Sequence Analysis, DNA: A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Sequence Analysis: A multistage process that includes the determination of a sequence (protein, carbohydrate, etc.), its fragmentation and analysis, and the interpretation of the resulting sequence information.Phylogeny: The relationships of groups of organisms as reflected by their genetic makeup.Cloning, Molecular: The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.RNA, Ribosomal, 16S: Constituent of 30S subunit prokaryotic ribosomes containing 1600 nucleotides and 21 proteins. 16S rRNA is involved in initiation of polypeptide synthesis.DNA, Bacterial: Deoxyribonucleic acid that makes up the genetic material of bacteria.Sequence Homology, Nucleic Acid: The sequential correspondence of nucleotides in one nucleic acid molecule with those of another nucleic acid molecule. Sequence homology is an indication of the genetic relatedness of different organisms and gene function.DNA, Ribosomal: DNA sequences encoding RIBOSOMAL RNA and the segments of DNA separating the individual ribosomal RNA genes, referred to as RIBOSOMAL SPACER DNA.Sequence Homology, Amino Acid: The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.Sequence Alignment: The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.Genes, Bacterial: The functional hereditary units of BACTERIA.Polymerase Chain Reaction: In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.Restriction Mapping: Use of restriction endonucleases to analyze and generate a physical map of genomes, genes, or other segments of DNA.Base Composition: The relative amounts of the PURINES and PYRIMIDINES in a nucleic acid.Bacterial Proteins: Proteins found in any species of bacterium.Open Reading Frames: A sequence of successive nucleotide triplets that are read as CODONS specifying AMINO ACIDS and begin with an INITIATOR CODON and end with a stop codon (CODON, TERMINATOR).Genes, rRNA: Genes, found in both prokaryotes and eukaryotes, which are transcribed to produce the RNA which is incorporated into RIBOSOMES. Prokaryotic rRNA genes are usually found in OPERONS dispersed throughout the GENOME, whereas eukaryotic rRNA genes are clustered, multicistronic transcriptional units.Nucleic Acid Hybridization: Widely used technique which exploits the ability of complementary sequences in single-stranded DNAs or RNAs to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (Kendrew, Encyclopedia of Molecular Biology, 1994, p503)Sequence Analysis, Protein: A process that includes the determination of AMINO ACID SEQUENCE of a protein (or peptide, oligopeptide or peptide fragment) and the information analysis of the sequence.DNA: A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).Mutation: Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.Species Specificity: The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.Bacterial Typing Techniques: Procedures for identifying types and strains of bacteria. The most frequently employed typing systems are BACTERIOPHAGE TYPING and SEROTYPING as well as bacteriocin typing and biotyping.Escherichia coli: A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.DNA, Complementary: Single-stranded complementary DNA synthesized from an RNA template by the action of RNA-dependent DNA polymerase. cDNA (i.e., complementary DNA, not circular DNA, not C-DNA) is used in a variety of molecular cloning experiments as well as serving as a specific hybridization probe.Plasmids: Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.DNA Primers: Short sequences (generally about 10 base pairs) of DNA that are complementary to sequences of messenger RNA and allow reverse transcriptases to start copying the adjacent sequences of mRNA. Primers are used extensively in genetic and molecular biology techniques.Multigene Family: A set of genes descended by duplication and variation from some ancestral gene. Such genes may be clustered together on the same chromosome or dispersed on different chromosomes. Examples of multigene families include those that encode the hemoglobins, immunoglobulins, histocompatibility antigens, actins, tubulins, keratins, collagens, heat shock proteins, salivary glue proteins, chorion proteins, cuticle proteins, yolk proteins, and phaseolins, as well as histones, ribosomal RNA, and transfer RNA genes. The latter three are examples of reiterated genes, where hundreds of identical genes are present in a tandem array. (King & Stanfield, A Dictionary of Genetics, 4th ed)Genes: A category of nucleic acid sequences that function as units of heredity and which code for the basic instructions for the development, reproduction, and maintenance of organisms.DNA, Ribosomal Spacer: The intergenic DNA segments that are between the ribosomal RNA genes (internal transcribed spacers) and between the tandemly repeated units of rDNA (external transcribed spacers and nontranscribed spacers).RNA, Bacterial: Ribonucleic acid in bacteria having regulatory and catalytic roles as well as involvement in protein synthesis.Genetic Variation: Genotypic differences observed among individuals in a population.RNA, Messenger: RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.Chromosome Mapping: Any method used for determining the location of and relative distances between genes on a chromosome.Sequence Homology: The degree of similarity between sequences. Studies of AMINO ACID SEQUENCE HOMOLOGY and NUCLEIC ACID SEQUENCE HOMOLOGY provide useful information about the genetic relatedness of genes, gene products, and species.Blotting, Southern: A method (first developed by E.M. Southern) for detection of DNA that has been electrophoretically separated and immobilized by blotting on nitrocellulose or other type of paper or nylon membrane followed by hybridization with labeled NUCLEIC ACID PROBES.Gene Library: A large collection of DNA fragments cloned (CLONING, MOLECULAR) from a given organism, tissue, organ, or cell type. It may contain complete genomic sequences (GENOMIC LIBRARY) or complementary DNA sequences, the latter being formed from messenger RNA and lacking intron sequences.Transcription, Genetic: The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.Phenotype: The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment.Soil Microbiology: The presence of bacteria, viruses, and fungi in the soil. This term is not restricted to pathogenic organisms.Repetitive Sequences, Nucleic Acid: Sequences of DNA or RNA that occur in multiple copies. There are several types: INTERSPERSED REPETITIVE SEQUENCES are copies of transposable elements (DNA TRANSPOSABLE ELEMENTS or RETROELEMENTS) dispersed throughout the genome. TERMINAL REPEAT SEQUENCES flank both ends of another sequence, for example, the long terminal repeats (LTRs) on RETROVIRUSES. Variations may be direct repeats, those occurring in the same direction, or inverted repeats, those opposite to each other in direction. TANDEM REPEAT SEQUENCES are copies which lie adjacent to each other, direct or inverted (INVERTED REPEAT SEQUENCES).Genome, Viral: The complete genetic complement contained in a DNA or RNA molecule in a virus.Molecular Weight: The sum of the weight of all the atoms in a molecule.DNA Restriction Enzymes: Enzymes that are part of the restriction-modification systems. They catalyze the endonucleolytic cleavage of DNA sequences which lack the species-specific methylation pattern in the host cell's DNA. Cleavage yields random or specific double-stranded fragments with terminal 5'-phosphates. The function of restriction enzymes is to destroy any foreign DNA that invades the host cell. Most have been studied in bacterial systems, but a few have been found in eukaryotic organisms. They are also used as tools for the systematic dissection and mapping of chromosomes, in the determination of base sequences of DNAs, and have made it possible to splice and recombine genes from one organism into the genome of another. EC 3.21.1.Sequence Analysis, RNA: A multistage process that includes cloning, physical mapping, subcloning, sequencing, and information analysis of an RNA SEQUENCE.Conserved Sequence: A sequence of amino acids in a polypeptide or of nucleotides in DNA or RNA that is similar across multiple species. A known set of conserved sequences is represented by a CONSENSUS SEQUENCE. AMINO ACID MOTIFS are often composed of conserved sequences.DNA Transposable Elements: Discrete segments of DNA which can excise and reintegrate to another site in the genome. Most are inactive, i.e., have not been found to exist outside the integrated state. DNA transposable elements include bacterial IS (insertion sequence) elements, Tn elements, the maize controlling elements Ac and Ds, Drosophila P, gypsy, and pogo elements, the human Tigger elements and the Tc and mariner elements which are found throughout the animal kingdom.DNA, Viral: Deoxyribonucleic acid that makes up the genetic material of viruses.Cluster Analysis: A set of statistical methods used to group variables or observations into strongly inter-related subgroups. In epidemiology, it may be used to analyze a closely grouped series of events or cases of disease or other health-related phenomenon with well-defined distribution patterns in relation to time or place or both.Evolution, Molecular: The process of cumulative change at the level of DNA; RNA; and PROTEINS, over successive generations.Exons: The parts of a transcript of a split GENE remaining after the INTRONS are removed. They are spliced together to become a MESSENGER RNA or other functional RNA.DNA, Fungal: Deoxyribonucleic acid that makes up the genetic material of fungi.Genes, Viral: The functional hereditary units of VIRUSES.Electrophoresis, Polyacrylamide Gel: Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.Genotype: The genetic constitution of the individual, comprising the ALLELES present at each GENETIC LOCUS.DNA Mutational Analysis: Biochemical identification of mutational changes in a nucleotide sequence.Genetic Complementation Test: A test used to determine whether or not complementation (compensation in the form of dominance) will occur in a cell with a given mutant phenotype when another mutant genome, encoding the same mutant phenotype, is introduced into that cell.Fatty Acids: Organic, monobasic acids derived from hydrocarbons by the equivalent of oxidation of a methyl group to an alcohol, aldehyde, and then acid. Fatty acids are saturated and unsaturated (FATTY ACIDS, UNSATURATED). (Grant & Hackh's Chemical Dictionary, 5th ed)Blotting, Northern: Detection of RNA that has been electrophoretically separated and immobilized by blotting on nitrocellulose or other type of paper or nylon membrane followed by hybridization with labeled NUCLEIC ACID PROBES.Polymorphism, Restriction Fragment Length: Variation occurring within a species in the presence or length of DNA fragment generated by a specific endonuclease at a specific site in the genome. Such variations are generated by mutations that create or abolish recognition sites for these enzymes or change the length of the fragment.Mannose-Binding Lectins: A subclass of lectins that are specific for CARBOHYDRATES that contain MANNOSE.Peptide Fragments: Partial proteins formed by partial hydrolysis of complete proteins or generated through PROTEIN ENGINEERING techniques.Binding Sites: The parts of a macromolecule that directly participate in its specific combination with another molecule.Genomic Library: A form of GENE LIBRARY containing the complete DNA sequences present in the genome of a given organism. It contrasts with a cDNA library which contains only sequences utilized in protein coding (lacking introns).Promoter Regions, Genetic: DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.Codon: A set of three nucleotides in a protein coding sequence that specifies individual amino acids or a termination signal (CODON, TERMINATOR). Most codons are universal, but some organisms do not produce the transfer RNAs (RNA, TRANSFER) complementary to all codons. These codons are referred to as unassigned codons (CODONS, NONSENSE).RNA, Viral: Ribonucleic acid that makes up the genetic material of viruses.Chromatography, High Pressure Liquid: Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed.Cardiac Output: The volume of BLOOD passing through the HEART per unit of time. It is usually expressed as liters (volume) per minute so as not to be confused with STROKE VOLUME (volume per beat).Recombinant Proteins: Proteins prepared by recombinant DNA technology.Oligonucleotide Probes: Synthetic or natural oligonucleotides used in hybridization studies in order to identify and study specific nucleic acid fragments, e.g., DNA segments near or within a specific gene locus or gene. The probe hybridizes with a specific mRNA, if present. Conventional techniques used for testing for the hybridization product include dot blot assays, Southern blot assays, and DNA:RNA hybrid-specific antibody tests. Conventional labels for the probe include the radioisotope labels 32P and 125I and the chemical label biotin.Cattle: Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.Operon: In bacteria, a group of metabolically related genes, with a common promoter, whose transcription into a single polycistronic MESSENGER RNA is under the control of an OPERATOR REGION.Cell Line: Established cell cultures that have the potential to propagate indefinitely.Viral Proteins: Proteins found in any species of virus.Point Mutation: A mutation caused by the substitution of one nucleotide for another. This results in the DNA molecule having a change in a single base pair.Seawater: The salinated water of OCEANS AND SEAS that provides habitat for marine organisms.Alleles: Variant forms of the same gene, occupying the same locus on homologous CHROMOSOMES, and governing the variants in production of the same gene product.Gene Expression: The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.Actinomycetales: An order of gram-positive, primarily aerobic BACTERIA that tend to form branching filaments.Multilocus Sequence Typing: Direct nucleotide sequencing of gene fragments from multiple housekeeping genes for the purpose of phylogenetic analysis, organism identification, and typing of species, strain, serovar, or other distinguishable phylogenetic level.Recombination, Genetic: Production of new arrangements of DNA by various mechanisms such as assortment and segregation, CROSSING OVER; GENE CONVERSION; GENETIC TRANSFORMATION; GENETIC CONJUGATION; GENETIC TRANSDUCTION; or mixed infection of viruses.Amino Acids: Organic compounds that generally contain an amino (-NH2) and a carboxyl (-COOH) group. Twenty alpha-amino acids are the subunits which are polymerized to form proteins.Gene Expression Regulation, Bacterial: Any of the processes by which cytoplasmic or intercellular factors influence the differential control of gene action in bacteria.Genes, Fungal: The functional hereditary units of FUNGI.Trypsin: A serine endopeptidase that is formed from TRYPSINOGEN in the pancreas. It is converted into its active form by ENTEROPEPTIDASE in the small intestine. It catalyzes hydrolysis of the carboxyl group of either arginine or lysine. EC 3.4.21.4.Consensus Sequence: A theoretical representative nucleotide or amino acid sequence in which each nucleotide or amino acid is the one which occurs most frequently at that site in the different sequences which occur in nature. The phrase also refers to an actual sequence which approximates the theoretical consensus. A known CONSERVED SEQUENCE set is represented by a consensus sequence. Commonly observed supersecondary protein structures (AMINO ACID MOTIFS) are often formed by conserved sequences.Reverse Transcriptase Polymerase Chain Reaction: A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.Cyanogen Bromide: Cyanogen bromide (CNBr). A compound used in molecular biology to digest some proteins and as a coupling reagent for phosphoroamidate or pyrophosphate internucleotide bonds in DNA duplexes.RNA: A polynucleotide consisting essentially of chains with a repeating backbone of phosphate and ribose units to which nitrogenous bases are attached. RNA is unique among biological macromolecules in that it can encode genetic information, serve as an abundant structural component of cells, and also possesses catalytic activity. (Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)Alphaproteobacteria: A class in the phylum PROTEOBACTERIA comprised mostly of two major phenotypes: purple non-sulfur bacteria and aerobic bacteriochlorophyll-containing bacteria.Nucleic Acid Conformation: The spatial arrangement of the atoms of a nucleic acid or polynucleotide that results in its characteristic 3-dimensional shape.Water Microbiology: The presence of bacteria, viruses, and fungi in water. This term is not restricted to pathogenic organisms.Proteins: Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.Geologic Sediments: A mass of organic or inorganic solid fragmented material, or the solid fragment itself, that comes from the weathering of rock and is carried by, suspended in, or dropped by air, water, or ice. It refers also to a mass that is accumulated by any other natural agent and that forms in layers on the earth's surface, such as sand, gravel, silt, mud, fill, or loess. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed, p1689)Virulence: The degree of pathogenicity within a group or species of microorganisms or viruses as indicated by case fatality rates and/or the ability of the organism to invade the tissues of the host. The pathogenic capacity of an organism is determined by its VIRULENCE FACTORS.Protein Conformation: The characteristic 3-dimensional shape of a protein, including the secondary, supersecondary (motifs), tertiary (domains) and quaternary structure of the peptide chain. PROTEIN STRUCTURE, QUATERNARY describes the conformation assumed by multimeric proteins (aggregates of more than one polypeptide chain).Protein Biosynthesis: The biosynthesis of PEPTIDES and PROTEINS on RIBOSOMES, directed by MESSENGER RNA, via TRANSFER RNA that is charged with standard proteinogenic AMINO ACIDS.RNA, Ribosomal, 23S: Constituent of 50S subunit of prokaryotic ribosomes containing about 3200 nucleotides. 23S rRNA is involved in the initiation of polypeptide synthesis.Substrate Specificity: A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.Pedigree: The record of descent or ancestry, particularly of a particular condition or trait, indicating individual family members, their relationships, and their status with respect to the trait or condition.Sequence Deletion: Deletion of sequences of nucleic acids from the genetic material of an individual.Genome, Bacterial: The genetic complement of a BACTERIA as represented in its DNA.Chickens: Common name for the species Gallus gallus, the domestic fowl, in the family Phasianidae, order GALLIFORMES. It is descended from the red jungle fowl of SOUTHEAST ASIA.DNA, Recombinant: Biologically active DNA which has been formed by the in vitro joining of segments of DNA from different sources. It includes the recombination joint or edge of a heteroduplex region where two recombining DNA molecules are connected.Membrane Proteins: Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors.Bacteria: One of the three domains of life (the others being Eukarya and ARCHAEA), also called Eubacteria. They are unicellular prokaryotic microorganisms which generally possess rigid cell walls, multiply by cell division, and exhibit three principal forms: round or coccal, rodlike or bacillary, and spiral or spirochetal. Bacteria can be classified by their response to OXYGEN: aerobic, anaerobic, or facultatively anaerobic; by the mode by which they obtain their energy: chemotrophy (via chemical reaction) or PHOTOTROPHY (via light reaction); for chemotrophs by their source of chemical energy: CHEMOLITHOTROPHY (from inorganic compounds) or chemoorganotrophy (from organic compounds); and by their source for CARBON; NITROGEN; etc.; HETEROTROPHY (from organic sources) or AUTOTROPHY (from CARBON DIOXIDE). They can also be classified by whether or not they stain (based on the structure of their CELL WALLS) with CRYSTAL VIOLET dye: gram-negative or gram-positive.Plant Proteins: Proteins found in plants (flowers, herbs, shrubs, trees, etc.). The concept does not include proteins found in vegetables for which VEGETABLE PROTEINS is available.Protein PrecursorsPolymorphism, Genetic: The regular and simultaneous occurrence in a single interbreeding population of two or more discontinuous genotypes. The concept includes differences in genotypes ranging in size from a single nucleotide site (POLYMORPHISM, SINGLE NUCLEOTIDE) to large nucleotide sequences visible at a chromosomal level.Software: Sequential operating programs and data which instruct the functioning of a digital computer.Protein Structure, Tertiary: The level of protein structure in which combinations of secondary protein structures (alpha helices, beta sheets, loop regions, and motifs) pack together to form folded shapes called domains. Disulfide bridges between cysteines in two different parts of the polypeptide chain along with other interactions between the chains play a role in the formation and stabilization of tertiary structure. Small proteins usually consist of only one domain but larger proteins may contain a number of domains connected by segments of polypeptide chain which lack regular secondary structure.Streptomyces: A genus of bacteria that form a nonfragmented aerial mycelium. Many species have been identified with some being pathogenic. This genus is responsible for producing a majority of the ANTI-BACTERIAL AGENTS of practical value.Models, Molecular: Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures.RNA, Ribosomal, 5.8S: Constituent of the 60S subunit of eukaryotic ribosomes. 5.8S rRNA is involved in the initiation of polypeptide synthesis in eukaryotes.Plants: Multicellular, eukaryotic life forms of kingdom Plantae (sensu lato), comprising the VIRIDIPLANTAE; RHODOPHYTA; and GLAUCOPHYTA; all of which acquired chloroplasts by direct endosymbiosis of CYANOBACTERIA. They are characterized by a mainly photosynthetic mode of nutrition; essentially unlimited growth at localized regions of cell divisions (MERISTEMS); cellulose within cells providing rigidity; the absence of organs of locomotion; absence of nervous and sensory systems; and an alternation of haploid and diploid generations.Korea: Former kingdom, located on Korea Peninsula between Sea of Japan and Yellow Sea on east coast of Asia. In 1948, the kingdom ceased and two independent countries were formed, divided by the 38th parallel.Mutagenesis: Process of generating a genetic MUTATION. It may occur spontaneously or be induced by MUTAGENS.Biological Evolution: The process of cumulative change over successive generations through which organisms acquire their distinguishing morphological and physiological characteristics.Carrier Proteins: Transport proteins that carry specific substances in the blood or across cell membranes.Genes, Regulator: Genes which regulate or circumscribe the activity of other genes; specifically, genes which code for PROTEINS or RNAs which have GENE EXPRESSION REGULATION functions.Gammaproteobacteria: A group of the proteobacteria comprised of facultatively anaerobic and fermentative gram-negative bacteria.Gene Deletion: A genetic rearrangement through loss of segments of DNA or RNA, bringing sequences which are normally separated into close proximity. This deletion may be detected using cytogenetic techniques and can also be inferred from the phenotype, indicating a deletion at one specific locus.Oligodeoxyribonucleotides: A group of deoxyribonucleotides (up to 12) in which the phosphate residues of each deoxyribonucleotide act as bridges in forming diester linkages between the deoxyribose moieties.Swine: Any of various animals that constitute the family Suidae and comprise stout-bodied, short-legged omnivorous mammals with thick skin, usually covered with coarse bristles, a rather long mobile snout, and small tail. Included are the genera Babyrousa, Phacochoerus (wart hogs), and Sus, the latter containing the domestic pig (see SUS SCROFA).Religion and SexVitamin B 12 Deficiency: A nutritional condition produced by a deficiency of VITAMIN B 12 in the diet, characterized by megaloblastic anemia. Since vitamin B 12 is not present in plants, humans have obtained their supply from animal products, from multivitamin supplements in the form of pills, and as additives to food preparations. A wide variety of neuropsychiatric abnormalities is also seen in vitamin B 12 deficiency and appears to be due to an undefined defect involving myelin synthesis. (From Cecil Textbook of Medicine, 19th ed, p848)Computational Biology: A field of biology concerned with the development of techniques for the collection and manipulation of biological data, and the use of such data to make biological discoveries or predictions. This field encompasses all computational methods and theories for solving biological problems including manipulation of models and datasets.DNA, Plant: Deoxyribonucleic acid that makes up the genetic material of plants.Gene Expression Regulation: Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.RNA, Ribosomal: The most abundant form of RNA. Together with proteins, it forms the ribosomes, playing a structural role and also a role in ribosomal binding of mRNA and tRNAs. Individual chains are conventionally designated by their sedimentation coefficients. In eukaryotes, four large chains exist, synthesized in the nucleolus and constituting about 50% of the ribosome. (Dorland, 28th ed)Bacterial Outer Membrane Proteins: Proteins isolated from the outer membrane of Gram-negative bacteria.Transcription Factors: Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process.Poly A: A group of adenine ribonucleotides in which the phosphate residues of each adenine ribonucleotide act as bridges in forming diester linkages between the ribose moieties.Plant Diseases: Diseases of plants.Temperature: The property of objects that determines the direction of heat flow when they are placed in direct thermal contact. The temperature is the energy of microscopic motions (vibrational and translational) of the particles of atoms.Gene Order: The sequential location of genes on a chromosome.Peptide Mapping: Analysis of PEPTIDES that are generated from the digestion or fragmentation of a protein or mixture of PROTEINS, by ELECTROPHORESIS; CHROMATOGRAPHY; or MASS SPECTROMETRY. The resulting peptide fingerprints are analyzed for a variety of purposes including the identification of the proteins in a sample, GENETIC POLYMORPHISMS, patterns of gene expression, and patterns diagnostic for diseases.DNA-Binding Proteins: Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases.Pseudomonas: A genus of gram-negative, aerobic, rod-shaped bacteria widely distributed in nature. Some species are pathogenic for humans, animals, and plants.RNA, Ribosomal, 18S: Constituent of the 40S subunit of eukaryotic ribosomes. 18S rRNA is involved in the initiation of polypeptide synthesis in eukaryotes.Kinetics: The rate dynamics in chemical or physical systems.Saccharomyces cerevisiae: A species of the genus SACCHAROMYCES, family Saccharomycetaceae, order Saccharomycetales, known as "baker's" or "brewer's" yeast. The dried form is used as a dietary supplement.DNA Probes: Species- or subspecies-specific DNA (including COMPLEMENTARY DNA; conserved genes, whole chromosomes, or whole genomes) used in hybridization studies in order to identify microorganisms, to measure DNA-DNA homologies, to group subspecies, etc. The DNA probe hybridizes with a specific mRNA, if present. Conventional techniques used for testing for the hybridization product include dot blot assays, Southern blot assays, and DNA:RNA hybrid-specific antibody tests. Conventional labels for the DNA probe include the radioisotope labels 32P and 125I and the chemical label biotin. The use of DNA probes provides a specific, sensitive, rapid, and inexpensive replacement for cell culture techniques for diagnosing infections.Peptides: Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.Mycological Typing Techniques: Procedures for identifying types and strains of fungi.China: A country spanning from central Asia to the Pacific Ocean.Capsid Proteins: Proteins that form the CAPSID of VIRUSES.DNA Fingerprinting: A technique for identifying individuals of a species that is based on the uniqueness of their DNA sequence. Uniqueness is determined by identifying which combination of allelic variations occur in the individual at a statistically relevant number of different loci. In forensic studies, RESTRICTION FRAGMENT LENGTH POLYMORPHISM of multiple, highly polymorphic VNTR LOCI or MICROSATELLITE REPEAT loci are analyzed. The number of loci used for the profile depends on the ALLELE FREQUENCY in the population.RNA Splicing: The ultimate exclusion of nonsense sequences or intervening sequences (introns) before the final RNA transcript is sent to the cytoplasm.Alternative Splicing: A process whereby multiple RNA transcripts are generated from a single gene. Alternative splicing involves the splicing together of other possible sets of EXONS during the processing of some, but not all, transcripts of the gene. Thus a particular exon may be connected to any one of several alternative exons to form a mature RNA. The alternative forms of mature MESSENGER RNA produce PROTEIN ISOFORMS in which one part of the isoforms is common while the other parts are different.Cosmids: Plasmids containing at least one cos (cohesive-end site) of PHAGE LAMBDA. They are used as cloning vehicles.Quinones: Hydrocarbon rings which contain two ketone moieties in any position. They can be substituted in any position except at the ketone groups.Mutation, Missense: A mutation in which a codon is mutated to one directing the incorporation of a different amino acid. This substitution may result in an inactive or unstable product. (From A Dictionary of Genetics, King & Stansfield, 5th ed)Recombinant Fusion Proteins: Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.Fungal Proteins: Proteins found in any species of fungus.Polymorphism, Single-Stranded Conformational: Variation in a population's DNA sequence that is detected by determining alterations in the conformation of denatured DNA fragments. Denatured DNA fragments are allowed to renature under conditions that prevent the formation of double-stranded DNA and allow secondary structure to form in single stranded fragments. These fragments are then run through polyacrylamide gels to detect variations in the secondary structure that is manifested as an alteration in migration through the gels.Protein Binding: The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.Fresh Water: Water containing no significant amounts of salts, such as water from RIVERS and LAKES.Escherichia coli Proteins: Proteins obtained from ESCHERICHIA COLI.Viral Structural Proteins: Viral proteins that are components of the mature assembled VIRUS PARTICLES. They may include nucleocapsid core proteins (gag proteins), enzymes packaged within the virus particle (pol proteins), and membrane components (env proteins). These do not include the proteins encoded in the VIRAL GENOME that are produced in infected cells but which are not packaged in the mature virus particle,i.e. the so called non-structural proteins (VIRAL NONSTRUCTURAL PROTEINS).Prevotella: A genus of gram-negative, anaerobic, nonsporeforming, nonmotile rods. Organisms of this genus had originally been classified as members of the BACTEROIDES genus but overwhelming biochemical and chemical findings in 1990 indicated the need to separate them from other Bacteroides species, and hence, this new genus was established.Protein Structure, Secondary: The level of protein structure in which regular hydrogen-bond interactions within contiguous stretches of polypeptide chain give rise to alpha helices, beta strands (which align to form beta sheets) or other types of coils. This is the first folding level of protein conformation.Reading Frames: The three possible sequences of CODONS by which GENETIC TRANSLATION may occur from one nucleotide sequence. A segment of mRNA 5'AUCCGA3' could be translated as 5'AUC.. or 5'UCC.. or 5'CCG.., depending on the location of the START CODON.Bacillus: A genus of BACILLACEAE that are spore-forming, rod-shaped cells. Most species are saprophytic soil forms with only a few species being pathogenic.Frameshift Mutation: A type of mutation in which a number of NUCLEOTIDES deleted from or inserted into a protein coding sequence is not divisible by three, thereby causing an alteration in the READING FRAMES of the entire coding sequence downstream of the mutation. These mutations may be induced by certain types of MUTAGENS or may occur spontaneously.Endopeptidases: A subclass of PEPTIDE HYDROLASES that catalyze the internal cleavage of PEPTIDES or PROTEINS.Models, Genetic: Theoretical representations that simulate the behavior or activity of genetic processes or phenomena. They include the use of mathematical equations, computers, and other electronic equipment.Regulatory Sequences, Nucleic Acid: Nucleic acid sequences involved in regulating the expression of genes.Mutagenesis, Site-Directed: Genetically engineered MUTAGENESIS at a specific site in the DNA molecule that introduces a base substitution, or an insertion or deletion.Blotting, Western: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.Chromatography, Ion Exchange: Separation technique in which the stationary phase consists of ion exchange resins. The resins contain loosely held small ions that easily exchange places with other small ions of like charge present in solutions washed over the resins.Capsid: The outer protein protective shell of a virus, which protects the viral nucleic acid.KazakhstanAerobiosis: Life or metabolic reactions occurring in an environment containing oxygen.Transfection: The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.Pseudogenes: Genes bearing close resemblance to known genes at different loci, but rendered non-functional by additions or deletions in structure that prevent normal transcription or translation. When lacking introns and containing a poly-A segment near the downstream end (as a result of reverse copying from processed nuclear RNA into double-stranded DNA), they are called processed genes.Corynebacterium: A genus of asporogenous bacteria that is widely distributed in nature. Its organisms appear as straight to slightly curved rods and are known to be human and animal parasites and pathogens.Genes, Plant: The functional hereditary units of PLANTS.DNA, Mitochondrial: Double-stranded DNA of MITOCHONDRIA. In eukaryotes, the mitochondrial GENOME is circular and codes for ribosomal RNAs, transfer RNAs, and about 10 proteins.Serine Endopeptidases: Any member of the group of ENDOPEPTIDASES containing at the active site a serine residue involved in catalysis.Chromosomes, Artificial, Bacterial: DNA constructs that are composed of, at least, a REPLICATION ORIGIN, for successful replication, propagation to and maintenance as an extra chromosome in bacteria. In addition, they can carry large amounts (about 200 kilobases) of other sequence for a variety of bioengineering purposes.DNA, Intergenic: Any of the DNA in between gene-coding DNA, including untranslated regions, 5' and 3' flanking regions, INTRONS, non-functional pseudogenes, and non-functional repetitive sequences. This DNA may or may not encode regulatory functions.Amino Acid Motifs: Commonly observed structural components of proteins formed by simple combinations of adjacent secondary structures. A commonly observed structure may be composed of a CONSERVED SEQUENCE which can be represented by a CONSENSUS SEQUENCE.Macromolecular Substances: Compounds and molecular complexes that consist of very large numbers of atoms and are generally over 500 kDa in size. In biological systems macromolecular substances usually can be visualized using ELECTRON MICROSCOPY and are distinguished from ORGANELLES by the lack of a membrane structure.Hot Springs: Habitat of hot water naturally heated by underlying geologic processes. Surface hot springs have been used for BALNEOLOGY. Underwater hot springs are called HYDROTHERMAL VENTS.Chromosomes, Bacterial: Structures within the nucleus of bacterial cells consisting of or containing DNA, which carry genetic information essential to the cell.DNA, Protozoan: Deoxyribonucleic acid that makes up the genetic material of protozoa.Genome: The genetic complement of an organism, including all of its GENES, as represented in its DNA, or in some cases, its RNA.Sodium Chloride: A ubiquitous sodium salt that is commonly used to season food.Hydrogen-Ion Concentration: The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH = log 1/2[1/(H+)], where (H+) is the hydrogen ion concentration in gram equivalents per liter of solution. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)Chromosome Deletion: Actual loss of portion of a chromosome.Sewage: Refuse liquid or waste matter carried off by sewers.DNA, Archaeal: Deoxyribonucleic acid that makes up the genetic material of archaea.Rabbits: The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.Structure-Activity Relationship: The relationship between the chemical structure of a compound and its biological or pharmacological activity. Compounds are often classed together because they have structural characteristics in common including shape, size, stereochemical arrangement, and distribution of functional groups.Protein Sorting Signals: Amino acid sequences found in transported proteins that selectively guide the distribution of the proteins to specific cellular compartments.Lactobacillus: A genus of gram-positive, microaerophilic, rod-shaped bacteria occurring widely in nature. Its species are also part of the many normal flora of the mouth, intestinal tract, and vagina of many mammals, including humans. Pathogenicity from this genus is rare.

