A species of gram-negative, aerobic bacteria that causes formation of root nodules on some, but not all, types of sweet clover, MEDICAGO SATIVA, and fenugreek.
A genus of gram-negative, aerobic, nonsporeforming rods which usually contain granules of poly-beta-hydroxybutyrate. (From Bergey's Manual of Determinative Bacteriology, 9th ed)
A species of gram-negative, aerobic bacteria that is a fast-growing and soybean-nodulating innoculant.
A plant species of the family FABACEAE widely cultivated for ANIMAL FEED.
The relationship between two different species of organisms that are interdependent; each gains benefits from the other or a relationship between different species where both of the organisms in question benefit from the presence of the other.
The process in certain BACTERIA; FUNGI; and CYANOBACTERIA converting free atmospheric NITROGEN to biologically usable forms of nitrogen, such as AMMONIA; NITRATES; and amino compounds.
The formation of a nitrogen-fixing cell mass on PLANT ROOTS following symbiotic infection by nitrogen-fixing bacteria such as RHIZOBIUM or FRANKIA.
A plant genus of the family FABACEAE. It is distinct from Sweet Clover (MELILOTUS), from Bush Clover (LESPEDEZA), and from Red Clover (TRIFOLIUM).
A plant species of the family FABACEAE used to study GENETICS because it is DIPLOID, self fertile, has a small genome, and short generation time.
A family of gram-negative bacteria which are saprophytes, symbionts, or plant pathogens.
Knobbed structures formed from and attached to plant roots, especially of LEGUMES, which result from symbiotic infection by nitrogen fixing bacteria such as RHIZOBIUM or FRANKIA. Root nodules are structures related to MYCORRHIZAE formed by symbiotic associations with fungi.
Proteins found in any species of bacterium.
Any of the processes by which cytoplasmic or intercellular factors influence the differential control of gene action in bacteria.
The large family of plants characterized by pods. Some are edible and some cause LATHYRISM or FAVISM and other forms of poisoning. Other species yield useful materials like gums from ACACIA and various LECTINS like PHYTOHEMAGGLUTININS from PHASEOLUS. Many of them harbor NITROGEN FIXATION bacteria on their roots. Many but not all species of "beans" belong to this family.
The usually underground portions of a plant that serve as support, store food, and through which water and mineral nutrients enter the plant. (From American Heritage Dictionary, 1982; Concise Dictionary of Biology, 1990)
A genus of gram-negative, aerobic, rod-shaped bacteria that activate PLANT ROOT NODULATION in leguminous plants. Members of this genus are nitrogen-fixing and common soil inhabitants.
Polysaccharides found in bacteria and in capsules thereof.
The functional hereditary units of BACTERIA.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
A plant genus of the family FABACEAE.
A species of gram-negative, aerobic bacteria that is found in soil and which causes formation of root nodules on some, but not all, types of field pea, lentil, kidney bean, and clover.
A genus of gram-negative, aerobic, rod-shaped bacteria usually containing granules of poly-beta-hydroxybutyrate. They characteristically invade the root hairs of leguminous plants and act as intracellular symbionts.
5,7,3',4'-tetrahydroxy-flavone, one of the FLAVONES.
A plant genus of the family FABACEAE. Members contain piperidine alkaloids (PIPERIDINES).
Deoxyribonucleic acid that makes up the genetic material of bacteria.
A plant genus of the family FABACEAE. The common name of indigo also refers to Baptisia or Amorpha genera (FABACEAE).
An annual legume. The SEEDS of this plant are edible and used to produce a variety of SOY FOODS.
Mutagenesis where the mutation is caused by the introduction of foreign DNA sequences into a gene or extragenic sequence. This may occur spontaneously in vivo or be experimentally induced in vivo or in vitro. Proviral DNA insertions into or adjacent to a cellular proto-oncogene can interrupt GENETIC TRANSLATION of the coding sequences or interfere with recognition of regulatory elements and cause unregulated expression of the proto-oncogene resulting in tumor formation.
A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.
The genetic complement of a BACTERIA as represented in its DNA.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
A test used to determine whether or not complementation (compensation in the form of dominance) will occur in a cell with a given mutant phenotype when another mutant genome, encoding the same mutant phenotype, is introduced into that cell.
One of the FURANS with a carbonyl thereby forming a cyclic lactone. It is an endogenous compound made from gamma-aminobutyrate and is the precursor of gamma-hydroxybutyrate. It is also used as a pharmacological agent and solvent.
Polysaccharides composed of repeating galactose units. They can consist of branched or unbranched chains in any linkages.
A species of gram-negative, aerobic bacteria isolated from soil and the stems, leafs, and roots of plants. Some biotypes are pathogenic and cause the formation of PLANT TUMORS in a wide variety of higher plants. The species is a major research tool in biotechnology.
A mononuclear Fe(II)-dependent oxygenase, this enzyme catalyzes the conversion of homogentisate to 4-maleylacetoacetate, the third step in the pathway for the catabolism of TYROSINE. Deficiency in the enzyme causes ALKAPTONURIA, an autosomal recessive disorder, characterized by homogentisic aciduria, OCHRONOSIS and ARTHRITIS. This enzyme was formerly characterized as EC 1.13.1.5 and EC 1.99.2.5.
A plant genus of the family FABACEAE. The gums and tanning agents obtained from Acacia are called GUM ARABIC. The common name of catechu is more often used for Areca catechu (ARECA).
In bacteria, a group of metabolically related genes, with a common promoter, whose transcription into a single polycistronic MESSENGER RNA is under the control of an OPERATOR REGION.
A phenomenon where microorganisms communicate and coordinate their behavior by the accumulation of signaling molecules. A reaction occurs when a substance accumulates to a sufficient concentration. This is most commonly seen in bacteria.
The relationships of groups of organisms as reflected by their genetic makeup.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
A naturally occurring compound that has been of interest for its role in osmoregulation. As a drug, betaine hydrochloride has been used as a source of hydrochloric acid in the treatment of hypochlorhydria. Betaine has also been used in the treatment of liver disorders, for hyperkalemia, for homocystinuria, and for gastrointestinal disturbances. (From Martindale, The Extra Pharmacopoeia, 30th ed, p1341)
A class in the phylum PROTEOBACTERIA comprised mostly of two major phenotypes: purple non-sulfur bacteria and aerobic bacteriochlorophyll-containing bacteria.
A species in the genus ANABAENA whose trichomes are composed of cylindrical cells.
The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment.
An enzyme bound to the inner mitochondrial membrane that catalyzes the oxidation of CHOLINE to BETAINE.
In eukaryotes, a genetic unit consisting of a noncontiguous group of genes under the control of a single regulator gene. In bacteria, regulons are global regulatory systems involved in the interplay of pleiotropic regulatory domains and consist of several OPERONS.
Discrete segments of DNA which can excise and reintegrate to another site in the genome. Most are inactive, i.e., have not been found to exist outside the integrated state. DNA transposable elements include bacterial IS (insertion sequence) elements, Tn elements, the maize controlling elements Ac and Ds, Drosophila P, gypsy, and pogo elements, the human Tigger elements and the Tc and mariner elements which are found throughout the animal kingdom.
Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.
Polysaccharides composed of repeating glucose units. They can consist of branched or unbranched chains in any linkages.
Constituent of 30S subunit prokaryotic ribosomes containing 1600 nucleotides and 21 proteins. 16S rRNA is involved in initiation of polypeptide synthesis.
Cyclic esters of acylated BUTYRIC ACID containing four carbons in the ring.
Plants whose roots, leaves, seeds, bark, or other constituent parts possess therapeutic, tonic, purgative, curative or other pharmacologic attributes, when administered to man or animals.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
A family of organic anion transporters that specifically transport DICARBOXYLIC ACIDS such as alpha-ketoglutaric acid across cellular membranes.
The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.
The presence of bacteria, viruses, and fungi in the soil. This term is not restricted to pathogenic organisms.
A species of gram-negative bacteria and an nitrogen inoculum that displays a high intrinsic tolerance to acidity.
A plant genus in the family FABACEAE which is the source of edible beans and the lectin PHYTOHEMAGGLUTININS.
An NAD+ dependent enzyme that catalyzes the oxidation of betain aldehyde to BETAINE.
Ribonucleic acid in bacteria having regulatory and catalytic roles as well as involvement in protein synthesis.
A species of gram-negative bacteria and nitrogen innoculant of PHASEOLUS VULGARIS.
An integration host factor that was originally identified as a bacterial protein required for the integration of bacteriophage Q beta (ALLOLEVIVIRUS). Its cellular function may be to regulate mRNA stability and processing in that it binds tightly to poly(A) RNA and interferes with ribosome binding.
An element with the atomic symbol N, atomic number 7, and atomic weight [14.00643; 14.00728]. Nitrogen exists as a diatomic gas and makes up about 78% of the earth's atmosphere by volume. It is a constituent of proteins and nucleic acids and found in all living cells.
Those genes found in an organism which are necessary for its viability and normal function.
A four-carbon sugar that is found in algae, fungi, and lichens. It is twice as sweet as sucrose and can be used as a coronary vasodilator.
Any DNA sequence capable of independent replication or a molecule that possesses a REPLICATION ORIGIN and which is therefore potentially capable of being replicated in a suitable cell. (Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
The in vitro fusion of GENES by RECOMBINANT DNA techniques to analyze protein behavior or GENE EXPRESSION REGULATION, or to merge protein functions for specific medical or industrial uses.
Salts and esters of hydroxybutyric acid.
A transfer RNA which is specific for carrying arginine to sites on the ribosomes in preparation for protein synthesis.
A plant species of the family FABACEAE.
A genetic rearrangement through loss of segments of DNA or RNA, bringing sequences which are normally separated into close proximity. This deletion may be detected using cytogenetic techniques and can also be inferred from the phenotype, indicating a deletion at one specific locus.
A genus of filamentous CYANOBACTERIA in the order Oscillatoriales. It is commonly found in freshwater environments, especially hot springs.
An organophosphorus compound isolated from human and animal tissues.
Dicarboxylic acids are organic compounds containing two carboxyl (-COOH) groups in their structure, making them capable of forming salts and esters by losing two hydrogen ions.
Polymers of organic acids and alcohols, with ester linkages--usually polyethylene terephthalate; can be cured into hard plastic, films or tapes, or fibers which can be woven into fabrics, meshes or velours.
The sequential location of genes on a chromosome.
3'-Phosphoadenosine-5'-phosphosulfate. Key intermediate in the formation by living cells of sulfate esters of phenols, alcohols, steroids, sulfated polysaccharides, and simple esters, such as choline sulfate. It is formed from sulfate ion and ATP in a two-step process. This compound also is an important step in the process of sulfur fixation in plants and microorganisms.
Consists of a polypeptide chain and 4'-phosphopantetheine linked to a serine residue by a phosphodiester bond. Acyl groups are bound as thiol esters to the pantothenyl group. Acyl carrier protein is involved in every step of fatty acid synthesis by the cytoplasmic system.
Structures within the nucleus of bacterial cells consisting of or containing DNA, which carry genetic information essential to the cell.
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.

