A species of gram-negative, aerobic bacteria that is a fast-growing and soybean-nodulating innoculant.
A genus of gram-negative, aerobic, nonsporeforming rods which usually contain granules of poly-beta-hydroxybutyrate. (From Bergey's Manual of Determinative Bacteriology, 9th ed)
A species of gram-negative, aerobic bacteria that causes formation of root nodules on some, but not all, types of sweet clover, MEDICAGO SATIVA, and fenugreek.
The relationship between two different species of organisms that are interdependent; each gains benefits from the other or a relationship between different species where both of the organisms in question benefit from the presence of the other.
An annual legume. The SEEDS of this plant are edible and used to produce a variety of SOY FOODS.
The large family of plants characterized by pods. Some are edible and some cause LATHYRISM or FAVISM and other forms of poisoning. Other species yield useful materials like gums from ACACIA and various LECTINS like PHYTOHEMAGGLUTININS from PHASEOLUS. Many of them harbor NITROGEN FIXATION bacteria on their roots. Many but not all species of "beans" belong to this family.
The formation of a nitrogen-fixing cell mass on PLANT ROOTS following symbiotic infection by nitrogen-fixing bacteria such as RHIZOBIUM or FRANKIA.
A plant species of the family FABACEAE.
The process in certain BACTERIA; FUNGI; and CYANOBACTERIA converting free atmospheric NITROGEN to biologically usable forms of nitrogen, such as AMMONIA; NITRATES; and amino compounds.
A genus of gram-negative, aerobic, rod-shaped bacteria that activate PLANT ROOT NODULATION in leguminous plants. Members of this genus are nitrogen-fixing and common soil inhabitants.
A plant species of the family FABACEAE widely cultivated for ANIMAL FEED.
Polysaccharides found in bacteria and in capsules thereof.
The functional hereditary units of BACTERIA.
Proteins found in any species of bacterium.
Any of the processes by which cytoplasmic or intercellular factors influence the differential control of gene action in bacteria.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
A family of gram-negative bacteria which are saprophytes, symbionts, or plant pathogens.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
A plant genus of the family FABACEAE. It is distinct from Sweet Clover (MELILOTUS), from Bush Clover (LESPEDEZA), and from Red Clover (TRIFOLIUM).
A plant species of the family FABACEAE used to study GENETICS because it is DIPLOID, self fertile, has a small genome, and short generation time.

NolR regulates diverse symbiotic signals of Sinorhizobium fredii HH103. (1/28)

We have investigated in Sinorhizobium fredii HH103-1 (=HH103 Str(r)) the influence of the nolR gene on the production of three different bacterial symbiotic signals: Nod factors, signal responsive (SR) proteins, and exopolysaccharide (EPS). The presence of multiple copies of nolR (in plasmid pMUS675) repressed the transcription of all the flavonoid-inducible genes analyzed: nodA, nodD1, nolO, nolX, noeL, rhcJ, hesB, and y4pF. Inactivation of nolR (mutant SVQ517) or its overexpression (presence of pMUS675) altered the amount of Nod factors detected. Mutant SVQ517 produced Nod factors carrying N-methyl residues at the nonreducing N-acetyl-glucosamine, which never have been detected in S. fredii HH103. Plasmid pMUS675 increased the amounts of EPS produced by HH103-1 and SVQ517. The flavonoid genistein repressed EPS production of HH103-1 and SVQ517 but the presence of pMUS675 reduced this repression. The presence of plasmid pMUS675 clearly decreased the secretion of SR proteins. Inactivation, or overexpression, of nolR decreased the capacity of HH103 to nodulate Glycine max. However, HH103-1 and SVQ517 carrying plasmid pMUS675 showed enhanced nodulation capacity with Vigna unguiculata. The nolR gene was positively identified in all S. fredii strains investigated, S. xinjiangense CCBAU110, and S. saheli USDA4102. Apparently, S. teranga USDA4101 does not contain this gene.  (+info)

NopB, a soybean cultivar-specificity protein from Sinorhizobium fredii USDA257, is a type III secreted protein. (2/28)

The type III secretion system (TTSS) of plant- and animal-pathogenic bacteria is involved in translocation of virulence factors into the host cell cytosol where they modulate cellular processes. Sinorhizobium fredii USDA257 is a gram-negative soil bacterium that forms nitrogen-fixing nodules on specific soybean cultivars (Glycine max (L.) Merr.). This microsymbiont is known to secrete at least five nodulation outer proteins (Nops) in response to flavonoid induction. Some of these Nops have been shown to be secreted by TTSS in this symbiotic bacterium. We have isolated and purified an 18-kDa extracellular protein from flavonoid-induced cultures of USDA257. The N-terminal amino acid sequence of this purified protein was identical to the published sequence of the soybean cultivar-specificity protein, NopB (formerly NoIB). Inactivation of rhcN, which encodes an ATPase, abolished secretion of NopB. Similarly, a nonpolar nopB deletion mutant was compromised in its ability to secrete several Nops. A construct containing the coding region of nopB under control of a T7 promoter was expressed successfully in Escherichia coli and, subsequently, the recombinant NopB was purified by nickel-affinity column chromatography. Polyclonal antibodies raised against purified recombinant NopB were used in Western blot analysis to demonstrate the association of NopB with pilus-like surface appendages. Deletion analysis indicated that the first 33 N-terminal residues of NopB were necessary and sufficient to mediate the secretion of a green fluorescent protein reporter. Introduction of plasmid-borne extra copies of nopB into USDA257 resulted in lower accumulation of native NopB. We also show that USDA257 and its nonpolar nopB deletion mutant exhibited discernible differences in their ability to nodulate legume hosts.  (+info)

