Proteomics: The systematic study of the complete complement of proteins (PROTEOME) of organisms.Proteome: The protein complement of an organism coded for by its genome.Mass Spectrometry: An analytical method used in determining the identity of a chemical based on its mass using mass analyzers/mass spectrometers.Tandem Mass Spectrometry: A mass spectrometry technique using two (MS/MS) or more mass analyzers. With two in tandem, the precursor ions are mass-selected by a first mass analyzer, and focused into a collision region where they are then fragmented into product ions which are then characterized by a second mass analyzer. A variety of techniques are used to separate the compounds, ionize them, and introduce them to the first mass analyzer. For example, for in GC-MS/MS, GAS CHROMATOGRAPHY-MASS SPECTROMETRY is involved in separating relatively small compounds by GAS CHROMATOGRAPHY prior to injecting them into an ionization chamber for the mass selection.Electrophoresis, Gel, Two-Dimensional: Electrophoresis in which a second perpendicular electrophoretic transport is performed on the separate components resulting from the first electrophoresis. This technique is usually performed on polyacrylamide gels.Chromatography, Liquid: Chromatographic techniques in which the mobile phase is a liquid.Databases, Protein: Databases containing information about PROTEINS such as AMINO ACID SEQUENCE; PROTEIN CONFORMATION; and other properties.Isotope Labeling: Techniques for labeling a substance with a stable or radioactive isotope. It is not used for articles involving labeled substances unless the methods of labeling are substantively discussed. Tracers that may be labeled include chemical substances, cells, or microorganisms.Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization: A mass spectrometric technique that is used for the analysis of large biomolecules. Analyte molecules are embedded in an excess matrix of small organic molecules that show a high resonant absorption at the laser wavelength used. The matrix absorbs the laser energy, thus inducing a soft disintegration of the sample-matrix mixture into free (gas phase) matrix and analyte molecules and molecular ions. In general, only molecular ions of the analyte molecules are produced, and almost no fragmentation occurs. This makes the method well suited for molecular weight determinations and mixture analysis.Proteins: Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.Protein Array Analysis: Ligand-binding assays that measure protein-protein, protein-small molecule, or protein-nucleic acid interactions using a very large set of capturing molecules, i.e., those attached separately on a solid support, to measure the presence or interaction of target molecules in the sample.Computational Biology: A field of biology concerned with the development of techniques for the collection and manipulation of biological data, and the use of such data to make biological discoveries or predictions. This field encompasses all computational methods and theories for solving biological problems including manipulation of models and datasets.Peptides: Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.Software: Sequential operating programs and data which instruct the functioning of a digital computer.Sequence Analysis, Protein: A process that includes the determination of AMINO ACID SEQUENCE of a protein (or peptide, oligopeptide or peptide fragment) and the information analysis of the sequence.Peptide Mapping: Analysis of PEPTIDES that are generated from the digestion or fragmentation of a protein or mixture of PROTEINS, by ELECTROPHORESIS; CHROMATOGRAPHY; or MASS SPECTROMETRY. The resulting peptide fingerprints are analyzed for a variety of purposes including the identification of the proteins in a sample, GENETIC POLYMORPHISMS, patterns of gene expression, and patterns diagnostic for diseases.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Reproducibility of Results: The statistical reproducibility of measurements (often in a clinical context), including the testing of instrumentation or techniques to obtain reproducible results. The concept includes reproducibility of physiological measurements, which may be used to develop rules to assess probability or prognosis, or response to a stimulus; reproducibility of occurrence of a condition; and reproducibility of experimental results.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Genomics: The systematic study of the complete DNA sequences (GENOME) of organisms.Algorithms: A procedure consisting of a sequence of algebraic formulas and/or logical steps to calculate or determine a given task.Protein Processing, Post-Translational: Any of various enzymatically catalyzed post-translational modifications of PEPTIDES or PROTEINS in the cell of origin. These modifications include carboxylation; HYDROXYLATION; ACETYLATION; PHOSPHORYLATION; METHYLATION; GLYCOSYLATION; ubiquitination; oxidation; proteolysis; and crosslinking and result in changes in molecular weight and electrophoretic motility.Gene Expression Profiling: The determination of the pattern of genes expressed at the level of GENETIC TRANSCRIPTION, under specific circumstances or in a specific cell.Biological Markers: Measurable and quantifiable biological parameters (e.g., specific enzyme concentration, specific hormone concentration, specific gene phenotype distribution in a population, presence of biological substances) which serve as indices for health- and physiology-related assessments, such as disease risk, psychiatric disorders, environmental exposure and its effects, disease diagnosis, metabolic processes, substance abuse, pregnancy, cell line development, epidemiologic studies, etc.Spectrometry, Mass, Electrospray Ionization: A mass spectrometry technique used for analysis of nonvolatile compounds such as proteins and macromolecules. The technique involves preparing electrically charged droplets from analyte molecules dissolved in solvent. The electrically charged droplets enter a vacuum chamber where the solvent is evaporated. Evaporation of solvent reduces the droplet size, thereby increasing the coulombic repulsion within the droplet. As the charged droplets get smaller, the excess charge within them causes them to disintegrate and release analyte molecules. The volatilized analyte molecules are then analyzed by mass spectrometry.Two-Dimensional Difference Gel Electrophoresis: Methods of comparing two or more samples on the same two-dimensional gel electrophoresis gel.Protein Interaction Mapping: Methods for determining interaction between PROTEINS.Metabolomics: The systematic identification and quantitation of all the metabolic products of a cell, tissue, organ, or organism under varying conditions. The METABOLOME of a cell or organism is a dynamic collection of metabolites which represent its net response to current conditions.Chemical Fractionation: Separation of a mixture in successive stages, each stage removing from the mixture some proportion of one of the substances, for example by differential solubility in water-solvent mixtures. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Systems Biology: Comprehensive, methodical analysis of complex biological systems by monitoring responses to perturbations of biological processes. Large scale, computerized collection and analysis of the data are used to develop and test models of biological systems.Metabolic Networks and Pathways: Complex sets of enzymatic reactions connected to each other via their product and substrate metabolites.Database Management Systems: Software designed to store, manipulate, manage, and control data for specific uses.Blood Proteins: Proteins that are present in blood serum, including SERUM ALBUMIN; BLOOD COAGULATION FACTORS; and many other types of proteins.Oxygen Isotopes: Stable oxygen atoms that have the same atomic number as the element oxygen, but differ in atomic weight. O-17 and 18 are stable oxygen isotopes.User-Computer Interface: The portion of an interactive computer program that issues messages to and receives commands from a user.Protein Interaction Maps: Graphs representing sets of measurable, non-covalent physical contacts with specific PROTEINS in living organisms or in cells.Search Engine: Software used to locate data or information stored in machine-readable form locally or at a distance such as an INTERNET site.Internet: A loose confederation of computer communication networks around the world. The networks that make up the Internet are connected through several backbone networks. The Internet grew out of the US Government ARPAnet project and was designed to facilitate information exchange.Cluster Analysis: A set of statistical methods used to group variables or observations into strongly inter-related subgroups. In epidemiology, it may be used to analyze a closely grouped series of events or cases of disease or other health-related phenomenon with well-defined distribution patterns in relation to time or place or both.Tumor Markers, Biological: Molecular products metabolized and secreted by neoplastic tissue and characterized biochemically in cells or body fluids. They are indicators of tumor stage and grade as well as useful for monitoring responses to treatment and predicting recurrence. Many chemical groups are represented including hormones, antigens, amino and nucleic acids, enzymes, polyamines, and specific cell membrane proteins and lipids.Complex Mixtures: Mixtures of many components in inexact proportions, usually natural, such as PLANT EXTRACTS; VENOMS; and MANURE. These are distinguished from DRUG COMBINATIONS which have only a few components in definite proportions.Bacterial Proteins: Proteins found in any species of bacterium.Blotting, Western: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.Neoplasm Proteins: Proteins whose abnormal expression (gain or loss) are associated with the development, growth, or progression of NEOPLASMS. Some neoplasm proteins are tumor antigens (ANTIGENS, NEOPLASM), i.e. they induce an immune reaction to their tumor. Many neoplasm proteins have been characterized and are used as tumor markers (BIOMARKERS, TUMOR) when they are detectable in cells and body fluids as monitors for the presence or growth of tumors. Abnormal expression of ONCOGENE PROTEINS is involved in neoplastic transformation, whereas the loss of expression of TUMOR SUPPRESSOR PROTEINS is involved with the loss of growth control and progression of the neoplasm.Models, Biological: Theoretical representations that simulate the behavior or activity of biological processes or diseases. For disease models in living animals, DISEASE MODELS, ANIMAL is available. Biological models include the use of mathematical equations, computers, and other electronic equipment.