Structural determination of a 5-acetamido-3,5,7, 9-tetradeoxy-7-(3-hydroxybutyramido)-L-glycero-L-manno-nonulos onic acid-containing homopolysaccharide isolated from Sinorhizobium fredii HH103. (1/68)

The structure of a polysaccharide from Sinorhizobium fredii HH103 has been determined. This polysaccharide was isolated by following the protocol for lipopolysaccharide extraction. On the basis of monosaccharide analysis, methylation analysis, fast atom bombardment MS, matrix-assisted laser desorption ionization MS, electron-impact high-resolution MS, one-dimensional (1)H-NMR and (13)C-NMR and two-dimensional NMR experiments, the structure was shown to consist of a homopolymer of a 3:1 mixture of 5-acetamido-3,5,7, 9-tetradeoxy-7-[(R)- and (S)-3-hydroxybutyramido]-l-glycero-l-manno-nonulosonic acid. The sugar residues are attached via a glycosidic linkage to the OH group of the 3-hydroxybutyramido substituent and thus the monomers are linked via both glycosidic and amidic linkages. In contrast with the Sinorhizobium K-antigens previously reported, which are composed of a disaccharide repeating unit, the K-antigen polysacharide of S. fredii HH103 is a homopolysaccharide.  (+info)

Inhibition of the FixL sensor kinase by the FixT protein in Sinorhizobium meliloti. (2/68)

Nitrogen fixation in symbiotic rhizobia is subject to multiple levels of gene regulation. In Sinorhizobium meliloti, the alfalfa symbiont, the FixLJ two-component regulatory system plays a major role in inducing nitrogen fixation and respiration gene expression in response to the low ambient O(2) concentration of the nodule. Here we report on the mode of action of the FixT protein, a recently identified repressor of nitrogen fixation gene expression in S. meliloti. First, we provide evidence that FixT prevents transcription of the intermediate key regulatory genes nifA and fixK by counteracting the activity of the FixLJ two-component system under otherwise inducing microoxic conditions. Second, we demonstrate that FixT acts as an inhibitor of the sensor hemoprotein kinase FixL, preventing the production or the accumulation of its phosphorylated form. FixT is thus a new example of a regulatory protein that blocks signal transduction in two-component systems at the level of the sensor kinase.  (+info)

Sinorhizobium arboris sp. nov. and Sinorhizobium kostiense sp. nov., isolated from leguminous trees in Sudan and Kenya. (3/68)

SDS-PAGE of total bacterial proteins was applied to the classification of 25 Sudanese and five Kenyan strains isolated from the root nodules of Acacia senegal and Prosopis chilensis. Twenty strains were also studied by multilocus enzyme electrophoresis (MLEE) and the whole 16S rRNA gene was sequenced from two strains representing the two major clusters. These results, together with the previously reported numerical taxonomy analysis, pulsed-field gel electrophoresis studies, DNA-DNA dot-blot hybridization, genomic fingerprinting using repetitive sequence-based PCR, DNA base composition analysis, DNA-DNA reassociation analysis, partial sequencing of the 16S rRNA gene and RFLP analysis of the amplified 16S rRNA gene, showed that all 30 strains belong to the genus Sinorhizobium. Two of the strains grouped with Sinorhizobium saheli and seven with Sinorhizobium terangae, while the rest did not cluster with any of the established species. The majority of the strains formed two phenotypically and genotypically distinct groups and we therefore propose that these strains should be classified as two new species, Sinorhizobium arboris sp. nov. and Sinorhizobium kostiense sp. nov.  (+info)

Sinorhizobium fredii USDA257, a cultivar-specific soybean symbiont, carries two copies of y4yA and y4yB, two open reading frames that are located in a region that encodes the type III protein secretion system. (4/68)

Sinorhizobium fredii USDA257 forms nitrogen-fixing nodules on primitive soybean (Glycine max) cultivar Peking but fails to nodulate the improved cultivar McCall. Cultivar specificity is governed by a plasmid-borne locus, nolXBTUV. By DNA sequence analysis, we have identified two open reading frames, y4yA and y4yB, immediately downstream of nolX. Northern (RNA) blot analysis indicated that the expression of both y4yA and y4yB is inducible by isoflavonoids, and an intact copy of nolX is required. Two copies each of y4yA and y4yB are present in S. fredii USDA257, one on the sym plasmid (y4yAsp and y4yBsp), and the other on the chromosome (y4yAc and y4yBc). The cultivar-nonspecific strain USDA191 lacks y4yAc and y4yBc. Introduction of y4yAc plus y4yBc from USDA257 into USDA191 did not influence the ability of the latter strain to nodulate McCall soybean plants. Unlike nolX, the inactivation of y4yAsp and y4yBsp of USDA257 did not extend the host range of this strain. A double mutant, in which both the plasmid and chromosomal copies of y4yA and y4yB were mutated, had no observable effect on symbiotic ability of USDA257. The y4yAsp and y4yBsp mutants did not influence flavonoid-dependent extracellular protein production. Rhizobium sp. strain NGR234 and S. saheli USDA4893 both contain sequences similar to S. fredii USDA257 y4yAsp and y4yBsp; however, Bradyrhizobium spp., the traditional soybean symbionts, lack these genes.  (+info)

Characterization of rhizobial isolates of Phaseolus vulgaris by staircase electrophoresis of low-molecular-weight RNA. (5/68)

Low-molecular-weight (LMW) RNA molecules were analyzed to characterize rhizobial isolates that nodulate the common bean growing in Spain. Since LMW RNA profiles, determined by staircase electrophoresis, varied across the rhizobial species nodulating beans, we demonstrated that bean isolates recovered from Spanish soils presumptively could be characterized as Rhizobium etli, Rhizobium gallicum, Rhizobium giardinii, Rhizobium leguminosarum bv. viciae and bv. trifolii, and Sinorhizobium fredii.  (+info)

A functional myo-inositol dehydrogenase gene is required for efficient nitrogen fixation and competitiveness of Sinorhizobium fredii USDA191 to nodulate soybean (Glycine max [L.] Merr.). (6/68)

Inositol derivative compounds provide a nutrient source for soil bacteria that possess the ability to degrade such compounds. Rhizobium strains that are capable of utilizing certain inositol derivatives are better colonizers of their host plants. We have cloned and determined the nucleotide sequence of the myo-inositol dehydrogenase gene (idhA) of Sinorhizobium fredii USDA191, the first enzyme responsible for inositol catabolism. The deduced IdhA protein has a molecular mass of 34,648 Da and shows significant sequence similarity with protein sequences of Sinorhizobium meliloti IdhA and MocA; Bacillus subtilis IolG, YrbE, and YucG; and Streptomyces griseus StrI. S. fredii USDA191 idhA mutants revealed no detectable myo-inositol dehydrogenase activity and failed to grow on myo-inositol as a sole carbon source. Northern blot analysis and idhA-lacZ fusion expression studies indicate that idhA is inducible by myo-inositol. S. fredii USDA191 idhA mutant was drastically affected in its ability to reduce nitrogen and revealed deteriorating bacteroids inside the nodules. The number of bacteria recovered from such nodules was about threefold lower than the number of bacteria isolated from nodules initiated by S. fredii USDA191. In addition, the idhA mutant was also severely affected in its ability to compete with the wild-type strain in nodulating soybean. Under competitive conditions, nodules induced on soybean roots were predominantly occupied by the parent strain, even when the idhA mutant was applied at a 10-fold numerical advantage. Thus, we conclude that a functional idhA gene is required for efficient nitrogen fixation and for competitive nodulation of soybeans by S. fredii USDA191.  (+info)

Classification of rhizobia based on nodC and nifH gene analysis reveals a close phylogenetic relationship among Phaseolus vulgaris symbionts. (7/68)

The nodC and nifH genes were characterized in a collection of 83 rhizobial strains which represented 23 recognized species distributed in the genera Rhizobium, Sinorhizobium, Mesorhizobium and Bradyrhizobium, as well as unclassified rhizobia from various host legumes. Conserved primers were designed from available nucleotide sequences and were able to amplify nodC and nifH fragments of about 930 bp and 780 bp, respectively, from most of the strains investigated. RFLP analysis of the PCR products resulted in a classification of these rhizobia which was in general well-correlated with their known host range and independent of their taxonomic status. The nodC and nifH fragments were sequenced for representative strains belonging to different genera and species, most of which originated from Phaselous vulgaris nodules. Phylogenetic trees were constructed and revealed close relationships among symbiotic genes of the Phaseolus symbionts, irrespective of their 16S-rDNA-based classification. The nodC and nifH phylogenies were generally similar, but cases of incongruence were detected, suggesting that genetic rearrangements have occurred in the course of evolution. The results support the view that lateral genetic transfer across rhizobial species and, in some instances, across Rhizobium and Sinorhizobium genera plays a role in diversification and in structuring the natural populations of rhizobia.  (+info)

Differential regulation of a family of apyrase genes from Medicago truncatula. (8/68)

Four putative apyrase genes were identified from the model legume Medicago truncatula. Two of the genes identified from M. truncatula (Mtapy1 and Mtapy4) are expressed in roots and are inducible within 3 h after inoculation with Sinorhizobium meliloti. The level of mRNA expression of the other two putative apyrases, Mtapy2 and Mtapy3, was unaffected by rhizobial inoculation. Screening of a bacterial artificial chromosome library of M. truncatula genomic DNA showed that Mtapy1, Mtapy3, and Mtapy4 are present on a single bacterial artificial chromosome clone. This apyrase cluster was mapped to linkage group seven. A syntenic region on soybean linkage group J was found to contain at least two apyrase genes. Screening of nodulation deficient mutants of M. truncatula revealed that two such mutants do not express apyrases to any detectable level. The data suggest a role for apyrases early in the nodulation response before the involvement of root cortical cell division leading to the nodule structure.  (+info)

"Sinorhizobium meliloti" is a species of nitrogen-fixing bacteria that forms nodules on the roots of leguminous plants, such as alfalfa and clover. These bacteria have the ability to convert atmospheric nitrogen into ammonia, which can then be used by the plant for growth and development. This symbiotic relationship benefits both the bacterium and the plant, as the plant provides carbon sources to the bacterium, while the bacterium provides the plant with a source of nitrogen.

"Sinorhizobium meliloti" is gram-negative, motile, and rod-shaped, and it can be found in soil and root nodules of leguminous plants. It has a complex genome consisting of a circular chromosome and several plasmids, which carry genes involved in nitrogen fixation and other important functions. The bacteria are able to sense and respond to various environmental signals, allowing them to adapt to changing conditions and establish successful symbioses with their host plants.

In addition to its agricultural importance, "Sinorhizobium meliloti" is also a model organism for studying the molecular mechanisms of symbiotic nitrogen fixation and bacterial genetics.

"Sinorhizobium" is a genus of bacteria that can form nitrogen-fixing nodules on the roots of certain leguminous plants, such as beans and alfalfa. These bacteria are able to convert atmospheric nitrogen into ammonia, which the plant can then use for growth. This symbiotic relationship benefits both the plant and the bacteria - the plant receives a source of nitrogen, while the bacteria receive carbon and other nutrients from the plant.

The genus "Sinorhizobium" is part of the family Rhizobiaceae and includes several species that are important for agriculture and the global nitrogen cycle. Some examples of "Sinorhizobium" species include S. meliloti, which forms nodules on alfalfa and other Medicago species, and S. fredii, which forms nodules on soybeans and other Glycine species.

It's worth noting that the taxonomy of nitrogen-fixing bacteria has undergone significant revisions in recent years, and some "Sinorhizobium" species have been reclassified as members of other genera. However, the genus "Sinorhizobium" remains a valid and important group of nitrogen-fixing bacteria.

"Sinorhizobium fredii" is a gram-negative, rod-shaped bacterium that belongs to the family Rhizobiaceae. It has the ability to fix atmospheric nitrogen in a symbiotic relationship with certain leguminous plants, particularly soybeans and other related species. This bacterium infects the roots of these plants and forms nodules where it converts nitrogen gas into ammonia, a form that can be used by the plant for growth.

"Sinorhizobium fredii" is widely distributed in soil and is known to have a broad host range, including many important agricultural crops. It has been studied extensively due to its potential use as a biofertilizer, which could help reduce the need for chemical nitrogen fertilizers and improve soil health.