Sinorhizobium fredii USDA257 releases a 22-kDa outer membrane protein (Omp22) to the extracellular milieu when grown in calcium-limiting conditions. (3/28)

Calcium, which regulates a wide variety of cellular functions, plays an important role in Rhizobium-legume interactions. We investigated the effect of calcium on surface appendages of Sinorhizobium fredii USDA257. Cold-field emission scanning electron microscopy observation of USDA257 grown in calcium-limiting conditions revealed cells with unusual shape and size. Transmission electron microscopy observation revealed intact flagella were present only when USDA257 cells were grown in calcium-sufficient conditions. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of flagellar preparations from USDA257 cells grown in calcium-limiting conditions showed the presence of a 22-kDa protein that was absent from cells grown in calcium-sufficient conditions. We have cloned and determined the nucleotide sequence of the gene encoding the 22-kDa protein. After successful expression in Escherichia coli, polyclonal antibodies were raised against the recombinant 22-kDa protein (Omp22). Subcellular fractionation analysis demonstrated that Omp22 was predominantly present in the extracellular fraction. Western blot analysis revealed the presence of immunologically related proteins from diverse rhizobia. Immunocytochemical localization of thin sections of USDA257 cells showed specific labeling of protein A-gold particles on protein inclusions found proximal to the cells. Accumulation of Omp22 was greatly reduced when USDA257 cells were grown in the presence of increasing calcium. Northern blot analysis indicated that calcium was the only divalent cation among those tested that down-regulated omp22 expression. An omp22 mutant was able to grow in calcium-limiting conditions at a rate similar to that of wild-type USDA257. Significantly more nodules were initiated by the omp22 mutant than by the wild-type on soybean cultivar Peking grown in calcium-limiting conditions.  (+info)

Sinorhizobium fredii HH103 mutants affected in capsular polysaccharide (KPS) are impaired for nodulation with soybean and Cajanus cajan. (4/28)

The Sinorhizobium fredii HH103 rkp-1 region, which is involved in capsular polysaccharides (KPS) production, was isolated and sequenced. The organization of the S. fredii genes identified, rkpUAGHIJ and kpsF3, was identical to that described for S. meliloti 1021 but different from that of S. meliloti AK631. The long rkpA gene (7.5 kb) of S. fredii HH103 and S. meliloti 1021 appears as a fusion of six clustered AK631 genes, rkpABCDEF. S. fredii HH103-Rif(r) mutants affected in rkpH or rkpG were constructed. An exoA mutant unable to produce exopolysaccharide (EPS) and a double mutant exoA rkpH also were obtained. Glycine max (soybean) and Cajanus cajan (pigeon pea) plants inoculated with the rkpH, rkpG, and rkpH exoA derivatives of S. fredii HH103 showed reduced nodulation and severe symptoms of nitrogen starvation. The symbiotic capacity of the exoA mutant was not significantly altered. All these results indicate that KPS, but not EPS, is of crucial importance for the symbiotic capacity of S. fredii HH103-Rif(r). S. meliloti strains that produce only EPS or KPS are still effective with alfalfa. In S. fredii HH103, however, EPS and KPS are not equivalent, because mutants in rkp genes are symbiotically impaired regardless of whether or not EPS is produced.  (+info)

Y4xP, an open reading frame located in a type III protein secretion system locus of Sinorhizobium fredii USDA257 and USDA191, encodes cysteine synthase. (5/28)

Sinorhizobium fredii USDA257, a soybean symbiont, exports several nodulation outer proteins (Nops) into the rhizosphere. These proteins, which are exported by a type III secretion system (TTSS), have a pivotal role in host-specific nodulation. The entire TTSS of S. fredii lies within a 31-kb region that includes conserved genes that code for secretion machinery proteins, Nops, and several open reading frames (ORF) of unknown function. Identifying the functions of these ORF is essential to understand fully the role of TTSS in nodulation. Here, we report the characterization of y4xP, an ORF of previously unknown function. Southern blot analysis revealed that USDA257 contains two copies of y4xP, while a sibling, USDA191, contains a single copy. The amino acid sequence of Y4XP is homologous to both eukaryotic and prokaryotic cysteine synthase, a key enzyme in sulfur assimilation. The coding region of USDA257 y4xP under control of T7 promoter was expressed in Escherichia coli, and the recombinant protein was purified by nickel-affinity chromatography. Antibodies generated against soybean cysteine synthase cross-reacted with the recombinant protein. A nonpolar mutant of y4xP of USDA191 showed a marked reduction in cysteine synthase activity. Enzyme activity was completely restored when the mutant was complemented with a plasmid containing the y4xP sequence. Cysteine synthase activity was confined to the cell cytosol. Extracellular protein fraction from genistein-induced USDA191 showed no cysteine synthase activity. This observation indicates that cysteine synthase, which is located in the TTSS locus, is not a type III secreted protein. A nonpolar cysteine synthase mutant was able to export all the Nops to the rhizosphere, albeit in reduced amounts compared with the wild-type USDA191. Interestingly, USDA191 cysteine synthase mutant was able to initiate nodules on 'McCall' soybean more efficiently than the wild-type. Our results demonstrate that y4xP encodes a cysteine synthase and inactivation of this gene enhances the ability of USDA191 to form nodules on 'McCall' soybean by regulating Nops production.  (+info)

