Polymerase Chain Reaction: In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.Reverse Transcriptase Polymerase Chain Reaction: A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.DNA-Directed DNA Polymerase: DNA-dependent DNA polymerases found in bacteria, animal and plant cells. During the replication process, these enzymes catalyze the addition of deoxyribonucleotide residues to the end of a DNA strand in the presence of DNA as template-primer. They also possess exonuclease activity and therefore function in DNA repair.RNA, Messenger: RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.DNA Primers: Short sequences (generally about 10 base pairs) of DNA that are complementary to sequences of messenger RNA and allow reverse transcriptases to start copying the adjacent sequences of mRNA. Primers are used extensively in genetic and molecular biology techniques.Real-Time Polymerase Chain Reaction: Methods used for detecting the amplified DNA products from the polymerase chain reaction as they accumulate instead of at the end of the reaction.RNA Polymerase II: A DNA-dependent RNA polymerase present in bacterial, plant, and animal cells. It functions in the nucleoplasmic structure and transcribes DNA into RNA. It has different requirements for cations and salt than RNA polymerase I and is strongly inhibited by alpha-amanitin. EC 2.7.7.6.Sensitivity and Specificity: Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed)DNA, Viral: Deoxyribonucleic acid that makes up the genetic material of viruses.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.DNA, Bacterial: Deoxyribonucleic acid that makes up the genetic material of bacteria.DNA: A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).Cloning, Molecular: The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.DNA-Directed RNA Polymerases: Enzymes that catalyze DNA template-directed extension of the 3'-end of an RNA strand one nucleotide at a time. They can initiate a chain de novo. In eukaryotes, three forms of the enzyme have been distinguished on the basis of sensitivity to alpha-amanitin, and the type of RNA synthesized. (From Enzyme Nomenclature, 1992).Transcription, Genetic: The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.Genotype: The genetic constitution of the individual, comprising the ALLELES present at each GENETIC LOCUS.DNA Polymerase I: A DNA-dependent DNA polymerase characterized in prokaryotes and may be present in higher organisms. It has both 3'-5' and 5'-3' exonuclease activity, but cannot use native double-stranded DNA as template-primer. It is not inhibited by sulfhydryl reagents and is active in both DNA synthesis and repair. EC 2.7.7.7.Blotting, Southern: A method (first developed by E.M. Southern) for detection of DNA that has been electrophoretically separated and immobilized by blotting on nitrocellulose or other type of paper or nylon membrane followed by hybridization with labeled NUCLEIC ACID PROBES.Oligonucleotide Probes: Synthetic or natural oligonucleotides used in hybridization studies in order to identify and study specific nucleic acid fragments, e.g., DNA segments near or within a specific gene locus or gene. The probe hybridizes with a specific mRNA, if present. Conventional techniques used for testing for the hybridization product include dot blot assays, Southern blot assays, and DNA:RNA hybrid-specific antibody tests. Conventional labels for the probe include the radioisotope labels 32P and 125I and the chemical label biotin.Mutation: Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.Gene Expression: The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.DNA Polymerase II: A DNA-dependent DNA polymerase characterized in E. coli and other lower organisms. It may be present in higher organisms and has an intrinsic molecular activity only 5% of that of DNA Polymerase I. This polymerase has 3'-5' exonuclease activity, is effective only on duplex DNA with gaps or single-strand ends of less than 100 nucleotides as template, and is inhibited by sulfhydryl reagents. EC 2.7.7.7.Multiplex Polymerase Chain Reaction: Methods for using more than one primer set in a polymerase chain reaction to amplify more than one segment of the target DNA sequence in a single reaction.Polymorphism, Restriction Fragment Length: Variation