It's worth noting that while "Sinorhizobium fredii" is an important bacterium in agriculture and environmental science, it can also be a pathogen of some plants under certain conditions. Therefore, understanding its biology and ecology is crucial for optimizing its benefits and minimizing any potential negative impacts.

'Medicago sativa' is the scientific name for a plant species more commonly known as alfalfa. In a medical context, alfalfa is often considered a herbal supplement and its medicinal properties include being a source of vitamins, minerals, and antioxidants. It has been used in traditional medicine to treat a variety of conditions such as kidney problems, asthma, arthritis, and high cholesterol levels. However, it's important to note that the effectiveness of alfalfa for these uses is not conclusively established by scientific research and its use may have potential risks or interactions with certain medications. Always consult a healthcare provider before starting any new supplement regimen.

In the context of medicine and biology, symbiosis is a type of close and long-term biological interaction between two different biological organisms. Generally, one organism, called the symbiont, lives inside or on another organism, called the host. This interaction can be mutually beneficial (mutualistic), harmful to the host organism (parasitic), or have no effect on either organism (commensal).

Examples of mutualistic symbiotic relationships in humans include the bacteria that live in our gut and help us digest food, as well as the algae that live inside corals and provide them with nutrients. Parasitic symbioses, on the other hand, involve organisms like viruses or parasitic worms that live inside a host and cause harm to it.

It's worth noting that while the term "symbiosis" is often used in popular culture to refer to any close relationship between two organisms, in scientific contexts it has a more specific meaning related to long-term biological interactions.

Nitrogen fixation is a process by which nitrogen gas (N2) in the air is converted into ammonia (NH3) or other chemically reactive forms, making it available to plants and other organisms for use as a nutrient. This process is essential for the nitrogen cycle and for the growth of many types of plants, as most plants cannot utilize nitrogen gas directly from the air.

In the medical field, nitrogen fixation is not a commonly used term. However, in the context of microbiology and infectious diseases, some bacteria are capable of fixing nitrogen and this ability can contribute to their pathogenicity. For example, certain species of bacteria that colonize the human body, such as those found in the gut or on the skin, may be able to fix nitrogen and use it for their own growth and survival. In some cases, these bacteria may also release fixed nitrogen into the environment, which can have implications for the ecology and health of the host and surrounding ecosystems.

Plant root nodulation is a type of symbiotic relationship between certain plants (mostly legumes) and nitrogen-fixing bacteria, such as Rhizobia species. This process involves the formation of specialized structures called nodules on the roots of the host plant. The bacteria inhabit these nodules and convert atmospheric nitrogen into ammonia, a form of nitrogen that plants can use for growth. In return, the plant provides the bacteria with carbon sources and a protected environment for growth. This mutualistic relationship helps improve soil fertility and promotes sustainable agriculture.

"Medicago" is a genus of flowering plants in the family Fabaceae, also known as the legume or pea family. It includes several species that are important forage crops and green manure, such as Medicago sativa (alfalfa or lucerne) and Medicago lupulina (black medic). These plants have the ability to fix nitrogen from the atmosphere through their root nodules, which benefits the soil and other nearby plants. They are often used in rotational grazing systems and for erosion control.

'Medicago truncatula' is not a medical term, but a scientific name for a plant species. It is commonly known as barrel medic or yellow trefoil and is native to the Mediterranean region. It is a model organism in the field of plant genetics and molecular biology due to its small genome size and ease of transformation. While it does not have direct medical applications, studies on this plant can contribute to our understanding of fundamental biological processes and may have indirect implications for human health.

Rhizobiaceae is a family of bacteria that have the ability to fix nitrogen. These bacteria are gram-negative, motile, and rod-shaped. They are commonly found in the root nodules of leguminous plants, where they form a symbiotic relationship with the plant. The bacteria provide the plant with fixed nitrogen, while the plant provides the bacteria with carbon and a protected environment.

The most well-known genus of Rhizobiaceae is Rhizobium, which includes several species that are important for agriculture because of their ability to fix nitrogen in the root nodules of legumes. Other genera in this family include Bradyrhizobium, Mesorhizobium, and Sinorhizobium.

It's worth noting that while Rhizobiaceae bacteria are generally beneficial, they can sometimes cause disease in plants under certain conditions. For example, some strains of Rhizobium can cause leaf spots on certain crops.

Root nodules in plants refer to the specialized structures formed through the symbiotic relationship between certain leguminous plants and nitrogen-fixing bacteria, most commonly belonging to the genus Rhizobia. These nodules typically develop on the roots of the host plant, providing an ideal environment for the bacteria to convert atmospheric nitrogen into ammonia, a form that can be directly utilized by the plant for growth and development.

The formation of root nodules begins with the infection of the plant's root hair cells by Rhizobia bacteria. This interaction triggers a series of molecular signals leading to the differentiation of root cortical cells into nodule primordia, which eventually develop into mature nodules. The nitrogen-fixing bacteria reside within these nodules in membrane-bound compartments called symbiosomes, where they reduce atmospheric nitrogen into ammonia through an enzyme called nitrogenase.

The plant, in turn, provides the bacteria with carbon sources and other essential nutrients required for their growth and survival within the nodules. The fixed nitrogen is then transported from the root nodules to other parts of the plant, enhancing its overall nitrogen nutrition and promoting sustainable growth without the need for external nitrogen fertilizers.

In summary, root nodules in plants are essential structures formed through symbiotic associations with nitrogen-fixing bacteria, allowing leguminous plants to convert atmospheric nitrogen into a usable form while also benefiting the environment by reducing the reliance on chemical nitrogen fertilizers.

Bacterial proteins are a type of protein that are produced by bacteria as part of their structural or functional components. These proteins can be involved in various cellular processes, such as metabolism, DNA replication, transcription, and translation. They can also play a role in bacterial pathogenesis, helping the bacteria to evade the host's immune system, acquire nutrients, and multiply within the host.

Bacterial proteins can be classified into different categories based on their function, such as:

1. Enzymes: Proteins that catalyze chemical reactions in the bacterial cell.
2. Structural proteins: Proteins that provide structural support and maintain the shape of the bacterial cell.
3. Signaling proteins: Proteins that help bacteria to communicate with each other and coordinate their behavior.
4. Transport proteins: Proteins that facilitate the movement of molecules across the bacterial cell membrane.
5. Toxins: Proteins that are produced by pathogenic bacteria to damage host cells and promote infection.
6. Surface proteins: Proteins that are located on the surface of the bacterial cell and interact with the environment or host cells.

Understanding the structure and function of bacterial proteins is important for developing new antibiotics, vaccines, and other therapeutic strategies to combat bacterial infections.

Gene expression regulation in bacteria refers to the complex cellular processes that control the production of proteins from specific genes. This regulation allows bacteria to adapt to changing environmental conditions and ensure the appropriate amount of protein is produced at the right time.

Bacteria have a variety of mechanisms for regulating gene expression, including:

1. Operon structure: Many bacterial genes are organized into operons, which are clusters of genes that are transcribed together as a single mRNA molecule. The expression of these genes can be coordinately regulated by controlling the transcription of the entire operon.
2. Promoter regulation: Transcription is initiated at promoter regions upstream of the gene or operon. Bacteria have regulatory proteins called sigma factors that bind to the promoter and recruit RNA polymerase, the enzyme responsible for transcribing DNA into RNA. The binding of sigma factors can be influenced by environmental signals, allowing for regulation of transcription.
3. Attenuation: Some operons have regulatory regions called attenuators that control transcription termination. These regions contain hairpin structures that can form in the mRNA and cause transcription to stop prematurely. The formation of these hairpins is influenced by the concentration of specific metabolites, allowing for regulation of gene expression based on the availability of those metabolites.
4. Riboswitches: Some bacterial mRNAs contain regulatory elements called riboswitches that bind small molecules directly. When a small molecule binds to the riboswitch, it changes conformation and affects transcription or translation of the associated gene.
5. CRISPR-Cas systems: Bacteria use CRISPR-Cas systems for adaptive immunity against viruses and plasmids. These systems incorporate short sequences from foreign DNA into their own genome, which can then be used to recognize and cleave similar sequences in invading genetic elements.

Overall, gene expression regulation in bacteria is a complex process that allows them to respond quickly and efficiently to changing environmental conditions. Understanding these regulatory mechanisms can provide insights into bacterial physiology and help inform strategies for controlling bacterial growth and behavior.

Fabaceae is the scientific name for a family of flowering plants commonly known as the legume, pea, or bean family. This family includes a wide variety of plants that are important economically, agriculturally, and ecologically. Many members of Fabaceae have compound leaves and produce fruits that are legumes, which are long, thin pods that contain seeds. Some well-known examples of plants in this family include beans, peas, lentils, peanuts, clover, and alfalfa.

In addition to their importance as food crops, many Fabaceae species have the ability to fix nitrogen from the atmosphere into the soil through a symbiotic relationship with bacteria that live in nodules on their roots. This makes them valuable for improving soil fertility and is one reason why they are often used in crop rotation and as cover crops.

It's worth noting that Fabaceae is sometimes still referred to by its older scientific name, Leguminosae.

A plant root is not a medical term per se, but it is a term from botany, which is the study of plants. However, I can certainly provide a definition for you.

Plant roots are the underground organs of a plant that typically grow downward into the soil. They serve several important functions, including:

1. Anchorage: Roots help to stabilize the plant and keep it upright in the ground.
2. Absorption: Roots absorb water and nutrients from the soil, which are essential for the plant's growth and development.
3. Conduction: Roots conduct water and nutrients up to the above-ground parts of the plant, such as the stem and leaves.
4. Vegetative reproduction: Some plants can reproduce vegetatively through their roots, producing new plants from root fragments or specialized structures called rhizomes or tubers.

Roots are composed of several different tissues, including the epidermis, cortex, endodermis, and vascular tissue. The epidermis is the outermost layer of the root, which secretes a waxy substance called suberin that helps to prevent water loss. The cortex is the middle layer of the root, which contains cells that store carbohydrates and other nutrients. The endodermis is a thin layer of cells that surrounds the vascular tissue and regulates the movement of water and solutes into and out of the root. The vascular tissue consists of xylem and phloem, which transport water and nutrients throughout the plant.

Rhizobium is not a medical term, but rather a term used in microbiology and agriculture. It refers to a genus of gram-negative bacteria that can fix nitrogen from the atmosphere into ammonia, which can then be used by plants as a nutrient. These bacteria live in the root nodules of leguminous plants (such as beans, peas, and clover) and form a symbiotic relationship with them.

The host plant provides Rhizobium with carbon sources and a protected environment within the root nodule, while the bacteria provide the plant with fixed nitrogen. This mutualistic interaction plays a crucial role in maintaining soil fertility and promoting plant growth.

While Rhizobium itself is not directly related to human health or medicine, understanding its symbiotic relationship with plants can have implications for agricultural practices, sustainable farming, and global food security.

Bacterial polysaccharides are complex carbohydrates that consist of long chains of sugar molecules (monosaccharides) linked together by glycosidic bonds. They are produced and used by bacteria for various purposes such as:

1. Structural components: Bacterial polysaccharides, such as peptidoglycan and lipopolysaccharide (LPS), play a crucial role in maintaining the structural integrity of bacterial cells. Peptidoglycan is a major component of the bacterial cell wall, while LPS forms the outer layer of the outer membrane in gram-negative bacteria.
2. Nutrient storage: Some bacteria synthesize and store polysaccharides as an energy reserve, similar to how plants store starch. These polysaccharides can be broken down and utilized by the bacterium when needed.
3. Virulence factors: Bacterial polysaccharides can also function as virulence factors, contributing to the pathogenesis of bacterial infections. For example, certain bacteria produce capsular polysaccharides (CPS) that surround and protect the bacterial cells from host immune defenses, allowing them to evade phagocytosis and persist within the host.
4. Adhesins: Some polysaccharides act as adhesins, facilitating the attachment of bacteria to surfaces or host cells. This is important for biofilm formation, which helps bacteria resist environmental stresses and antibiotic treatments.
5. Antigenic properties: Bacterial polysaccharides can be highly antigenic, eliciting an immune response in the host. The antigenicity of these molecules can vary between different bacterial species or even strains within a species, making them useful as targets for vaccines and diagnostic tests.

