A sequence-ready BAC clone contig of a 2.2-Mb segment of human chromosome 1q24. (1/680)

Human chromosomal region 1q24 encodes two cloned disease genes and lies within large genetic inclusion intervals for several disease genes that have yet to be identified. We have constructed a single bacterial artificial chromosome (BAC) clone contig that spans over 2 Mb of 1q24 and consists of 78 clones connected by 100 STSs. The average density of mapped STSs is one of the highest described for a multimegabase region of the human genome. The contig was efficiently constructed by generating STSs from clone ends, followed by library walking. Distance information was added by determining the insert sizes of all clones, and expressed sequence tags (ESTs) and genes were incorporated to create a partial transcript map of the region, providing candidate genes for local disease loci. The gene order and content of the region provide insight into ancient duplication events that have occurred on proximal 1q. The stage is now set for further elucidation of this interesting region through large-scale sequencing.  (+info)

Der(22) syndrome and velo-cardio-facial syndrome/DiGeorge syndrome share a 1.5-Mb region of overlap on chromosome 22q11. (2/680)

Derivative 22 (der[22]) syndrome is a rare disorder associated with multiple congenital anomalies, including profound mental retardation, preauricular skin tags or pits, and conotruncal heart defects. It can occur in offspring of carriers of the constitutional t(11;22)(q23;q11) translocation, owing to a 3:1 meiotic malsegregation event resulting in partial trisomy of chromosomes 11 and 22. The trisomic region on chromosome 22 overlaps the region hemizygously deleted in another congenital anomaly disorder, velo-cardio-facial syndrome/DiGeorge syndrome (VCFS/DGS). Most patients with VCFS/DGS have a similar 3-Mb deletion, whereas some have a nested distal deletion endpoint resulting in a 1.5-Mb deletion, and a few rare patients have unique deletions. To define the interval on 22q11 containing the t(11;22) breakpoint, haplotype analysis and FISH mapping were performed for five patients with der(22) syndrome. Analysis of all the patients was consistent with 3:1 meiotic malsegregation in the t(11;22) carrier parent. FISH-mapping studies showed that the t(11;22) breakpoint occurred in the same interval as the 1.5-Mb distal deletion breakpoint for VCFS. The deletion breakpoint of one VCFS patient with an unbalanced t(18;22) translocation also occurred in the same region. Hamster-human somatic hybrid cell lines from a patient with der(22) syndrome and a patient with VCFS showed that the breakpoints occurred in an interval containing low-copy repeats, distal to RANBP1 and proximal to ZNF74. The presence of low-copy repetitive sequences may confer susceptibility to chromosome rearrangements. A 1.5-Mb region of overlap on 22q11 in both syndromes suggests the presence of dosage-dependent genes in this interval.  (+info)

GABA- and glutamate-activated channels in green fluorescent protein-tagged gonadotropin-releasing hormone neurons in transgenic mice. (3/680)

Mice were generated expressing green fluorescent protein (GFP) under the control of the gonadotropin-releasing hormone (GnRH) promoter. Green fluorescence was observed in, and restricted to, GnRH-immunopositive neuronal somata in the olfactory bulb, ganglion terminale, septal nuclei, diagonal band of Broca (DBB), preoptic area (POA), and caudal hypothalamus, as well as GnRH neuronal dendrites and axons, including axon terminals in the median eminence and organum vasculosum of the lamina terminalis (OVLT). Whole-cell recordings from GFP-expressing GnRH neurons in the OVLT-POA-DBB region revealed a firing pattern among GFP-expressing GnRH neurons distinct from that of nonfluorescent neurons. Nucleated patches of GFP-expressing GnRH neurons exhibited pronounced responses to fast application of GABA and smaller responses to L-glutamate and AMPA. One-fifth of the nucleated patches responded to NMDA. The GABA-A, AMPA, and NMDA receptor channels on GnRH neurons mediating these responses may play a role in the modulation of GnRH secretory oscillations.  (+info)

Analysis of sequence-tagged-connector strategies for DNA sequencing. (4/680)

The BAC-end sequencing, or sequence-tagged-connector (STC), approach to genome sequencing involves sequencing the ends of BAC inserts to scatter sequence tags (STCs) randomly across the genome. Once any BAC or other large segment of DNA is sequenced to completion by conventional shotgun approaches, these STC tags can be used to identify a minimum tiling path of BAC clones overlapping the nucleation sequence for sequence extension. Here, we explore the properties of STC-sequencing strategies within a mathematical model of a random target with homologous repeats and imperfect sequencing technology to understand the consequences of varying various parameters on the incidence of problem clones and the cost of the sequencing project. Problem clones are defined as clones for which either (A) there is no identifiable overlapping STC to extend the sequence in a particular direction or (B) the identified STC with minimum overlap comes from a nonoverlapping clone, either owing to random false matches or repeat-family homology. Based on the minimum overlap, we estimate the number of clones to be entirely sequenced and, then, using cost estimates, identify the decision rule (the degree of sequence similarity required before a match is declared between an STC and a clone) to minimize overall sequencing cost. A method to optimize the overlap decision rule is highly desirable, because both the total cost and the number of problem clones are shown to be highly sensitive to this choice. For a target of 3 Gb containing approximately 800 Mb of repeats with 85%-90% identity, we expect <10 problem clones with 15 times coverage by 150-kb clones. We derive the optimal redundancy and insert sizes of clone libraries for sequencing genomes of various sizes, from microbial to human. We estimate that establishing the resource of STCs as a means of identifying minimally overlapping clones represents only 1%-3% of the total cost of sequencing the human genome, and, up to a point of diminishing returns, a larger STC resource is associated with a smaller total sequencing cost.  (+info)

An integrated map of chromosome 18 CAG trinucleotide repeat loci. (5/680)

Expansions of trinucleotide CAG repeats have been demonstrated in at least eight neurodegenerative disorders, and suggested to occur in several others, including bipolar disorder and schizophrenia. Chromosome 18 loci have been implicated in bipolar disorder pedigrees by linkage analysis. To address this putative link between chromosome 18 CAG trinucleotide repeats and neuropsychiatric illness, we have screened a chromosome 18 cosmid library (LL18NCO2" AD") and identified 14 novel candidate loci. Characterisation of these loci involved repeat flank sequencing, estimation of polymorphism frequency and mapping using FISH as well as radiation hybrid panels. These mapped trinucleotide loci will be useful in the investigation of chromosome 18 in neurodegenerative or psychiatric conditions, and will serve to integrate physical and radiation hybrid maps of chromosome 18.  (+info)