Inactivation of the Sinorhizobium fredii HH103 rhcJ gene abolishes nodulation outer proteins (Nops) secretion and decreases the symbiotic capacity with soybean. (6/28)

It has been postulated that nodulation outer proteins (Nops) avoid effective nodulation of Sinorhizobium fredii USDA257 to nodulate with American soybeans. S. fredii HH103 naturally nodulates with both Asiatic (non-commercial) and American (commercial) soybeans. Inactivation of the S. fredii HH103 gene rhcJ, which belongs to the tts (type III secretion) cluster, abolished Nop secretion and decreased its symbiotic capacity with the two varieties of soybeans. S. fredii strains HH103 and USDA257, that only nodulates with Asian soybeans, showed different SDS-PAGE Nop profiles, indicating that these strains secrete different sets of Nops. In coinoculation experiments, the presence of strain USDA257 provoked a clear reduction of the nodulation ability of strain HH103 with the American soybean cultivar Williams. These results suggest that S. fredii Nops can act as either detrimental or beneficial symbiotic factors in a strain-cultivar-dependent manner. Differences in the flavonoid-mediated expression of rhcJ with respect to nodA were also detected. In addition, one of the Nops secreted by strain HH103 was identified as NopA.  (+info)

Identification of a functional 2-keto-myo-inositol dehydratase gene of Sinorhizobium fredii USDA191 required for myo-inositol utilization. (7/28)

Sinorhizobium fredii USDA191 is a Gram-negative bacterium capable of forming nitrogen-fixing nodules on soybean roots. The USDA191 idhA gene encoding myo-inositol dehydrogenase, an enzyme necessary for myo-inositol utilization, is known to be involved in competitive nodulation and nitrogen fixation. In Bacillus subtilis, myo-inositol dehydrogenase catalyzes the first step of the myo-inositol catabolic pathway. Recently iolE was identified as the gene encoding 2-keto-myo-inositol dehydratase, which catalyzes the second step in the pathway. Here we report the presence of 2-keto-myo-inositol dehydratase activity in free-living USDA191 cells cultured in a medium containing myo-inositol. An iolE ortholog was cloned from USDA191. USDA191 iolE was expressed in Escherichia coli as a His(6)-tag fusion and purified to exhibit 2-keto-myo-inositol dehydratase activity. Inactivation of USDA191 iolE led to defective myo-inositol utilization. USDA191 iolE partially complemented a B. subtilis iolE deficient mutant. These results suggest that S. fredii USDA191 utilizes a myo-inositol catabolic pathway, analogous to that of B. subtilis, involving at least idhA and iolE.  (+info)

Evidence of horizontal transfer of symbiotic genes from a Bradyrhizobium japonicum inoculant strain to indigenous diazotrophs Sinorhizobium (Ensifer) fredii and Bradyrhizobium elkanii in a Brazilian Savannah soil. (8/28)

The importance of horizontal gene transfer (HGT) in the evolution and speciation of bacteria has been emphasized; however, most studies have focused on genes clustered in pathogenesis and very few on symbiosis islands. Both soybean (Glycine max [L.] Merrill) and compatible Bradyrhizobium japonicum and Bradyrhizobium elkanii strains are exotic to Brazil and have been massively introduced in the country since the early 1960s, occupying today about 45% of the cropped land. For the past 10 years, our group has obtained several isolates showing high diversity in morphological, physiological, genetic, and symbiotic properties in relation to the putative parental inoculant strains. In this study, parental strains and putative natural variants isolated from field-grown soybean nodules were genetically characterized in relation to conserved genes (by repetitive extragenic palindromic PCR using REP and BOX A1R primers, PCR-restriction fragment length polymorphism, and sequencing of the 16SrRNA genes), nodulation, and N(2)-fixation genes (PCR-RFLP and sequencing of nodY-nodA, nodC, and nifH genes). Both genetic variability due to adaptation to the stressful environmental conditions of the Brazilian Cerrados and HGT events were confirmed. One strain (S 127) was identified as an indigenous B. elkanii strain that acquired a nodC gene from the inoculant B. japonicum. Another one (CPAC 402) was identified as an indigenous Sinorhizobium (Ensifer) fredii strain that received the whole symbiotic island from the B. japonicum inoculant strain and maintained an extra copy of the original nifH gene. The results highlight the strategies that bacteria may commonly use to obtain ecological advantages, such as the acquisition of genes to establish effective symbioses with an exotic host legume.  (+info)

"Sinorhizobium fredii" is a gram-negative, rod-shaped bacterium that belongs to the family Rhizobiaceae. It has the ability to fix atmospheric nitrogen in a symbiotic relationship with certain leguminous plants, particularly soybeans and other related species. This bacterium infects the roots of these plants and forms nodules where it converts nitrogen gas into ammonia, a form that can be used by the plant for growth.

"Sinorhizobium fredii" is widely distributed in soil and is known to have a broad host range, including many important agricultural crops. It has been studied extensively due to its potential use as a biofertilizer, which could help reduce the need for chemical nitrogen fertilizers and improve soil health.

It's worth noting that while "Sinorhizobium fredii" is an important bacterium in agriculture and environmental science, it can also be a pathogen of some plants under certain conditions. Therefore, understanding its biology and ecology is crucial for optimizing its benefits and minimizing any potential negative impacts.