occurring within a species in the presence or length of DNA fragment generated by a specific endonuclease at a specific site in the genome. Such variations are generated by mutations that create or abolish recognition sites for these enzymes or change the length of the fragment.DNA Polymerase III: A DNA-dependent DNA polymerase characterized in E. coli and other lower organisms but may be present in higher organisms. Use also for a more complex form of DNA polymerase III designated as DNA polymerase III* or pol III* which is 15 times more active biologically than DNA polymerase I in the synthesis of DNA. This polymerase has both 3'-5' and 5'-3' exonuclease activities, is inhibited by sulfhydryl reagents, and has the same template-primer dependence as pol II. EC 2.7.7.7.Sequence Analysis, DNA: A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.DNA, Protozoan: Deoxyribonucleic acid that makes up the genetic material of protozoa.Electrophoresis, Agar Gel: Electrophoresis in which agar or agarose gel is used as the diffusion medium.Gene Amplification: A selective increase in the number of copies of a gene coding for a specific protein without a proportional increase in other genes. It occurs naturally via the excision of a copy of the repeating sequence from the chromosome and its extrachromosomal replication in a plasmid, or via the production of an RNA transcript of the entire repeating sequence of ribosomal RNA followed by the reverse transcription of the molecule to produce an additional copy of the original DNA sequence. Laboratory techniques have been introduced for inducing disproportional replication by unequal crossing over, uptake of DNA from lysed cells, or generation of extrachromosomal sequences from rolling circle replication.DNA, Complementary: Single-stranded complementary DNA synthesized from an RNA template by the action of RNA-dependent DNA polymerase. cDNA (i.e., complementary DNA, not circular DNA, not C-DNA) is used in a variety of molecular cloning experiments as well as serving as a specific hybridization probe.Polymorphism, Genetic: The regular and simultaneous occurrence in a single interbreeding population of two or more discontinuous genotypes. The concept includes differences in genotypes ranging in size from a single nucleotide site (POLYMORPHISM, SINGLE NUCLEOTIDE) to large nucleotide sequences visible at a chromosomal level.DNA Probes: Species- or subspecies-specific DNA (including COMPLEMENTARY DNA; conserved genes, whole chromosomes, or whole genomes) used in hybridization studies in order to identify microorganisms, to measure DNA-DNA homologies, to group subspecies, etc. The DNA probe hybridizes with a specific mRNA, if present. Conventional techniques used for testing for the hybridization product include dot blot assays, Southern blot assays, and DNA:RNA hybrid-specific antibody tests. Conventional labels for the DNA probe include the radioisotope labels 32P and 125I and the chemical label biotin. The use of DNA probes provides a specific, sensitive, rapid, and inexpensive replacement for cell culture techniques for diagnosing infections.Alleles: Variant forms of the same gene, occupying the same locus on homologous CHROMOSOMES, and governing the variants in production of the same gene product.RNA, Viral: Ribonucleic acid that makes up the genetic material of viruses.Templates, Genetic: Macromolecular molds for the synthesis of complementary macromolecules, as in DNA REPLICATION; GENETIC TRANSCRIPTION of DNA to RNA, and GENETIC TRANSLATION of RNA into POLYPEPTIDES.RNA Polymerase I: A DNA-dependent RNA polymerase present in bacterial, plant, and animal cells. The enzyme functions in the nucleolar structure and transcribes DNA into RNA. It has different requirements for cations and salts than RNA polymerase II and III and is not inhibited by alpha-amanitin. EC 2.7.7.6.RNA Polymerase III: A DNA-dependent RNA polymerase present in bacterial, plant, and animal cells. It functions in the nucleoplasmic structure where it transcribes DNA into RNA. It has specific requirements for cations and salt and has shown an intermediate sensitivity to alpha-amanitin in comparison to RNA polymerase I and II. EC 2.7.7.6.Immunohistochemistry: Histochemical localization of immunoreactive substances using labeled