A contiguous 3-Mb sequence-ready map in the S3-MX region on 21q22.2 based on high- throughput nonisotopic library screenings. (6/680)

Progress in complete genomic sequencing of human chromosome 21 relies on the construction of high-quality bacterial clone maps spanning large chromosomal regions. To achieve this goal, we have applied a strategy based on nonradioactive hybridizations to contig building. A contiguous sequence-ready map was constructed in the Down syndrome congenital heart disease (DS-CHD) region in 21q22.2, as a framework for large-scale genomic sequencing and positional candidate gene approach. Contig assembly was performed essentially by high throughput nonisotopic screenings of genomic libraries, prior to clone validation by (1) restriction digest fingerprinting, (2) STS analysis, (3) Southern hybridizations, and (4) FISH analysis. The contig contains a total of 50 STSs, of which 13 were newly isolated. A minimum tiling path (MTP) was subsequently defined that consists of 20 PACs, 2 BACs, and 5 cosmids covering 3 Mb between D21S3 and MX1. Gene distribution in the region includes 9 known genes (c21-LRP, WRB, SH3BGR, HMG14, PCP4, DSCAM, MX2, MX1, and TMPRSS2) and 14 new additional gene signatures consisting of cDNA selection products and ESTs. Forthcoming genomic sequence information will unravel the structural organization of potential candidate genes involved in specific features of Down syndrome pathogenesis.  (+info)

Angiopoietin-3, a novel member of the angiopoietin family. (7/680)

A cDNA clone encoding angiopoietin-3 protein (Ang3), a novel member of the angiopoietin family, was identified. Ang3 cDNA was cloned from a human aorta cDNA library. Ang3 is a 503 amino acid protein having 45.1% and 44.7% identity with human angiopoietin-1 and human angiopoietin-2, respectively. Ang3 mRNA is expressed in lung and cultured human umbilical vein endothelial cells (HUVECs). Ang3 mRNA expression in HUVECs was slightly decreased by vascular endothelial cell growth factor treatment, suggesting that the regulation of Ang3 mRNA expression is different from that of Ang2.  (+info)

Revealing hidden interval graph structure in STS-content data. (8/680)

MOTIVATION: STS-content data for genomic mapping contain numerous errors and anomalies resulting in cross-links among distant regions of the genome. Identification of contigs within the data is an important and difficult problem. RESULTS: This paper introduces a graph algorithm which creates a simplified view of STS-content data. The shape of the resulting structure graph provides a quality check - coherent data produce a straight line, while anomalous data produce branches and loops. In the latter case, it is sometimes possible to disentangle the various paths into subsets of the data covering contiguous regions of the genome, i.e. contigs. These straight subgraphs can then be analyzed in standard ways to construct a physical map. A theoretical basis for the method is presented along with examples of its application to current STS data from human genome centers. AVAILABILITY: Freely available on request.  (+info)