*  Proteomics Europe | AllConferences.com
Proteomics and Functional Genomics Group,. University of Liverpool. Dolores Cahill. Professor of Translational Science,. Conway ...
http://allconferences.com/conferences/2009/20090203054637
*  BRIEF-Proteomics International Laboratories says Diabetic Kidney Disease patent...
Dec 19 (Reuters) - Proteomics International Laboratories Ltd : * Granted a patent in Russian Federation for diagnostic and ... BRIEF-Proteomics International Laboratories says Diabetic Kidney Disease patent granted in Russia. ... BRIEF-Proteomics International Laboratories says Diabetic Kidney Disease patent granted in Russia ...
http://in.reuters.com/article/idINFWN1EB0UG
*  Proteomics and the stressful life of lactobacilli - Hussain - 2013 - FEMS...
Proteomics and the stressful life of lactobacilli. Authors. *. Malik A. Hussain,. Corresponding author. *Department of Wine, ... Since the postgenomic era began, proteomics has become the first choice among research approaches available for environmental ... The latest developments in the applications of proteomics to understand physiological changes in Lactobacillus species under ...
http://onlinelibrary.wiley.com/doi/10.1111/1574-6968.12274/abstract
*  Application of proteomics technology in adipocyte biology - Molecular...
In this review novel findings obtained with adipo-proteomics studies are highlighted and the relevance of proteomics ... Application of proteomics technology in adipocyte biology J. Renes and E. Mariman, Mol. BioSyst., 2013, 9, 1076 DOI: 10.1039/ ... Independent proteomics studies have contributed significantly to further insight into adipocyte biology, particularly with ... This article is part of the themed collection: Molecular BioSystems 2013 Proteomics ...
http://pubs.rsc.org/en/Content/ArticleLanding/2013/MB/c3mb25596d
*  Introduction to Computational Proteomics
Proteomics Is the Subject Area "Proteomics" applicable to this article? Yes. No. ...
http://journals.plos.org/ploscompbiol/article?id=10.1371/journal.pcbi.0030114
*  Citation Machine: Bba Proteins And Proteomics format citation generator for...
Cite your music in BBA - Proteins and Proteomics format for free. ... Generate BBA - Proteins and Proteomics citations for Musics. In ...
http://citationmachine.net/bba-proteins-and-proteomics/cite-a-music/manual
*  What Can Proteomics Tell Us About Platelets? | Circulation Research
Summary of 47 platelet proteomics studies. A, Platelet proteomics has been used to characterize the global proteome,21,46,52,53 ... What Can Proteomics Tell Us About Platelets?. Julia M. Burkhart, Stepan Gambaryan, Stephen P. Watson, Kerstin Jurk, Ulrich ... What Can Proteomics Tell Us About Platelets?. Julia M. Burkhart, Stepan Gambaryan, Stephen P. Watson, Kerstin Jurk, Ulrich ... What Can Proteomics Tell Us About Platelets?. Julia M. Burkhart, Stepan Gambaryan, Stephen P. Watson, Kerstin Jurk, Ulrich
http://circres.ahajournals.org/content/114/7/1204.figures-only
*  Biovital Research - Proteomics, Bioimaging, Bioanalysis
Biovital Research exists to deliver innovative solutions to the plant science community in the biomarker and environmental testing fields, as well as providing
http://biovital.org.uk
*  01:694:412 Proteomics and Functional Genomics
01:694:412 Proteomics and Functional Genomics Wait List Sign Up for Spring 2017 Courses ... This course is a survey of modern techniques of protein biochemistry, bioinformatics, proteomics, and functional genomics. It ... should demonstrate an understanding of the knowledge that is needed to begin biomedical research in the areas of proteomics, ...
http://biology.rutgers.edu/biological-sciences/courses/31-spring-courses/molecular-biology-and-biochemistry-courses-2/206-694-412-proteomics-and-functional-genomics
*  sepCV | HUPO Proteomics Standards Initiative
sepCV. HUPO Proteomics Standards Initiative. Skip to main content. Main menu. Search. Home. About. Groups. Mass Spectrometry. Protein Modifications. Protein Separation. Proteomics Informatics. Sample Processing. Controlled vocabularies. MIAPE. PSI Editors. Steering Committee. Specifications. TraML. GelML. MIF. mzIdentML. mzML. mzQuantML. mzTab. MIAPE. Controlled vocabularies. Discuss. Users. Events. HUPO-PSI 2015. HUPO-PSI 2014. HUPO-PSI 2013. Publications. HUPO Proteomics Standards Initiative. sepCV. sepCV: Sample processing and separations controlled vocabulary. . Motivation. The sepCV is a controlled vocabulary for teminology associated with sample preparation and sample processing for proteomics. The sepCV was originally designed to provide terminology for the MIAPE GE reporting guidelines and the gelML data transfer format. It has now been expanded to include terminology for gel image informatics to support the MIAPE GI reporting guidelines and the GelInfoML data transfer ...
http://psidev.info/sepcv
*  Proteomics Standards Initiative
... the proteomics standards initiative psi is a working group of human proteome organization it aims to define data standards for proteomics in order to facilitate data comparison exchange and verification the proteomics standards initiati focuses on the following subjects minimum information about a proteomics experiment defines the metadata that should be provided along with a proteomics experiment data markup language for encoding the data and metadata ontologies for consistent annotation and representation references category bioinformatics software
https://en.wikipedia.org/wiki/Proteomics_Standards_Initiative
*  Documents | HUPO Proteomics Standards Initiative
Documents. HUPO Proteomics Standards Initiative. Skip to main content Main menu Search. Home. About. Groups. Mass Spectrometry. Molecular Interactions. Protein Modifications. Protein Separation. Proteomics Informatics. Sample Processing. Controlled vocabularies. MIAPE. PSI Editors. Steering Committee. Specifications. TraML. GelML. MIF. mzIdentML. mzML. mzQuantML. mzTab. MIAPE. Controlled vocabularies. Discuss. Users. Events. HUPO-PSI 2015. HUPO-PSI 2014. HUPO-PSI 2013. Publications. HUPO Proteomics Standards Initiative. You are here Groups. Sample Processing. Documents spML Milestone 2 Documents Posted Thu, 01/26/2012 - 04:19. Sample Processing Workgroup Posted Tue, 01/24/2012 - 19:09. The Proteomics Standards Initiative PSI is a part of the. Human Proteome Organisation. HUPO.
http://psidev.info/groups/sample-processing/documents
*  Mass Spectrometry Workgroup | HUPO Proteomics Standards Initiative
Mass Spectrometry Workgroup. HUPO Proteomics Standards Initiative. Mass Spectrometry. Proteomics Informatics. HUPO-PSI 2015. HUPO-PSI 2014. HUPO-PSI 2013. HUPO Proteomics Standards Initiative. Mass Spectrometry Workgroup PSI-MS: Mass Spectrometry Standards Working Group About us. The mzML format. Controlled vocabulary development. Email discussion list. About us The PSI-MSS working group defines community data formats and controlled vocabulary terms facilitating data exchange and archiving in the the field of proteomics mass spectrometry. Current projects are: The mzML format, which merges the mzData format see below and another similar format mzXML. See the TraML information page for the full specification, other documentation and examples. mzData's aim is to unite the large number of current formats pkl's, dta's, mgf's, ..... Wilfred Tang, Applied Biosystems Pete Souda, University of California, Los Angeles Angel Pizarro, University of Pennsylvania Sean Seymour, Applied ...
http://psidev.info/mass-spectrometry
*  Sample Processing Workgroup | HUPO Proteomics Standards Initiative
Sample Processing Workgroup. HUPO Proteomics Standards Initiative. MIAPE. MIAPE. HUPO-PSI 2015. HUPO-PSI 2014. HUPO-PSI 2013. HUPO Proteomics Standards Initiative. Sample Processing Workgroup The Sample Processing Working Group has been merged with the gel group into a. Controlled vocabulary development. Statement of purpose The remit of the Sample Processing Working Group is to produce reporting guidelines, data exchange formats and controlled vocabulary covering all separation techniques not considered to be 'classical' one- or two-dimensional gel electrophoresis cf. Minimum reporting requirements The evolving Minimum Information About a Proteomics Experiment MIAPE documents offer guidelines on how to adequately report a proteomics experiment. XML formats for data exchange Derived from the FuGE general object model, the formats developed by this workgroup are designed to function both as standalone files and as part of a 'parent' FuGE-ML document. These formats will facilitate ...
http://psidev.info/index.php?q=node/31
*  Proteomics Journals | Peer Reviewed | High Impact Articles
Related Journals of Microarray Proteomics Journal of Data Mining in Genomics Proteomics, Transcriptomics: Open Access, Journal of Next Generation Sequencing Applications, Journal of Proteome Research, Journal of Proteomics, Molecular and Cellular Proteomics, Journal of Proteome Science and Computational Biology, International Journal of Proteomics, Proteome Science, Clinical Proteomics, Current Proteomics Proteomic Analysis Proteomic analysis is the complete identification and quantification of the proteome i.e. Related Journals of Proteomics Analysis Mass Spectrometry: Open Access, Metabolomics:Open Access, Journal of Data Mining in Genomics Proteomics, Translational proteomics, Internet Journal of Genomics and Proteomics, Proteomics Clinical applications, Cancer Biomarkers, Open Biomarkers Journal, Cancer Epidemiology Biomarkers and Prevention, Epigenomics, Human Genomics and Proteomics Proteomics Clinical ...
http://omicsonline.org/proteomics-bioinformatics.php
*  Human Proteome Organization
... the human proteome organization hupo is an international consortium of national proteomics research associations government researchers academic institutions and industry partners the organization was founded in june and it promotes the development and awareness of proteomics research advocates on behalf of proteomics researchers throughout the world and facilitates scientific collaborations between members and initiatives ultimately it is organized to gain a better and more complete understanding of the human proteome key members past and present see also references external links key members past and present rolf apweiler samir hanash matthias mann gil omenn young ki paik see also human genome organisation human genome project proteomics standards initiative human proteome project references external links category research projects
https://en.wikipedia.org/wiki/Human_Proteome_Organization
*  Shotgun proteomics
... 'Shotgun proteomics ' refers to the use of bottom-up proteomics techniques in identifying protein s in complex mixtures using a combination of high performance liquid chromatography combined with mass spectrometry. The most common method of shotgun proteomics starts with the proteins in the mixture being digested and the resulting peptide s are separated by liquid chromatography. Tandem mass spectrometry is then used to identify the peptides. History Advantages Disadvantages Workflow Issues with peptide identification Practical Applications Large-scale analysis. Shotgun proteomics allows global protein identification as well as the ability to systematically profile dynamic proteomes. The dynamic exclusion filtering that is often used in shotgun proteomics maximizes the number of identified proteins at the expense of random sampling. thumb|right|300 px|Agilent 1200 HPLC |thumb|right|300 px|Quadrupole Time-Of-Flight tandem Mass Spectrometer Q-TOF. As the peptides elute ...
https://en.wikipedia.org/wiki/Shotgun_proteomics
*  The application of 2D gel-based proteomics methods to the study of breast cancer. - UCL Discovery
... UCL Discovery. UCL home » Library Services » Electronic resources » UCL Discovery Enter your search terms. Advanced search. UCL Theses. For everyone Open Access. About UCL Discovery. For UCL authors Deposit your research. UCL Publications Policy. How to deposit. UCL e-theses guidelines. The application of 2D gel-based proteomics methods to the study of breast cancer. The application of 2D gel-based proteomics methods to the study of breast cancer. Abstract The protein complement of breast cells consists of many thousands of proteins. Recent developments in 2D gel electrophoresis technology have made studies requiring the quantitative analysis of a differential proteome, such as comparison between normal and malignant cells or investigation of drug effects on cells, truly feasible. In this paper, the technology and limitations of 2D gel-based proteomics are reviewed. Techniques for comparing sets of gels at a global level as well as identifying specific protein features that ...
http://discovery.ucl.ac.uk/185529/
*  Minimum information about a proteomics experiment
... 'Minimum information about a proteomics experiment' 'MIAPE' is a minimum information standard, created by the Proteomics Standards Initiative of the Human Proteome Organization, for reporting proteomics experiments. 3 Working groups Gel electrophoresis. Standard-compliant proteomics repositories Similar initiatives References. The gel electrophoresis working group defined reporting requirements for gel electrophoresis experiments. 6 The gel electrophoresis working group also focuses on image analysis with the gel image informatics recommendation that is currently in public review phase while the corresponding exchange format is only a draft as of April 2009. The sample processing working group defines requirements concerning all the sample pre-processing steps that are carried out before gel electrophoresis or mass spectrometry is applied. 8 and mass spectrometry informatics. 9 documents have been published as recommendations by the mass spectrometry working group. The ...
https://en.wikipedia.org/wiki/Minimum_information_about_a_proteomics_experiment
*  2015 | Institute for Systems Biology
Institute for Systems Biology. What Is Systems Biology. Baliga Lab. Huang Lab. Price Lab. Center for Systems Biology. C., Djukovic, D., Gu, H., Raftery, D., Price, N. Genes, Brain, and Behavior. L., Shteynberg, D., Hood, L., Moritz, R. N., Moritz, R. http://doi.org/10.1021/pr501321h. http://doi.org/10.1021/ac504110t. http://doi.org/10.1016/j.pbiomolbio.2015.06.003. Cell, 162, 18 20. http://doi.org/10.1016/j.cell.2015.06.041. http://doi.org/10.1038/srep08638. A comprehensive map of genome-wide gene regulation in Mycobacterium tuberculosis. Cancer cell-autonomous TRAIL-R signaling promotes KRAS-driven cancer progression, invasion, and metastasis. cMonkey2: Automated, systematic, integrated detection of co-regulated gene modules for any organism. Data-driven integration of genome-scale regulatory and metabolic network models. Development of data representation standards by the human proteome organization proteomics standards initiative. Lee, M.-J., Chen, Y.-F., Fan, P.-C., Wang, K.-C., Wang, K., Wang, ...
https://systemsbiology.org/publications/2015-2/
*  Proteomics
'Proteomics' is the large-scale study of protein s, particularly their structures and functions. Complexity of the problem Post-translational modifications. Limitations of genomics and proteomics studies Methods of studying proteins Protein detection with antibodies immunoassays. Structural proteomics Expression proteomics Interaction proteomics Current proteomic technologies Mass spectrometry and protein profiling. Bioinformatics for proteomics proteome informatics Protein identification. Post-translational modifications. Computational methods in studying protein biomarkers. Emerging trends in proteomics Human plasma proteome. Post-translational modifications. Many of these post-translational modifications are critical to the protein's function. Methods such as phosphoproteomics and glycoproteomics are used to study post-translational modifications. Methods of studying proteins. In proteomics, there are multiple methods to study proteins. ...
https://en.wikipedia.org/wiki/Proteomics
*  PROTEOMICS - Clinical Applications - Volume 9, Issue 7-8 - Focus on Proteomics in Pharmaceutical Res
... earch and Development - Wiley Online Library. PROTEOMICS - Clinical Applications. Vol 9 Issue 7-8. Journal Home. FIND ISSUES. All Issues. Virtual Issues. FIND ARTICLES. Accepted Articles. Reviews. GET ACCESS. Submit an Article. OnlineOpen. ABOUT THIS JOURNAL. Contact. Editorial Board. Special Issues - Archive. Viewpoint Forum. Wiley Job Network. PROTEOMICS - Clinical Applications. Special Issue: Focus on Proteomics in Pharmaceutical Research and Development. Volume 9, Issue 7-8. Issue edited by: Paul Cutler, Hans Voshol. Cover Picture. Top of page. Cover Picture. Editorial Board. Contents. Editorial. Reviews. Viewpoint. Reviews. Addendum. You have free access to this content. Cover Picture: Proteomics 7-8'15 Article first published online: 14 JUL 2015. DOI: 10.1002/prca.201570040. Abstract. PDF 1636K. PDF 1636K. Request Permissions. Editorial Board. Top of page. Cover Picture. Editorial Board. Contents. Editorial. Reviews. Viewpoint. Reviews. Addendum. Editorial board: ...
http://onlinelibrary.wiley.com/doi/10.1002/prca.v9.7-8/issuetoc
*  Translational Proteomics - Journal - Elsevier
... Books Journals. Translational Proteomics. Translational Proteomics Editor in Chief: Jean-Charles Sanchez, PhD View full editorial board Open Access. Guide for Authors Author instructions Download the ‘Author Information Pack’ PDF. View ‘Guide for Authors’ online Useful links Read the ‘8 Reasons Why I Accepted Your Article’ blog. Download the ‘Understanding the Publishing Process’ PDF. Submit Your Paper Track Your Paper View Articles. Translational Proteomics is a HUPO-affiliated journal. Translational Proteomics covers all areas of human proteomics using multi-disciplinary approaches to untangle complex disease processes. Emphasis is placed on linking basic sciences to clinical research from patient to bench to bedside. Clinical Proteomics and Personalized medicine. Translational Proteomics is intended to academic, industrial and clinical researchers, physicians, pharmaceutical scientists, biochemists, clinical chemists, disease molecular biologists in ...
http://journals.elsevier.com/translational-proteomics
*  Proteomic Analyses of Soybean Root Tips During Germination | BenthamScience
Proteomic Analyses of Soybean Root Tips During Germination. Keywords: Gel-free proteomics, germination, root tip, SDS-gel electrophoresis-based proteomics, soybean, two-dimensional gel electrophoresis-based proteomics. Proteins were extracted from the root tips of 4-dayold soybean seedlings and analyzed using two-dimensional 2D gel electrophoresis-based proteomics, SDS-gel based proteomics, and gel-free proteomics techniques. A total of 121, 862, and 341 proteins were identified in root tips using the 2D gel-based, SDS gel-based, and gel-free proteomic techniques, respectively. The proteins identified by 2D gel-based proteomic analysis were predominantly localized in the cytoplasm, whereas nuclear-localized proteins were most commonly identified by the SDS gel-based and gel-free proteomics techniques. Of the 862 proteins identified in the SDS gelbased proteomic analysis, 190 were protein synthesis-related proteins. Taken together, these results suggest that a ...
http://eurekaselect.com/122400
*  .. Evotec, Dow AgroSciences Team Up in Chemical Proteomics Effort ..
send to printer gen news highlights jul evotec dow agrosciences team up in chemical proteomics effort evotec and dow agrosciences are collaborating in an effort to support compounds in development at the latter firm under the terms of the research agreement dow will leverage evotec s chemical proteomics services evotec will be performing quantitative chemical proteomics services to clear up dow s phenotypic screening results financial terms were not disclosed we look forward to working with dow agrosciences on this project evotec coo mario polywka ph d said in a statement this collaboration highlights the broad applicability of evotec s industry leading chemical proteomics platform to determine cellular target affinities and mechanisms of action on a proteome wide level and in a native context added dow s william kleschick ph d discovery global leader this collaboration enhances technology toolbox to accelerate our efforts to deliver novel solutions to our customers around the ...
http://genengnews.com/keywordsandtools/print/4/31903/
*  Analysis of Phosphoinositides and their Interactions > Yale/NIDA Neuroproteomics Center | W.M. Keck
Analysis of Phosphoinositides and their Interactions > Yale/NIDA Neuroproteomics Center. Keck Foundation. Yale School of Medicine. Yale School of Medicine W.M. Keck Foundation. Yale/NIDA Neuroproteomics Center. Publications. Pilot Grants. Yale/NIDA Neuroproteomics Center Publications. Yale/NIDA Neuroproteomics Center Poster Abstracts. Pilot Grants. Publications. Yale/NIDA Neuroproteomics Center Publications. Yale/NIDA Neuroproteomics Center Poster Abstracts. Analysis of Phosphoinositides and their Interactions Assays Available for the Analysis of Phosphoinositides and their Interactions Phosphoinositides PIPs, the phosphorylated metabolites of phosphatidylinositol, are membrane phospholipids that play critical roles in cell regulation. Heterogeneous distribution of PIPs in intracellular membranes. PIPs are interconverted by specific kinases and phosphatases Quantitation of Phosphoinositide levels: HPLC analysis of anionic lipid extracts from tissues and/or organs to ...
http://medicine.yale.edu/keck/nida/Phosphoinositides/Phosphoinositides.aspx
*  Wiley: PROTEOMICS -
United States Change My Account Cart Home Subjects About Wiley Contact Us Help Search Form Search Input Print this page Share Home / Life Sciences / Cell & Molecular Biology / General Cell & Molecular Biology Journal PROTEOMICS Vol 15 (22 Issues in 2015) Edited by: Michael J. Dunn Print ISSN: 1615-9853 Online ISSN: 1615-9861 Published on behalf of Human Proteome Organisation Impact Factor: 3.807 Read Now Online Description PROTEOMICS is the premier international source for information on all aspects of applications and technologies in proteomics. It provides the most comprehensive coverage in the field, spanning significant technical developments in all major gel and non-gel based proteomic platforms and their application in every area of life sciences. Papers describing novel applications of proteomics are especially welcome. Topics include whole proteome analysis of any organism, expression profiling, disease studies, pharmaceutical, agricultural and biotechnological ...
http://wiley.com/WileyCDA/WileyTitle/productCd-2120.html
*  Director of Proteomics at the Broad Institute of MIT and Harvard, Steven Carr, to Deliver Pittcon 20
... 14 Wallace H. Coulter Plenary Lecture. Science. Director of Proteomics at the Broad Institute of MIT and Harvard, Steven Carr, to Deliver Pittcon 2014 Wallace H. Coulter Plenary Lecture. The Pittcon Program Committee is pleased to announce that Steven Carr, director of proteomics at the Broad Institute of MIT and Harvard, will deliver the Wallace H. Coulter Plenary Lecture at PIttcon 2014. Carr. Significant improvements in mass spectrometry-based proteomic technologies over the past few years have greatly increased the value and necessity of analyzing proteins and their modifications in biology and medicine. Pittsburgh, PA PRWEB May 15, 2013 Dr. Carr is internationally recognized as a leader in the development of novel proteomics methods and in their application in biology and medicine. Carr and his group collaborate with scientists throughout the greater Broad community Broad Institute, Harvard, Harvard Medical School, and the 17 Harvard affiliated hospitals to apply state-of-the ...
http://prweb.com/releases/prweb2013/5/prweb10727233.htm
*  Functional Proteomics - Mukhopadhyay Lab - UT Southwestern
... Research Research Background Signaling Pathways I Signaling Pathways II Cilia & Development Functional Proteomics Ciliary Diseases Ciliary Signaling & Cancer. Research Background Signaling Pathways I Signaling Pathways II Cilia & Development Functional Proteomics Ciliary Diseases Ciliary Signaling & Cancer. Pioneering studies in yeast describe genome-wide proteomic networks by tandem affinity purification TAP purification and mass-spectrometry MS -based methods Gavin et al., 2002. Description of our general strategy for building functional proteomic networks TAP-MS-based protein-protein interaction studies provide rapid insights into the function of a protein in the pertinent cellular context. In parallel, we also subject the tandem purification eluates to biochemical and functional studies, and verify reciprocal interactions by further TAP-MS-based proteomics of the protein interactors. A complementary proteomic strategy to solve complex biological problems This general framework ...
http://utsouthwestern.edu/labs/mukhopadhyay/research/functional-proteomics.html
*  Quantitative proteomics using uniform N-15-labeling, MASCOT, and the trans-proteomic pipeline - Ce
... ntAUR. . University of Reading CentAUR: Central Archive at the University of Reading. Accessibility navigation Quantitative proteomics using uniform N-15-labeling, MASCOT, and the trans-proteomic pipeline. Tools Tools Tools. Wordle Link RDF+XML BibTeX RIOXX2 XML RDF+N-Triples JSON Dublin Core Atom Simple Metadata Refer METS HTML Citation with IDs HTML Citation ASCII Citation OpenURL ContextObject EndNote OpenURL ContextObject in Span MODS MPEG-21 DIDL EP3 XML Dublin Core Reference Manager RDF+N3. Palmblad, M., Bindschedler, L.V. and. Cramer, R. 2007 Quantitative proteomics using uniform N-15-labeling, MASCOT, and the trans-proteomic pipeline. Proteomics, 7 19. pp. 3462-3469. ISSN 1615-9853 Full text not archived in this repository. To link to this article DOI: 10.1002/pmic.200700180 Abstract/Summary Stable isotope labeling combined with MS is a powerful method for measuring relative protein abundances, for instance, by differential metabolic labeling of some or all amino acids with ...
http://centaur.reading.ac.uk/11545/
*  Proteomics/Mass Spectrometry Laboratory (P/MSL)
Proteomics/Mass Spectrometry Laboratory P/MSL. QB3 Central Website Vincent J. Coates Proteomics/Mass Spectrometry Laboratory. QB3-Berkeley. Home. Samples. Protocols. Sample Preparation Tips. Sample Submission Form. Rates. Services. Identification of Proteins in Complex Mixtures. Identification of Protein Modifications. Identification of Proteins from Gel Bands. Relative Quantitation in Complex Mixtures. Contacts. Staff Contacts. Ready to Publish. Resources. Vincent J. Coates Proteomics/Mass Spectrometry Laboratory P/MSL The UCB Proteomics/Mass Spectrometry Laboratory P/MSL was founded in 2004 as part of UCB s Cancer Research Laboratory. In 2007, we became the Vincent J. Coates Proteomics Laboratory, affiliated with QB3, in order to provide improved access to Proteomics services. We now occupy a spacious laboratory in Stanley Hall with a wet biochemistry area for protein sample preparation and room for expansion of our array of mass spectrometers. The P/MSL provides ...
http://qb3.berkeley.edu/qb3/pmsl/index.cfm
*  Quantitative proteomic analysis of ibuprofen-degrading Patulibacter sp. strain I11 - Forskningsporta
... l, Aalborg Universitet. Om AAU. Quantitative proteomic analysis of ibuprofen-degra... Quantitative proteomic analysis of ibuprofen-degrading Patulibacter sp. strain I11 Publikation : Forskning - peer review › Tidsskriftartikel. Several isolates have been shown to degrade ibuprofen, but very little is known about the biochemistry of this process. The bacterial proteomes of actively ibuprofen-degrading cells and cells grown in the absence of ibuprofen was identified and quantified by gel based shotgun-proteomics. Biological process and pathway analysis indicated a number of proteins that were up-regulated in response to active degradation of ibuprofen, some of them are known to be involved in the degradation of aromatic compounds. Data analysis revealed that several of these proteins are likely involved in ibuprofen degradation by Patulibacter sp. Several isolates have been shown to degrade ibuprofen, but very little is known about the biochemistry of this process. The bacterial proteomes of ...
