Polymorphism, Restriction Fragment Length: Variation occurring within a species in the presence or length of DNA fragment generated by a specific endonuclease at a specific site in the genome. Such variations are generated by mutations that create or abolish recognition sites for these enzymes or change the length of the fragment.Polymorphism, Genetic: The regular and simultaneous occurrence in a single interbreeding population of two or more discontinuous genotypes. The concept includes differences in genotypes ranging in size from a single nucleotide site (POLYMORPHISM, SINGLE NUCLEOTIDE) to large nucleotide sequences visible at a chromosomal level.Polymerase Chain Reaction: In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.Polymorphism, Single Nucleotide: A single nucleotide variation in a genetic sequence that occurs at appreciable frequency in the population.DNA Restriction Enzymes: Enzymes that are part of the restriction-modification systems. They catalyze the endonucleolytic cleavage of DNA sequences which lack the species-specific methylation pattern in the host cell's DNA. Cleavage yields random or specific double-stranded fragments with terminal 5'-phosphates. The function of restriction enzymes is to destroy any foreign DNA that invades the host cell. Most have been studied in bacterial systems, but a few have been found in eukaryotic organisms. They are also used as tools for the systematic dissection and mapping of chromosomes, in the determination of base sequences of DNAs, and have made it possible to splice and recombine genes from one organism into the genome of another. EC 3.21.1.DNA, Bacterial: Deoxyribonucleic acid that makes up the genetic material of bacteria.Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.Genotype: The genetic constitution of the individual, comprising the ALLELES present at each GENETIC LOCUS.DNA Fingerprinting: A technique for identifying individuals of a species that is based on the uniqueness of their DNA sequence. Uniqueness is determined by identifying which combination of allelic variations occur in the individual at a statistically relevant number of different loci. In forensic studies, RESTRICTION FRAGMENT LENGTH POLYMORPHISM of multiple, highly polymorphic VNTR LOCI or MICROSATELLITE REPEAT loci are analyzed. The number of loci used for the profile depends on the ALLELE FREQUENCY in the population.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Alleles: Variant forms of the same gene, occupying the same locus on homologous CHROMOSOMES, and governing the variants in production of the same gene product.Amplified Fragment Length Polymorphism Analysis: The detection of RESTRICTION FRAGMENT LENGTH POLYMORPHISMS by selective PCR amplification of restriction fragments derived from genomic DNA followed by electrophoretic analysis of the amplified restriction fragments.Deoxyribonucleases, Type II Site-Specific: Enzyme systems containing a single subunit and requiring only magnesium for endonucleolytic activity. The corresponding modification methylases are separate enzymes. The systems recognize specific short DNA sequences and cleave either within, or at a short specific distance from, the recognition sequence to give specific double-stranded fragments with terminal 5'-phosphates. Enzymes from different microorganisms with the same specificity are called isoschizomers. EC 3.1.21.4.Gene Frequency: The proportion of one particular in the total of all ALLELES for one genetic locus in a breeding POPULATION.Bacterial Typing Techniques: Procedures for identifying types and strains of bacteria. The most frequently employed typing systems are BACTERIOPHAGE TYPING and SEROTYPING as well as bacteriocin typing and biotyping.Sequence Analysis, DNA: A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.DNA, Ribosomal: DNA sequences encoding RIBOSOMAL RNA and the segments of DNA separating the individual ribosomal RNA genes, referred to as RIBOSOMAL SPACER DNA.Chromosome Mapping: Any method used for determining the location of and relative distances between genes on a chromosome.Genetic Variation: Genotypic differences observed among individuals in a population.Phylogeny: The relationships of groups of organisms as reflected by their genetic makeup.Blotting, Southern: A method (first developed by E.M. Southern) for detection of DNA that has been electrophoretically separated and immobilized by blotting on nitrocellulose or other type of paper or nylon membrane followed by hybridization with labeled NUCLEIC ACID PROBES.Nucleic Acid Hybridization: Widely used technique which exploits the ability of complementary sequences in single-stranded DNAs or RNAs to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (Kendrew, Encyclopedia of Molecular Biology, 1994, p503)RNA, Ribosomal, 16S: Constituent of 30S subunit prokaryotic ribosomes containing 1600 nucleotides and 21 proteins. 16S rRNA is involved in initiation of polypeptide synthesis.Genetic Predisposition to Disease: A latent susceptibility to disease at the genetic level, which may be activated under certain conditions.Genetic Markers: A phenotypically recognizable genetic trait which can be used to identify a genetic locus, a linkage group, or a recombination event.DNA: A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).DNA Probes: Species- or subspecies-specific DNA (including COMPLEMENTARY DNA; conserved genes, whole chromosomes, or whole genomes) used in hybridization studies in order to identify microorganisms, to measure DNA-DNA homologies, to group subspecies, etc. The DNA probe hybridizes with a specific mRNA, if present. Conventional techniques used for testing for the hybridization product include dot blot assays, Southern blot assays, and DNA:RNA hybrid-specific antibody tests. Conventional labels for the DNA probe include the radioisotope labels 32P and 125I and the chemical label biotin. The use of DNA probes provides a specific, sensitive, rapid, and inexpensive replacement for cell culture techniques for diagnosing infections.Genetic Linkage: The co-inheritance of two or more non-allelic GENES due to their being located more or less closely on the same CHROMOSOME.DNA Transposable Elements: Discrete segments of DNA which can excise and reintegrate to another site in the genome. Most are inactive, i.e., have not been found to exist outside the integrated state. DNA transposable elements include bacterial IS (insertion sequence) elements, Tn elements, the maize controlling elements Ac and Ds, Drosophila P, gypsy, and pogo elements, the human Tigger elements and the Tc and mariner elements which are found throughout the animal kingdom.Cloning, Molecular: The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.Restriction Mapping: Use of restriction endonucleases to analyze and generate a physical map of genomes, genes, or other segments of DNA.DNA Primers: Short sequences (generally about 10 base pairs) of DNA that are complementary to sequences of messenger RNA and allow reverse transcriptases to start copying the adjacent sequences of mRNA. Primers are used extensively in genetic and molecular biology techniques.Haplotypes: The genetic constitution of individuals with respect to one member of a pair of allelic genes, or sets of genes that are closely linked and tend to be inherited together such as those of the MAJOR HISTOCOMPATIBILITY COMPLEX.Deoxyribonuclease HindIII: One of the Type II site-specific deoxyribonucleases (EC 3.1.21.4). It recognizes and cleaves the sequence A/AGCTT at the slash. HindIII is from Haemophilus influenzae R(d). Numerous isoschizomers have been identified. EC 3.1.21.-.Molecular Epidemiology: The application of molecular biology to the answering of epidemiological questions. The examination of patterns of changes in DNA to implicate particular carcinogens and the use of molecular markers to predict which individuals are at highest risk for a disease are common examples.Genes, Bacterial: The functional hereditary units of BACTERIA.Species Specificity: The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.Deoxyribonuclease EcoRI: One of the Type II site-specific deoxyribonucleases (EC 3.1.21.4). It recognizes and cleaves the sequence G/AATTC at the slash. EcoRI is from E coliRY13. Several isoschizomers have been identified. EC 3.1.21.-.DNA, Ribosomal Spacer: The intergenic DNA segments that are between the ribosomal RNA genes (internal transcribed spacers) and between the tandemly repeated units of rDNA (external transcribed spacers and nontranscribed spacers).Case-Control Studies: Studies which start with the identification of persons with a disease of interest and a control (comparison, referent) group without the disease. The relationship of an attribute to the disease is examined by comparing diseased and non-diseased persons with regard to the frequency or levels of the attribute in each group.Deoxyribonuclease HpaII: One of the Type II site-specific deoxyribonucleases (EC 3.1.21.4). It recognizes and cleaves the sequences C/CGG and GGC/C at the slash. HpaII is from Haemophilus parainfluenzae. Several isoschizomers have been identified. EC 3.1.21.-.Peptide Fragments: Partial proteins formed by partial hydrolysis of complete proteins or generated through PROTEIN ENGINEERING techniques.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Mutation: Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.Minisatellite Repeats: Tandem arrays of moderately repetitive, short (10-60 bases) DNA sequences which are found dispersed throughout the GENOME, at the ends of chromosomes (TELOMERES), and clustered near telomeres. Their degree of repetition is two to several hundred at each locus. Loci number in the thousands but each locus shows a distinctive repeat unit.Mycobacterium tuberculosis: A species of gram-positive, aerobic bacteria that produces TUBERCULOSIS in humans, other primates, CATTLE; DOGS; and some other animals which have contact with humans. Growth tends to be in serpentine, cordlike masses in which the bacilli show a parallel orientation.Genes: A category of nucleic acid sequences that function as units of heredity and which code for the basic instructions for the development, reproduction, and maintenance of organisms.Electrophoresis, Gel, Pulsed-Field: Gel electrophoresis in which the direction of the electric field is changed periodically. This technique is similar to other electrophoretic methods normally used to separate double-stranded DNA molecules ranging in size up to tens of thousands of base-pairs. However, by alternating the electric field direction one is able to separate DNA molecules up to several million base-pairs in length.Bacteria: One of the three domains of life (the others being Eukarya and ARCHAEA), also called Eubacteria. They are unicellular prokaryotic microorganisms which generally possess rigid cell walls, multiply by cell division, and exhibit three principal forms: round or coccal, rodlike or bacillary, and spiral or spirochetal. Bacteria can be classified by their response to OXYGEN: aerobic, anaerobic, or facultatively anaerobic; by the mode by which they obtain their energy: chemotrophy (via chemical reaction) or PHOTOTROPHY (via light reaction); for chemotrophs by their source of chemical energy: CHEMOLITHOTROPHY (from inorganic compounds) or chemoorganotrophy (from organic compounds); and by their source for CARBON; NITROGEN; etc.; HETEROTROPHY (from organic sources) or AUTOTROPHY (from CARBON DIOXIDE). They can also be classified by whether or not they stain (based on the structure of their CELL WALLS) with CRYSTAL VIOLET dye: gram-negative or gram-positive.Asian Continental Ancestry Group: Individuals whose ancestral origins are in the southeastern and eastern areas of the Asian continent.Electrophoresis, Agar Gel: Electrophoresis in which agar or agarose gel is used as the diffusion medium.Caloric Restriction: Reduction in caloric intake without reduction in adequate nutrition. In experimental animals, caloric restriction has been shown to extend lifespan and enhance other physiological variables.Repetitive Sequences, Nucleic Acid: Sequences of DNA or RNA that occur in multiple copies. There are several types: INTERSPERSED REPETITIVE SEQUENCES are copies of transposable elements (DNA TRANSPOSABLE ELEMENTS or RETROELEMENTS) dispersed throughout the genome. TERMINAL REPEAT SEQUENCES flank both ends of another sequence, for example, the long terminal repeats (LTRs) on RETROVIRUSES. Variations may be direct repeats, those occurring in the same direction, or inverted repeats, those opposite to each other in direction. TANDEM REPEAT SEQUENCES are copies which lie adjacent to each other, direct or inverted (INVERTED REPEAT SEQUENCES).Plasmids: Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.Phenotype: The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment.Sequence Homology, Nucleic Acid: The sequential correspondence of nucleotides in one nucleic acid molecule with those of another nucleic acid molecule. Sequence homology is an indication of the genetic relatedness of different organisms and gene function.Pedigree: The record of descent or ancestry, particularly of a particular condition or trait, indicating individual family members, their relationships, and their status with respect to the trait or condition.DNA, Fungal: Deoxyribonucleic acid that makes up the genetic material of fungi.Soil Microbiology: The presence of bacteria, viruses, and fungi in the soil. This term is not restricted to pathogenic organisms.Heterozygote: An individual having different alleles at one or more loci regarding a specific character.Serotyping: Process of determining and distinguishing species of bacteria or viruses based on antigens they share.Heterozygote Detection: Identification of genetic carriers for a given trait.Cluster Analysis: A set of statistical methods used to group variables or observations into strongly inter-related subgroups. In epidemiology, it may be used to analyze a closely grouped series of events or cases of disease or other health-related phenomenon with well-defined distribution patterns in relation to time or place or both.Polymorphism, Single-Stranded Conformational: Variation in a population's DNA sequence that is detected by determining alterations in the conformation of denatured DNA fragments. Denatured DNA fragments are allowed to renature under conditions that prevent the formation of double-stranded DNA and allow secondary structure to form in single stranded fragments. These fragments are then run through polyacrylamide gels to detect variations in the secondary structure that is manifested as an alteration in migration through the gels.Genetic Association Studies: The analysis of a sequence such as a region of a chromosome, a haplotype, a gene, or an allele for its involvement in controlling the phenotype of a specific trait, metabolic pathway, or disease.China: A country spanning from central Asia to the Pacific Ocean.DNA, Viral: Deoxyribonucleic acid that makes up the genetic material of viruses.RNA, Bacterial: Ribonucleic acid in bacteria having regulatory and catalytic roles as well as involvement in protein synthesis.Homozygote: An individual in which both alleles at a given locus are identical.RNA, Ribosomal: The most abundant form of RNA. Together with proteins, it forms the ribosomes, playing a structural role and also a role in ribosomal binding of mRNA and tRNAs. Individual chains are conventionally designated by their sedimentation coefficients. In eukaryotes, four large chains exist, synthesized in the nucleolus and constituting about 50% of the ribosome. (Dorland, 28th ed)Deoxyribonuclease BamHI: One of the Type II site-specific deoxyribonucleases (EC 3.1.21.4). It recognizes and cleaves the sequence G/GATCC at the slash. BamHI is from Bacillus amyloliquefaciens N. Numerous isoschizomers have been identified. EC 3.1.21.-.Genes, rRNA: Genes, found in both prokaryotes and eukaryotes, which are transcribed to produce the RNA which is incorporated into RIBOSOMES. Prokaryotic rRNA genes are usually found in OPERONS dispersed throughout the GENOME, whereas eukaryotic rRNA genes are clustered, multicistronic transcriptional units.DNA, Protozoan: Deoxyribonucleic acid that makes up the genetic material of protozoa.Random Amplified Polymorphic DNA Technique: Technique that utilizes low-stringency polymerase chain reaction (PCR) amplification with single primers of arbitrary sequence to generate strain-specific arrays of anonymous DNA fragments. RAPD technique may be used to determine taxonomic identity, assess kinship relationships, analyze mixed genome samples, and create specific probes.Exons: The parts of a transcript of a split GENE remaining after the INTRONS are removed. They are spliced together to become a MESSENGER RNA or other functional RNA.JapanMycological Typing Techniques: Procedures for identifying types and strains of fungi.Ecosystem: A functional system which includes the organisms of a natural community together with their environment. (McGraw Hill Dictionary of Scientific and Technical Terms, 4th ed)Escherichia coli: A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.Bacterial Proteins: Proteins found in any species of bacterium.Linkage Disequilibrium: Nonrandom association of linked genes. This is the tendency of the alleles of two separate but already linked loci to be found together more frequently than would be expected by chance alone.Chromosome Deletion: Actual loss of portion of a chromosome.RNA, Ribosomal, 23S: Constituent of 50S subunit of prokaryotic ribosomes containing about 3200 nucleotides. 23S rRNA is involved in the initiation of polypeptide synthesis.DNA Mutational Analysis: Biochemical identification of mutational changes in a nucleotide sequence.Tuberculosis: Any of the infectious diseases of man and other animals caused by species of MYCOBACTERIUM.Recombination, Genetic: Production of new arrangements of DNA by various mechanisms such as assortment and segregation, CROSSING OVER; GENE CONVERSION; GENETIC TRANSFORMATION; GENETIC CONJUGATION; GENETIC TRANSDUCTION; or mixed infection of viruses.Disease Outbreaks: Sudden increase in the incidence of a disease. The concept includes EPIDEMICS and PANDEMICS.DNA, Mitochondrial: Double-stranded DNA of MITOCHONDRIA. In eukaryotes, the mitochondrial GENOME is circular and codes for ribosomal RNAs, transfer RNAs, and about 10 proteins.Codon: A set of three nucleotides in a protein coding sequence that specifies individual amino acids or a termination signal (CODON, TERMINATOR). Most codons are universal, but some organisms do not produce the transfer RNAs (RNA, TRANSFER) complementary to all codons. These codons are referred to as unassigned codons (CODONS, NONSENSE).Cattle: Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.Risk Factors: An aspect of personal behavior or lifestyle, environmental exposure, or inborn or inherited characteristic, which, on the basis of epidemiologic evidence, is known to be associated with a health-related condition considered important to prevent.X Chromosome: The female sex chromosome, being the differential sex chromosome carried by half the male gametes and all female gametes in human and other male-heterogametic species.Interspersed Repetitive Sequences: Copies of transposable elements interspersed throughout the genome, some of which are still active and often referred to as "jumping genes". There are two classes of interspersed repetitive elements. Class I elements (or RETROELEMENTS - such as retrotransposons, retroviruses, LONG INTERSPERSED NUCLEOTIDE ELEMENTS and SHORT INTERSPERSED NUCLEOTIDE ELEMENTS) transpose via reverse transcription of an RNA intermediate. Class II elements (or DNA TRANSPOSABLE ELEMENTS - such as transposons, Tn elements, insertion sequence elements and mobile gene cassettes of bacterial integrons) transpose directly from one site in the DNA to another.Promoter Regions, Genetic: DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.Crosses, Genetic: Deliberate breeding of two different individuals that results in offspring that carry part of the genetic material of each parent. The parent organisms must be genetically compatible and may be from different varieties or closely related species.DNA, Recombinant: Biologically active DNA which has been formed by the in vitro joining of segments of DNA from different sources. It includes the recombination joint or edge of a heteroduplex region where two recombining DNA molecules are connected.Molecular Typing: Using MOLECULAR BIOLOGY techniques, such as DNA SEQUENCE ANALYSIS; PULSED-FIELD GEL ELECTROPHORESIS; and DNA FINGERPRINTING, to identify, classify, and compare organisms and their subtypes.Point Mutation: A mutation caused by the substitution of one nucleotide for another. This results in the DNA molecule having a change in a single base pair.Multigene Family: A set of genes descended by duplication and variation from some ancestral gene. Such genes may be clustered together on the same chromosome or dispersed on different chromosomes. Examples of multigene families include those that encode the hemoglobins, immunoglobulins, histocompatibility antigens, actins, tubulins, keratins, collagens, heat shock proteins, salivary glue proteins, chorion proteins, cuticle proteins, yolk proteins, and phaseolins, as well as histones, ribosomal RNA, and transfer RNA genes. The latter three are examples of reiterated genes, where hundreds of identical genes are present in a tandem array. (King & Stanfield, A Dictionary of Genetics, 4th ed)Methylenetetrahydrofolate Reductase (NADPH2): A flavoprotein amine oxidoreductase that catalyzes the reversible conversion of 5-methyltetrahydrofolate to 5,10-methylenetetrahydrofolate. This enzyme was formerly classified as EC 1.1.1.171.Sequence Alignment: The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.Oligonucleotide Probes: Synthetic or natural oligonucleotides used in hybridization studies in order to identify and study specific nucleic acid fragments, e.g., DNA segments near or within a specific gene locus or gene. The probe hybridizes with a specific mRNA, if present. Conventional techniques used for testing for the hybridization product include dot blot assays, Southern blot assays, and DNA:RNA hybrid-specific antibody tests. Conventional labels for the probe include the radioisotope labels 32P and 125I and the chemical label biotin.Korea: Former kingdom, located on Korea Peninsula between Sea of Japan and Yellow Sea on east coast of Asia. In 1948, the kingdom ceased and two independent countries were formed, divided by the 38th parallel.Introns: Sequences of DNA in the genes that are located between the EXONS. They are transcribed along with the exons but are removed from the primary gene transcript by RNA SPLICING to leave mature RNA. Some introns code for separate genes.RNA, Ribosomal, 18S: Constituent of the 40S subunit of eukaryotic ribosomes. 18S rRNA is involved in the initiation of polypeptide synthesis in eukaryotes.Cosmids: Plasmids containing at least one cos (cohesive-end site) of PHAGE LAMBDA. They are used as cloning vehicles.Environmental Microbiology: The study of microorganisms living in a variety of environments (air, soil, water, etc.) and their pathogenic relationship to other organisms including man.DNA, Plant: Deoxyribonucleic acid that makes up the genetic material of plants.Biodiversity: The variety of all native living organisms and their various forms and interrelationships.Microsatellite Repeats: A variety of simple repeat sequences that are distributed throughout the GENOME. They are characterized by a short repeat unit of 2-8 basepairs that is repeated up to 100 times. They are also known as short tandem repeats (STRs).European Continental Ancestry Group: Individuals whose ancestral origins are in the continent of Europe.Hybrid Cells: Any cell, other than a ZYGOTE, that contains elements (such as NUCLEI and CYTOPLASM) from two or more different cells, usually produced by artificial CELL FUSION.Oligonucleotides: Polymers made up of a few (2-20) nucleotides. In molecular genetics, they refer to a short sequence synthesized to match a region where a mutation is known to occur, and then used as a probe (OLIGONUCLEOTIDE PROBES). (Dorland, 28th ed)Tuberculosis, Pulmonary: MYCOBACTERIUM infections of the lung.DNA, Neoplasm: DNA present in neoplastic tissue.Sensitivity and Specificity: Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed)Models, Genetic: Theoretical representations that simulate the behavior or activity of genetic processes or phenomena. They include the use of mathematical equations, computers, and other electronic equipment.Campylobacter jejuni: A species of bacteria that resemble small tightly coiled spirals. Its organisms are known to cause abortion in sheep and fever and enteritis in man and may be associated with enteric diseases of calves, lambs, and other animals.Mycobacterium: A genus of gram-positive, aerobic bacteria. Most species are free-living in soil and water, but the major habitat for some is the diseased tissue of warm-blooded hosts.Campylobacter Infections: Infections with bacteria of the genus CAMPYLOBACTER.Soil: The unconsolidated mineral or organic matter on the surface of the earth that serves as a natural medium for the growth of land plants.RNA, Messenger: RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.Length of Stay: The period of confinement of a patient to a hospital or other health facility.Transcription, Genetic: The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.Water Microbiology: The presence of bacteria, viruses, and fungi in water. This term is not restricted to pathogenic organisms.Ribotyping: RESTRICTION FRAGMENT LENGTH POLYMORPHISM analysis of rRNA genes that is used for differentiating between species or strains.IndiaElectrophoresis, Polyacrylamide Gel: Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.BrazilGene Amplification: A selective increase in the number of copies of a gene coding for a specific protein without a proportional increase in other genes. It occurs naturally via the excision of a copy of the repeating sequence from the chromosome and its extrachromosomal replication in a plasmid, or via the production of an RNA transcript of the entire repeating sequence of ribosomal RNA followed by the reverse transcription of the molecule to produce an additional copy of the original DNA sequence. Laboratory techniques have been introduced for inducing disproportional replication by unequal crossing over, uptake of DNA from lysed cells, or generation of extrachromosomal sequences from rolling circle replication.Plant Diseases: Diseases of plants.Immunoglobulin Fab Fragments: Univalent antigen-binding fragments composed of one entire IMMUNOGLOBULIN LIGHT CHAIN and the amino terminal end of one of the IMMUNOGLOBULIN HEAVY CHAINS from the hinge region, linked to each other by disulfide bonds. Fab contains the IMMUNOGLOBULIN VARIABLE REGIONS, which are part of the antigen-binding site, and the first IMMUNOGLOBULIN CONSTANT REGIONS. This fragment can be obtained by digestion of immunoglobulins with the proteolytic enzyme PAPAIN.TurkeyGenetics, Population: The discipline studying genetic composition of populations and effects of factors such as GENETIC SELECTION, population size, MUTATION, migration, and GENETIC DRIFT on the frequencies of various GENOTYPES and PHENOTYPES using a variety of GENETIC TECHNIQUES.Geologic Sediments: A mass of organic or inorganic solid fragmented material, or the solid fragment itself, that comes from the weathering of rock and is carried by, suspended in, or dropped by air, water, or ice. It refers also to a mass that is accumulated by any other natural agent and that forms in layers on the earth's surface, such as sand, gravel, silt, mud, fill, or loess. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed, p1689)Genes, Viral: The functional hereditary units of VIRUSES.Swine: Any of various animals that constitute the family Suidae and comprise stout-bodied, short-legged omnivorous mammals with thick skin, usually covered with coarse bristles, a rather long mobile snout, and small tail. Included are the genera Babyrousa, Phacochoerus (wart hogs), and Sus, the latter containing the domestic pig (see SUS SCROFA).Cell Line: Established cell cultures that have the potential to propagate indefinitely.Gene Library: A large collection of DNA fragments cloned (CLONING, MOLECULAR) from a given organism, tissue, organ, or cell type. It may contain complete genomic sequences (GENOMIC LIBRARY) or complementary DNA sequences, the latter being formed from messenger RNA and lacking intron sequences.PolandGenes, Plant: The functional hereditary units of PLANTS.Flagellin: A protein with a molecular weight of 40,000 isolated from bacterial flagella. At appropriate pH and salt concentration, three flagellin monomers can spontaneously reaggregate to form structures which appear identical to intact flagella.Evaluation Studies as Topic: Studies determining the effectiveness or value of processes, personnel, and equipment, or the material on conducting such studies. For drugs and devices, CLINICAL TRIALS AS TOPIC; DRUG EVALUATION; and DRUG EVALUATION, PRECLINICAL are available.Food Microbiology: The presence of bacteria, viruses, and fungi in food and food products. This term is not restricted to pathogenic organisms: the presence of various non-pathogenic bacteria and fungi in cheeses and wines, for example, is included in this concept.Drug Resistance, Microbial: The ability of microorganisms, especially bacteria, to resist or to become tolerant to chemotherapeutic agents, antimicrobial agents, or antibiotics. This resistance may be acquired through gene mutation or foreign DNA in transmissible plasmids (R FACTORS).Sequence Homology, Amino Acid: The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.Rickettsia: A genus of gram-negative, aerobic, rod-shaped bacteria often surrounded by a protein microcapsular layer and slime layer. The natural cycle of its organisms generally involves a vertebrate and an invertebrate host. Species of the genus are the etiological agents of human diseases, such as typhus.Molecular Weight: The sum of the weight of all the atoms in a molecule.Biota: The spectrum of different living organisms inhabiting a particular region, habitat, or biotope.Archaea: One of the three domains of life (the others being BACTERIA and Eukarya), formerly called Archaebacteria under the taxon Bacteria, but now considered separate and distinct. They are characterized by: (1) the presence of characteristic tRNAs and ribosomal RNAs; (2) the absence of peptidoglycan cell walls; (3) the presence of ether-linked lipids built from branched-chain subunits; and (4) their occurrence in unusual habitats. While archaea resemble bacteria in morphology and genomic organization, they resemble eukarya in their method of genomic replication. The domain contains at least four kingdoms: CRENARCHAEOTA; EURYARCHAEOTA; NANOARCHAEOTA; and KORARCHAEOTA.Virulence: The degree of pathogenicity within a group or species of microorganisms or viruses as indicated by case fatality rates and/or the ability of the organism to invade the tissues of the host. The pathogenic capacity of an organism is determined by its VIRULENCE FACTORS.Fresh Water: Water containing no significant amounts of salts, such as water from RIVERS and LAKES.DNA, Intergenic: Any of the DNA in between gene-coding DNA, including untranslated regions, 5' and 3' flanking regions, INTRONS, non-functional pseudogenes, and non-functional repetitive sequences. This DNA may or may not encode regulatory functions.Tunisia: A country in northern Africa between ALGERIA and LIBYA. Its capital is Tunis.Oryza sativa: Annual cereal grass of the family POACEAE and its edible starchy grain, rice, which is the staple food of roughly one-half of the world's population.Telomere: A terminal section of a chromosome which has a specialized structure and which is involved in chromosomal replication and stability. Its length is believed to be a few hundred base pairs.Evolution, Molecular: The process of cumulative change at the level of DNA; RNA; and PROTEINS, over successive generations.Glutathione Transferase: A transferase that catalyzes the addition of aliphatic, aromatic, or heterocyclic FREE RADICALS as well as EPOXIDES and arene oxides to GLUTATHIONE. Addition takes place at the SULFUR. It also catalyzes the reduction of polyol nitrate by glutathione to polyol and nitrite.Chromosomes, Human, 13-15: The medium-sized, acrocentric human chromosomes, called group D in the human chromosome classification. This group consists of chromosome pairs 13, 14, and 15.Tandem Repeat Sequences: Copies of DNA sequences which lie adjacent to each other in the same orientation (direct tandem repeats) or in the opposite direction to each other (INVERTED TANDEM REPEATS).Chromosome Banding: Staining of bands, or chromosome segments, allowing the precise identification of individual chromosomes or parts of chromosomes. Applications include the determination of chromosome rearrangements in malformation syndromes and cancer, the chemistry of chromosome segments, chromosome changes during evolution, and, in conjunction with cell hybridization studies, chromosome mapping.Pregnancy: The status during which female mammals carry their developing young (EMBRYOS or FETUSES) in utero before birth, beginning from FERTILIZATION to BIRTH.Karyotyping: Mapping of the KARYOTYPE of a cell.Chromosomes, Human, Pair 17: A specific pair of GROUP E CHROMOSOMES of the human chromosome classification.Chickens: Common name for the species Gallus gallus, the domestic fowl, in the family Phasianidae, order GALLIFORMES. It is descended from the red jungle fowl of SOUTHEAST ASIA.Odds Ratio: The ratio of two odds. The exposure-odds ratio for case control data is the ratio of the odds in favor of exposure among cases to the odds in favor of exposure among noncases. The disease-odds ratio for a cohort or cross section is the ratio of the odds in favor of disease among the exposed to the odds in favor of disease among the unexposed. The prevalence-odds ratio refers to an odds ratio derived cross-sectionally from studies of prevalent cases.Feces: Excrement from the INTESTINES, containing unabsorbed solids, waste products, secretions, and BACTERIA of the DIGESTIVE SYSTEM.Mycobacterium avium subsp. paratuberculosis: A subspecies of gram-positive, aerobic bacteria. It is the etiologic agent of Johne's disease (PARATUBERCULOSIS), a chronic GASTROENTERITIS in RUMINANTS.Mycobacterium avium: A bacterium causing tuberculosis in domestic fowl and other birds. In pigs, it may cause localized and sometimes disseminated disease. The organism occurs occasionally in sheep and cattle. It should be distinguished from the M. avium complex, which infects primarily humans.Base Composition: The relative amounts of the PURINES and PYRIMIDINES in a nucleic acid.Disease Susceptibility: A constitution or condition of the body which makes the tissues react in special ways to certain extrinsic stimuli and thus tends to make the individual more than usually susceptible to certain diseases.Reproducibility of Results: The statistical reproducibility of measurements (often in a clinical context), including the testing of instrumentation or techniques to obtain reproducible results. The concept includes reproducibility of physiological measurements, which may be used to develop rules to assess probability or prognosis, or response to a stimulus; reproducibility of occurrence of a condition; and reproducibility of experimental results.Genome, Bacterial: The genetic complement of a BACTERIA as represented in its DNA.Nucleic Acid Conformation: The spatial arrangement of the atoms of a nucleic acid or polynucleotide that results in its characteristic 3-dimensional shape.Genes, ras: Family of retrovirus-associated DNA sequences (ras) originally isolated from Harvey (H-ras, Ha-ras, rasH) and Kirsten (K-ras, Ki-ras, rasK) murine sarcoma viruses. Ras genes are widely conserved among animal species and sequences corresponding to both H-ras and K-ras genes have been detected in human, avian, murine, and non-vertebrate genomes. The closely related N-ras gene has been detected in human neuroblastoma and sarcoma cell lines. All genes of the family have a similar exon-intron structure and each encodes a p21 protein.Genomic Library: A form of GENE LIBRARY containing the complete DNA sequences present in the genome of a given organism. It contrasts with a cDNA library which contains only sequences utilized in protein coding (lacking introns).Amino Acid Substitution: The naturally occurring or experimentally induced replacement of one or more AMINO ACIDS in a protein with another. If a functionally equivalent amino acid is substituted, the protein may retain wild-type activity. Substitution may also diminish, enhance, or eliminate protein function. Experimentally induced substitution is often used to study enzyme activities and binding site properties.Mycobacterium avium Complex: A complex that includes several strains of M. avium. M. intracellulare is not easily distinguished from M. avium and therefore is included in the complex. These organisms are most frequently found in pulmonary secretions from persons with a tuberculous-like mycobacteriosis. Strains of this complex have also been associated with childhood lymphadenitis and AIDS; M. avium alone causes tuberculosis in a variety of birds and other animals, including pigs.Time Factors: Elements of limited time intervals, contributing to particular results or situations.Seawater: The salinated water of OCEANS AND SEAS that provides habitat for marine organisms.Immunoglobulin Fragments: Partial immunoglobulin molecules resulting from selective cleavage by proteolytic enzymes or generated through PROTEIN ENGINEERING techniques.Bacterial Outer Membrane Proteins: Proteins isolated from the outer membrane of Gram-negative bacteria.Naegleria: A free-living soil amoeba pathogenic to humans and animals. It occurs also in water and sewage. The most commonly found species in man is NAEGLERIA FOWLERI which is the pathogen for primary amebic meningoencephalitis in primates.DNA, Archaeal: Deoxyribonucleic acid that makes up the genetic material of archaea.Borrelia burgdorferi Group: Gram-negative helical bacteria, in the genus BORRELIA, that are the etiologic agents of LYME DISEASE. The group comprises many specific species including Borrelia afzelii, Borellia garinii, and BORRELIA BURGDORFERI proper. These spirochetes are generally transmitted by several species of ixodid ticks.Muridae: A family of the order Rodentia containing 250 genera including the two genera Mus (MICE) and Rattus (RATS), from which the laboratory inbred strains are developed. The fifteen subfamilies are SIGMODONTINAE (New World mice and rats), CRICETINAE, Spalacinae, Myospalacinae, Lophiomyinae, ARVICOLINAE, Platacanthomyinae, Nesomyinae, Otomyinae, Rhizomyinae, GERBILLINAE, Dendromurinae, Cricetomyinae, MURINAE (Old World mice and rats), and Hydromyinae.Reverse Transcriptase Polymerase Chain Reaction: A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.Campylobacter coli: A species of gram-negative, rod-shaped bacteria isolated from the intestinal tract of swine, poultry, and man. It may be pathogenic.Calluna: A plant genus of the family ERICACEAE.Conjunctivitis, Viral: Inflammation, often mild, of the conjunctiva caused by a variety of viral agents. Conjunctival involvement may be part of a systemic infection.HLA-DQ Antigens: A group of the D-related HLA antigens found to differ from the DR antigens in genetic locus and therefore inheritance. These antigens are polymorphic glycoproteins comprising alpha and beta chains and are found on lymphoid and other cells, often associated with certain diseases.Methane: The simplest saturated hydrocarbon. It is a colorless, flammable gas, slightly soluble in water. It is one of the chief constituents of natural gas and is formed in the decomposition of organic matter. (Grant & Hackh's Chemical Dictionary, 5th ed)DNA, Complementary: Single-stranded complementary DNA synthesized from an RNA template by the action of RNA-dependent DNA polymerase. cDNA (i.e., complementary DNA, not circular DNA, not C-DNA) is used in a variety of molecular cloning experiments as well as serving as a specific hybridization probe.NevadaMicrobial Sensitivity Tests: Any tests that demonstrate the relative efficacy of different chemotherapeutic agents against specific microorganisms (i.e., bacteria, fungi, viruses).Oligodeoxyribonucleotides: A group of deoxyribonucleotides (up to 12) in which the phosphate residues of each deoxyribonucleotide act as bridges in forming diester linkages between the deoxyribose moieties.Electrophoresis: An electrochemical process in which macromolecules or colloidal particles with a net electric charge migrate in a solution under the influence of an electric current.Glutathione S-Transferase pi: A glutathione transferase that catalyzes the conjugation of electrophilic substrates to GLUTATHIONE. This enzyme has been shown to provide cellular protection against redox-mediated damage by FREE RADICALS.RNA Probes: RNA, usually prepared by transcription from cloned DNA, which complements a specific mRNA or DNA and is generally used for studies of virus genes, distribution of specific RNA in tissues and cells, integration of viral DNA into genomes, transcription, etc. Whereas DNA PROBES are preferred for use at a more macroscopic level for detection of the presence of DNA/RNA from specific species or subspecies, RNA probes are preferred for genetic studies. Conventional labels for the RNA probe include radioisotope labels 32P and 125I and the chemical label biotin. RNA probes may be further divided by category into plus-sense RNA probes, minus-sense RNA probes, and antisense RNA probes.Receptors, Calcitriol: Proteins, usually found in the cytoplasm, that specifically bind calcitriol, migrate to the nucleus, and regulate transcription of specific segments of DNA with the participation of D receptor interacting proteins (called DRIP). Vitamin D is converted in the liver and kidney to calcitriol and ultimately acts through these receptors.Taiwan