"Sinorhizobium" is a genus of bacteria that can form nitrogen-fixing nodules on the roots of certain leguminous plants, such as beans and alfalfa. These bacteria are able to convert atmospheric nitrogen into ammonia, which the plant can then use for growth. This symbiotic relationship benefits both the plant and the bacteria - the plant receives a source of nitrogen, while the bacteria receive carbon and other nutrients from the plant.

The genus "Sinorhizobium" is part of the family Rhizobiaceae and includes several species that are important for agriculture and the global nitrogen cycle. Some examples of "Sinorhizobium" species include S. meliloti, which forms nodules on alfalfa and other Medicago species, and S. fredii, which forms nodules on soybeans and other Glycine species.

It's worth noting that the taxonomy of nitrogen-fixing bacteria has undergone significant revisions in recent years, and some "Sinorhizobium" species have been reclassified as members of other genera. However, the genus "Sinorhizobium" remains a valid and important group of nitrogen-fixing bacteria.

"Sinorhizobium meliloti" is a species of nitrogen-fixing bacteria that forms nodules on the roots of leguminous plants, such as alfalfa and clover. These bacteria have the ability to convert atmospheric nitrogen into ammonia, which can then be used by the plant for growth and development. This symbiotic relationship benefits both the bacterium and the plant, as the plant provides carbon sources to the bacterium, while the bacterium provides the plant with a source of nitrogen.

"Sinorhizobium meliloti" is gram-negative, motile, and rod-shaped, and it can be found in soil and root nodules of leguminous plants. It has a complex genome consisting of a circular chromosome and several plasmids, which carry genes involved in nitrogen fixation and other important functions. The bacteria are able to sense and respond to various environmental signals, allowing them to adapt to changing conditions and establish successful symbioses with their host plants.

In addition to its agricultural importance, "Sinorhizobium meliloti" is also a model organism for studying the molecular mechanisms of symbiotic nitrogen fixation and bacterial genetics.

In the context of medicine and biology, symbiosis is a type of close and long-term biological interaction between two different biological organisms. Generally, one organism, called the symbiont, lives inside or on another organism, called the host. This interaction can be mutually beneficial (mutualistic), harmful to the host organism (parasitic), or have no effect on either organism (commensal).

Examples of mutualistic symbiotic relationships in humans include the bacteria that live in our gut and help us digest food, as well as the algae that live inside corals and provide them with nutrients. Parasitic symbioses, on the other hand, involve organisms like viruses or parasitic worms that live inside a host and cause harm to it.

It's worth noting that while the term "symbiosis" is often used in popular culture to refer to any close relationship between two organisms, in scientific contexts it has a more specific meaning related to long-term biological interactions.

I'm sorry for any confusion, but "soybeans" are not a medical term. They are a type of legume that is commonly used in agriculture and food production. The medical community might discuss soybeans in the context of nutrition or allergies, but there isn't a formal medical definition for this term.

Here's some general information: Soybeans, scientifically known as Glycine max, are native to East Asia and are now grown worldwide. They are a significant source of plant-based protein and oil. Soybeans contain various nutrients, including essential amino acids, fiber, B vitamins, and minerals like calcium, iron, magnesium, and zinc. They are used in various food products such as tofu, soy milk, tempeh, and miso. Additionally, soybeans are also used in the production of industrial products, including biodiesel, plastics, and inks. Some people may have allergic reactions to soybeans or soy products.

Fabaceae is the scientific name for a family of flowering plants commonly known as the legume, pea, or bean family. This family includes a wide variety of plants that are important economically, agriculturally, and ecologically. Many members of Fabaceae have compound leaves and produce fruits that are legumes, which are long, thin pods that contain seeds. Some well-known examples of plants in this family include beans, peas, lentils, peanuts, clover, and alfalfa.

In addition to their importance as food crops, many Fabaceae species have the ability to fix nitrogen from the atmosphere into the soil through a symbiotic relationship with bacteria that live in nodules on their roots. This makes them valuable for improving soil fertility and is one reason why they are often used in crop rotation and as cover crops.

It's worth noting that Fabaceae is sometimes still referred to by its older scientific name, Leguminosae.

Plant root nodulation is a type of symbiotic relationship between certain plants (mostly legumes) and nitrogen-fixing bacteria, such as Rhizobia species. This process involves the formation of specialized structures called nodules on the roots of the host plant. The bacteria inhabit these nodules and convert atmospheric nitrogen into ammonia, a form of nitrogen that plants can use for growth. In return, the plant provides the bacteria with carbon sources and a protected environment for growth. This mutualistic relationship helps improve soil fertility and promotes sustainable agriculture.

'Glycyrrhiza uralensis', also known as Chinese licorice, is a plant species native to Asia. In a medical context, it often refers to the root of this plant, which contains various compounds with potential medicinal properties. It has been used in traditional medicine for centuries to treat various health conditions such as respiratory disorders, liver diseases, and skin inflammations.

The active component of Glycyrrhiza uralensis is glycyrrhizin, which has anti-inflammatory, antiviral, and expectorant properties. However, it should be noted that excessive consumption of glycyrrhizin can lead to serious side effects such as hypertension, hypokalemia, and edema. Therefore, it is important to use this herb under the guidance of a healthcare professional.