*  SoftGenetics - Bioke.com : Sharing Knowledge
Products for: DNA, RNA & Genome Analysis. We offer ... for genetic analysis, like Mutation Surveyor Sequence Analysis and Assembly software, NextGENe 2nd...
https://bioke.com/webshop/supplier/SoftGenetics
*  DnaSP 5.10.01 DNA Sequence Polymorphism - DNA Sequence Analysis - Biology Software Net
DnaSP 5.10.01 DNA Sequence Polymorphism - DNA Sequence Analysis - ... Us. Bio-Soft.Net. DNA Sequence Analysis. DnaSP 5.10.01. DnaSP 5.10.01. Size: 4,741K Language: ... dnasp dna sequence polymorphism dna sequence analysis biology software net add favorite contact us chinese version bioinformatics software blog home page search inside about us bio soft net dna sequence analysis dnasp dnasp size k language english directory dna sequence analysis requirements windows date added web site home page ϸ dnasp dna sequence polymorphism is a software package for the analysis of nucleotide polymorphism from aligned dna sequence data dnasp can estimate several measures of dna sequence variation within and between populations in noncoding synonymous or nonsynonymous sites or in various sorts of codon positions as well as linkage disequilibrium recombination gene flow and gene conversion parameters dnasp can als...
http://en.bio-soft.net/dna/dnasp.html
*  .. DNA Sequencing (Molecular Biology) .. 1. Chain Termination, Sanger Method .. 2. Chain Cleavage,
DNA Sequencing Molecular Biology. One of the ... is determining the sequences of bases in specific segments of DNA ... The sequence information can be used to help deduce ... DNA Sequencing Molecular Biology. One of the fundamental methods of molecular biology is determining the sequences of bases in specific segments of DNA. The growing chain is terminated by incorporating a 2′,3′-dideoxynucleoside triphosphate ddNTP that does not support continued DNA synthesis hence the name chain termination. Polymerization of the new strand requires a free 3′-hydroxyl group Fig. As long as dNTPs are incorporated, there is a 3′ hydroxyl group available for continued polymerization of the growing chain Fig. However, dideoxynucleoside triphosphates ddNTPs lack a 3′-hydroxyl group and terminate chain elongation when incorporated into the DNA. Consequently, synthesis, directed by a sample of template DNA that has a unique primer, in the presence of all four deoxy- and one dideoxynucleoside triphosphate yields a populati...
http://what-when-how.com/molecular-biology/dna-sequencing-molecular-biology/
*  GEN News Highlights Related To | GEN
Fingerprints. Analysis of the RNA-Seq and SCA Publication ... Fingerprints. Analysis of the RNA-Seq and SCA Publication ... Market & Tech Analysis. New Protein Clouds IP Landscape for ... Analysis of the RNA-Seq and SCA Publication Landscape RNA-Seq and SCA Could Have a....
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*  Refractory pemphigus vulgaris associated with herpes infection: case report and review
reaction PCR and DNA sequence analysis have been used to ... detect the DNA of HSV 1/2, VZV, EBV, CMV, HHV-6, HHV-7...
http://scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652011000200010&lng=en&nrm=iso
*  Complete sequence of the mitochondrial DNA of Chlamydomonas eugametos | DeepDyve
Complete sequence of the mitochondrial DNA of ... Free. Complete sequence of the mitochondrial DNA of ... complete nucleotide sequence of the Chlamydomonas eugametos...
https://deepdyve.com/lp/springer-journals/complete-sequence-of-the-mitochondrial-dna-of-chlamydomonas-eugametos-WYA8UG80MG/1
*  Education in Bioinformatics
gene structure and sequence, protein structure and function The use ... Introduction to sequence, gene expression, mutation and ... Overview of sequence analysis tools The use of computers in DNA sequencing Introduction to, and ... Courses, Workshops and Seminars in Bioinformatics. Universities link of the International Society of Computational Biology Tutorials and Lecture Notes list of the CCP11 Bioinformatics Courses as listed by Wentian Li. Essential concepts on gene structure and sequence, protein structure and function The use of the internet in biology and medicine Access to biological data with internet services Bibliographic search using Entrez and PubMed Practical exercises with internet services Introduction to sequence, gene expression, mutation and transgenic databases Overview of sequence analysis tools The use of computers in DNA sequencing Introduction to, and practical exercises with, GCG software Algorithms and analysis of sequence comparison data Pattern matching with consensus sequence...
http://csd.hku.hk/bruhk/education.html
*  Cryptococcus albidosimilis
by ribosomal DNA sequence analysis. J Microbiol. 46:7-27 When ... Cryptococcus albidosimilis Cryptococcus albidosimilis. 'Cryptococcus albidosimilis' is a species that has been isolated from soil in Antarctica. Fonseca A., Scorzeti G., and Fell J. 2000 Diversity in the yeast Cryptococcus albidus and related species as revealed by ribosomal DNA sequence analysis. J Microbiol. 46:7-27 When plated on agar it produces colonies that are shining white. The colonies appear to be mucosoid in appearance when plated on agar. When grown in liquid media, the yeast fails to grow well unless the media is constantly agitated. Vishniac H. S., Kurtzman C. P 1992 Cryptococcus anarcticus sp. nov. and Cryptococcus albidosimilis sp. nov., Basidioblasomycetes from Antarctic Soils. Int. J Syst. Bacteriol. 42 4 547-553 This species is considered mesophilic, with optimal growth temperature is at 25°C, with a maximum growth temperature. 2000 Cryptococcus adeliensis sp. nov., a xylanase producing basidiomycetous yeast from Antarctica....
https://en.wikipedia.org/wiki/Cryptococcus_albidosimilis
*  seqapp -- Mac sequence analysis
seqapp -- Mac sequence analysis. seqapp -- Mac sequence analysis Don Gilbert gilbertd at sunflower.bio ... is a Macintosh biosequence editor, analyzer, and network handyman...
http://bio.net/bionet/mm/bio-soft/1993-November/005958.html
*  UniProt: Q36775
-Gene 1. Protein sequence 1. RefSeq pep 1. DNA sequence 2. ... DT 01-NOV-1997, sequence version 1. DT 16-SEP-2015, entry ... ; RN RP NUCLEOTIDE SEQUENCE. RC STRAIN=Norwegian coastal 1; RX...
http://genome.jp/dbget-bin/www_bget?uniprot:Q36775
*  Products - GS FLX+ System : 454 Life Sciences, a Roche Company
... GS FLX+ System. GS FLX+ System. GS FLX+ System Now delivering sequencing reads up to 1,000 bp in length. Available as an on-site instrument upgrade or new instrument, the GS FLX+ System is designed for use with both the new long-read Sequencing Kit XL+ and existing Sequencing Kit XLR70. GS FLX+ System Sequencing Kit. GS FLX Titanium XLR70 Read Length Up to 1,000 bp Up to 600 bp. Mode Read Length 700 bp 450 bp. Throughput Profile - 85% of total bases from reads >500 bp - 45% of total bases from reads >700 bp - 85% of total bases from reads > 300 bp - 20% of total bases from reads > 500 bp. Computing GS FLX+ Computing Station available. monitor 82.5 cm 32.5” H. Lower assembly 75.2 cm × 90.8 cm × 92.7 cm 29.62” × 35.75” × 36.5” W × D × H. GS FLX Titanium Rapid Library MID Adaptors 1 Kit for 96 library preparations 05619211001. GS FLX Titanium Library Paired End Adaptors 1 kit for 10 assays 05463343001. GS FLX Titanium General Library Preparation Kit 1 kit for 10 library preparations 05233747001. GS FLX Tita...
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*  ORNL, Yale Team Advances Virtual Nanopore Method with an Eye to Third-Gen Sequencing System | Genome
ORNL, Yale Team Advances Virtual Nanopore Method with an Eye to Third-Gen Sequencing System. GenomeWeb. Skip to main content. RSS Feeds Twitter LinkedIn. Log in Join now. Business Policy. Business News Research Funding Policy Legislation Regulatory News Reimbursement. Technology. Microarrays Multiplexing PCR Informatics Sequencing Technology Mass Spec Sample Prep Gene Silencing/Gene Editing. Research. Genetic Research Gene Expression Research Epigenetics Research Proteomics Protein Research Cell Biology Research. Clinical. Molecular Diagnostics Companion Diagnostics Biomarker Discovery Validation Drug Discovery Development Clinical Sequencing Clinical Proteomics. Disease Areas. Cancer Infectious Disease Cardiovascular Disease Neurological Psychological Disease Metabolic Disease Autoimmune Disease Inherited Disease Reproductive Health. Applied Markets. Resources. Webinars White Papers The Scan Career Blog Job Listings IP Update New Products People in the News Conferences Events. Main menu. Enter your keywords....
https://genomeweb.com/sequencing/ornl-yale-team-advances-virtual-nanopore-method-eye-third-gen-sequencing-system?utm_source=feedburner&utm_medium=feed&utm_campaign=Feed: genomeweb/insequence (In Sequence)
*  DNA sequencer
These include the 454, SOLiD and Illumina DNA sequencing platforms. 5 More recent, third-generation DNA sequencers such as SMRT and Oxford Nanopore measure the addition of nucleotides to a single DNA molecule in real time. The data may also contain errors, caused by limitations in the DNA sequencing technique or by errors during PCR amplification. Life Technologies. In 2005, 454 Life Sciences released the 454 sequencer, followed by Solexa Genome Analyzer and SOLiD Supported Oligo Ligation Detection by Agencourt in 2006. Applied Biosystems acquired Agencourt in 2006, and in 2007, Roche bought 454 Life Sciences, while Illumina purchased Solexa. More recently, a third generation of DNA sequencers was introduced. Oxford Nanopore Technologies is another company developing third-generation sequencers data using electronic systems based on nanopore sensing technologies. Roche currently manufactures two systems based on their pyrosequencing technology: the GS FLX+ and the GS Junior System. A predecessor to GS FLX+, t...
https://en.wikipedia.org/wiki/DNA_sequencer
*  Genome Sequencing Center offers state-of-the-art sequencing services
... Skip to main navigation University Home MyUCSC People Calendars A-Z Index Search. UC Santa Cruz Menu Newscenter. Awards Honors Events Videos Subscribe Contact Us Helpful Links News by topic News archives E-Newsletter Tuesday Newsday RSS feeds Review Magazine Administrative Messages Budget Updates. Home / 2009 / September / Genome Sequencing Center offers... Genome Sequencing Center offers state-of-the-art sequencing services September 08, 2009 By Tim Stephens, Staff Writer 459-2495. Share this story: Twitter Facebook Google+ LinkedIn Reddit. The UCSC Genome Sequencing Center features state-of-the-art equipment, including the GS FLX Titanium Series sequencing platform from 454 Life Sciences and the SOLiD sequencing platform from Applied Biosystems. Applications include whole-genome and targeted sequencing; resequencing; RNA sequencing; micro-RNA and small-RNA sequencing; chromatin immunoprecipitation ChIP sequencing to identify binding sites of DNA-associated proteins ; and metagenomics also called enviro...
http://news.ucsc.edu/2009/09/3177.html
*  University of Oxford to Sequence Whole Genomes of 500 People Using Illumina Technology | GEN News
University of Oxford to Sequence Whole Genomes of 500 People Using Illumina Technology. Transitioning from Traditional Assay Formats to HTRF Technology. FDA Nominee Driven by "Data, Data, Data". Jobs Report: Data, Business, and Regulatory Savvy Driving New Hiring. Transitioning from Traditional Assay Formats to HTRF Technology. FDA Nominee Driven by "Data, Data, Data". Jobs Report: Data, Business, and Regulatory Savvy Driving New Hiring. Market & Tech An... Transitioning from Traditional Assay Formats to HTRF Technology Sensitivity of Fluorescence Coupled to Low... GEN News Highlights More » Aug 3, 2011 University of Oxford to Sequence Whole Genomes of 500 People Using Illumina Technology Page 1 of 1 Clinicians and geneticists at the University of Oxford have decided to use Illumina s HiSeq 2000 systems to sequence the whole genomes of 500 individuals afflicted with life-threatening diseases. "Our collaboration with Illumina, studying over a hundred different diseases, will allow us to explore the value of wh...
http://genengnews.com/gen-news-highlights/university-of-oxford-to-sequence-whole-genomes-of-500-people-using-illumina-technology/81245503/?kwrd=University of Oxford
*  Unanswered 'dna-sequencing' Questions - Biology Stack Exchange
Unanswered 'dna-sequencing' Questions - Biology Stack Exchange. more stack exchange communities. Stack Exchange. Help Center Detailed answers to any questions you might have. 0 votes. 18 views. microbiology bacteriology dna-sequencing asked yesterday. 0 votes. 27 views. genetics dna-sequencing asked Sep 13 at 11:31. 1 0 votes. 36 views. dna-sequencing virology asked Sep 10 at 21:02. 4 0 votes. 30 views. What do Illumina HiSeq/MiSeq paired end reads look like. dna-sequencing sequence-analysis asked Sep 9 at 17:14. 0 votes. 28 views. Primer Design with Primer-BLAST over specific site I am trying to design primers using Primer-BLAST such that the forward primer spans a specific base pair site. dna-sequencing primer asked Jul 20 at 21:16. 5 votes. 0 answers. 117 views. molecular-genetics dna-sequencing genomics asked Nov 27 '12 at 16:38. 4 votes. 0 answers. 43 views. bioinformatics dna-sequencing sequence-assembly asked Apr 20 at 18:46. 3 3 votes. 0 answers. 27 views. dna dna-sequencing asked Sep 19 at 14...
http://biology.stackexchange.com/questions/tagged/dna-sequencing?sort=unanswered&pagesize=30
*  Re: Log sequence error on db recover
... To : tonye@billy.demon.nl, openldap-software@OpenLDAP.org Subject : Re: Log sequence error on db recover From : Manoj Lal. mlal1974@hotmail.com. Hi Tonni, Yes the db* files are intact after the reboot, but the log files and the bdb files are corrupted. I guess I will have to ensure existence of an ldif to fall back on in case of failure. From: Tony Earnshaw tonye@billy.demon.nl To: openldap-software@OpenLDAP.org Subject: Re: Log sequence error on db recover Date: Sat, 07 Feb 2004 10:46:47 +0100 fre, 06.02.2004 kl. 23.44 skrev Manoj Lal: Try mv'ing the log to an invalid name and running 'db recover -c' again. But bdb files don't get generated. # db recover -c -v db recover: Finding last valid log LSN: file: 4 offset 159583 db recover: Recovery starting from db recover: Recovery complete at Sat Jan 24 07:55:07 2004 db recover: Maximum transaction ID 8000016e Recovery checkpoint db recover: Recovery complete at Sat Jan 24 07:55:07 2004 db recover: Maximum transaction id 80000000 Recovery checkpoint # echo...
http://openldap.org/lists/openldap-software/200402/msg00262.html
*  Nucleotide (Weighted) Links for PubMed (Select 16959970) - Nucleotide - NCBI
Nucleotide Weighted Links for PubMed Select 16959970 - Nucleotide - NCBI. NCBI. DNA RNA. BLAST Basic Local Alignment Search Tool. GenBank. All DNA RNA Resources... BLAST Basic Local Alignment Search Tool. Conserved Domain Search Service CD Search. Search database All Databases Assembly BioProject BioSample BioSystems Books ClinVar Clone Conserved Domains dbGaP dbVar Epigenomics EST Gene Genome GEO DataSets GEO Profiles GSS GTR HomoloGene MedGen MeSH NCBI Web Site NLM Catalog Nucleotide OMIM PMC PopSet Probe Protein Protein Clusters PubChem BioAssay PubChem Compound PubChem Substance PubMed PubMed Health SNP SRA Structure Taxonomy ToolKit ToolKitAll ToolKitBook UniGene Search term. Format Summary GenBank GenBank full FASTA ASN.1 XML INSDSeq XML TinySeq XML Feature Table Accession List GI List. Sort by Default order Accession Date Modified Date Released Organism Name Taxonomy ID. Escherichia coli KRX genome assembly KRX, contig KRX contig52, whole genome shotgun sequence. Escherichia coli KRX genome assembly KR...
http://ncbi.nlm.nih.gov/nuccore?LinkName=pubmed_nuccore_weighted&from_uid=16959970
*  Sequencing technologies - the next generation (With NOTES) - ResearchGate
Nature Reviews Genetics. applications of next-generation sequencing REVIEWS NATuRe RevIewS. Seq-based methods Assays that use next-generation sequencing technologies. next-generation sequencing technologies Sequencing technologies include a number of methods that are grouped broadly as template preparation, sequencing and imaging, and data analysis. There are two methods used in preparing templates for NGS reactions: clonally amplified templates origi- nating from single DNA molecules, and single DNA- molecule templates. Modified nucleotides used in next-generation sequencing methods At the core of most next-generation sequencing NGS methods is the use of dye-labelled modified nucleotides. LI-COR Biosciences c Real-time nucleotides b 3′-unblocked reversible terminators a 3′-blocked reversible terminators HN Illumina/Solexa HN Ju et al. The one-colour images highlight the sequencing data from two single-molecule templates. In the first SBL cycle, the A1 primer was annealed to the template, followed by the hybr...
http://researchgate.net/publication/40483884_Sequencing_technologies_-_the_next_generation_(With_NOTES)
*  .. IDT to Provide Custom Primers for Sequencing on 454’s GS FLX and GS Junior P
. Send to printer. GEN News Highlights : Oct 5, 2010. IDT to Provide Custom Primers for Sequencing on 454’s GS FLX and GS Junior Platforms. Firm offers FusionPrimers and Rapid Libary MID Adaptor Oligos for amplicon or shotgun sequencing. Custom oligos firm Integrated DNA Technologies IDT signed an exclusive deal with the Roche company, 454 Life Sciences, for the provision of custom primers for GS FLX Titanium Chemistry used on the latter s GS FLX and GS Junior systems. The products will be available as FusionPrimers or Rapid Library MID Molecular Identification Adaptor Oligos for amplicon or shotgun sequencing. IDT says the GS FLX Titanium FusionPrimers have a 25 base-pair fixed sequence at the 5 end and are designed to enable sequencing of longer amplicons and increased reads per run. An MID sequence for the FusionPrimers is optional and acts as a molecular barcode to enable libraries to be multiplex and identified. The GS FLX Titanium RapidLibrary MID Adaptor Oligos are deisgned for shotgun sequencing and i...
http://genengnews.com/keywordsandtools/print/4/20676/
*  ARS | Publication request: High Throughput Sequence Analysis for Disease Resistance in Maize ARS : R
Publication request: High Throughput Sequence Analysis for Disease Resistance in Maize ARS : Research. Title: High Throughput Sequence Analysis for Disease Resistance in Maize Authors. High Throughput Sequence Analysis for Disease Resistance in Maize. Raw sequence reads were 26bp and 36bp for maize and Aspergillus, respectively. Most reads contained portions of the Solexa gene expression adapter sequences, which were removed before performing alignment, resulting in an average read length of about 18bp for both maize and Aspergillus data. After filtering, Phred quality was analyzed and determined to be on average 31 for maize and 28 for Aspergillus sequences. Initial mappings on maize produced an average of 98% of sequences mapping to a genomic location with up to two mismatches allowed, with about 57% mapping uniquely within the best stratum. Aspergillus sequences fared better with 91% of sequences mapping and almost 73% mapping to a unique genomic location. An overview of possible further computational appr...
http://ars.usda.gov/research/publications/publications.htm?SEQ_NO_115=251303
*  DNA sequencing
The rapid speed of sequencing attained with modern DNA sequencing technology has been instrumental in the sequencing of complete DNA sequences, or genomes of numerous types and species of life, including the human genome and other complete DNA sequences of many animal, plant, and microbial species. Following the development of fluorescence -based sequencing methods with a DNA sequencer,. Next-generation sequencing methods. Illumina Solexa sequencing. Methods in development Nanopore DNA sequencing. However, there are many other bases that may be present in a molecule. By 2001, shotgun sequencing methods had been used to produce a draft sequence of the human genome. The DNA sample preparation and random surface-PCR arraying methods described in this patent, coupled to Roger Tsien et al.'s "base-by-base" sequencing method, is now implemented in Illumina 's Hi-Seq genome sequencers. The term "'de novo' sequencing" specifically refers to methods used to determine the sequence of DNA with no previously known sequen...
https://en.wikipedia.org/wiki/DNA_sequencing
*  Sequi-Gen GT System | Life Science Education | Bio-Rad
Sequi-Gen GT System. Interchangeable Sequi-Gen GT IPC sizes. Interchangeable Sequi-Gen GT IPC sizes Four Interchangeable Sequi-Gen GT IPC sizes. In addition to DNA sequencing, this system is useful for a wide array of applications: Differential display Microsatellite analysis DNA fingerprinting and footprinting Single-strand conformation polymorphism SSCP studies Heteroduplex analysis RNase protection assays S1 nuclease mapping Primer extension analysis DNA/protein binding studies gel shift assays Oligonucleotide analysis The patented Sequi-Gen GT nucleic acid sequencing cell makes casting and running sequencing gels easy. Simple, problem-free horizontal gel casting Unique design for heat dissipation maintains uniform temperature and eliminates smile patterns Easy-to-use, lever-operated clamps facilitate rapid assembly and gel casting without tape and grease Modular design includes a universal transparent base for all four Sequi-Gen sizes Convenient drain port in the sealed upper buffer chamber allows safe an...
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*  molecular biology - What is the difference between SOLiD, 454, and Illumina next-gen sequencing? - B
molecular biology - What is the difference between SOLiD, 454, and Illumina next-gen sequencing. - Biology Stack Exchange. Biology. Biology Meta. more stack exchange communities. Stack Exchange. Help Center Detailed answers to any questions you might have. Biology Questions. Sign up. Biology Stack Exchange is a question and answer site for biology researchers, academics, and students. What is the difference between SOLiD, 454, and Illumina next-gen sequencing. up vote 9 down vote favorite 3. molecular-biology genomics dna-sequencing share. improve this question. edited Feb 5 '13 at 20:12. asked Feb 5 '13 at 19:55. MattDMo 6,151. GWW Feb 5 '13 at 20:07. MattDMo Feb 5 '13 at 20:09. 2 Answers 2 active oldest votes. The fragments are mixed with agarose beads, which carry adapters complementary to the library adapters, and thus each bead is associated with a unique DNA fragment. Although sequencing on 454 platform is more expensive than sequencing on Illumina platform 40USD per Mega base versus 2USD per Mega base,...
http://biology.stackexchange.com/questions/7075/what-is-the-difference-between-solid-454-and-illumina-next-gen-sequencing
*  Finished sequence of human chromosome 20
finished sequence of human chromosome home about topics finished sequence of human chromosome by edward r winstead december researchers at the wellcome trust sanger institute in cambridge u k have published the dna sequence of human chromosome they identified genes including novel genes the annotation was done through comparative analyses using the protein sets of sequenced organisms partial human gene sequences or ests and mouse and pufferfish genomic sequences the mouse and pufferfish data were not available when a draft of this chromosome was published in february chromosome is about million base pairs long and the new sequence has four gaps or regions that were extremely difficult to analyze the third smallest human chromosome contains genes involved in creutzfeldt jakob disease and severe combined immunodeficiency it is the third finished chromosome published by members of the international consortium known as...
http://genomenewsnetwork.org/articles/12_01/Human_chromosome_20.shtml
*  Next Generation Sequencing (Life Sciences) > Sequencing Instruments and Kits Laboratory Product Dir
Products Reviews. Read more Articles Videos. Latest Editorial Buying Guides Mass Spectrometry. Liquid Handling. Microscopy. DNA and RNA Purification. < Products & Reviews < Life Sciences < Next Generation Sequencing Sequencing Instruments and Kits 48 ChiP Sequencing 22 de novo Sequencing 2 DNA Sequencing 5 Show more...test. Compare or Request Pricing for Selected Product s Chromatrap® Standard Pro-A 96 Well Microplate Sonication Shearing Kit Porvair Sciences Ltd The Chromatrap® high throughput ChIP microplate is based on the novel spin-column ChIP technique It is essentially 96 separate Chromatrap® spin-... Compare or Request Pricing for Selected Product s Enzymatic Chromatrap® Standard Pro-A 96 Well Microplate Shearing Kit Porvair Sciences Ltd The Chromatrap® high throughput ChIP microplate is based on the novel spin-column ChIP technique It is essentially 96 separate Chromatrap® spin-c... Compare or Request Pricing for Selected Product s Chromatrap® Standard Pro G-96 Well Microplate Sonication Shearing Kit ...
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*  Next Generation Sequencing (Life Sciences) > Sequencing Instruments and Kits Laboratory Product Dir
Products Reviews. Read more Articles Videos. Latest Editorial Buying Guides Mass Spectrometry. Microscopy. DNA and RNA Purification. < Products & Reviews < Life Sciences < Next Generation Sequencing Sequencing Instruments and Kits 48 ChiP Sequencing 22 de novo Sequencing 2 DNA Sequencing 5 Show more...test. Compare or Request Pricing for Selected Product s Chromatrap® Standard Pro-A 96 Well Microplate Sonication Shearing Kit Porvair Sciences Ltd The Chromatrap® high throughput ChIP microplate is based on the novel spin-column ChIP technique It is essentially 96 separate Chromatrap® spin-... Compare or Request Pricing for Selected Product s Enzymatic Chromatrap® Standard Pro-A 96 Well Microplate Shearing Kit Porvair Sciences Ltd The Chromatrap® high throughput ChIP microplate is based on the novel spin-column ChIP technique It is essentially 96 separate Chromatrap® spin-c... Compare or Request Pricing for Selected Product s Chromatrap® Standard Pro G-96 Well Microplate Sonication Shearing Kit Porvair Sciences ...
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*  Next Generation Sequencing (Life Sciences) > Sequencing Instruments and Kits Laboratory Product Dir
Products Reviews. Read more Articles Videos. Latest Editorial Buying Guides Mass Spectrometry. Microscopy. DNA and RNA Purification. < Products & Reviews < Life Sciences < Next Generation Sequencing Sequencing Instruments and Kits 48 ChiP Sequencing 22 de novo Sequencing 2 DNA Sequencing 5 Show more...test. Compare or Request Pricing for Selected Product s Chromatrap® Standard Pro-A 96 Well Microplate Sonication Shearing Kit Porvair Sciences Ltd The Chromatrap® high throughput ChIP microplate is based on the novel spin-column ChIP technique It is essentially 96 separate Chromatrap® spin-... Compare or Request Pricing for Selected Product s Enzymatic Chromatrap® Standard Pro-A 96 Well Microplate Shearing Kit Porvair Sciences Ltd The Chromatrap® high throughput ChIP microplate is based on the novel spin-column ChIP technique It is essentially 96 separate Chromatrap® spin-c... Compare or Request Pricing for Selected Product s Chromatrap® Standard Pro G-96 Well Microplate Sonication Shearing Kit Porvair Sciences ...
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*  Next Generation Sequencing (Life Sciences) > Sequencing Instruments and Kits > ChiP Sequencing Labo
Products Reviews. Read more Articles Videos. Latest Editorial Buying Guides Mass Spectrometry. DNA and RNA Purification. < Products & Reviews < Life Sciences < Next Generation Sequencing < Sequencing Instruments and Kits ChiP Sequencing 22. Compare or Request Pricing for Selected Product s Chromatrap® Standard Pro-A 96 Well Microplate Sonication Shearing Kit Porvair Sciences Ltd The Chromatrap® high throughput ChIP microplate is based on the novel spin-column ChIP technique It is essentially 96 separate Chromatrap® spin-... Compare or Request Pricing for Selected Product s Enzymatic Chromatrap® Standard Pro-A 96 Well Microplate Shearing Kit Porvair Sciences Ltd The Chromatrap® high throughput ChIP microplate is based on the novel spin-column ChIP technique It is essentially 96 separate Chromatrap® spin-c... Compare or Request Pricing for Selected Product s Chromatrap® Standard Pro G-96 Well Microplate Sonication Shearing Kit Porvair Sciences Ltd The Chromatrap® high throughput ChIP microplate is based on the ...
http://selectscience.net/next-generation-sequencing-(life-sciences)/product-directory/chip-sequencing/?catID=4741&u=5A4CAC50-53DC-41D4-9F65-8FDA5DFB81D0
*  Next Generation Sequencing (Life Sciences) > Sequencing Instruments and Kits > ChiP Sequencing Labo
Products Reviews. Read more Articles Videos. Latest Editorial Buying Guides Mass Spectrometry. DNA and RNA Purification. < Products & Reviews < Life Sciences < Next Generation Sequencing < Sequencing Instruments and Kits ChiP Sequencing 22. Compare or Request Pricing for Selected Product s Chromatrap® Standard Pro-A 96 Well Microplate Sonication Shearing Kit Porvair Sciences Ltd The Chromatrap® high throughput ChIP microplate is based on the novel spin-column ChIP technique It is essentially 96 separate Chromatrap® spin-... Compare or Request Pricing for Selected Product s Enzymatic Chromatrap® Standard Pro-A 96 Well Microplate Shearing Kit Porvair Sciences Ltd The Chromatrap® high throughput ChIP microplate is based on the novel spin-column ChIP technique It is essentially 96 separate Chromatrap® spin-c... Compare or Request Pricing for Selected Product s Chromatrap® Standard Pro G-96 Well Microplate Sonication Shearing Kit Porvair Sciences Ltd The Chromatrap® high throughput ChIP microplate is based on the ...
http://selectscience.net/next-generation-sequencing-(life-sciences)/product-directory/chip-sequencing/?catID=4741&u=DC157CD8-B624-4748-8B4A-5FE3931221CB
*  Next Generation Sequencing (Life Sciences) > Sequencing Instruments and Kits > ChiP Sequencing Labo