In summary, bacterial polysaccharides are complex carbohydrates that serve various functions in bacteria, including structural support, nutrient storage, virulence factor production, adhesion, and antigenicity.

A bacterial gene is a segment of DNA (or RNA in some viruses) that contains the genetic information necessary for the synthesis of a functional bacterial protein or RNA molecule. These genes are responsible for encoding various characteristics and functions of bacteria such as metabolism, reproduction, and resistance to antibiotics. They can be transmitted between bacteria through horizontal gene transfer mechanisms like conjugation, transformation, and transduction. Bacterial genes are often organized into operons, which are clusters of genes that are transcribed together as a single mRNA molecule.

It's important to note that the term "bacterial gene" is used to describe genetic elements found in bacteria, but not all genetic elements in bacteria are considered genes. For example, some DNA sequences may not encode functional products and are therefore not considered genes. Additionally, some bacterial genes may be plasmid-borne or phage-borne, rather than being located on the bacterial chromosome.

Molecular sequence data refers to the specific arrangement of molecules, most commonly nucleotides in DNA or RNA, or amino acids in proteins, that make up a biological macromolecule. This data is generated through laboratory techniques such as sequencing, and provides information about the exact order of the constituent molecules. This data is crucial in various fields of biology, including genetics, evolution, and molecular biology, allowing for comparisons between different organisms, identification of genetic variations, and studies of gene function and regulation.

"Melilotus" is the genus name for a group of plants commonly known as sweet clover or melilot. These plants are part of the Fabaceae family, which includes legumes. While "Melilotus" itself is not a medical term, some of its species have been used in traditional medicine and may be mentioned in medical literature.

The two most common species are Melilotus officinalis (yellow sweet clover) and Melilotus albus (white sweet clover). The plants contain coumarin derivatives, which can have anticoagulant properties. However, it is essential to note that consuming large amounts of these plants or their extracts can lead to adverse effects, including bleeding disorders.

In a medical context, "Melilotus" may be mentioned in relation to its potential medicinal uses, side effects, or interactions with other medications. Still, it is not considered a standard pharmaceutical drug or a widely accepted medical treatment. Always consult a healthcare professional before using any plant or herbal remedy for medicinal purposes.

'Rhizobium leguminosarum' is a species of bacteria that can form nitrogen-fixing nodules on the roots of certain leguminous plants, such as clover, peas, and beans. These bacteria have the ability to convert atmospheric nitrogen into ammonia, a form of nitrogen that plants can use for growth. This process, known as biological nitrogen fixation, benefits both the bacteria and the host plant, as the plant provides carbon sources to the bacteria, while the bacteria provide fixed nitrogen to the plant. The formation of this symbiotic relationship is facilitated by a molecular signaling process between the bacterium and the plant.

It's important to note that 'Rhizobium leguminosarum' is not a medical term per se, but rather a term used in microbiology, botany, and agriculture.

'Bradyrhizobium' is a genus of bacteria that can form nitrogen-fixing nodules on the roots of certain leguminous plants, such as soybeans and alfalfa. These bacteria are able to convert atmospheric nitrogen into ammonia, which the plant can then use for growth. This process, known as nitrogen fixation, is important for maintaining soil fertility and is beneficial for agricultural production.

The name 'Bradyrhizobium' comes from the Greek words "brady," meaning slow, and "rhiza," meaning root, reflecting the slower growth rate of these bacteria compared to other rhizobia. The bacteria are typically rod-shaped and motile, with a single polar flagellum for movement. They are gram-negative and have a complex cell envelope that includes an outer membrane, peptidoglycan layer, and cytoplasmic membrane.

Bradyrhizobium species are able to form symbiotic relationships with leguminous plants by colonizing the root nodules of the plant. The bacteria enter the plant through root hairs or wounds on the root surface, and then migrate to the inner cortex of the root where they induce the formation of nodules. Once inside the nodule, the bacteria differentiate into bacteroids that are able to fix nitrogen gas from the atmosphere into ammonia, which is then used by the plant for growth. In return, the plant provides carbon and other nutrients to the bacteria.

Bradyrhizobium species are important for sustainable agriculture because they can reduce the need for chemical fertilizers and improve soil health. They have also been studied for their potential use in bioremediation and as biofertilizers for non-leguminous crops.

Luteolin is a flavonoid, which is a type of plant pigment that has various beneficial effects on human health. It can be found in various fruits, vegetables, and herbs such as artichokes, peppers, celery, broccoli, peppermint, rosemary, and chamomile tea.

Luteolin has been shown to have anti-inflammatory, antioxidant, and anticancer properties in laboratory studies. It works by inhibiting the activity of certain enzymes and receptors that play a role in inflammation and cancer development. However, more research is needed to determine its effectiveness and safety as a treatment for various medical conditions.

I'm sorry for any confusion, but "Sesbania" is not a medical term. It is a genus of flowering plants in the pea and bean family Fabaceae, also known as "sesban" or "hummingbird tree." These plants have various uses, including as food sources for people and animals, as well as traditional medicinal applications in some cultures. However, there isn't a widely accepted medical definition associated with "Sesbania." If you have any questions related to health or medicine, I'd be happy to try to help with those!

Bacterial DNA refers to the genetic material found in bacteria. It is composed of a double-stranded helix containing four nucleotide bases - adenine (A), thymine (T), guanine (G), and cytosine (C) - that are linked together by phosphodiester bonds. The sequence of these bases in the DNA molecule carries the genetic information necessary for the growth, development, and reproduction of bacteria.

Bacterial DNA is circular in most bacterial species, although some have linear chromosomes. In addition to the main chromosome, many bacteria also contain small circular pieces of DNA called plasmids that can carry additional genes and provide resistance to antibiotics or other environmental stressors.

Unlike eukaryotic cells, which have their DNA enclosed within a nucleus, bacterial DNA is present in the cytoplasm of the cell, where it is in direct contact with the cell's metabolic machinery. This allows for rapid gene expression and regulation in response to changing environmental conditions.

I'm sorry for any confusion, but "Indigofera" is not a medical term. It is a genus of plants in the family Fabaceae, also known as the pea or legume family. The name "Indigofera" comes from the fact that some species of this plant are used to produce a blue dye called indigo.

While some plants in the Indigofera genus have been used in traditional medicine, there is no widely recognized medical definition specifically associated with Indigofera. If you have any questions related to a specific medical topic or condition, I would be happy to try and help answer those for you.

I'm sorry for any confusion, but "soybeans" are not a medical term. They are a type of legume that is commonly used in agriculture and food production. The medical community might discuss soybeans in the context of nutrition or allergies, but there isn't a formal medical definition for this term.

Here's some general information: Soybeans, scientifically known as Glycine max, are native to East Asia and are now grown worldwide. They are a significant source of plant-based protein and oil. Soybeans contain various nutrients, including essential amino acids, fiber, B vitamins, and minerals like calcium, iron, magnesium, and zinc. They are used in various food products such as tofu, soy milk, tempeh, and miso. Additionally, soybeans are also used in the production of industrial products, including biodiesel, plastics, and inks. Some people may have allergic reactions to soybeans or soy products.

Insertional mutagenesis is a process of introducing new genetic material into an organism's genome at a specific location, which can result in a change or disruption of the function of the gene at that site. This technique is often used in molecular biology research to study gene function and regulation. The introduction of the foreign DNA is typically accomplished through the use of mobile genetic elements, such as transposons or viruses, which are capable of inserting themselves into the genome.

The insertion of the new genetic material can lead to a loss or gain of function in the affected gene, resulting in a mutation. This type of mutagenesis is called "insertional" because the mutation is caused by the insertion of foreign DNA into the genome. The effects of insertional mutagenesis can range from subtle changes in gene expression to the complete inactivation of a gene.

This technique has been widely used in genetic research, including the study of developmental biology, cancer, and genetic diseases. It is also used in the development of genetically modified organisms (GMOs) for agricultural and industrial applications.

DNA Sequence Analysis is the systematic determination of the order of nucleotides in a DNA molecule. It is a critical component of modern molecular biology, genetics, and genetic engineering. The process involves determining the exact order of the four nucleotide bases - adenine (A), guanine (G), cytosine (C), and thymine (T) - in a DNA molecule or fragment. This information is used in various applications such as identifying gene mutations, studying evolutionary relationships, developing molecular markers for breeding, and diagnosing genetic diseases.

The process of DNA Sequence Analysis typically involves several steps, including DNA extraction, PCR amplification (if necessary), purification, sequencing reaction, and electrophoresis. The resulting data is then analyzed using specialized software to determine the exact sequence of nucleotides.

In recent years, high-throughput DNA sequencing technologies have revolutionized the field of genomics, enabling the rapid and cost-effective sequencing of entire genomes. This has led to an explosion of genomic data and new insights into the genetic basis of many diseases and traits.

A bacterial genome is the complete set of genetic material, including both DNA and RNA, found within a single bacterium. It contains all the hereditary information necessary for the bacterium to grow, reproduce, and survive in its environment. The bacterial genome typically includes circular chromosomes, as well as plasmids, which are smaller, circular DNA molecules that can carry additional genes. These genes encode various functional elements such as enzymes, structural proteins, and regulatory sequences that determine the bacterium's characteristics and behavior.

Bacterial genomes vary widely in size, ranging from around 130 kilobases (kb) in Mycoplasma genitalium to over 14 megabases (Mb) in Sorangium cellulosum. The complete sequencing and analysis of bacterial genomes have provided valuable insights into the biology, evolution, and pathogenicity of bacteria, enabling researchers to better understand their roles in various diseases and potential applications in biotechnology.

A mutation is a permanent change in the DNA sequence of an organism's genome. Mutations can occur spontaneously or be caused by environmental factors such as exposure to radiation, chemicals, or viruses. They may have various effects on the organism, ranging from benign to harmful, depending on where they occur and whether they alter the function of essential proteins. In some cases, mutations can increase an individual's susceptibility to certain diseases or disorders, while in others, they may confer a survival advantage. Mutations are the driving force behind evolution, as they introduce new genetic variability into populations, which can then be acted upon by natural selection.

A genetic complementation test is a laboratory procedure used in molecular genetics to determine whether two mutated genes can complement each other's function, indicating that they are located at different loci and represent separate alleles. This test involves introducing a normal or wild-type copy of one gene into a cell containing a mutant version of the same gene, and then observing whether the presence of the normal gene restores the normal function of the mutated gene. If the introduction of the normal gene results in the restoration of the normal phenotype, it suggests that the two genes are located at different loci and can complement each other's function. However, if the introduction of the normal gene does not restore the normal phenotype, it suggests that the two genes are located at the same locus and represent different alleles of the same gene. This test is commonly used to map genes and identify genetic interactions in a variety of organisms, including bacteria, yeast, and animals.

4-Butyrolactone, also known as gamma-butyrolactone (GBL) or 1,4-butanolide, is a chemical compound with the formula C4H6O2. It is a colorless oily liquid that is used in various industrial and commercial applications, including as an intermediate in the production of other chemicals, as a solvent, and as a flavoring agent.