antibodies as reagents.Immunoglobulin Heavy Chains: The largest of polypeptide chains comprising immunoglobulins. They contain 450 to 600 amino acid residues per chain, and have molecular weights of 51-72 kDa.Escherichia coli: A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.RNA-Directed DNA Polymerase: An enzyme that synthesizes DNA on an RNA template. It is encoded by the pol gene of retroviruses and by certain retrovirus-like elements. EC 2.7.7.49.DNA Polymerase beta: A DNA repair enzyme that catalyzes DNA synthesis during base excision DNA repair. EC 2.7.7.7.Promoter Regions, Genetic: DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.Sequence Homology, Nucleic Acid: The sequential correspondence of nucleotides in one nucleic acid molecule with those of another nucleic acid molecule. Sequence homology is an indication of the genetic relatedness of different organisms and gene function.Cells, Cultured: Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.Exons: The parts of a transcript of a split GENE remaining after the INTRONS are removed. They are spliced together to become a MESSENGER RNA or other functional RNA.Restriction Mapping: Use of restriction endonucleases to analyze and generate a physical map of genomes, genes, or other segments of DNA.DNA, Neoplasm: DNA present in neoplastic tissue.Cell Line: Established cell cultures that have the potential to propagate indefinitely.Sequence Homology, Amino Acid: The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.DNA Mutational Analysis: Biochemical identification of mutational changes in a nucleotide sequence.Nucleic Acid Hybridization: Widely used technique which exploits the ability of complementary sequences in single-stranded DNAs or RNAs to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (Kendrew, Encyclopedia of Molecular Biology, 1994, p503)In Situ Hybridization: A technique that localizes specific nucleic acid sequences within intact chromosomes, eukaryotic cells, or bacterial cells through the use of specific nucleic acid-labeled probes.Gene Expression Regulation: Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.Phylogeny: The relationships of groups of organisms as reflected by their genetic makeup.RNA: A polynucleotide consisting essentially of chains with a repeating backbone of phosphate and ribose units to which nitrogenous bases are attached. RNA is unique among biological macromolecules in that it can encode genetic information, serve as an abundant structural component of cells, and also possesses catalytic activity. (Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)Sequence Alignment: The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.Cattle: Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.Species Specificity: The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.Gene Expression Profiling: The determination of the pattern of genes expressed at the level of GENETIC TRANSCRIPTION, under specific circumstances or in a specific cell.Case-Control Studies: Studies which start with the identification of persons with a disease of interest and a control (comparison, referent) group without the disease. The relationship of an attribute to the disease is examined by comparing diseased and non-diseased persons with regard to the frequency or levels of the attribute in each group.Gene Frequency: The proportion of one particular in the total of all ALLELES for one genetic locus in a breeding POPULATION.Blotting, Western: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.Time Factors: Elements of limited time intervals, contributing to particular results or situations.Point Mutation: A mutation caused by the substitution of one nucleotide for another. This results in the DNA molecule having a change in a single base pair.Oligodeoxyribonucleotides: A group of deoxyribonucleotides (up to 12) in which the phosphate residues of each deoxyribonucleotide act as bridges in forming diester linkages between the deoxyribose moieties.Gene Rearrangement: The ordered rearrangement of gene regions by DNA recombination such as that which occurs normally during development.Evaluation Studies as Topic: Studies determining the effectiveness or value of processes, personnel, and equipment, or the material on conducting such studies. For drugs