http://vbn.aau.dk/da/publications/quantitative-proteomic-analysis-of-ibuprofendegrading-patulibacter-sp-strain-i11(d41808e7-0f0a-4afb-83c0-5daee6137434).html
*  Proteomics/Introduction to Proteomics/Bioinformatics - Wikibooks, open books for an open world
... Proteomics/Introduction to Proteomics/Bioinformatics From Wikibooks, open books for an open world Proteomics. Introduction to Proteomics This page may need to be reviewed for quality. Jump to: navigation, search. What is Proteomics. Principles of Proteomics Bioinformatics in Proteomics Links. Contents. 1 Bioinformatics in Proteomics. Bioinformatics in Proteomics. A cabinet from the IBM Blue Gene L supercomputer. As with other subsets of biology, an increased ability to generate large amounts of data from the use of high throughput methods has led to an increased reliance on computers for data acquisition, storage, and analysis. The internet has also enabled collaboration and sharing of data that would have previously not been possible, leading to the development of large public databases with contributors all over the world. Many databases exist for protein-related information, such as the Protein Data Bank PDB which handles structure and ...
https://en.wikibooks.org/wiki/Proteomics/Introduction_to_Proteomics/Bioinformatics
*  Streptococcus pyogenes in human plasma: Adaptive mechanisms analyzed by mass spectrometry-based prot
Streptococcus pyogenes in human plasma: Adaptive mechanisms analyzed by mass spectrometry-based proteomics. Article Streptococcus pyogenes in human plasma: Adaptive mechanisms analyzed by mass spectrometry-based proteomics. Johan Malmström. Johan Malmström. Christofer Karlsson. Christofer Karlsson Lund University Message author. Impact Factor: 4.57. A combination of label-free quantitative mass spectrometry-based proteomics strategies were used to measure how the intracellular proteome homeostasis of S. S pyogenes expresses HSA-binding surface proteins, and HSA carrying fatty acids reduced the amount of fatty acid biosynthesis proteins to the same extent as plasma. DOI:10.1186/1471-2105-12-468 2.58 Impact Factor. Automated Workflow for Large-Scale Selected Reaction Monitoring Experiments. Johan Malmström. ABSTRACT: Targeted proteomics allows researchers to study proteins of interest without being drowned in data from other, less interesting proteins or from redundant or ...
http://researchgate.net/publication/51830359_Streptococcus_pyogenes_in_human_plasma_Adaptive_mechanisms_analyzed_by_mass_spectrometry-based_proteomics
*  Australasian Proteomics Society
... The 'Australasian Proteomics Society' APS is a learned society formed in 2004 from the Lorne Proteomics Symposia LPS meetings. The APS was expanded to include the Australian Electrophoresis and Proteomics Society a society formed in 1994 in Sydney, Australia and originally called the Australian Electrophoresis Society, part of the International Council of Electrophoresis Societies. The Lorne Proteomics Symposia meetings can trace their roots to the original Specialist Protein Analysis Workshop SPAW founded by Robert L. Moritz and Richard J. Simpson. These meetings began in 1994 as an offshoot of the Lorne Protein Meeting. established in 1974 to address the needs of protein purification and analysis technologies rapidly under development in the 1980s. The founding president and vice president were Simpson and Moritz. The current president is Stuart Cordwell. In 2004, an Australasia-wide committee was formed to fully disseminate the activities of the APS and provide a regional ...
https://en.wikipedia.org/wiki/Australasian_Proteomics_Society
*  Neuroproteomics
So far, only small subsets of the neuronal proteome have been mapped, and then only when applied to the proteins involved in the synapse. Brain Injury. Compared to simple 2D page, liquid chromatography mass spectrometry can handle a larger range of protein species size, but it is limited in the amount of protein sample it handle at once. Liquid chromatography mass spectrometry is also limited in its lack of a reference map from which to work with. Neuroproteomics is being applied to study the effect of drug addiction across the synapse. Different stages of drug abuse must be studied, however, in order to map out the progression of protein changes along the course of the drug addiction. For drug addiction, the synapse is the most likely target as it involves communication between neurons. Lack of sensory communication in neurons is often an outward sign of drug abuse, and so neuroproteomics is being applied to find out what proteins are being affected to prevent the transport of ...
https://en.wikipedia.org/wiki/Neuroproteomics
*  PLOS Collections: Computational Mass Spectrometry–Based Proteomics
... View. Sum of PLOS and PubMed Central page views and downloads. Computational Mass Spectrometry–Based Proteomics. Article. Citation: Käll L, Vitek O 2011 Computational Mass Spectrometry–Based Proteomics. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. PLoS Computational Biology. Global Label-Free LC-MS/MS Workflow. Although many alternatives exist, shotgun liquid chromatography coupled with tandem mass spectrometry LC-MS/MS; overview in Figure 3 is most frequently used. b Global label-free workflows achieve relative quantification by comparing counts of MS/MS spectra, or intensities of MS peaks between runs. This has received much attention from both algorithmic and statistical viewpoints. , Representative methods for controlling FDR are two-group models, which view the reported PSMs as a mixture of ...
http://journals.plos.org/ploscollections/article?id=10.1371/journal.pcbi.1002277
*  Bottom-up proteomics
bottom up proteomics bottom up proteomics right px thumb bottom up versus top down proteomics bottom up proteomics is a common method to identify proteins and characterize their amino acid sequences and post translational modifications by proteolytic digestion of proteins prior to analysis by mass spectrometry the proteins may first be purified by a method such as gel electrophoresis resulting in one or a few proteins in each proteolytic digest alternatively the crude protein extract is digested directly followed by one or more dimensions of separation of the peptides by liquid chromatography coupled to mass spectrometry a technique known as shotgun proteomics by comparing the masses of the proteolytic peptides or their tandem mass spectra with those predicted from a sequence database or annotated peptide spectral in a peptide spectral library peptides can be identified and multiple peptide identifications assembled into a protein identification advantages disadvantages see ...
https://en.wikipedia.org/wiki/Bottom-up_proteomics
*  UC Davis Health System Department of Pathology: Clinical Proteomics Core Lab - Services
... UC Davis Health System. UC Davis. Department of Pathology and Laboratory Medicine. Health System. Pathology and Laboratory Medicine. Clinical Proteomics Core Lab. Department of Pathology and Laboratory Medicine Medical Diagnostics. Clinical Organizational Structure. Administrative Services. Clinical Laboratory. Clinical Services. Clinical Services. Laboratory Test Directory. Education Programs. Education Programs. Department of Pathology Courses. Laboratory Test Directory. Proteomics. Clinical Staff. Administrative Staff. Map Directions. Map Directions. UC Davis Medical Center. Contact Us. Proteomics Core Lab. Services. Contact us. Clinical Proteomics Core Lab: Services. Custom assay development:. Development of new multiplex assays to measure more than one analytes in one sample simultaneously. The client will provide basic information by completing a Multiplex Project-sheet project title, principal investigator and contact information, brief statement of project goals, ...
http://ucdmc.ucdavis.edu/pathology/proteomics/services.html
*  Metaproteomics
... 'Metaproteomics' also 'Community Proteomics', 'Environmental Proteomics', or 'Community Proteogenomics' is the study of all protein samples recovered directly from environmental sources. Metaproteomics is used to classify experiments that deal with all the genes and proteins identified from complex communities, where individuals cannot be binned into species or organisms types. The metaproteomics approach is comparable to gene-centric environmental genomics, or metagenomics. Origin of the term Proteomics of microbial community See also References. Origin of the term. The term "metaproteomics" was proposed by Francisco Rodríguez-Valera to describe the genes and/or proteins most abundantly expressed in environmental samples. Environmental genomics, the big picture. FEMS Microbiol. Wilmes and Bond proposed the term "metaproteomics" for the large-scale characterization of the entire protein complement of environmental microbiota at a given point in ...
https://en.wikipedia.org/wiki/Metaproteomics
*  Proteomics - Home
Welcome to the chair of Proteomics and Bioanalytics. The chair of Proteomics and Bioanalytics at the Technische Universit t M nchen comprises an international research team with a focus on proteomics, chemical biology and biomarker discovery. Most of our research builds on the powerful combination of affinity chromatography and quantitative mass spectrometry along with sophisticated bioinformatics tools. We develop novel analytical methods for quantitative proteome research notably for high throughput applications, the analysis of post translational protein modifications and protein interactions. In our chemical biology efforts, we elucidate the selectivity of drug-target interactions on a proteome-wide scale, investigate the cellular mechanisms by which drugs exert their function and identify mechanisms of cancer therapy resistance. The biomarker discovery projects focus on cancer biology and include the identification of phosphoproteomic signaling biomarkers for drug and patient ...
http://wzw.tum.de/proteomics/
*  Proteogenomics
... 'Proteogenomics' is an emerging field of biological research at the intersection of proteomics and genomics. While this intersection is large and can be defined in multiple ways, the term proteogenomics commonly refers to studies that use proteomic information, often derived from mass spectrometry, to improve gene annotations. D 2008 Proteogenomics: needs and roles to be filled by proteomics in genome annotation. Practical applications Methodology References External links. Practical applications. Proteogenomics has been applied to improve the gene annotations of various organisms. 1996 Linking genome and proteome by mass spectrometry: large-scale identification of yeast proteins from two dimensional gels. H., Shen Z., Stanke M., Bafna V., Briggs S. humans, Tanner S., Shen Z., Ng J., Florea L., Guigo R., Briggs S.P., Bafna V. Improving gene annotation using peptide mass spectrometry. Genome Res. multiple species of ' Shewanella ' bacteria, Gupta N., Tanner S., Jaitly N., Adkins J.N., ...
https://en.wikipedia.org/wiki/Proteogenomics
*  Proteomics/Proteolytic Processing - Wikibooks, open books for an open world
... Open main menu. Proteomics/Proteolytic Processing. Proteomics This page may need to be reviewed for quality. Previous chapter: Proteomics and Drug Discovery. Introduction Proteomics/Proteolytic processing/Proprotein Proteomics/Proteolytic processing/Specificity of proteolytic processing Proteomics/Proteolytic processing/Proteolytic processing in Secretory Pathway Proteomics/Proteolytic processing/Examples of proteolytic processing in various organs of our body Proteomics/Proteolytic processing/Proteolytic processing of the Human Immunodeficiency Virus type 1 Proteomics/Proteolytic processing/Proteolytic processing- role in therapeutics Proteomics/Proteolytic processing/Autoprocessing Proteomics/Proteolytic processing/Lethal effects of proteolytic processing. Last edited on 20 July 2009, at 09:34. Wikibooks ®. Mobile Desktop Content is available under CC BY-SA 3.0 unless otherwise noted. Terms of Use. Privacy.
https://en.m.wikibooks.org/wiki/Proteomics/Proteolytic_Processing
*  Identification of β-Secretase (BACE1) Substrates Using Quantitative Proteomics
Identification of β-Secretase BACE1 Substrates Using Quantitative Proteomics. Identification of β-Secretase BACE1 Substrates Using Quantitative Proteomics. DASH Home. HMS Scholarly Articles View Item. Identification of β-Secretase BACE1 Substrates Using Quantitative Proteomics. Download Full Text. Identification of β-Secretase BACE1 Substrates Using Quantitative Proteomics. Elias, Joshua E. ; Selkoe, Dennis J. ; Hemming, Matthew Louis Note: Order does not necessarily reflect citation order of authors. Hemming, Matthew L., Joshua E. Elias, Steven P. Gygi, and Dennis J. Identification of β-Secretase BACE1 substrates using quantitative proteomics. Full Text Related Files:. 2793532.pdf 1.329Mb; PDF. However, the substrates that BACE1 processes to regulate these functions are unknown, and to date only a few β-secretase substrates have been identified through candidate-based studies. Using an unbiased approach to substrate identification, we performed quantitative proteomic ...
http://dash.harvard.edu/handle/1/8296053
*  Proteomics Center Tools | proteomics.mcw.edu
proteomics center tools proteomics mcw edu home contact us proteomics tools pepid denovoid promost zoomquant visualize epitomize vipdac related links bioinformatics hmgc home bbc home mcw home nhlbi home nhlbi proteomics home welcome to the mcw proteomics tools these tools were developed as part of a national heart lung and blood institute nhlbi proteomics center project to aid proteomics researchers in proteomics data visualization and analysis this page contains both web based tools used to identify post translationally modified proteins on d gels and peptides based on their composition as well as downloadable tools to analyze o data and visualize quantitate and analyze high throughput proteomic data sets bioinformatics program hmgc medical college of wisconsin this project is funded by the national heart lung and blood institute
http://proteomics.mcw.edu/
*  Activity-based proteomics
... Image:Fp rhodamine.svg Image:Gel-abpp eg.png|frame|left|In- gel ABPP using probes with different fluorophores in the same lane to simultaneously profile differences in enzyme activities. 'Activity-based proteomics', or 'activity-based protein profiling' 'ABPP' is a functional proteomic technology that uses chemical probes that react with mechanistically related classes of enzymes. 'Activity-based protein profiling: applications to biomarker discovery, in vivo imaging and drug discovery.' American Journal of Pharmacogenomics 2004 The basic unit of ABPP is the probe, which typically consists of two elements: a reactive group RG, sometimes called a "warhead" and a tag. 'Activity-Based Protein Profiling in Vivo Using a Copper I -Cataqlyzed Azide-Alkyne Cycloaddition' Journal of the American Chemical Society 2003. 'Activity-based protein profiling: The serine hydrolases' Proceedings of the National Academy of Sciences 1999 and metalloprotease s Saghatelian A, et al. 'Activity-based probes for the ...
https://en.wikipedia.org/wiki/Activity-based_proteomics
*  Quantitative proteomics
... is mainly performed by two-dimensional gel electrophoresis 2-DE or mass spectrometry MS. Technologies Discovery and targeted proteomics Relative and absolute quantitation Label-free quantification. Stable isotope labels. For quantitative MS, a commonly applied approach is isotope-coded affinity tag s ICAT, which uses two reagents with heavy and light isotopes, respectively, and a biotin affinity tag to modify cysteine containing peptides. Relative and absolute quantitation. Mass spectrometry is not inherently quantitative because of differences in the ionization efficiency and/or detectability of the many peptides in a given sample, which has sparked the development of methods to determine relative and absolute abundance of proteins in samples. One approach for relative quantitation is to separately analyze samples by MS and compare the spectra to determine peptide abundance in one sample relative to another, as in Label-free quantification strategies. Stable isotope labels. An approach for ...
https://en.wikipedia.org/wiki/Quantitative_proteomics
*  new proteomics publication
... Dima Klenchin klenchin at REMOVE TO REPLY.facstaff.wisc.edu. Thu Jul 5 19:11:50 EST 2001. Previous message: new proteomics publication Next message: new proteomics publication Messages sorted by:. Dr. Artem Evdokimov eudokima at mail.ncifcrf.gov wrote: : gene transfer genetics : signal transduction signal transduction : apoptosis journal of apoptosis : microarrays biotechniques : gene expression biotechniques : SNP technologies same : data mining fancy name for computational biology : 2-D gel electrophoresis journal of electrophoresis : mass spectroscopy several MS journals : robotics and automation techniques : protein characterization biochemistry, protein analysis : PCR biotechniques, journal of pcr : : And so I wonder : what IS proteomics. Why is it different from good ole : biochemistry. Formally, the term proteomics was coined to ease getting more money for whatever protein-related research. Fanciful name = new = more chances to be funded. Apart from that and trying to ...
http://bio.net/bionet/mm/proteins/2001-July/009532.html
*  Proteomics Facility
... Skip to content Skip to navigation menu. Proteomics Facility Use: The Proteomics facility has recently benefitted from generous support through SRIF3. We offer state of the art facilities for the protein and peptide seperation, the identification of proteins on a global sacle, the analysis of individual proteins and the characterisation of protein-protein interactions. The facility presently offers the following resources for: 1D/2D electrophoresis GE Healthcare Ettan IPGPhor, DALT VI and multiphor systems, Invitrogen mini-gel system Gel scanning and analysis GE ImageScanner and Nonliner Dynamics Phoretix 2D Expression Software 2D fluorescence difference gel electrophoresis DIGE; GE Typhoon Trio varible mode imager and DeCyder 2D Version 6.5 software Free flow electrophoresis BD diagnostics Lab-on-a-chip microfluidics-based protein electrophoresis and quantification Agilent 2100 Bioanalyser Automated spot picking Ettan Spot Picker and in-gel trypsin digestion Ettan digester ...
http://cardiff.ac.uk/citer/research/equipment/proteomics-facility.html
*  Reportlinker Adds Proteomics: Technologies and Global Mark... (X-Ray Crystallography TABLE 71 .
Reportlinker Adds Proteomics: Technologies and Global Mark... X-Ray Crystallography TABLE 71 ... Home. Biology. Medicine. Technology. Products. News. Definition. Dictionary. Movies. Links. Tags. Search. RSS Navigation Links Biology News Medicine News Biology Products Medicine Products Biology Definition Medicine Definition Biology Technology Medicine Technology Biology Dictionary Medicine Dictionary. HOME >> BIOLOGY >> NEWS. Reportlinker Adds Proteomics: Technologies and Global Markets Report X-Ray Crystallography ... ...TABLE 71 GLOBAL X-RAY CRYSTALLOGRAPHY MARKET BY GEOGRAPHIC REGION THR... ...Market Drivers ... http://www.bio-medicine.org/inc/biomed/biology-news.asp http://feeds.bio-medicine.org/latest-biology-news Date:9/21/2009. >X-Ray Crystallography. TABLE 71 GLOBAL X-RAY CRYSTALLOGRAPHY MARKET BY GEOGRAPHIC REGION, THROUGH 2014 $ MILLIONS. Market Drivers Application in Drug Discovery Boosting Market Growth Simple and Low Cost Solution Increasing Adoption Rates Aids Effective Analysis in ...
http://bio-medicine.org/biology-news-1/Reportlinker-Adds-Proteomics-3A-Technologies-and-Global-Markets-Report-10023-13/
*  PNNL: BTRR Proteomics Resource for Integrative Biology
... Pacific Northwest National Laboratory Skip to Main Content U.S. Department of Energy. Search PNNL. PNNL Home. About. Research. Publications. Jobs. Newsroom. Contacts. Proteomics Research Resource for Integrative Biology. Welcome to the Proteomics Research Resource for Integrative Biology, established to provide new proteomic technologies for use in the biomedical research community. The possible roles and importance of proteomics are rapidly growing across essentially all areas of biological research. The precise and comprehensive measurement of levels of expressed proteins and their modified forms can provide new insights into the molecular nature of cell-signaling pathways and networks, the cell cycle, cellular differentiation, and other processes relevant to understanding human health and the progression of various disease states. The ability to characterize protein complexes complements this capability, allowing hypotheses to be tested and the biological system operation to be ...
http://panomics.pnnl.gov/
*  PerkinElmer and Vivascience develop proteomics platform
... FREE NEWSLETTER. Breaking News on Contract Research, Manufacturing & Clinical Trials. Headlines Preclinical Research Clinical Development Contract Manufacturing Commercial Services Video Audio Gallery Internet Live Events. Service Sectors Analytical testing, QC Bulk ingredients Contract Manufacturing Contract sales & marketing Data management Drug delivery, formulation Fill & finish, packaging. Related sites in-PharmaTechnologist.com Global Pharmaceutical Technology & Manufacturing. Subscribe to our FREE newsletter. Drug discovery company PerkinElmer and biotechnology company Vivascience has announced the formation of a joint partnership and distribution agreement in biomarker screening and discovery. The hope is that combining the company's proprietary technology; the rate of discovery of novel, informative biomarkers will dramatically improve the speed and efficiency of drug discovery which is one of the primary drivers of the proteomics market. Under the agreement, Vivascience will supply its ...
http://outsourcing-pharma.com/Preclinical-Research/PerkinElmer-and-Vivascience-develop-proteomics-platform
*  Mass Spectrometry/Proteomics Core Laboratory Protocol for In-Solution Trypsin Digestion
... University of Kansas Medical Center Directory. Jobs. Maps. News. Quick Find. Events Today. Featured. Your browser must support JavaScript to view this content. Please enable JavaScript in your browser settings then try again. Your browser must support JavaScript to view this content. Please enable JavaScript in your browser settings then try again. Subscribe to KUMC Events Calendar via Outlook RSS of Events. Information for: Students. Residents Faculty Staff Researchers Patients Prospective Employees Alumni. Donors Academics Blackboard. JayDocs The Beat Enroll Pay Dykes Library Academic Calendar More Student Services. Outlook myKUMC SharePoint HR/Payroll System. JayLog OASIS Secure File Transfers VPN. KUMC Leadership Facilities & Maintenance Human Resources Information Resources. KUMC A-Z:. A. B. C. D. E. F. G. H. I. J. K. L. M. N. O. P. Q. R. S. T. U. V. W. X. Y. Z. all. Graduate Studies. School of Health Professions. School of Nursing. School of Medicine. KU - Edwards. KU - Lawrence. KU SOM - Salina. ...
http://kumc.edu/mspc/protocols/in-solution-trypsin-digestion.html
*  Browsing Proteomics Center publications (MU) by Author "Emerich, David W. (David William)"
Browsing Proteomics Center publications MU by Author "Emerich, David W. David William ". Toggle navigation. Cited more. Safe forever. advanced search. submit works. about. contact us. login. Toggle navigation. Browsing Proteomics Center publications MU by Author. MOspace Home. University of Missouri-Columbia. Christopher S. Bond Life Sciences Center MU. Proteomics Center MU. Proteomics Center publications MU. Browsing Proteomics Center publications MU by Author. MOspace Home. University of Missouri-Columbia. Christopher S. Bond Life Sciences Center MU. Proteomics Center MU. Proteomics Center publications MU. Browsing Proteomics Center publications MU by Author. Search MOspace. This Collection. All of MOspace Communities Collections Date Issued Author Author/Contributor Title Subject Identifier Thesis Department Thesis Advisor Thesis Semester This Collection Date Issued Author Author/Contributor Title Subject Identifier Thesis Department Thesis Advisor ...
https://mospace.umsystem.edu/xmlui/handle/10355/3237/browse?value=Emerich, David W. (David William)&type=author
*  12th HUPO World Congress - Events on SelectScience
... Clinical. Drug Discovery. Food and Beverage. General Laboratory. Life Sciences. Spectroscopy. Products Reviews. Clinical. General Laboratory. Life Sciences. Write a Review. Visit Drug Discovery & Development Food and Beverage Latest: Optimize Your Pesticide Detection and Quantitation Visit Food and Beverage Homepage. Spectroscopy. Acoustic Liquid Handling Accelerating Drug Discovery at Cancer Research UK Manchester Institute. Evaluation of the Effect of Red Chilli Addition to Food Products on Their Shelf-Life. Circulating Cell-Free DNA and Circulating Tumor Cell Detection on the MassARRAY® System. Food and Beverage. Life Sciences. Spectroscopy. Buying Guides. Latest Editorial Buying Guides Mass Spectrometry. Liquid Handling. Microscopy. Clinical Chemistry Analyzers. Clinical Chemistry Analyzers. Clinical Laboratory Automation. DNA and RNA Purification. Flow Cytometry. Upcoming Featured Events BIOTECHNICA 2015 06 Oct 2015-08 Oct 2015 Neuroscience 2015 17 Oct 2015-21 Oct 2015 ASCB 2015 12 Dec 2015-16 Dec ...
http://selectscience.net/events-conferences/12th-hupo-world-congress/?eventID=4015
*  Techniques
... UCL Home. Staff. UCL Division of Medicine UCL School of Life and Medical Sciences. UCL Home. Nephrology. Techniques. Centre for Nephrology Home. Techniques. Staff. Contact Us. Home - Division of Medicine. Nephrology is part of the Division of Medicine. Wellcome Trust Strategic Award in Functional Neuromics Published: Jul 27, 2015 11:56:12 AM. Improving Treatment for Systemic Amyloidosis Published: Jul 20, 2015 3:05:08 PM. UCL/Rosetrees Interdisciplinary Symposium - 22nd October 2015 Published: Jul 15, 2015 4:18:43 PM. View all articles. Techniques PHYSIOLOGY Principal Investigator: Professor Robert Unwin, Dr Joanne Marks, Dr Anselm Zdebik Renal micropuncture techniques In vivo and isolated gut loop transport techniques Brush border membrane vesicle uptake technique Electrophysiology: twin electrode voltage clamp TEVC , ion-selective microelectrodes, TEVC combined with pH in oocytes, calcium and pH imaging in mammalian cells Extracellular recording of neuronal activity 2-photon calcium imaging Expression ...
http://ucl.ac.uk/medicine/nephrology/techniques
*  new proteomics publication
... carsten kettner ckettner at beilstein institut de fri jul est previous message new proteomics publication next message new proteomics publication messages sorted by dima klenchin schrieb formally the term proteomics was coined to ease getting more money for whatever protein related research fanciful name new more chances to be funded apart from that and trying to understand new realities i d suggest the following answer proteomics is the same old anything about proteins but with one twist proteomics is mindless that is it is not hypothesis driven and it does not aim to understand anything specifically instead its intent is to make a cataloog of features expression profiles d maps d structures sequences etc ect ect which will be useful for all other protein related research kinda like genome sequencing projects pretty boring and bringing very little new knowledge but unquestionably useful in many respects dima where can i sign this statement carsten previous message new ...
http://bio.net/bionet/mm/proteins/2001-July/009533.html
*  Arrayit Corporation ARYC - Arrayit Corporation Completes Proteomics Platform Installation and Traini
... ng at the National Institutes of Health - Microarrays Life Sciences Diagnostics Healthcare. Arrayit Corporation ARYC leads and empowers the genetic, research, pharmaceutical, and diagnostic communities through the discovery, development and manufacture of proprietary life science technologies and consumables for disease prevention, treatment and cure. Corporate - Media Press - Press Release. Arrayit Corporation Completes Proteomics Platform Installation and Training at the National Institutes of Health. Cytokine Protein Microarrays Advance Study of Autoimmune Diseases and Neuropyschiatric Disorders. Press Release Source: Arrayit Corporation On Monday September 20, 2010, 12:55 pm. SUNNYVALE, Calif., Sept. 20, 2010 GLOBE NEWSWIRE -- Arrayit Corporation OTCBB: ARYC - News has completed installation and technical training of its proprietary microarray platform at the National Institute of Mental Health, National Institutes of Health, in Rockville, Maryland. In 2010, the NIH will invest more than $31.2 ...