*  Chlamydophila pneumoniae infection of the central nervous system in patients...
RFLP, restriction fragment length polymorphism. Chlamydophila pneumoniae is an obligate ......
http://jnnp.bmj.com/content/75/1/152
*  Irruption of genomics in the search for disease related genes | Gut
Construction of a genetic linkage map in man using restriction fragment length polymorphisms. Am J ... These fragments were directly sequenced. Because the fragments are generated by inducing DNA breaks ... Thus, DNA of BACs was broken down again to fragments of about one thousand base pairs in size. ... It is possible from the knowledge of the sequence of these fragments to reconstruct that of the BAC ......
http://gut.bmj.com/content/52/suppl_2/ii1
*  Molecular analysis of environmental and human isolates of Salmonella typhi.
Staphylococcus aureus outbreak using restriction fragment length polymorphisms of genomic DNA. J ... Altwegg M, Hickman-Brenner FW, Farmer JJ., 3rd Ribosomal RNA gene restriction patterns provide ... after digestion of chromosomal DNA with three restriction endonucleases: XbaI (5'-TCTAGA-3'), AvrII ... among Malaysian isolates of Salmonella typhi as detected by ribosomal RNA gene restriction patterns ......
http://pubmedcentralcanada.ca/pmcc/articles/PMC167795/
*  Esophageal Histoplasmosis - The Gastrointestinalatlas Gastrointestinal -...
Polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) are available. ......
http://gastrointestinalatlas.com/english/esophageal_histoplasmosis.html
*  Browsing Electronic Theses and Dissertations (ETD) by Title
Title: Y-specific restriction fragment length polymorphisms In Southern African populations.  ... ... Browsing Electronic Theses and Dissertations ETD by Title. Browsing Electronic Theses and Dissertations ETD by Title. Login. WIReDSpace Home. Electronic Theses and Dissertations ETD. → Browsing Electronic Theses and Dissertations ETD by Title. Browsing Electronic Theses and Dissertations ETD by Title. Or enter first few letters:. Sort by: title issue date submit date Order: ascending descending Results: 5 10 20 40 60 80 100. Now showing items 9461-9480 of 9485. Previous Page. Title:. X-linked hypophosphatemia in a South African population.  Author:. Title:. X-Men: the adventures of South African Fanboys and other tales of textual poaching.  Author:. Title:. Ximatsatsa: exploring genre in contemporary Tsonga popular music.  Author:. Title:.  Author:. Title:. Yeah Baby, yeah. A case study of a film’s “shagadellic” transition into Italian as packaged on a DVD.  Author:. Title:....
http://wiredspace.wits.ac.za/handle/10539/45/browse?rpp=20&order=ASC&sort_by=1&etal=-1&type=title&starts_with=X
*  restriction endonuclease
restriction fragment length polymorphism - n variation in the length of a restriction fragment ... the restriction site). Also called a restriction enzyme. * * * restriction endonuclease n ... restriction endonuclease - noun any of the enzymes that cut nucleic acid at specific restriction ... RecA-assisted restriction endonuclease cleavage - RecA assisted restriction endonuclease cleavage. ......
http://en.academic.ru/dic.nsf/mwc/113974/restriction
*  cleaved amplified polymorphic sequence
Following PCR amplification of a locus, the amplicon is treated with a restriction endonuclease. If ... Restriction fragment length polymorphism - A Restriction fragment length polymorphism (or RFLP, ... See also: restriction fragment length polymorphism. Glossary of Biotechnology for Food and ... It is an extension to the Restriction Length Fragment Polymorphism (RFLP) method, using polymerase ......
http://biotechnology.enacademic.com/555/cleaved_amplified_polymorphic_sequence
*  DI-fusion A Pvu II RFLP in the human ADH3 gene.
Polymorphism, Genetic Polymorphism, Restriction Fragment Length Note : Journal Article. Langue : * ... An anonymous DNA probe (LAMP 92) detects a Pvu II polymorphism on human chromosome 9 [D9S29] par ......
http://difusion.ulb.ac.be/vufind/Record/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/58554/Details
*  Prodia Penunjang Penelitian
PCR-Restriction Fragment Length Polymorphism. * Real Time PCR (Fluoresence dye dan Probes) ......
http://prodia.co.id/en/ProdukLayanan/PenunjangPenelitian/
*  Forensic Science: Forensic Science Tools And Techniques
RFLP - Restriction Fragment Length Polymorphism molecular genetics techniques; rflp; ... Read ... Topics include digital imaging, legal restrictions related to personal privacy and electronic ......
http://forensicsciencebester.blogspot.com/2014/05/forensic-science-tools-and-techniques.html
*  Validation Studies on the Forensic Analysis of Restriction Fragment Length Polymorphism (RFLP) on LE
Validation Studies on the Forensic Analysis of Restriction Fragment Length Polymorphism RFLP on LE Agarose Gels Without Ethidium Bromide: Effects of Contaminants, Sunlight, and the Electrophoresis of Varying Quantities of Deoxyribonucleic Acid DNA. This document is part of your ASTM Compass subscription. Volume 39, Issue 3 May 1994 Validation Studies on the Forensic Analysis of Restriction Fragment Length Polymorphism RFLP on LE Agarose Gels Without Ethidium Bromide: Effects of Contaminants, Sunlight, and the Electrophoresis of Varying Quantities of Deoxyribonucleic Acid DNA Received 30 November 1992; accepted 0 October 1993 CODEN: JFSOAD   Format Pages Price  . Abstract This study was designed to analyze the effects of sunlight, various contaminants those found typically in forensic samples and the electrophoresis of varying quantities of DNA on the restriction fragment length polymorphism RFLP patterns produced from DNA isolated from blood and semen stains. The results demonstrate that high molecula...
http://astm.org/DIGITAL_LIBRARY/JOURNALS/FORENSIC/PAGES/JFS13649J.htm
*  Restriction fragment length polymorphism
... In molecular biology, 'restriction fragment length polymorphism', or RFLP, is a technique that exploits variations in homologous DNA sequences. In 'RFLP analysis', the DNA sample is broken into pieces and digested by restriction enzymes and the resulting 'restriction fragments' are separated according to their lengths by gel electrophoresis. RFLP analysis was an important tool in genome mapping, localization of genes for genetic disorder s, determination of risk for disease, and paternity testing. Analysis technique. The resulting DNA fragments are then separated by length through a process known as agarose gel electrophoresis, and transferred to a membrane via the Southern blot procedure. Each fragment length is considered an allele, and can be used in genetic analysis. Schematic for RFLP by VNTR length variation. In the first schematic, a small segment of the genome is being detected by a DNA probe thicker line. In allele "c" there are five repeats in the VNTR, and the probe detects a longer fragment b...
https://en.wikipedia.org/wiki/Restriction_fragment_length_polymorphism
*  Project Manager, Microbiology research scientist resume in Jersey City, NJ, 07304 - September 201
... 1. Jersey City, NJ • Tel 646.***.**** • wysrdz@r.postjobfree.com. PhD, Molecular Microbiology 2009 The Tamil Nadu Dr. 2-D Gel Electrophoresis Immunoprecipitation Polymerase Chain Reaction PCR Restriction Fragment Length Polymorphism RFLP Single Strand Conformational Polymorphism SSCP DNA sequencing Immunohistochemistry ELISA Cytogenetics. Indian Council of Medical Research ICMR Fellow at Sankara Nethralaya, Chennai, Tamil Nadu, India 2001 – 2006. SENIOR RESEARCH FELLOW 2004 - 2006 Continued education in microbiology and numerous clinical techniques as a participant in the ICMR-funded project Polymerase chain reaction for the detection of Mycobacterium fortuitum and Mycobacterium chelonae genomes in vitreous, epiretinal membranes and blood of Eales disease patients. RESEARCH FELLOW 2001 – 2004 Awarded a prestigious ICMR fellowship to conduct research for a project titled Detection and molecular characterization of mycobacteria by Polymerase Chain Reaction PCR Restriction Fragment Length Polymorphism RFLP a...
http://postjobfree.com/resume/wysrdz/research-scientist-medical-pp-jersey-city-nj-07304
*  Amplified fragment length polymorphism
... 1 AFLP uses restriction enzymes to digest genomic DNA, followed by ligation of adaptors to the sticky ends of the restriction fragment s. This selection is achieved by using primer s complementary to the adaptor sequence, the restriction site sequence and a few nucleotides inside the restriction site fragments as described in detail below. Although AFLP is commonly referred to as "Amplified fragment length polymorphism", the resulting data are not scored as length polymorphisms, but instead as presence-absence polymorphisms. 2 AFLP-PCR is a highly sensitive method for detecting polymorphism s in DNA. Digestion of total cellular DNA with one or more restriction enzyme s and ligation of restriction half-site specific adaptors to all restriction fragments. Selective amplification of some of these fragments with two PCR primers that have corresponding adaptor and restriction site specific sequences. Image:AFLP phylogeny analysis using a dendrogram The AFLP technology has the capability to detect various poly...
https://en.wikipedia.org/wiki/Amplified_fragment_length_polymorphism
*  What is typing
There are a wide range of phenotypic and genotypic typing methods available for epidemiological studies of bacteria. The problem is exacerbated by the lack of standardised typing methods that can be widely used and provide adequate strain discrimination for good quality epidemiological analyses. Although a range of phenotypic methods have been described for typing campylobacters including biotyping, serotyping and phage typing schemata , these are insufficiently discriminatory and, in the case of phage- and serotyping schemes, not widely available. These include plasmid profiling, ribotyping, and various polymerase chain reaction PCR -based techniques. Most of these methods provide a higher level of discrimination than the phenotypic techniques, but without exception they suffer from a lack of standardisation. Flagellin gene restriction fragment length polymorphism analysis fla -PCR. This method involves amplification of the flagellin gene by PCR and subsequent digestion of the PCR product with a restriction ...
http://campynet.vetinst.dk/typing.htm
*  Detection of Microorganisms in Granulomas That Have Been Formalin-Fixed: Review of the Literature Re
Lung cavity in a patient with hyperlipidemia and gout Caseous necrosis, sarcoidal granuloma, positive AFB Formalin-fixed, paraffin-embedded hsp65 gene followed by sequencing M. Old cavitary tuberculosis 5 patients and COPD 1 Caseous necrosis, bronchiectasis 2 of 6 with positive AFB Formalin-fixed, paraffin-embedded hsp65 gene followed by sequencing M. Masses in 2 HIV positive patients Spindle cell pseudotumor with abundant AFB staining Formalin-fixed, paraffin-embedded hsp65 gene followed by sequencing or restriction fragment polymorphisms M. Lymphadenitis in children Caseous granulomas 1 of 2 with positive AFB Tissue 16S rRNA gene and the IS 1245 specific sequence for M. Lymphadenitis in children Necrotizing granulomas 3 of 22 positive with AFB Culture Probe not specified M. 5 patients with chronic skin lesions in extremities, recurrent, some thought to be sarcoid or swimming pool granulomas Granulomas, 2 of them with positive AFB Formalin-fixed, paraffin-embedded 16S rRNA gene then sequencing M. Multiple, p...
http://hindawi.com/journals/scientifica/2012/494571/tab2/
*  Restriction digest
This enzymatic technique can be used for cleaving DNA molecules at specific sites, ensuring that all DNA fragments that contain a particular sequence have the same size; furthermore, each fragment that contains the desired sequence has the sequence located at exactly the same position within the fragment. These enzymes are called restriction endonucleases or restriction enzymes, and they are able to cleave DNA molecules at the positions at which particular short sequences of bases are present. A given restriction enzyme cuts DNA segments within a specific nucleotide sequence, at what is called a restriction site. This allows the insertion of almost any specific fragment of DNA into plasmid vectors, which can be efficiently "cloned" by insertion into replicating bacterial cells. After restriction digest, DNA can then be analysed using agarose gel electrophoresis. The molecules travel at different rates and therefore end up at different distances depending on their net charge more highly charged particles trave...
https://en.wikipedia.org/wiki/Restriction_digest
*  DNA fingerprinting | Britannica.com
DNA fingerprinting. Britannica.com. DNA fingerprinting. Article. Web sites. Written by: The Editors of Encyclop dia Britannica. View All Media 4. Alternative titles: DNA profiling; DNA typing; genetic fingerprinting; genotyping; identity testing DNA fingerprinting , also called DNA typing, DNA profiling, genetic fingerprinting, genotyping, or identity testing ,. DNA fingerprinting: restriction enzyme technique Encyclopædia Britannica, Inc. In Jeffreys’s original approach, which was based on restriction fragment length polymorphism RFLP technology, the DNA was then cut at specific points along the strand with proteins known as. Some of the concerns with DNA fingerprinting, and specifically the use of RFLP, subsided with the development of PCR- and STR-based approaches. Email. science. What made you want to look up DNA fingerprinting. Britannica Stories Behind The News / Society Death at Umpqua. 2015 Encyclop dia Britannica, Inc. MLA style: "DNA fingerprinting". Encyclop dia Britannica. Encyclop dia Britannica ...
http://britannica.com/science/DNA-fingerprinting
*  Genotyping of Kappa-Casein Locus by PCR-RFLP in Brown Swiss Cattle Breed
... Journals by Subject Agricultural Sciences Animal Sciences Applied Sciences Business Sciences Earth Sciences Engineering Information Technology Medical Sciences Molecular Sciences Pharmacology Social Sciences. Genotyping of Kappa-Casein Locus by PCR-RFLP in Brown Swiss Cattle Breed. Abstract: A Polymerase Chain Reaction-Restriction Fragment Length Polymorphism PCR-RFLP test was performed on DNA samples extracted from blood samples of Brown Swiss cattle to detect genotype frequency of the bovine kappa-casein CNS3 locus. A 351 bp fragment of CSN3 was amplified and digested with Hinf I restriction enzymes. Three genotypes were observed, frequencies were 19.35, 20.43 and 60.22% for AA, BB and AB, respectively. Two genetic variants CSN3 A and CNS3 B were identified and the allelic frequencies were estimated as 0.495 and 0.505, respectively. Milk protein genetic polymorphism has received considerable research interest in recent years because of possible associations between milk protein genotypes and economical...
http://medwelljournals.com/fulltext/?doi=javaa.2009.779.781
*  Browsing IJA Volume 58, Issue 1, 2006 by Author "Erdogan, Orhan"
browsing ija volume issue by author erdogan orhan my evols home search browse by author by subject by title by date communities collections contact scholarspace uh system uhm library hamilton library homepage research tools personal services about the library evols home israeli journal of aquaculture bamidgeh volume issues ija volume issue browsing ija volume issue by author javascript is disabled for your browser some features of this site may not work without it browsing ija volume issue by author erdogan orhan sort by title issue date submit date order ascending descending results now showing items of authentication of fish species using a simple pcr rflp method hisar oicay erdogan orhan aksakal ecrument hisar sükriye aras israeli journal of aquaculture bamigdeh a polymerase chain reaction restriction fragment length polymorphism pcr rflp method was developed as a tool to prevent commercial frauds in fish products the pcr was used to ampli fy the cytochrome b gene part of the now showing items of search se...
http://evols.library.manoa.hawaii.edu/handle/10524/19001/browse?value=Erdogan, Orhan&type=author
*  Genotype
The 'Genotype' is that part DNA sequence of the genetic makeup of a cell, and therefore of an organism or individual, which determines a specific characteristic phenotype of that cell/organism/individual. Genotype is one of three factors that determine phenotype, the other two being inherited epigenetic factors, and non-inherited environmental factors. DNA mutations which are acquired rather than inherited, such as cancer mutations, are not part of the individual's genotype; hence, scientists and physicians sometimes talk for example about the geno type of a particular cancer, that is the genotype of the disease as distinct from the diseased. Genotypic and genomic sequence. B - dominant genotype and b - recessive genotype Genotype and phenotype. There are three available genotypes, PP homozygous dominant, Pp heterozygous, and pp homozygous recessive. A SNP occurs when corresponding sequences of DNA from different individuals differ at one DNA base, for example where the sequence AAGCCTA changes to AAGCTTA. SN...
https://en.wikipedia.org/wiki/Genotype
*  J A Fuhrman
... add/edit. You are here: Scientific Experts USA. University of Southern California Fuhrman J A Fuhrman. Research Topics plankton seawater bacteria marine biology bacterial physiology biodiversity archaea ecosystem collodion photobiology virology poliovirus enterovirus radiography greenhouse effect rhodopsin algae oceans and seas glass cellulose ecology filtration fresh water ribosomal dna restriction fragment length polymorphism genomics geography viral rna energy metabolism water microbiology. J A Fuhrman Summary Affiliation: University of Southern California Country: USA. Publications A comparison of taxon co-occurrence patterns for macro- and microorganisms M Claire Horner-Devine School of Aquatic and Fishery Sciences, University of Washington, Seattle, Washington 98195, USA Ecology 88:1345-53. 2007 Metagenomics and its connection to microbial community organization Jed A Fuhrman Department of Biological Sciences, University of Southern California Los Angeles CA 90089 USA F1000 Biol Rep 4:15. 2012 Exte...
http://labome.org/expert/usa/university/fuhrman/j-a-fuhrman-493279.html
*  NA07051
Login. View Cart. Advanced Clinical Data Search. Search by Catalog ID. Species. Search Help. BIOREPOSITORIES. NIGMS. Stem Cells. HapMap. CEPH Resources. All Biorepositories. Services. Cell Cultures. Stem Cells. All Services. Ordering. Online Ordering. Assurance Form Signatory. Fibroblast Cultures. Lymphoblast Cultures. DNA Samples. Culture Medium. Adiposal Stromal Cultures. Fetal Bovine Serum. Melanocyte Cultures. Search Help. Contact Us. DNA from LCL. CEPH/UTAH PEDIGREE 13292 INTERNATIONAL HAPMAP PROJECT - CEPH PLATE II INTERNATIONAL HAPMAP PROJECT - CEPH NA CUSTOM SERVICE PLATE 02. No Data. No Data. Phenotypic Data. Repository NIGMS Human Genetic Cell Repository. Subcollection CEPH Repository Linkage Families PIGI Consented Sample. Quantitation Method Please see our FAQ. Cell Type B-Lymphocyte. Family Member 12. Relation to Proband maternal grandfather. Remarks Maternal Grandfather Same as 13294-09. IDENTIFICATION OF SPECIES OF ORIGIN Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosph...
https://catalog.coriell.org/0/Sections/Search/Sample_Detail.aspx?Ref=GM07051
*  DNA and Technology
... News A-Z. Health A-Z. Medical News 'Tweets'. Health. News. DNA and Technology. DNA technology in forensics DNA is unique. Because it is unique, the ability to examine DNA found at a crime scene is a very useful forensic tool. The common methods used to identify and describe the DNA profile includes - Restriction fragment length polymorphism RFLP and Short tandem repeat profiling STR. Restriction fragment length polymorphism RFLP In RFLP, the DNA is cut into segments of varying lengths by an enzyme, then the segments are separated out on the basis of size using a technique called electrophoresis. Short tandem repeat profiling STR Short tandem repeat profiling STR involves use of an enzyme to make many copies of a small section of the DNA. This section is then cut into pieces by another enzyme, and separated by electrophoresis. The two exciting techniques that have come up include the genome sequencing technology and the DNA chip technology. It is estimated that the human genome has about 30,000 genes, whi...
http://news-medical.net/health/DNA-and-Technology.aspx
*  Oligomer restriction
... A labeled oligonucleotide probe is hybridized to a target DNA, and then treated with a restriction enzyme. If, however, the target DNA does not exactly match the probe, the restriction enzyme will have no effect on the length of the probe. The OR technique, now rarely performed, was closely associated with the development of the popular polymerase chain reaction PCR method. Example History Problems Relationship to PCR References. Part of the probe includes the Recognition site for the restriction enzyme Dde I underlined. In part 2, the same probe is shown hybridized to a target DNA which includes a single base mutation here the mutation responsible for Sickle Cell Anemia, or SCA. The Oligomer Restriction technique was developed as a variation of the Restriction Fragment Length Polymorphism RFLP assay method, with the hope of avoiding the laborious Southern blot ting step used in RFLP analysis. Saiki RK, Bugawan TL, Mullis KB, and Erlich HE "Analysis of enzymatically amplified beta-globin and HLA-DQa DNA ...
https://en.wikipedia.org/wiki/Oligomer_restriction
*  Cleaved amplified polymorphic sequence
... the cleaved amplified polymorphic sequence or caps method is a technique in molecular biology for the analysis of genetic marker s it is an extension to the restriction fragment length polymorphism rflp method using polymerase chain reaction pcr to more quickly analyse the results like rflp caps works on the principle that genetic differences between individuals can create or abolish restriction endonuclease restriction sites and that these differences can be detected in the resulting dna fragment length after digestion in the caps method pcr amplification is directed across the altered restriction site and the products digested with the restriction enzyme when fractionated by agarose or acrylamide gel electrophoresis the digested pcr products will give readily distinguishable patterns of bands alternatively the amplified segment can be analyzed by allele specific oligonucleotide aso probes a process that can often be done by a simple dot blot see also rflp references external links http www ncbi nlm nih...
https://en.wikipedia.org/wiki/Cleaved_amplified_polymorphic_sequence
*  Index of molecular biology articles
C terminus - cancer - candidate gene - Canonical sequence - cap - cap site - carboxyl terminus - carcinoma - carrier - CAT assay - CCAAT box - cDNA - cDNA clone - cDNA library - cell - centimorgan - centromere - chain terminator - chaperone protein - chromosome - Chromosomal translocation - chromosome walking - CIS - cistron - clone genetics - clone noun - clone verb - cloning - coding sequence - coding strand - codon - codon usage bias - competent - complementary - conformational epitope - congenital - consensus sequence - conservative substitution - conserved - contig - cosmid - craniosynostosis - cystic fibrosis - cytogenetic map - cytosine - CpG. gel electrophoresis - gel shift - gel shift assay - gene - gene amplification - gene conversion - gene expression - gene mapping - gene pool - gene therapy - gene transfer - genetic code - genetic counseling - genetic map - genetic marker - genetic screening - genetically modified mouse - genome - genomic blot - genomic clone - genomic library - genotype - germ l...
https://en.wikipedia.org/wiki/Index_of_molecular_biology_articles
*  DRAT - Directed terminal Restriction Analysis Tool | The James Hutton Institute
The James Hutton Institute. It is applicable to any organisms/gene targets and can be directed to search for enzymes, or combinations of enzymes, to identify diagnostic Terminal Restriction Fragments TRFs. The program uses regular expression matching to discover cut sites in each sequence for each the enzymes in the enzyme file unless a single enzyme is specified It then generates an mxn table of 5' and 3' terminal fragments lengths where m is the number of sequences and n the number of enzymes and writes this table to a delimited text file. DRAT.pl a 6k PL file Drat a 970KB application file Drat an 18KB CPP file Bionetc.512 a 32KB file Lib a new sub-folder TestA.fasta a small fasta file used for training and troubleshooting purposes.It is worth storing a copy of this fasta file as it can be used for troubleshooting purposes see Troubleshooting. The aim of DRAT is to determine terminal restriction fragments TRFs that are common within a taxonomic group and that separates these taxonomic groups. Using ...
http://hutton.ac.uk/drat
*  Videos for dna fingerprinting - Homework Help Videos - Brightstorm
... Toggle navigation. Browse Subjects. Math. Pre-Algebra. Algebra. Geometry. Algebra 2. Trigonometry. Precalculus. Calculus. Science. Biology. Chemistry. Physics. English. Grammar. Writing. Literature. Test Prep. SAT. ACT. ACT Red Book. PSAT. AP US Gov. AP US History. AP Biology. AP Calculus AB. College. Get Better Grades. College Application. College Essay. Financial Aid. Search. or Find by textbook. Study. Math. Science. English. Test Prep. Sign in. Teacher membership. School membership. Start Your Free Trial. 3 Videos for "dna fingerprinting" RFLP - DNA Fingerprinting Science › Biology › Molecular Biology The RFLP method of DNA Fingerprinting. Tags: RFLP DNA fingerprinting restriction fragment length polymorphism. PCR - DNA Fingerprinting Science › Biology › Molecular Biology The PCR method of DNA Fingerprinting. Tags: PCR DNA fingerprinting gel electrophoresis polymerase chain reaction. Biotech: DNA Fingerprinting Test Prep › AP Biology › AP Biology Videos. Tags: Gel electrophoresis RFLP PCR. 1 About Ho...
http://brightstorm.com/tag/dna-fingerprinting/
*  Template:Molecular Genetics Methods
template molecular genetics methods template molecular genetics methods navbox name molecular genetics methods title molecular genetics key methods of study listclass hlist state group experimental list gel electrophoresis molecular cloning northern blot promoter bashing restriction digest site directed mutagenesis southern blot group bioinformatics list gene sequencing microarray restriction fragment length polymorphism str analysis...
https://en.wikipedia.org/wiki/Template:Molecular_Genetics_Methods
*  JCVI: Effects of Human TRIM5alpha Polymorphisms on Antiretroviral Function and Susceptibility to Hum
... an Immunodeficiency Virus Infection. Sustainable Lab. Publications. Education. Press. Careers. Contact. Listing. Publications. Citation. Javanbakht, H., An, P., Gold, B., Petersen, D. W., O'Brien, S. J., Kirk, G. D., Detels, R., Buchbinder, S., Donfield, S., Shulenin, S., Song, B., Perron, M. J., Stremlau, M., Sodroski, J., Dean, M., Winkler, C. Effects of Human TRIM5alpha Polymorphisms on Antiretroviral Function and Susceptibility to Human Immunodeficiency Virus Infection. Virology. 2006 Oct 10; 354 1 : 15-27. PubMed Citation. Abstract. TRIM5alpha acts on several retroviruses, including human immunodeficiency virus HIV-1, to restrict cross-species transmission. Using natural history cohorts and tissue culture systems, we examined the effect of polymorphism in human TRIM5alpha on HIV-1 infection. In African Americans, the frequencies of two non-coding SNP variant alleles in exon 1 and intron 1 of TRIM5 were elevated in HIV-1-infected persons compared with uninfected subjects. By contrast, the frequency of...
http://jcvi.org/cms/publications/listing/abstract/article/effects-of-human-trim5alpha-polymorphisms-on-antiretroviral-function-and-susceptibility-to-human-imm/
*  Blood Journal | Prevalence of the Inactivating 609C→T Polymorphism in the NAD(P)H:Quinone Oxidored
Prevalence of the Inactivating 609C→T Polymorphism in the NAD P H:Quinone Oxidoreductase NQO1 Gene in Patients With Primary and Therapy-Related Myeloid Leukemia. Prevalence of the Inactivating 609 C→T Polymorphism in the NAD P H:Quinone Oxidoreductase NQO1 Gene in Patients With Primary and Therapy-Related Myeloid Leukemia. Smith. Of the 45 leukemia patients who had clonal abnormalities of chromosomes 5 and/or 7, 7 16% were homozygous for the inactivating polymorphism, 17 38% were heterozygous, and 21 47% had 2 wild-type alleles for NQO1. Thus, the frequency of an inactivating polymorphism in NQO1 appears to be increased in this cohort of myeloid leukemias, especially among those with t-AML or an abnormality of chromosomes 5 and/or 7. The frequency of the NQO1 polymorphism was significantly increased among all 104 patients P = .050 and among the 56 patients with t-AML P = .036 compared with the frequency expected. Frequency of the NQO1 Polymorphism in Primary and Therapy-Related Myeloid Leukemia. Frequency of ...
http://bloodjournal.org/content/94/2/803.full?sso-checked=true
*  JCVI: Lack of association between TLR4 Asp299Gly polymorphism and atherosclerosis: evidence from met
... a-analysis. Home. About. Research. Sustainable Lab. Publications. Education. Giving. Press. Careers. Contact. Listing. About. . Publications. Citation. Zhang K, Zhang L, Zhou B, Wang Y, Song Y, Rao L. Lack of association between TLR4 Asp299Gly polymorphism and atherosclerosis: evidence from meta-analysis. Thrombosis research. 2012 Aug 01;. External Citation. Abstract. INTRODUCTION: Toll like receptor 4 TLR4 expression was found to increase markedly in human atherosclerotic lesions, notably on macrophages and endothelial cells. TLR4 Asp299Gly polymorphism was associated with a blunted receptor activity and a subsequently diminished inflammatory response, and may subsequently reduce atherosclerosis AS risk. However, the results of molecular epidemiological studies remained inconsistent. MATERIALS AND METHODS: The PubMed, CNKI databases were searched for all articles available. The OR corresponding to the 95% confidence interval 95% CI was used to assess the association between TLR4 Asp299Gly polymorphism an...
http://jcvi.org/cms/publications/listing/abstract/article/lack-of-association-between-tlr4-asp299gly-polymorphism-and-atherosclerosis-evidence-from-meta-anal/
*  Java polymorphism question - Stack Overflow
... Stack Overflow. Meta Stack Overflow. Stack Overflow Careers. stack overflow careers. Stack Overflow Questions. Java polymorphism question. abstract class A { abstract void a1 ; void a2 { } } class B extends A { void a1 { } void a2 { } } class C extends B { void c1 { } } and: A x = new B ; C y = new C ; A z = new C ; What are four valid examples of polymorphic method calls. java polymorphism scjp share. improve this question. add a comment. Answer C won't compile method isn't defined in the declared class. improve this answer. answered Dec 24 '09 at 23:58. add a comment. up vote 8 down vote. Polymorphism is simply defined as when the reference type and object type are different. The reference type of anim Animal is different than its object type Dog so anim is a polymorphic reference. Dynamic binding means that the method that actually runs is the method that is farthest down the class hierarchy between the reference type and object type. Being able to have the method that runs depend on the object type i...
http://stackoverflow.com/questions/1960091/java-polymorphism-question
*  TRAF1 Gene Polymorphism Correlates with the Titre of Gp210 Antibody in Patients with Primary Biliary
traf gene polymorphism correlates with the titre of gp antibody in patients with primary biliary cirrhosis table table autoantibody titers in patients subgrouped according to rs traf polymorphism autoantibodies genotype cc genotype tt ama gp sp actin centromere chromatin rfigg ccp scl jo rna poliii ro...
http://hindawi.com/journals/jir/2012/487521/tab5/
*  Gene polymorphism
... thumb right genes which control hair colour are polymorphic a gene is said to be polymorphic if more than one allele occupies that gene s locus within a population http www biology online org dictionary genetic polymorphism a polymorphic variant of a gene may lead to the abnormal expression or to the production of an abnormal form of the gene this may cause or be associated with disease for example a polymorphic variant of the enzyme cyp a in which thymidine replaces cytosine at the gene s nucleotide position encodes a cyp a protein that substitutes phenylalanine with serine at the protein s amino acid position this variant protein has reduced enzyme activity in metabolizing arachidonic acid to the blood pressure regulating eicosanoid hydroxyeicosatetraenoic acid humans bearing this variant in one or both of their cyp a genes have an increased incidence of hypertension ischemic stroke and coronary artery disease cardiol rev jan feb doi crd b e review examples of polymorphic genes drd ankk comt maoa cyp a...
https://en.wikipedia.org/wiki/Gene_polymorphism
*  Rs28363170
rs rs in genetics rs dat vntr is a genetic variation at slc a the gene that encodes the dopamine transporter it is polymorphism as a base pairs vntr in the untranslated region it is an deletion insertion polymorphism dip http www ncbi nlm nih gov snp snp ref cgi rs rs the repeat and the repeat are the most common alleles references further category genetic polymorphisms...
https://en.wikipedia.org/wiki/Rs28363170
*  WHO | WHO Working Group on Polymerase Chain Reaction (PCR) Protocols for Detecting Subtype Influenza
WHO. WHO Working Group on Polymerase Chain Reaction PCR Protocols for Detecting Subtype Influenza A Viruses. Skip to main content. Access Home Alt+0. Navigation Alt+1. Content Alt+2. Search Search the WHO .int site. Submit. Advanced search. Navigation Home. Health topics. Data. Media centre. Publications. Countries. Programmes. Governance. About WHO. Language عربي. 中文. English. Français. Русский. Español. RSS Feed. Youtube. Twitter. Facebook. Google +. iTunes. Play Store. Influenza. Menu. Influenza Surveillance and monitoring Updates FluID. GISRS and laboratory FluNet National Influenza Centres WHO Collaborating Centres for influenza and Essential Regulatory Laboratories WHO H5 Reference Laboratories Antiviral susceptibility surveillance WHO External Quality Assessment Project Shipping and logistic activities. PIP Framework Advisory Group Virus Sharing Benefit Sharing. Vaccines Vaccine viruses Vaccine use. Patient care Clinical management Antivirals. Human animal interface Avian influenza in humans Swine infl...