and devices, CLINICAL TRIALS AS TOPIC; DRUG EVALUATION; and DRUG EVALUATION, PRECLINICAL are available.Kinetics: The rate dynamics in chemical or physical systems.Immunoglobulin Light Chains: Polypeptide chains, consisting of 211 to 217 amino acid residues and having a molecular weight of approximately 22 kDa. There are two major types of light chains, kappa and lambda. Two Ig light chains and two Ig heavy chains (IMMUNOGLOBULIN HEAVY CHAINS) make one immunoglobulin molecule.Polymorphism, Single-Stranded Conformational: Variation in a population's DNA sequence that is detected by determining alterations in the conformation of denatured DNA fragments. Denatured DNA fragments are allowed to renature under conditions that prevent the formation of double-stranded DNA and allow secondary structure to form in single stranded fragments. These fragments are then run through polyacrylamide gels to detect variations in the secondary structure that is manifested as an alteration in migration through the gels.Nucleic Acid Amplification Techniques: Laboratory techniques that involve the in-vitro synthesis of many copies of DNA or RNA from one original template.Enzyme-Linked Immunosorbent Assay: An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.Paraffin Embedding: The infiltrating of tissue specimens with paraffin, as a supporting substance, to prepare for sectioning with a microtome.Feces: Excrement from the INTESTINES, containing unabsorbed solids, waste products, secretions, and BACTERIA of the DIGESTIVE SYSTEM.Blotting, Northern: Detection of RNA that has been electrophoretically separated and immobilized by blotting on nitrocellulose or other type of paper or nylon membrane followed by hybridization with labeled NUCLEIC ACID PROBES.Recombinant Proteins: Proteins prepared by recombinant DNA technology.Transcription Factors: Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process.Phenotype: The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment.Tumor Virus Infections: Infections produced by oncogenic viruses. The infections caused by DNA viruses are less numerous but more diverse than those caused by the RNA oncogenic viruses.RNA, Neoplasm: RNA present in neoplastic tissue.Taq Polymerase: A heat stable DNA-DIRECTED DNA POLYMERASE from the bacteria Thermus aquaticus. It is widely used for the amplification of genes through the process of POLYMERASE CHAIN REACTION. EC 2.7.7.-.DNA Replication: The process by which a DNA molecule is duplicated.Translocation, Genetic: A type of chromosome aberration characterized by CHROMOSOME BREAKAGE and transfer of the broken-off portion to another location, often to a different chromosome.RNA Replicase: An enzyme that catalyses RNA-template-directed extension of the 3'- end of an RNA strand by one nucleotide at a time, and can initiate a chain de novo. (Enzyme Nomenclature, 1992, p293)Papillomaviridae: A family of small, non-enveloped DNA viruses infecting birds and most mammals, especially humans. They are grouped into multiple genera, but the viruses are highly host-species specific and tissue-restricted. They are commonly divided into hundreds of papillomavirus "types", each with specific gene function and gene control regions, despite sequence homology. Human papillomaviruses are found in the genera ALPHAPAPILLOMAVIRUS; BETAPAPILLOMAVIRUS; GAMMAPAPILLOMAVIRUS; and MUPAPILLOMAVIRUS.Dog Diseases: Diseases of the domestic dog (Canis familiaris). This term does not include diseases of wild dogs, WOLVES; FOXES; and other Canidae for which the heading CARNIVORA is used.Genetic Variation: Genotypic differences observed among individuals in a population.Reproducibility of Results: The statistical reproducibility of measurements (often in a clinical context), including the testing of instrumentation or techniques to obtain reproducible results. The concept includes reproducibility of physiological measurements, which may be used to develop rules to assess probability or prognosis, or response to a stimulus; reproducibility of occurrence of a condition; and reproducibility of experimental results.Oligonucleotide Array Sequence Analysis: Hybridization of a nucleic acid sample to a very large set of OLIGONUCLEOTIDE PROBES, which have been attached individually in columns and rows to a solid