http://arrayit.com/Arrayit_Corporation_ARYC_Corpo/Microarray_Media_-_Press/Arrayit_Corporation_Completes_/arrayit_corporation_completes_proteomics_platform_installation_a.html
*  Bird’s-Eye Proteomics | The Scientist Magazine®
The Scientist Magazine. Advertisement. The Scientist. Magazine. We were expecting 10 15 proteoforms, says Heck, the head of biomolecular mass spectrometry and proteomics at Utrecht University. Top-down analyses, in which researchers feed intact proteins directly into mass spectrometers, capture the distinct characteristics of each one-of-a-kind proteoform. Molecular analysis should be done top-down, says Neil Kelleher, professor of molecular biosciences and chemistry at Northwestern University, who has been pioneering top-down proteomics for more than 20 years. THERMO FISHER SCIENTIFIC Machines featuring Orbitraps are the newest type of mass spectrometer, first commercialized in 2005 by Thermo Fisher, and the instruments are taking the top-down proteomics community by storm. In the case of the upgraded Orbitraps, this means researchers can pinpoint posttranslational modifications down to specific residues by comparing the m/z of the different fragments. Andreas Huhmer, proteomics ...
http://the-scientist.com/?articles.view/articleNo/40248/title/Bird-s-Eye-Proteomics/
*  MPI CE: Mass Spectrom. / Proteomics
... Contact. Travel. Sitemap. Impressum English. Deutsch Institute. Departments. IMPRS. Press. Datebook. Institute. Departments. Entomology. Detoxification in Insects. Olfactory Coding. Molecular Ecology. Mass Spectrom. / Proteomics. Publications. Group Members. Publications. Library. Open Positions. epso ISCE ecro Beutenberg Campus e.V. Research Group Mass Spectrometry/Proteomics. The Mass Spectrometry and Proteomics research group is in charge of the MS laboratory of the institute. The primary objective is to measure mass spectra MS for structural elucidation and new compound characterization molecular composition in cooperation with the scientific departments of the MPI-CE. In addition to service, which is aimed especially at structure confirmation of synthetic compounds, projects on structure elucidation of natural products and the elucidation of biosynthetic pathways are carried out. Strong attention is put on teaching and training our users, and we have been organizing summer ...
http://ice.mpg.de/ext/mass-spectrometry.html
*  NIH VideoCast - If you had all of the proteomics information in the world, what would you do with it
... Skip Navigation Search. CIT can broadcast your seminar, conference or meeting live to a world-wide audience over the Internet as a real-time streaming video. The event can be recorded and made available for viewers to watch at their convenience as an on-demand video or a downloadable podcast. CIT can also broadcast NIH-only or HHS-only content. VideoCast Home. FAQs. Search. Podcasts. RSS. Contact Us. Test Computer. Home. New Features. If you had all of the proteomics information in the world, what would you do with it. Loading video... Tweet. Air date: Friday, October 03, 2008, 10:00:00 AM Time displayed is Eastern Time, Washington DC Local. Views: Total views: 19 * This only includes stats from October 2011 and forward. Category: Proteomics. Runtime: 01:12:28. Description: Proteomics has generated a large amount of data about the proteins present in many types of biological samples. The informatics problems of storing and a presenting this large volume of data in a form that is ...
http://videocast.nih.gov/summary.asp?Live=7197
*  Genomics And Proteomics Reagents, Research Kits and Analytical Instruments Market
... Go. Blog. Articles. Press Releases USA - Canada Toll Free 866-552-3453. CATEGORIES. Pharmaceutical. Medical Devices. Chemical Materials. Technology Media. Energy Mining. Food Beverages. Semiconductor Electronics. Consumer Goods. . Home Biotechnology Genomics And Proteomics Reagents, Research Kits and Analytical Instruments Market Sample Preparation, Electrophoresis, BioChips and Microarrays, Chromatography, Mass Spectrometry, Thermal Cyclers, Next-Gen Sequencing - Global Industry Analysis, Size, Share, Growth, Trends and Forecast, 2013 - 2019. Press Release Genomics and Proteomics Reagents, Research Kits, and Analytical Instruments Market Driven by Drug Discovery and Disease Identification. Genomics And Proteomics Reagents, Research Kits and Analytical Instruments Market Sample Preparation, Electrophoresis, BioChips and Microarrays, Chromatography, Mass Spectrometry, Thermal Cyclers, Next-Gen Sequencing - Global Industry Analysis, Size, Share, Growth, Trends and Forecast, 2013 - ...
http://transparencymarketresearch.com/proteomics-market.html
*  Professional Quality Mascot Costumes – Tagged "cat mascots" – Starcostumes
Professional Quality Mascot Costumes Tagged cat mascots Starcostumes. . Log in or Create an account 0 item $ 0.00 · Check out Search. $ 0.00. Makeup. Foundations. Face Powders. Face Painting. Adhesives & Spirit Gum. Clown Makeup. Makeup Kits & Palettes. Hair Color. Tools & Brushes. Concealers. Special Effects Makeup. Hats. Animal Hats. Character Hats. Cowboy Hats. Crowns & Tiaras. Cultural Hats. Fun & Funny Hats. Holiday Hats. Historical Hats. Helmets. Mini Hats. Top Hats. Mascots. Bear Mascots. Bees, Hornets & Other Insects. Bird Mascots. Cat Mascots. Character Mascots. Exotic Animal Mascots. Farm Animal Mascots. Forest Animal Mascots. Holiday Mascots. Mascot Accessories. Monkey & Gorilla Mascots. Reptile & Amphibian Mascots. Sea Animal Mascots. Renaissance. Women's. Men's. Accessories. Accessories. Masks. Wigs. Props. Costumes. Men's. Women's. Boys'. Girls'. . Mascot Costumes. Browse by Tag All ape mascots bear mascots bee mascots bird mascots bobcat mascots bunny mascots cat mascots character mascots ...
http://starcostumes.com/collections/mascot-costumes/cat-mascots
*  Place of pattern in proteomic biomarker discovery. | Broad Institute of MIT and Harvard
Place of pattern in proteomic biomarker discovery. Broad Institute of MIT and Harvard. Partnerships Philanthropy Careers Contact Us. What is Broad. Our Approach Areas of Focus History Leadership Who is Broad Partner Institutions Artist-in-Residence Media Center. Press Room News from the Broad Photos for Journalists Spotlight: Ebola Spotlight: CRISPR BroadMinded Blog Video Library For the Scientific Community. Scientific Publications Science Data Software. Scientific Publications Science Data Software. Home. News & Publications:Scientific Publications Place of pattern in proteomic biomarker discovery. Recent Broad Publications A non-synonymous single-nucleotide polymorphism associated with multiple sclerosis risk affects the EVI5 interactome. Read More / View Supplemental Materials An integrated map of structural variation in 2,504 human genomes. Read More / View Supplemental Materials Somatic mutation in single human neurons tracks developmental and transcriptional history. Read More / View Supplemental ...
https://broadinstitute.org/publications/broad3687
*  Professional Quality Mascot Costumes – Starcostumes
Professional Quality Mascot Costumes Starcostumes. . Log in or Create an account 0 item $ 0.00 · Check out Search. $ 0.00. Makeup. Foundations. Face Powders. Face Painting. Adhesives & Spirit Gum. Clown Makeup. Makeup Kits & Palettes. Hair Color. Tools & Brushes. Concealers. Special Effects Makeup. Hats. Animal Hats. Character Hats. Cowboy Hats. Crowns & Tiaras. Cultural Hats. Fun & Funny Hats. Holiday Hats. Historical Hats. Helmets. Mini Hats. Top Hats. Mascots. Bear Mascots. Bees, Hornets & Other Insects. Bird Mascots. Cat Mascots. Character Mascots. Exotic Animal Mascots. Farm Animal Mascots. Forest Animal Mascots. Holiday Mascots. Mascot Accessories. Monkey & Gorilla Mascots. Reptile & Amphibian Mascots. Sea Animal Mascots. Renaissance. Women's. Men's. Accessories. Accessories. Masks. Wigs. Props. Costumes. Men's. Women's. Boys'. Girls'. . Mascot Costumes. Browse by Tag All ape mascots bear mascots bee mascots bird mascots bobcat mascots bunny mascots cat mascots character mascots chicken mascots ...
http://starcostumes.com/collections/mascot-costumes
*  Immunoproteomics
... is the study of large sets of protein s proteomics involved in the immune response examples of common applications of immunoproteomics include the isolation and mass spectrometric identification of mhc major histocompatibility complex binding peptide s purification and identification of protein antigen s binding specific antibodies or other affinity reagent s and comparative immunoproteomics to identify proteins and pathways modulated by a specific infectious organism disease or toxin an important aspect to proteomics in general is that mass spectrometry is the ultimate technique used for protein identification further reading further reading on category branches of immunology category genomics category proteins category proteomics
https://en.wikipedia.org/wiki/Immunoproteomics
*  Alexey I Nesvizhskii
2004 A statistical model for identifying proteins by tandem mass spectrometry Alexey I Nesvizhskii Institute for Systems Biology, 1441 North 34th Street, Seattle, Washington 98103, USA Anal Chem 75:4646-58. Evaluation of tandem mass spectrometry methodologies for large-scale protein analysis, and the application of s Priska D von Haller Institute for Systems Biology, 1441 North 34th Street, Seattle, WA 98103, USA Mol Cell Proteomics 2:428-42. A statistical model for identifying proteins by tandem mass spectrometry Alexey I Nesvizhskii Institute for Systems Biology, 1441 North 34th Street, Seattle, Washington 98103, USA Anal Chem 75:4646-58. Evaluation of tandem mass spectrometry methodologies for large-scale protein analysis, and the application of s Priska D von Haller Institute for Systems Biology, 1441 North 34th Street, Seattle, WA 98103, USA Mol Cell Proteomics 2:428-42. Analysis and validation of proteomic data generated by tandem mass spectrometry Alexey I Nesvizhskii University of ...
http://labome.org/expert/usa/institute/nesvizhskii/alexey-i-nesvizhskii-405342.html
*  Browsing Proteomics Center (MU) by Author "Ganapathy, Ashwin"
Browsing Proteomics Center MU by Author "Ganapathy, Ashwin". Toggle navigation. Shared more. Cited more. Safe forever. advanced search. submit works. about. help. contact us. login. Toggle navigation. Browsing Proteomics Center MU by Author. MOspace Home. University of Missouri-Columbia. Christopher S. Bond Life Sciences Center MU. Proteomics Center MU. Browsing Proteomics Center MU by Author. MOspace Home. University of Missouri-Columbia. Christopher S. Bond Life Sciences Center MU. Proteomics Center MU. Browsing Proteomics Center MU by Author. JavaScript is disabled for your browser. Some features of this site may not work without it. Search MOspace. This Community. Browse. All of MOspace Communities Collections Date Issued Author Author/Contributor Title Subject Identifier Thesis Department Thesis Advisor Thesis Semester This Community Date Issued Author Author/Contributor Title Subject Identifier Thesis Department Thesis Advisor Thesis Semester. my account. login ...
https://mospace.umsystem.edu/xmlui/handle/10355/3236/browse?value=Ganapathy, Ashwin&type=author
*  ARS | Publication request: A Comparison of Protein Extraction Methods Suitable for Gel-Based Proteom
Publication request: A Comparison of Protein Extraction Methods Suitable for Gel-Based Proteomics Studies of Aphid Proteins ARS : Research. ARS Office of International Research Programs. Title: A Comparison of Protein Extraction Methods Suitable for Gel-Based Proteomics Studies of Aphid Proteins Authors. Submitted to: Journal of Biomolecular Techniques Publication Type: Peer Reviewed Journal Publication Acceptance Date: April 2, 2009 Publication Date: September 1, 2009 Citation: Cilia, M., Fish, T., Yang, X., Mclaughlin, M., Thannhauser, T.W., Gray, S.M. A Comparison of Protein Extraction Methods Suitable for Gel-Based Proteomics Studies of Aphid Proteins. To examine protein diversity, approximately 600 proteins from aphids were identified using mass spectrometry, a technique that accurately measures the mass of individual proteins. Only one of the three methods provided both significant protein diversity and quantitative reliability, but each method also identified unique proteins. ...
http://ars.usda.gov/research/publications/publications.htm?SEQ_NO_115=231625
*  JCI - Platelet genomics and proteomics in human health and disease
... About For authors Alerts Advertise Subscribe Contact. Current Issue. Past Issues. By specialty Autoimmunity Cardiology Gastroenterology Immunology Metabolism Nephrology Neuroscience Oncology Pulmonology Vascular biology All... Videos Conversations with Giants in Medicine Author's Takes. Reviews. Reviews View all reviews... Review Series Cancer Immunotherapy Sep 2015 Autoimmunity Jun 2015 Enteric Nervous System Mar 2015 Autophagy Jan 2015 Gut Microbiome Oct 2014 Nephrology Jun 2014 Lymphatic Vasculature Mar 2014 View all review series... Collections Recently published Commentaries Editorials Hindsight The Attending Physician First Author Perspectives Scientific Show Stoppers Opinion Top read articles. Clinical Medicine JCI This Month Current issue Past issues. The Journal of Clinical Investigation. About For authors Current issue Past issues By specialty Subscribe Alerts Advertise Contact us Videos Conversations with Giants in Medicine Author's Takes Collections Commentaries Editorials Hindsight ...
http://jci.org/articles/view/26885
*  Isobaric tag for relative and absolute quantitation
... thumbnail|8plex iTRAQ kit 'Isobaric tags for relative and absolute quantitation' 'iTRAQ' is an isobaric labeling method used in quantitative proteomics by tandem mass spectrometry to determine the amount of protein s from different sources in a single experiment. It uses stable isotope labeled molecules that can be covalent bond ed to the N-terminus and side chain amine s of proteins. Procedure Data evaluation References Further reading. The ITRAQ method is based on the covalent labeling of the N-terminus and side chain amine s of peptide s from protein digestions with tags of varying mass. There are currently two mainly used reagents: 4-plex and 8-plex, which can be used to label all peptides from different samples/treatments. 4 These samples are then pooled and usually fractionated by liquid chromatography and analyzed by tandem mass spectrometry MS/MS. A database search is then performed using the fragmentation data to identify the labeled peptides and hence the corresponding proteins. The ...
https://en.wikipedia.org/wiki/Isobaric_tag_for_relative_and_absolute_quantitation
*  Standard Materials for Clinical Proteomics | GEN Magazine Articles | GEN
Market & Tech Analysis. Standard Materials for Clinical Proteomics Chemical Labeling Offers Method for Whole Proteome Absolute Quantitation Peter Schulz-Knappe. Based on recent developments in quantitative mass spectrometry, biological reference materials have been generated that can be used as internal quality controls for proteomics utilizing the characteristics of isotope-dilution mass spectrometry ID-MS. Usually synthetic or recombinant proteins are used as isotopic standards and spiked into samples to serve as internal standards for quantification. In general, two different methods of chemical labeling are used—isotopic mass labeling and isobaric mass labeling. Isotopic mass labeling leads to a distinct mass difference of structurally identical reference and target analyte. Isobaric mass labeling leads to isobaric analytes that can be differentiated by isotopic mass reporters released from the analyte during tandem mass spec. The Tandem Mass Tag® TMT® core structure invented by Proteome ...
http://genengnews.com/gen-articles/standard-materials-for-clinical-proteomics/2611/?kwrd=Biomarkers
*  Browsing Proteomics Center (MU) by Author "Chen, Mingjie"
Browsing Proteomics Center MU by Author "Chen, Mingjie". Toggle navigation. Shared more. Cited more. Safe forever. advanced search. submit works. about. help. contact us. login. Toggle navigation. Browsing Proteomics Center MU by Author. MOspace Home. University of Missouri-Columbia. Christopher S. Bond Life Sciences Center MU. Proteomics Center MU. Browsing Proteomics Center MU by Author. MOspace Home. University of Missouri-Columbia. Christopher S. Bond Life Sciences Center MU. Proteomics Center MU. Browsing Proteomics Center MU by Author. JavaScript is disabled for your browser. Some features of this site may not work without it. Search MOspace. This Community. Browse. All of MOspace Communities Collections Date Issued Author Author/Contributor Title Subject Identifier Thesis Department Thesis Advisor Thesis Semester This Community Date Issued Author Author/Contributor Title Subject Identifier Thesis Department Thesis Advisor Thesis Semester. my account. login ...
https://mospace.umsystem.edu/xmlui/handle/10355/3236/browse?value=Chen, Mingjie&type=author
*  Browsing Proteomics Center (MU) by Subject
Browsing Proteomics Center MU by Subject. Toggle navigation. Shared more. Cited more. Safe forever. advanced search. submit works. about. help. contact us. login. Toggle navigation. Browsing Proteomics Center MU by Subject. MOspace Home. University of Missouri-Columbia. Christopher S. Bond Life Sciences Center MU. Proteomics Center MU. Browsing Proteomics Center MU by Subject. MOspace Home. University of Missouri-Columbia. Christopher S. Bond Life Sciences Center MU. Proteomics Center MU. Browsing Proteomics Center MU by Subject. JavaScript is disabled for your browser. Some features of this site may not work without it. Search MOspace. This Community. Browse. All of MOspace Communities Collections Date Issued Author Author/Contributor Title Subject Identifier Thesis Department Thesis Advisor Thesis Semester This Community Date Issued Author Author/Contributor Title Subject Identifier Thesis Department Thesis Advisor Thesis Semester. my account. login register non-UM ...
https://mospace.umsystem.edu/xmlui/handle/10355/3236/browse?type=subject
*  Peptide spectral library
... The process of peptide/protein identification is called spectral library searching. Shotgun proteomics Protein identification via sequence database searching Shortcomings of the sequence database searching workflow. Research community focused peptide spectral Libraries Cardiac-Specific Organellar Protein Atlas Knowledgebase. In a shotgun proteomics approach, proteins in a complex mixture are digested by proteolytic enzymes such as trypsin. To acquire tandem mass spectra, a particular peptide precursor is isolated, and fragmented in a mass spectrometer ; the mass spectra corresponding to the fragments of peptide precursor is recorded. Tandem mass spectra contains specific information regarding the sequence of the peptide precursor, which can aid the identification of peptide/protein. Protein identification via sequence database searching. Sequence database searching is widely used currently for mass spectra based protein identification. 1994 An approach to correlate tandem mass-spectral ...
https://en.wikipedia.org/wiki/Peptide_spectral_library
*  Immunoproteomic analysis of potential serum biomarker candidates in human glaucoma.
... MedLine Citation:. For high-throughput characterization of antigens, serum IgG was eluted from five randomly selected glaucomatous samples and analyzed by linear ion trap mass spectrometry LC-MS/MS. Serum titers of selected biomarker candidates were then measured by specific ELISAs in the whole sample pool including an additional control group of diabetic retinopathy. RESULTS: LC-MS/MS analysis of IgG elutes revealed a complex panel of proteins, including those detectable only in glaucomatous samples. Interestingly, many of these antigens corresponded to upregulated retinal proteins previously identified in glaucomatous donors or that exhibited increased methionine oxidation. As a narrowing-down strategy for selection of initial biomarker candidates, we determined the serum proteins overlapping with the retinal proteins known to be up-regulated in glaucoma. CONCLUSIONS: A number of serum proteins identified by this immunoproteomic study of human glaucoma may represent diseased tissue-related antigens ...
http://biomedsearch.com/nih/Immunoproteomic-Analysis-Potential-Serum-Biomarker/23150628.html
*  Proteomics Market (Protein Microarray, Mass Spectrometry, NMR Spectroscopy) Global Forecast to 2018
Proteomics Market Protein Microarray, Mass Spectrometry, NMR Spectroscopy Global Forecast to 2018. Proteomics Market Instruments, Reagents Services Forecasts to 2018. Dallas, TX -- SBWIRE -- 04/01/2014 -- RnRMarketResearch.com adds “Proteomics Market Protein Microarray, Mass Spectrometry, NMR Spectroscopy, Chromatography, Electrophoresis, Surface Plasmon Resonance, X-ray Crystallography Instruments, Reagents & Services – Global Forecast to 2018" report to its research database. Complete Report Available @ http://www.rnrmarketresearch.com/proteomics-market-protein-microarray-mass-spectrometry-nmr-spectroscopy-chromatography-electrophoresis-surface-plasmon-resonance-protein-fractionation-x-ray-crystallography-immunoassay-ins-market-report.html. Personalized medicine is one of the niche markets, and has witnessed a significant growth rate in the past few years. Thus, further growth in the personalized medicine market is reckoned as one of the strongest growth drivers for the global ...
http://sbwire.com/press-releases/proteomics-market-instruments-reagents-services-forecasts-to-2018-485935.htm
*  Proteomics/Protein Separations - Centrifugation/Preparative Centrifugation vs. Analytical Centrifuga
... tion - Wikibooks, open books for an open world. Proteomics/Protein Separations - Centrifugation/Preparative Centrifugation vs. Analytical Centrifugation From Wikibooks, open books for an open world Proteomics. Protein Separations - Centrifugation This page may need to be reviewed for quality. Jump to: navigation, search Table of Contents: Protein Separations - Centrifugation. History of the Centrifuge How the Centrifuge Works Density Gradient Centrifugation Differential Centrifugation Preparative Centrifugation vs. Analytical Centrifugation. Contents. 1 Two types of ultracentrifuges developed: analytical and preparative 1.1 Analytical 1.2 Preparative. Two types of ultracentrifuges developed: analytical and preparative. Analytical. · Uses small sample size less than 1 ml. · Built in optical system to analyze progress of molecules during centrifugation. · Uses relatively pure sample. · Used to precisely determine sedimentation coefficient and MW of molecules. · Beckman Model E is an example ...
https://en.wikibooks.org/wiki/Proteomics/Protein_Separations_-_Centrifugation/Preparative_Centrifugation_vs._Analytical_Centrifugation
*  Functional proteomic screen identifies a modulating role for CD44 in death receptor-mediated apoptos
... is. Document Detail. Functional proteomic screen identifies a modulating role for CD44 in death receptor-mediated apoptosis. MedLine Citation:. FALI of the Fas receptor Fas/CD95 using a fluorescein-conjugated anti-Fas antibody abrogated Fas ligand-mediated caspase activation. One of the targets of these positive scFvs was identified as CD44 and was validated by performing FALI using a CD44-specific monoclonal antibody, which resulted in similar protection from Fas apoptosis. CD44-targeted FALI was antiapoptotic in multiple human cancer cell lines, including both Fas signaling type I and II cells, and was also protective against other ligands of the tumor necrosis factor death receptor family. FALI of CD44 inhibited formation and activation of the death-inducing signaling complex, suggesting that CD44 regulates Fas at the cell surface. 11870367 - Cox-2 inhibits fas-mediated apoptosis in cholangiocarcinoma cells. 23452257 - Differential effects of cxcr4-cxcl12- and cxcr7-cxcl12-mediated immune reactions on ...
http://biomedsearch.com/nih/Functional-proteomic-screen-identifies-modulating/15753387.html
*  Molecular & Cellular Proteomics
molecular cellular proteomics molecular cellular proteomics molecular cellular proteomics is a monthly peer reviewed scientific journal established in and published by the american society for biochemistry and molecular biology it covers research on structural and functional properties of protein s especially with regard to development scope history abstracting and indexing references external links scope history the journal also publishes other content such as hupo views which are reports from the human proteome organization hupo proceedings from hupo meetings and the proceedings of the international symposium on mass spectrometry in the life sciences as of january the journal is published online only and no longer available in print the editors in chief are a l burlingame and ralph a bradshaw all articles are available free year after publication in press articles are available free on its website immediately after acceptance abstracting and indexing mcp is indexed in medline pubmed ...
https://en.wikipedia.org/wiki/Molecular_&_Cellular_Proteomics
*  .. Services .. contact Us
Skip to Content Meharry Medical College. About Meharry Education Research Patient Care Students. Meharrians Find a Doctor Campus Calendar Contact Us. Search the Site Search. Centers Sponsored Research Research by School Office for Research Research Publications For Researchers Clinical Trials. Services LC-MS/MS Analysis: Liquid Chromotography Multistage Mass Spectrometry. Basic Analysis includes sample preparation and identification of trypsinized protein samples. Shotgun Proteomics: profiling and identification of complex protein samples. Protein Identification - LC/MS/MS analyses from gel bands or spots. Small molecule LC/MS Analysis : LC/MS and LC/MS/MS analyses qualitative and quantitative of small bio-molecules peptides, drugs, metabolites, vitamins, etc. Additional Proteomics Services : 2D Differential In-Gel Electrophoresis DIGE, Targeted Mass Spectrometry and Multidimensional LC/MS/MS MudPIT – available via the partnerships with the MSRC Proteomics Lab at the Vanderbilt ...
http://mmc.edu/research/office-for-research/clinical-research/core-facilities/proteomics/services.html
*  Proteomics & Protein Research | GenomeWeb
Proteomics & Protein Research. GenomeWeb. Skip to main content. RSS Feeds Twitter LinkedIn. Log in Join now. Business Policy. Business News Research Funding Policy Legislation Regulatory News Reimbursement. Technology. Microarrays Multiplexing PCR Informatics Sequencing Technology Mass Spec Sample Prep Gene Silencing/Gene Editing. Research. Genetic Research Gene Expression Research Epigenetics Research Proteomics Protein Research Cell Biology Research. Clinical. Molecular Diagnostics Companion Diagnostics Biomarker Discovery Validation Drug Discovery Development Clinical Sequencing Clinical Proteomics. Disease Areas. Cancer Infectious Disease Cardiovascular Disease Neurological Psychological Disease Metabolic Disease Autoimmune Disease Inherited Disease Reproductive Health. Applied Markets. Resources. Webinars White Papers The Scan Career Blog Job Listings IP Update New Products People in the News Conferences Events. Main menu. Enter your keywords. Home » Research » Proteomics ...
https://genomeweb.com/research/proteomics-protein-research?page=8
*  Proteomic analysis of the mitochondria-enriched fraction from diabetic rat skeletal muscle - Mayno
Proteomic analysis of the mitochondria-enriched fraction from diabetic rat skeletal muscle - Maynooth University ePrints and eTheses Archive. Maynooth University ePrints and eTheses Archive. Browse. Search. Register. User Area. Help. Statistics. eTheses. Login. Proteomic analysis of the mitochondria-enriched fraction from diabetic rat skeletal muscle. Mullen, Edel and Ohlendieck, Kay 2011 Proteomic analysis of the mitochondria-enriched fraction from diabetic rat skeletal muscle. Journal of Integrated Omics, 1 1. ISSN 2182-0287. Abstract Mitochondrial dysfunction in muscle has been implicated to play a causative role or being an indirect consequence of insulin resistance in type-2 diabetes. In order to investigate potential diabetes-related alterations in the mitochondrial proteome of muscle, we have carried out a mass spectrometry-based proteomic analysis of the gastrocnemius muscle from normal versus diabetic Goto-Kakizaki rats. A generally perturbed protein expression pattern was ...