"Sequence-Tagged Sites (STS)". Sequence-Tagged Sites (STS) description on NCBI Simple sequence length polymorphism v t e ( ... A sequence-tagged site (or STS) is a short (200 to 500 base pair) DNA sequence that has a single occurrence in the genome and ... They are used in shotgun sequencing, specifically to aid sequence assembly. STSs are very helpful for detecting microdeletions ... For this reason they are useful for constructing genetic and physical maps from sequence data reported from many different ...
Tn5 mediated cleavage produces a library of target DNA sites bound to a protein of interest in situ. Sequencing of prepared DNA ... Sensitivity of sequencing depends on the depth of the sequencing run (i.e. the number of mapped sequence tags), the size of the ... it suffers from a number of practical and economical limitations that CUT&RUN and CUT&Tag sequencing do not. CUT&Tag sequencing ... CUT&RUN is not suitable for single-cell platforms so CUT&Tag is advantageous for these. CUT&Tag-sequencing can be used to ...
Some tags are also inserted at sites within the protein of interest; they are known as internal tags. Affinity tags are ... such as FLAG-tag or polyglutamate tag. Epitope tags are short peptide sequences which are chosen because high-affinity ... Epitope tags include ALFA-tag, V5-tag, Myc-tag, HA-tag, Spot-tag, T7-tag and NE-tag. These tags are particularly useful for ... Protein tags are peptide sequences genetically grafted onto a recombinant protein. Tags are attached to proteins for various ...
Miwa T, Sudo K, Nakamura Y, Imai T (July 1993). "Fifty sequenced-tagged sites on human chromosome 11". Genomics. 17 (1): 211-4 ... Besides the interactions that position eS25 at its location in the E site region of the 40S ribosomal subunit, eS25 has been ... Li ML, Latoud C, Center MS (November 1991). "Cloning and sequencing a cDNA encoding human ribosomal protein S25". Gene. 107 (2 ... July 1996). "Characterization of the human small-ribosomal-subunit proteins by N-terminal and internal sequencing, and mass ...
demonstrate the application of the aldehyde tag as a chemical conjugation site for glycans. The aldehyde tag sequence was ... The 6-mer tag represents the small core consensus sequence and the 13-mer tag the longer full motif. The experiments on the ... The formylglycine tag or aldehyde tag is a convenient 6- or 13-amino acids long tag fused to a protein of interest. ... The size of the sequence is analogous to the commonly used 6x His-Tag and has the advantage that it can also be genetically ...
Dec 1993). "A sequence-tagged site map of human chromosome 11". Genomics. 17 (3): 699-725. doi:10.1006/geno.1993.1392. PMID ... 2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40-5. doi:10.1038/ ... Shark KB, Lee NM (1995). "Cloning, sequencing and localization to chromosome 11 of a cDNA encoding a human opioid-binding cell ... 2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci ...
September 1993). "A sequence-tagged site map of human chromosome 11". Genomics. 17 (3): 699-725. doi:10.1006/geno.1993.1392. ... The uridine moiety of tunicamycin occupies the identical binding sites of UDP-GlcNAc. The lipid tail moiety of tunicamycin ... DNA Sequence. 13 (5): 245-250. doi:10.1080/1042517021000017126. PMID 12592703. S2CID 25176842. Newell JW, Seo NS, Enns GM, ...
... or upon further sequencing (since tag sites are unique enough to serve as primer annealing sites). Paired-end tags (PET) exist ... linker sequence, a short 5' sequence tag, a short 3' sequence tag, and a short 3' linker sequence. It was shown conceptually ... On average, 20-30 tags could be sequenced with the Sanger method, which has a longer read length. Since the tag sequences are ... The cloning sites are flanked with adaptor sequences that contain restriction sites for endonucleases (discussed below). ...
... by a suitable amino acid sequence that facilitates removal of the polyhistidine-tag using endopeptidases. This extra sequence ... "His-tag: The timeless standard for protein purification". Cube Biotech Knowledge Site. Archived from the original on 15 ... The HN tag binds to the immobilized metal ion more efficiently than the His tag. The HAT tag is a peptide tag ( ... It is also known as a hexa histidine-tag, 6xHis-tag, or His6 tag. The tag was invented by Roche, although the use of histidines ...
1993). "The development of sequence-tagged sites for human chromosome 4". Hum. Mol. Genet. 2 (8): 1271-88. doi:10.1093/hmg/2.8. ... Isolation of a full length cDNA and comparative sequence analyses of orthologous and paralogous proteins". J. Biol. Chem. 260 ( ...
Premier software supports motif analysis and ORF search along with finding restriction cut sites on the nucleotide sequence. ... The software also designs tagged and nested primers for multiplex PCR reactions. The Primer ... Primer Premier Homepage (All articles with bare URLs for citations, Articles with bare URLs for citations from March 2022, ... The software has a built-in nucleotide-protein and protein-nucleotide sequence translator which can be used for designing ...
The complementary sequence is then 3'-CTA,TAG-5'. The ends are blunt and can be ligated into a blunt cloning site easily but ... The structure of this enzyme, and several mutants, in complex with the DNA sequence which it cuts has been solved by X-ray ... Initially the enzyme binds weakly to a non-specific site on the DNA. It randomly walks along the molecule until the specific ... recognition site is found. EcoRV has a high specificity for its target DNA sequence. Binding of the enzyme induces a ...
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). Additionally, FLAG-tags may ... with the final tag encoding an enterokinase cleavage site). FLAG-tag can be fused to the C-terminus or the N-terminus of a ... The FLAG-tag can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or myc-tag ... The FLAG-tag's sequence was optimized for compatibility with proteins it is attached to, in that FLAG-tag is more hydrophilic ...
1997). "Generation and analysis of 280,000 human expressed sequence tags". Genome Res. 6 (9): 807-28. doi:10.1101/gr.6.9.807. ... Hartley JL, Temple GF, Brasch MA (2001). "DNA Cloning Using In Vitro Site-Specific Recombination". Genome Res. 10 (11): 1788-95 ... 2004). "Sequence Comparison of Human and Mouse Genes Reveals a Homologous Block Structure in the Promoter Regions". Genome Res ... 2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40-5. doi:10.1038/ ...
"Gene-based sequence-tagged-sites (STSs) as the basis for a human gene map". Nat Genet. 10 (4): 415-23. doi:10.1038/ng0895-415. ... 2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci ... 2006). "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks". Cell. 127 (3): 635-48. doi:10.1016/ ...
"Gene-based sequence-tagged-sites (STSs) as the basis for a human gene map". Nat. Genet. 10 (4): 415-23. doi:10.1038/ng0895-415 ...
August 1995). "Gene-based sequence-tagged-sites (STSs) as the basis for a human gene map". Nature Genetics. 10 (4): 415-423. ... Tellier M, Chalmers R (January 2019). "Human SETMAR is a DNA sequence-specific histone-methylase with a broad effect on the ... 2021-01-01). "Genome-wide mapping of binding sites of the transposase-derived SETMAR protein in the human genome". ... April 1997). "Large-scale concatenation cDNA sequencing". Genome Research. 7 (4): 353-358. doi:10.1101/gr.7.4.353. PMC 139146. ...