Read more Articles Videos. Latest Editorial Buying Guides Mass Spectrometry. DNA and RNA Purification. < Products & Reviews < Life Sciences < Next Generation Sequencing < Sequencing Instruments and Kits ChiP Sequencing 22. Compare or Request Pricing for Selected Product s Chromatrap® Standard Pro-A 96 Well Microplate Sonication Shearing Kit Porvair Sciences Ltd The Chromatrap® high throughput ChIP microplate is based on the novel spin-column ChIP technique It is essentially 96 separate Chromatrap® spin-... Compare or Request Pricing for Selected Product s Enzymatic Chromatrap® Standard Pro-A 96 Well Microplate Shearing Kit Porvair Sciences Ltd The Chromatrap® high throughput ChIP microplate is based on the novel spin-column ChIP technique It is essentially 96 separate Chromatrap® spin-c... Compare or Request Pricing for Selected Product s Chromatrap® Standard Pro G-96 Well Microplate Sonication Shearing Kit Porvair Sciences Ltd The Chromatrap® high throughput ChIP microplate is based on the novel spin-column ...
http://selectscience.net/next-generation-sequencing-(life-sciences)/product-directory/chip-sequencing/?catID=4741&u=356629B4-AA02-4250-8BCD-DCED93DB8A9D
*  Next Generation Sequencing (Life Sciences) > Sequencing Instruments and Kits > ChiP Sequencing Labo
Read more Articles Videos. Latest Editorial Buying Guides Mass Spectrometry. DNA and RNA Purification. < Products & Reviews < Life Sciences < Next Generation Sequencing < Sequencing Instruments and Kits ChiP Sequencing 22. Compare or Request Pricing for Selected Product s Chromatrap® Standard Pro-A 96 Well Microplate Sonication Shearing Kit Porvair Sciences Ltd The Chromatrap® high throughput ChIP microplate is based on the novel spin-column ChIP technique It is essentially 96 separate Chromatrap® spin-... Compare or Request Pricing for Selected Product s Enzymatic Chromatrap® Standard Pro-A 96 Well Microplate Shearing Kit Porvair Sciences Ltd The Chromatrap® high throughput ChIP microplate is based on the novel spin-column ChIP technique It is essentially 96 separate Chromatrap® spin-c... Compare or Request Pricing for Selected Product s Chromatrap® Standard Pro G-96 Well Microplate Sonication Shearing Kit Porvair Sciences Ltd The Chromatrap® high throughput ChIP microplate is based on the novel spin-column ...
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*  Next Generation Sequencing (Life Sciences) > Sequencing Instruments and Kits > ChiP Sequencing Labo
Read more Articles Videos. Latest Editorial Buying Guides Mass Spectrometry. DNA and RNA Purification. < Products & Reviews < Life Sciences < Next Generation Sequencing < Sequencing Instruments and Kits ChiP Sequencing 22. Compare or Request Pricing for Selected Product s Chromatrap® Standard Pro-A 96 Well Microplate Sonication Shearing Kit Porvair Sciences Ltd The Chromatrap® high throughput ChIP microplate is based on the novel spin-column ChIP technique It is essentially 96 separate Chromatrap® spin-... Compare or Request Pricing for Selected Product s Enzymatic Chromatrap® Standard Pro-A 96 Well Microplate Shearing Kit Porvair Sciences Ltd The Chromatrap® high throughput ChIP microplate is based on the novel spin-column ChIP technique It is essentially 96 separate Chromatrap® spin-c... Compare or Request Pricing for Selected Product s Chromatrap® Standard Pro G-96 Well Microplate Sonication Shearing Kit Porvair Sciences Ltd The Chromatrap® high throughput ChIP microplate is based on the novel spin-column ...
http://selectscience.net/next-generation-sequencing-(life-sciences)/product-directory/chip-sequencing/?catID=4741&u=C91A6BAF-724C-4F1B-B2FB-E534582F609F
*  Next Generation Sequencing (Life Sciences) > Sequencing Instruments and Kits > ChiP Sequencing Labo
Read more Articles Videos. Latest Editorial Buying Guides Mass Spectrometry. DNA and RNA Purification. < Products & Reviews < Life Sciences < Next Generation Sequencing < Sequencing Instruments and Kits ChiP Sequencing 22. Compare or Request Pricing for Selected Product s Chromatrap® Standard Pro-A 96 Well Microplate Sonication Shearing Kit Porvair Sciences Ltd The Chromatrap® high throughput ChIP microplate is based on the novel spin-column ChIP technique It is essentially 96 separate Chromatrap® spin-... Compare or Request Pricing for Selected Product s Enzymatic Chromatrap® Standard Pro-A 96 Well Microplate Shearing Kit Porvair Sciences Ltd The Chromatrap® high throughput ChIP microplate is based on the novel spin-column ChIP technique It is essentially 96 separate Chromatrap® spin-c... Compare or Request Pricing for Selected Product s Chromatrap® Standard Pro G-96 Well Microplate Sonication Shearing Kit Porvair Sciences Ltd The Chromatrap® high throughput ChIP microplate is based on the novel spin-column ...
http://selectscience.net/next-generation-sequencing-(life-sciences)/product-directory/chip-sequencing/?catID=4741&u=CA448E1D-39C4-4A9B-959D-BF8A347807C0
*  Next Generation Sequencing (Life Sciences) > Sequencing Instruments and Kits > ChiP Sequencing Labo
Read more Articles Videos. Latest Editorial Buying Guides Mass Spectrometry. DNA and RNA Purification. < Products & Reviews < Life Sciences < Next Generation Sequencing < Sequencing Instruments and Kits ChiP Sequencing 22. Compare or Request Pricing for Selected Product s Chromatrap® Standard Pro-A 96 Well Microplate Sonication Shearing Kit Porvair Sciences Ltd The Chromatrap® high throughput ChIP microplate is based on the novel spin-column ChIP technique It is essentially 96 separate Chromatrap® spin-... Compare or Request Pricing for Selected Product s Enzymatic Chromatrap® Standard Pro-A 96 Well Microplate Shearing Kit Porvair Sciences Ltd The Chromatrap® high throughput ChIP microplate is based on the novel spin-column ChIP technique It is essentially 96 separate Chromatrap® spin-c... Compare or Request Pricing for Selected Product s Chromatrap® Standard Pro G-96 Well Microplate Sonication Shearing Kit Porvair Sciences Ltd The Chromatrap® high throughput ChIP microplate is based on the novel spin-column ...
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*  Patent US6110683 - Automated DNA Sequencer loading dye which contains a lane tracking aid - Google P
A marker to identify individual lanes on an electrophoresis gel run on an automated DNA sequencer, comprising the loading buffer used in the automated DNA sequencer; a DNA sequencing sample; and a lane-identifying marker DNA fragment labeled with a fluorophore, wherein the fluorophore is detectable by the DNA sequencer. A marker to identify individual lanes on an electrophoresis gel run on an automated DNA sequencer, comprising the loading buffer used in the automated DNA sequencer; a DNA sequencing sample; and a lane-identifying marker DNA fragment labeled with a fluorophore, wherein the fluorophore is detectable by the DNA sequencer, and wherein the length of the DNA fragment is selected such that the labeled DNA fragment will enter the gel to a migration distance slightly longer than the migration distance of the first set of sequencing data from the sequencing reaction, such that inclusion of the marker DNA does not obstruct, interfere, or obscure any of the sequencing data. A marker to identify individua...
http://google.ca/patents/US6110683
*  Why are there N's after Sanger sequencing? - Biology Stack Exchange
Why are there N's after Sanger sequencing. - Biology Stack Exchange. Biology. Biology Meta. more stack exchange communities. Stack Exchange. sign up log in tour. Tour Start here for a quick overview of the site. Help Center Detailed answers to any questions you might have. Biology Questions. Users. Sign up. Biology Stack Exchange is a question and answer site for biology researchers, academics, and students. Why are there N's after Sanger sequencing. After sending a DNA sample for sequencing, the resulting sequence had N's in the beginning and end of the sequence. dna-sequencing share. improve this question. add a comment. 2 Answers 2 active oldest votes. up vote 9 down vote. Typically sanger sequencing will run into a few errors. Sometimes the traces will overlap as below in red and the computer will call N. improve this answer. I'll add on to this answer. A Sanger sequence is really only good for at best 800-900 bp. add a comment. up vote 4 down vote. N bases at end of the sequence simply could ...
http://biology.stackexchange.com/questions/1830/why-are-there-ns-after-sanger-sequencing?answertab=votes
*  Medical Xpress - genome sequencing(... continued page 4)
Medical Xpress - genome sequencing ... continued page 4. Home genome sequencing. News tagged with genome sequencing. sort by:. Date. 6 hours. 12 hours. 1 day. 3 days. all. Rank. Last day. 1 week. 1 month. all. LiveRank. Last day. 1 week. 1 month. all. Popular. Last day. 1 week. 1 month. all. Related topics: dna sequences · genome. Cancer. Researchers confirm whole-genome sequencing can successfully identify cancer-related mutations. UT Southwestern Medical Center cancer researchers have demonstrated that whole-genome sequencing can be used to identify patients' risk for hereditary cancer, which can potentially lead to improvements in cancer prevention, ... Dec 23, 2014 1 0. Genetics. A computer platform can pinpoint the genes behind rare diseases that have eluded diagnosis. A computer program that cross-references disease symptoms with DNA sequencing data can detect the faulty genes responsible for rare disorders with greater accuracy than other methods. Developed by scientists at A*STAR, the ... Dec 17, 2014...
http://medicalxpress.com/tags/genome sequencing/page4.html
*  DNA sanger sequencing - Best Price Segment Microsynth AG -
... DNA/RNA Oligo Synthesis. DNA/RNA Analysis & Sequencing. DNA Oligonucleotides Best Price Segment. DNA Oligos — Volume Orders DNA Oligos — Subscription Orders. Standard Segment. Unmodified DNA Oligos Modified DNA Oligos qPCR Probes. Premium Segment. Unmodified DNA Oligos Modified DNA Oligos qPCR Probes Large-Scale DNA Oligos. RNA Oligonucleotides Best Price Segment. RNA Oligos - Volume Orders siRNA - Volume Orders. Standard Segment. Unmodified RNA Oligos Modified RNA Oligos siRNA. Premium Segment. RNA Oligos Large-Scale RNA Oligos. Technical Information. Sanger Sequencing Best Price Segment. Barcode Economy Run Single Tube Barcode HT Plate Service HT Plate Service - Volume Orders. Standard Segment. Economy Run Single Tube HT Plate Service HT Plate Service - Ready-to-Load. Premium Segment. Technical Information. Technical Information. Hints and Tips. DNA/RNA Analysis DNA/RNA Isolation. Project-based Analysis Services Customized qPCR Assays qPCR Assays for Food Testing. Fragment Length Analysis Human Cell Li...
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*  DNA sanger sequencing - Best Price Segment Microsynth AG -
... DNA/RNA Oligo Synthesis. DNA/RNA Analysis & Sequencing. DNA Oligonucleotides Best Price Segment. DNA Oligos — Volume Orders DNA Oligos — Subscription Orders. Standard Segment. Unmodified DNA Oligos Modified DNA Oligos qPCR Probes. Premium Segment. Unmodified DNA Oligos Modified DNA Oligos qPCR Probes Large-Scale DNA Oligos. RNA Oligonucleotides Best Price Segment. RNA Oligos - Volume Orders siRNA - Volume Orders. Standard Segment. Unmodified RNA Oligos Modified RNA Oligos siRNA. Premium Segment. RNA Oligos Large-Scale RNA Oligos. Technical Information. Sanger Sequencing Best Price Segment. Barcode Economy Run Single Tube Barcode HT Plate Service HT Plate Service - Volume Orders. Standard Segment. Economy Run Single Tube HT Plate Service HT Plate Service - Ready-to-Load. Premium Segment. Technical Information. Technical Information. Hints and Tips. DNA/RNA Analysis DNA/RNA Isolation. Project-based Analysis Services Customized qPCR Assays qPCR Assays for Food Testing. Fragment Length Analysis Human Cell Li...
http://microsynth.ch/de/10174/DNA-Sanger-Sequencing.html
*  TraceTuner download | SourceForge.net
TraceTuner download. SourceForge.net. SourceForge. Browse Enterprise Blog Jobs Deals Help. Log In or Join. Solution Centers Go Parallel Performance Central. Resources Newsletters. Home. Browse. Science Engineering. Bio-Informatics TraceTuner. TraceTuner DNA sequencing quality values, base calling and trace processing Brought to you by: gdenisov. Summary. Files. Reviews. Support. Wiki. Code. Tickets ▾. Feature Requests. Bugs. 5.0 Stars 3. 4 Downloads This Week. Last Update: 2013-12-14. Download tracetuner 3.0.6beta.tar.bz2. Browse All Files. Windows BSD Linux. Screenshots. Description Tracetuner is a tool for base and quality calling of trace files from DNA sequencing instruments. Originally developed by Paracel, a Celera Business, this code base was released as open source in 2006. TraceTuner was used by Celera to call 30+ million reads from both Drosophila and human genome sequencing projects. In 2000, Applied Biosystems bundled TraceTuner with ABI3700 Genome Analyzers and shipped it to the customers of thes...
http://sourceforge.net/projects/tracetuner/?sort=date&source=recommended&stars=1
*  Whitepapers for analytics, lab technology, diagnostics, biotechnology
Real-Time Multiplex PCR of five Different DNA Targets Using the LightCycler® 480 System. Mutation Scanning of the Cytidine Deaminase Gene by High-Resolution Melting Curve Analysis Using the LightCycler® 480 System. Metagenomics Analysis Using the Genome Sequencer FLX System. 05-Sep-2007 The Genome Sequencer FLX System from 454 Life Sciences and Roche Applied Science is a versatile sequencing platform suitable for a wide range of applications, including de novo sequencing and assembly of genomic DNA, transcriptome sequencing, small R more. Real-Time PCR Quantification of Plant miRNAs Using Universal ProbeLibrary Technology. Real-Time PCR Quality Control for Gene Expression. Profiling Using the LightCycler® 480 System 28-Aug-2007 Quantitative real-time PCR qPCR has become the de facto standard for nucleic acid quantification. New Application for the LightCycler® 480 System: Gene Scanning by High-Resolution Melting. 28-Aug-2007 High-resolution melting HRM is a novel, homogeneous, post-PCR method, enabling genomi...
http://analytica-world.com/en/whitepapers/p3/
*  Phred base calling
'Phred base-calling' is a computer program for identifying a base nucleobase sequence from a fluorescence "trace" data generated by an automated DNA sequencer that uses electrophoresis and 4-fluorescent dye method. 1998 : Base-calling of automated sequencer traces using phred. 1998 : Base-calling of automated sequencer traces using phred. PMID 9521922 When originally developed, Phred produced significantly fewer errors in the data sets examined than other methods, averaging 40–50% fewer errors. The electrophoresis run is monitored by a CCD on the DNA sequencer and this produces a time "trace" data or " chromatogram " of the fluorescent "peaks" that passed the CCD point. Examining the fluorescence peaks in the trace data, we can determine the order of individual bases nucleobase in the DNA. It is currently the most widely used base-calling software program by both academic and commercial DNA sequencing laboratories because of its high base calling accuracy. Phred uses a four-phase procedure as outlined by Ewin...
https://en.wikipedia.org/wiki/Phred_base_calling
*  [linux-dvb] Re: High error rates when running Athcool
Re: High error rates when running Athcool. Re: High error rates when running Athcool Uwe Maier umaier at gmx.de. Tue Feb 7 13:55:29 CET 2006. Previous message: Re: High error rates when running Athcool Next message: Re: High error rates when running Athcool Messages sorted by:. For me it indeed makes a difference: 66W vs. 105W and 40W fewer power consumption means fewer 40W to cool - less noise. On higher load more than 3 recordings at the same time I get artefacts lost frames so I switch it off when a recording starts. Regards, uwe Rudo Thomas wrote: Hi. To keep the power consumption of the machine down I use 'Athcool'. This utility tweaks registers on the Northbridge chip so that when the CPU is idle it goes into a 'deep sleep' mode where is uses very little power. This is the output of 'dvbtune -m' on C5 with Athcool running: Signal=14135, Verror=11891, SNR=40349dB, BlockErrors=99, S|L|C|V|SY. Signal=14135, Verror=13445, SNR=39578dB, BlockErrors=191, S|L|C|V|SY. Signal=14135, Verror=12998, SNR=42662dB, Blo...
http://linuxtv.org/pipermail/linux-dvb/2006-February/008106.html
*  ChiRP-Seq
... 'ChiRP-Seq' Chromatin Isolation by RNA purification is a high-throughput sequencing method to discover regions of the genome which are bound by a specific RNA or a by a ribonucleoprotein containing the RNA of interest. Recent studies have shown that a significant proportion of some genomes including mouse and human genomes synthesize RNA that apparently do not code for proteins. Various genomic methods are being developed to map the functional association of these novel RNA to distinct regions of the genome to gain a better understanding of their function. ChiRP-Seq is one of these new methods which uses the massively parallel sequencing capability of 2nd generation sequencers to catalog the binding sites of these novel RNA molecules on a genome. 'Overview of ChiRP-Seq method:' Tens of oligonucleotide probes are designed to be complementary to the RNA of interest. Cells are cross-linked by UV or formalin and nuclei are isolated from these treated cells. Complexes containing biotin-probe + RNA of interest...
https://en.wikipedia.org/wiki/ChiRP-Seq
*  What does "resequence" mean? (NONE)
What does resequence mean. NONE. What does resequence mean. NONE Clemens Suter-Crazzolara c.suter-crazzolara at dkfz-heidelberg.de. Wed Mar 31 04:27:55 EST 1999. Previous message: What does resequence mean. NONE. Next message: Unwanted fluoresence in liposome-mediated transfection Messages sorted by:. Yang Sheng wrote: Thank you. if for some reason the sequencing reaction was satisfactory double bands, smears, no incorporation of label etc. etc. etc. you may want to decide to resequence yout fragment. clemens suter-crazzolara. Previous message: What does resequence mean. NONE. Next message: Unwanted fluoresence in liposome-mediated transfection Messages sorted by:. More information about the Methods mailing list....
http://bio.net/bionet/mm/methods/1999-March/074679.html
*  BioSolutions: DNA Sequencers
... BioSolutions. Online repository of biological information which aims to create a knowledge base for students by the provision of animations and lectures. DNA Sequencers. DNA sequencers have become more important due to large genomics projects and the need to increase productivity. Modern automated DNA sequencing instruments called DNA sequencers are able to sequence as many as 384 fluoresecently labeled samples in a batch run and perform as many as 24 runs a day. These perform only the size separation and peak reading; the actual sequencing reaction s, cleanup and resuspension in a suitable buffer must be performed separately. The magnitude of the fluorescent signal is related to the number of strands of DNA that are in the reaction. If the initial amount of DNA is small, the signals will be weak. However, the properties of PCR allow one to increase the signal by increasing the number of cycles in the PCR program. A simple DNA sequencer will have one or more lasers that emit at a wavelength that is absor...
http://bioisolutions.blogspot.com/2008/03/dna-sequencers.html
*  Whole Genome Sequencing of Wild Rice Reveals the Mechanisms Underlying Oryza Genome Evolution
... . BGI News More. Complete Genomics Announces UK Epilepsy Society s Acquisition of Its Revolocity Supersequencer. BGI has received accreditation from the College of American Pathologists CAP. BGI Poised to Launch Its Desktop Sequencer BGISEQ-500 This October. Complete Genomics Previews Revolocity Sequencing System at European Human Genetics Conference 2015. Academic and Education More. Ancient Alga Knew How to Survive on Land before It Left Water Evolved into First Plant. BGI Presents the First Gene Catalog of Mouse Gut Microbiome. Lanosterol revealed clues for cataract prevention and treatment. Metagenome-wide association study extended to the oral microbiome and uncovered markers for rheumatoid arthritis. Research Collaboration More. Huawei and BGI Sign Joint Development Agreement on Big Data Storage Systems for Genetic Research at HCC2015. BGI Health Forms Partnership with University of Mayor in South America. BGI Health Joins Hand with Star Metropolis Clinical Laboratories to Provide Genetic Testing S...
http://genomics.cn/en/news/show_news?nid=99454
*  View Profile: Phred - Pet Talk
... Today's Dog of the Day. Today's Cat of the Day. Today's Pet of the Day. New Posts. Today's Posts. Today's Posts. Phred. PetTalk members may login in the top right corner or new users may register in order to post. Phred. Find latest posts. Find latest started threads. View Blog Entries. Mini Statistics Join Date 10-19-2000. Last Activity 05-20-2010 02:17 PM. More 3 Friends captain. AmberLee. krazyaboutkatz. Tab Content. Phred's Activity. Visitor Messages. Friends. Post Areas. Tab Content. All Phred Friends Photos. New Activity. 09-07-2015, 07:32 PM krazyaboutkatz replied to a thread Bichon Frise' addition - Please meet the puppy. 2 new pics page 2 in Dog General Congrats!!!:D Welcome to your new loving and caring forever home handsome Frankie.: I must've missed your latest cat addition because I only see 7... 31 replies. 1242 view s. 09-07-2015, 07:21 PM krazyaboutkatz replied to a thread Sky Is Gone in Cat Memorial Thanks everyone. 26 replies. 788 view s. 09-06-2015, 12:05 AM krazyaboutkatz started a th...
http://petoftheday.com/talk/member.php?714-Phred&tab=activitystream&type=friends
*  Taking Sequencing Forward - Wellcome Trust Sanger Institute
... Jump to navigation. Jump to content. Search for. A A A A. Home. Research. Scientific resources. Work study. About us. What we do. History. How we work. People. Press. Public engagement. Campus. Contact 14th January 2008 Taking Sequencing Forward New Team to Lead DNA Sequencing at the Sanger Institute Sequencing is of fundamental importance to the science of the Sanger Institute now and in the future. The new team will enhance the Institute's global role in delivery of valuable sequence data and analysis and ensure that those efforts meet the needs of the wider community. The Wellcome Trust Sanger Institute has recently made a major investment in new DNA sequencing technology which will increase production 60-fold. The Institute today announces the appointment of a new senior management team to lead the Institute's efforts to generate DNA sequence data of value to its core programmes and to the wider biomedical research community. Professor Julian Parkhill takes on a new role as Director of Sequencing...
http://sanger.ac.uk/about/press/2008/080114.html
*  IntelligentBioSystems.com
intelligentbiosystems com about ibs technology applications career center contact us fail the browser should render some flash content not this the new concept of benchtop sequencing ibs has developed a new type of benchtop sequencing system that is capable of performing up to independent sequencing runs in parallel and allows random loading of the flow cells at any time during a run the new system is just about to enter early beta testing the ibs team intelligent bio systems is a group of innovative and experienced chemists molecular biologists and engineers capable of bringing breakthrough technologies to market quickly click here for a list of open positions at the company about us intelligent bio systems was founded in by drs steven j gordon and jingyue ju to commercialize an advanced dna sequencing system dr gordon previously founded intelligent automation systems which was responsible for a number of key instrumentation solutions which helped to complete the human genome project dr ju is one of the key ...
http://intelligentbiosystems.com/
*  DNASTAR - Training Videos Flash Version | DNASTAR
DNASTAR - Training Videos Flash Version. DNASTAR Lasergene. Molecular Biology. Next-Gen Sequencing. Structural Biology. DNASTAR Cloud. WORKFLOWS. Molecular Biology. Cloning. Gene Discovery. Primer Design. Sanger Sequence Assembly. Sequence Alignment. Structural Biology. Epitope Prediction. Protein Sequence & Structure. Protein Structure Prediction. Structural Alignment. Next-Gen Sequencing. Automated Genome Closure. Cancer Genomics. ChIP-Seq Analysis. De Novo Genome Assembly. De Novo Transcriptome Assembly. Exome Alignment. Gene Panels. Reference Guided Genome Alignment. RNA-Seq Alignment. SNP Validation Control. Variant Analysis. Clinical Research. Association Studies. Cancer Genomics. Gene Expression Patterns. Gene Panels. SUPPORT. Help. Online Help. Request Support. Installation Guides. Licensing Options. Product Literature. Product Notifications. Videos. Webinars. Genome Template Packages. Archived Genome Templates. Supported Sequencing Technologies. dbNSFP Gene Level Annotations. Genome Assembly. Structu...
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*  JGI GOLD | Project
JGI GOLD. Project. JGI HOME LOG IN. Home. Search. Quick Search. Advanced Search. Metadata Search. Distribution Graphs. Biogeographical Metadata. Google Map. Google Earth. Statistics. References. Team. Help. News. Studies. 22,233. Biosamples. 69,152. Sequencing Projects. 69,468. Analysis Projects. 57,279. Project Information. Sequencing Information. Organism Information. Organism Metadata. PROJECT NAME GOLD Project ID. Gp0001259. Project Name. Hoeflea phototrophica DFL-43. Other Names. Legacy ER Project ID. 11154. Legacy GOLD ID. Gi01415. NCBI BioProject Name Hoeflea phototrophica DFL-43. NCBI BioProject ID. 19311. NCBI BioProject Accession. PRJNA19311. NCBI Locus Tag. HPDFL. NCBI BioSample Accession. PI. Added By Nikos Kyrpides on 2007-11-27. Last Modified By Auto script update processes on 2014-10-31. Project Comments. PROJECT TYPE Specimen. Organism. Nucleic Acid. DNA. Sequencing Strategy. Whole Genome Sequencing. PROJECT INFORMATION Project Status. Permanent Draft. Project Relevance. Environmental Marine M...
https://gold.jgi.doe.gov/project?id=Gp0001259
*  Newest 'bioinformatics' Questions - Page 2 - Biology Stack Exchange
Newest 'bioinformatics' Questions - Page 2 - Biology Stack Exchange. Stack Exchange. Help Center Detailed answers to any questions you might have. Biology Questions. Tagged Questions info newest frequent votes active unanswered. 3 votes. 1 answer. 31 views. 0 votes. 0 answers. 51 views. 4 1 vote. 1 answer. 64 views. bioinformatics dna-sequencing pcr sequence-analysis primer asked Jul 1 at 13:56. 3 votes. 2 answers. 56 views. 1 vote. 2 answers. 73 views. bioinformatics asked Jun 24 at 2:26. 7 -1 votes. 1 answer. 51 views. -1 votes. 3 answers. 47 views. bioinformatics proteins dna-sequencing asked Jun 21 at 7:16. 2 1 vote. 1 answer. 196 views. bioinformatics asked Jun 20 at 12:05. genetics neuroscience bioinformatics book-recommendation asked Jun 16 at 15:11. evolution bioinformatics phylogenetics sequence-analysis asked Jun 10 at 12:45. genetics bioinformatics gene-regulation computational-model asked Jun 6 at 21:38. molecular-biology bioinformatics pcr primer asked Jun 4 at 5:45. bioinformatics molecu...
http://biology.stackexchange.com/questions/tagged/bioinformatics?page=2&sort=newest&pagesize=30
*  Genotyping and Gene Expression - The Online Scientific Community - News
... Scientific Community. Predictive Biosciences Announces Next-Generation Sequencing Assay for p53. Predictive Biosciences announced it has developed a new next-generation sequencing NGS assay for the TP53 gene encoding the p53 protein that will enhance the clinical performance of its bladder cancer assay. The new CertNDx TM Bladder Cancer Assay incorporating TP53 is expected to be commercially available in early 2013. Predictive's next-generation sequencing assay for TP53 can detect 133 different mutations previously identified in bladder cancers. A research study conducted on 200 control samples and 36 cancers, incorporating TP53 sequence analysis into the existing assay, demonstrated an improvement over the previously published test by increasing sensitivity across bladder cancer pathways while retaining very high specificity and high Positive Predictive Value PPV. The CertNDx test now fully incorporates the different pathways of bladder cancer. "In addition to incorporating p53 into the existing CertNDx...
http://technologynetworks.com/Genotyping/news.aspx?id=146947
*  .. Whole Genome Sequencing in Diagnostics? .. Share this: .. Like this:
whole genome sequencing in diagnostics by dr bertalan meskã on september using whole genome sequencing in diagnostics has been an issue for years and as the cost of sequencing is rapidly declining it seems it can pave the way for personalized medicine a new research published in genome biology evolution of an adenocarcinoma in response to selection by targeted kinase inhibitors just proves this point adenocarcinomas of the tongue are rare and represent the minority to of salivary gland tumors affecting the tongue we investigated the utility of massively parallel sequencing to characterize an adenocarcinoma of the tongue before and after treatment we conclude that complete genomic characterization of a rare tumor has the potential to aid in clinical decision making and identifying therapeutic approaches where no established treatment protocols exist these results also provide direct in vivo genomic evidence for mutational evolution within a tumor under drug selection and potential mechanisms of drug resistance...
http://scienceroll.com/2010/09/24/whole-genome-sequencing-in-diagnostics/?like=1&_wpnonce=469fbe03a0
*  .. Whole Genome Sequencing in Diagnostics? .. Share this: .. Like this:
Whole Genome Sequencing in Diagnostics. by Dr. Bertalan Meskó on September 24, 2010. Using whole genome sequencing in diagnostics has been an issue for years, and as the cost of sequencing is rapidly declining, it seems it can pave the way for personalized medicine. A new research published in Genome Biology, Evolution of an adenocarcinoma in response to selection by targeted kinase inhibitors, just proves this point:. Adenocarcinomas of the tongue are rare and represent the minority 20 to 25% of salivary gland tumors affecting the tongue. We investigated the utility of massively parallel sequencing to characterize an adenocarcinoma of the tongue, before and after treatment. We conclude that complete genomic characterization of a rare tumor has the potential to aid in clinical decision making and identifying therapeutic approaches where no established treatment protocols exist. These results also provide direct in vivo genomic evidence for mutational evolution within a tumor under drug selection and potentia...
http://scienceroll.com/2010/09/24/whole-genome-sequencing-in-diagnostics/?like=1&_wpnonce=cb13afa090
*  Recent Articles | Whole Genome Sequencing And Neuroscience | The Scientist Magazine®| Page 6
Recent Articles. Whole Genome Sequencing And Neuroscience. The Scientist Magazine. Page 6. The Scientist Sign In or Register. Advertisement. The Scientist. tags: whole genome sequencing x. neuroscience x. The Scientist whole genome sequencing and neuroscience. Most Recent Three Monkey Brains, One Robotic Arm By Jef Akst. July 10, 2015. Researchers network the brains of three monkeys to create a living computer that can steer an image of a robotic arm toward a target. 0 Comments. Gene Therapy Fixes Mouse Hearing By Kerry Grens. July 9, 2015. Expressing a gene for a component of the inner ear s hair cells treated a form of genetic deafness. 0 Comments. Can We Smell A Trillion Odors. By Kerry Grens. July 8, 2015. A reanalysis calls into question a year-old claim that humans can decipher at least 1 trillion different scents. 2 Comments. Capsule Reviews By Bob Grant. July 1, 2015. Stoned, Anxious, The Deeper Genome, and Testosterone 0 Comments. New Human Brain Language Map By Bob Grant. June 26, 2015. Researchers ...
http://the-scientist.com/?articles.list/tagNo/2416,11/tags/whole-genome-sequencing,neuroscience/pageNo/6/
*  King’s Proclamation for Citizen Scientists - Bio-IT World
... News. RELATED STORIES A Worthy Sequel: PacBio's New Sequencing System. King’s Proclamation for Citizen Scientists. We very much hope that something we do in the next 20 years will preclude another million women dying of the disease.”. Back in It was 1994, King, for years the personification of the war against breast cancer, had suffered the scientific equivalent of a death in the family. King had been studying the genetics of breast cancer for two decades, stubbornly believing that the disease must have a hereditary component when most so-called experts rubbished the idea. “BRCA” stands for breast cancer although at the time King joked it signified “Berkeley, California.” The breakthrough had come when King’s group sorted their families by age-of-onset. Seven affected families with early-onset disease provided unequivocal data supporting linkage of a DNA marker on chromosome 17 to breast cancer susceptibility; the signal tailed off in other families, indicating the confounding role of other genetic a...
http://bio-itworld.com/BioIT_Article.aspx?id=120390
*  Project: 2/5-Elucidating the genetic architecture of autism by deep genomic sequencing
... Projects Home Project Detail. 2/5-Elucidating the genetic architecture of autism by deep genomic sequencing. This research will be conducted through a partnership between expert large-scale sequencing centers and a collaborative network of research labs focused on the genetics of autism to utilize novel high-throughput genome sequencing to discover specific genes underlying the significant heritability of autism. Without knowledge of the specific genes and DNA variants that predispose to disease, the basic starting point with which the biological processes of disease might be understood is missing. The project will first support a detailed examination of 1000 genes implicated by previous genetic studies or postulated to be functionally relevant, and later, as the technology continues to advance, through unbiased whole-genome sequencing. By combining large, well-characterized patient samples, experienced DNA sequencing teams and a collaborative, expert analysis and follow-up network, this study will provi...
https://iacc.hhs.gov/apps/portfolio-analysis-web-tool/project?projectId=3665&fy=2010
*  TraceTuner
Files from TraceTuner Tracetuner is a tool for base and quality calling of trace files from DNA sequencing instruments. Originally developed by Paracel, a Celera Business, this code base was released as open source in 2006. TraceTuner was used by Celera to call 30+ million reads from both Drosophila and human genome sequencing projects. In 2000, Applied Biosystems bundled TraceTuner with ABI3700 Genome Analyzers and shipped it to the customers of these capillary electrophoresis sequencers. Later versions of TraceTuner, which support mixed base calling, have been used by the research community, the private biotech sector, and the U.S. government as components of different variant detection, genotyping and forensic software applications (e.g. Applied Biosystems SeqScape, Paracel Genome Assembler, MTexpert, etc.)....
http://sourceforge.net/projects/tracetuner/rss?path=/
*  Sequencing from PCR - Biology Stack Exchange
... Biology. Biology Meta. more stack exchange communities. Stack Exchange. Help Center Detailed answers to any questions you might have. Biology Questions. Biology Stack Exchange is a question and answer site for biology researchers, academics, and students. Sequencing from PCR. My question is, is it possible to sequence DNA after PCR and is it easier than sequencing it via other methods. dna-sequencing pcr share. improve this question. add a comment. 2 Answers 2 active oldest votes. All sequencing methods, be it classical Sanger sequencing or next-generation sequencing or even third generation need a certain amount of DNA to work with. So PCR is used routinely to amplify DNA before sequencing. The sequencing itself isn’t influenced by this – you use whatever technique is appropriate. However, the PCR may introduce biases into the data because not all DNA fragments amplify equally efficiently. Coming back to your question, PCR isn’t a sequencing method in itself. Furthermore, most sequencing methods actuall...
http://biology.stackexchange.com/questions/2804/sequencing-from-pcr/2805
*  bioinformatics - What's the use of DNA sequencing results? - Biology Stack Exchange
- Biology Stack Exchange. Biology Meta. more stack exchange communities. Stack Exchange. sign up log in tour. Help Center Detailed answers to any questions you might have. Biology Questions. Sign up. Biology Stack Exchange is a question and answer site for biology researchers, academics, and students. What's the use of DNA sequencing results. dna bioinformatics dna-sequencing share. add a comment. 3 Answers 3 active oldest votes. up vote 7 down vote. The elephant in the room are of course coding sequences : stretches of DNA, contained in so-called “ genes ” – which code for proteins and other stuff. Once you have found the genes in a sequence which you can trivially translate to amino acid sequences, you can try to form inferences about the function of the proteins a protein’s function is an almost-direct consequence of its sequence. Unfortunately, discovering the function of a protein from its sequence alone isn’t possible but we do know the functions of many proteins. When we now look at a genome and fi...
http://biology.stackexchange.com/questions/1884/whats-the-use-of-dna-sequencing-results/1886
*  Nuclear run-on
About 10 6 Cell nuclei are isolated and incubated with labeled nucleotide s and genes in the process of being transcribed are detected by hybridization of extracted RNA to gene specific probes on a blot. Alternative microarray methods have recently been developed, mainly PolII RIP-chip: RNA immunoprecipitation of RNA polymerase II with phosphorylated C-terminal domain directed antibodies and hybridization on a microarray slide or chip the word chip in the name stems from "ChIP-chip" where a special affymetrix genechip was required. A comparison of methods based on run-on and ChIP-chip has been made in yeast Pelechano et al., 2009. It has to be considered that run-on only detects elongating RNA polymerases whereas ChIP-chip detects all present RNA polymerases, including backtracked ones. thumb|Overview of the Global run on sequencing assay for delineating genes, genome-wide, that are engaged in transcription. Therefore only genes that already have an RNA polymerase will produce labeled transcripts. RNA transcr...
https://en.wikipedia.org/wiki/Nuclear_run-on
*  Sequencing from PCR - Biology Stack Exchange
... Biology. Biology Meta. more stack exchange communities. Stack Exchange. sign up log in tour. Help Center Detailed answers to any questions you might have. Biology Questions. Biology Stack Exchange is a question and answer site for biology researchers, academics, and students. Sequencing from PCR. My question is, is it possible to sequence DNA after PCR and is it easier than sequencing it via other methods. dna-sequencing pcr share. improve this question. add a comment. 2 Answers 2 active oldest votes. All sequencing methods, be it classical Sanger sequencing or next-generation sequencing or even third generation need a certain amount of DNA to work with. You either need to extract DNA from a large-ish tissue sample or you need to amplify DNA content from a smaller sample. So PCR is used routinely to amplify DNA before sequencing. Furthermore, most sequencing methods actually include PCR steps as well, since they rely on the generation of of overlapping DNA fragments to be stitched together. add a comment...
http://biology.stackexchange.com/questions/2804/sequencing-from-pcr?answertab=votes
*  dna sequencing - ChIP-seq vs ChIP-exo - Biology Stack Exchange
... chat blog. Biology. Biology Meta. more stack exchange communities. Stack Exchange. sign up log in tour. help. Help Center Detailed answers to any questions you might have. Biology Questions. Ask Question. Sign up. Biology Stack Exchange is a question and answer site for biology researchers, academics, and students. ChIP-seq vs ChIP-exo. I'm currently investigating ChIP-seq vs. ChIP-exo for finding binding sites. As far as I can tell, ChIP-exo seems to be better in every way than ChIP-seq... Are there any weaknesses in the ChIP-exo approach. dna-sequencing transcription protein-binding binding-sites chip share. improve this question. asked Jun 6 '12 at 3:07. add a comment. 1 Answer 1 active oldest votes. up vote 4 down vote. ChIP-exo does seem to be the "ChIP-seq killer." I've seen Dr. improve this answer. answered Jun 7 '12 at 19:38. add a comment. Your Answer. Email. Email. Not the answer you're looking for. Browse other questions tagged dna-sequencing transcription protein-binding binding-sites chi...
http://biology.stackexchange.com/questions/2512/chip-seq-vs-chip-exo?answertab=active
*  Sequencing from PCR - Biology Stack Exchange
... Biology. Biology Meta. more stack exchange communities. Stack Exchange. sign up log in tour. Help Center Detailed answers to any questions you might have. Biology Questions. Sign up. Biology Stack Exchange is a question and answer site for biology researchers, academics, and students. Sequencing from PCR. up vote 4 down vote favorite. My question is, is it possible to sequence DNA after PCR and is it easier than sequencing it via other methods. dna-sequencing pcr share. improve this question. asked Jul 2 '12 at 0:57. add a comment. 2 Answers 2 active oldest votes. All sequencing methods, be it classical Sanger sequencing or next-generation sequencing or even third generation need a certain amount of DNA to work with. So PCR is used routinely to amplify DNA before sequencing. Furthermore, most sequencing methods actually include PCR steps as well, since they rely on the generation of of overlapping DNA fragments to be stitched together. add a comment. up vote 4 down vote. Yes, I'm actually more intere...
http://biology.stackexchange.com/questions/2804/sequencing-from-pcr
*  GEN News Highlights Related To | GEN
Linking Phenotypes and Modes of Action Through High-Content Screen Fingerprints. Transitioning from Traditional Assay Formats to HTRF Technology. Linking Phenotypes and Modes of Action Through High-Content Screen Fingerprints. Transitioning from Traditional Assay Formats to HTRF Technology. Linking Phenotypes and Modes of Action Through High-Content Screen Fingerprints The Use of High-Content Screening as... GEN News Highlights Related to Covaris to Supply Sample-Prep Tool for BGI's Next-Generation Sequencing Projects Human Longevity, Discovery to Offer Clients Sequencing, Analysis for $250 NIH Awards $13M in Grants Toward Identifying Genomic Variants Ye Shall Know Them by Their Microbial Clouds Sequencing Metastatic Cancers Could Lead to Improved Therapies Using Nanopores to Study Real-Time Enzymatic Processes Detecting All Infective Viruses at Once Gene Region That Protects Children from Severe Malaria Identified 1000 Genomes Project Wraps Up at 2,504 ZyGEM Bolsters DNA Extraction Kit Distribution in Asia P...
http://genengnews.com/more/related/gen-news-highlights/4/23660/?page=2
*  [Numpy-discussion] Find contiguous sequences of booleans in arrays
find contiguous sequences of booleans in arrays find contiguous sequences of booleans in arrays charles r harris charlesr harris gmail mon sep cdt previous message segfault on wrong use of indexing next message confusion about min max messages sorted by hi kurdt on kurdt bane kurdt bane gmail com wrote hi to all i ve got an d array of bools and i d like to find the length of the first contiguous sequence of true values starting from position of the array that s equivalent to find the position of the first occurrence of false in the array the problem is trivial but i was wondering what s the best fastest cleanest most pythonesque way to do it in numpy and what if i want to get a list of all the contiguous sequences of true values above a given threshold you can find the start of all runs after the first by in a array dtype bool in s arange len a a in s out array i e the first run is a the second a etc and the runs alternate between true and false chuck next part an html attachment was scrubbed url http project...
https://mail.scipy.org/pipermail/numpy-discussion/2007-September/029250.html
*  SHOTGUN SEQUENCING
... Bruce Roe broe at aardvark.ucs.uoknor.edu. Mon Dec 13 08:30:00 EST 1993. Previous message: SHOTGUN SEQUENCING Next message: Rainbow markers Messages sorted by:. In article 1993Dec7.084144.4413 at crc.ac.uk, mjones at crc.ac.uk Dr. M.D. Jones writes... Hi netters One of the favoured ways of sequencing large DNA fragments is to shotgun clone 'sonicated' DNA into a M13 vector and sequence the clones, and compile the data by computer. In the past I have successfully used this strategy, but the most frustrating steps were getting enough recombinant clones to complete the sequence. This is due to the damage caused by sonicating the DNA, making most of it unclonable. Does anybody out there have a detailed fool-proof 'sonication' protocol. Anybody have any advice/tips/etc on how to get the best out of this procedure. On a similar note, does anybody use 'partial' restriction enzyme digestion to fragment their target DNA. Fitzgerald et al, 1992 Nucleic Acids Res., 20 14, 3753-3762 used the enzyme CviJI recognition...
http://bio.net/bionet/mm/methods/1993-December/009822.html
*  Technique of DNA-sequencing
technique of dna sequencing technique of dna sequencing patrick harenberg ebola at nikocity de fri mar est previous message human genome next message pre registration instructions messages sorted by hi can anyone of you tell me or even send me a paper that exactly describes the technique pcr electrophoresis etc of sequencing dna i e getting the exact base sequence of a viral genome answers please to ebola at nikocity de thanks in advance cu patrick harenberg previous message human genome next message pre registration instructions messages sorted by more information about the biochrom mailing list...
http://bio.net/bionet/mm/biochrom/2002-March/002670.html
*  Genetic Sequencing May Not Be Ready To Become Routine | WUKY
Genetic Sequencing May Not Be Ready To Become Routine. WUKY. Local News Sources. WUKY HD-1. WUKY HD-2. WUKY HD-3. Gallery Hop. KY Theatre Classic Movie Series. WUKY presents... tadoo Lounge. About WUKY. Gallery. Support Us. Support WUKY Through Amazon. Local News Sources. WUKY HD-1. WUKY HD-2. WUKY HD-3. Originally published on March 11, 2014 6:51 pm Transcript ROBERT SIEGEL, HOST: This is ALL THINGS CONSIDERED from NPR News. Getting your entire genetic code deciphered is the cutting edge of medicine. NPR's Rob Stein joins us now to talk about one of the first carefully done studies aimed at answering that question. And, Rob, why don't you back up first and explain just what it means to decipher somebody's entire genetic code. BLOCK: And that was the idea behind this study that I mentioned, trying to figure out the reality here. It's only covered in very specific situations where somebody is using it for a specific medical purpose, like for somebody who has a mysterious condition that nobody can diagnose, or ...
http://wuky.org/post/genetic-sequencing-may-not-be-ready-become-routine
*  ExoSAP-IT reagent: one step to accurate sequencing results | AffymetrixCatalogNav
ExoSAP-IT reagent: one step to accurate sequencing results. Affymetrix. Products. Products. USB Molecular Biology Reagents. Community. Affymetrix Microarray Solutions. About Affymetrix. Contact Us. Products Microarray Solutions Microarray Solutions View all DNA Analysis Solutions Genome-Wide Genotyping for Human Disease Research. RNA Analysis Solutions 3' IVT Expression Analysis. PCR PCR View all End Point PCR DNA Polymerases. PCR Purification. PCR Clean Up View all products >. RT-PCR DNA Polymerases. PCR Purification. Molecular Biology Enzymes Molecular Biology Enzymes View all Binding Proteins. DNA Polymerases. Molecular Biology Kits and Reagents Molecular Biology Kits and Reagents View all Cellular Manipulation View all products >. Home > Promotions > ExoSAP-IT reagent: one step to accurate sequencing results. 100% sample recovery with one-tube, one-step PCR clean-up - no loss of PCR products, regardless of the fragment size. ExoSAP-IT reagent is designed for simple, quick PCR clean-up for downstream a...
http://affymetrix.com/estore/browse/promotionLandingPage.jsp?promotionId=promo610016&navModeValue=35689&isHtmlStatic=true&aIdValue=usbNav&cmpid=USBGENseq128
*  [Bioperl-guts-l] [Bug 2630] Bio::LocatableSeq range validation does not work with translated sequen
Bio::LocatableSeq range validation does not work with translated sequence coordinates. Bio::LocatableSeq range validation does not work with translated sequence coordinates bugzilla-daemon at portal.open-bio.org bugzilla-daemon at portal.open-bio.org. Sun Nov 16 01:06:13 EST 2008. Previous message: seq_inds overhaul Next message: Bio::LocatableSeq range validation does not work with translated sequence coordinates Messages sorted by:. http://bugzilla.open-bio.org/show_bug.cgi?id=2630 ------- Comment #2 from cjfields at bioperl.org 2008-11-16 01:06 EST ------- In reply to comment #1 Initial mapping implementation added to subversion, which takes a two element array or array ref with a parameter and returns a two element array. The two elements are integers not typechecked yet which indicate: 1 # of residues which map to... 2 # of positions based on the LocatableSeq start/end, or the calculated length of the sequence - gaps A translated sequence mapping to nucleotide coordinates would be , while a reverse-trans...
http://bioperl.org/pipermail/bioperl-guts-l/2008-November/027934.html
*  sequencer
... jxli sonytoad at hotmail com sat sep est previous message sequencer next message pooling run folders messages sorted by i think is absolutely better due to abi technology we have a abi and had ever tested a ceq for months we got some good sequence results from ceq as well as our abi and megabace the big problem of ceq is that its laser and fluorescence is very different so you can use reagent only from beckman abi is the update version of abi you know we use many different reagent on abi abi or and these reagent are come from different company such as abi bigdye or amersham et the only excuse to choose ceq is its price if you need more throughput megabace is another choice lijx beijing genome institute www genomics org cn qimin chao qchao at morphotek com wrote in message news n dc fq at mercury hgmp mrc ac uk hi all we are purchasing a new low end capillary sequencing machine and have the choice of abi and beckman ceq anyone has experience with both machines and what are the pros and cons qimin chao ph ...
http://bio.net/bionet/mm/autoseq/2001-September/002301.html
*  Gene density
... in genetics the gene density of an organism s genome is the ratio of the number of genes per number of base pairs usually written in terms of a million base pairs or megabase mb the human genome has a gene density of genes mb while the genome of the c elegans roundworm is estimated to have seemingly simple organisms such as bacteria and amoebas have a much higher gene density than humans bacterial dna has a gene density on the order of genes mb this is due several factors including that the fact that bacterial dna has no introns there are also fewer codon s in bacterial genes see also c value enigma references category genetics category genes...
https://en.wikipedia.org/wiki/Gene_density
*  www.genomebiology.com - Figure 4
www genomebiology com figure resolution standard high figure read coverage depth for models overlapping cdna loci and models not overlapping cdnas the distribution of the average depth log on all exonic nucleotides of the models is plotted for models overlapping cdnas on of their nucleotides green and models not overlapping cdnas black the y axis corresponds to the percentage of models in each bin bin width is denoeud et al genome biology r doi gb r download authors original image...
http://genomebiology.com/2008/9/12/R175/figure/F4
*  DNA SEQUENCERS FOR SALE
http://bio.net/bionet/mm/chlamy/1997-July/000886.html
*  Panasonic 14mm F2.5 review data at various sites.: Micro Four Thirds Talk Forum: Digital Photography
... Review. Reviews. Sample Images. Videos. Lenses. Forums. Forum index. Micro Four Thirds Talk Change forum Panasonic 14mm F2.5 review data at various sites. Shop cameras lenses ▾. Forum Parent First Previous Next Next unread. jericho77. Panasonic 14mm F2.5 review data at various sites. Aug 5, 2013. Reply to thread Reply with quote. Forum Parent First Previous Next Next unread. Posted by When Panasonic 14mm F2.5 review data at various sites. jericho77. Aug 5, 2013. Re: Panasonic 14mm F2.5 review data at various sites. Aug 5, 2013 3. Re: Panasonic 14mm F2.5 review data at various sites. Aug 5, 2013. Re: Panasonic 14mm F2.5 review data at various sites. Aug 5, 2013. Re: Panasonic 14mm F2.5 review data at various sites. jericho77. Aug 5, 2013. Aug 5, 2013. jericho77. Aug 5, 2013. Re: Panasonic 14mm F2.5 review data at various sites. jericho77. Aug 5, 2013. Re: Panasonic 14mm F2.5 review data at various sites. Aug 5, 2013 1. Re: Panasonic 14mm F2.5 review data at various sites. Alumna Gorp. Aug 5, 2013. ...
http://dpreview.com/forums/post/51930238
*  The genetic basis of a plant-insect coevolutionary key innovation. Proc Natl Acad Sci USA (PDF Downl
Proc Natl Acad Sci USA PDF Download Available. Proc Natl Acad Sci USA. © 2007 by The National Academy of Sciences of the USA www.pnas.org?cgi?doi?10.1073?pnas.0706229104PNAS. fossil calibrated, Bayesian relaxed molecular clock estimation of the Pierinae–Coliadinae divergence. Fossil and molecular data agree that the Brassicales appeared by 90 to 85 Mya, which is much earlier than the parallel evolution of glucosinolates in the Euphorbiaceae 58 Mya 28. In this article, we directly address the timing of the appearance of the glucosinolate-feeding Pierinae, using several independent molecular datasets and various calibration methods to generate a robust estimate of when Pieridae evolved relative to their Brassicales host plants. Pieridae-specific temporal reconstruction used a Bayesian relaxed molecular clock method on EF-1. Current and expected diversity, and divergence estimates for the Coliadinae and Pierinae subfamilies of Pieridae. However, there are significantly more species of Pierinae than expected, eve...
http://researchgate.net/publication/5771953_The_genetic_basis_of_a_plant-insect_coevolutionary_key_innovation._Proc_Natl_Acad_Sci_USA
*  Mystery of bacterial growth and resistance solved: Findings shed light on how bacteria form protecti
... ve biofilms -- ScienceDaily. Your source for the latest research news. Mystery of bacterial growth and resistance solved: Findings shed light on how bacteria form protective biofilms. Date: April 26, 2012 Source: The Scripps Research Institute Summary: Scientists have unraveled a complex chemical pathway that enables bacteria to form clusters called biofilms. Scientists at The Scripps Research Institute have unraveled a complex chemical pathway that enables bacteria to form clusters called biofilms. Past research had also revealed that nitric oxide is involved in influencing bacterial biofilm formation. Nitric Oxide Modulates Bacterial Biofilm Formation through a Multicomponent Cyclic-di-GMP Signaling Network. The Scripps Research Institute. "Mystery of bacterial growth and resistance solved: Findings shed light on how bacteria form protective biofilms." ScienceDaily. The Scripps Research Institute. Mystery of bacterial growth and resistance solved: Findings shed light on how bacteria form protective biof...
http://sciencedaily.com/releases/2012/04/120426135128.htm
*  Mankin Lab - Publications
Browse by year: 2014    2013    2012    2011    2010    2009    2008    2007    2006    2005    2004    2003    2002    2001    2000    1999    1998    1997    1996    1995   . Proc Natl Acad Sci USA 111, 9804-9809. 2014 The general mode of translation inhibition by macrolide antibiotics Proc Natl Acad Sci USA 111, 15958–15963. Top. Top. Top. Proc Natl Acad Sci USA 108, 5931-5932. Proc Natl Acad Sci USA 108, 10496-10501. Vázquez-Laslop, N., Ramu, H., Mankin, A. 2011 Nascent peptide in the ribosome exit tunnel affects functional properties of the A-Site of the peptidyl transferase center Mol Cell 41, 321-330. Top. Proc Natl Acad Sci USA 107, 1983-1988. A., Xiong, L., Mankin, A. H D 2010 Structures of the Escherichia coli ribosome with antibiotics bound near the peptidyl transferase center explain spectra of drug action Proc Natl Acad Sci USA 107, 17152-...
http://mankinlab.cpb.uic.edu/cgi-bin/publication.cgi
*  GO:1900911 regulation of olefin biosynthetic process
... Services. Research. Training. Industry. About us. QuickGO A fast browser for Gene Ontology terms and annotations. EBI Databases QuickGO GO:1900911 regulation of olefin biosynthetic process. Search for terms by keyword or ID: apoptosis GO:0006915 Search for proteins by name or accession: tropomyosin P06727. Web Services. Dataset. Term Basket. Term Information ID GO:1900911. Name regulation of olefin biosynthetic process. Ontology Biological Process. Definition Any process that modulates the frequency, rate or extent of olefin biosynthetic process. GONUTS GO:1900911 Wiki Page. Synonyms. Synonyms are alternative words or phrases closely related in meaning to the term name, with indication of the relationship between the name and synonym given by the synonym scope. Click on the icon for more details. Type Synonym. exact regulation of olefin synthesis. exact regulation of olefin biosynthesis. exact regulation of olefin formation. exact regulation of olefin anabolism. Ancestor Chart This chart is interactive; ...
http://ebi.ac.uk/QuickGO/GTerm?id=GO:1900911
*  Albums by Smokey Robinson : Rhapsody
... JavaScript is disabled in your browser settings. rhapsody.com requires JavaScript. Cancel. Free trial. x Music. Apps Devices. Pricing. Sign up. Company Info. Careers. Press Media. Partners. Account. Customer Support. Redeem Coupon. Buy a Gift. 2015 Rhapsody International Inc. ×. Rhapsody App for. Rhapsody International, Inc. Get app. Have the app. Music Apps Devices Pricing Listen Now Try Rhapsody. Sign In. Home. / Music. / Soul/R B. / Motown. / Smokey Robinson. / Facebook. Twitter. Albums by Smokey Robinson. Main Releases. Play. Smokey & Friends. Smokey Robinson. Play. Be Near Me. Smokey Robinson. Play. The Motown Years Live. Smokey Robinson. Play. Ev'ry Man Should Know. Smokey Robinson. Play. The Solo Anthology. Smokey Robinson. Play. The Stripped Mixes. Smokey Robinson. Play. Time Flies When You're Having Fun. Smokey Robinson. Play. Love Songs. Smokey Robinson. Play. Timeless Love. Smokey Robinson. Play. The Definitive Collection. Smokey Robinson. Play. 20th Century Masters: The Millennium Collection....
http://rhapsody.com/artist/smokey-robinson/albums
*  Robinson News | News | News Releases | Robinson Memorial Hospital
-Any- Foundation News Robinson News. Foundation Awards Scholarships Benefitting Employees September 25, 2014 The Robinson Memorial Hospital Foundation is pleased to announce the awarding of 9 scholarships to employees of Robinson Memorial Hospital and Rob... Robinson wins NorthCoast 99 Award 12 years in a row September 4, 2014 Robinson Memorial Hospital again has been named as one of the best 99 places to work in Northeast Ohio by the Employers Resource Council. Osteoporosis event to celebrate healthy aging month September 4, 2014 During Robinson Memorial Hospital’s celebration of healthy aging month, there will be a program on Osteoporosis on Wednesday, September 10 from 6 ... Foundation Awards Student Scholarships July 10, 2014 The Robinson Memorial Hospital Foundation is pleased to announce the award of seven scholarships to children of Robinson Memorial Hospital and Robi... Robinson Wound Care Center Joins Healogics, Inc. to Raise Awareness of Chronic Wounds May 29, 2014 The Robinson Wound Care Center at ...