In the medical field, 4-butyrolactone has been studied for its potential use as a sleep aid and muscle relaxant. However, it is not currently approved by regulatory agencies such as the US Food and Drug Administration (FDA) for these uses. It is also known to have abuse potential and can cause intoxication, sedation, and other central nervous system effects when ingested or inhaled.

It's important to note that 4-butyrolactone is not a medication and should only be used under the supervision of a qualified healthcare professional for approved medical purposes.

Galactans are a type of complex carbohydrates known as oligosaccharides that are composed of galactose molecules. They can be found in certain plants, including beans, lentils, and some fruits and vegetables. In the human body, galactans are not digestible and can reach the colon intact, where they may serve as a substrate for fermentation by gut bacteria. This can lead to the production of short-chain fatty acids, which have been shown to have various health benefits. However, in some individuals with irritable bowel syndrome or other functional gastrointestinal disorders, consumption of galactans may cause digestive symptoms such as bloating, gas, and diarrhea.

'Agrobacterium tumefaciens' is a gram-negative, soil-dwelling bacterium that is known for its ability to cause plant tumors or crown galls. It does this through the transfer and integration of a segment of DNA called the Ti (Tumor-inducing) plasmid into the plant's genome. This transferred DNA includes genes that encode enzymes for the production of opines, which serve as a nutrient source for the bacterium, and genes that cause unregulated plant cell growth leading to tumor formation.

This unique ability of 'Agrobacterium tumefaciens' to transfer and integrate foreign DNA into plants has been exploited in genetic engineering to create transgenic plants with desired traits. The Ti plasmid is often used as a vector to introduce new genes into the plant genome, making it an essential tool in plant biotechnology.

Homogentisate 1,2-dioxygenase (HGD) is an enzyme that plays a crucial role in the catabolism of tyrosine, an aromatic amino acid. This enzyme is involved in the third step of the tyrosine degradation pathway, also known as the tyrosine breakdown or catabolic pathway.

The homogentisate 1,2-dioxygenase enzyme catalyzes the conversion of homogentisic acid (HGA) into maleylacetoacetic acid. This reaction involves the cleavage of the aromatic ring of HGA and the introduction of oxygen, hence the name 'dioxygenase.' The reaction can be summarized as follows:

Homogentisate + O2 → Maleylacetoacetate

Deficiency or dysfunction in homogentisate 1,2-dioxygenase leads to a rare genetic disorder called alkaptonuria. In this condition, the body cannot break down tyrosine properly, resulting in an accumulation of HGA and its oxidation product, alkapton, which can cause damage to connective tissues and joints over time.

"Acacia" is a scientific name for a genus of shrubs and trees that belong to the pea family, Fabaceae. It includes over 1,350 species found primarily in Australia and Africa, but also in Asia, America, and Europe. Some acacia species are known for their hardwood, others for their phyllodes (flattened leaf stalks) or compound leaves, and yet others for their flowers, which are typically small and yellow or cream-colored.

It is important to note that "Acacia" is not a medical term or concept, but rather a botanical one. While some acacia species have medicinal uses, the name itself does not have a specific medical definition.

An operon is a genetic unit in prokaryotic organisms (like bacteria) consisting of a cluster of genes that are transcribed together as a single mRNA molecule, which then undergoes translation to produce multiple proteins. This genetic organization allows for the coordinated regulation of genes that are involved in the same metabolic pathway or functional process. The unit typically includes promoter and operator regions that control the transcription of the operon, as well as structural genes encoding the proteins. Operons were first discovered in bacteria, but similar genetic organizations have been found in some eukaryotic organisms, such as yeast.

Quorum sensing is a type of cell-cell communication that allows bacteria to detect and respond to changes in population density by producing, releasing, and responding to signaling molecules called autoinducers. This process enables the coordinated expression of certain genes related to various group behaviors such as biofilm formation, virulence factor production, and bioluminescence. The term "quorum sensing" was coined in 1994 by Bonnie L. Bassler and Susan Goldberg to describe this population-dependent gene regulation mechanism in bacteria.

Phylogeny is the evolutionary history and relationship among biological entities, such as species or genes, based on their shared characteristics. In other words, it refers to the branching pattern of evolution that shows how various organisms have descended from a common ancestor over time. Phylogenetic analysis involves constructing a tree-like diagram called a phylogenetic tree, which depicts the inferred evolutionary relationships among organisms or genes based on molecular sequence data or other types of characters. This information is crucial for understanding the diversity and distribution of life on Earth, as well as for studying the emergence and spread of diseases.

A base sequence in the context of molecular biology refers to the specific order of nucleotides in a DNA or RNA molecule. In DNA, these nucleotides are adenine (A), guanine (G), cytosine (C), and thymine (T). In RNA, uracil (U) takes the place of thymine. The base sequence contains genetic information that is transcribed into RNA and ultimately translated into proteins. It is the exact order of these bases that determines the genetic code and thus the function of the DNA or RNA molecule.

Betaine, also known as trimethylglycine, is a naturally occurring compound that can be found in various foods such as beets, spinach, and whole grains. In the body, betaine functions as an osmolyte, helping to regulate water balance in cells, and as a methyl donor, contributing to various metabolic processes including the conversion of homocysteine to methionine.

In medical terms, betaine is also used as a dietary supplement and medication. Betaine hydrochloride is a form of betaine that is sometimes used as a supplement to help with digestion by providing additional stomach acid. Betaine anhydrous, on the other hand, is often used as a supplement for improving athletic performance and promoting liver health.

Betaine has also been studied for its potential role in protecting against various diseases, including cardiovascular disease, diabetes, and neurological disorders. However, more research is needed to fully understand its mechanisms of action and therapeutic potential.

Alphaproteobacteria is a class of proteobacteria, a group of gram-negative bacteria. This class includes a diverse range of bacterial species that can be found in various environments, such as soil, water, and the surfaces of plants and animals. Some notable members of Alphaproteobacteria include the nitrogen-fixing bacteria Rhizobium and Bradyrhizobium, which form symbiotic relationships with the roots of leguminous plants, as well as the pathogenic bacteria Rickettsia, which are responsible for causing diseases such as typhus and Rocky Mountain spotted fever.

The Alphaproteobacteria class is further divided into several orders, including Rhizobiales, Rhodobacterales, and Caulobacterales. These orders contain a variety of bacterial species that have different characteristics and ecological roles. For example, members of the order Rhizobiales are known for their ability to fix nitrogen, while members of the order Rhodobacterales include photosynthetic bacteria that can use light as an energy source.

Overall, Alphaproteobacteria is a diverse and important group of bacteria that play various roles in the environment and in the health of plants and animals.

Anabaena cylindrica is a type of cyanobacteria, also known as blue-green algae. It forms filamentous colonies and can be found in various aquatic environments, such as freshwater and marine systems. This species is capable of fixing atmospheric nitrogen, making it an essential contributor to the nitrogen cycle in these ecosystems.

The term 'Anabaena cylindrica' refers to its morphological characteristics: 'Anabaena' comes from the Greek word 'anabainein', meaning 'to rise up', which alludes to the way the filaments float in the water column, and 'cylindrica' describes the cylindrical shape of the trichomes (filaments) formed by this species.

It is important to note that some strains of Anabaena cylindrica can produce harmful cyanotoxins, which may pose a risk to human health and aquatic ecosystems when blooms occur in bodies of water used for recreation or drinking water supply.

A phenotype is the physical or biochemical expression of an organism's genes, or the observable traits and characteristics resulting from the interaction of its genetic constitution (genotype) with environmental factors. These characteristics can include appearance, development, behavior, and resistance to disease, among others. Phenotypes can vary widely, even among individuals with identical genotypes, due to differences in environmental influences, gene expression, and genetic interactions.

Choline dehydrogenase is an enzyme that plays a role in the metabolism of choline, a nutrient that is essential for the normal functioning of cells. Specifically, choline dehydrogenase helps to catalyze the oxidation of choline to betaine aldehyde, which is then further metabolized to betaine. This reaction is an important step in the conversion of choline to a molecule called glycine betaine, which helps to regulate cell volume and protect cells from osmotic stress. Choline dehydrogenase is found in various tissues throughout the body, including the liver, kidneys, and brain. Deficiencies in choline or dysfunction of choline dehydrogenase can lead to a variety of health problems, including fatty liver disease, muscle damage, and neurological disorders.

A regulon is a group of genes that are regulated together in response to a specific signal or stimulus, often through the action of a single transcription factor or regulatory protein. This means that when the transcription factor binds to specific DNA sequences called operators, it can either activate or repress the transcription of all the genes within the regulon.

This type of gene regulation is important for coordinating complex biological processes, such as cellular metabolism, stress responses, and developmental programs. By regulating a group of genes together, cells can ensure that they are all turned on or off in a coordinated manner, allowing for more precise control over the overall response to a given signal.

It's worth noting that the term "regulon" is not commonly used in clinical medicine, but rather in molecular biology and genetics research.

DNA transposable elements, also known as transposons or jumping genes, are mobile genetic elements that can change their position within a genome. They are composed of DNA sequences that include genes encoding the enzymes required for their own movement (transposase) and regulatory elements. When activated, the transposase recognizes specific sequences at the ends of the element and catalyzes the excision and reintegration of the transposable element into a new location in the genome. This process can lead to genetic variation, as the insertion of a transposable element can disrupt the function of nearby genes or create new combinations of gene regulatory elements. Transposable elements are widespread in both prokaryotic and eukaryotic genomes and are thought to play a significant role in genome evolution.

A plasmid is a small, circular, double-stranded DNA molecule that is separate from the chromosomal DNA of a bacterium or other organism. Plasmids are typically not essential for the survival of the organism, but they can confer beneficial traits such as antibiotic resistance or the ability to degrade certain types of pollutants.

Plasmids are capable of replicating independently of the chromosomal DNA and can be transferred between bacteria through a process called conjugation. They often contain genes that provide resistance to antibiotics, heavy metals, and other environmental stressors. Plasmids have also been engineered for use in molecular biology as cloning vectors, allowing scientists to replicate and manipulate specific DNA sequences.

Plasmids are important tools in genetic engineering and biotechnology because they can be easily manipulated and transferred between organisms. They have been used to produce vaccines, diagnostic tests, and genetically modified organisms (GMOs) for various applications, including agriculture, medicine, and industry.

Glucans are polysaccharides (complex carbohydrates) that are made up of long chains of glucose molecules. They can be found in the cell walls of certain plants, fungi, and bacteria. In medicine, beta-glucans derived from yeast or mushrooms have been studied for their potential immune-enhancing effects. However, more research is needed to fully understand their role and effectiveness in human health.

Ribosomal RNA (rRNA) is a type of RNA that combines with proteins to form ribosomes, which are complex structures inside cells where protein synthesis occurs. The "16S" refers to the sedimentation coefficient of the rRNA molecule, which is a measure of its size and shape. In particular, 16S rRNA is a component of the smaller subunit of the prokaryotic ribosome (found in bacteria and archaea), and is often used as a molecular marker for identifying and classifying these organisms due to its relative stability and conservation among species. The sequence of 16S rRNA can be compared across different species to determine their evolutionary relationships and taxonomic positions.

Acyl-butyrolactones are a type of chemical compound that consists of a butyrolactone ring (a five-membered ring containing an oxygen atom and a carbonyl group) that has an acyl group (a functional group consisting of a carbon atom double-bonded to an oxygen atom and single-bonded to another functional group) attached to it.

Butyrolactones are lactones, which are cyclic esters derived from carboxylic acids. The addition of an acyl group to the butyrolactone ring results in the formation of acyl-butyrolactones. These compounds have a variety of uses in organic synthesis and may also be found in some natural sources.