http://who.int/influenza/gisrs_laboratory/pcr_working_group/en/
*  Adaptation of the Ultrasensitive HIV-1 p24 Antigen Assay to... : JAIDS Journal of Acquired Immune
Text sizing: A. A real-time polymerase chain reaction assay for DBS DNA and/or plasma RNA identified HIV-1 infection in 38 Tanzanian children. The detection rates for the different assays were as follows: DBS-p24, 32 84% of 38 samples; DBS DNA, 30 79% of 38 samples; plasma-p24, 23 85% of 27 samples; and plasma RNA, 30 100% of 30 samples. 11-13 Specifically, in HIV-1 subtype B and C settings, p24-based diagnosis of pediatric HIV-1 infection was found to be similarly sensitive and specific as polymerase chain reaction PCR -based measurement of HIV-1 DNA or RNA. Back to Top. Back to Top. Article Outline Real-time Polymerase Chain Reaction for HIV-1 RNA RNA was extracted using the reagents of the Amplicor HIV-1 Monitor assay, version 1.5 Roche Molecular Diagnostics, Rotkreuz, Switzerland. A total of 38 subjects were identified as HIV-1 infected using the DBS HIV-1 DNA PCR assay n = 72 and/or RT-PCR assay for HIV-1 RNA in plasma n = 30. Testing of frozen plasma samples, which were not available from all 38 infecte...
http://journals.lww.com/jaids/Fulltext/2007/03010/Adaptation_of_the_Ultrasensitive_HIV_1_p24_Antigen.1.aspx
*  7900HT Fast Real-Time PCR System Support | Thermo Fisher Scientific
7900HT Fast Real-Time PCR System Support. Order Support. PCR. Real-Time PCR. Thermo Scientific. Thermo Scientific. Product Selection Guides. Services. Diagnostic Products & Services. Thermo Scientific. Diagnostic Instruments. Browse through the “Guides and Tools” section to access comprehensive product-related support resources. What volumes can be used in my plate on the 7900HT Fast Real-Time PCR System. How do I add a melt curve to my experiment on the 7900HT Fast Real-Time PCR System. How can I convert a file from AQ to RQ, or from RQ to AQ, with the 7900HT Fast Real-Time PCR System. Guide: 7900HT Fast Real-Time PCR System Maintenance and Troubleshooting Guide - Includes Calibration Guide: Relative Quantitation on 7900HT Fast Real-Time PCR System. Guide: User Guide: TaqMan® Array Micro Fluidic Cards Selection Table: Real-Time PCR Master Mixes and Instrument Compatibility. Supplemental Protocol: Rapid Cycling of TaqMan® Array Cards on 7900HT Fast Real-Time PCR System. Overview: Plastic Consumables Thermo Sc...
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*  Thermal Cyclers :: Alpha Laboratories
... Microplate Sealing Films. + Pipette Tips. Pipette Tips - An Overview. + Alpha Pipette Tips. Alpha Pipette Tips - An Overview. + Fastrak. Fastrak Technical Information. + Filter Pipette Tips. Filter Pipette Tips - An Overview. Non-Filter Pipette Tips. + Pipettes. Proline Pipettes. Proline Plus Pipettes. alpha+ Pipettes. + Molecular Biology Products. + Clarit-E Electrophoresis Systems. Clarit-E Electrophoresis Systems. + Horizontal Electrophoresis Systems. Midi Horizontal Electrophoresis. Midi96 Horizontal Electrophoresis System. DNA-VIEW Electrophoresis System. Maxi Horizontal Electrophoresis System. Screen Horizontal Electrophoresis System. Horizontal System Accessories. + Vertical Electrophoresis System. Vertical Electrophoresis. Mini Vertical System. Mini Wide Vertical System. Maxi Vertical System. Maxi Plus Vertical System. Maxi Z Vertical System. Vertical Modular Systems. Vertical Accessories. Electrophoresis Accessories. Molecular Biology Equipment. Thermal Cyclers by Bioer the trusted name in therm...
http://alphalabs.co.uk/product-information/molecular-biology-products/thermal-cyclers/thermal-cyclers.aspx
*  ViiA™ 7 Real-Time PCR System | Thermo Fisher Scientific
ViiA™ 7 Real-Time PCR System. Order Support. PCR. Real-Time PCR. Thermo Scientific. Thermo Scientific. Services. CaptureSelect™ Products & Services. Diagnostic Products & Services. Thermo Scientific. Diagnostic Instruments. High-performance features for maximum productivity Ideal for performing medium- to high-throughput real-time PCR, the ViiA™ 7 System enhances your lab’s productivity. Fully compatible with TaqMan Array Microfluidic Cards TaqMan Assays for real-time PCR analysis provide maximum sensitivity and specificity, and broad dynamic range. Application notes Analysis of microRNA and protein expression on a single platform using the ViiA™ 7 Real-Time PCR System Read how TaqMan Assays and the ViiA™ 7 Real-Time PCR System combine to help identify trends in miRNA and protein expression profiles on a single analytical platform. Videos Tour the instrument Take a tour through the ViiA™ 7 system and learn how features like OptiFlex™, ReadiApp™, and integrated TaqMan content can transform your lab's productiv...
http://thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/real-time-pcr-instruments/viia-7-real-time-pcr-system.html
*  LabWrench | PCR / Thermal Cyclers | Questions | Discussions | Information
PCR / Thermal Cyclers. PCR / Thermal Cyclers. Add Life Technologies Add QuantStudio® 5 Real-Time PCR System to My Bench. Life Technologies QuantStudio 3® Real-Time PCR System Now you can get up and running quickly with the Applied Biosystems® QuantStudio 3 Real-Time PCR System, a simple and affordable real-time PCR solution Add Life Technologies Add QuantStudio 3® Real-Time PCR System to My Bench. Add EPPENDORF Add Mastercycler pro to My Bench. G-Storm GS1 G-STORM GS1 Thermal Cycler Add G-Storm Add GS1 to My Bench. G-Storm GS2 The G-Storm GS2 is the perfect thermal cycler solution for laboratories that require medium to high throughput performance or for labs that have Add G-Storm Add GS2 to My Bench. Compare Equipment In PCR / Thermal Cyclers. Ltd Dynex Technologies Eagle Manufacturing Company Eberbach EDC Biosystems Edinburgh Instruments Edwards EKF Diagnostics Eksigent Elanco Eldex Electron Microscopy Sciences Electrothermal elementar ELGA Ellutia Elma Ultrasonic Cleaners EMCO High Voltage Power Corpor...
http://labwrench.com/?equipment.list/categoryNo/835/PCR---Thermal-Cyclers
*  Thermal cycler
thumb|right|A thermal cycler The 'thermal cycler' also known as a 'thermocycler', 'PCR machine' or 'DNA amplifier' is a laboratory apparatus most commonly used to amplify segments of DNA via the polymerase chain reaction PCR. 1 Thermal cyclers may also be used in laboratories to facilitate other temperature-sensitive reactions, including restriction enzyme digestion or rapid diagnostics. 2 The device has a 'thermal block' with holes where tubes holding the reaction mixtures can be inserted. History Modern innovations Pricing References External links. Rather than cycling through different temperatures, it uses three different water baths at constant temperature between which samples are moved with a robotic arm. The earliest thermal cyclers were designed for use with the Klenow fragment of DNA polymerase I. Since this enzyme is destroyed during each heating step of the amplification process, new enzyme had to be added every cycle. This led to a cumbersome machine based on an automated pipettor, with open reac...
https://en.wikipedia.org/wiki/Thermal_cycler
*  Inverse polymerase chain reaction
... thumb|right|250px|Summary of the inverse PCR process. 'Inverse polymerase chain reaction' 'Inverse PCR' is a variant of the polymerase chain reaction that is used to amplify DNA with only one known sequence. One limitation of conventional PCR is that it requires primers complementary to both termini of the target DNA, but this method allows PCR to be carried out even if only one sequence is available from which primers may be designed. Inverse PCR is especially useful for the determination of insert locations. For example, various retrovirus es and transposon s randomly integrate into genomic DNA. To identify the sites where they have entered, the known, "internal" viral or transposon sequences can be used to design primers that will amplify a small portion of the flanking, "external" genomic DNA. The amplified product can then be sequenced and compared with DNA databases to locate the sequence which has been disrupted. The inverse PCR method involves a series of restriction digest s and ligation, result...
https://en.wikipedia.org/wiki/Inverse_polymerase_chain_reaction
*  TaqMan® Gene Expression Using the QuantStudio™ 12K Flex Real-Time PCR System with OpenArray® Blo
... ck. Order Support. Real-Time PCR. Thermo Scientific. Thermo Scientific. New Products. Diagnostic Products & Services. Thermo Scientific. TaqMan Gene Expression Using the QuantStudio 12K Flex Real-Time PCR System with OpenArray Block. TaqMan® Gene Expression Using the QuantStudio™ 12K Flex Real-Time PCR System with OpenArray® Block. TaqMan® Gene Expression Using the QuantStudio™ 12K Flex Real-Time PCR System with OpenArray® Block. TaqMan OpenArray gene expression workflow on QuantStudio™ 12K Flex System. QuantStudio™ TaqMan OpenArray Real-Time PCR Plates are delivered with assays dried down in the through-holes you specify. Prepare your sample mix: Mix cDNA or the product of your pre-amplification step with TaqMan® OpenArray® Genotyping Master Mix in a TaqMan® OpenArray® 384-well sample plate. TaqMan® OpenArray® Real-Time Master Mix 1.5 mL, 2X TaqMan® OpenArray® Real-Time Master Mix 5 mL, 2X OpenArray® 384-Well Sample Plates OpenArray® 384-Well Barcoded Sample Plates. Load your sample mixes onto the TaqMan...
http://thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/real-time-pcr-applications/gene-expression-using-real-time-pcr/taqman-gene-expression-openarray.html
*  Lysis Buffer | Bio-Rad
In most purification protocols for nucleic acids, proteins, membranes, or organelles, the first step is usually cell lysis. The type of lysis buffer used depends on the types and source of cells, the desired final molecule or structure, and the level of their functionality. The protocols and lysis buffers for proteins are generally different from the lysis buffers used for nucleic acids. There are a number of different types of lysis buffer for protein extraction. The type of lysis buffer used depends on the cell source tissue culture, plant, bacteria, fungi, etc. , and whether the cells are in a structure and the type of structure. For instance, lysis of cells in tissue culture is much easier than lysis of cells in a tissue with a high level of contractile proteins such as skeletal muscle. Most lysis buffers for extraction of proteins, membranes, and organelles contain one or more detergents. Lysis Buffer for Nucleic Acids. Lysing cells for extraction of nucleic acids is in some ways much easier than extract...
http://bio-rad.com/featured/en/lysis-buffer.html
*  A consensus on fungal polymerase chain reaction diagnosis?: A United Kingdom-Ireland evaluation of
a consensus on fungal polymerase chain reaction diagnosis a united kingdom ireland evaluation of polymerase chain reaction methods for detection of systemic fungal infections westminsterresearch home about browse browse by year browse by research community browse by type browse by people browse by full text advanced search latest additions login repository administrators only statistics a consensus on fungal polymerase chain reaction diagnosis a united kingdom ireland evaluation of polymerase chain reaction methods for detection of systemic fungal infections white p lewis and barton richard and guiver malcolm and linton christopher j and wilson steve and smith melvyn and gomez beatriz l and carr michael j and kimmitt patrick t and seaton shila and rajakumar kumar and holyoake tessa and kibbler chris c and johnson elizabeth and hobson richard p and jones brian and barnes rosemary a a consensus on fungal polymerase chain reaction diagnosis a united kingdom ireland evaluation of polymerase chain reaction methods...
http://westminsterresearch.wmin.ac.uk/3185/
*  Non Food Items
... Organic Kingdom. Contact Us. Shopping Cart. Checkout. Non Food Items. Non Food Items. Items 1 to 20 of 141 total Show. Yogurt Making Thermometer Product Size: 1 each $8.50. Add to Cart Yogurt Making Thermometer Learn More. Extra Batch Jar w/lid Product Size: 1 each $16.99. Add to Cart Extra Batch Jar w/lid Learn More. Personal Blender - PB150 Product Size: 1 each $103.75. Add to Cart Personal Blender - PB150 Learn More. Sproutmaster Triple Product Size: 1 set $52.99. Add to Cart Sproutmaster Triple Learn More. Sproutmaster Single Product Size: 1 set $20.75. Add to Cart Sproutmaster Single Learn More. Crew Socks, Organic, Natural 8-10 Product Size: 1 pr. Add to Cart Crew Socks, Organic, Natural 8-10 Learn More. Crew Socks, Organic, Natural 10-13 Product Size: 1 pr. Add to Cart Crew Socks, Organic, Natural 10-13 Learn More. Learn More. Natural Paper Towels Product Size: Case/30 $104.50. Add to Cart Natural Paper Towels Learn More. Natural Paper Towels Product Size: 1 Roll $3.75. Add to Cart Natural Paper T...
http://organickingdom.com/non-food-items.html?dir=desc&limit=20&mode=list&order=position
*  User:Bill Flanagan - OpenWetWare
... My name is Bill Flanagan. 5.2 Annotation 5.2.1 Microsoft: Shared Annotation Notification Paper 5.2.2 Online Protocol Annotation 5.2.3 One Big Lab 5.2.4 Neo Note 5.2.5 PDF Annotation 5.2.6 Wired 5.2.7 Information seeking behavior of academic scientists 5.2.8 Biomedical Searching. The tools are available as no-cost downloads to academic researchers and scientists at http://research.microsoft.com/en-us/collaboration/tools. Project Trident was developed by Microsoft Research's External Research Division specifically to support the scientific community. Its goals are to enhance the user experience on computing devices, reduce the cost of writing and maintaining software, and invent novel computing technologies. showpdf http://research.microsoft.com/pubs/64568/tr-2002-74.pdf /showpdf. showpdf http://research.microsoft.com/pubs/69880/tr-2001-87.pdf /showpdf. Posted Dec 2, 2004 19:02 UTC Thu by d.e.cox guest, #3912 Parent article: The Grumpy Editor's Guide to PDF Viewers. Perkin Elmer GeneAmp 2400 PCR System The...
http://openwetware.org/index.php?title=User:Bill_Flanagan&diff=prev&oldid=449862
*  Cambio - Excellence in Molecular Biology
... Products. Custom Aptamer Synthesis. Cell culture contamination control. Detection. Elimination. Misc Products. Human. Detection kits and Reagents. Cytotoxicity Kits. Cytotoxicty Single Parameter Kits. Cytotoxicty Multiple Parameter Kits. DNA. Whole DNA. Sonicated DNA. DNA & RNA Isolation and purification kits. DNA. RNA. Protein. Media. DNA Ladders & Dyes. DNA ladders. DNA & RNA Sequencing. DNA Polymerases. Kits. Proteinases. Sequencing Utilities. Next Generation Sequencing. Sequencing Sample Prep Kits. Enzymes for Molecular Biology. Enzymes for Molecular Biology. Restriction Enzymes. Equipment. Homogenisation Equipment. Transilluminators and Light boxes. Thermal Cyclers. Vacuume Manifold. Gel Electrophoresis. Gel Electrophoresis. Nucleic Acid Ladders. Microarrays. In vitro Transcription. False negative PCR results are highly critical and might be caused by a defective PCR cycler. The Traditional Thermal Cycler Validation Kit provides temperature-sensitive PCR reactions to monitor an upper and lower tempe...
http://cambio.co.uk/1381/19/products/thermal-cycler-validation-kits/
*  U Mazza - ResearchGate
... For full functionality of ResearchGate it is necessary to enable JavaScript. Here are the instructions how to enable JavaScript in your web browser. U Mazza. Università degli Studi di Torino, Torino, Piedmont, Italy. Are you U Mazza. Claim your profile. Publications 114 420.48 Total impact. Article:. Distribution of Kaposi's sarcoma herpesvirus sequences among lymphoid malignancies in Italy and Spain. Cristina Pastore. Annunziata Gloghini. Gisella Volpe. Josep Nomdedeu. Eugenio Leonardo Umberto Mazza. Giuseppe Saglio. Antonino Carbonb. Gianluca Gaidano. ABSTRACT: In this study we have tested the distribution of Kaposi's sarcoma herpesvirus KSHV DNA sequences throughout the spectrum of lymphoid neoplasia in Italy and Spain. 180 cases of lymphoid malignancies representative of the major histologic and immunophenotypic categories of B- and T-cell tumours were analysed by means of a polymerase chain reaction-based assay. KSHV sequences were consistently absent in all categories of lymphoid malignanci...
http://researchgate.net/researcher/38344510_U_Mazza
*  Introduction To Organic Laboratory Techniques : a Microscale Approach (4TH 07 - Old Edition) by Dona
Introduction To Organic Laboratory Techniques : a Microscale Approach 4TH 07 - Old Edition by Donald L. Introduction To Organic Laboratory Techniques : a Microscale Approach 4TH 07 - Old Edition by Donald L. He is the coauthor of two organic laboratory books that include techniques and experiments: INTRODUCTION TO ORGANIC LABORATORY TECHNIQUES: A MICROSCALE APPROACH Cengage Learning, and A SMALL SCALE APPROACH TO ORGANIC LABORATORY TECHNIQUES Cengage Learning, as well as MICROSCALE AND MACROSCALE TECHNIQUES IN THE ORGANIC LABORATORY Cengage Learning, which highlights techniques to be used with a faculty member's own experiments. He is the coauthor of two organic laboratory books that include techniques and experiments: INTRODUCTION TO ORGANIC LABORATORY TECHNIQUES: A MICROSCALE APPROACH Cengage Learning, and A SMALL SCALE ARPPROACH TO ORGANIC LABORATORY TECHNIQUES Cengage Learning, as well as MICROSCALE AND MACROSCALE TECHNIQUES IN THE ORGANIC LABORATORY Cengage Learning, which highlights techniques to be use...
https://powells.com/biblio?isbn=9780495016304
*  Lysis buffer
... a lysis buffer is a buffer solution used for the purpose of lysing cells for use in molecular biology experiments that analyze the compounds of the cells e g western blot most lysis buffers contain salts e g tris hcl or edta to regulate the acidity and osmolarity of the lysate while detergents such as triton x or sds are added to break up membrane structures in studies like dna fingerprinting the lysis buffer is used for dna isolation dish soap can be used in a pinch to break down the cell and nuclear membranes allowing the dna to be released other such lysis buffers include the proprietary qiagen product buffer p ripa buffer is another commonly used lysis buffer for immunoprecipitation and general protein extraction from cells and tissues category laboratory techniques category cell biology category dna...
https://en.wikipedia.org/wiki/Lysis_buffer
*  METHODS FOR DETERMINING A PATIENT'S SUSCEPTIBILITY OF CONTRACTING A NOSOCOMIAL INFECTION AND FOR EST
In a preferred embodiment of the method of the invention the specific reagent comprises at least one amplification primer or at least one hybridization probe specific to the expression product of the S100A9 target gene and/or of the S100A8 target gene; or at least one antibody specific to the expression product of the S100A9 target gene and/or of the S100A8 target gene. The reagent can comprise at least one hybridization probe specific to the S100A9 target gene, at least one hybridization probe specific to the S100A8 target gene, at least one amplification primer specific to the S100A9 target gene, at least one amplification primer specific to the S100A8 target gene, at least one antibody specific to the molecule S100A9 or at least one antibody specific to the molecule S100A8. In a preferred embodiment of the method of the invention the specific reagent comprises at least one amplification primer or at least one hybridization probe specific to the expression product of the S100A9 target gene and/or of the S10...
http://freepatentsonline.com/y2011/0250592.html
*  Ready-to-Use Mixes, Standard PCR, Molecular Biology, Products & Ordering, Jena Bioscience
Ready-to-Use Mixes, Standard PCR, Molecular Biology, Products Ordering, Jena Bioscience. Contact Contact us How to find us Jena Bioscience at Meetings and Conferences. Products Ordering. Standard PCR. Your are here: Products Ordering / Molecular Biology / Standard PCR / Ready-to-Use Mixes. Standard PCR - Ready-to-Use Mixes. Ready-to-Use Mixes. Ready-to-Use Mixes. Ready-to-Use Mixes contain all reagents required for PCR except template and primer in a premixed 5x concentrated solution in one tube. Ready-to-Use Mixes for direct gel loading additionally contain an inherent red dye. PCR reaction products are thus ready-to-load onto agarose or acrylamide gels. Red Load Taq Master Master mix for direct gel loading. Product Cat. Amount Price EUR Buy / Note. S pack. PCR-108S. 1 ml 5x conc. L pack. PCR-108L. 5 x 1 ml 5x conc. Red Load Taq Master / high yield Master mix for direct gel loading. Product Cat. Amount Price EUR Buy / Note. S pack. PCR-106S. 1 ml 5x conc. L pack. PCR-106L. 5 x 1 ml 5x conc. Store at 2 to 8°C...
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*  Whitepapers from Roche Diagnostics
... White Paper Roche Diagnostics. 26-Sep-2007 Transfection of cells is one of the main techniques used to influence gene expression. Real-Time Multiplex PCR of five Different DNA Targets Using the LightCycler® 480 System. Mutation Scanning of the Cytidine Deaminase Gene by High-Resolution Melting Curve Analysis Using the LightCycler® 480 System. 05-Sep-2007 The Genome Sequencer FLX System from 454 Life Sciences and Roche Applied Science is a versatile sequencing platform suitable for a wide range of applications, including de novo sequencing and assembly of genomic DNA, transcriptome sequencing, small R more. Real-Time PCR Quantification of Plant miRNAs Using Universal ProbeLibrary Technology. Real-Time PCR Quality Control for Gene Expression. Profiling Using the LightCycler® 480 System 28-Aug-2007 Quantitative real-time PCR qPCR has become the de facto standard for nucleic acid quantification. New Application for the LightCycler® 480 System: Gene Scanning by High-Resolution Melting. 28-Aug-2007 High-resolu...
http://analytica-world.com/en/whitepapers/companies/roche-diagnostics/
*  BME103:T130 Group 17 l2 - OpenWetWare
... BME103:T130 Group 17 l2 From OpenWetWare Difference between revisions Jump to: navigation, search Revision as of 00:56, 29 November 2012 view source Ricardo Robles Talk. Revision as of 00:57, 29 November 2012 view source Ricardo Robles Talk. + + ] ] !--- Include an illustration that shows how your system's primers allow specific amplification of the disease-related SNP ---. !--- Include an illustration that shows how your system's primers allow specific amplification of the disease-related SNP ---. Lab Write-Up 1. Lab Write-Up 2. Lab Write-Up 3. 1 GROUP 17 2 LAB 2 WRITE-UP: Redesigning Open PCR for Autism Detection 2.1 Thermal Cycler Engineering 2.2 Protocols 2.3 Research and Development. Key Features The first picture shows the heating block that is in use in the current PCR machine. The picture below is that of the current cooling system. Instructions 1 Remove the current heating block and cooling system from the PCR machine. 2 Replace removed items with the larger heating block and larger cooling syst...
http://openwetware.org/index.php?title=BME103:T130_Group_17_l2&diff=660468&oldid=660466
*  Search results for: 'DISC1' - Aviva Systems Biology
Disease Categories. DISC1 antibody - C - terminal region OAAB11126 Protein Name: Disrupted in schizophrenia 1 protein Catalog #: OAAB11126 Type: pAb Reacts with: Human Application: WB, FC, IHC Product Size: 400ul Price USD : $289.00. DISC1 Antibody OAPB00558 Protein Name: Translin-associated protein X Catalog #: OAPB00558 Type: pAb Reacts with: Human; Mouse Application: ELISA, WB, ICC Product Size: 0.1 mg Price USD : $375.00. DISC1 antibody - N-terminal region ARP47934 P050 Protein Name: Disrupted in schizophrenia 1 protein Catalog #: ARP47934 P050 Type: pAb Reacts with: Horse; Human; Mouse; Rabbit; Rat Application: WB Product Size: 100 ul Price USD : $289.00. TSNAX antibody - N - terminal region OAAB10245 Protein Name: Translin-associated protein X Catalog #: OAAB10245 Type: pAb Reacts with: Human; Mouse; Rat Application: WB, IHC Product Size: 400ul Price USD : $289.00. DISC1 antibody OAAB07113 Protein Name: Disrupted in schizophrenia 1 protein Catalog #: OAAB07113 Type: mAb Reacts with: Human Application: W...
http://avivasysbio.com/catalogsearch/result/?q=DISC1&ra=146&re=Zebra Fish&pt=77
*  Search results for: 'DISC1' - Aviva Systems Biology
Disease Categories. DISC1 antibody - C - terminal region OAAB11126 Protein Name: Disrupted in schizophrenia 1 protein Catalog #: OAAB11126 Type: pAb Reacts with: Human Application: WB, FC, IHC Product Size: 400ul Price USD : $289.00. DISC1 Antibody OAPB00558 Protein Name: Translin-associated protein X Catalog #: OAPB00558 Type: pAb Reacts with: Human; Mouse Application: ELISA, WB, ICC Product Size: 0.1 mg Price USD : $375.00. DISC1 antibody - N-terminal region ARP47934 P050 Protein Name: Disrupted in schizophrenia 1 protein Catalog #: ARP47934 P050 Type: pAb Reacts with: Horse; Human; Mouse; Rabbit; Rat Application: WB Product Size: 100 ul Price USD : $289.00. DISC1 antibody OAAB07113 Protein Name: Disrupted in schizophrenia 1 protein Catalog #: OAAB07113 Type: mAb Reacts with: Human Application: WB Product Size: 400ul Price USD : $289.00. DISC1 Antibody OAPB00557 Protein Name: Translin-associated protein X Catalog #: OAPB00557 Type: pAb Reacts with: Human; Mouse Application: ELISA, WB Product Size: 0.1 mg Pr...
http://avivasysbio.com/catalogsearch/result/?q=DISC1&ra=146&single=d&ho=Rabbit
*  The Methods January 1992 Archive by author
Ericomp thermal cycler BACKD at QUCDN.QueensU.CA Multi bin feeder BIOCUKM at OSUCC.bitnet PCR/Sequencing Kit from ABI BMYCHERN at imb.as.tw Tet selection problems in pBR322 Michael Benedik Gene Fusions Michael Benedik PCR Hirdeypal S. Anthony Davies Promega Magic PCR Preps kit Dan Diaz Semipreparative 10's of micrograms PCR Dan Diaz Taq Ligase and LDR Dan Diaz gel dryer. Paul Hengen Cloning PCR products Brian Hjelle Hirt Supernatents for Cloning Viral DNA Brian Hjelle PCR Mutagenesis -Replacement of 5 bp Brian Hjelle PCR with primers of different lengths IO00865 at MAINE.MAINE.EDU microtube homogeniser. Muriana Tet selection problems in pBR322 John Nash Increasing PCR specificity with formamide John Nash Cloning PCR products Pamela Norton Subscription to Methods F. Todd Richmond System7 and Laser Question Bruce Roe Vector Sequences in the Databases Bruce Roe PCR/Sequencing Kit from ABI Bruce Roe Ericomp Thermal Cyclers Dan Rohwer-Nutter Ericomp favorable opinion. Smith Cloning PCR products Michael W. Templeto...
http://bio.net/bionet/mm/methods/1992-January/author.html
*  CST - SimpleChIP® Human NR4A3 Promoter Primers
... Related Products. SimpleChIP ® Enzymatic Chromatin IP Kit Agarose Beads. SimpleChIP ® Enzymatic Chromatin IP Kit Magnetic Beads. PRINT SimpleChIP ® Human NR4A3 Promoter Primers #4829. SimpleChIP ® Human NR4A3 Promoter Primers were tested on DNA isolated from cross-linked cells using the SimpleChIP ® Enzymatic Chromatin IP Kit Magnetic Beads #9003. Real-time PCR was performed in duplicate on a serial dilution of 2% total input DNA 20 ng, 4 ng, 0.8 ng, and 0.16 ng using a real-time PCR detection system and SYBR ® Green reaction mix. PCR product melting curves were obtained on Real-Time PCR reactions performed using SimpleChIP ® Human NR4A3 Promoter Primers. Data is shown for both duplicate PCR reactions using 20 ng of total DNA. Gallery: SimpleChIP ® Human NR4A3 Promoter Primers #4829. SimpleChIP ® Human NR4A3 Promoter Primers were tested on DNA isolated from cross-linked cells using the SimpleChIP ® Enzymatic Chromatin IP Kit Magnetic Beads #9003. Real-time PCR was performed in duplicate on a serial dilut...
http://cellsignal.com/products/chip-kits-reagents/human-nr4a3-promoter-primers/4829?N=4294967123&Nrpp=30&No=30&fromPage=plp
*  Molecular Methods for Virus Detection | 978-0-12-748920-9 | Elsevier
Molecular Methods for Virus Detection. Elsevier. Molecular Methods for Virus Detection Edited by Danny Wiedbrauk, William Beaumont Hospital, Royal Oak, Missouri, U.S.A. Daniel Farkas, William Beaumont Hospital, Royal Oak, Michigan, U.S.A. Molecular diagnostic procedures have been described in a number of recent books and articles. However, these publications have not focused on virus detection, nor have they provided practical protocols for the newer molecular methods.Written by the inventors or principal developers of these technologies, Molecular Methods for Virus Detection provides both reviews of individual methods and instructions for detecting virus nucleic acid sequences in clinical specimens. Each procedure includes quality assurance protocols that are often ignored by other methodology books. Molecular Methods for Virus Detection provides clinically relevant procedures for many of the newer diagnostic methodologies. Audience Researchers, graduate students, technicians, and clinicians in virology, mic...
http://elsevier.com/books/molecular-methods-for-virus-detection/wiedbrauk/978-0-12-748920-9
*  DNAase step in differential display PCR
... william sun william at neuro usc edu fri jun est previous message synthesis method for thiazole orange next message dnaase step in differential display pcr messages sorted by after isolation of mrna for differential display pcr is it necessary to treat the mrna with dnaase i would like to omit extra steps if possible to prevent rnaase contamination william william sun ph d phone neuroscience program fax university of southern california pager los angeles ca http rana usc edu wisun previous message synthesis method for thiazole orange next message dnaase step in differential display pcr messages sorted by more information about the methods mailing list...
http://bio.net/bionet/mm/methods/1996-June/045453.html
*  Supplements, Standard PCR, Molecular Biology, Products & Ordering, Jena Bioscience
Supplements, Standard PCR, Molecular Biology, Products Ordering, Jena Bioscience. Standard PCR. Your are here: Products Ordering / Molecular Biology / Standard PCR / Supplements. Standard PCR - Supplements. Standard PCR Supplements. Gel Loading Buffer with DNA Stain Loading buffer for agarose or polyacrylamide gels with EvaGreen fluorescent DNA stain. Product Cat. Amount Price EUR Buy / Note. PCR-255-or. Gel Loading Buffers with DNA Stain are formulated to facilitate loading of DNA samples into the wells of agarose and polyacrylamide gels. The loading buffers contain EvaGreen ® DNA Stain a fluorescent DNA intercalator dye specially developed for DNA analysis applications. Gel Loading Buffer Loading buffer for agarose or polyacrylamide gels. Product Cat. Amount Price EUR Buy / Note. PCR-254-or. Gel Loading Buffers are formulated to facilitate loading of DNA containing samples into the wells of agarose and polyacrylamide gels. Green Gel Loading Buffer contains orange G and xylene cyanol FF as tracking dyes and ...
http://jenabioscience.com/cms/sid-61f7b6556b8d4d2c2ab733f2196dbca2/1/catalog/712/?m=1&k=8e
*  Supplements, Standard PCR, Molecular Biology, Products & Ordering, Jena Bioscience
Supplements, Standard PCR, Molecular Biology, Products Ordering, Jena Bioscience. Standard PCR. Your are here: Products Ordering / Molecular Biology / Standard PCR / Supplements. Standard PCR - Supplements. Standard PCR Supplements. Gel Loading Buffer with DNA Stain Loading buffer for agarose or polyacrylamide gels with EvaGreen fluorescent DNA stain. Product Cat. Amount Price EUR Buy / Note. PCR-255-or. Gel Loading Buffers with DNA Stain are formulated to facilitate loading of DNA samples into the wells of agarose and polyacrylamide gels. The loading buffers contain EvaGreen ® DNA Stain a fluorescent DNA intercalator dye specially developed for DNA analysis applications. Gel Loading Buffer Loading buffer for agarose or polyacrylamide gels. Product Cat. Amount Price EUR Buy / Note. PCR-254-or. Gel Loading Buffers are formulated to facilitate loading of DNA containing samples into the wells of agarose and polyacrylamide gels. Green Gel Loading Buffer contains orange G and xylene cyanol FF as tracking dyes and ...
http://jenabioscience.com/cms/sid-87b41f021e15955ec8f65a6d7fa86023/1/catalog/712/?m=1&k=39
*  MassTag-PCR
masstag pcr masstag pcr masstag pcr is a new technology pcr based on mass spectrometric detection of end product this technology is pioneered by scientist from center for infection and immunity cii of mailman school of public health at columbia university usa principles like conventional pcr masstag pcr uses primer pairs the difference is primers used for masstag pcr are tagged with molecules of known masses or masscodes instead of single pair of primers this technology uses a number of primers making it a multiplex system unlike conventional multiplex pcr system in masstag pcr more than primer pairs could be used if dna from any of the agent of primer panel is present it will be amplified each amplified product will carry its specific masscodes the pcr product is then purified to remove unbound primers dntps enzyme and other impurities finally the purified pcr products are subject of uv as the chemical bond with nucleic acid and primers are photolabile as the masscodes are liberated from pcr products they ar...
https://en.wikipedia.org/wiki/MassTag-PCR
*  Healthy Supplements | Organic Supplements
Healthy Supplements. Organic Supplements. JavaScript seem to be disabled in your browser. You must have JavaScript enabled in your browser to utilize the functionality of this website. Organic Kingdom. Your Account. Your Wishlist. Contact Us. Shopping Cart. Checkout. Login. Home /. Supplements. Supplements. Items 10 to 18 of 428 total Show. 9. 15. 30. All. per page Page:. 1 2. 3 4. 5. View as: List Grid Sort By. Position. Name. Price. Klamath Shores Blue Green Algae Product Size: 60 caps $15.99. Add to Cart. Add to Wishlist. Add to Compare. More Than A Multiple Product Size: 120 tabs $29.50. Add to Cart. Add to Wishlist. Add to Compare. Potent Acidophilus Supplement Product Size: 100 caps $9.50. Add to Cart. Add to Wishlist. Add to Compare. Royal Brittany Evening Primrose Oil Product Size: 100 softgels + $16.99. Add to Cart. Add to Wishlist. Add to Compare. Salmon Oil Product Size: 120 softgels $7.99. Add to Cart. Add to Wishlist. Add to Compare. American Native Herbal TABLETS Product Size: 60 tabs $55.99. Ad...
http://organickingdom.com/supplements.html?dir=asc&limit=9&mode=grid&order=position&p=2
*  In Their Own Words
