymic mice accumulated 2.0% of injected (99m)Tc-sCCK8 per gram tissue at 1 h postinjection. (nih.gov)
Accumulation of (99m)Tc-sCCK8 and -nsCCK8 by CHO-CCK2 cell-derived tumors (present in the other flank) amounted to 4.2% and 0.6%, respectively. (nih.gov)
Chronic exposure of rats resulted in increased thyroid follicular cell tumors from sustained perturbation of thyroid hormone homeostasis. (cdc.gov)
Increasingly, renal cell cancers are diagnosed at an earlier stage, and nephron-sparing surgery and thermal ablation are gaining acceptance as a treatment of choice for smaller tumors. (medscape.com)

Prospective evaluation of analgesic use and risk of renal cell cancer. (medscape.com)

Likewise, these cells give rise to progenitor cells committed to a particular cell lineage, and play a crucial role in tissue repair and homeostasis. (bvsalud.org)
However, the progenitor cells consist of T cells isolated from resected tumor tissue. (msdmanuals.com)

Developed to maximize recombinant protein production of CHO transfected cells. (thomassci.com)
Simon's team explored the various applications of NGS for understanding cell phenotypes and their impact on the production of biologics. (genedata.com)
We have demonstrated the use of these methods to identify potential new and known targets for engineering improved growth and IgG production, as well as the general observation that CHO cells seem to have less strict transcriptional regulation of protein secretion than healthy mouse cells. (biomedcentral.com)
Although a series of production processes have been refined to improve protein productivity and cost performance, establishing producer cells is still time-consuming and labor-intensive. (elsevierpure.com)
THP-1 cells can also restore the response of purified T lymphocytes to Concanavlin A, show increased CO 2 production on phagocytosis and can be differentiated into macrophage-like cells using for example DMSO. (sigmaaldrich.com)
Ligation of MHC-I/peptide complexes presented by antigen-presenting cells (APCs), triggers the recruitment of lymphocyte-specific protein tyrosine kinase (Lck), which leads to lymphokine production, motility and cytotoxic T lymphocyte (CTL) activation. (peprotech.com)

The researchers demonstrate that the mouse microarray is suitable for the detection and analysis of thousands of CHO lncRNAs and validated a number of these by qRT-PCR. (lncrnablog.com)

The idea to use transplants of dopa- ment of protocols that allow generation of fully functional mine-producing cells to substitute for the lost midbrain and safe midbrain dopamine neurons from stem cells. (lu.se)
Niches are special microenvironments in tissue where stem cells are located. (bvsalud.org)
Dental pulp stem cells have been isolated from deciduous and permanent teeth and have the potential to self-renew and differentiate. (bvsalud.org)
However, little is known about the exact anatomic location of these cells, and the relationship between stem cells and surrounding cells in dental pulp. (bvsalud.org)
Therapies based on the application of stem cells have great potential in the prevention and treatment of several diseases, such as cancer, diabetes, cardiovascular disease, spinal cord injuries, neurological diseases such as Parkinson's and Alzheimer's, and in the regeneration of various tissues and organs. (bvsalud.org)
that is, they have the ability to generate other stem cells and perpetuate themselves. (bvsalud.org)

Used to measure residual CHO host cell DNA, this kit uses a 2 ml microfuge tube format and includes our proprietary extraction procedure, which does not use iodine or glycogen. (cygnustechnologies.com)

Renal cell carcinoma (see the image below) is the most common type of kidney cancer in adults. (medscape.com)
Renal cell carcinoma (RCC) accounts for approximately 3% of adult malignancies and 90-95% of neoplasms arising from the kidney. (medscape.com)

One of the new phenotypes was dominant in somatic cell hybrids, and the others were recessive. (elsevierpure.com)

Anatomy4

Organisms2

Chemicals and Drugs14

Analytical, Diagnostic and Therapeutic Techniques and Equipment4

Phenomena and Processes13

Information Science3

Proteins17

Recombinant human5

Genome5

Line35

Transcriptome2

Glycosylation4

Stably1

Glutamine synthetase1

Monoclonal antibody2

Suspension7

DHFR3

Ovary10

Receptor3

Clonal4

Liter1

Transfection9

Metabolism1

Expression12

Antibody1

DG441

Lines6

Receptors4

Fibroblasts1

Pathways1

Amplification1

Reagent1

Culture9

GPCR1

Human4

Molecular2

Formulation1

Tumor cells5

Coli2

Serum-free6

Squamous cell carc2

Vitro1

Epithelial3

Assay2

Gene1

Concentrations2

Tumors4

Renal cell c1

Progenitor cells2

Production6

Suitable1

Stem Cells6

Host1

Kidney2

Phenotypes1