Rather than individual code points, they are represented by tag sequences. Speaking to Crikey in 2022, Burge spoke against ... The Sydney Morning Herald reported the site had 23 million page views per month in 2017. Burge was Chief Emoji Officer at ... In addition, Burge has spoken at conferences such as The Next Web in Amsterdam, Smart Future in Riga, Design Matters in ... Mix (12 August 2020). "Why skin tone modifiers don't work for 🤝, explained by an emoji historian". The Next Web. Archived from ...
The tagged DNA fragments are then purified, PCR-amplified, and sequenced using next-generation sequencing. Sequencing reads can ... adapted to map DNA methylation sites, or combined with sequencing techniques. The utility of high-resolution enhancer mapping ... In a process called "tagmentation", Tn5 transposase cleaves and tags double-stranded DNA with sequencing adaptors. ... The most common use is nucleosome mapping experiments, but it can be applied to mapping transcription factor binding sites, ...
If a new phenotype is observed, the genome is sequenced and searched for tagged transposons. If the site of transposon ... Naturally occurring P elements contain: coding sequence for the enzyme transposase; recognition sequences for transposase ... This sequence was then added inserted into a plasmid and introduced into the cells of the mice. The transposase from Sleeping ... In STM, specially tagged transposons are inserted into an organism, such as a bacterium, and randomly integrated into the host ...
This web based service builds on the Tagenerator tool, but is very fast because all tags are pre-generated and stored in a ... Techniques such as RS-PCR and (EXACT) RT-PCR are based on the integration of such tags (unique sequences not present in genomic ... Lopes Pinto, Fernando; Håkan Svensson; Peter Lindblad (2007-10-25). "Webtag: a new web tool providing tags/anchors for RT-PCR ... The possibility of using tags whose sequences are not found in the genome further improves reverse specific polymerase chain ...
The most efficient way to find RAD tags is by high-throughput DNA sequencing, called RAD tag sequencing, RAD sequencing, RAD- ... The use of the flanking DNA sequences around each restriction site is an important aspect of RAD tags. The density of RAD tags ... disrupt restriction sites and lead to the absence of RAD tags or substantial DNA sequence polymorphisms that disrupt RAD tag ... The use of high-throughput sequencing to analyze RAD tags can be classified as reduced-representation sequencing, which ...
de Temmerman P, Visvikis S, Boerwinkle E, Siest G (1990). "Study of the sequence tagged site (STS) in the beginning of human ... a sequence polymorphism has also been identified in the 3'UTR of the third exon. Intra-species comparative gene sequence ... 1992). "Nonsynonymous polymorphic sites in the apolipoprotein (apo) A-IV gene are associated with changes in the concentration ... 1986). "The nucleotide and derived amino acid sequence of human apolipoprotein A-IV mRNA and the close linkage of its gene to ...
Also, due to the nature of the reliance on sequence tagged sites (STS) as a reference point when selecting appropriate clones, ... "Mapping human chromosomes by walking with sequence-tagged sites from end fragments of yeast artificial chromosome inserts". ... Ligation of artificial telomeric sequences to convert circular plasmid into a linear piece of DNA Insertion of DNA sequence to ... Ligation of necessary centromeric sequences for mitotic stability Ligation of Autonomously Replicating Sequences (ARS) ...
A sequence-tagged site (STS) is a short sequence of DNA (about 100 - 500 base pairs in length) that is seen to appear multiple ... as they differ from other sequences). Sequenced tagged sites can be mapped within our genome and require a group of overlapping ... These sites are easily recognizable, usually appearing at least once in the DNA being analyzed. These sites usually contain ... Now a good choice of clones can be made to efficiently sequence the clones to determine the DNA sequence of the organism under ...
... a small peptide of a known sequence was synthesized and tagged with a fluorescent molecule. This traceable peptide of sequence ... that would displace a fluorescein-labeled peptide derived from the eIF4G sequence that binds to the eIF4E form at the same site ... The sequence of 4EGI-1 was such that it displaced the labeled peptide, thus demonstrating its affinity for the complex binding ... eIF4E BPs (4E-BPs), as small polypeptides, consist of the same amino acid sequence as the portion of eIF4G that interacts with ...
These tags are then amplified by PCR with primers containing a mismatch changing the Bpm1 site to a Xho1 site. The amplicons ... CAGE has shown tags with addition of a guanine on the first position and oligo-capping may lead to sequence bias due to the use ... end of the messenger RNA require the presence of a trans-spliced leader sequence. Spliced leader sequences are short sequences ... These tagged cDNAs are then cleaved 14 bp downstream from the recognition site using BpmI restriction endonuclease and blunt ...
... with adaptor primers and AE and TE recognition sites attached) are ligated, sandwiching the two tag sequences together, and ... The sequencing length of the tag can be freely chosen. Because of this, the tags can be assembled into contigs and the ... who sequenced 178 clones from a rabbit muscle cDNA library. In 1991 Adams and co-workers coined the term expressed sequence tag ... The output of SAGE is a list of short sequence tags and the number of times it is observed. Using sequence databases a ...
Cut: Cutting site and DNA products of the cut. The recognition sequence and the cutting site usually match, but sometimes the ... C decreases TAG 3'". Nucleic Acids Res. 19 (5): 1152. doi:10.1093/nar/19.5.1152. PMC 333798. PMID 2020550. Azeddoug H, Reysset ... Recognition sequence: Sequence of DNA recognized by the enzyme and to which it specifically binds. ... A neoschizomer is a special type of isoschizomer that recognizes the same sequence as another, but cuts in a different manner. ...
... may optionally contain a probe-specific tag sequence that uniquely identifies the given probe as well as a tag-release site, ... The tags from each probe are released by cleaving the tag release site with restriction enzymes. These tags are then hybridized ... The tag sequences from the four nucleotide-specific reactions are then hybridized to either four genotyping arrays or two, dual ... Probe release In some versions of the protocol, the probe-release site (commonly a restriction site) is cleaved by restriction ...
"Sequence-Tagged Sites (STS)". Sequence-Tagged Sites (STS) description on NCBI Simple sequence length polymorphism v t e ( ... A sequence-tagged site (or STS) is a short (200 to 500 base pair) DNA sequence that has a single occurrence in the genome and ... They are used in shotgun sequencing, specifically to aid sequence assembly. STSs are very helpful for detecting microdeletions ... For this reason they are useful for constructing genetic and physical maps from sequence data reported from many different ...
... On-line free medical diagnosis assistant. Ranked list of possible diseases from either several symptoms ... Sequence tagged sites. Short tracts of dna sequence that are used as landmarks in genome mapping. In most instances, 200 to 500 ... base pairs of sequence define a Sequence Tagged Site (STS) that is operationally unique in the human genome (i.e., can be ... Home. Usage examples. Diseases. Frequent searches. Medicinal plants. Health topics. Medical dictionary. Health sites. Questions ...