... Office of NIH History. Polymerase Chain Reaction Test. Diagram of the polymerase chain reaction test PCR shows how HIV DNA sequences as a way to diagnose HIV infection in blood samples. return. Office of NIH History. NIH. DHHS....
https://history.nih.gov/NIHInOwnWords/docs/page_39g.html
*  Applications of PCR
... ::'This page assumes familiarity with the terms and components used in the polymerase chain reaction PCR process. The polymerase chain reaction PCR has found widespread application in many areas of genetic analysis. Medical applications Infectious disease applications Forensic applications Research applications External links References. Medical applications. was for ' genetic testing ', where a sample of DNA is analyzed for the presence of genetic disease mutations. Prospective parents can be tested for being genetic carrier s, or their children might be tested for actually being affected by a disease. PCR analysis is also essential to preimplantation genetic diagnosis, where individual cells of a developing embryo are tested for mutations. Infectious disease applications. Characterization and detection of infectious disease organisms have been revolutionized by PCR:. The earliest tests for infection relied on the presence of antibodies to the virus circulating in the bloodstream. However, antibodies do...
https://en.wikipedia.org/wiki/Applications_of_PCR
*  KI and DNA purification
... dr duncan clark duncan at nospam demon co uk tue mar est previous message ki and dna purification next message ki and dna purification messages sorted by in article bpmurray stuffer at macmac ucsf edu bernard p murray phd bpmurray stuffer socrates ucsf edu writes i have inherited a protocol for purifying dna from agarose gels with promega s agarace enzyme the protocol i have calls for melting the gel slices in m ki final conc m in ul total volume before adding the agarace thanks jennifer is that really ki i thought most protocols user periodate kio or perchlorate for dissolution of agarose sorry to question you its just that the idea of using ki is new to me don t know about ki but m nai with g l na sulphite works fine store in the dark or an opaque bottle etc if it is stored in the light it will go yellow duncan the problem with being on the cutting edge is that you occasionally get sliced from time to time duncan clark dnamp ltd tel fax http www dnamp com http www genesys demon co uk previous message k...
http://bio.net/bionet/mm/methods/1999-March/074628.html
*  Videos for polymerase chain reaction - Homework Help Videos - Brightstorm
videos for polymerase chain reaction homework help videos brightstorm toggle navigation browse subjects math pre algebra algebra geometry algebra trigonometry precalculus calculus science biology chemistry physics english grammar writing literature test prep sat act act red book psat ap us gov ap us history ap biology ap calculus ab college get better grades college application college essay financial aid search or find by textbook study math science english test prep sign in teacher membership school membership start your free trial videos for polymerase chain reaction pcr dna fingerprinting science biology molecular biology the pcr method of dna fingerprinting tags pcr dna fingerprinting gel electrophoresis polymerase chain reaction about how to use about us our teachers for schools jobs press webinars ebooks support faq library math pre algebra algebra geometry algebra trigonometry precalculus calculus science biology chemistry physics english grammar writing literature test prep sat act act red book psat ...
http://brightstorm.com/tag/polymerase-chain-reaction/
*  Search results for: 'SLC2A5' - Aviva Systems Biology
... Polyclonal Antibodies. Cell Cycle Proteins Antibodies. Membrane Protein Antibodies. RNA Binding Proteins Antibodies. Membrane Protein Antibodies. Protein. DNA/cDNA/RNA. Kits. ELISA Kits. Other Kits. RT PCR Pairs. Tissues Cells. By Gene. Apoptosis. Cancer. Cell Biology. Chromatin Nuclear Signaling. Disease Related. DNA Damage Repair. DNA Repair. DNA/RNA/Protein Interactions. Stem Cells. Transcription Factors. Disease Categories. Filter by Product Name: Show All A B C D E F G H I J K L M N O P Q-R S T U V W Y Z 0-1 2-4 5 6 7-9. SLC2A5 Antibody ARP42096 P050 Protein Name: Solute carrier family 2, facilitated glucose transporter member 5 Catalog #: ARP42096 P050 Type: pAb Reacts with: Cow; Dog; Guinea Pig; Horse; Human; Mouse; Rat; Sheep Application: IF, WB Product Size: 100 ul Price USD : $289.00. GLUT5 Polyclonal Antibody OABB00689 Protein Name: Solute carrier family 2, facilitated glucose transporter member 5 Catalog #: OABB00689 Type: pAb Reacts with: Human; Mouse; Rat Application: WB IHC-P Product Size:...
http://avivasysbio.com/catalogsearch/result/?q=SLC2A5&pt=47&cl=Monoclonal
*  relative expression of genes using semiquantitative PCR - PCR, RT-PCR and Real-Time PCR - BioForum
... Google Sign in options. Remember me This is not recommended for shared computers Sign in anonymously Don't add me to the active users list Privacy Policy. Sign In. Create Account. This topic. Forums. Members. BioBlog. BioWiki. Calendar. Quotes. BioVideo. Forums. BioBlog. BioWiki. Calendar. Quotes. Contact Us. BioForum. Protocols and Techniques Forums. PCR, RT-PCR and Real-Time PCR. Javascript Disabled Detected You currently have javascript disabled. relative expression of genes using semiquantitative PCR Started by. Please log in to reply. 1 reply to this topic #1. nirupakl. nirupakl. member Active Members. 11 posts. First of all would like to apolgise for posting a question on semi-quantitative PCR in the era of real-time PCR. I have analyzed expression of three genes: a, b and c in certain pathological tissues. I have performed agarose gel electrophoresis and have the gels analyzed by Bio-Rad gel documentation system. i have seen some papers which say that they have seen relative expression with respec...
http://protocol-online.org/forums/topic/21976-relative-expression-of-genes-using-semiquantitative-pcr/
*  CST - SimpleChIP® Mouse MYT-1 Promoter Primers
... Related Products. SimpleChIP ® Enzymatic Chromatin IP Kit Agarose Beads. SimpleChIP ® Enzymatic Chromatin IP Kit Magnetic Beads. PRINT SimpleChIP ® Mouse MYT-1 Promoter Primers #8985. Products. SimpleChIP ® Mouse MYT-1 Promoter Primers were tested on DNA isolated from cross-linked cells using the SimpleChIP ® Enzymatic Chromatin IP Kit Magnetic Beads #9003. Real-time PCR was performed in duplicate on a serial dilution of 2% total input DNA 20 ng, 4 ng, 0.8 ng, and 0.16 ng using a real-time PCR detection system and SYBR ® Green reaction mix. PCR product melting curves were obtained for real-time PCR reactions performed using SimpleChIP ® Mouse MYT-1 Promoter Primers. Data is shown for both duplicate PCR reactions using 20 ng of total DNA. Gallery: SimpleChIP ® Mouse MYT-1 Promoter Primers #8985. SimpleChIP ® Mouse MYT-1 Promoter Primers were tested on DNA isolated from cross-linked cells using the SimpleChIP ® Enzymatic Chromatin IP Kit Magnetic Beads #9003. Real-time PCR was performed in duplicate on a s...
http://cellsignal.com/products/cellular-assay-kits/8985.html
*  Genetic analysis
Basic studies include identification of genes and inherited disorders. Genetic analysis can be used generally to describe methods both used in and resulting from the sciences of genetics and molecular biology, or to applications resulting from this research. Genetic analysis may be done to identify genetic/inherited disorders and also to make a differential diagnosis in certain somatic diseases such as cancer. Genetic analyses of cancer include detection of mutation s, fusion gene s, and DNA copy number changes. 'Genetic analyses' include but are not limited to molecular technologies such as PCR, RT-PCR, DNA sequencing, and DNA microarrays, and cytogenetic methods such as karyotyping and fluorescence in situ hybridisation. DNA Sequencing. DNA sequencing is essential to the applications of genetic analysis. 'Cytogenetics' is a branch of genetics that is concerned with the study of the structure and function of the cell, especially the chromosomes Polymerase chain reaction studies the amplification of DNA. Poly...
https://en.wikipedia.org/wiki/Genetic_analysis
*  Primers PCR-Vectorette.
primers pcr vectorette primers pcr vectorette jingfang niu jniu at nmu edu wed nov est previous message oligo program next message chloroform in colony blots messages sorted by i am also trying to use the vectorette ii kit by genosys i believe genosys patented the vectorette system therefore they don t divulge the primer or vectorette sequence information if they had i would have been able to construct my own vectorette units and primers vs the universal primer listed anywhere i have already constructed my own vectorette library however i have been getting strange results so i opted to purchase a genosys kit you can run a control using only primers if you are looking for primer dimers last time i ran a vectorette reaction i ran control reactions using single primers and no template reactions surprisingly enough i got a product using only one primer my specific primer this may be due to some inversion or repeated sequences if you have any luck using vectorette ii please let me know it seems to be a tricky syst...
http://bio.net/bionet/mm/methods/1998-November/071933.html
*  Aviva Systems Biology - Reagents & Antibodies
CDK5 antibody - N-terminal region ARP38525 T100 Protein Name: Cyclin-dependent kinase 5 Catalog #: ARP38525 T100 Type: pAb Reacts with: Cow; Dog; Guinea Pig; Horse; Human; Mouse; Rabbit; Rat; Sheep; Zebrafish Application: WB Product Size: 100 ul Price USD : $229.00. CDK5 antibody - C-terminal region ARP38526 T100 Protein Name: Cyclin-dependent kinase 5 Catalog #: ARP38526 T100 Type: pAb Reacts with: Cow; Dog; Guinea Pig; Horse; Human; Mouse; Pig; Rabbit; Rat; Sheep; Yeast; Zebrafish Application: IHC, WB Product Size: 100 ul Price USD : $229.00. NCOR1 antibody - N-terminal region ARP32479 P050 Protein Name: Nuclear receptor corepressor 1 Catalog #: ARP32479 P050 Type: pAb Reacts with: Cow; Dog; Guinea Pig; Horse; Human; Mouse; Rat; Yeast Application: IHC, WB Product Size: 100 ul Price USD : $289.00. Foxa3 antibody - C-terminal region ARP36881 P050 Protein Name: Hepatocyte nuclear factor 3-gamma Catalog #: ARP36881 P050 Type: pAb Reacts with: Cow; Dog; Guinea Pig; Horse; Human; Mouse; Pig; Rat Application: WB P...
http://avivasysbio.com/catalogsearch/advanced/result/?related_component=Nuclear Part
*  DNA Isolation
... Entamoeba is known to store glycogen and standard DNA isolation methods often yield DNA that is difficult to digest with restriction enzymes or use as a template for polymerase chain reaction. Sample collection My own experience has been only with material from in vitro cultures so that readers may want to find methods for DNA isolation directly from stool or other sources elsewhere. However, I know from work by other researchers that this method has been used successfully to isolate Entamoeba DNA from stool that was a suitable template for Polymerase Chain Reaction amplification. 1 A pellet of at least 50 l packed volume can be processed with the following protocol. Add 75 l of 3.5 M NaCl, mix gently then add 42 l of 10% CTAB/0.7 M NaCl Note heated to 55 C, mix and incubate at 65 C for 20 minutes. At room temperature add 400 l of chloroform, mix well by inverting and spin at full speed in a microcentrifuge for 10 minutes. Note. Transfer the supernatant to a fresh tube and add 400 l of phenol:chloroform:...
http://entamoeba.lshtm.ac.uk/dnaisoln.htm
*  Molecular Biology, Products & Ordering, Jena Bioscience
Standard PCR. Reverse Transcription. Ready-to-Use Lyophilisates. DNA Ladders. Protein MW Marker / Blot stain. Cloning and Mutagenesis. Thermophilic Polymerases dNTP Guide qPCR with EvaGreen RNA / DNA Purification DNA Ladders Guide Restr. Standard PCR Direct and Multiplex PCR Ready-to-Use Mixes Core Kits Thermophilic Polymerases dNTP Mixes dNTP Bundles and Single Solutions Supplements School and Demo Kits Real-Time PCR qPCR Multiplex Master Mixes qPCR Master Mixes for Dual Labeled Probes qPCR Master Mixes with EvaGreen qPCR Core Kits qPCR Supplements Dual Labeled Fluorescent Probes Reverse Transcription One-Step RT-qPCR One-Step RT-PCR Two-Step RT-PCR Reverse Transcriptases Supplements in vitro Transcription in vitro Transcription Kits RNA Polymerases NTP Solutions Ready-to-Use Lyophilisates Real-Time Master Lyophilisates Direct PCR and Multiplex PCR Lyophilisates Taq Hot Start Master Lyophilisates Reverse Transcription Master Lyophilisates dNTP Lyophilisates Custom-Specific Lyophilizates Primers and Oligonucl...
http://jenabioscience.com/cms/sid-c0cf6056b1bcda3eb9ad4ee21708c41c/1/browse/109_molecular_biology.html
*  Molecular Biology, Products & Ordering, Jena Bioscience
Standard PCR. Reverse Transcription. Ready-to-Use Lyophilisates. DNA Ladders. Protein MW Marker / Blot stain. Cloning and Mutagenesis. Thermophilic Polymerases dNTP Guide qPCR with EvaGreen RNA / DNA Purification DNA Ladders Guide Restr. Standard PCR Direct and Multiplex PCR Ready-to-Use Mixes Core Kits Thermophilic Polymerases dNTP Mixes dNTP Bundles and Single Solutions Supplements School and Demo Kits Real-Time PCR qPCR Multiplex Master Mixes qPCR Master Mixes for Dual Labeled Probes qPCR Master Mixes with EvaGreen qPCR Core Kits qPCR Supplements Dual Labeled Fluorescent Probes Reverse Transcription One-Step RT-qPCR One-Step RT-PCR Two-Step RT-PCR Reverse Transcriptases Supplements in vitro Transcription in vitro Transcription Kits RNA Polymerases NTP Solutions Ready-to-Use Lyophilisates Real-Time Master Lyophilisates Direct PCR and Multiplex PCR Lyophilisates Taq Hot Start Master Lyophilisates Reverse Transcription Master Lyophilisates dNTP Lyophilisates Custom-Specific Lyophilizates Primers and Oligonucl...
http://jenabioscience.com/cms/sid-c5378fbb19c61c10c5efad01ba67454b/1/browse/109_molecular_biology.html
*  Licensing your thermocycler for PCR?
licensing your thermocycler for pcr licensing your thermocycler for pcr wschick at aol com wschick at aol com thu oct est previous message vogel bonner next message optimal running time for sscp messages sorted by i don t know of any cycler manufacturer that can license for diagnostic purposes only for research roche held back diagnostic licensing market from pe but now pe applied biosystems has announced an agreement from roche to use pcr with their fluorescent technology i guess that s taqman you should contact roche about diagnostic licensing or pcr you could start at roche molecular in alameda california ph walt schick i have no connection to roche or pe abi previous message vogel bonner next message optimal running time for sscp messages sorted by more information about the methods mailing list...
http://bio.net/bionet/mm/methods/1996-October/050917.html
*  What Assays are used in Protein Concentration Determination?
What Assays are used in Protein Concentration Determination. Protein Electrophoresis 17. Protein Purification 16. Protein Estimation 12. Protein Extraction 11. Sample Clean Up 9. Protein Concentration 8. Protein Detection 7. Assay Development ELISA 6. Apoptosis Assays 5. Protein Fractionation 4. Why do I need a protease inhibitor. Using Protease Assays for Accurate Protease Detection. Are Total Protein Membrane Stains Compatible With IR Imaging Systems. What Assays are used in Protein Concentration Determination. What assays are used in protein concentration determination. Due to the fact that there are no protein assays that are specific to any particular protein or are sensitive to all types of proteins, it is absolutely impossible to have a single assay that can give absolutely accurate results when performing protein concentration determination. To find protein assays that are most compatible with your sample, you need to consider their compatibility with the sample type and its components, the assay rang...
http://info.gbiosciences.com/blog/bid/163070/What-Assays-are-used-in-Protein-Concentration-Determination
*  The Secrets of Coupling with Biotin!
Protein Research. Protein Electrophoresis 17. Protein Purification 16. Protein Estimation 12. Protein Extraction 11. Protein Detection 7. Why do I need a protease inhibitor. Using Protease Assays for Accurate Protease Detection. Are Total Protein Membrane Stains Compatible With IR Imaging Systems. The conjugation efficiency of the reactions is dependent on the reaction groups and the buffers used for the reactions as many coupling reactions are sensitive to pH and chemical composition. Reaction Conditions. NHS esters are soluble in organic solvents and DMSO or DMF are the most commonly used, which are compatible with most proteins in a 20% solution. For optimal conjugation, we recommend Optimizer Buffer™-I. General Precautions. There are three different reactions employed to couple biotin reagents to sulfhydryl residues and involve either iodoacetyl, maleimide or pyridylthiol groups. Maleimide Reaction Conditions. The maleimide group is more specific for sulfhydryl residues than iodoacetyl groups, at pH7 male...
http://info.gbiosciences.com/blog/bid/128778/the-secrets-of-coupling-with-biotin
*  AP-PCR QUESTION
ap pcr question ap pcr question jenny williams jenova at microbes demon co uk sun jun est previous message postdoctoral position next message respirometer for measuring o co of bacteria algae messages sorted by i have recently completed a project on molecular typing using arbitrary primer pcr ap pcr this technique uses a single arbitrary primer in each pcr assay i used random primers based on published sequences which had previously been applied successfully in the typing of clostridium difficile i followed the published protocols carefully but was unable to get reproducible results i then combined the same two random primers that i had previously used in single primer pcr assays in the same reaction tube the assay conditions were identical to that of the single primer assays with the exception that primers were now taking part in the reaction also the total amount of primer in the reaction tube was doubled this combination produced reproducible results also the resulting dna profile produced after gel electr...
http://bio.net/bionet/mm/methods/1997-June/058932.html
*  PCR Techniques & Applications Workshop
Previous message: rDNA Techniques Applications Workshop Next message: gel doc/photo imaging system Messages sorted by:. POLYMERSE CHAIN REACTION PCR APPLICATIONS/ CYCLE DNA SEQUENCING. American Type Culture Collection ATCC April 21-24, 1997, Rockville, MD A four-day, laboratory-intensive, course covering a broad range of applications of the polymerase chain reaction PCR for addressing biological problems. Lecture topics will include PCR Reaction Basics, Optimizing and Troubleshooting the PCR Reaction, DNA Sequencing, PCR Analysis of Ribosomal DNA to Determine Taxonomic Relatedness, PCR Fingerprinting, PCR in the Diagnosis of Infectious and Genetic Disease, and PCR and Recombinant DNA Methodology. Laboratory exercises will include: 1 PCR Diagnosis of Sickle Cell Disease; 2 Fingerprint Analysis of the DNA of Human Individuals; 3 Relatedness of Organisms by Analysis of Amplified Ribosomal DNA; 4 Amplification from mRNA; 5 Optimization of PCR Reactions; 6 Labeling of Hybridization Probes by PCR; 7 Cycle DNA Seque...
http://bio.net/bionet/mm/methods/1997-March/055281.html
*  Lysis Buffer for Total Protein Assay - Protein Expression and Purification - BioForum
... Jump to content. Google Sign in options. Remember me This is not recommended for shared computers Sign in anonymously Don't add me to the active users list Privacy Policy. . Sign In. Create Account. Search Advanced. Search section:. This topic. Forums. Members. Help Files. BioBlog. BioWiki. Interest Groups. Calendar. Quotes. BioVideo. View New Content. Protocol Online. BioPortal. Forums. BioBlog. BioWiki. Interest Groups. Calendar. Quotes. Contact Us. More. BioForum. Protocols and Techniques Forums. Protein Expression and Purification. . Javascript Disabled Detected You currently have javascript disabled. Several functions may not work. Please re-enable javascript to access full functionality. Submit your paper to J Biol Methods today. Lysis Buffer for Total Protein Assay Started by. Lupo Comunitario, Apr 08 2009 04:54 AM. Please log in to reply. 1 reply to this topic #1. Lupo Comunitario. Lupo Comunitario. member Members. 3 posts. 1 Neutral. Posted 08 April 2009 - 04:54 AM Dear All I need to measure the...
http://protocol-online.org/forums/topic/7413-lysis-buffer-for-total-protein-assay/
*  Looking for the optimal Cell Lysis Buffer
... Lahti via methods%40net.bio.net by laht0028 from umn.edu Mon Dec 10 13:12:44 EST 2007. Previous message: how to calculate Kcat value of enzyme Next message: Rhodamine dUTP and capillary eletrophoresis Messages sorted by:. I am looking for a cell lysis buffer that will help me solubilize a lysosmal cathepsin. I am currently finding the Cathepsin in my cell lysate samples as well as my cell pellet. The cathepsin may be membrane associated with the plasma membrane or other cellular membranes. What are detergents and/or buffers that might best aid me in removing a cathepsin from my cellular debris. Lahti -- View this message in context: http://www.nabble.com/Looking-for-the-optimal-Cell-Lysis-Buffer-tp14258171p14258171.html Sent from the Bio.net - Methods mailing list archive at Nabble.com. Previous message: how to calculate Kcat value of enzyme Next message: Rhodamine dUTP and capillary eletrophoresis Messages sorted by:. More information about the Methods mailing list....
http://bio.net/bionet/mm/methods/2007-December/102750.html
*  Q: Inaccuracies in PCR sequencing and genetic variability
q inaccuracies in pcr sequencing and genetic variability q inaccuracies in pcr sequencing and genetic variability fred rickson ricksonf at bcc orst edu fri feb est previous message q inaccuracies in pcr sequencing and genetic variability next message hershey chargaff messages sorted by on thu feb daniel gautheret wrote i bet this issue has already been debated but i can t find any relevant data in the litterature so here it is direct sequencing of pcr products often yields sequences with many inaccuracies ns can we say that the position of these undefined nucleotides is related to actual genetic variation at these particular sites thank you daniel gautheret i suspect that if you are getting more than an acceptable amount of misreads then you are going to have to clone what must be a heterogeneous target area on a clean dna region i can sequence times with maybe two changes each time over bases fred rickson previous message q inaccuracies in pcr sequencing and genetic variability next message hershey chargaff ...
http://bio.net/bionet/mm/bioforum/1997-February/022671.html
*  RACE pcr product - Biology Stack Exchange
... Biology Meta. more stack exchange communities. Stack Exchange. Help Center Detailed answers to any questions you might have. Biology Questions. Biology Stack Exchange is a question and answer site for biology researchers, academics, and students. RACE pcr product. If it wanted to sequence my PCR product that might be hundreds, if not thousands of bps long, I should use RACE PCR correct. My goal is to sequence the active site of some enzymes I'm interested in, but I'm not sure how to go about designing PCR primers for a specific site on my protein. Additionally, the active site is over 100 amino acids long, which would imply that my PCR product has to be over 300 bps long. I was thnking about using RACE PCR to amplify my entire cDNA strand that is 4000 bps long and then just sequencing the RACE product. Would RACE work for that many bps in my cDNA. It would probably be best if you asked separate questions. Using these flanking sequences, two short oligonucleotides e.g., 18-24 nucleotides, called primers, ...
http://biology.stackexchange.com/questions/7593/race-pcr-product/7606
*  Development and Evolution of PCR | GEN Magazine Articles | GEN
Market & Tech Analysis. PCR the enzymatic amplification of a specific DNA fragment targeted by two oligonucleotide primers is a surprisingly simple concept but a technology that has become increasingly powerful and broadly implemented. Starting with a DNA or RNA template, repeated cycles of denaturation, primer annealing, and polymerase-mediated primer extension generate an exponential accumulation of a specifc targeted fragment that can be analyzed by a variety of methods. Under these conditions, the first successful genomic PCRs resulted in dramatic amplification and enrichment of the targeted 110 bp fragment of -globin, but only around 1% of the amplified DNA was the target. By allowing primer annealing and extension at elevated temperatures, the use of a thermostable polymerase also greatly increased the specificity of target amplification. Discovery of DNA polymerases that could synthesize a cDNA strand from an RNA template made possible PCR assays for gene expression as well as detection and characteriz...
http://genengnews.com/gen-articles/development-and-evolution-of-pcr/4801/?kwrd=DNA
*  PCR product stays on agarose gel well !! - PCR, RT-PCR and Real-Time PCR
And another "theory" have you tried to clean up your PCR product column or precipitation, not enzymatic, maybe something in your PCR buffer causes "strange" running of your template. In this gel I just ran a random PCR product, probably one of the primer sets that did not work. I do get the correct band when using primers for a different gene amplicon that we use in another project and I was using here as positive control. Anyway, we had a student run a PCR will run gel today, with a plate rather than tubes like I was using, and some of my tubes also. Will know soon if there is something wrong with the tubes, or with me. And another "theory" have you tried to clean up your PCR product column or precipitation, not enzymatic, maybe something in your PCR buffer causes "strange" running of your template. mmf on Jul 1 2009, 04:31 PM said: One of my primer sets I was testing several seem to be working but I still need to optimize to minimize other bands. In this gel I just ran a random PCR product, probably one...
http://protocol-online.org/biology-forums-2/posts/8916.html
*  primers in RT in situ PCR
... rob wesley rwesley at welchlink welch jhu edu mon mar est previous message ligation of alkaline phosphatase treated dna next message primers in rt in situ pcr messages sorted by hi i m trying to work out a method for rt in situ pcr and i m at a conceptual snag in the rt step i want to use the specific downstream primer but i m not sure which one this is is it the one complementary to the end of the mrna can i use one of my pcr primers in this step would an oligo dt primer cause me all kinds of trouble how long should the rt primer be robert wesley johns hopkins university department of neurology previous message ligation of alkaline phosphatase treated dna next message primers in rt in situ pcr messages sorted by more information about the methods mailing list...
http://bio.net/bionet/mm/methods/1995-March/025568.html
*  Best DNA extraction method from BAL-supernatant
best dna extraction method from bal supernatant best dna extraction method from bal supernatant jannik helweg jhelweg at inet uni dk sat jun est previous message free antibody resource next message mushroom hunters messages sorted by looking for recommedations on the best method to extract dna from bronchoalveolar lavage bal supernatant i am doing research on p carinii at my hospital we have a large bank of bal s however as some of the cell pellets already have been used for other purposes in some instances the only material we have left is supernatant originally bal s ml was spun at g for min and supernatant approximately ml saved at c and c i have tried doing simple ethanol precipitation on the supernatants and tried a bio gnome dna isolation kit but by both methods the yield is low and pcr too often negative anybody know better methods for extracting a probably tiny amount of dna from this material thanks jannik helweg larsen dept of infectious diseases hvidovre hospital denmark e mail xxjhelweg at inet un...
http://bio.net/bionet/mm/mycology/1998-June/006751.html
*  BME103:W930 Group1 l2 - OpenWetWare
... BME103:W930 Group1 l2 From OpenWetWare Revision as of 18:49, 25 November 2012 by Brianna S. Ackerman Talk. contribs diff ←Older revision. Current revision diff. Newer revision→ diff. Jump to: navigation, search. BME 103 Fall 2012. Home. People. Lab Write-Up 1. Lab Write-Up 2. Lab Write-Up 3. Course Logistics For Instructors. Photos. Wiki Editing Help. Contents. 1 OUR TEAM 2 LAB 2 WRITE-UP 2.1 Thermal Cycler Engineering 2.2 Protocols 2.3 Research and Development. OUR TEAM. Name: Kevin Chu Experimemtal Protocol Planner. Name: Student Role s. Name: Student Role s. Name: Student Role s. Name: Student Role s. Name: Student Role s. Name: Student Role s. LAB 2 WRITE-UP. Thermal Cycler Engineering. Our re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski. System Design. Key Features. Instructions. Protocols. Materials. Supplied in the Kit. Amount. PCR Machine 1. Fluorimeter 1. Patient’s Template DNA 0.1. Positive Control TBD. Negative Control TBD. 10μM forward prime...
http://openwetware.org/index.php?title=BME103:W930_Group1_l2&oldid=658583
*  cDNA synthesis
... terry tcklau at hotmail com sat feb est previous message mol biol web site next message well genomic dna extraction techniques messages sorted by hello recently i would like to construct a cdna library after inserts were ligated into plasmid it would be transformed into e coli however the transformation efficiency is low then all the clones were proceeded mini prep and analysed by gel electrophoresis from the gel it can be seen that only few clones have inserts and the size is about k but the size of cdna is ranged from to base pair can anyone tell me what is my problem thank you in advance terry previous message mol biol web site next message well genomic dna extraction techniques messages sorted by more information about the methods mailing list...
http://bio.net/bionet/mm/methods/1999-February/073317.html
*  Laboratories
... in join sign in branchburg laboratories bio reference laboratories inc robbins rd branchburg nj honors process containment strawberry hill rd branchburg nj hydromer inc industrial pkwy branchburg nj im clone systems inc chubb way branchburg nj merck co inc river rd branchburg nj life cell corp millennium way branchburg nj im clone systems inc us highway n branchburg nj see more warren laboratories nicox inc independence blvd ste warren nj bio array solutions limited technology dr ste warren nj celgene cellular therapeutics powderhorn dr warren nj see more about us contact us phone book opt out mobile pay bill advertise national telco yellow pages white pages top cities top categories dex media all rights reserved data provided by infogroup acxiom privacy policy and terms of use advertiser login...
http://dexknows.com/local/science_and_engineering/laboratories/geo/co-somerset_county-nj/
*  PCR sequencing
... mika salminen msalminen finnphi at pucc princeton edu tue oct est previous message none next message proper posting of queries and messages messages sorted by as i think this is of general interest i reply to the net doug we at finnphi bitnet do a lot of pcr sequencing with reasonable success here s our protocol in which you may find the answears you need denature pmol of pcr product purified by heating min at c in water snap cool on ice for min add pmol primer add buffer anneal primer for min at c add enzyme label and do the labelling for min at c divide to extension termination reactions and keep at c stop reaction loading buffer there are a few more tricks if your dna is dirty as agarose purified use double amount of enzyme this countereffects the agarose inhibitory effect on enzyme activity if you want to read near the primer add manganese buffer from the newest sequenase kit one tenth of total reaction volume tabor et richardson pnas we do our sequencing using the amersham multiwell system in a mikr...
http://bio.net/bionet/mm/methods/1990-October/002758.html
*  - PCR--RT-PCR-and-Real-Time-PCR
... BioJob BioBlog PubAlert BioTool BioProduct BioForum Protocol Home. Forum Index 1999-2009 Home Forum Index 2009- Home Live Discussion. Top : New Forum Archives 2009- : : PCR--RT-PCR-and-Real-Time-PCR. 331. Scaling up PCR to get more DNA - reply: 5 332. RNA concentration suddenly drops - reply: 3 333. Problem using evagreen evagreen vs sybrgreen - reply: 4 334. PCR to get 10kbp product - reply: 4 335. site-directed mutagenesis primer Tm - reply: 4 336. Single-step nested PCR: how to investigate dynamics. - reply: 2 337. RNA concentration to start RT reaction - reply: 1 338. Random vs oligo primer in preamplification RT - reply: 1 339. Smart Cycler II software and Absolute quantification help - reply: 1 340. cDNA amount in qPCR - reply: 2 341. mRNA integrity for qPCR - reply: 6 342. Strange control contamination in cDNA synthesis - reply: 2 343. how does polymerase stop at the required length - reply: 1 344. Perfect dilution curve, but double peak melting curve on SYBR qPCR - reply: 7 345. Real-time efficie...
http://protocol-online.org/biology-forums-2/PCR--RT-PCR-and-Real-Time-PCR/more11.html
*  PCR-sequencing on colonies
pcr sequencing on colonies pcr sequencing on colonies ajwatson ajwatson at romeo caltech edu thu mar est previous message e mail address next message dopamine receptor ihc messages sorted by in article cnjbn w at biobase aau dk kjaer at biobase aau dk svend kjaer writes i would like to hear from anybody who has succesfully done pcr sequencing directly on bacteriacolonies grown on plates if anyone could advice me some literature or practical approaches i would be very thankfull i do this routinely simply touch a toohpick to the colony resuspend it ul of dh o boil it for min microfuge out the debris and use about ul or so in a ul reaction use the sequencing primers that flank the insert pcr amplify a control plasmid no insert as well one of my labmates claims to done it simply by taking part of the resusupended plasmid no boiling but i don t know about this first hand cheers john previous message e mail address next message dopamine receptor ihc messages sorted by more information about the methods mailing list...
http://bio.net/bionet/mm/methods/1994-March/012941.html