http://eprints.maynoothuniversity.ie/2465/
*  Proteomics/Proteolytic processing/Proteolytic processing- role in therapeutics - Wikibooks, open boo
... ks for an open world. Proteomics/Proteolytic processing/Proteolytic processing- role in therapeutics From Wikibooks, open books for an open world Proteomics. Proteolytic processing This page may need to be reviewed for quality. Jump to: navigation, search Proteolytic processing- role in therapeutics. The fact that certain proteins can modify others by binding to specific site and alter its activity is used as a principle in the development of certain modern therapeutics. Here is an example of how the process of proteolytic processing has been used to find possible cure for diseases. Human coronavirus is the causative agent of the common respiratory disease in adults and children. There are no current cure for CoV as a result proteolytic processing is being exploited to find new drug targets. Biochemical analysis of HCoV –NL63 virus showed 2 viral papain like protease PLP1 and PLP2 which process viral replicase polyprotein. In this case they generated polyclonal antisera against 2 of ...
https://en.wikibooks.org/wiki/Proteomics/Proteolytic_processing/Proteolytic_processing-_role_in_therapeutics
*  SILAC | Broad Institute of MIT and Harvard
Press Room News from the Broad Photos for Journalists Spotlight: Ebola Spotlight: CRISPR BroadMinded Blog Video Library For the Scientific Community. Scientific Publications Science Data Software. Scientific Publications Science Data Software. For the Scientific Community:Science. Proteomics SILAC. S table I sotope L abeling by A mino acids in C ell culture SILAC is a simple, robust, yet powerful approach in mass spectrometry MS -based quantitative proteomics. SILAC labels cellular proteomes through normal metabolic processes, incorporating non-radioactive, stable isotope containing amino acids in newly synthesized proteins. Growth medium is prepared where natural light amino acids are replaced by heavy SILAC amino acids. Cells grown in this medium incorporate the heavy amino acids after five cell doublings and SILAC amino acids have no effect on cell morphology or growth rates. Co-precipitating interacting proteins with an epitope tagged protein as the affinity bait is a popular approach to ...
http://broadinstitute.org/scientific-community/science/platforms/proteomics/silac
*  A systems approach implicates nuclear receptor targeting in the Atp7b−/− mouse model of
... Wilson's disease - Metallomics RSC Publishing. Chemistry World. Log in Register. Metallomics. Please enable JavaScript to access the full features of the site or access our non-JavaScript page. Scheduled maintenance upgrade on Monday, 5th of October from 8pm to 10pm BST Please note that we will be performing a planned maintenance upgrade on Monday 5th of October from 8pm to 10pm British Summer Time. Paper A systems approach implicates nuclear receptor targeting in the Atp7b −/− mouse model of Wilson's disease. a Dept. This content is free, please choose one of the three options provided in the Log in section to gain access. Please choose one of the options provided in the log in section to gain access to this content:. Wilson's disease WD is an inherited disorder of copper metabolism characterized by liver disease and/or neurologic and psychiatric pathology. Studies in the Atp7b −/− mouse model of WD revealed specific transcript and metabolic changes that precede development of liver pathology, most ...
http://pubs.rsc.org/en/Content/ArticleLanding/2012/MT/C2MT20017A
*  Rolf Apweiler
... {{Infobox scientist name = Rolf Apweiler image = image size =. alt =. caption = Do you have a picture of Rolf Apweiler. Please, post here. birth date =. birth place =. death date = death place =. residence =. citizenship =. nationality =. fields = 'Rolf Apweiler' is a director of European Bioinformatics Institute EBI part of the European Molecular Biology Laboratory EMBL https://www.ebi.ac.uk/about/people/rolf-apweiler Rolf Apweiler page at the EBI http://uk.linkedin.com/pub/rolf-apweiler/b/44/380 Rolf Apweiler LinkedIn profile with Ewan Birney. 14. Education Research Awards and honours References. Education. Apweiler gained his PhD in Biochemistry from Heidelberg University. 15. http://pir.uniprot.org/help/apweiler Rolf Apweiler page at UniProt.org. Research. Apweiler has been working on the Swiss-Prot. 16. protein sequence database since 1987, and in 1994 he became leader of the Swiss-Prot group. He has been joint head of the Protein and Nucleotide Data PANDA Group since 2007. The PANDA group is ...
https://en.wikipedia.org/wiki/Rolf_Apweiler
*  Mass Spectrometry-Based Proteomics in Molecular Diagnostics: Discovery of Cancer Biomarkers Using Ti
mass spectrometry based proteomics in molecular diagnostics discovery of cancer biomarkers using tissue culture figure figure a schematic overview of silac cells are grown in normal and heavy amino acids containing media for generations to achieve maximal incorporation of heavy amino acids the proteins are extracted from both populations of cells and mixed in equal proportion and then subjected to either in gel or in solution digestion relative abundance of the digested peptides is determined from the ratio of heavy to light peptide signals as obtained from ms
http://hindawi.com/journals/bmri/2013/783131/fig5/
*  Support & feedback < EMBL-EBI
Support feedback EMBL-EBI. Skip to main content. Skip to local navigation. Skip to EBI global navigation menu. Skip to expanded EBI global navigation menu includes all sub-sections. Services. Research. Training. Industry. About us. . Services Support feedback. Overview. A to Z. Service teams. Data submission. Support. Services Support. If you are having problems using one of the EMBL-EBI services or tools, or if you would just like to send us some feedback, please use the contact form below. Support Feedback Your E-mail address. Topic. General Feedback or Please Select Sign up for usability testing. CRAM Database of Genomic Variants archive DGVa EMBL-Bank ENA Sequence Version Archive SVA. European Genome-phenome Archive EGA European Nucleotide Archive ENA European Variation Archive EVA IMGT/HLA Immuno Polymorphism Database IPD Metagenomics. Webin. ArrayExpress European Genome-phenome Archive EGA Expression Atlas MetaboLights: Metabolomics archive and reference database PRoteomics IDEntifications ...
http://ebi.ac.uk/support/sequences
*  Edward Marcotte
... 'Edward Marcotte' is a professor of biochemistry at The University of Texas at Austin, working in genetics, proteomics, and bioinformatics. Zimmer, Carl., 'The New York Times', New York, April 26, 2010. Marcotte is an example of a computational biologist who also relies on experiment s to validate bioinformatics-based predictions. Marcotte's undergraduate education was at The University of Texas at Austin, where he received a B.S. in Biochemistry from The University of Texas at Austin in 1995, and did his postdoctoral work both at UT Austin and at University of California, Los Angeles with Professor David Eisenberg. Marcotte has been a professor at UT Austin since 2001. Marcotte's major research contributions are in the areas of bioinformatics, proteomics, systems biology, and synthetic biology. Bioinformatics and systems biology. In early work, Marcotte and colleagues created the first genome-scale map of functional links among proteins in any complex organism the yeast Saccharomyces ...
https://en.wikipedia.org/wiki/Edward_Marcotte
*  DROPS - Document
... License When quoting this document, please refer to the following URN: urn:nbn:de:0030-drops-5341 URL: http://drops.dagstuhl.de/opus/volltexte/2006/534/. An Algorithm for Feature Finding in LC/MS Raw Data. Abstract Liquid chromatography coupled with mass spectrometry is an established method in shotgun proteomics. A key step in the data processing pipeline is to transform the raw data acquired by the mass spectrometer into a list of features. In this context, a emph{feature} is defined as the two-dimensional integration with respect to retention time RT and mass-over-charge m/z of the eluting signal belonging to a single charge variant of a measurand e.g., a peptide. Features are characterized by attributes like average mass-to-charge ratio, centroid retention time, intensity, and quality. We anticipate that our automatic quantitation method will facilitate further absolute or relative quantitation of even more complex peptide samples. The algorithm was implemented in the publicly available ...
http://drops.dagstuhl.de/opus/frontdoor.php?source_opus=534
*  Education
... - Quick Links - UAB Home - Academic Calendar - AdminSystems - Apply - AskIT - Blackboard - BlazerNET: Students Faculty - Campus Calendar - Campus Map - Departments - Directory - Email - Emergency/B-Alert - Employee Resources - Employment - Give to UAB - Research Administration. Services. Education Protocols. Research Team. Contact Us. Services. Education Protocols. Education. Protocols. Research Team. Contact Us. Calendar. Education Protocols Education Education A few examples, i.e. presentations and links to movies and sites to help get you up to par with the more common instruments and approaches. Mass Spectrometry MS Common to Proteomics Research: Different Mass Spectrometry Types i.e. MALDI vs. SELDI, Wiki Definitions MALDI-TOF-MS Profiling Example MALDI Biotyper by Bruker Daltronics MALDI Vs. ESI John Fenn Lecture MALDI-TOF/TOF-MS ESI-LT-MS Example of Linear Ion Trap by Thermo Finnigan FTICR-MS Ion Mobility Mass Spectrometry Overview of Synapt HDMS by Waters. A Few MS Procedures ...
http://uab.edu/proteomics/bmsf/educationprotocols/education.php
*  Gpm (proteomics)
gpm proteomics gpm proteomics multiple issues in proteomics gpm stands for global proteome machine it is a web based open source user interface for analyzing and displaying protein identification data it was originally designed by rob craig and ron beavis the interface creates a series of web browser page views of tandem mass spectrometry data that has been assigned to protein sequences the underlying data documents are stored in bioml format files references external links category proteomics
https://en.wikipedia.org/wiki/Gpm_(proteomics)
*  Applied Proteomics raises $28 million | SanDiegoUnionTribune.com
Applied Proteomics raises $28 million. SanDiegoUnionTribune.com. . Newsletters. Text Alerts. News +. Nation. World. Communities. . Chargers. NFL. High School / Preps. Chargers stadium. Business +. Biotech. Real Estate. Small Business. Finance. Events. Music. Classical Music. Theater Performing Arts. Visual Arts. DiscoverSD. SD Best Poll. Events. Guides. Military +. Special Forces. Letters to the Editor. Steve Breen. Steve Breen Caption Contest. News. Sports. Business. Entertainment. Features. Watchdog. Military. More. News Obituaries. Private Party Obituaries. Point Loma Diagnostics developer Applied Proteomics said Monday it has raised $28 million in venture capital funding. The La Jolla company said part of the money will be used to commercialize its blood test for warning signs of colorectal cancer. The blood test detects protein patterns that characteristically appear when colorectal cancer is forming, said Chief Executive Peter Klemm. Peter Klemm, CEO of Applied Proteomics Applied ...
http://sandiegouniontribune.com/news/2013/aug/19/applied-proteomics-raises-28-million/
*  GProX
... Welcome to the GP ro X homepage.       Home. Download. Gallery. Version history. Contact/Support  . Home       The Graphical Proteomics Data Explorer GProX is a freely available complete software platform for comprehensive and integrated analysis and visualization of large proteomics datasets. The program provides a range of functions for data analysis. Visit the gallery for application screenshots and example graphics produced by the program or go directly to the download section to get the program. To stay updated with new releases sign up for the GProX google group on the contact page. If you use results generated with GProX please acknowledge our work by citing: GProX, a User-Friendly Platform for Bioinformatics Analysis and Visualization of Quantitative Proteomics Data Rigbolt KT, Vanselow JT, Blagoev B Mol Cell Proteomics. Here you will also find detailed description of the the program features and properties.       Features ...
http://gprox.sourceforge.net/
*  High MW IgG signals in IP/co-IP with protein G - Protein and Proteomics - BioForum
... Google Sign in options. Remember me This is not recommended for shared computers Sign in anonymously Don't add me to the active users list Privacy Policy. Forums. Members. BioWiki. Quotes. BioVideo. Forums. BioWiki. Quotes. Contact Us. BioForum. Protocols and Techniques Forums. Protein and Proteomics. Javascript Disabled Detected You currently have javascript disabled. High MW IgG signals in IP/co-IP with protein G Started by. miST32, Feb 19 2014 01:04 PM IgG. Rabbit IgG. Co-IP. IP. non-specific bands. 1 reply to this topic #1. miST32. miST32. Enthusiast Active Members. 42 posts. Namely, I'm detecting high molecular weight bands in my IgG IP control. I add 5ug of normal rabbit IgG same as IP antibody, and perform the immunoprecipitation with protein G dynabeads. Has anybody else had a tough time with normal rabbit IgG in regards to these high molecular weight species on western blot. I've detected similar bands though not as robust with BS3 cross-linking of the antibody or IgG to the dynabeads, ...
http://protocol-online.org/forums/topic/31712-high-mw-igg-signals-in-ipco-ip-with-protein-g/
*  NIDA Center for Functional Genomics: Proteomics
nida center for functional genomics proteomics about publications genomics proteomics immunology modeling contact announcements new targets for the control of hiv predicted using a novel computational analysis read more hiv alters t cell rna processing and noncoding rna expression read more proteomics and epigenetics benjamin a garcia ph d princeton university the proteomics and epigenetics core provides cutting edge mass spectrometry capabilities with special expertise in the high throughput and quantitative analysis of signaling networks through measurement of protein phosphorylation protein abundance and epigenetic post translational modifications ptms the garcia lab has recently developed methods for rapid quantification of nearly all single core histone ptms and variants in a single experiment additionally the lab has created one of the only high throughput platforms for the ...
http://nida.viromics.washington.edu/proteomics.html
*  Sandra Orchard
... add/edit. You are here: Scientific Experts UK. Wellcome Trust Genome Campus Orchard Sandra Orchard. Research Topics proteomics protein databases publishing database management systems protein interaction mapping computational biology proteome information storage and retrieval genetic databases controlled vocabulary education software publications scientific societies biotechnology research proteins internationality netherlands information dissemination information management access to information post translational protein processing congresses toxicology chemical industry factual databases writing pharmacokinetics biological markers. Sandra Orchard Summary Affiliation: Wellcome Trust Genome Campus Country: UK. Publications Debunking minimum information myths: one hat need not fit all Sandra Orchard EMBL European Bioinformatics Institute, Wellcome Trust Genome Campus, Cambridge, UK N Biotechnol 25:171-2. 2009 Data standardization and sharing-the work of the HUPO-PSI Sandra Orchard EMBL ...
http://labome.org/expert/uk/wellcome/orchard/sandra-orchard-210477.html
*  KINAXO Biotechnologies and Bayer Vital GmbH Collaborate in Phosphoproteomics Biomarker Identificatio
... n. KINAXO Biotechnologies and Bayer Vital GmbH Collaborate in Phosphoproteomics Biomarker Identification. Print. Thursday, 15 October 2009 09:00 UTC + 2. . Martinsried, Leverkusen, Germany, October 15, 2009 /. b3c newswire. / - KINAXO Biotechnologies GmbH and Bayer Vital GmbH announced today that they will enter into collaboration. KINAXO will apply its quantitative phosphoproteomics technology PhosphoScout® for the identification of novel biomarkers in a clinical trial conducted by Bayer Vital. KINAXO’s phosphoproteomics platform allows annotation and quantification of regulated phosphorylation sites. Since the majority of targeted compounds used as anti-cancer drugs influence cellular signal transduction pathways, analysis of phosphorylation patterns in relation to drug administration reveals a compound’s molecular mode of action. Characteristic phosphorylation sites predicting response to treatment, resistance mechanism or synergistic effects can hereby be identified as biomarkers ...
http://b3cnewswire.com/20091015186/kinaxo-biotechnologies-and-bayer-vital-gmbh-collaborate-in-phosphoproteomics-biomarker-identification/Print.html
*  Proteomic screen defines the Polo-box domain interactome and identifies Rock2 as a Plk1 substrate. |
Proteomic screen defines the Polo-box domain interactome and identifies Rock2 as a Plk1 substrate. Partnerships Philanthropy Careers Contact Us. Our Approach Areas of Focus History Leadership Who is Broad Partner Institutions Artist-in-Residence Media Center. Press Room News from the Broad Photos for Journalists Spotlight: Ebola Spotlight: CRISPR BroadMinded Blog Video Library For the Scientific Community. Scientific Publications Science Data Software. Scientific Publications Science Data Software. News & Publications:Scientific Publications Proteomic screen defines the Polo-box domain interactome and identifies Rock2 as a Plk1 substrate. Read More / View Supplemental Materials Linking RNA biology to lncRNAs. Read More / View Supplemental Materials Ethanol Enhances TGF-β Activity by Recruiting TGF-β Receptors from Intracellular Vesicles/Lipid Rafts/Caveolae to Non-lipid Raft Microdomains. Read More / View Supplemental Materials. Scientific Publications Back to Publications Proteomic screen defines the ...
https://broadinstitute.org/publications/broad3883
*  3rd Principal Investigators Meeting Presentations and Posters- Innovation.Cancer.Gov
... National Cancer Institute. www.cancer.gov. at the National Institutes of Health. Contact Us. Home Search. Go. Download IMAT Fact Sheet. About IMAT. History. Mission. Center for Strategic Scientific Initiatives. Management Team. Scope of Supported Technologies. Outputs and Achievements. Individual Technologies and Platforms. MELT Total Nucleic Acid Isolation System. Isotope Coded Affinity Tags ICAT. Rolling Circle Amplification. Sentrix BeadChip and BeadArray Technology. Quantum Dot Streptavidin Conjugates. MudPIT Multidimensional Protein Identification Technology. Recombomice. RNA Later Derivatives. Microfluidic Genetic Analysis MGA. COLD-PCR Technology. RainDance/RDT-1000. GeneChip CustomSeq Resequencing Arrays. Program Presentations. News Archives. Annual PI Meetings. Grants Awarded. Program Areas. Technology Development. Education and Training. Communications, Outreach and Strategic Partnerships. Funding Opportunities. Funding Overview. Mechanisms of Support. Technology Development Theme Areas. Areas ...
http://innovation.cancer.gov/news/p.asp
*  3rd Principal Investigators Meeting Presentations and Posters- Innovation.Cancer.Gov
... National Cancer Institute. www.cancer.gov. at the National Institutes of Health. Contact Us. Home Search. Go. Download IMAT Fact Sheet. About IMAT. History. Mission. Center for Strategic Scientific Initiatives. Management Team. Scope of Supported Technologies. Outputs and Achievements. Individual Technologies and Platforms. MELT Total Nucleic Acid Isolation System. Isotope Coded Affinity Tags ICAT. Rolling Circle Amplification. Sentrix BeadChip and BeadArray Technology. Quantum Dot Streptavidin Conjugates. MudPIT Multidimensional Protein Identification Technology. Recombomice. RNA Later Derivatives. Microfluidic Genetic Analysis MGA. COLD-PCR Technology. RainDance/RDT-1000. GeneChip CustomSeq Resequencing Arrays. Program Presentations. News Archives. Annual PI Meetings. Grants Awarded. Program Areas. Technology Development. Education and Training. Communications, Outreach and Strategic Partnerships. Funding Opportunities. Funding Overview. Mechanisms of Support. Technology Development Theme Areas. Areas ...
http://innovation.cancer.gov/news/p_miyamoto.asp
*  Protein extremes gain relevance in massive proteomic studies -- ScienceDaily
... Your source for the latest research news. Protein extremes gain relevance in massive proteomic studies. Date: June 15, 2010 Source: Universitat Autonoma de Barcelona Summary: Researchers have developed a new procedure to identify the two extremes of protein molecules and their processing and maturing in in-vivo and ex-vivo massive proteomic studies. Researchers at University of Ghent, Belgium, and Universitat Aut noma de Barcelona UAB have developed a new procedure to identify the two extremes of protein molecules and their processing and maturing in in-vivo and ex-vivo massive proteomic studies. The new method developed by scientists helps to expand studies to C-terminal proteins -Ct, proteins with a free carboxyl group, COOH, at the end of the linear chain of amino acids. "Protein extremes gain relevance in massive proteomic studies." ScienceDaily. Protein extremes gain relevance in massive proteomic studies. "Protein extremes gain relevance in massive proteomic studies." ScienceDaily. RELATED TOPICS. ...
http://sciencedaily.com/releases/2010/06/100615105247.htm
*  Proteomics (journal)
proteomics journal proteomics journal proteomics is a peer reviewed scientific journal covering topics including whole proteome analysis of organisms protein expression profiling disease pharmaceutical agricultural and biotechnological applications and analysis of cellular systems organelles and protein complexes the impact factor was category publications established in category english language journals category bimonthly journals category proteomics journals category john wiley sons academic journals
https://en.wikipedia.org/wiki/Proteomics_(journal)
*  .. In-Cell Western™ Assay Webinar – Applications Review .. In-Cell Western™ Assay
Cancer Research, Odyssey Imaging Systems, Proteomics Applications. cell based assays, cell proliferation assays, immunofluorescent assays, in cell westerns, in-cell ELISA, licor odyssey imagers, on-cell western assays, protein phosphorylation, quantifying proteins, RNAi knockdowns, RNAi screening, signal transduction. In-Cell Western™ Assay Application: Response of COS-7 Cells to Hydroxyurea June 15, 2012. Cancer Research, IRDye Reagents, Odyssey Imaging Systems, Proteomics Applications. cell based assays, immunofluorescent assays, in-cell ELISA, licor odyssey imagers, near-infrared fluorescent reagents, odyssey applications, protein phosphorylation, signal transduction. Application: Detecting phospho-p53 in COS cells in response to Hydroxyurea. Background wells were incubated with secondary antibody but no primary antibody. IRDye ® 680RD secondary antibodies were used for detection in the 700nm channel and IRDye 800CW secondary antibodies were usd for detection in the 800nm channel. In-Cell ...
http://licor.com/bio/blog/2012/06
*  Ali R Vaezzadeh, Harvard University - ResearchGate
... Ali R Vaezzadeh. Are you Ali R Vaezzadeh. DOI:10.1016/j.bbapap.2013.05.008 4.66 Impact Factor. Vaezzadeh. Lee The Journal of Urology 04/2012; 187 4 :e300-e301. DOI:10.1093/jac/dkr195 5.31 Impact Factor. Shotgun Proteomics: A Qualitative Approach Applying Isoelectric Focusing on Immobilized pH Gradient and LC-MS/MS. Jacques M P Deshusses. Denis F Hochstrasser. Finally, they are detected by tandem mass spectrometry MS/MS and proteins are matched by means of bioinformatics software. DOI:10.1007/978-1-60761-913-0 26 1.29 Impact Factor. Denis F Hochstrasser. Finally, following detection by mass spectrometry MS and sequencing by tandem mass spectrometry MS/MS, proteins are matched by means of bioinformatics software, and protein ratios are calculated by comparing isobaric tagged reporter fragments to highlight the different expression of one protein in one sample relative to other samples. Ali R Vaezzadeh. Produced peptides were analyzed by Shotgun IPG-IEF, a methodology relying on separation of ...
http://researchgate.net/researcher/84467735_A_Vaezzadeh
*  Wiley Online Library: Search Results Page
... Article first published online : 10 NOV 2014, DOI: 10.1002/prca.201400030. Abstract. Full Article HTML. Enhanced Article HTML. PDF 973K. PDF 973K. References. Request Permissions. Proteomics in granzyme research PROTEOMICS - Clinical Applications. Volume 8, Issue 5-6, June 2014, Pages: 351 381, Lars T. Article first published online : 10 JUN 2014, DOI: 10.1002/prca.201300096. Abstract. Full Article HTML. Enhanced Article HTML. PDF 502K. PDF 502K. References. Request Permissions. Article first published online : 16 SEP 2015, DOI: 10.1002/prca.201500029. Abstract. Full Article HTML. Enhanced Article HTML. PDF 330K. PDF 330K. References. Request Permissions. Article first published online : 28 AUG 2014, DOI: 10.1002/prca.201400015. Abstract. Full Article HTML. Enhanced Article HTML. PDF 471K. PDF 471K. References. Request Permissions. You have free access to this content. Coupling enrichment methods with proteomics for understanding and treating disease PROTEOMICS - Clinical ...
http://onlinelibrary.wiley.com/advanced/search/results?articleDoi=10.1002/prca.201200042&scope=allContent&start=1&resultsPerPage=20
*  COL4A3 - Collagen alpha-3(IV) chain - human protein (Proteomics)
COL4A3 - Collagen alpha-3 IV chain - human protein Proteomics. COL4A3 - Collagen alpha-3 IV chain - human protein Proteomics. Login or Sign up now. x loading ... close X. Oooops, an error occured. COL4A3 - Collagen alpha-3 IV chain - human protein Proteomics. Home Recent activites. My favorites. My labels. Downloads Login or Sign up. x. Login Or Sign up Now. Try our new search. Protein. Function. Medical. Expression. Interactions. Localisation. Sequence. Proteomics. Structures. Identifiers. Gene. Exons. Identifiers. References. Curated publications 68. Additional publications 1. Patents 0. Submissions 2. Web resources 0. COL4A3 Collagen alpha-3 IV chain. favorite. label. cancel. Login or Sign up now. x Protein also known as:. Goodpasture antigen. Cleaved into:. Tumstatin. Gene name: COL4A3 Family name:. Type IV collagen. Entry whose protein s existence is based on evidence at protein level. Protein Recommended name Collagen alpha-3 IV chain. Alternative name Goodpasture antigen. ...
http://nextprot.org/db/entry/NX_Q01955/proteomics
*  His tag protein purification - Protein and Proteomics - BioForum
... Google Sign in options. Remember me This is not recommended for shared computers Sign in anonymously Don't add me to the active users list Privacy Policy. Sign In. Create Account. This topic. Forums. Members. BioBlog. BioWiki. Interest Groups. Quotes. BioVideo. Forums. BioBlog. BioWiki. Interest Groups. Quotes. BioForum. Protein and Proteomics. Javascript Disabled Detected You currently have javascript disabled. His tag protein purification Started by. freakyartemis, Jul 13 2009 06:46 AM. 3 replies to this topic #1. freakyartemis. freakyartemis. member Members. 2 posts. Posted 13 July 2009 - 06:46 AM I wanna purify a protein that is in vitro translated with the TNT Kit from Promega. I thought about Ni-NTA purification or magnetic Ni-NTA purification, but the protocol says that reagents such as amino acids are not recommended. My protein of interest is in vitro translated with the help of rabbit reticulocyte lysate where I add amino acids. Is there any way to purify the protein where the amino ...
http://protocol-online.org/forums/topic/9099-his-tag-protein-purification/