http://robinsonmemorial.org/main/news-releases/451.aspx?email=1
*  Robinson News | News | News Releases | Robinson Memorial Hospital
-Any- Foundation News Robinson News. Foundation Awards Scholarships Benefitting Employees September 25, 2014 The Robinson Memorial Hospital Foundation is pleased to announce the awarding of 9 scholarships to employees of Robinson Memorial Hospital and Rob... Robinson wins NorthCoast 99 Award 12 years in a row September 4, 2014 Robinson Memorial Hospital again has been named as one of the best 99 places to work in Northeast Ohio by the Employers Resource Council. Osteoporosis event to celebrate healthy aging month September 4, 2014 During Robinson Memorial Hospital’s celebration of healthy aging month, there will be a program on Osteoporosis on Wednesday, September 10 from 6 ... Foundation Awards Student Scholarships July 10, 2014 The Robinson Memorial Hospital Foundation is pleased to announce the award of seven scholarships to children of Robinson Memorial Hospital and Robi... Robinson Wound Care Center Joins Healogics, Inc. to Raise Awareness of Chronic Wounds May 29, 2014 The Robinson Wound Care Center at ...
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*  Robinson News | News | News Releases | Robinson Memorial Hospital
-Any- Foundation News Robinson News. Foundation Awards Scholarships Benefitting Employees September 25, 2014 The Robinson Memorial Hospital Foundation is pleased to announce the awarding of 9 scholarships to employees of Robinson Memorial Hospital and Rob... Robinson wins NorthCoast 99 Award 12 years in a row September 4, 2014 Robinson Memorial Hospital again has been named as one of the best 99 places to work in Northeast Ohio by the Employers Resource Council. Osteoporosis event to celebrate healthy aging month September 4, 2014 During Robinson Memorial Hospital’s celebration of healthy aging month, there will be a program on Osteoporosis on Wednesday, September 10 from 6 ... Foundation Awards Student Scholarships July 10, 2014 The Robinson Memorial Hospital Foundation is pleased to announce the award of seven scholarships to children of Robinson Memorial Hospital and Robi... Robinson Wound Care Center Joins Healogics, Inc. to Raise Awareness of Chronic Wounds May 29, 2014 The Robinson Wound Care Center at ...
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*  Robinson Health Center at Aurora | Robinson Memorial Hospital
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*  Home | Robinson Memorial Hospital
Robinson Memorial Hospital. ABOUT US FOUNDATION VOLUNTEER SPEAKER'S BUREAU PRESS ROOM CONTACT US. Patients Visitors. Medical Services. Nursing. Find a Doctor. Locations. Careers. Patient Portal. Classes Events. Holiday Journeys - Grief Support. Mon, Oct 5, 2015. 2:00 PM - 4:00 PM. Holiday Journeys - Grief Support. Mon, Oct 5, 2015. 6:30 PM - 8:30 PM. Ostomy Support Group. Mon, Oct 5, 2015. 7:00 PM - 9:00 PM. Diabetes Support Group. Tue, Oct 6, 2015. 5:00 PM - 6:00 PM. Tue, Oct 6, 2015. 5:00 PM - 6:30 PM. Robinson In The News Robinson Memorial Hospital is now UH Portage Medical Center. Robinson Health System becomes part of University Hospitals on June 1. Robinson Memorial hosts 21st Annual Nursing Research Day. Robinson Memorial Hospital Foundation Hosts 2015 Robinson Society and Heritage Society Distinguished Giving Recognition. Doctors Receiving Top Scores in Patient Satisfaction Congratulations to the following doctors who rank in the 90th percentile on the Press Ganey Patient Satisfaction Survey. Jessica ...
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*  Rehabilitation | Robinson Memorial Hospital
Rehabilitation. Robinson Memorial Hospital. ABOUT US FOUNDATION VOLUNTEER SPEAKER'S BUREAU PRESS ROOM CONTACT US. Patients Visitors. Medical Services. Locations. Patient Portal. Medical Services. Rehabilitation. Rehabilitation. The Robinson Rehab Center and Sport Clinic works to rehabilitate each patient to his or her maximum potential and works to provide the patient with the necessary skills to maintain his or her progress. Ongoing communication between the patient, therapist and referring physician is the foundation of the center's rehabilitation programs. View Aquatic Classes. Team of Professionals Each therapist strives to stay on top of the latest technology and treatments and passes this knowledge along to patients. All Robinson Rehab Center and Sport Clinic professionals are licensed or certified by the State of Ohio. The center provides:. Aquatic aerobics, aquatic exercise and water arthritis classes in Ravenna. Locations: Robinson Medical Arts Building. 6847 North Chestnut Street Ravenna, Ohio 44266...
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*  Shamim Patel - UK address and phone number - 192.com
... People. All People Businesses Places Electoral Roll Directors. Make sure the biggest decision you'll make is also the best. People. AZ-People. Surname - Patel. Your results for Shamim Patel. Filter Search. Search by name and/or location using the search form at the top of the page. Get access to likely ages Find old friends and family Double check names of spouses and children Trace your family tree Find out who your neighbours are Verify people are who they say they are. Make sure the biggest decision you'll make is also the best. Find out more. previous. 110 Results for Shamim Patel. Looking for Shamim Patel. Below are the results from the UK Electoral Roll and Company Director data. You can narrow your search by adding a location above if you wish. Name Address Other Occupants Electoral Roll Director Info Length of Occupancy Neighbours Property Price. 1 Shamim Patel. Age Guide: 40-44. Reading, Berkshire, RG2 Full Address. View. 2 Shamim R Patel. Age Guide: 30-34. Leicester, Leicestershire, LE5 Full Ad...
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*  Phillip Phillips News, Pictures, and Videos | TMZ.com
Home Celebs Phillip Phillips Phillip Phillips. Idol Managers Claim John Mayer's ex-manager plotted to jack "American Idol" champ Phillip Phillips out from under his contract with the show -- according to a new lawsuit, but the bigger news is Phil is a READ MORE > - 9/21/2015 2:51 PM PDT. Phillip Phillips I Want to be Gone, Gone, Gone From My Idol Contract Phillip Phillips says "American Idol" producers are ruining his career ... American Idol Winner Phillip Phillips Mother Popped for DUI Phillip Phillips' mother Sheryl Phillips -- who appeared on several episodes of "American Idol" during Phillip's championship run last season -- was arrested for DUI earlier today, TMZ has READ MORE > - 964 days ago. Phillip Phillips I LOVE MY FAMILY But ... READ MORE > - 1064 days ago. Phillip Phillips Family Idol Champ s Forcing Us To Sell Pawn Shop Phillip Phillips' family says the singer left them high and dry after winning "American Idol" -- refusing to help them out financially -- and now, they've been forced to sell th...
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*  Shadowing | Robinson Memorial Hospital
Shadowing. Robinson Memorial Hospital. ABOUT US FOUNDATION VOLUNTEER SPEAKER'S BUREAU PRESS ROOM CONTACT US. Patients Visitors. Medical Services. Nursing. Find a Doctor. Locations. Careers. Patient Portal. Home. Volunteer. Shadowing. Shadowing. Robinson Memorial Hospital offers several types of opportunities for short-term observational learning experiences for high school, college and adult students pursuing healthcare careers. The process to apply for a shadow experience depends on the age of the applicant. Note: Robinson Memorial Hospital has a mandatory flu vaccination policy for all employees, volunteers, contracted workers and shadow students. Students wanting to shadow at Robinson Memorial must provide proof of receiving a flu vaccine during current flu season - either by a receipt showing the student's name and detail of service from the provider, an insurance benefits explanation or detailed note from a physician. Students can bring their vaccine documentation the morning of their assigned shadow exp...
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*  [Haskell-cafe] System.Random.Shuffle fix
System.Random.Shuffle fix. System.Random.Shuffle fix friggin friggin frigginfriggins at gmail.com. Previous message: A small display problem using Helium Next message: System.Random.Shuffle fix Messages sorted by:. I was looking for a shuffling algorithm to shuffle mp3-playlists so was very happy to see System.Random.Shuffle: http://hackage.haskell.org/cgi-bin/hackage-scripts/package/random-shuffle-0.0.2 However I get errors,non-exhaustive patterns in function shufleTree or extractTree depending how I call it. Errors are at the bottom. I fixed it but I don't have the math skills to see if I perhaps broke it statistically ... Here is my fix, someone don't remember who, helped me a little : http://hpaste.org/fastcgi/hpaste.fcgi/view?id=2789#a2789 the shuffle at the end is with the fix. *Freet S.shuffle Loading package syb ... linking ... Loading package base-3.0.3.0 ... linking ... Loading package old-locale-1.0.0.1 ... linking ... Loading package old-time-1.0.0.1 ... linking ... Loading package random-1.0.0.1 ...
https://mail.haskell.org/pipermail/haskell-cafe/2009-March/058399.html
*  OriGene - LRRC2 (NM 024512) cDNA Clone
OriGene - LRRC2 NM 024512 cDNA Clone. cDNA Clones TrueORF cDNA Clones H/M/R Viral ORF Clones Destination Vector TrueClone Human TrueClone Mouse Organelle Marker Plasmids MicroRNA Tools Mutant and Variant Clones Plasmid Purification Kits Transfection Reagents Gene Synthesis Service. Home TrueClone LRRC2 Clone. LRRC2 NM 024512 Human cDNA Clone. SC310473 LRRC2 untagged -Human leucine rich repeat containing 2 LRRC2, NM 024512.2, 10ug $680 3 weeks. Reference Data RefSeq: NM 024512.2, NP 078788. Synonyms : leucine-rich repeat-containing 2; leucine rich repeat containing 2. More TrueClone Citations >>. LRRC2 Mouse Clones SKU Description Amount Price Shipping. MC211501 Lrrc2 untagged - Mouse leucine rich repeat containing 2 Lrrc2, 10ug 10 ug $380 3 weeks. MR216368 Lrrc2 Myc-DDK-tagged - Mouse leucine rich repeat containing 2 Lrrc2 10 ug $380 Next day. MG216368 Lrrc2 GFP-tagged - Mouse leucine rich repeat containing 2 Lrrc2, 10ug 10 ug $420 3 weeks. MR216368L1 Lenti ORF clone of Lrrc2 Myc-DDK-tagged - Mouse leucine ri...
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*  JUST LIKE A WOMAN LYRICS - BOB DYLAN
just like a woman lyrics bob dylan sing lyrics bob dylan biography bob dylan albums bob dylan lyrics bob dylan just like a woman lyrics last updated pm sponsored links nobody feels any pain tonight as i stand inside the rain ev rybody knows that baby s got new clothes but lately i see her ribbons and her bows have fallen from her curls she takes just like a woman yes she does she makes love just like a woman yes she does and she aches just like a woman but she breaks just like a little girl queen mary she s my friend yes i believe i ll go see her again nobody has to guess that baby can t be blessed till she finally sees that she s like all the rest with her fog her amphetamine and her pearls she takes just like a woman yes she does she makes love just like a woman yes she does and she aches just like a woman but she breaks just like a little girl it s was raining from the first and i was dying there of thirst so i came in here and your long time curse hurts but what s worse is this pain in here i can t stay i...
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*  Gary Phillips eBooks | epub and pdf downloads | eBookMall
Gary Phillips eBooks. epub and pdf downloads. eBookMall. Search. Sponsored link: What is YOUR Secret Talent. - Take a Free Personality test. Gary Phillips eBooks Epub and PDF format. Gary Phillips eBooks. eBooks found: 15 Occupied Earth: Stories of Aliens, Resistance and Survival at all Costs. Richard Brewer. Gary Phillips Polis Books, October 2015. ISBN: 9781940610528 Format: ePub. List Price: $ 9.99 Our price: $ 7.99. Violent Spring. Gary Phillips mysteriouspress.com, June 2014. ISBN: 9781784087968 Format: ePub. List Price: $ 13.11 Our price: $ 9.99. Perdition, U.S.A. Gary Phillips mysteriouspress.com, June 2014. ISBN: 9781784087975 Format: ePub. List Price: $ 13.11 Our price: $ 9.99. Bad Night Is Falling. Gary Phillips mysteriouspress.com, June 2014. ISBN: 9781784087982 Format: ePub. List Price: $ 13.11 Our price: $ 9.99. Only the Wicked. Gary Phillips mysteriouspress.com, June 2014. ISBN: 9781784087951 Format: ePub. List Price: $ 13.11 Our price: $ 9.99. The Extractors. Gary Phillips Stark Raving Group, M...
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*  "Beyond Stroke" Support Group | Events | Calendar of Events | Robinson Memorial Hospital
beyond stroke support group events calendar of events robinson memorial hospital about us foundation volunteer speaker s bureau press room contact us patients visitors medical services nursing find a doctor locations careers patient portal beyond stroke support group calendar view back to previous page support groups tuesday october pm pm description stroke survivors and caregivers will benefit from information presented by a variety of speakers as well as from sharing and support of their peers for information call jan bahle at location robinson medical arts building rmab n chestnut st ravenna ohio contact healtheducation rmh org phone visitors patient portal key phone numbers visiting hours wheelchair assistance wi fi at robinson memorial hospital locations robinson memorial hospital robinson medical arts building robinson professional center robinson health center at aurora robinson hea...
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*  Deposition of Bobby Phillips States That Erwin Cain Sold Cocaine
... Home About Stories. Featured. BOR Featured. User Stories. Deposition of Bobby Phillips States That Erwin Cain Sold Cocaine. Deposition of Bobby Phillips States That Erwin Cain Sold Cocaine 3. By Burnt Orange Report. on October 19, 2010. Bobby Phillips, Jr. Phillips states that he met Erwin Cain in October of 1977, when he was engaged to Erwin's sister, Gloria Cain. Attorney : Did you ever see Mr. Erwin Cain sell drugs. Phillips : Yeah, I've seen him deliver to his brother-in-law, to his brother, and to other people that came by the house when he was there. Attorney : Do you know where Thomas Cain, Jr. Attorney: which brother would that be. Phillips : Erwin. Attorney: Just to clarify, and let me repeat it's your statement that you have seen, in the past, a man you can identify as Erwin Cain use marijuana. Phillips : Yes. Attorney: Have you, and is it your statement also that you have seen him sell marijuana to someone. Phillips : Yeah. Phillips : It was usually just we used to go over to his h...
http://burntorangereport.com/diary/10880/deposition-of-bobby-phillips-states-that-erwin-cain-sold-cocaine
*  Anthony Robinson | dafont.com
Anthony Robinson. dafont.com. English. Fran ais. Espa ol. Deutsch. Italiano. Portugu s Login. Register. Themes New fonts Authors Top Forum FAQ Submit a font Tools. 4 matching requests on the forum Anthony Robinson 1. 2 3. Anthony Robinson. View profile Send a private message http://www.redbubble.com/people/anfa/portfolio 58 fonts - 4,331,510 downloads 2,129 yesterday. Preview. Fonts 10 20 50 100. Show variants. Size Tiny Small Medium Large Sort by Name Popularity Newer first. More options. Only as. Public domain / GPL / OFL. 100% Free. Free for personal use. Donationware. Shareware. Demo. Unknown Only fonts with. Accents. Euro. Newcastle by Anthony Robinson. in Fancy. Decorative 15,200 downloads 4 yesterday. 100% Free - 2 font files Download Donate to author. Static Buzz by Anthony Robinson. in Fancy. Distorted 104,264 downloads 4 yesterday. 100% Free Download Donate to author. Lasso Of Truth by Anthony Robinson. in Gothic. Modern 5,855 downloads 4 yesterday. 100% Free Download Donate to author. Punched by An...
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DNA sequencer: A DNA sequencer is a scientific instrument used to automate the DNA sequencing process. Given a sample of DNA, a DNA sequencer is used to determine the order of the four bases: G (guanine), C (cytosine), A (adenine) and T (thymine).Coles PhillipsSymmetry element: A symmetry element is a point of reference about which symmetry operations can take place. In particular, symmetry elements can be centers of inversion, axes of rotation and mirror planes.Protein primary structure: The primary structure of a peptide or protein is the linear sequence of its amino acid structural units, and partly comprises its overall biomolecular structure. By convention, the primary structure of a protein is reported starting from the amino-terminal (N) end to the carboxyl-terminal (C) end.Branching order of bacterial phyla (Gupta, 2001): There are several models of the Branching order of bacterial phyla, one of these was proposed in 2001 by Gupta based on conserved indels or protein, termed "protein signatures", an alternative approach to molecular phylogeny. Some problematic exceptions and conflicts are present to these conserved indels, however, they are in agreement with several groupings of classes and phyla.Ligation-independent cloning: Ligation-independent cloning (LIC) is a form of molecular cloning that is able to be performed without the use of restriction endonucleases or DNA ligase. This allows genes that have restriction sites to be cloned without worry of chopping up the insert.Amplified Ribosomal DNA Restriction Analysis: Amplified rDNA (Ribosomal DNA) Restriction Analysis is the extension of the technique of RFLP (restriction fragment length polymorphism) to the gene encoding the small (16s) ribosomal subunit of bacteria. The technique involves an enzymatic amplification using primers directed at the conserved regions at the ends of the 16s gene, followed by digestion using tetracutter Restriction enzymes.CS-BLASTThermal cyclerFerric uptake regulator family: In molecular biology, the ferric uptake regulator (FUR) family of proteins includes metal ion uptake regulator proteins. These are responsible for controlling the intracellular concentration of iron in many bacteria.Open reading frame: In molecular genetics, an open reading frame (ORF) is the part of a reading frame that has the potential to code for a protein or peptide. An ORF is a continuous stretch of codons that do not contain a stop codon (usually UAA, UAG or UGA).Protein subcellular localization prediction: Protein subcellular localization prediction (or just protein localization prediction) involves the computational prediction of where a protein resides in a cell.DNA condensation: DNA condensation refers to the process of compacting DNA molecules in vitro or in vivo. Mechanistic details of DNA packing are essential for its functioning in the process of gene regulation in living systems.Silent mutation: Silent mutations are mutations in DNA that do not significantly alter the phenotype of the organism in which they occur. Silent mutations can occur in non-coding regions (outside of genes or within introns), or they may occur within exons.List of strains of Escherichia coli: Escherichia coli is a well studied bacterium that was first identified by Theodor Escherich, after whom it was later named.Triparental mating: Triparental mating is a form of Bacterial conjugation where a conjugative plasmid present in one bacterial strain assists the transfer of a mobilizable plasmid present in a second bacterial strain into a third bacterial strain. Plasmids are introduced into bacteria for such purposes as transformation, cloning, or transposon mutagenesis.ParaHox: The ParaHox gene cluster is an array of homeobox genes (involved in morphogenesis, the regulation of patterns of anatomical development) from the Gsx, Xlox (Pdx) and Cdx gene families.Community Fingerprinting: Community fingerprinting refers to a set of molecular biology techniques that can be used to quickly profile the diversity of a microbial community. Rather than directly identifying or counting individual cells in an environmental sample, these techniques show how many variants of a gene are present.Transfer-messenger RNA: Transfer-messenger RNA (abbreviated tmRNA, also known as 10Sa RNA and by its genetic name SsrA) is a bacterial RNA molecule with dual tRNA-like and messenger RNA-like properties. The tmRNA forms a ribonucleoprotein complex (tmRNP) together with Small Protein B (SmpB), Elongation Factor Tu (EF-Tu), and ribosomal protein S1.Genetic variation: right|thumbMature messenger RNA: Mature messenger RNA, often abbreviated as mature mRNA is a eukaryotic RNA transcript that has been spliced and processed and is ready for translation in the course of protein synthesis. Unlike the eukaryotic RNA immediately after transcription known as precursor messenger RNA, it consists exclusively of exons, with all introns removed.Chromosome regionsLibrary (biology): In molecular biology, a library is a collection of DNA fragments that is stored and propagated in a population of micro-organisms through the process of molecular cloning. There are different types of DNA libraries, including cDNA libraries (formed from reverse-transcribed RNA), genomic libraries (formed from genomic DNA) and randomized mutant libraries (formed by de novo gene synthesis where alternative nucleotides or codons are incorporated).Eukaryotic transcription: Eukaryotic transcription is the elaborate process that eukaryotic cells use to copy genetic information stored in DNA into units of RNA replica. Gene transcription occurs in both eukaryotic and prokaryotic cells.Phenotype microarray: The phenotype microarray approach is a technology for high-throughput phenotyping of cells.Gemmatimonadetes: The Gemmatimonadetes are a family of bacteria, given their own phylum (Gemmatimonadetes). This bacterium makes up about 2% of soil bacterial communities and has been identified as one of the top nine phyla found in soils; yet, there are currently only six cultured isolates.Direct repeat: Direct repeats are a type of genetic sequence that consists of two or more repeats of a specific sequence.Molar mass distribution: In linear polymers the individual polymer chains rarely have exactly the same degree of polymerization and molar mass, and there is always a distribution around an average value. The molar mass distribution (or molecular weight distribution) in a polymer describes the relationship between the number of moles of each polymer species (Ni) and the molar mass (Mi) of that species.Restriction fragment: A restriction fragment is a DNA fragment resulting from the cutting of a DNA strand by a restriction enzyme (restriction endonucleases), a process called restriction. Each restriction enzyme is highly specific, recognising a particular short DNA sequence, or restriction site, and cutting both DNA strands at specific points within this site.Composite transposon: A composite transposon is similar in function to simple transposons and Insertion Sequence (IS) elements in that it has protein coding DNA segments flanked by inverted, repeated sequences that can be recognized by transposase enzymes. A composite transposon, however, is flanked by two separate IS elements which may or may not be exact replicas.Molecular evolution: Molecular evolution is a change in the sequence composition of cellular molecules such as DNA, RNA, and proteins across generations. The field of molecular evolution uses principles of evolutionary biology and population genetics to explain patterns in these changes.Alternative splicing: Alternative splicing is a regulated process during gene expression that results in a single gene coding for multiple proteins. In this process, particular exons of a gene may be included within or excluded from the final, processed messenger RNA (mRNA) produced from that gene.Margaret Jope: Margaret Jope (1913–2004) was a Scottish biochemist, born as Henrietta Margaret Halliday in Peterhead, Scotland.Heptadecanoic acidAmplified fragment length polymorphismMannose receptor: The mannose receptor (Cluster of Differentiation 206) is a C-type lectin primarily present on the surface of macrophages and immature dendritic cells, but is also expressed on the surface of skin cells such as human dermal fibroblasts and keratinocytes. It is the first member of a family of endocytic receptors that includes Endo180 (CD280), M-type PLA2R, and DEC-205 (CD205).DNA binding site: DNA binding sites are a type of binding site found in DNA where other molecules may bind. DNA binding sites are distinct from other binding sites in that (1) they are part of a DNA sequence (e.GC box: In molecular biology, a GC box is a distinct pattern of nucleotides found in the promoter region of some eukaryotic genes upstream of the TATA box and approximately 110 bases upstream from the transcription initiation site. It has a consensus sequence GGGCGG which is position dependent and orientation independent.Codon Adaptation Index: The Codon Adaptation Index (CAI) is the most widespread technique for analyzing Codon usage bias. As opposed to other measures of codon usage bias, such as the 'effective number of codons' (Nc), which measure deviation from a uniform bias (null hypothesis), CAI measures the deviation of a given protein coding gene sequence with respect to a reference set of genes.High-performance liquid chromatography: High-performance liquid chromatography (HPLC; formerly referred to as high-pressure liquid chromatography), is a technique in analytical chemistry used to separate, identify, and quantify each component in a mixture. It relies on pumps to pass a pressurized liquid solvent containing the sample mixture through a column filled with a solid adsorbent material.Cardiac function curve: A cardiac function curve is a graph showing the relationship between right atrial pressure (x-axis) and cardiac output (y-axis).Allele-specific oligonucleotide: An allele-specific oligonucleotide (ASO) is a short piece of synthetic DNA complementary to the sequence of a variable target DNA. It acts as a probe for the presence of the target in a Southern blot assay or, more commonly, in the simpler Dot blot assay.Beef cattle: Beef cattle are cattle raised for meat production (as distinguished from dairy cattle, used for milk production). The meat of adult cattle is known as beef.Operon: In genetics, an operon is a functioning unit of genomic DNA containing a cluster of genes under the control of a single promoter. The genes are transcribed together into an mRNA strand and either translated together in the cytoplasm, or undergo trans-splicing to create monocistronic mRNAs that are translated separately, i.Point mutationDeep chlorophyll maximum: A deep chlorophyll maximum (DCM) is a subsurface maximum in the concentration of chlorophyll in the ocean or a lake. A DCM is not always present--sometimes there is more chlorophyll at the surface than at any greater depth--but it is a common feature of most aquatic ecosystems.Infinite alleles model: The infinite alleles model is a mathematical model for calculating genetic mutations. The Japanese geneticist Motoo Kimura and American geneticist James F.Clavibacter michiganensis: Clavibacter michiganensis is an aerobic non-sporulating Gram-positive plant pathogenic actinomycete that currently constitutes the only species within the genus Clavibacter. The other former Clavibacter species have been reclassified to genera Leifsonia, Rathayibacter and Curtobacterium.Recombination (cosmology): In cosmology, recombination refers to the epoch at which charged electrons and protons first became bound to form electrically neutral hydrogen atoms.Note that the term recombination is a misnomer, considering that it represents the first time that electrically neutral hydrogen formed.Proteinogenic amino acid: Proteinogenic amino acids are amino acids that are precursors to proteins, and are incorporated into proteins cotranslationally — that is, during translation. There are 23 proteinogenic amino acids in prokaryotes (including N-Formylmethionine, mainly used to initiate protein synthesis and often removed afterward), but only 21 are encoded by the nuclear genes of eukaryotes.YjdF RNA motifPelagibacter ubique: Pelagibacter, with the single species P. ubique, was isolated in 2002 and given a specific name, although it has not yet been validly published according to the bacteriological code.Nucleic acid structure: Nucleic acid structure refers to the structure of nucleic acids such as DNA and RNA. Chemically speaking, DNA and RNA are very similar.Fecal coliform: A fecal coliform (British: faecal coliform) is a facultatively anaerobic, rod-shaped, gram-negative, non-sporulating bacterium. Coliform bacteria generally originate in the intestines of warm-blooded animals.Lattice protein: Lattice proteins are highly simplified computer models of proteins which are used to investigate protein folding.Nankai Trough gas hydrate site: Nankai Methane Hydrate Site (or Japanese Methane Hydrate R&D Program at Nankai, Nankai Trough Methane Hydrate Site) is located in the Nankai Trough, Japan.Virulence: Virulence is, by MeSH definition, the degree of pathogenicity within a group or species of parasites as indicated by case fatality rates and/or the ability of the organism to invade the tissues of the host. The pathogenicity of an organism - its ability to cause disease - is determined by its virulence factors.Database of protein conformational diversity: The Database of protein conformational diversity (PCDB) is a database of diversity of protein tertiary structures within protein domains as determined by X-ray crystallography. Proteins are inherently flexible and this database collects information on this subject for use in molecular research.