Possible roles of endogenous plant viral sequences and transgenes containing viral sequences in both virus resistance and virus ... No HTML tags allowed. - Web page URLs will display as text only. - Lines and paragraphs break automatically. - Attachments, ... Possible roles of endogenous plant viral sequences and transgenes containing viral sequences in both virus resistance and virus ... Possible roles of endogenous plant viral sequences and transgenes containing viral sequences in both virus resistance and virus ...
Homlisti - Real Estate WordPress Theme Demo site access Homlisti is the best Real Estate WordPress theme with a crea... ... Fixed: Full site Heading sequence. Fixed: Agent listing count bug. Fixed: Some CSS issues. Updated: HomListi Core and ... Tags. agency, agent, custom fields, elementor, listing, maps, modern & clean, property, real estate, real estate agency, real ... These homepage demos are fully equipped with pages and elements that a real estate listing website needs. Since Homlisti has ...
The identity of the clones was checked by PCR using published sequence tagged sites and agrees with the contig published by ... Checking the identity of the clones by PCR of sequence tagged sites. Published STS primers (Xq3649, DXS7067, GDB:179459, ... Results showed a 618 bp sequence with 99% identity within each of the clones. In our experience this small section of sequence ... In our BAC/PAC sequence analysis, we identified a small 618 bp inverted repeat sequence within the genomic clones at either end ...
... potential gene initiation sites). It also illustrates the power of the STC strategy for creating minimum tiling paths of BAC ... The sequence-tagged connector (STC) strategy proposes to generate sequence tags densely scattered (every 3.3 kilobases) across ... Sequence-tagged connectors: a sequence approach to mapping and scanning the human genome. Mahairas GG, Wallace JC, Smith K, ... a minimum tiling path of clones can be sequenced at a rate that is primarily limited by the sequencing throughput of individual ...
Expression cDNA Clone or AA Sequence. Protein Sequence (showhide) >RC204803 protein sequence. Red=Cloning site Green=Tags(s). ... Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation ... Purified recombinant protein of human secreted phosphoprotein 1 (SPP1), transcript variant 3, with C-terminal MYC/DDK tag, ... SPP1 (Myc-DDK-tagged)-Human secreted phosphoprotein 1 (SPP1), transcript variant 1 ...
Internet Protocol (or IP) address, protocol, and sequence information. *Cookies. *Beacons. *Pixel tags ... Website Hosting: AA hosts its Website internally. (Certain event-related websites may be and Mobile Apps are hosted by third- ... The Website, Mobile App, and Services are not directed to children under the age of 16. You may not use the Website, Mobile App ... Third-party websites and mobile applications (e.g., websites that share information with us or advertising partners regarding ...
... fused to the N-terminus of the SNAP-tag use the available restriction sites ClaI ... To subclone the gene of interest into pSNAP-tag(m) ... The addition of a Kozak sequence (e.g. GCCRCCATG, where the ... Cloning of SNAP-tag Fusions in pSNAP-tag(m) (N9172). Protocol. * Cloning by PCR To subclone the gene of interest into pSNAP-tag ... To subclone the gene of interest into pSNAP-tag(m) fused to the C-Terminus of the SNAP-tag use the available restriction sites ...
For parallel expression of His-tagged proteins in E. coli, mammalian cells, and baculovirus-infected insect cells using a ... 2. Synthetic ribosomal binding site RBSII. For efficient translation. 3. His-tag coding sequence. Either 5 or 3 to the ... Home. Products. Discovery & Translational Research. Protein Purification. Tagged Protein Expression, Purification, Detection. ... "Protein minipreps of 6x His-tagged proteins from E. coli under native conditions" and Protocol 19. "6xHis-tagged protein ...
Remote Terminal Escape Sequence Injection. CVE-9158 . remote exploit for Linux platform ... were included with may web application vulnerability releases to show examples of vulnerable web sites. ... Tag. WordPress Core Metasploit Framework (MSF) WordPress Plugin SQL Injection (SQLi) Cross-Site Scripting (XSS) File Inclusion ... sequence[target.sequence],sizeof(buf)-1); printf([+] Sending Escape Sequences\n); do { if (send(sock, buf, strlen(buf), 0) , ...
Myc-DDK-tagged)-Human interferon, alpha-inducible protein 27 (IFI27), transcript variant 2 ... Sequence Data. ORF Nucleotide Sequence (showhide) >RC201952 ORF sequence. Red=Cloning site Blue=ORF Green=Tags(s). ... Protein Sequence (showhide) >RC201952 protein sequence. Red=Cloning site Green=Tags(s). ... IFI27 (tGFP-tagged) - Human interferon, alpha-inducible protein 27 (IFI27), transcript variant 2. * * 10 ug. 10 ug ...
KpnI restriction site was introduced at the C-terminus and used for polyhistidine tag (H9) fusion. The final construct was ... 1a) was synthesized de novo (Eurofins). The nucleotide sequence was optimized for Leishmania tarentolae expression with the ... coli using gene with an N-terminal 6ХHis-tag and upstream TEV-protease site cloned into pET28a(+) (Addgene plasmid #2006153). ... followed by the FLAG tag epitope (DYKDDDDK) and C-terminal 10×His tag were cloned into pcDNA3.1(-) at BamHI(5′) and HindIII(3 ...
Follow along with the video below to see how to install our site as a web app on your home screen. ... Sequence of Up-,Down-,Up-, repeat until.... Hi, Is this possible to detect this kind of sequence in 250k-750k row data. 1) ... Tags JavaScript is disabled. For a better experience, please enable JavaScript in your browser before proceeding. ... It may not display this or other websites correctly.. You should upgrade or use an alternative browser. ...
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and the corresponding mutation sites were shown in Table 2. His-tagged protein purification was performed by using Ni-NTA resin ... Although the sequence identities for Adx/Pdx and AdR/PdR are only 32.4% and 19.4% (Supplementary Fig. 10), respectively, Pdx ... All the P450s and RPs were expressed in E. coli (DE3) and purified via His-tag43. Typically, the cultures of recombinant cells ... The truncated form of Adx (4-108 amino acids) and AdR were expressed in pCwori and pET30a with a C-terminal His-tag to help ...
Tag: Potters Sequence. Congresswomans miracle baby survives fatal pregnancy August 7, 2013. Laymanfatal pregnancy, Jaime ... months after doctors diagnosed her with Potters Sequence, a fatal condition. The rare pre-natal disease causes impaired kidney ... Herrera Beutler, miracle, Potters Sequence, prayers WORLD News Service Washington Rep. Jaime Herrera Beutler and her husband ...
Suga, K., Mark Welch, D. B., Tanaka, Y., Sakakura, Y., & Hagiwara, A. (2007). "Analysis of expressed sequence tags of the ... "Coincidence of cleavage sites of intron endonuclease I-TevI and critical sequences of the host thymidylate synthase gene." J ... Kysela, D. T., Palacios, C., & Sogin, M. L. (2005). "Serial analysis of V6 ribosomal sequence tags (SARST-V6): a method for ... "Massively parallel tag sequencing reveals the complexity of anaerobic marine protistan communities." BMC Biol, 7, 72. doi: ...