Proteomics Standards Initiative: The Proteomics Standards Initiative (PSI) is a working group of Human Proteome Organization. It aims to define data standards for proteomics in order to facilitate data comparison, exchange and verification.Plant Proteome Database: The Plant Proteome Database is a National Science Foundation-funded project to determine the biological function of each protein in plants.Sun Q, Zybailov B, Majeran W, Friso G, Olinares PD, van Wijk KJ.Atomic mass: right |thumb|200px|Stylized [[lithium-7 atom: 3 protons, 4 neutrons, & 3 electrons (total electrons are ~1/4300th of the mass of the nucleus). It has a mass of 7.Tandem mass spectrometry: 300 px|right|thumb|A [[Quadrupole mass analyzer|quadrupole time-of-flight hybrid tandem mass spectrometer.]]Two-dimensional gel electrophoresisHuman Proteinpedia: Human Proteinpedia is a portal for sharing and integration of human proteomic data,.Kandasamy et al.Isotope-coded affinity tag: An Isotope-coded affinity tag (ICAT) is an isotopic labeling method used for quantitative proteomics by mass spectrometry that uses chemical labeling reagents. "Rapid quantitative analysis of proteins or protein function in complex mixtures," Rudolf Hans Aebersold et al.Soft laser desorption: Soft laser desorption is laser desorption of large molecules that results in ionization without fragmentation. "Soft" in the context of ion formation means forming ions without breaking chemical bonds.Lattice protein: Lattice proteins are highly simplified computer models of proteins which are used to investigate protein folding.Peptide microarray: A peptide microarray (also commonly known as peptide chip or peptide epitope microarray) is a collection of peptides displayed on a solid surface, usually a glass or plastic chip. Peptide chips are used by scientists in biology, medicine and pharmacology to study binding properties and functionality and kinetics of protein-protein interactions in general.PSI Protein Classifier: PSI Protein Classifier is a program generalizing the results of both successive and independent iterations of the PSI-BLAST program. PSI Protein Classifier determines belonging of the found by PSI-BLAST proteins to the known families.SEA Native Peptide LigationMac OS X Server 1.0Protein subcellular localization prediction: Protein subcellular localization prediction (or just protein localization prediction) involves the computational prediction of where a protein resides in a cell.Protein primary structure: The primary structure of a peptide or protein is the linear sequence of its amino acid structural units, and partly comprises its overall biomolecular structure. By convention, the primary structure of a protein is reported starting from the amino-terminal (N) end to the carboxyl-terminal (C) end.Generalizability theory: Generalizability theory, or G Theory, is a statistical framework for conceptualizing, investigating, and designing reliable observations. It is used to determine the reliability (i.Coles PhillipsOntario Genomics Institute: The Ontario Genomics Institute (OGI) is a not-for-profit organization that manages cutting-edge genomics research projects and platforms.The Ontario Genomics Institute OGI also helps scientists find paths to the marketplace for their discoveries and the products to which they lead, and it works through diverse outreach and educational activities to raise awareness and facilitate informed public dialogue about genomics and its social impacts.Clonal Selection Algorithm: In artificial immune systems, Clonal selection algorithms are a class of algorithms inspired by the clonal selection theory of acquired immunity that explains how B and T lymphocytes improve their response to antigens over time called affinity maturation. These algorithms focus on the Darwinian attributes of the theory where selection is inspired by the affinity of antigen-antibody interactions, reproduction is inspired by cell division, and variation is inspired by somatic hypermutation.KIAA0895L: Uncharacterized protein KIAA0895-like also known as LOC653319, is a protein that in humans is encoded by the KIAA0895L gene.Gene signature: A gene signature is a group of genes in a cell whose combined expression patternItadani H, Mizuarai S, Kotani H. Can systems biology understand pathway activation?Biomarkers of aging: Biomarkers of aging are biomarkers that better predict functional capacity at a later age than chronological age. Stated another way, biomarkers of aging would give the true "biological age", which may be different from the chronological age.Electrospray ionizationDifference gel electrophoresis: Difference gel electrophoresis (DIGE) is a form of gel electrophoresis where up to three different protein samples can be labeled with size-matched, charge-matched spectrally resolvable fluorescent dyes (for example Cy3, Cy5, Cy2) prior to two-dimensional electrophoresis.Unlü M, Morgan ME, Minden JS.Protein–protein interactionMetabolomics: Metabolomics is the scientific study of chemical processes involving metabolites. Specifically, metabolomics is the "systematic study of the unique chemical fingerprints that specific cellular processes leave behind", the study of their small-molecule metabolite profiles.List of systems biology conferences: Systems biology is a biological study field that focuses on the systematic study of complex interactions in biological systems, thus using a new perspective (integration instead of reduction) to study them. Particularly from year 2000 onwards, the term is used widely in the biosciences.Flux (metabolism): Flux, or metabolic flux is the rate of turnover of molecules through a metabolic pathway. Flux is regulated by the enzymes involved in a pathway.SciDBBlood proteins: Plasma proteins, also termed serum proteins or blood proteins, are proteins present in blood plasma. They serve many different functions, including transport of lipids, hormones, vitamins and minerals in the circulatory system and the regulation of acellular activity and functioning of the immune system.THC-O-phosphateImmersive technologyArk (search engine): Ark is a personal search engine that uses filters such as hometown, current city, high school, college, gender, relationship status, employee, and interests, to search for new people, old classmates, old friends or acquaintances, and new business contacts. Features include managing users' inboxes from their mobile devices, and syncing data from their Yahoo, Aol, Gmail or Google Apps email accounts, while also finding information about whom they are communicating with.Internet organizations: This is a list of Internet organizations, or organizations that play or played a key role in the evolution of the Internet by developing recommendations, standards, and technology; deploying infrastructure and services; and addressing other major issues.Cancer biomarkers: A cancer biomarker refers to a substance or process that is indicative of the presence of cancer in the body. A biomarker may be a molecule secreted by a tumor or a specific response of the body to the presence of cancer.Potash: Potash is any of various mined and manufactured salts that contain potassium in water-soluble form.Potash, USGS 2008 Minerals Yearbook The name derives from "pot ash", which refers to plant ashes soaked in water in a pot, the primary means of manufacturing the product before the industrial era.Ferric uptake regulator family: In molecular biology, the ferric uptake regulator (FUR) family of proteins includes metal ion uptake regulator proteins. These are responsible for controlling the intracellular concentration of iron in many bacteria.Matrix model: == Mathematics and physics ==