Nitrogen fixation is a process by which nitrogen gas (N2) in the air is converted into ammonia (NH3) or other chemically reactive forms, making it available to plants and other organisms for use as a nutrient. This process is essential for the nitrogen cycle and for the growth of many types of plants, as most plants cannot utilize nitrogen gas directly from the air.

In the medical field, nitrogen fixation is not a commonly used term. However, in the context of microbiology and infectious diseases, some bacteria are capable of fixing nitrogen and this ability can contribute to their pathogenicity. For example, certain species of bacteria that colonize the human body, such as those found in the gut or on the skin, may be able to fix nitrogen and use it for their own growth and survival. In some cases, these bacteria may also release fixed nitrogen into the environment, which can have implications for the ecology and health of the host and surrounding ecosystems.

Rhizobium is not a medical term, but rather a term used in microbiology and agriculture. It refers to a genus of gram-negative bacteria that can fix nitrogen from the atmosphere into ammonia, which can then be used by plants as a nutrient. These bacteria live in the root nodules of leguminous plants (such as beans, peas, and clover) and form a symbiotic relationship with them.

The host plant provides Rhizobium with carbon sources and a protected environment within the root nodule, while the bacteria provide the plant with fixed nitrogen. This mutualistic interaction plays a crucial role in maintaining soil fertility and promoting plant growth.

While Rhizobium itself is not directly related to human health or medicine, understanding its symbiotic relationship with plants can have implications for agricultural practices, sustainable farming, and global food security.

'Medicago sativa' is the scientific name for a plant species more commonly known as alfalfa. In a medical context, alfalfa is often considered a herbal supplement and its medicinal properties include being a source of vitamins, minerals, and antioxidants. It has been used in traditional medicine to treat a variety of conditions such as kidney problems, asthma, arthritis, and high cholesterol levels. However, it's important to note that the effectiveness of alfalfa for these uses is not conclusively established by scientific research and its use may have potential risks or interactions with certain medications. Always consult a healthcare provider before starting any new supplement regimen.

Bacterial polysaccharides are complex carbohydrates that consist of long chains of sugar molecules (monosaccharides) linked together by glycosidic bonds. They are produced and used by bacteria for various purposes such as:

1. Structural components: Bacterial polysaccharides, such as peptidoglycan and lipopolysaccharide (LPS), play a crucial role in maintaining the structural integrity of bacterial cells. Peptidoglycan is a major component of the bacterial cell wall, while LPS forms the outer layer of the outer membrane in gram-negative bacteria.
2. Nutrient storage: Some bacteria synthesize and store polysaccharides as an energy reserve, similar to how plants store starch. These polysaccharides can be broken down and utilized by the bacterium when needed.
3. Virulence factors: Bacterial polysaccharides can also function as virulence factors, contributing to the pathogenesis of bacterial infections. For example, certain bacteria produce capsular polysaccharides (CPS) that surround and protect the bacterial cells from host immune defenses, allowing them to evade phagocytosis and persist within the host.
4. Adhesins: Some polysaccharides act as adhesins, facilitating the attachment of bacteria to surfaces or host cells. This is important for biofilm formation, which helps bacteria resist environmental stresses and antibiotic treatments.
5. Antigenic properties: Bacterial polysaccharides can be highly antigenic, eliciting an immune response in the host. The antigenicity of these molecules can vary between different bacterial species or even strains within a species, making them useful as targets for vaccines and diagnostic tests.

In summary, bacterial polysaccharides are complex carbohydrates that serve various functions in bacteria, including structural support, nutrient storage, virulence factor production, adhesion, and antigenicity.

A bacterial gene is a segment of DNA (or RNA in some viruses) that contains the genetic information necessary for the synthesis of a functional bacterial protein or RNA molecule. These genes are responsible for encoding various characteristics and functions of bacteria such as metabolism, reproduction, and resistance to antibiotics. They can be transmitted between bacteria through horizontal gene transfer mechanisms like conjugation, transformation, and transduction. Bacterial genes are often organized into operons, which are clusters of genes that are transcribed together as a single mRNA molecule.

It's important to note that the term "bacterial gene" is used to describe genetic elements found in bacteria, but not all genetic elements in bacteria are considered genes. For example, some DNA sequences may not encode functional products and are therefore not considered genes. Additionally, some bacterial genes may be plasmid-borne or phage-borne, rather than being located on the bacterial chromosome.

Bacterial proteins are a type of protein that are produced by bacteria as part of their structural or functional components. These proteins can be involved in various cellular processes, such as metabolism, DNA replication, transcription, and translation. They can also play a role in bacterial pathogenesis, helping the bacteria to evade the host's immune system, acquire nutrients, and multiply within the host.

Bacterial proteins can be classified into different categories based on their function, such as:

1. Enzymes: Proteins that catalyze chemical reactions in the bacterial cell.
2. Structural proteins: Proteins that provide structural support and maintain the shape of the bacterial cell.
3. Signaling proteins: Proteins that help bacteria to communicate with each other and coordinate their behavior.
4. Transport proteins: Proteins that facilitate the movement of molecules across the bacterial cell membrane.
5. Toxins: Proteins that are produced by pathogenic bacteria to damage host cells and promote infection.
6. Surface proteins: Proteins that are located on the surface of the bacterial cell and interact with the environment or host cells.

Understanding the structure and function of bacterial proteins is important for developing new antibiotics, vaccines, and other therapeutic strategies to combat bacterial infections.

Gene expression regulation in bacteria refers to the complex cellular processes that control the production of proteins from specific genes. This regulation allows bacteria to adapt to changing environmental conditions and ensure the appropriate amount of protein is produced at the right time.