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Characterisation and mapping of expressed sequence tags or ESTs to serve as anchored sequences tagged sites (STS). d. ... An examination of the content and distribution of repetitive DNA sequences to identify the location of such sequences within ... Link to main project webpage Link to European Plant Biotechnology Network description of the project. http://www.mpiz-koeln.mpg ... Systematic sequencing of cDNA clones derived from xylem and leaf tissue will produce a new generation of research tools for ...
sequence 1. sequence assembly 1. sequence tagged site 1. systems biology 1 ... http://beaconw.cs.manchester.ac.uk:8092/mcisb-web/ontology?wsdl 1 ...
The links to the Web sites in the following list focus on protein sequences. ... comScore Tag --,","enabled":true},{"pages":["all"],"location":"footer","script":",!--BEGIN QUALTRICS WEBSITE FEEDBACK SNIPPET ... "Bioinformatics Web Sites for Analyzing DNA/RNA Sequences","slug":"bioinformatics-web-sites-for-analyzing-dnarna-sequences"," ... "bioinformatics web sites for analyzing protein sequences","slug":"bioinformatics-web-sites-for-analyzing-protein-sequences"," ...
the sequences for the SacII site and 6x Histidine tag are underlined. ... the sequences for the EcoRI site and 6× Histidine tag are underlined. ... Based on our investigations, a signal peptide predicted at the N-terminal sequence of full-length ADN using SignalP 4.0 website ... Sequences of pLxEGFP-2A-ADN and pLxEGFP-m2A-ADN were confirmed by DNA sequencing with at least a two-fold overlap. ...
A targeted exome panel sequencing was performed by Next Generation Sequencing using the TrueSight One Sequencing Panel ( ... The complete human wild-type HA-tagged TRPM4 cDNA was cloned in a pcDNA4/TO vector. Mutants were obtained by in vitro ... mutagenesis using Q5 Site-Directed Mutagenesis kit (New England Biolabs, Ipswich, MA, USA). Mutant cDNA clones were then ... Illumina, San Diego, CA, USA). Sequence data were compared with the reference sequence of the human TRPM4 gene (MIM# 606936; ...
Classic CRISPR-Cas9 is a buddy tag team. One component, guide RNA (gRNA) is the bloodhound that hunts down the target DNA ... sequence. Once detected, Cas9 hones in on the site and efficiently cuts the double helix strands. The system was initially ... This Weeks Awesome Tech Stories From Around the Web (Through December 2). December 2, 2023. ...
Tagged Big Data, Cloud, Announcement, Investment, Series A, life sciences, Genomics, Duplex Sequencing, Cancer Detection ... This website uses cookies to improve your experience while you navigate through the website. Out of these, the cookies that are ... The result is a high-resolution reading of the DNA sequence, 10,000x more accurate than standard sequencing. Duplex Sequencing ... Tag: Duplex Sequencing. Our Investment in TwinStrand Biosciences, Leveraging Big Data And The Cloud To Improve Genome ...
With OneClickUpsell you can use tags to make different offers depending on what was ordered. Control the sequence of offers you ... Increase your average order value by adding post-purchase upsells to your checkout sequence will make you more money from the ...
The mutations were verified by DNA sequence analysis. In addition, we used the BacMan system for the transfection of GFP-tagged ... Site-directed mutagenesis of NEMO (serine to alanine) was performed using the QuikChange site-directed mutagenesis kit from ... We use cookies on our website to ensure you get the best experience.. Read more about our cookies here. ... First, we constructed wild-type and mutated constructs of NEMO/IKKγ tagged to GFP and visualized their presentation in living ...
Tags. amotl2 antibody bglii site bl21 cells bl21 de3 cdna plasmid corticosterone elisa kit co transfection protocol dna ... Tag: v5 tag sequence. Leptin modulates gene expression in the heart, cardiomyocytes and the adipose tissue thus mitigating LPS- ... Anti-anti-idiotypic (Ab3) antibodies that bind progesterone-11alpha-bovine serum albumin differ in their combining sites from ... antibodies that bind progesterone-11alpha-bovine serum albumin differ in their combining sites from antibodies raised directly ...
  • A sequence-tagged site (or STS) is a short (200 to 500 base pair) DNA sequence that has a single occurrence in the genome and whose location and base sequence are known. (wikipedia.org)
  • Sequence-tagged connectors: a sequence approach to mapping and scanning the human genome. (jcvi.org)
  • The sequence-tagged connector (STC) strategy proposes to generate sequence tags densely scattered (every 3.3 kilobases) across the human genome by arraying 450,000 bacterial artificial chromosomes (BACs) with randomly cleaved inserts, sequencing both ends of each, and preparing a restriction enzyme fingerprint of each. (jcvi.org)
  • By reiterating this "sequence-then-map by computer analysis against the STC database" strategy, a minimum tiling path of clones can be sequenced at a rate that is primarily limited by the sequencing throughput of individual genome centers. (jcvi.org)
  • This genome survey reveals numerous genes, genome-wide repeats, simple sequence repeats (potential genetic markers), and CpG islands (potential gene initiation sites). (jcvi.org)
  • Because the STC resource permits the easy integration of genetic, physical, gene, and sequence maps for chromosomes, it will be a powerful tool for the initial analysis of the human genome and other complex genomes. (jcvi.org)
  • The DNA is then replicated to enable sequencing on a standard genome sequencer - resulting in up to 6 TB of data per run - then imported to the TwinStrand cloud where error correction algorithms are employed. (madrona.com)
  • Rapid genomic sequencing includes both rapid genome sequencing (rGS) and rapid exome sequencing (rES). (cdc.gov)
  • Genome Sequencing in the Clinic - The question is not whether the glass is half-full or half-empty but whether or not the glass is the right tool. (cdc.gov)
  • Over the last decade, genetic testing has evolved from examining a few well-defined variants in one or a few genes to the capability to examine much of the human genome using next generation sequencing (NGS). (cdc.gov)
  • and rapidly determine all the DNA sequences in the genome that regulate the expression of a specific gene. (finchannel.com)
  • A key point of the reference genome is that it is not a single sequence. (scienceblogs.com)
  • Targeted sequencing of the S gene, using either NGS or Sanger sequencing, or whole genome sequencing using NGS will provide positive identification of Omicron. (who.int)
  • After subcloning the gene of interest into pSNAP-tag(m) as a fusion with the snap26m gene, the resulting plasmid can be used for stable or transient expression of the SNAP-tag fusion proteins in a suitable cell line. (neb.com)
  • The pQE TriSystem Vector allows for high-level expression of His-tagged proteins from a single vector containing three different expression systems. (qiagen.com)
  • SARS-CoV-2 Host Receptor Proteins Synonym ACE-2,ACEH,ACE2 Source Paguma larvata ACE2, His Tag (AC2-P5248) is. (proteomecommons.org)
  • Different proteins were known to have unique sequences, whereas it was thought that all DNA molecules have the same sequence. (proprofs.com)
  • Affinity tagging, a popular method for capturing or immobilizing proteins, has several incarnations, none more popular than polyhistidine tagging. (genengnews.com)
  • NTA and IDA fix transition metal ions to the matrix, where they complex polyhistidine sequences on proteins of interest. (genengnews.com)