support, to determine a BASE SEQUENCE, or to detect variations in a gene sequence, GENE EXPRESSION, or for GENE MAPPING.DNA-Binding Proteins: Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases.Genes, Bacterial: The functional hereditary units of BACTERIA.Biopsy: Removal and pathologic examination of specimens in the form of small pieces of tissue from the living body.Electrophoresis, Polyacrylamide Gel: Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.Binding Sites: The parts of a macromolecule that directly participate in its specific combination with another molecule.Tumor Cells, Cultured: Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.Bacterial Proteins: Proteins found in any species of bacterium.DNA, Fungal: Deoxyribonucleic acid that makes up the genetic material of fungi.Up-Regulation: A positive regulatory effect on physiological processes at the molecular, cellular, or systemic level. At the molecular level, the major regulatory sites include membrane receptors, genes (GENE EXPRESSION REGULATION), mRNAs (RNA, MESSENGER), and proteins.Molecular Diagnostic Techniques: MOLECULAR BIOLOGY techniques used in the diagnosis of disease.Oligonucleotides: Polymers made up of a few (2-20) nucleotides. In molecular genetics, they refer to a short sequence synthesized to match a region where a mutation is known to occur, and then used as a probe (OLIGONUCLEOTIDE PROBES). (Dorland, 28th ed)Plasmids: Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.Genetic Predisposition to Disease: A latent susceptibility to disease at the genetic level, which may be activated under certain conditions.Genetic Markers: A phenotypically recognizable genetic trait which can be used to identify a genetic locus, a linkage group, or a recombination event.Poly(ADP-ribose) Polymerases: Enzymes that catalyze the transfer of multiple ADP-RIBOSE groups from nicotinamide-adenine dinucleotide (NAD) onto protein targets, thus building up a linear or branched homopolymer of repeating ADP-ribose units i.e., POLY ADENOSINE DIPHOSPHATE RIBOSE.Viral Proteins: Proteins found in any species of virus.Protein Binding: The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.Cattle Diseases: Diseases of domestic cattle of the genus Bos. It includes diseases of cows, yaks, and zebus.Neoplasm Proteins: Proteins whose abnormal expression (gain or loss) are associated with the development, growth, or progression of NEOPLASMS. Some neoplasm proteins are tumor antigens (ANTIGENS, NEOPLASM), i.e. they induce an immune reaction to their tumor. Many neoplasm proteins have been characterized and are used as tumor markers (BIOMARKERS, TUMOR) when they are detectable in cells and body fluids as monitors for the presence or growth of tumors. Abnormal expression of ONCOGENE PROTEINS is involved in neoplastic transformation, whereas the loss of expression of TUMOR SUPPRESSOR PROTEINS is involved with the loss of growth control and progression of the neoplasm.Gene Expression Regulation, Neoplastic: Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in neoplastic tissue.Gene Rearrangement, B-Lymphocyte, Heavy Chain: Ordered rearrangement of B-lymphocyte variable gene regions of the IMMUNOGLOBULIN HEAVY CHAINS, thereby contributing to antibody diversity. It occurs during the first stage of differentiation of the IMMATURE B-LYMPHOCYTES.Swine: Any of various animals that constitute the family Suidae and comprise stout-bodied, short-legged omnivorous mammals with thick skin, usually covered with coarse bristles, a rather long mobile snout, and small tail. Included are the genera Babyrousa, Phacochoerus (wart hogs), and Sus, the latter containing the domestic pig (see SUS SCROFA).DNA, Helminth: Deoxyribonucleic acid that makes up the genetic material of helminths.Herpesviridae Infections: Virus diseases caused by the HERPESVIRIDAE.Ligase Chain Reaction: A DNA amplification technique based upon the ligation of OLIGONUCLEOTIDE PROBES. The probes are designed to exactly match two adjacent sequences of a specific target DNA. The chain reaction is repeated in three steps in the presence of excess probe: (1) heat denaturation of double-stranded DNA, (2) annealing of probes to target DNA, and (3) joining of the probes by thermostable DNA ligase. After the reaction is repeated for 20-30 