It's worth noting that "acyl-butyrolactones" is a general term that can refer to any compound with this basic structure, and there may be many specific compounds that fall under this category. Therefore, it's important to consult a detailed chemical reference or speak with a chemist for more information on a specific acyl-butyrolactone compound.

Medicinal plants are defined as those plants that contain naturally occurring chemical compounds which can be used for therapeutic purposes, either directly or indirectly. These plants have been used for centuries in various traditional systems of medicine, such as Ayurveda, Chinese medicine, and Native American medicine, to prevent or treat various health conditions.

Medicinal plants contain a wide variety of bioactive compounds, including alkaloids, flavonoids, tannins, terpenes, and saponins, among others. These compounds have been found to possess various pharmacological properties, such as anti-inflammatory, analgesic, antimicrobial, antioxidant, and anticancer activities.

Medicinal plants can be used in various forms, including whole plant material, extracts, essential oils, and isolated compounds. They can be administered through different routes, such as oral, topical, or respiratory, depending on the desired therapeutic effect.

It is important to note that while medicinal plants have been used safely and effectively for centuries, they should be used with caution and under the guidance of a healthcare professional. Some medicinal plants can interact with prescription medications or have adverse effects if used inappropriately.

An amino acid sequence is the specific order of amino acids in a protein or peptide molecule, formed by the linking of the amino group (-NH2) of one amino acid to the carboxyl group (-COOH) of another amino acid through a peptide bond. The sequence is determined by the genetic code and is unique to each type of protein or peptide. It plays a crucial role in determining the three-dimensional structure and function of proteins.

Dicarboxylic acid transporters are a type of membrane transport protein that are responsible for the transportation of dicarboxylic acids across biological membranes. Dicarboxylic acids are organic compounds that contain two carboxyl groups, and they play important roles in various metabolic processes within the body.

The sodium-dependent dicarboxylic acid transporters (NaDCs) are a subfamily of these transporters that are widely expressed in many tissues, including the kidney, intestine, and brain. NaDCs mediate the uptake of dicarboxylates, such as succinate and glutarate, into cells in an energy-dependent manner, using the gradient of sodium ions across the membrane to drive the transport process.

The other subfamily of dicarboxylic acid transporters are the proton-coupled dicarboxylate transporters (PCDTs), which use a proton gradient to transport dicarboxylates. These transporters play important roles in the absorption and metabolism of dietary fibers, as well as in the regulation of intracellular pH.

Defects in dicarboxylic acid transporters have been implicated in several human diseases, including renal tubular acidosis, a condition characterized by impaired ability to excrete hydrogen ions and reabsorb bicarbonate ions in the kidney.

Sequence homology, amino acid, refers to the similarity in the order of amino acids in a protein or a portion of a protein between two or more species. This similarity can be used to infer evolutionary relationships and functional similarities between proteins. The higher the degree of sequence homology, the more likely it is that the proteins are related and have similar functions. Sequence homology can be determined through various methods such as pairwise alignment or multiple sequence alignment, which compare the sequences and calculate a score based on the number and type of matching amino acids.

I apologize, but there seems to be a misunderstanding. "Soil microbiology" is not a medical term; rather, it is a branch of microbiology that deals with the study of microorganisms in soil. It involves understanding the diversity, ecology, and biochemistry of bacteria, fungi, algae, protozoa, and other microscopic life forms found in soil. This field has applications in agriculture, environmental science, and waste management but is not directly related to medical definitions or human health.

Rhizobium tropici is a gram-negative, aerobic, motile, non-spore forming bacteria that belongs to the Rhizobiaceae family. It has the ability to fix atmospheric nitrogen in a symbiotic relationship with certain leguminous plants, particularly beans and other tropical legumes. The bacterium infects the roots of these plants and forms nodules where it converts nitrogen gas into ammonia, which is then used by the plant for growth. Rhizobium tropici is known for its ability to survive in a wide range of temperatures and soil conditions, making it an important contributor to sustainable agriculture in tropical regions.

"Phaseolus" is a term that refers to a genus of plants in the legume family Fabaceae, also known as the pea family. The most common and well-known species in this genus is "Phaseolus vulgaris," which is commonly called the common bean. This includes many familiar varieties such as kidney beans, black beans, navy beans, pinto beans, and green beans.

These plants are native to the Americas and have been cultivated for thousands of years for their edible seeds (beans) and pods (green beans). They are an important source of protein, fiber, vitamins, and minerals in many diets around the world.

It's worth noting that "Phaseolus" is a taxonomic term used in the scientific classification of plants, and it does not have a specific medical definition. However, the beans from these plants do have various health benefits and potential medicinal properties, such as being associated with reduced risk of heart disease, improved gut health, and better blood sugar control.

Betaine-aldehyde dehydrogenase (BADH) is an enzyme involved in the metabolic pathway of betaine, a compound that helps protect cells from environmental stress and is important for maintaining cell volume and osmotic balance. The enzyme catalyzes the conversion of betaine aldehyde to betaine, using NAD+ as a cofactor.

Deficiency in BADH has been associated with certain genetic disorders, such as hyperbetalipoproteinemia type I, which is characterized by elevated levels of lipids and lipoproteins in the blood. Additionally, mutations in the BADH gene have been linked to an increased risk of alcoholism and alcohol-related disorders.

Bacterial RNA refers to the genetic material present in bacteria that is composed of ribonucleic acid (RNA). Unlike higher organisms, bacteria contain a single circular chromosome made up of DNA, along with smaller circular pieces of DNA called plasmids. These bacterial genetic materials contain the information necessary for the growth and reproduction of the organism.

Bacterial RNA can be divided into three main categories: messenger RNA (mRNA), ribosomal RNA (rRNA), and transfer RNA (tRNA). mRNA carries genetic information copied from DNA, which is then translated into proteins by the rRNA and tRNA molecules. rRNA is a structural component of the ribosome, where protein synthesis occurs, while tRNA acts as an adapter that brings amino acids to the ribosome during protein synthesis.

Bacterial RNA plays a crucial role in various cellular processes, including gene expression, protein synthesis, and regulation of metabolic pathways. Understanding the structure and function of bacterial RNA is essential for developing new antibiotics and other therapeutic strategies to combat bacterial infections.

Rhizobium etli is a gram-negative, aerobic, motile, non-spore forming bacteria that belongs to the Rhizobiaceae family. It has the ability to fix atmospheric nitrogen in a symbiotic relationship with certain leguminous plants, particularly common bean (Phaseolus vulgaris). This bacterium infects the roots of these plants and forms nodules where it converts nitrogen gas into ammonia, a form that can be used by the plant for growth. The nitrogen-fixing ability of Rhizobium etli makes it an important bacteria in agriculture and environmental science.

I'm sorry for any confusion, but "Host Factor 1 Protein" is not a standard or widely recognized medical term or protein name in the context of human biology or medicine. It's possible that you may be referring to a specific protein involved in host-pathogen interactions in the field of microbiology, but without more context, it's difficult for me to provide an accurate and helpful definition.

In general, a "host factor" refers to any cellular component or process in the host organism (in this case, a human) that can affect the behavior or replication of a pathogen, such as a virus or bacterium. Host factors can include proteins, DNA sequences, RNA molecules, and other types of biomolecules.

If you have more information about the specific protein or context you're interested in, I'd be happy to try to help you further!

Nitrogen is not typically referred to as a medical term, but it is an element that is crucial to medicine and human life.

In a medical context, nitrogen is often mentioned in relation to gas analysis, respiratory therapy, or medical gases. Nitrogen (N) is a colorless, odorless, and nonreactive gas that makes up about 78% of the Earth's atmosphere. It is an essential element for various biological processes, such as the growth and maintenance of organisms, because it is a key component of amino acids, nucleic acids, and other organic compounds.

In some medical applications, nitrogen is used to displace oxygen in a mixture to create a controlled environment with reduced oxygen levels (hypoxic conditions) for therapeutic purposes, such as in certain types of hyperbaric chambers. Additionally, nitrogen gas is sometimes used in cryotherapy, where extremely low temperatures are applied to tissues to reduce pain, swelling, and inflammation.

However, it's important to note that breathing pure nitrogen can be dangerous, as it can lead to unconsciousness and even death due to lack of oxygen (asphyxiation) within minutes.

"Essential genes" refer to a category of genes that are vital for the survival or reproduction of an organism. They encode proteins that are necessary for fundamental biological processes, such as DNA replication, transcription, translation, and cell division. Mutations in essential genes often result in lethality or infertility, making them indispensable for the organism's existence. The identification and study of essential genes can provide valuable insights into the basic mechanisms of life and disease.

Erythritol is a type of sugar alcohol (a carbohydrate that is metabolized differently than other sugars) used as a sugar substitute in food and drinks. It has about 0.24 calories per gram and contains almost no carbohydrates or sugar, making it a popular choice for people with diabetes or those following low-carb diets. Erythritol is naturally found in some fruits and fermented foods, but most commercial erythritol is made from cornstarch. It has a sweet taste similar to sugar but contains fewer calories and does not raise blood sugar levels.

I'm sorry for any confusion, but "replicon" is not a term that has a widely accepted or specific medical definition. It is a term that is used in the field of molecular biology, where it refers to a segment of DNA that contains an origin of replication. The origin of replication is the site on the DNA molecule where the process of DNA replication is initiated. This concept is important in the fields of genetics and virology, but it is not a term that is commonly used in clinical medicine.

If you have any questions related to the medical field, I would be happy to try to help answer them for you!

Artificial gene fusion refers to the creation of a new gene by joining together parts or whole sequences from two or more different genes. This is achieved through genetic engineering techniques, where the DNA segments are cut and pasted using enzymes called restriction endonucleases and ligases. The resulting artificial gene may encode for a novel protein with unique functions that neither of the parental genes possess. This approach has been widely used in biomedical research to study gene function, create new diagnostic tools, and develop gene therapies.

Hydroxybutyrates are compounds that contain a hydroxyl group (-OH) and a butyric acid group. More specifically, in the context of clinical medicine and biochemistry, β-hydroxybutyrate (BHB) is often referred to as a "ketone body."

Ketone bodies are produced by the liver during periods of low carbohydrate availability, such as during fasting, starvation, or a high-fat, low-carbohydrate diet. BHB is one of three major ketone bodies, along with acetoacetate and acetone. These molecules serve as alternative energy sources for the brain and other tissues when glucose levels are low.

In some pathological states, such as diabetic ketoacidosis, the body produces excessive amounts of ketone bodies, leading to a life-threatening metabolic acidosis. Elevated levels of BHB can also be found in other conditions like alcoholism, severe illnesses, and high-fat diets.

It is important to note that while BHB is a hydroxybutyrate, not all hydroxybutyrates are ketone bodies. The term "hydroxybutyrates" can refer to any compound containing both a hydroxyl group (-OH) and a butyric acid group.

I'm happy to help! However, it seems like the term you're looking for is "Transfer RNA arginine," not "Arg." Transfer RNA (tRNA) is a type of RNA molecule that plays a crucial role in protein synthesis. Specifically, tRNAs are responsible for delivering amino acids to the ribosome, where they are joined together to form proteins.

Each tRNA molecule contains a specific anticodon sequence that can base-pair with a complementary codon sequence on messenger RNA (mRNA). When a tRNA molecule encounters an mRNA codon that matches its anticodon, it binds to the mRNA and delivers the amino acid associated with that tRNA.

In the case of tRNA arginine, this type of tRNA is responsible for delivering the amino acid arginine to the ribosome during protein synthesis. Arginine is a positively charged amino acid that plays important roles in various cellular processes, including protein structure and function, signal transduction, and gene regulation.