  • A company specializing in IMAC resins for purifying polyHIS-tagged proteins is Cube Biotech. (genengnews.com)
  • Cube Biotech offers PureCube resins for the purification of His-tagged proteins. (genengnews.com)
  • Specifically, cullin-7 helps assemble a complex called an E3 ubiquitin ligase, which tags the unneeded proteins for degradation. (medlineplus.gov)
  • Mutations in the CUL7 or OBSL1 gene prevent the cullin-7 protein from bringing together the components of the E3 ubiquitin ligase complex, interfering with the process of tagging unneeded proteins for degradation. (medlineplus.gov)
  • The prediction of transcription factor binding sites in genomic sequences is in principle very useful to identify upstream regulatory factors. (lu.se)
  • To subclone the gene of interest into pSNAP-tag(m) fused to the N-terminus of the SNAP-tag use the available restriction sites ClaI , EcoRV (blunt) and EcoRI which are located upstream of the SNAP-tag. (neb.com)
  • Note: When fusing the gene of interest to the C-Terminus of the SNAP-tag, note that there is a stop codon between the XhoI and NotI sites, so SbfI, AscI, BamHI or XhoI must be used as the 5´ cloning site for your insert. (neb.com)
  • Design your PCR primers to include a sufficient overlap with the sequence of the gene you want to amplify. (neb.com)
  • This is only possible if the fusion partner has compatible sites adjacent to the gene of interest. (neb.com)
  • If subcloning of your gene of interest with the SNAP-tag does not work, reconfirm all the cloning steps (primer design, choice of restriction site, etc. (neb.com)
  • Alternatively try to subclone the SNAP-tag gene into an expression vector already containing your gene of interest. (neb.com)
  • The molecular sequence of this clone aligns with the gene accession number as a point of reference only. (origene.com)
  • However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. (origene.com)
  • Single pass sequencing of cDNA clones provides a rapid route to gene discovery and production of EST's (2). (le.ac.uk)
  • We developed TS-REX, a database/software system that supports the analysis of tissue and cell type-specific transcription factor-gene networks based on expressed sequence tag abundance of transcription factor-encoding genes in UniGene EST libraries. (lu.se)
  • The Arc gene, which contains remnants of a structural GAG retrotransposon sequence, produces a protein that self-assembles into capsid-like structures harboring Arc mRNA. (lu.se)
  • While nine spCas9 gene editing sites surround the Arc start codon, the accuracy of the editing was highly sequence-dependent, with only a single target resulting in an in-frame reporter integration. (lu.se)
  • 5104 /gene="ORF1" /locus_tag="NoVGII_gp1" /db_xref="GeneID:27042434" CDS 5. (cdc.gov)
  • 3028 /gene="ORF1" /locus_tag="NoVGII_gp1" /product="VPg" /note="For Southampton calicivirus, both N-terminal and C-terminal cleavage sites have been confirmed by direct sequencing. (cdc.gov)
  • Sequencing of these specimens identified the new variant of SARS CoV-2 B.1.1.529, now called Omicron which has over 30 mutations in the S gene. (who.int)
  • The specific goal of the thesis project is to develop and implement microfluidics coupled to microscopy for optical read out of gene sequences and protein localization. (lu.se)
  • The Centers for Disease Control and Prevention (CDC) cannot attest to the accuracy of a non-federal website. (cdc.gov)
  • Earlier this fall, the Blue Cross Blue Shield Technology Evaluation Center produced a report evaluating the clinical use of exome sequencing in the diagnosis of rare diseases. (cdc.gov)
  • That this report was even generated is remarkable, as it marks an appreciable level of penetration by exome sequencing into clinical care. (cdc.gov)
  • What's the "value" of exome sequencing in children with neurodevelopmental disorders? (cdc.gov)
  • Exome sequencing (ES) is increasingly used as part of the genetics evaluation of neurodevelopmental disorders, and acute illness in newborns of suspected genetic origin, among others. (cdc.gov)
  • char *esc_sequence[]= {'Escape Sequences', '\x1b'']2;Insecure? (exploit-db.com)
  • PCL Typeface list with escape sequences on the HP LaserJet 4L printer. (piclist.com)
  • They are used in shotgun sequencing, specifically to aid sequence assembly. (wikipedia.org)
  • You may also want to include a Stop codon at the C-Terminus of the fusion (in front of the downstream cloning site) in order to terminate translation at this position. (neb.com)
  • Direct cloning can also be used to make fusions with the SNAP-tag. (neb.com)
  • If all steps are confirmed as being correct, then re-try the cloning using different restriction sites. (neb.com)
  • Each captured specimen was tagged with voucher number and morphometric measurements were taken. (bvsalud.org)
  • Systematic sequencing of cDNA clones derived from xylem and leaf tissue will produce a new generation of research tools for conifer biology. (le.ac.uk)
  • An examination of the content and distribution of repetitive DNA sequences to identify the location of such sequences within specific chromosomes and their conservation across species of the Pinaceae family. (le.ac.uk)
  • Fluorescent in situ hybridisation (FISH) on metaphase chromosomes and on extended chromatin will be used to physically locate repetitive as well as single copy DNA sequences on conifer chromosomes and to integrate physical and genetic maps. (le.ac.uk)
  • If required, specific protease cleavage sites can be introduced into the linker peptide. (neb.com)
  • The STC resource, containing end sequences, fingerprints, and arrayed BACs, creates a map where the interrelationships of the individual BAC clones are resolved through their STCs as overlapping BAC clones are sequenced. (jcvi.org)
  • Once a seed or initiation BAC clone is sequenced, the minimum overlapping 5' and 3' BAC clones can be identified computationally and sequenced. (jcvi.org)
  • It also illustrates the power of the STC strategy for creating minimum tiling paths of BAC clones for large-scale genomic sequencing. (jcvi.org)
  • The DNA of Uromastyx hardwickii was amplified and sequenced using 16S rRNA primer set. (bvsalud.org)
  • The use of expression levels of transcription factor-encoding genes according to hierarchical anatomical classifications covering different tissues and cell types makes it possible to filter out irrelevant binding site predictions and to identify candidates of potential functional importance for further experimental testing. (lu.se)
  • The Palabras site has grown to include about 2000 short videos compiled from the original project in Buenos Aires, with additional footage from communities in Darfur, Sudan, San Francisco, California, and Kiel, Germany. (citris-uc.org)
  • The pQE-TriSystem vector contains CAG, T5, and p10 promoters that enable 6xHis-tagged protein expression in mammalian, E. coli , and baculovirus-infected insect cells, respectively (see figure pQE TriSystem. pQE TriSystem. "> pQE TriSystem ). (qiagen.com)
  • This clone was engineered to express the complete ORF with an expression tag. (origene.com)
  • Both of these tags improve fusion protein solubility and expression levels but exhibit immunogenicity for purposes of raising antiprotein antibodies. (genengnews.com)
  • On 26 November 2021, WHO on the advice of the Technical Advisory Group on SARS-CoV-2 Virus Evolution (TAG-VE) designated a new Variant of Concern (VOC), Omicron. (who.int)