(1/46886) Novel regulation of the homeotic gene Scr associated with a crustacean leg-to-maxilliped appendage transformation.

Homeotic genes are known to be involved in patterning morphological structures along the antero-posterior axis of insects and vertebrates. Because of their important roles in development, changes in the function and expression patterns of homeotic genes may have played a major role in the evolution of different body plans. For example, it has been proposed that during the evolution of several crustacean lineages, changes in the expression patterns of the homeotic genes Ultrabithorax and abdominal-A have played a role in transformation of the anterior thoracic appendages into mouthparts termed maxillipeds. This homeotic-like transformation is recapitulated at the late stages of the direct embryonic development of the crustacean Porcellio scaber (Oniscidea, Isopoda). Interestingly, this morphological change is associated with apparent novelties both in the transcriptional and post-transcriptional regulation of the Porcellio scaber ortholog of the Drosophila homeotic gene, Sex combs reduced (Scr). Specifically, we find that Scr mRNA is present in the second maxillary segment and the first pair of thoracic legs (T1) in early embryos, whereas protein accumulates only in the second maxillae. In later stages, however, high levels of SCR appear in the T1 legs, which correlates temporally with the transformation of these appendages into maxillipeds. Our observations provide further insight into the process of the homeotic leg-to-maxilliped transformation in the evolution of crustaceans and suggest a novel regulatory mechanism for this process in this group of arthropods.  (+info)