cycles the production of ligated probe is measured.Transfection: The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.Gene Library: A large collection of DNA fragments cloned (CLONING, MOLECULAR) from a given organism, tissue, organ, or cell type. It may contain complete genomic sequences (GENOMIC LIBRARY) or complementary DNA sequences, the latter being formed from messenger RNA and lacking intron sequences.Neoplasm, Residual: Remnant of a tumor or cancer after primary, potentially curative therapy. (Dr. Daniel Masys, written communication)Gene Deletion: A genetic rearrangement through loss of segments of DNA or RNA, bringing sequences which are normally separated into close proximity. This deletion may be detected using cytogenetic techniques and can also be inferred from the phenotype, indicating a deletion at one specific locus.Cell Line, Tumor: A cell line derived from cultured tumor cells.Pedigree: The record of descent or ancestry, particularly of a particular condition or trait, indicating individual family members, their relationships, and their status with respect to the trait or condition.Macromolecular Substances: Compounds and molecular complexes that consist of very large numbers of atoms and are generally over 500 kDa in size. In biological systems macromolecular substances usually can be visualized using ELECTRON MICROSCOPY and are distinguished from ORGANELLES by the lack of a membrane structure.China: A country spanning from central Asia to the Pacific Ocean.Pregnancy: The status during which female mammals carry their developing young (EMBRYOS or FETUSES) in utero before birth, beginning from FERTILIZATION to BIRTH.RNA, Ribosomal, 16S: Constituent of 30S subunit prokaryotic ribosomes containing 1600 nucleotides and 21 proteins. 16S rRNA is involved in initiation of polypeptide synthesis.Chromosomes, Human, Pair 14: A specific pair of GROUP D CHROMOSOMES of the human chromosome classification.Cytomegalovirus: A genus of the family HERPESVIRIDAE, subfamily BETAHERPESVIRINAE, infecting the salivary glands, liver, spleen, lungs, eyes, and other organs, in which they produce characteristically enlarged cells with intranuclear inclusions. Infection with Cytomegalovirus is also seen as an opportunistic infection in AIDS.DNA, Ribosomal: DNA sequences encoding RIBOSOMAL RNA and the segments of DNA separating the individual ribosomal RNA genes, referred to as RIBOSOMAL SPACER DNA.Flow Cytometry: Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.Genes, Immunoglobulin: Genes encoding the different subunits of the IMMUNOGLOBULINS, for example the IMMUNOGLOBULIN LIGHT CHAIN GENES and the IMMUNOGLOBULIN HEAVY CHAIN GENES. The heavy and light immunoglobulin genes are present as gene segments in the germline cells. The completed genes are created when the segments are shuffled and assembled (B-LYMPHOCYTE GENE REARRANGEMENT) during B-LYMPHOCYTE maturation. The gene segments of the human light and heavy chain germline genes are symbolized V (variable), J (joining) and C (constant). The heavy chain germline genes have an additional segment D (diversity).Heterozygote: An individual having different alleles at one or more loci regarding a specific character.Liver: A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.Prevalence: The total number of cases of a given disease in a specified population at a designated time. It is differentiated from INCIDENCE, which refers to the number of new cases in the population at a given time.Myosin Heavy Chains: The larger subunits of MYOSINS. The heavy chains have a molecular weight of about 230 kDa and each heavy chain is usually associated with a dissimilar pair of MYOSIN LIGHT CHAINS. The heavy chains possess actin-binding and ATPase activity.Genes: A category of nucleic acid sequences that function as units of heredity and which code for the basic instructions for the development, reproduction, and maintenance of organisms.Protozoan Infections, Animal: Infections with unicellular organisms formerly members of the subkingdom Protozoa. The infections may be experimental or veterinary.Introns: Sequences of DNA in the genes that are located between the EXONS. They are transcribed along with the exons but are removed from the primary gene transcript by RNA SPLICING to leave mature