  • Abstract During the present study, specimens were collected from selected sites of Cholistan desert and Kalabagh Game Reserve, Punjab province, Pakistan. (bvsalud.org)
  • The obtained DNA sequence has shown reliable and clear species identification. (bvsalud.org)
  • The reference sequence is identical to AB044366. (cdc.gov)
  • Scientific tags: Sequencing in tissues - Explore biological processes at the single base resolution with novel (micro-)fluidics. (lu.se)
  • In this project you wil participate in an interdisciplinary research team with the goal of developing state-of-the-art sequencing technologies inside biological tissues. (lu.se)
  • Further mutations: furin cleavage site RRAR modified to GSAG, K986P, V987P. (biovendor.com)
  • When STS loci contain genetic polymorphisms (e.g. simple sequence length polymorphisms, SSLPs, single nucleotide polymorphisms), they become valuable genetic markers, i.e. loci which can be used to distinguish individuals. (wikipedia.org)
  • We use cookies to distinguish you from other users and to provide you with a better experience on our websites. (cambridge.org)
  • Techniques exist that can identify 5-mC in genomic DNA, but the most commonly used method, bisulfite sequencing, is laborious and cannot distinguish between 5-mC from 5-hmC. (neb.com)
  • Over time, the term "dork" became shorthand for a search query that located sensitive information and "dorks" were included with may web application vulnerability releases to show examples of vulnerable web sites. (exploit-db.com)
  • The Palabras software allows people to easily tag, search, and sequence their footage. (citris-uc.org)
  • Visitors to the piece can use tags to search for clips of relevant material from their own and other communities. (citris-uc.org)
  • For fusions upstream of the SNAP-tag, ensure that a start codon is included. (neb.com)
  • For this reason they are useful for constructing genetic and physical maps from sequence data reported from many different laboratories. (wikipedia.org)
  • Hi, Is this possible to detect this kind of sequence in 250k-750k row data. (mrexcel.com)
  • Instead it is an assembly of consensus sequences that are designed to deal with variation in the human population and uncertainty in the data. (scienceblogs.com)
  • The relative prevalence of each mutation in Omicron sequences in not yet known as the sequencing data at the present time is limited. (who.int)
  • Segregating populations will be characterised with both single locus (microsatellites) and multi-locus (AFLPs) marker systems providing framework maps for the eventual location of cDNAs generated from single pass sequencing. (le.ac.uk)
  • DNA, randomly amplified polymorphic DNA (RAPD) locus sequence types that are not found in human GBS analysis, and multilocus sequence typing (MLST), have strains. (cdc.gov)
  • After trimming ambiguous bases, the obtained 16S rRNA fragment was 520 bp while 16S rRNA fragments aligned with closely matched sequence from NCBI comprised of 510 bp. (bvsalud.org)
  • Closely matched sequences of genus Uromastyx were retrieved from NCBI in blast searches. (bvsalud.org)
  • Since Homlisti has the One-Click Demo Importer, you can install the demo you want your site to resemble in a single click. (themeforest.net)
  • Control DNA substrates are synthetic 100 bp double stranded fragments containing a single MspI/HpaII site (CCGG). (neb.com)
  • A single looped tag line approximately 45 feet in length was in use. (cdc.gov)
  • Proponents argue that next generation sequencing (NGS) in acutely ill infants can deliver timely diagnostic results and enable life-changing clinical interventions. (cdc.gov)
  • Together, they have built an incredible foundation-contributing to more than 15 peer-reviewed scientific articles leveraging Duplex Sequencing and developing a portfolio of over 50 patents. (madrona.com)
  • NEB 10-beta Competent E. coli (High Efficiency) ( NEB #C3019 ) is recommended for propagating and subcloning this vector, unless the ClaI site will be used. (neb.com)
  • When using the ClaI site, dam - /dcm - Competent E. coli ( NEB #C2925 ) is recommended. (neb.com)
  • We do not evaluate or guarantee the accuracy of any content in this site. (lookfordiagnosis.com)
  • In my view, if all site owners and bloggers made excellent content material as you did, the web might be a lot more useful than ever before. (blackstarnews.com)
  • Choose a web site to get translated content where available and see local events and offers. (mathworks.com)
  • When students first look at an amino acid sequence, a long string of confusing letters, they often think those letters are part of a chain like an uncooked spaghetti noodle. (scienceblogs.com)
  • Other choices include calmodulin-binding peptide, Strep-tag (oxtapeptide binding to streptavidini), chitin-binding domain, and FLAG-tag (another octapeptide that binds to anti-FLAG mAbs). (genengnews.com)
  • TwinStrand's "Duplex Sequencing" technology uniquely tags each strand of billions of individual DNA molecules with a chemical GUID. (madrona.com)
  • To enable the tracking of Arc molecules from individual neurons in vivo, we devised an adeno-associated virus (AAV) mediated approach to tag the N-terminal of the mouse Arc protein with a fluorescent reporter using CRISPR/Cas9 homologous. (lu.se)
  • To enable the tracking of Arc molecules from individual neurons in vivo, we devised an adeno-associated virus (AAV) mediated approach to tag the N-terminal of the mouse Arc protein with a fluorescent reporter using CRISPR/Cas9 homologous independent targeted integration (HITI). (lu.se)
  • He thanked CDC and KTL for their support in providing accurate genomic sequencing results of polioviruses in a timely manner, as this greatly facilitated planning and targeting the polio eradication activities. (who.int)
  • The three fragments are identical except for modification of the internal C in this site. (neb.com)
  • Anti-anti-idiotypic (Ab3) antibodies that bind progesterone-11alpha-bovine serum albumin differ in their combining sites from antibodies raised directly against the antigen. (aetox.com)
  • One component, guide RNA (gRNA) is the bloodhound that hunts down the target DNA sequence. (singularityhub.com)
  • Turturo C, Friscina A, Gaubert S, Jacquemond M, Thompson JR, Tepfer M (in press) Evaluation of the potential risks associated with recombination in transgenic plants expressing viral sequences. (cambridge.org)
  • These homepage demos are fully equipped with pages and elements that a real estate listing website needs. (themeforest.net)
  • You can allow the members permission to all categories or the entire site and allow them a higher number of ads. (themeforest.net)
  • We show that a sequence coding for mCherry can successfully be knocked in at the 5′ end of the Arc open reading frame. (lu.se)
  • We also offer solutions for automation, site-directed mutagenesis, as well as your favorite restriction enzyme, ligase or competent cell products. (neb.com)
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  • Disclaimer: The information and views set out on this website are those of the author(s) and do not necessarily reflect the opinion of the Parties to the WHO Framework Convention on Tobacco Control (WHO FCTC), the Parties to the Protocol to Eliminate Illicit Trade in Tobacco Products, or the Secretariat of the WHO FCTC. (who.int)