(1/9620) Identification and characterization of subfamily-specific signatures in a large protein superfamily by a hidden Markov model approach.

BACKGROUND: Most profile and motif databases strive to classify protein sequences into a broad spectrum of protein families. The next step of such database studies should include the development of classification systems capable of distinguishing between subfamilies within a structurally and functionally diverse superfamily. This would be helpful in elucidating sequence-structure-function relationships of proteins. RESULTS: Here, we present a method to diagnose sequences into subfamilies by employing hidden Markov models (HMMs) to find windows of residues that are distinct among subfamilies (called signatures). The method starts with a multiple sequence alignment (MSA) of the subfamily. Then, we build a HMM database representing all sliding windows of the MSA of a fixed size. Finally, we construct a HMM histogram of the matches of each sliding window in the entire superfamily. To illustrate the efficacy of the method, we have applied the analysis to find subfamily signatures in two well-studied superfamilies: the cadherin and the EF-hand protein superfamilies. As a corollary, the HMM histograms of the analyzed subfamilies revealed information about their Ca2+ binding sites and loops. CONCLUSIONS: The method is used to create HMM databases to diagnose subfamilies of protein superfamilies that complement broad profile and motif databases such as BLOCKS, PROSITE, Pfam, SMART, PRINTS and InterPro.  (+info)