Amplified fragment length polymorphismGene polymorphismThermal cyclerWGAViewer: WGAViewer is a bioinformatics software tool which is designed to visualize, annotate, and help interpret the results generated from a genome wide association study (GWAS). Alongside the P values of association, WGAViewer allows a researcher to visualize and consider other supporting evidence, such as the genomic context of the SNP, linkage disequilibrium (LD) with ungenotyped SNPs, gene expression database, and the evidence from other GWAS projects, when determining the potential importance of an individual SNP.Restriction fragment: A restriction fragment is a DNA fragment resulting from the cutting of a DNA strand by a restriction enzyme (restriction endonucleases), a process called restriction. Each restriction enzyme is highly specific, recognising a particular short DNA sequence, or restriction site, and cutting both DNA strands at specific points within this site.Symmetry element: A symmetry element is a point of reference about which symmetry operations can take place. In particular, symmetry elements can be centers of inversion, axes of rotation and mirror planes.Coles PhillipsInfinite alleles model: The infinite alleles model is a mathematical model for calculating genetic mutations. The Japanese geneticist Motoo Kimura and American geneticist James F.Homing endonuclease: The homing endonucleases are a collection of endonucleases encoded either as freestanding genes within introns, as fusions with host proteins, or as self-splicing inteins. They catalyze the hydrolysis of genomic DNA within the cells that synthesize them, but do so at very few, or even singular, locations.DNA sequencer: A DNA sequencer is a scientific instrument used to automate the DNA sequencing process. Given a sample of DNA, a DNA sequencer is used to determine the order of the four bases: G (guanine), C (cytosine), A (adenine) and T (thymine).Amplified Ribosomal DNA Restriction Analysis: Amplified rDNA (Ribosomal DNA) Restriction Analysis is the extension of the technique of RFLP (restriction fragment length polymorphism) to the gene encoding the small (16s) ribosomal subunit of bacteria. The technique involves an enzymatic amplification using primers directed at the conserved regions at the ends of the 16s gene, followed by digestion using tetracutter Restriction enzymes.Chromosome regionsGenetic variation: right|thumbBranching order of bacterial phyla (Gupta, 2001): There are several models of the Branching order of bacterial phyla, one of these was proposed in 2001 by Gupta based on conserved indels or protein, termed "protein signatures", an alternative approach to molecular phylogeny. Some problematic exceptions and conflicts are present to these conserved indels, however, they are in agreement with several groupings of classes and phyla.DNA condensation: DNA condensation refers to the process of compacting DNA molecules in vitro or in vivo. Mechanistic details of DNA packing are essential for its functioning in the process of gene regulation in living systems.Genetic linkage: Genetic linkage is the tendency of alleles that are located close together on a chromosome to be inherited together during the meiosis phase of sexual reproduction. Genes whose loci are nearer to each other are less likely to be separated onto different chromatids during chromosomal crossover, and are therefore said to be genetically linked.Composite transposon: A composite transposon is similar in function to simple transposons and Insertion Sequence (IS) elements in that it has protein coding DNA segments flanked by inverted, repeated sequences that can be recognized by transposase enzymes. A composite transposon, however, is flanked by two separate IS elements which may or may not be exact replicas.Ligation-independent cloning: Ligation-independent cloning (LIC) is a form of molecular cloning that is able to be performed without the use of restriction endonucleases or DNA ligase. This allows genes that have restriction sites to be cloned without worry of chopping up the insert.Restriction site: Restriction sites, or restriction recognition sites, are locations on a DNA molecule containing specific (4-8 base pairs in length) sequences of nucleotides, which are recognized by restriction enzymes. These are generally palindromic sequences (because restriction enzymes usually bind as homodimers), and a particular restriction enzyme may cut the sequence between two nucleotides within its recognition site, or somewhere nearby.Community Fingerprinting: Community fingerprinting refers to a set of molecular biology techniques that can be used to quickly profile the diversity of a microbial community. Rather than directly identifying or counting individual cells in an environmental sample, these techniques show how many variants of a gene are present.Nested case-control study: A nested case control (NCC) study is a variation of a case-control study in which only a subset of controls from the cohort are compared to the incident cases. In a case-cohort study, all incident cases in the cohort are compared to a random subset of participants who do not develop the disease of interest.Protein primary structure: The primary structure of a peptide or protein is the linear sequence of its amino acid structural units, and partly comprises its overall biomolecular structure. By convention, the primary structure of a protein is reported starting from the amino-terminal (N) end to the carboxyl-terminal (C) end.Silent mutation: Silent mutations are mutations in DNA that do not significantly alter the phenotype of the organism in which they occur. Silent mutations can occur in non-coding regions (outside of genes or within introns), or they may occur within exons.Multiple Loci VNTR Analysis: Multiple Loci VNTR Analysis (MLVA ) is a method employed for the genetic analysis of particular microorganisms, such as pathogenic bacteria, that takes advantage of the polymorphism of tandemly repeated DNA sequences. A "VNTR" is a "variable-number tandem repeat".Mycobacterium tuberculosis complex: Mycobacterium tuberculosis complex refers to a genetically related group of Mycobacterium species that can cause tuberculosis in humans or other organisms.Pulsenet: PulseNet is a network run by the Centers for Disease Control and Prevention (CDC) which brings together public health and food regulatory agency laboratories around the United States.http://www.Exogenous bacteria: Exogenous bacteria are microorganisms introduced to closed biological systems from the external world. They exist in aquatic and terrestrial environments, as well as the atmosphere.CALERIE: CALERIE (Comprehensive Assessment of Long-term Effects of Reducing Intake of Energy) is a trial currently underway in the U.S.Direct repeat: Direct repeats are a type of genetic sequence that consists of two or more repeats of a specific sequence.Triparental mating: Triparental mating is a form of Bacterial conjugation where a conjugative plasmid present in one bacterial strain assists the transfer of a mobilizable plasmid present in a second bacterial strain into a third bacterial strain. Plasmids are introduced into bacteria for such purposes as transformation, cloning, or transposon mutagenesis.Phenotype microarray: The phenotype microarray approach is a technology for high-throughput phenotyping of cells.Pedigree chart: A pedigree chart is a diagram that shows the occurrence and appearance or phenotypes of a particular gene or organism and its ancestors from one generation to the next,pedigree chart Genealogy Glossary - About.com, a part of The New York Times Company.Gemmatimonadetes: The Gemmatimonadetes are a family of bacteria, given their own phylum (Gemmatimonadetes). This bacterium makes up about 2% of soil bacterial communities and has been identified as one of the top nine phyla found in soils; yet, there are currently only six cultured isolates.Single-strand conformation polymorphism: Single-strand conformation polymorphism (SSCP), or single-strand chain polymorphism, is defined as conformational difference of single-stranded nucleotide sequences of identical length as induced by differences in the sequences under certain experimental conditions. This property allows sequences to be distinguished by means of gel electrophoresis, which separates fragments according to their different conformations.Layout of the Port of Tianjin: The Port of Tianjin is divided into nine areas: the three core (“Tianjin Xingang”) areas of Beijiang, Nanjiang, and Dongjiang around the Xingang fairway; the Haihe area along the river; the Beitang port area around the Beitangkou estuary; the Dagukou port area in the estuary of the Haihe River; and three areas under construction (Hanggu, Gaoshaling, Nangang).Transfer-messenger RNA: Transfer-messenger RNA (abbreviated tmRNA, also known as 10Sa RNA and by its genetic name SsrA) is a bacterial RNA molecule with dual tRNA-like and messenger RNA-like properties. The tmRNA forms a ribonucleoprotein complex (tmRNP) together with Small Protein B (SmpB), Elongation Factor Tu (EF-Tu), and ribosomal protein S1.MT-RNR2: Mitochondrially encoded 16S RNA (often abbreviated as 16S) is a mitochondrial ribosomal RNA (rRNA) that in humans is encoded by the MT-RNR2 gene. The MT-RNR2 gene also encodes the Humanin polypeptide that has been the target of Alzheimer's disease research.Isocaudomer: Isocaudomers are pairs of restriction enzymes that have slightly different recognition sequences but upon cleavage generate identical termini. For example the enzymes Mbo I and BamH I are isocaudomers:RAPD: RAPD (pronounced "rapid") stands for 'Random Amplified Polymorphic DNA'. It is a type of PCR reaction, but the segments of DNA that are amplified are random.Alternative splicing: Alternative splicing is a regulated process during gene expression that results in a single gene coding for multiple proteins. In this process, particular exons of a gene may be included within or excluded from the final, processed messenger RNA (mRNA) produced from that gene.Niigata UniversityEcosystemList of strains of Escherichia coli: Escherichia coli is a well studied bacterium that was first identified by Theodor Escherich, after whom it was later named.Ferric uptake regulator family: In molecular biology, the ferric uptake regulator (FUR) family of proteins includes metal ion uptake regulator proteins. These are responsible for controlling the intracellular concentration of iron in many bacteria.Disequilibrium (medicine): Disequilibrium}}Tuberculosis managementRecombination (cosmology): In cosmology, recombination refers to the epoch at which charged electrons and protons first became bound to form electrically neutral hydrogen atoms.Note that the term recombination is a misnomer, considering that it represents the first time that electrically neutral hydrogen formed.National Outbreak Reporting System: ==The National Outbreak Reporting System (NORS)==Haplogroup L0 (mtDNA)Codon Adaptation Index: The Codon Adaptation Index (CAI) is the most widespread technique for analyzing Codon usage bias. As opposed to other measures of codon usage bias, such as the 'effective number of codons' (Nc), which measure deviation from a uniform bias (null hypothesis), CAI measures the deviation of a given protein coding gene sequence with respect to a reference set of genes.Beef cattle: Beef cattle are cattle raised for meat production (as distinguished from dairy cattle, used for milk production). The meat of adult cattle is known as beef.QRISK: QRISK2 (the most recent version of QRISK) is a prediction algorithm for cardiovascular disease (CVD) that uses traditional risk factors (age, systolic blood pressure, smoking status and ratio of total serum cholesterol to high-density lipoprotein cholesterol) together with body mass index, ethnicity, measures of deprivation, family history, chronic kidney disease, rheumatoid arthritis, atrial fibrillation, diabetes mellitus, and antihypertensive treatment.Smith–Fineman–Myers syndrome: Smith–Fineman–Myers syndrome (SFMS1), also called X-linked mental retardation-hypotonic facies syndrome 1 (MRXHF1), Carpenter–Waziri syndrome, Chudley–Lowry syndrome, SFMS, Holmes–Gang syndrome and Juberg–Marsidi syndrome (JMS), is a rare X-linked recessive congenital disorder that causes birth defects. This syndrome was named after 3 men, Richard D.GC box: In molecular biology, a GC box is a distinct pattern of nucleotides found in the promoter region of some eukaryotic genes upstream of the TATA box and approximately 110 bases upstream from the transcription initiation site. It has a consensus sequence GGGCGG which is position dependent and orientation independent.Point mutationParaHox: The ParaHox gene cluster is an array of homeobox genes (involved in morphogenesis, the regulation of patterns of anatomical development) from the Gsx, Xlox (Pdx) and Cdx gene families.Methylenetetrahydrofolate reductase: Methylene tetrahydrofolate reductase (MTHFR) is the rate-limiting enzyme in the methyl cycle, and it is encoded by the MTHFR gene. Methylenetetrahydrofolate reductase catalyzes the conversion of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate, a cosubstrate for homocysteine remethylation to methionine.