RNA. Some introns code for separate genes.Asian Continental Ancestry Group: Individuals whose ancestral origins are in the southeastern and eastern areas of the Asian continent.Swine Diseases: Diseases of domestic swine and of the wild boar of the genus Sus.Codon: A set of three nucleotides in a protein coding sequence that specifies individual amino acids or a termination signal (CODON, TERMINATOR). Most codons are universal, but some organisms do not produce the transfer RNAs (RNA, TRANSFER) complementary to all codons. These codons are referred to as unassigned codons (CODONS, NONSENSE).Immunoenzyme Techniques: Immunologic techniques based on the use of: (1) enzyme-antibody conjugates; (2) enzyme-antigen conjugates; (3) antienzyme antibody followed by its homologous enzyme; or (4) enzyme-antienzyme complexes. These are used histologically for visualizing or labeling tissue specimens.Chromosome Mapping: Any method used for determining the location of and relative distances between genes on a chromosome.Chromosomes, Human, Pair 18: A specific pair of GROUP E CHROMOSOMES of the human chromosome classification.Models, Molecular: Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures.Horse Diseases: Diseases of domestic and wild horses of the species Equus caballus.Tumor Markers, Biological: Molecular products metabolized and secreted by neoplastic tissue and characterized biochemically in cells or body fluids. They are indicators of tumor stage and grade as well as useful for monitoring responses to treatment and predicting recurrence. Many chemical groups are represented including hormones, antigens, amino and nucleic acids, enzymes, polyamines, and specific cell membrane proteins and lipids.Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor: Ordered rearrangement of T-cell variable gene regions coding for the gamma-chain of antigen receptors.DNA Fingerprinting: A technique for identifying individuals of a species that is based on the uniqueness of their DNA sequence. Uniqueness is determined by identifying which combination of allelic variations occur in the individual at a statistically relevant number of different loci. In forensic studies, RESTRICTION FRAGMENT LENGTH POLYMORPHISM of multiple, highly polymorphic VNTR LOCI or MICROSATELLITE REPEAT loci are analyzed. The number of loci used for the profile depends on the ALLELE FREQUENCY in the population.Molecular Weight: The sum of the weight of all the atoms in a molecule.Dogs: The domestic dog, Canis familiaris, comprising about 400 breeds, of the carnivore family CANIDAE. They are worldwide in distribution and live in association with people. (Walker's Mammals of the World, 5th ed, p1065)Carrier Proteins: Transport proteins that carry specific substances in the blood or across cell membranes.Disease Outbreaks: Sudden increase in the incidence of a disease. The concept includes EPIDEMICS and PANDEMICS.Herpesvirus 6, Human: The type species of ROSEOLOVIRUS isolated from patients with AIDS and other LYMPHOPROLIFERATIVE DISORDERS. It infects and replicates in fresh and established lines of hematopoietic cells and cells of neural origin. It also appears to alter NK cell activity. HHV-6; (HBLV) antibodies are elevated in patients with AIDS, Sjogren's syndrome, sarcoidosis, chronic fatigue syndrome, and certain malignancies. HHV-6 is the cause of EXANTHEMA SUBITUM and has been implicated in encephalitis.Tissue Fixation: The technique of using FIXATIVES in the preparation of cytologic, histologic, or pathologic specimens for the purpose of maintaining the existing form and structure of all the constituent elements.Parvoviridae Infections: Virus infections caused by the PARVOVIRIDAE.Leukocytes, Mononuclear: Mature LYMPHOCYTES and MONOCYTES transported by the blood to the body's extravascular space. They are morphologically distinguishable from mature granulocytic leukocytes by their large, non-lobed nuclei and lack of coarse, heavily stained cytoplasmic granules.Papillomavirus Infections: Neoplasms of the skin and mucous membranes caused by papillomaviruses. They are usually benign but some have a high risk for malignant progression.Herpesvirus 4, Human: The type species of LYMPHOCRYPTOVIRUS, subfamily GAMMAHERPESVIRINAE, infecting B-cells in humans. It is thought to be the