Bacteria have a variety of mechanisms for regulating gene expression, including:

1. Operon structure: Many bacterial genes are organized into operons, which are clusters of genes that are transcribed together as a single mRNA molecule. The expression of these genes can be coordinately regulated by controlling the transcription of the entire operon.
2. Promoter regulation: Transcription is initiated at promoter regions upstream of the gene or operon. Bacteria have regulatory proteins called sigma factors that bind to the promoter and recruit RNA polymerase, the enzyme responsible for transcribing DNA into RNA. The binding of sigma factors can be influenced by environmental signals, allowing for regulation of transcription.
3. Attenuation: Some operons have regulatory regions called attenuators that control transcription termination. These regions contain hairpin structures that can form in the mRNA and cause transcription to stop prematurely. The formation of these hairpins is influenced by the concentration of specific metabolites, allowing for regulation of gene expression based on the availability of those metabolites.
4. Riboswitches: Some bacterial mRNAs contain regulatory elements called riboswitches that bind small molecules directly. When a small molecule binds to the riboswitch, it changes conformation and affects transcription or translation of the associated gene.
5. CRISPR-Cas systems: Bacteria use CRISPR-Cas systems for adaptive immunity against viruses and plasmids. These systems incorporate short sequences from foreign DNA into their own genome, which can then be used to recognize and cleave similar sequences in invading genetic elements.

Overall, gene expression regulation in bacteria is a complex process that allows them to respond quickly and efficiently to changing environmental conditions. Understanding these regulatory mechanisms can provide insights into bacterial physiology and help inform strategies for controlling bacterial growth and behavior.

Molecular sequence data refers to the specific arrangement of molecules, most commonly nucleotides in DNA or RNA, or amino acids in proteins, that make up a biological macromolecule. This data is generated through laboratory techniques such as sequencing, and provides information about the exact order of the constituent molecules. This data is crucial in various fields of biology, including genetics, evolution, and molecular biology, allowing for comparisons between different organisms, identification of genetic variations, and studies of gene function and regulation.

Rhizobiaceae is a family of bacteria that have the ability to fix nitrogen. These bacteria are gram-negative, motile, and rod-shaped. They are commonly found in the root nodules of leguminous plants, where they form a symbiotic relationship with the plant. The bacteria provide the plant with fixed nitrogen, while the plant provides the bacteria with carbon and a protected environment.

The most well-known genus of Rhizobiaceae is Rhizobium, which includes several species that are important for agriculture because of their ability to fix nitrogen in the root nodules of legumes. Other genera in this family include Bradyrhizobium, Mesorhizobium, and Sinorhizobium.

It's worth noting that while Rhizobiaceae bacteria are generally beneficial, they can sometimes cause disease in plants under certain conditions. For example, some strains of Rhizobium can cause leaf spots on certain crops.

A mutation is a permanent change in the DNA sequence of an organism's genome. Mutations can occur spontaneously or be caused by environmental factors such as exposure to radiation, chemicals, or viruses. They may have various effects on the organism, ranging from benign to harmful, depending on where they occur and whether they alter the function of essential proteins. In some cases, mutations can increase an individual's susceptibility to certain diseases or disorders, while in others, they may confer a survival advantage. Mutations are the driving force behind evolution, as they introduce new genetic variability into populations, which can then be acted upon by natural selection.

"Medicago" is a genus of flowering plants in the family Fabaceae, also known as the legume or pea family. It includes several species that are important forage crops and green manure, such as Medicago sativa (alfalfa or lucerne) and Medicago lupulina (black medic). These plants have the ability to fix nitrogen from the atmosphere through their root nodules, which benefits the soil and other nearby plants. They are often used in rotational grazing systems and for erosion control.

'Medicago truncatula' is not a medical term, but a scientific name for a plant species. It is commonly known as barrel medic or yellow trefoil and is native to the Mediterranean region. It is a model organism in the field of plant genetics and molecular biology due to its small genome size and ease of transformation. While it does not have direct medical applications, studies on this plant can contribute to our understanding of fundamental biological processes and may have indirect implications for human health.