(1/8395) Identification of DNA polymorphisms associated with the V type alpha1-antitrypsin gene.

alpha1-Antitrypsin (alpha1-AT) is a highly polymorphic protein. The V allele of alpha1-AT has been shown to be associated with focal glomerulosclerosis (FGS) in Negroid and mixed race South African patients. To identify mutations and polymorphisms in the gene for the V allele of alpha1-AT in five South African patients with FGS nephrotic syndrome DNA sequence analysis and restriction fragment length polymorphisms of the coding exons were carried out. Four of the patients were heterozygous for the BstEII RFLP in exon III [M1(Val213)(Ala213)] and one patient was a M1(Ala213) homozygote. The mutation for the V allele was identified in exon II as Gly-148 (GGG)-->Arg (AGG) and in all patients was associated with a silent mutation at position 158 (AAC-->AAT). The patient who was homozygous for (Ala213) also had a silent mutation at position 256 in exon III (GAT-->GAC) which was not present in any of the other four patients. Although the V allele of alpha1-AT is not associated with severe plasma deficiency, it may be in linkage disequilibrium with other genes on chromosome 14 that predispose to FGS. Furthermore, the associated silent mutation at position 158 and the Ala213 polymorphism are of interest, as these could represent an evolutionary intermediate between the M1(Ala213) and M1(Val213) subtypes.  (+info)