Therefore, Transfer RNA arginine refers to the specific tRNA molecule that delivers the amino acid arginine during protein synthesis.

'Glycyrrhiza uralensis', also known as Chinese licorice, is a plant species native to Asia. In a medical context, it often refers to the root of this plant, which contains various compounds with potential medicinal properties. It has been used in traditional medicine for centuries to treat various health conditions such as respiratory disorders, liver diseases, and skin inflammations.

The active component of Glycyrrhiza uralensis is glycyrrhizin, which has anti-inflammatory, antiviral, and expectorant properties. However, it should be noted that excessive consumption of glycyrrhizin can lead to serious side effects such as hypertension, hypokalemia, and edema. Therefore, it is important to use this herb under the guidance of a healthcare professional.

Gene deletion is a type of mutation where a segment of DNA, containing one or more genes, is permanently lost or removed from a chromosome. This can occur due to various genetic mechanisms such as homologous recombination, non-homologous end joining, or other types of genomic rearrangements.

The deletion of a gene can have varying effects on the organism, depending on the function of the deleted gene and its importance for normal physiological processes. If the deleted gene is essential for survival, the deletion may result in embryonic lethality or developmental abnormalities. However, if the gene is non-essential or has redundant functions, the deletion may not have any noticeable effects on the organism's phenotype.

Gene deletions can also be used as a tool in genetic research to study the function of specific genes and their role in various biological processes. For example, researchers may use gene deletion techniques to create genetically modified animal models to investigate the impact of gene deletion on disease progression or development.

"Oscillatoria" is not a medical term, but rather a taxonomic genus name in the field of biology and microbiology. It belongs to the family of cyanobacteria (blue-green algae) called "Pseudanabaenaceae." Oscillatoria species are filamentous bacteria that contain chlorophyll and can perform photosynthesis. They form long, straight or slightly curved trichomes (filaments) without heterocysts or akinetes. The cells in the trichome are typically separated by narrow gaps, giving them a beaded appearance.

These organisms are often found in various aquatic environments such as freshwater, brackish water, and marine habitats. Some species of Oscillatoria can produce toxins, known as cyanotoxins, which may pose health risks to humans and animals when they contaminate drinking water sources or recreational bodies of water.

In a medical context, exposure to harmful algal blooms containing Oscillatoria species might lead to symptoms such as skin irritation, allergic reactions, gastrointestinal issues, or respiratory problems in sensitive individuals. However, it is essential to note that these health effects are not exclusive to Oscillatoria and can be caused by various cyanobacterial genera.

Aminoethylphosphonic acid is a chemical compound with the formula (HO)â‚‚P(O)CHâ‚‚CHâ‚‚NHâ‚‚. It is an organophosphorus compound that contains both phosphonic and amino groups. This compound is a colorless solid that is soluble in water and has various applications in industry, including as a corrosion inhibitor and a scale inhibitor in water treatment systems. It may also have potential uses in medicine, such as in the treatment of kidney stones, although its use in this context is still being studied.

Dicarboxylic acids are organic compounds containing two carboxyl groups (-COOH) in their molecular structure. The general formula for dicarboxylic acids is HOOC-R-COOH, where R represents a hydrocarbon chain or a functional group.

The presence of two carboxyl groups makes dicarboxylic acids stronger acids than monocarboxylic acids (compounds containing only one -COOH group). This is because the second carboxyl group contributes to the acidity of the molecule, allowing it to donate two protons in solution.

Examples of dicarboxylic acids include oxalic acid (HOOC-COOH), malonic acid (CH2(COOH)2), succinic acid (HOOC-CH2-CH2-COOH), glutaric acid (HOOC-(CH2)3-COOH), and adipic acid (HOOC-(CH2)4-COOH). These acids have various industrial applications, such as in the production of polymers, dyes, and pharmaceuticals.

I'm sorry for any confusion, but "Polyesters" is not a medical term. It is a term used in materials science and textile industry to describe a type of synthetic fiber made from polymers characterized by the presence of ester groups in their main chain. If you have any questions related to medical terminology or concepts, I'd be happy to help with those instead!

Gene order, in the context of genetics and genomics, refers to the specific sequence or arrangement of genes along a chromosome. The order of genes on a chromosome is not random, but rather, it is highly conserved across species and is often used as a tool for studying evolutionary relationships between organisms.

The study of gene order has also provided valuable insights into genome organization, function, and regulation. For example, the clustering of genes that are involved in specific pathways or functions can provide information about how those pathways or functions have evolved over time. Similarly, the spatial arrangement of genes relative to each other can influence their expression levels and patterns, which can have important consequences for phenotypic traits.

Overall, gene order is an important aspect of genome biology that continues to be a focus of research in fields such as genomics, genetics, evolutionary biology, and bioinformatics.

Phosphoadenosine phosphosulfate (PAPS) is not exactly a medical term, but a biochemical term. However, it is often referred to in the context of medical and biological research.

PAPS is a crucial molecule in the metabolism of living organisms and serves as the primary donor of sulfate groups in the process of sulfonation, which is a type of enzymatic modification that adds a sulfate group to various substrates such as proteoglycans, hormones, neurotransmitters, and xenobiotics. This process plays an essential role in several biological processes, including detoxification, signal transduction, and cell-cell recognition.

Therefore, PAPS is a critical molecule for maintaining proper physiological functions in the body, and its dysregulation has been implicated in various diseases, such as cancer, inflammation, and neurodevelopmental disorders.

Acyl Carrier Protein (ACP) is a small, acidic protein that plays a crucial role in the fatty acid synthesis process. It functions as a cofactor by carrying acyl groups during the elongation cycles of fatty acid chains. The ACP molecule has a characteristic prosthetic group known as 4'-phosphopantetheine, to which the acyl groups get attached covalently. This protein is highly conserved across different species and is essential for the production of fatty acids in both prokaryotic and eukaryotic organisms.

Bacterial chromosomes are typically circular, double-stranded DNA molecules that contain the genetic material of bacteria. Unlike eukaryotic cells, which have their DNA housed within a nucleus, bacterial chromosomes are located in the cytoplasm of the cell, often associated with the bacterial nucleoid.

Bacterial chromosomes can vary in size and structure among different species, but they typically contain all of the genetic information necessary for the survival and reproduction of the organism. They may also contain plasmids, which are smaller circular DNA molecules that can carry additional genes and can be transferred between bacteria through a process called conjugation.

One important feature of bacterial chromosomes is their ability to replicate rapidly, allowing bacteria to divide quickly and reproduce in large numbers. The replication of the bacterial chromosome begins at a specific origin point and proceeds in opposite directions until the entire chromosome has been copied. This process is tightly regulated and coordinated with cell division to ensure that each daughter cell receives a complete copy of the genetic material.

Overall, the study of bacterial chromosomes is an important area of research in microbiology, as understanding their structure and function can provide insights into bacterial genetics, evolution, and pathogenesis.

Molecular cloning is a laboratory technique used to create multiple copies of a specific DNA sequence. This process involves several steps:

1. Isolation: The first step in molecular cloning is to isolate the DNA sequence of interest from the rest of the genomic DNA. This can be done using various methods such as PCR (polymerase chain reaction), restriction enzymes, or hybridization.
2. Vector construction: Once the DNA sequence of interest has been isolated, it must be inserted into a vector, which is a small circular DNA molecule that can replicate independently in a host cell. Common vectors used in molecular cloning include plasmids and phages.
3. Transformation: The constructed vector is then introduced into a host cell, usually a bacterial or yeast cell, through a process called transformation. This can be done using various methods such as electroporation or chemical transformation.
4. Selection: After transformation, the host cells are grown in selective media that allow only those cells containing the vector to grow. This ensures that the DNA sequence of interest has been successfully cloned into the vector.
5. Amplification: Once the host cells have been selected, they can be grown in large quantities to amplify the number of copies of the cloned DNA sequence.

Molecular cloning is a powerful tool in molecular biology and has numerous applications, including the production of recombinant proteins, gene therapy, functional analysis of genes, and genetic engineering.