Sinorhizobium fredii NGR234). Find diseases associated with this biological target and compounds tested against it in bioassay ...
Sinorhizobium fredii exhibit a broad host-range and are able to nodulate both determinant hosts such as soy as well as ... Ensifer fredii is a nitrogen fixing bacteria of the genus Sinorhizobium. It is a fast-growing root nodule bacteria. ... "Genome Sequence of the Soybean Symbiont Sinorhizobium fredii HH103". Journal of Bacteriology. 194 (6): 1617-1618. doi:10.1128/ ... "Bacterial Molecular Signals in the Sinorhizobium fredii-Soybean Symbiosis". International Journal of Molecular Sciences. 17 (5 ...
The Sinorhizobium fredii HH103 Genome: A Comparative Analysis With S. fredii Strains Differing in Their Symbiotic Behavior With ... Sinorhizobium fredii HH103 nolR and nodD2 mutants gain capacity for infection thread invasion of Lotus japonicus Gifu and Lotus ... The Sinorhizobium fredii HH103 type III secretion system effector NopC blocks nodulation with Lotus japonicus Gifu. I Jim nez- ... Sinorhizobium fredii HH103 Invades Lotus burttii by Crack Entry in a Nod Factor-and Surface Polysaccharide-Dependent Manner. S ...
GunA de Sinorhizobium (Ensifer) fredii HH103 es una celulasa secretada a trav s del sistema de secreci n de tipo 3 que afecta ... Sinorhizobium ((Ensifer) Fredii y su Interacci n Simbi tica con las Leguminosas. Pag. 113-123. En: Fijaci n de Nitr geno: ... Regulaci n de la Secreci n de Nops a Trav s del Sistema de Secreci n de Tipo III de Sinorhizobium Fredii Hh103. Ponencia en ... Svq120: a Sinorhizobium Fredii Strain Hh103 Mutant in the Hrcq Gene Located in the Type III Secretion Protein Gene Region. ...
... and the high-affinity phosphate transporter PstSCAB is known to promote the efficiency of the Sinorhizobium fredii-soybean ... On the other hand, an IAA overproducing mutant of Sinorhizobium meliloti increased nodulation in Medicago sp. when compared to ... 2004) also found that an ACC deaminase-producing Sinorhizobium meliloti mutant elicited 40% more nodulation in Madicago sativa ... For example, mutants of Sinorhizobium meliloti containing additional copies of the ribBA genes were found to exhibit increased ...
Sinorhizobium B03.440.400.425.700.887.249 Sinorhizobium fredii B03.440.400.425.700.887.500 Sinorhizobium meliloti B03.440. ... Sinorhizobium B03.660.050.662.835.399 Sinorhizobium fredii B03.660.050.662.835.800 Sinorhizobium meliloti B03.660.050.750 ...
... such as A0A249PTX6 of Sinorhizobium fredii (Supplementary Fig. 3b). Although these sequences consist of regular repeats of the ...
Structural analysis of the capsular polysaccharide from Sinorhizobium fredii HWG35. MA Rodr guez-Carvajal, JA Rodrigues, ME ...
2011) The conjugative plasmid of a bean-nodulating Sinorhizobium fredii strain is assembled from sequences of two Rhizobium ... Deep sequencing of the cryptic plasmid mobilome in the symbiotic bacterium Sinorhizobium meliloti. ... plasmids and the chromosome of a Sinorhizobium strain. BMC Microbiol 11:149. Torres Tejerizo GA et al. (2011) First genomic ...
Sinorhizobium fredii - Preferred Concept UI. M0440647. Scope note. A species of gram-negative, aerobic bacteria that is a fast- ... Sinorhizobium fredii. Scope note:. Especie de bacterias gramnegativas aerobias, de rápido crecimiento y que es un inoculante de ... Sinorhizobium fredii Descriptor Spanish: Sinorhizobium fredii Spanish from Spain Descriptor. ... Sinorhizobium fredii Descriptor French: Sinorhizobium fredii Tree number(s):. B03.440.400.425.700.887.249. B03.660.050.662. ...
Sinorhizobium [B03.440.400.425.700.887] * Sinorhizobium fredii [B03.440.400.425.700.887.249] * Sinorhizobium meliloti [B03.440. ... Sinorhizobium [B03.660.050.662.835] * Sinorhizobium fredii [B03.660.050.662.835.399] * Sinorhizobium meliloti [B03.660.050.662. ... Sinorhizobium fredii Preferred Term Term UI T524159. Date10/24/2002. LexicalTag NON. ThesaurusID NLM (2004). ... Sinorhizobium fredii Preferred Concept UI. M0440647. Registry Number. txid380. Scope Note. A species of gram-negative, aerobic ...
Sinorhizobium [B03.440.400.425.700.887] * Sinorhizobium fredii [B03.440.400.425.700.887.249] * Sinorhizobium meliloti [B03.440. ... Sinorhizobium [B03.660.050.662.835] * Sinorhizobium fredii [B03.660.050.662.835.399] * Sinorhizobium meliloti [B03.660.050.662. ... Sinorhizobium fredii Preferred Term Term UI T524159. Date10/24/2002. LexicalTag NON. ThesaurusID NLM (2004). ... Sinorhizobium fredii Preferred Concept UI. M0440647. Registry Number. txid380. Scope Note. A species of gram-negative, aerobic ...
1 Bacteria Sinorhizobium fredii HH103 CCE98600.1 ncbi GH51_1 Bacteria Sinorhizobium fredii NXT3 AUX75831.1 ncbi GH51_1 Bacteria ... Sinorhizobium fredii USDA 257 AFL49813.1 ncbi GH51_1 Bacteria Sinorhizobium garamanticum LMG 24692 WEX89815.1 ncbi GH51_1 ... CCBAU 05631 ASY55884.1 ncbi GH51_1 Bacteria Sinorhizobium sp. M103 WEJ13157.1 ncbi GH51_1 Bacteria Sinorhizobium terangae ... R1AF57 ASN50729.1 ncbi GH51_1 Bacteria Sinorhizobium alkalisoli YIC4027 QFI65705.1 ncbi GH51_1 Bacteria Sinorhizobium ...