(2/8395) Identification of a cytolethal distending toxin gene locus and features of a virulence-associated region in Actinobacillus actinomycetemcomitans.

A genetic locus for a cytolethal distending toxin (CDT) was identified in a polymorphic region of the chromosome of Actinobacillus actinomycetemcomitans, a predominant oral pathogen. The locus was comprised of three open reading frames (ORFs) that had significant amino acid sequence similarity and more than 90% sequence identity to the cdtABC genes of some pathogenic Escherichia coli strains and Haemophilus ducreyi, respectively. Sonic extracts from recombinant E. coli, containing the A. actinomycetemcomitans ORFs, caused the distension and killing of Chinese hamster ovary cells characteristic of a CDT. Monoclonal antibodies made reactive with the CdtA, CdtB, and CdtC proteins of H. ducreyi recognized the corresponding gene products from the recombinant strain. CDT-like activities were no longer expressed by the recombinant strain when an OmegaKan-2 interposon was inserted into the cdtA and cdtB genes. Expression of the CDT-like activities in A. actinomycetemcomitans was strain specific. Naturally occurring expression-negative strains had large deletions within the region of the cdt locus. The cdtABC genes were flanked by an ORF (virulence plasmid protein), a partial ORF (integrase), and DNA sequences (bacteriophage integration site) characteristic of virulence-associated regions. These results provide evidence for a functional CDT in a human oral pathogen.  (+info)