(2/46886) Mrj encodes a DnaJ-related co-chaperone that is essential for murine placental development.

We have identified a novel gene in a gene trap screen that encodes a protein related to the DnaJ co-chaperone in E. coli. The gene, named Mrj (mammalian relative of DnaJ) was expressed throughout development in both the embryo and placenta. Within the placenta, expression was particularly high in trophoblast giant cells but moderate levels were also observed in trophoblast cells of the chorion at embryonic day 8.5, and later in the labyrinth which arises from the attachment of the chorion to the allantois (a process called chorioallantoic fusion). Insertion of the ROSAbetageo gene trap vector into the Mrj gene created a null allele. Homozygous Mrj mutants died at mid-gestation due to a failure of chorioallantoic fusion at embryonic day 8.5, which precluded formation of the mature placenta. At embryonic day 8.5, the chorion in mutants was morphologically normal and expressed the cell adhesion molecule beta4 integrin that is known to be required for chorioallantoic fusion. However, expression of the chorionic trophoblast-specific transcription factor genes Err2 and Gcm1 was significantly reduced. The mutants showed no abnormal phenotypes in other trophoblast cell types or in the embryo proper. This study indicates a previously unsuspected role for chaperone proteins in placental development and represents the first genetic analysis of DnaJ-related protein function in higher eukaryotes. Based on a survey of EST databases representing different mouse tissues and embryonic stages, there are 40 or more DnaJ-related genes in mammals. In addition to Mrj, at least two of these genes are also expressed in the developing mouse placenta. The specificity of the developmental defect in Mrj mutants suggests that each of these genes may have unique tissue and cellular activities.  (+info)