Sinorhizobium fredii NGR234. NGR_c18610 (GI 227822406). Response regulator, FrzZ family. 2 Response_reg. Genomic Context. ... Sinorhizobium fredii HH103. SFHH103_01786 (GI 378826376). Response regulator, FrzZ family. 2 Response_reg. Genomic Context. ... Sinorhizobium fredii USDA 257. USDA257_c42720 (GI 398354104). Response regulator, FrzZ family. 2 Response_reg. Genomic Context ... Sinorhizobium meliloti Rm41. BN406_01729 (GI 407720938). Response regulator, FrzZ family. 2 Response_reg. Genomic Context. two ...
gi,255767010,ref,NC_000914.2,:c533172-532723 Sinorhizobium fredii NGR234 plasmid pNGR234a, complete sequence. ... gi,16519688,ref,NP_443808.1, conjugal transfer protein TrbE [Sinorhizobium fredii NGR234]. ...
Lotus , Sinorhizobium fredii , Sinorhizobium , Proteínas de Bactérias/genética , Nodulação , Sinorhizobium fredii/genética , ... Nitrito Redutases/genética , Fixação de Nitrogênio/genética , Sinorhizobium fredii/genética , Sinorhizobium fredii/metabolismo ... Sinorhizobium fredii/genética , Sinorhizobium fredii/metabolismo , Soja/microbiologia , Sistemas de Secreção Tipo III/genética ... Sinorhizobium fredii/genética , Sinorhizobium fredii/imunologia , Espectrometria de Massas por Ionização e Dessorção a Laser ...
Sinorhizobium fredii CCBAU 83666 chromosome, complete genome.. 3986633. DNA. Sinorhizobium fredii CCBAU 83666. ... Sinorhizobium sp. CCBAU 05631 chromosome, complete genome.. 4018433. DNA. Sinorhizobium sp. CCBAU 05631. ...
Sinorhizobium fredii USDA 205. Type Strain. ATCC® 35423™. June 1, 2021. 6.8 Mb. Download. ... Sinorhizobium meliloti. Type Strain. ATCC® 9930™. October 28, 2020. 7.4 Mb. Download. ...
Host Lineage: Sinorhizobium fredii; Sinorhizobium; Rhizobiaceae; Rhizobiales; Proteobacteria; Bacteria. General Information: ... Query: NC_018000:2054098:2072228 Sinorhizobium fredii USDA 257 chromosome, complete genome. Start: 2072228, End: 2073184, ...
Sinorhizobium fredii HH103, complete genome. site-specific recombinase. 2e-11. 70.5. NC_007493:1672163:1704748. 1704748. ...
Sinorhizobium fredii NGR234 and Sinorhizobium fredii NGR234 (pPAT) were allowed to grow in YEM medium for 70 hours with proper ... Sinorhizobium fredii NGR234 (pPAT) and Sinorhizobium fredii NGR234 cells were grown in YEM broth with the appropriate ... Sinorhizobium fredii NGR234 (pPAT) showed less fluoroscence compared to Sinorhizobium fredii NGR234. For further confirmation ... Equal number of Sinorhizobium fredii NGR234 and Sinorhizobium fredii NGR234 (pPAT) cells were taken and stained which showed ...
Sinorhizobium fredii strain SSR1 16S ribosomal RNA gene, partial sequence. 2012 Enhanced yield and growth of pigeon pea by ... Sinorhizobium abri gene for 16S rRNA, partial sequence, strain: HA-1. 1998 Sinorhizobium indiaense sp. nov. and Sinorhizobium ... Isolation of Sinorhizobium meliloti RMP66 from Mucuna pruriens L. 29 JN546145 Prabha,C. and Maheshwari, D.K. Sinorhizobium ... Sinorhizobium fredii strain KCC5 16S ribosomal RNA gene, partial sequence. 2009 Genetic diversity of rhizobia from Cajanus ...
Sinorhizobium fredii. Sinorhizobium fredii. Sinorhizobium fredii. Sphaerotilus. Sphaerotilus. Sphaerotilus. Sphingobacterium. ...
مسیر حس‌گر حد نصاب در باکتری Sinorhizobium meliloti حداقل از سه ژن sinI ، sinR و expR تشکیل یافته است و در کنترل فعالیت‌های ... Regulation and symbiotic significance of nodulation outer proteins secretion in Sinorhizobium fredii HH103. Microbiology 2008; ... QS system of Sinorhizobium meliloti composed of at least three genes of sinI (autoinducer synthase), sinR and expR (autoinducer ... مسیر حس‌گر حد نصاب در باکتری Sinorhizobium meliloti حداقل از سه ژن sinI ، sinR و expR تشکیل یافته است و در کنترل فعالیت‌های ...
Sinorhizobium fredii and Sinorhizobium meliloti produce structurally conserved lipopolysaccharides and strain-specific K ... The Sinorhizobium fredii HH103 lipopolysaccharide is not only relevant at early soybean nodulation stages but also for ... Sinorhizobium meliloti acpXL mutant lacks the C28 hydroxylated fatty acid moiety of lipid A and does not express a slow ... The Sinorhizobium meliloti essential porin RopA1 is a target for numerous bacteriophages.. Crook MB, Draper AL, Guillory RJ, ...
SYMBIOTIC EFFECTIVENESS OF BRADYRHIZOBIUM JAPONICUM USDA 110 AND SINORHIZOBIUM FREDII USDA 191 ON TWO DIFFERENT SOYBEAN ...
Sinorhizobium) fredii NGR234 , Microbial Ecology, Microbial Ecology,,,. ... fredii NGR234. Microbial Ecology. 83(4): 1008-1025.. 6. Bellamkonda R, Sarma PVSRN, Ankati S, Bhuvanachandra B, Podile AR (2021 ...
Sinorhizobium) fredii NGR234 [Mass Spectrometry - Proteomics Facility]. Microbial Ecology. :1-18.*PubMed ...

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