Sinorhizobium meliloti is an agronomically relevant α-proteobacterium able to induce the formation of new specialized organs, ... Jones KM, Kobayashi H, Davies BW, Taga ME, Walker GC (2007). "How rhizobial symbionts invade plants: the Sinorhizobium-Medicago ... "Identification of differentially expressed small non-coding RNAs in the legume endosymbiont Sinorhizobium meliloti by ...
Sinorhizobium fredii NGR234). Find diseases associated with this biological target and compounds tested against it in bioassay ...
... from Sinorhizobium meliloti in complex with NADPH and oxalate ... from Sinorhizobium meliloti in complex with NADPH and oxalate. ... Sinorhizobium meliloti 1021. Mutation(s): 0 Gene Names: R00152, SMc02828. EC: 1.1.1.79. ... We compare two representatives of these subfamilies from Sinorhizobium meliloti (SmGhrA and SmGhrB), employing a combination of ...
The regulation of iron was studied in Sinorhizobium mehloti, which is a free-living Gram-negative bacteria found in soil and ... Viguier, Caroline (2005) Genetic regulation of iron responsive genes in sinorhizobium meliloti. PhD thesis, Dublin City ...
In this work, we evaluate how the co-inoculation of alfalfa with Sinorhizobium meliloti U143 and... ... In this work, we evaluate how the co-inoculation of alfalfa with Sinorhizobium meliloti U143 and JD2 increases plant yield ... The Pattern of Secreted Molecules During the Co-Inoculation of Alfalfa Plants With Sinorhizobium meliloti and Delftia sp. ...
The response of Medicago truncatula/Sinorhizobium medicae symbiotic plants supplied exclusively by symbiotic nitrogen fixation ... A localized osmotic stress activates systemic responses to N-limitation in Medicago truncatula-Sinorhizobium symbiotic plants. ... The response of Medicago truncatula/Sinorhizobium medicae symbiotic plants supplied exclusively by symbiotic nitrogen fixation ... A localized osmotic stress activates systemic responses to N-limitation in Medicago truncatula-Sinorhizobium symbiotic plants. ...
strain NGR234); Sinorhizobium fredii NGR234; Sinorhizobium sp. NGR234; Rhizobium NGR234; Word Map for Organism Sinorhizobium ...
... and we have previously defined a PcaQ DNA-binding site located upstream of the target pcaDCHGB operon in Sinorhizobium meliloti ... MacLean A. M., MacPherson G., Aneja P., Finan T. M. ( 2006). Characterization of the β-ketoadipate pathway in Sinorhizobium ... MacLean A. M. ( 2008). Study of saprophytic competence in Sinorhizobium meliloti McMaster University; Hamilton, ON, Canada:. [ ... The LysR-type PcaQ protein regulates expression of a protocatechuate-inducible ABC-type transport system in Sinorhizobium ...
HMMer finds a hit (regardless of coverage or other bits). Other blast hits with at least 50% coverage are "low confidence." Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to: ...
HMMer finds a hit (regardless of coverage or other bits). Other blast hits with at least 50% coverage are "low confidence." Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to: ...
name=FMN regulon. species= Sinorhizobium meliloti 1021. (optional)size=1. ...
Interpretive Summary: Sinorhizobium sp. strain MUS10 forms nodules on the roots and stems of Sesbania rostrata, a green manure ... Technical Abstract: Sinorhizobium sp. strain MUS10 forms nitrogen-fixing stem nodules on Sesbania rostrata, a tropical green- ... Sinorhizobium sp. strain MUS10 entered the host tissue through cracks created by the emerging adventitious root primordia and ... Title: AN ULTRASTRUCTURAL INVESTIGATION OF INFECTION THREADS IN SESBANIA ROSTRATA STEM NODULES INDUCED BY SINORHIZOBIUM SP. ...
Sinorhizobium meliloti. 5.5 - 9.5. Associated Proteins Protein name. Organism. Pyridoxal phosphate phosphatase YbhA. ...
Cubo, M.T.; Alías-Villegas, C.; Balsanelli, E.; Mesa, D.; de Souza, E.; Espuny, M.R. Diversity of Sinorhizobium (Ensifer) ...
The response of Medicago truncatula/Sinorhizobium medicae symbiotic plants supplied exclusively by symbiotic nitrogen fixation ... A localized osmotic stress activates systemic responses to N-limitation in Medicago truncatula-Sinorhizobium symbiotic plants. ... The response of Medicago truncatula/Sinorhizobium medicae symbiotic plants supplied exclusively by symbiotic nitrogen fixation ... A localized osmotic stress activates systemic responses to N-limitation in Medicago truncatula-Sinorhizobium symbiotic plants. ...
Sinorhizobium fredii HH103 Invades Lotus burttii by Crack Entry in a Nod Factor-and Surface Polysaccharide-Dependent Manner.. ... Sinorhizobium fredii HH103 RirA Is Required for Oxidative Stress Resistance and Efficient Symbiosis with Soybean.. Crespo-Rivas ... Sinorhizobium fredii HH103 bacteroids are not terminally differentiated and show altered O-antigen in nodules of the Inverted ... The Sinorhizobium (Ensifer) fredii HH103 Nodulation Outer Protein NopI Is a Determinant for Efficient Nodulation of Soybean and ...
A field study with genetically engineered alfalfa inoculated with recombinant Sinorhizobium meliloti: effects on the soil ... A field study with genetically engineered alfalfa inoculated with recombinant Sinorhizobium meliloti: effects on the soil ... A field study with genetically engineered alfalfa inoculated with recombinant Sinorhizobium meliloti: effects on the soil ...
B: Bradyrhizobium and S: Sinorhizobium. The clustering of isolates and their distribution throughout the total studied area is ...
Sinorhizobium sp. BR816 [gbbct]: 20 CDSs (6139 codons) fields: [triplet] [frequency: per thousand] ([number]) UUU 9.3( 57) UCU ...
Succinoglycan Production Contributes to Acidic pH Tolerance in Sinorhizobium meliloti Rm1021. Hawkins JP, Geddes BA, Oresnik IJ ...
Shipman, L.W., Li, D., Roessner, C.A., Scott, A.I., Sacchettini, J.C ...
This group is composed of mostly uncharacterized proteins including Sinorhizobium fredii CYPBJ-4 homolog ... This group is composed of mostly uncharacterized proteins including Sinorhizobium fredii CYPBJ-4 homolog. It belongs to the ...
Sinorhizobium meliloti establishes a nitrogen-fixing symbiosis with Medicago species. In the soilenvironment both partners need ... It is widely recognizable that the Medicago-Sinorhizobium symbiosis is highly sensitive to acid stress. To cope with low pH, S ...
2017). Negative regulation of ectoine uptake and catabolism in Sinorhizobium meliloti: characterization of the EhuR Gene. J. ...
Sinorhizobium. McMaster University. Peak area normalized. ST000259. AN000412. Metabolic contribution of pSymA and pSymB ... Sinorhizobium. McMaster University. Peak area normalized. ST000698. AN001083. Metabolomics analysis of Sirt5 knockdown (KD) ... Metabolic contribution of pSymA and pSymB megaplasmid/chromid for multipartite Sinorhizobium meliloti cultured in rich LBmc ... megaplasmid/chromid for multipartite Sinorhizobium meliloti cultured in rich LBmc medium. Bacterial cells. ...
"Computational Analysis of Sinorhizobium meliloti ExoR Protein." 50th Annual Meeting of the American Society for Cell Biology. ... Wiech, E., Cheng, H., and Singh, S. Computational analyses of the ExoS protein, a component of Sinorhizobium meliloti ExoR-ExoS ... "Molecular modeling and computational analyses suggests that the Sinorhizobium meliloti periplasmic regulator protein ExoR ...
Role of trehalose transport and utilization in Sinorhizobium meliloti--alfalfa interactions.. Journal. Mol Plant Microbe ...
Evolutionary relationship of Sinorhizobium meliloti and Sinorhizobium medicae strains isolated from Caucasian region. FEMS ... Genetic relationship of Sinorhizobium meliloti and Sinorhizobium medicae strains isolated from Caucasian region. FEMS ... L.Ferri; A.Gori; E.G.Biondi; A.Mengoni; M.Bazzicalupo (2010). Plasmid electroporation of Sinorhizobium strains: The role of the ... D.Trabelsi D; A.Mengoni; M.E.Aouani; M.Bazzicalupo; R.Mhamdi (2010). Genetic diversity and salt tolerance of Sinorhizobium ...
Flavones and flavonols play distinct critical roles during nodulation of Medicago truncatula by Sinorhizobium meliloti. Plant J ...
  • Most of these strains have been clustered into the species Sinorhizobium fredii. (uni-bielefeld.de)
  • This group is composed of mostly uncharacterized proteins including Sinorhizobium fredii CYPBJ-4 homolog. (nih.gov)
  • The Soybean Rfg1 Gene Restricts Nodulation by Sinorhizobium fredii USDA193. (proteinlounge.com)
  • Modificaci n gen tica de leguminosas y rhizobium para su uso en biorremediaci n. (us.es)
  • Rhizobium, Bradyrhizobium, Mesorhizobium, Sinorhizobium and Azorhizobium-known as Rhizobia-are Gram-negative, nitrogen-fixing bacteria of agronomic importance because they perform nitrogen-fixing symbiosis with leguminous plants. (proteinlounge.com)
  • Glycine max (soybean) plants can be nodulated by fast-growing rhizobial strains of the genus Sinorhizobium as well as by slow-growing strains clustered in the genus Bradyrhizobium. (uni-bielefeld.de)
  • Sinorhizobium meliloti is an agronomically relevant α-proteobacterium able to induce the formation of new specialized organs, the so-called nodules, in the roots of its cognate legume hosts (i.e. some Medicago species). (wikipedia.org)
  • The regulation of iron was studied in Sinorhizobium mehloti, which is a free-living Gram-negative bacteria found in soil and also as an endosymbiont o f Medicago sativa (alfalfa). (dcu.ie)
  • Sinorhizobium meliloti establishes a nitrogen-fixing symbiosis with Medicago species. (conicet.gov.ar)
  • It is widely recognizable that the Medicago-Sinorhizobium symbiosis is highly sensitive to acid stress. (conicet.gov.ar)
  • Here we use whole-genome resequencing of a hierarchically-structured sample of 191 strains of Sinorhizobium meliloti collected from 21 locations in southern Europe to characterize the population structures of this bacterial symbiont and its host plant Medicago truncatula . (datadryad.org)
  • The lipopolysaccharide of Sinorhizobium meliloti suppresses defense-associated gene expression in cell cultures of the host plant Medicago truncatula. (mpg.de)
  • The interaction of M. truncatula with the bacterial symbiont Sinorhizobium meliloti is characterized by the formation of root nodules that fix atmospheric nitrogen. (jic.ac.uk)
  • Creation and Characterization of a Genomically Hybrid Strain in the Nitrogen-Fixing Symbiotic Bacterium Sinorhizobium meliloti. (unifi.it)
  • This enhanced conjugation was further replicated with a different bacterial species- Sinorhizobium meliloti, as the pSC5-carrying donor, suggesting that this mechanism can be used with different bacteria. (eurekalert.org)
  • Role and regulation of ACC deaminase gene in Sinorhizobium meliloti: is it a symbiotic, rhizospheric or endophytic gene? (unifi.it)
  • Role of trehalose transport and utilization in Sinorhizobium meliloti--alfalfa interactions. (genome.jp)
  • The biochemistry of cellobiose utilization by Sinorhizobium meliloti (cellobiose is a glucose-containing disaccharide)," Myhanh Chu working with Dr. Wacks. (redlands.edu)
  • The Pattern of Secreted Molecules During the Co-Inoculation of Alfalfa Plants With Sinorhizobium meliloti and Delftia sp. (apsnet.org)
  • In this work, we evaluate how the co-inoculation of alfalfa with Sinorhizobium meliloti U143 and JD2 increases plant yield under greenhouse conditions and we analyze the pattern of secreted bioactive compounds which may be involved in the microbe-plant communication. (apsnet.org)
  • Molecular modeling and computational analyses suggests that the Sinorhizobium meliloti periplasmic regulator protein ExoR adopts a superhelical fold and is controlled by a unique mechanism of proteolysis. (cuny.edu)
  • The genetic characterization of a 5.5-kb chromosomal region of Sinorhizobium meliloti 2011 that contains lpsB, a gene required for the normal development of symbiosis with Medicago spp. (uni-bielefeld.de)
  • This research has been undertaken to evaluate the effect of Cd tolerance of native rhizobial strains on diminution of the Cd detrimental effects on Sinorhizobium meliloti -alfalfa symbiosis. (ac.ir)
  • 12. Contributions of Sinorhizobium meliloti Transcriptional Regulator DksA to Bacterial Growth and Efficient Symbiosis with Medicago sativa. (nih.gov)
  • 17. Heterologous Complementation Reveals a Specialized Activity for BacA in the Medicago-Sinorhizobium meliloti Symbiosis. (nih.gov)
  • Phosphorylation of the transcription factor RmFixJ is the key step4 in the hypoxic induction of Sinorhizobium meliloti nitrogen fixation genes. (elsevierpure.com)
  • QS system of Sinorhizobium meliloti composed of at least three genes of sinI (autoinducer synthase), sinR and expR (autoinducer activated receptor) which involvedin its free living and symbiotic functions. (ac.ir)
  • 18. Novel Genes and Regulators That Influence Production of Cell Surface Exopolysaccharides in Sinorhizobium meliloti. (nih.gov)
  • The BacA protein is essential for the long-term survival of Sinorhizobium meliloti and Brucella abortus within acidic compartments in plant and animal cells, respectively. (ecu.edu)
  • For amylase activity, three strains Sinorhizobium sp. (journalcra.com)
  • Among the eight strains, Sinorhizobium sp.MRR 101, S.kostiense MRR 104, and E.xinjiangense MRR 110 were proved to be better as plant growth promoting rhizobateria. (journalcra.com)
  • Kühn, A., Yu, S. and Giffhorn, F. Catabolism of 1,5-anhydro- D -fructose in Sinorhizobium morelense S-30.7.5: discovery, characterization, and overexpression of a new 1,5-anhydro- D -fructose reductase and its application in sugar analysis and rare sugar synthesis. (enzyme-database.org)
  • 10. Transcriptome Response to Heavy Metals in Sinorhizobium meliloti CCNWSX0020 Reveals New Metal Resistance Determinants That Also Promote Bioremediation by Medicago lupulina in Metal-Contaminated Soil. (nih.gov)
  • The Sinorhizobium PubMLST sequence definition database contains allele and profile data representing the known diversity of S. meliloti and S. medicae . (pubmlst.org)
  • Functional diversity of five homologous Cu+-ATPases present in Sinorhizobium meliloti. (umassmed.edu)
  • In Sinorhizobium morelense , the product of the reaction, 1,5-anhydro- D -mannitol, can be further metabolized to D -mannose [1]. (enzyme-database.org)
  • Dambe, T.R., Kühn, A.M., Brossette, T., Giffhorn, F. and Scheidig, A.J. Crystal structure of NADP(H)-dependent 1,5-anhydro- D -fructose reductase from Sinorhizobium morelense at 2.2 Ã… resolution: construction of a NADH-accepting mutant and its application in rare sugar synthesis. (enzyme-database.org)