(2/9620) Protein interactions: two methods for assessment of the reliability of high throughput observations.

High throughput methods for detecting protein interactions require assessment of their accuracy. We present two forms of computational assessment. The first method is the expression profile reliability (EPR) index. The EPR index estimates the biologically relevant fraction of protein interactions detected in a high throughput screen. It does so by comparing the RNA expression profiles for the proteins whose interactions are found in the screen with expression profiles for known interacting and non-interacting pairs of proteins. The second form of assessment is the paralogous verification method (PVM). This method judges an interaction likely if the putatively interacting pair has paralogs that also interact. In contrast to the EPR index, which evaluates datasets of interactions, PVM scores individual interactions. On a test set, PVM identifies correctly 40% of true interactions with a false positive rate of approximately 1%. EPR and PVM were applied to the Database of Interacting Proteins (DIP), a large and diverse collection of protein-protein interactions that contains over 8000 Saccharomyces cerevisiae pairwise protein interactions. Using these two methods, we estimate that approximately 50% of them are reliable, and with the aid of PVM we identify confidently 3003 of them. Web servers for both the PVM and EPR methods are available on the DIP website (dip.doe-mbi.ucla.edu/Services.cgi).  (+info)

(3/9620) Stable isotope labeling by amino acids in cell culture, SILAC, as a simple and accurate approach to expression proteomics.

Quantitative proteomics has traditionally been performed by two-dimensional gel electrophoresis, but recently, mass spectrometric methods based on stable isotope quantitation have shown great promise for the simultaneous and automated identification and quantitation of complex protein mixtures. Here we describe a method, termed SILAC, for stable isotope labeling by amino acids in cell culture, for the in vivo incorporation of specific amino acids into all mammalian proteins. Mammalian cell lines are grown in media lacking a standard essential amino acid but supplemented with a non-radioactive, isotopically labeled form of that amino acid, in this case deuterated leucine (Leu-d3). We find that growth of cells maintained in these media is no different from growth in normal media as evidenced by cell morphology, doubling time, and ability to differentiate. Complete incorporation of Leu-d3 occurred after five doublings in the cell lines and proteins studied. Protein populations from experimental and control samples are mixed directly after harvesting, and mass spectrometric identification is straightforward as every leucine-containing peptide incorporates either all normal leucine or all Leu-d3. We have applied this technique to the relative quantitation of changes in protein expression during the process of muscle cell differentiation. Proteins that were found to be up-regulated during this process include glyceraldehyde-3-phosphate dehydrogenase, fibronectin, and pyruvate kinase M2. SILAC is a simple, inexpensive, and accurate procedure that can be used as a quantitative proteomic approach in any cell culture system.  (+info)

(4/9620) Biophysical characterization of proteins in the post-genomic era of proteomics.

Proteomics focuses on the high throughput study of the expression, structure, interactions, and, to some extent, function of large numbers of proteins. A true understanding of the functioning of a living cell also requires a quantitative description of the stoichiometry, kinetics, and energetics of each protein complex in a cellular pathway. Classical molecular biophysical studies contribute to understanding of these detailed properties of proteins on a smaller scale than does proteomics in that individual proteins are usually studied. This perspective article deals with the role of biophysical methods in the study of proteins in the proteomic era. Several important physical biochemical methods are discussed briefly and critiqued from the standpoint of information content and data acquisition. The focus is on conformational changes and macromolecular assembly, the utility of dynamic and static structural data, and the necessity to combine experimental approaches to obtain a full functional description. The conclusions are that biophysical information on proteins is a useful adjunct to "standard" proteomic methods, that data can be obtained by high throughput technology in some instances, but that hypothesis-driven experimentation may frequently be required.  (+info)

(5/9620) A proteomic analysis of human cilia: identification of novel components.

Cilia play an essential role in protecting the respiratory tract by providing the force necessary for mucociliary clearance. Although the major structural components of human cilia have been described, a complete understanding of cilia function and regulation will require identification and characterization of all ciliary components. Estimates from studies of Chlamydomonas flagella predict that an axoneme contains > or = 250 proteins. To identify all the components of human cilia, we have begun a comprehensive proteomic analysis of isolated ciliary axonemes. Analysis by two-dimensional (2-D) PAGE resulted in a highly reproducible 2-D map consisting of over 240 well resolved components. Individual protein spots were digested with trypsin and sequenced using liquid chromatography/tandem mass spectrometry (LC/MS/MS). Peptide matches were obtained to 38 potential ciliary proteins by this approach. To identify ciliary components not resolved by 2-D PAGE, axonemal proteins were separated on a one-dimensional gel. The gel lane was divided into 45 individual slices, each of which was analyzed by LC/MS/MS. This experiment resulted in peptide matches to an additional 110 proteins. In a third approach, preparations of isolated axonemes were digested with Lys-C, and the resulting peptides were analyzed directly by LC/MS/MS or by multidimensional LC/MS/MS, leading to the identification of a further 66 proteins. Each of the four approaches resulted in the identification of a subset of the proteins present. In total, sequence data were obtained on over 1400 peptides, and over 200 potential axonemal proteins were identified. Peptide matches were also obtained to over 200 human expressed sequence tags. As an approach to validate the mass spectrometry results, additional studies examined the expression of several identified proteins (annexin I, sperm protein Sp17, retinitis pigmentosa protein RP1) in cilia or ciliated cells. These studies represent the first proteomic analysis of the human ciliary axoneme and have identified many potentially novel components of this complex organelle.  (+info)

(6/9620) A proteomics approach for the identification of DNA binding activities observed in the electrophoretic mobility shift assay.

Transcription factors lie at the center of gene regulation, and their identification is crucial to the understanding of transcription and gene expression. Traditionally, the isolation and identification of transcription factors has been a long and laborious task. We present here a novel method for the identification of DNA-binding proteins seen in electrophoretic mobility shift assay (EMSA) using the power of two-dimensional electrophoresis coupled with mass spectrometry. By coupling SDS-PAGE and isoelectric focusing to EMSA, the molecular mass and pI of a protein complex seen in EMSA were estimated. Candidate proteins were then identified on a two-dimensional array at the predetermined pI and molecular mass coordinates and identified by mass spectrometry. We show here the successful isolation of a functionally relevant transcription factor and validate the identity through EMSA supershift analysis.  (+info)

(7/9620) A proteomics approach to identify proliferating cell nuclear antigen (PCNA)-binding proteins in human cell lysates. Identification of the human CHL12/RFCs2-5 complex as a novel PCNA-binding protein.

Proliferating cell nuclear antigen (PCNA), a eukaryotic DNA replication factor, functions not only as a processivity factor for DNA polymerase delta but also as a binding partner for multiple other factors. To understand its broad significance, we have carried out systematic studies of PCNA-binding proteins by a combination of affinity chromatography and mass spectrometric analyses. We detected more than 20 specific protein bands of various intensities in fractions bound to PCNA-fixed resin from human cell lysates and determined their peptide sequences by liquid chromatography and tandem mass spectrometry. A search with human protein data bases identified 12 reported PCNA-binding proteins from both cytoplasmic (S100 lysate) and nuclear extracts with substantial significance and four more solely from the nuclear preparation. CHL12, a factor involved in checkpoint response and chromosome cohesion, was a novel example found in both lysates. Further studies with recombinant proteins demonstrated that CHL12 and small subunits of replication factor C form a complex that interacts with PCNA.  (+info)

(8/9620) Peptidomics of the larval Drosophila melanogaster central nervous system.

Neuropeptides regulate most, if not all, biological processes in the animal kingdom, but only seven have been isolated and sequenced from Drosophila melanogaster. In analogy with the proteomics technology, where all proteins expressed in a cell or tissue are analyzed, the peptidomics approach aims at the simultaneous identification of the whole peptidome of a cell or tissue, i.e. all expressed peptides with their posttranslational modifications. Using nanoscale liquid chromatography combined with tandem mass spectrometry and data base mining, we analyzed the peptidome of the larval Drosophila central nervous system at the amino acid sequence level. We were able to provide biochemical evidence for the presence of 28 neuropeptides using an extract of only 50 larval Drosophila central nervous systems. Eighteen of these peptides are encoded in previously cloned or annotated precursor genes, although not all of them were predicted correctly. Eleven of these peptides were never purified before. Eight other peptides are entirely novel and are encoded in five different, not yet annotated genes. This neuropeptide expression profiling study also opens perspectives for other eukaryotic model systems, for which genome projects are completed or in progress.  (+info)


Quantitative


  • Quantitative proteomics analysis demonstrates post-transcriptional regulation of embryonic stem cell differentiation to hematopoiesis. (openrepository.com)


Analyses


  • Proteomics analyses the protein molecules produced by our genes. (bbc.co.uk)
  • Real-time, western blotting, and immunohistochemistry analyses were performed to validate the proteomics results. (bmj.com)


Proteins


  • In the past we've only been able to look at a handful of proteins at the same time, but using proteomics, we can examine a tissue sample for hundreds, possibly even thousands, of proteins at once. (bbc.co.uk)


Bioinformatics


  • This course is a survey of modern techniques of protein biochemistry, bioinformatics, proteomics, and functional genomics. (rutgers.edu)
  • 1. Students should demonstrate an understanding of the knowledge that is needed to begin biomedical research in the areas of proteomics, functional genomics, and/or bioinformatics. (rutgers.edu)


molecular


  • In this review novel findings obtained with adipo-proteomics studies are highlighted and the relevance of proteomics technologies to further understand molecular aspects of adipocyte biology is discussed. (rsc.org)


research


  • Since the postgenomic era began, proteomics has become the first choice among research approaches available for environmental adaptation and stress response investigators. (wiley.com)
  • Is the word 'biomarker' being properly used by proteomics research in neuroscience? (mpg.de)
  • This presentation will provide an overview of the recent applications of proteomics analysis techniques in the field of glaucoma research and will highlight valuable implications in translational and clinical research towards better understanding and treatment of glaucomatous neurodegeneration. (arvojournals.org)


significantly


  • Independent proteomics studies have contributed significantly to further insight into adipocyte biology, particularly with respect to adipokine profiling. (rsc.org)


Applications


  • The latest developments in the applications of proteomics to understand physiological changes in Lactobacillus species under harsh conditions are remarkable. (wiley.com)


techniques


  • Background Recent works by our group provide evidences of the usefulness of proteomics techniques for anti-osteoarthritis (OA) drug screening [ 1 - 3 ]. (bmj.com)


University


  • Researchers from the University of Westminster and the Karolinska Institute in Sweden used proteomics to analyse 32 samples of breast tissue to see if a standard technique could be developed to pick out breast cancers from benign lumps. (bbc.co.uk)


article


  • Is the Subject Area "Proteomics" applicable to this article? (plos.org)


The scientific field devoted to developing new drugs for treating genetic disorders is?


  • A. bioinformatics. B. proteomics. C. genetic profiling. D. genomics.
  • It's D- Genomics.


Why do the fields of proteomics and bioinformatics depends on genomics.?


  • Why do the fields of proteomics and bioinformatics depends on genomics.?
  • Proteomics is the study of protein action. It depends on genomics because proteins are build based on genes. Bioinformatics is not dependent on either, they are dependent on bioinformatics. Bioinformatics is the computational field involved with the aggregation and analysis of genomic and proteomics data.


Can someone explain the issue of drinking alcohol and taking drugs?


  • Please could you write them in separate paragraphs. It must be about drugs and alcohol in the UK. Please be as detailed as you can. Explain why each are public health issues/problems. And what evidence there is to support this. This is for part of my coursework, so I will need as much information as possible. And if you have any good websites or links, please post them. Thank you.
  • I'm sorry this drug link rather sucks, it's basically rhetoric for kids. If I find a better one I'll edit my post. http://kidshealth.org/kid/grow/drugs_alcohol/know_drugs.html Chronic alcohol abuse disrupts the proteins that keep fluids out of the lung, lowers a protective antioxidant, disrupts immune defenses and can lead to a condition known as 'alcoholic lung,' according to research to be presented at the conference, "Physiological Genomics and Proteomics of Lung Disease." The findings give insight into how excessive drinking can harm the molecular life of the lung and lead to serious illness, including pneumonia and acute respiratory distress syndrome (ARDS). (there's more at the link. this was just a sample) http://www.medicalnewstoday.com/articles/55970.php Yay! Found a better one. There's 8 pages and it looks pretty good. http://yourtotalhealth.ivillage.com/illegal-drugs-heart.html


why do we use heat in carbohydrates test but not protein test?


  • why do we use heat in carbohydrates test but not protein test?
  • Heat warps the shape of the proteins & can denature them, it supplies more energy to the bonds which could either change the molecules shape by allowing certain bonds to occur that wouldn't occur at room temperature or break it. An example of such would be when an egg is cracked into a frying pan, the white part is proteins changing formation from the heat supplied. I'm not particularly sure as to which however as i'm not particularly versed in Proteomics .


What do they check for in a blood test when they find lumps in your breast?


  • my doctor took blood and is supposed to call me today sometime with the results but I don't remember what he said he was looking for. all I remember was something about lymphoma and red blood cells. Does anyone know?
  • . Laboratory Findings A consistently elevated sedimentation rate may be the result of disseminated cancer. Liver or bone metastases may be associated with elevation of serum alkaline phosphatase. Hypercalcemia is an occasional important finding in advanced cancer of the breast. Carcinoembryonic antigen (CEA) and CA 15-3 or CA 27-29 may be used as markers for recurrent breast cancer but are not helpful in diagnosing early lesions. Many scientists are further investigating breast cancer biomarkers through proteomics and hormone assays. These studies are ongoing and may prove to be helpful in early detection or evaluation of prognosis.


What is piperlongumine ;it might be used to treat cancer?



Can somebody smart with a background in Melanoma or Cancer please translate this?


  • Our laboratory's research interest is focused on the molecular genetics of melanoma with a particular focus on the discovery on genetic alterations useful for diagnosis, classification, and therapy. Using genome-wide DNA copy and mutation analyses and by correlating them with clinical and histomorphologic features we have identified distinct subtypes of melanoma. These findings have become clinically relevant, as the subtypes are enriched in specific mutations such as in KIT and BRAF, for which specific inhibitors are already approved or under advanced clinical investigation. We believe that an improved disease classification with the precise delineation of biologically distinct, molecularly defined subtypes is the key to improving prevention, diagnosis, and therapy of melanoma. More recently, we have identified mutations in the heterotrimeric G-protein GNAQ in uveal melanoma and in melanocytic tumors that arise in the dermis. Our current research interest is focused on understanding the signaling downstream of these mutations to identify targets for therapeutic intervention. We are pursuing a multi-pronged approach using genomics, proteomics, high-throughput RNAi and drug screens and are developing preclinical models to help accelerate the clinical translation of these findings. In addition, we are developing molecular assays to assist in the distinction of malignant and benign melanocytic tumors. We have found that comparative genomic hybridization and fluorescence in situ hybridization can assist in classifying tumors that have equivocal histopathologic features and cannot be reliably diagnosed as benign or malignant with current methods.
  • What this lab is trying to do is find is tumor markers and genetic mutations to help in the classification and staging of melanoma which will also help in the diagnosis of a particular types of melanoma and directed treatment of those types.


would a blood test detect cancer somewhere in the body or cancerous cells?



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