(3/8395) Role of the angiotensin type 2 receptor gene in congenital anomalies of the kidney and urinary tract, CAKUT, of mice and men.

Angiotensin type 2 receptor gene null mutant mice display congenital anomalies of the kidney and urinary tract (CAKUT). Various features of mouse CAKUT impressively mimic human CAKUT. Studies of the human type 2 receptor (AGTR2) gene in two independent cohorts found that a significant association exists between CAKUT and a nucleotide transition within the lariat branchpoint motif of intron 1, which perturbs AGTR2 mRNA splicing efficiency. AGTR2, therefore, has a significant ontogenic role for the kidney and urinary tract system. Studies revealed that the establishment of CAKUT is preceded by delayed apoptosis of undifferentiated mesenchymal cells surrounding the urinary tract during key ontogenic events, from the ureteral budding to the expansive growth of the kidney and ureter.  (+info)

(4/8395) Diversity of rhizobia associated with Amorpha fruticosa isolated from Chinese soils and description of Mesorhizobium amorphae sp. nov.

Fifty-five Chinese isolates from nodules of Amorpha fruticosa were characterized and compared with the type strains of the species and genera of bacteria which form nitrogen-fixing symbioses with leguminous host plants. A polyphasic approach, which included RFLP of PCR-amplified 16S rRNA genes, multilocus enzyme electrophoresis (MLEE), DNA-DNA hybridization, 16S rRNA gene sequencing, electrophoretic plasmid profiles, cross-nodulation and a phenotypic study, was used in the comparative analysis. The isolates originated from several different sites in China and they varied in their phenotypic and genetic characteristics. The majority of the isolates had moderate to slow growth rates, produced acid on YMA and harboured a 930 kb symbiotic plasmid (pSym). Five different RFLP patterns were identified among the 16S rRNA genes of all the isolates. Isolates grouped by PCR-RFLP of the 16S rRNA genes were also separated into groups by variation in MLEE profiles and by DNA-DNA hybridization. A representative isolate from each of these DNA homology groups had a separate position in a phylogenetic tree as determined from sequencing analysis of the 16S rRNA genes. A new species, Mesorhizobium amorphae, is proposed for the majority of the isolates, which belonged to a moderately slow- to slow-growing, acid-producing group based upon their distinct phylogenetic position, their unique electrophoretic type, their low DNA homology with reference strains representing the species within the genus Mesorhizobium and their distinct phenotypic features. Strain ACCC 19665 was chosen as the type strain for M. amorphae sp. nov.  (+info)

(5/8395) RFLP of rRNA genes and sequencing of the 16S-23S rDNA intergenic spacer region of ammonia-oxidizing bacteria: a phylogenetic approach.

It has been established that 16S rRNA gene-based phylogeny gives a low resolution between members of the chemoautotrophic ammonia-oxidizing bacteria (AOB) belonging to the beta-subclass of the Proteobacteria. In this study, 12 isolates of AOB were ribotyped, and the sequences of the 16S-23S rDNA intergenic spacer region (ISR) were determined and used in a phylogenetic study. 16S and 23S rDNA ribotyping revealed that the AOB studied contain only one rrn operon per genome, in contrast to most bacteria, which have 5-10 copies of the rRNA genes per genome. It is likely that the presence of only one set of rRNA genes is related to the slow growth of the AOB. The 16S and 23S rRNA genes of the AOB were shown to be arranged in the classical way: a 16S rRNA gene, an ISR and a 23S rRNA gene. Despite the close phylogenetic relationship among the AOB, the relative location of the rRNA genes in the genome appears to vary considerably. The size of the ISR was approximately 400 bp in the Nitrosomonas isolates and 645-694 bp in the Nitrosospira isolates, suggesting a species-specific size difference in the ISR. The ISR contained two potential tRNA genes in the 5' end in all isolates studied. The similarity values between the ISR sequences of the AOB are low (42.9-96.2%) compared with the 16S rDNA sequence similarity values, and therefore the ISR sequences are valuable as a complementary phylogenetic tool in combination with 16S rRNA gene sequences. The phylogenetic analysis of the AOB based on ISR sequences confirms the 16S rRNA gene-based phylogeny but has the benefit of giving a higher resolution.  (+info)

(6/8395) Identification of yeasts by RFLP analysis of the 5.8S rRNA gene and the two ribosomal internal transcribed spacers.

The identification and classification of yeasts have traditionally been based on morphological, physiological and biochemical traits. Various kits have been developed as rapid systems for yeast identification, but mostly for clinical diagnosis. In recent years, different molecular biology techniques have been developed for yeast identification, but there is no available database to identify a large number of species. In the present study, the restriction patterns generated from the region spanning the internal transcribed spacers (ITS1 and ITS2) and the 5.8S rRNA gene were used to identify a total of 132 yeast species belonging to 25 different genera, including teleomorphic and anamorphic ascomycetous and basidiomycetous yeasts. In many cases, the size of the PCR products and the restriction patterns obtained with endonucleases CfoI, HaeIII and HinfI yielded a unique profile for each species. Accordingly, the use of this molecular approach is proposed as a new rapid and easy method of routine yeast identification.  (+info)

(7/8395) Epidemiological characterization of methicillin-resistant Staphylococcus aureus isolated in the North West of England by protein A (spa) and coagulase (coa) gene polymorphisms.

In a comparative study, isolates of methicillin-resistant Staphylococcus aureus (MRSA) with known pulsed-field gel electrophoresis (PFGE) and bacteriophage type were analysed by polymerase chain reaction (PCR) and restriction fragment length polymorphisms (RFLP) for additional discriminatory subtyping information. PFGE was previously performed using standardized, commercially available kits and pre-programmed software. Isolates were examined for coagulase (coa) and protein A (spa) gene polymorphisms following PCR amplification of the coa hypervariable and spa repeat regions. Coa gene RFLPs produced a total of 38 distinct combined patterns after digestion with HaeIII and AluI and identified the predominant epidemic (EMRSA) types 15 and 16. A unique HaeIII restriction site was identified by RFLP and sequence analysis in the coa gene for EMRSA 15 but not EMRSA 16. The spa gene PCR yielded a total of 14 different profiles ranging from 3-18 repeats with the 2 predominant EMRSA types falling into 2 distinct groups. PCR detection of coa and spa polymorphisms offer a rapid preliminary strain identification and discriminatory subtyping information for surveillance of MRSA.  (+info)

(8/8395) Molecular cloning and characterization of three cDNAs encoding putative mitogen-activated protein kinase kinases (MAPKKs) in Arabidopsis thaliana.

We isolated three Arabidopsis thaliana cDNA clones (ATMKK3, ATMKK4 and ATMKK5) encoding protein kinases with extensive homology to the mitogen-activated protein kinase kinases (MAPKKs) of various organisms in the catalytic domain. ATMKK3 shows high homology (85% identity) to NPK2, a tobacco MAPKK homologue. ATMKK4 and 5 are closely related to each other (84% identity). Phylogenetic analysis showed that the plant MAPKKs constitute at least three subgroups. The recombinant ATMKK3 and ATMKK4 were expressed as a fusion protein with glutathione S-transferase (GST) in Escherichia coli. Affinity purified GST-ATMKK3 and GST-ATMKK4 proteins contained phosphorylation activity, which shows that both the ATMKK3 and ATMKK4 genes encode functional protein kinases. Northern blot analysis revealed that the ATMKK3 gene expressed in all the organs. The levels of ATMKK4 and 5 mRNAs were relatively higher in steins and leaves than in flowers and roots. We determined the map positions of the ATMKK3, 4 and 5 genes on Arabidopsis chromosomes by RFLP mapping using P1 genomic clones.  (+info)


How much length will i loose off my hair from getting a louse perm?


I'm planning to get a lose perm done, but i want to keep the length off my hair, so want to grow extra length i would lose from having a loose perm done? My hair is half way down my back and want to keep it that length since it took 6 years and one pregnancy to get it this long.

Can anyone tell me who's had a perm of any kind, tell me how much length they lost off their hair?
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I've had my perm for a year and a half now. Before I got my perm my hair was half way down my back and after the perm my hair was to my shoulders for the first couple of months because the curls were so tight and hadn't had time to relax. but after a while my new growth grew out a bit and my perm relaxed so my hair is still very very curly and back to its regular length and it did not take very long ... you've just got to give it some time :)


What length is used in a fresh nipple piercing and what length do I change after?


I'm planning on getting my nipple pierced, I want to know the length that is typically used and what length is changed after swelling. What size do piercers usually use, 14g or 16g?
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Length depends on your anatomy, that's something you'd want to ask a professional piercer about.
16g is a little too small, 14g is best in nipples.


What should be the ideal length that one should keep for a pair of jeans and trousers?


My length from my waist to the ground with my sandals or sneakers put on is 38.5 inches, so how many inches more should i add to the length of my jeans and trouser respectively to get the ideal fall?
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38.5 inch is enough.


What length should I get my hair cut to?


My hair is at the middle of my back in length right now, and when it falls in front it makes my face look really small and weird, plus I have bangs. I'm trying to decide if I want to get rid of my bangs and go a little shorter or just get it shorter. 

I don't want to look like a little girl or something, that's why I've kept it long. Maybe I could try to thin my bangs out..lol..

Anyways, any tips/suggestions?
Oh, and my bangs are longer than in this avatar, they're eyebrow length exactly.
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Dont cut your hair, I have thick long dark brown hair and side bangs and get compliments all the time, just part your hair on the side and side swipe them! try a head and too, long hair is very flattering on a girl!


What can I do to thicken my hair without cutting off the length where it is starting to thin?


My hair has length, but it is thin and broken off around the face and  in the back.  I don't want to cut it all off and start all over.  So what can I do to treat it to make it thincken up and become more healthy without losing my length.
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Strengthen it with a good protien conditioner. Use volumizing products.  Get a good haircut that won't remove length, but get rid of a lot of the dead ends.  You'd be surprised at the difference an inch or less off can make.  Or you can always try extensions!!!

check Traceyb out at lovelyextensions.com


What is the optimum length natural hair should be before getting clip in hair extensions?


My hair is about 3 inches at it's shortest length.
I would like to get hair extensions to grow my hair out with, but I'm not sure if my hair's long enough yet?
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You can put hair extensions in at any length but it can sometimes look really obvious, the best length would have to be about a inch shorter then your shoulder for a more realistic look.


How can I tell when I ovulate if my cycle length is different each month?


My cycle changes length almost every month; not dramatically, just a few days, but it does change. I the past 3 months since I started keeping track  I've had a 28 day, 32 day, and 26 day cycle. I never know when I'm going to ovulate because I never know how long this cycle will be.
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Check for eggwhite cervical fluid.  It usually appears a few days before ovulation, lasts for a few days, and then disappears after ovulation.  This is because it serves a very important purpose:  Helping sperm swim easier and survive longer, PRECISELY when your body wants them to.  Isn't Mother Nature beautiful?

So either look on your toilet tissue regularly or, to be more accurate, swipe a clean finger through your vagina once a day.  There are several types of healthy, normal cervical fluid ("vaginal discharge") that you may find throughout your cycle-- don't get worried that the changing types means anything negative.  

1)  Shortly after your period ends, you may find "sticky" that may resemble half-dried paste with chunks and everything.  :)  It's considered infertile but perfectly normal.  

2)  You may find "creamy" which strongly resembles hand-lotion.  It indicates increasing fertility-- start watching more closely in the coming days or week.  

3)  Then you may find "egg-white," which looks just like what you would expect by the name:  usually clear, it's slippery, slimy, and stretchy.  If your body makes a lot of it, you may actually feel it just as you walk around during the day.  Fun, right?  Haha!  When you see egg-white fluid, take it as a strong sign that you may ovulate in the near future.  If you're trying to conceive, it means you should go get busy every single day you find this fluid!

4)  Then, the egg-white will usually disappear right after ovulation, returning you either to sticky-type fluid or no noticeable fluid at all.


P.S.  If you add temperature-charting to this (taking your temperature every morning at the same time before you get out of bed), you'll be practicing the most complete and easiest method to get to know your body that exists for cheap at-home use!  I've been doing it for years and always know when ovulate even when my cycle length changes.  Your temperature can verify that ovulation really did happen.  See my charts below for an example of how this information all comes together.

Good luck!


How can i increase the length of penis through natural exercises?


My penis length is only 5inch. Tell me the natural way to increase the length of it. Without using any exercise machine or medicine.
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5 inch is pleanty,and there are no ways to increase its size until a decade in the future by some wonder drug