(3/46886) Requirement of a novel gene, Xin, in cardiac morphogenesis.

A novel gene, Xin, from chick (cXin) and mouse (mXin) embryonic hearts, may be required for cardiac morphogenesis and looping. Both cloned cDNAs have a single open reading frame, encoding proteins with 2,562 and 1,677 amino acids for cXin and mXin, respectively. The derived amino acid sequences share 46% similarity. The overall domain structures of the predicted cXin and mXin proteins, including proline-rich regions, 16 amino acid repeats, DNA-binding domains, SH3-binding motifs and nuclear localization signals, are highly conserved. Northern blot analyses detect a single message of 8.9 and 5.8 kilo base (kb) from both cardiac and skeletal muscle of chick and mouse, respectively. In situ hybridization reveals that the cXin gene is specifically expressed in cardiac progenitor cells of chick embryos as early as stage 8, prior to heart tube formation. cXin continues to be expressed in the myocardium of developing hearts. By stage 15, cXin expression is also detected in the myotomes of developing somites. Immunofluorescence microscopy reveals that the mXin protein is colocalized with N-cadherin and connexin-43 in the intercalated discs of adult mouse hearts. Incubation of stage 6 chick embryos with cXin antisense oligonucleotides results in abnormal cardiac morphogenesis and an alteration of cardiac looping. The myocardium of the affected hearts becomes thickened and tends to form multiple invaginations into the heart cavity. This abnormal cellular process may account in part for the abnormal looping. cXin expression can be induced by bone morphogenetic protein (BMP) in explants of anterior medial mesoendoderm from stage 6 chick embryos, a tissue that is normally non-cardiogenic. This induction occurs following the BMP-mediated induction of two cardiac-restricted transcription factors, Nkx2.5 and MEF2C. Furthermore, either MEF2C or Nkx2.5 can transactivate a luciferase reporter driven by the mXin promoter in mouse fibroblasts. These results suggest that Xin may participate in a BMP-Nkx2.5-MEF2C pathway to control cardiac morphogenesis and looping.  (+info)

(4/46886) Characterization of an amphioxus paired box gene, AmphiPax2/5/8: developmental expression patterns in optic support cells, nephridium, thyroid-like structures and pharyngeal gill slits, but not in the midbrain-hindbrain boundary region.

On the basis of developmental gene expression, the vertebrate central nervous system comprises: a forebrain plus anterior midbrain, a midbrain-hindbrain boundary region (MHB) having organizer properties, and a rhombospinal domain. The vertebrate MHB is characterized by position, by organizer properties and by being the early site of action of Wnt1 and engrailed genes, and of genes of the Pax2/5/8 subfamily. Wada and others (Wada, H., Saiga, H., Satoh, N. and Holland, P. W. H. (1998) Development 125, 1113-1122) suggested that ascidian tunicates have a vertebrate-like MHB on the basis of ascidian Pax258 expression there. In another invertebrate chordate, amphioxus, comparable gene expression evidence for a vertebrate-like MHB is lacking. We, therefore, isolated and characterized AmphiPax2/5/8, the sole member of this subfamily in amphioxus. AmphiPax2/5/8 is initially expressed well back in the rhombospinal domain and not where a MHB would be expected. In contrast, most of the other expression domains of AmphiPax2/5/8 correspond to expression domains of vertebrate Pax2, Pax5 and Pax8 in structures that are probably homologous - support cells of the eye, nephridium, thyroid-like structures and pharyngeal gill slits; although AmphiPax2/5/8 is not transcribed in any structures that could be interpreted as homologues of vertebrate otic placodes or otic vesicles. In sum, the developmental expression of AmphiPax2/5/8 indicates that the amphioxus central nervous system lacks a MHB resembling the vertebrate isthmic region. Additional gene expression data for the developing ascidian and amphioxus nervous systems would help determine whether a MHB is a basal chordate character secondarily lost in amphioxus. The alternative is that the MHB is a vertebrate innovation.  (+info)

(5/46886) Regulation of body length and male tail ray pattern formation of Caenorhabditis elegans by a member of TGF-beta family.

We have identified a new member of the TGF-beta superfamily, CET-1, from Caenorhabditis elegans, which is expressed in the ventral nerve cord and other neurons. cet-1 null mutants have shortened bodies and male tail abnormal phenotype resembling sma mutants, suggesting cet-1, sma-2, sma-3 and sma-4 share a common pathway. Overexpression experiments demonstrated that cet-1 function requires wild-type sma genes. Interestingly, CET-1 appears to affect body length in a dose-dependent manner. Heterozygotes for cet-1 displayed body lengths ranging between null mutant and wild type, and overexpression of CET-1 in wild-type worms elongated body length close to lon mutants. In male sensory ray patterning, lack of cet-1 function results in ray fusions. Epistasis analysis revealed that mab-21 lies downstream and is negatively regulated by the cet-1/sma pathway in the male tail. Our results show that cet-1 controls diverse biological processes during C. elegans development probably through different target genes.  (+info)

(6/46886) Molecular cloning and epitope analysis of the peanut allergen Ara h 3.

Peanut allergy is a significant IgE-mediated health problem because of the increased prevalence, potential severity, and chronicity of the reaction. Following our characterization of the two peanut allergens Ara h 1 and Ara h 2, we have isolated a cDNA clone encoding a third peanut allergen, Ara h 3. The deduced amino acid sequence of Ara h 3 shows homology to 11S seed-storage proteins. The recombinant form of this protein was expressed in a bacterial system and was recognized by serum IgE from approximately 45% of our peanut-allergic patient population. Serum IgE from these patients and overlapping, synthetic peptides were used to map the linear, IgE-binding epitopes of Ara h 3. Four epitopes, between 10 and 15 amino acids in length, were found within the primary sequence, with no obvious sequence motif shared by the peptides. One epitope is recognized by all Ara h 3-allergic patients. Mutational analysis of the epitopes revealed that single amino acid changes within these peptides could lead to a reduction or loss of IgE binding. By determining which amino acids are critical for IgE binding, it might be possible to alter the Ara h 3 cDNA to encode a protein with a reduced IgE-binding capacity. These results will enable the design of improved diagnostic and therapeutic approaches for food-hypersensitivity reactions.  (+info)

(7/46886) TIF1gamma, a novel member of the transcriptional intermediary factor 1 family.

We report the cloning and characterization of a novel member of the Transcriptional Intermediary Factor 1 (TIF1) gene family, human TIF1gamma. Similar to TIF1alpha and TIF1beta, the structure of TIF1beta is characterized by multiple domains: RING finger, B boxes, Coiled coil, PHD/TTC, and bromodomain. Although structurally related to TIF1alpha and TIF1beta, TIF1gamma presents several functional differences. In contrast to TIF1alpha, but like TIF1beta, TIF1 does not interact with nuclear receptors in yeast two-hybrid or GST pull-down assays and does not interfere with retinoic acid response in transfected mammalian cells. Whereas TIF1alpha and TIF1beta were previously found to interact with the KRAB silencing domain of KOX1 and with the HP1alpha, MODI (HP1beta) and MOD2 (HP1gamma) heterochromatinic proteins, suggesting that they may participate in a complex involved in heterochromatin-induced gene repression, TIF1gamma does not interact with either the KRAB domain of KOX1 or the HP1 proteins. Nevertheless, TIF1gamma, like TIF1alpha and TIF1beta, exhibits a strong silencing activity when tethered to a promoter. Since deletion of a novel motif unique to the three TIF1 proteins, called TIF1 signature sequence (TSS), abrogates transcriptional repression by TIF1gamma, this motif likely participates in TIF1 dependent repression.  (+info)

(8/46886) Detailed methylation analysis of the glutathione S-transferase pi (GSTP1) gene in prostate cancer.

Glutathione-S-Transferases (GSTs) comprise a family of isoenzymes that provide protection to mammalian cells against electrophilic metabolites of carcinogens and reactive oxygen species. Previous studies have shown that the CpG-rich promoter region of the pi-class gene GSTP1 is methylated at single restriction sites in the majority of prostate cancers. In order to understand the nature of abnormal methylation of the GSTP1 gene in prostate cancer we undertook a detailed analysis of methylation at 131 CpG sites spanning the promoter and body of the gene. Our results show that DNA methylation is not confined to specific CpG sites in the promoter region of the GSTP1 gene but is extensive throughout the CpG island in prostate cancer cells. Furthermore we found that both alleles are abnormally methylated in this region. In normal prostate tissue, the entire CpG island was unmethylated, but extensive methylation was found outside the island in the body of the gene. Loss of GSTP1 expression correlated with DNA methylation of the CpG island in both prostate cancer cell lines and cancer tissues whereas methylation outside the CpG island in normal prostate tissue appeared to have no effect on gene expression.  (+info)


What is the nutritional analysis for 15% drained ground beef?


What is the nutritional analysis for 15% drained ground beef?
I've had this sort of problem before (though rarely); that is, finding basic info on something as ubiquitous as drained ground beef. But go ahead and google for nutritional analysis "drained ground beef". 9 entries! It's insane! 
So does anyone know what it is?
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The USDA does wonderful things and this is no exception:
http://www.fns.usda.gov/fdd/facts/schfacts/intro-schoolfacts.htm


How should I go about getting a semen analysis?


My wife and I have been trying for a pregnancy for a year now.  I want to have a semen analysis done to help shed some light on why we haven't been successful.  Should I talk to my doctor about this or do I need to go to a special clinic?
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You can go a number of places for a semen analysis. Since you and your wife have been trying to conceive for over a year, you should make an appointment for the both of you with a Fertility Specialist. He/She will do a semen analysis for you along with other tests on you and your spouse. This is the route that I would recommend.

If you do not want to pay the money to see a specialist, you can speak with your regular doctor, see a urologist or go to a free clinic. Each should be able to help you.

Good Luck.


What is the nutritional analysis for 15% drained ground beef?


What is the nutritional analysis for 15% drained ground beef?
I've had this sort of problem before (though rarely); that is, finding basic info on something as ubiquitous as drained ground beef. But go ahead and google for nutritional analysis "drained ground beef". 9 entries! It's insane! 
So does anyone know what it is?
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If you take the nutritional facts of bologna beef you could say that 15%ground beef contains:

15% protein
81% fats
4% carbs


http://www.nutritiondata.com/facts-C00001-01c20LN.html ... this is for 14%


Is it possible to put DNA from another women into one womens egg to cause a pregnancy?


I know they can use DNA form sperm for In vitro fertilization (IVF) but can they use DNA from the egg of another female?
Jill that is Very helpful, do you have a LINK or anything more about this, because i would appreciate that.
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It's technically possible (scientists fertilized mouse eggs using non-sperm DNA as much as 10 years ago), but there are complications that make it impractical.  The sperm doesn't contain only DNA, but it also contains enzymes and chemicals that cause the process of fertilization to take place (the enzymes tell the egg to "get rid of" one copy of it's DNA, then join the sperm and egg DNA, then kick off the process of cell division).  Without those enzymes and chemicals, you'd have a cell containing 2 different sets of DNA, but nothing would happen next.

That being said, scientists are making great progress in combining the DNA of 2 eggs and THEN fertilizing the resulting egg with a sperm -- this is useful if you have a lesbian couple who wants to have a child that is biologically related to both of them.


What happens when all parties are swabbed for a DNA test and the tips are touching? Could he be the dad?


My brother went to Walgreen's and bought a DNA test in a box, swabbed all parties and put all the swabs in the same envelope and they were all touching one another. The results came back and one was in the 92 percentile and the other was in the 96 percentile.  Is there a possibility that the swabs were contaminated with his DNA when they touched in the envelope? Does he need to take this DNA test the right way through a reputable company?
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Unless he stuck them into his mouth/nose/ other orifices or handled the tips with his hands he can't have contaminated them. It's more likely that they contaminated each other. The swabs should have been seperatly preserved, I'm afraid your results are useless


is it possible for a DNA test to be wrong and the child look just like the alleged father?


Is it possible to have a swab DNA test done and it come back that the alleged father is not the father. However my child looks just like him, as if he spit out. Can someone please help put a stop to my madness.
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I think that it is highly ulikely, but i did see a tv show of a women who had several kids and her dna did not match any other her kids. She was accused of not being the mother and threatened to have the kids taken away from her until she was pregnant again and they watched her give birth to the child and then tested the newborn baby and the dna did not match the mother but obviously she was the mother.. i'm sure possibly it could happen with a father too, but probably super super rare.


Can a sisters DNA determine the paternity of her brothers child?


Just curious since they are brother and sister, how likely will the DNA match. If the potential father isn't available for the testing, could his sister's DNA be used to determine whether the child is biologically his?
Also where can you find tests that will do this?
Also where can you find tests that will do this?
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If the father isn't available, the next best choice would be to test the father's parents (the baby's grandparents). If they aren't available, then a sister or brother of the father could be tested.

This is called an "avuncular" test. IDENTIGENE does these tests for $495. You can call them at 1-888-404-GENE or find them online at www.identigene.com.


How long does it take to get results from a semen analysis?


My husband had to do a semen analysis and I was wondering if anyone knows how long it takes to get the results back.  He did it two days ago.
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He should be able to call and get the results now.  They have to do the analysis almost immediately while the sperm are still alive and "fresh".  Some places only call if the results are abnormal, some wait until the two of you come in together on your next visit.  But they should have the results by now and he should be able to call and get them today.