causative agent of INFECTIOUS MONONUCLEOSIS and is strongly associated with oral hairy leukoplakia (LEUKOPLAKIA, HAIRY;), BURKITT LYMPHOMA; and other malignancies.Repetitive Sequences, Nucleic Acid: Sequences of DNA or RNA that occur in multiple copies. There are several types: INTERSPERSED REPETITIVE SEQUENCES are copies of transposable elements (DNA TRANSPOSABLE ELEMENTS or RETROELEMENTS) dispersed throughout the genome. TERMINAL REPEAT SEQUENCES flank both ends of another sequence, for example, the long terminal repeats (LTRs) on RETROVIRUSES. Variations may be direct repeats, those occurring in the same direction, or inverted repeats, those opposite to each other in direction. TANDEM REPEAT SEQUENCES are copies which lie adjacent to each other, direct or inverted (INVERTED REPEAT SEQUENCES).Fluorescent Antibody Technique, Direct: A form of fluorescent antibody technique utilizing a fluorochrome conjugated to an antibody, which is added directly to a tissue or cell suspension for the detection of a specific antigen. (Bennington, Saunders Dictionary & Encyclopedia of Laboratory Medicine and Technology, 1984)Rats, Sprague-Dawley: A strain of albino rat used widely for experimental purposes because of its calmness and ease of handling. It was developed by the Sprague-Dawley Animal Company.Infant, Newborn: An infant during the first month after birth.Cytomegalovirus Infections: Infection with CYTOMEGALOVIRUS, characterized by enlarged cells bearing intranuclear inclusions. Infection may be in almost any organ, but the salivary glands are the most common site in children, as are the lungs in adults.Polymorphism, Single Nucleotide: A single nucleotide variation in a genetic sequence that occurs at appreciable frequency in the population.Chlamydia Infections: Infections with bacteria of the genus CHLAMYDIA.Sequence Deletion: Deletion of sequences of nucleic acids from the genetic material of an individual.Disease Models, Animal: Naturally occurring or experimentally induced animal diseases with pathological processes sufficiently similar to those of human diseases. They are used as study models for human diseases.Clone Cells: A group of genetically identical cells all descended from a single common ancestral cell by mitosis in eukaryotes or by binary fission in prokaryotes. Clone cells also include populations of recombinant DNA molecules all carrying the same inserted sequence. (From King & Stansfield, Dictionary of Genetics, 4th ed)Membrane Proteins: Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors.Homozygote: An individual in which both alleles at a given locus are identical.HeLa Cells: The first continuously cultured human malignant CELL LINE, derived from the cervical carcinoma of Henrietta Lacks. These cells are used for VIRUS CULTIVATION and antitumor drug screening assays.Specimen Handling: Procedures for collecting, preserving, and transporting of specimens sufficiently stable to provide accurate and precise results suitable for clinical interpretation.Bone Marrow: The soft tissue filling the cavities of bones. Bone marrow exists in two types, yellow and red. Yellow marrow is found in the large cavities of large bones and consists mostly of fat cells and a few primitive blood cells. Red marrow is a hematopoietic tissue and is the site of production of erythrocytes and granular leukocytes. Bone marrow is made up of a framework of connective tissue containing branching fibers with the frame being filled with marrow cells.Aborted Fetus: A mammalian fetus expelled by INDUCED ABORTION or SPONTANEOUS ABORTION.RNA, Ribosomal, 18S: Constituent of the 40S subunit of eukaryotic ribosomes. 18S rRNA is involved in the initiation of polypeptide synthesis in eukaryotes.Herpesviridae: A family of enveloped, linear, double-stranded DNA viruses infecting a wide variety of animals. Subfamilies, based on biological characteristics, include: ALPHAHERPESVIRINAE; BETAHERPESVIRINAE; and GAMMAHERPESVIRINAE.DNA, Kinetoplast: DNA of kinetoplasts which are specialized MITOCHONDRIA of trypanosomes and related parasitic protozoa within the order KINETOPLASTIDA. Kinetoplast DNA consists of a complex network of numerous catenated rings of two classes; the first being a large number of small DNA duplex rings, called minicircles, approximately 2000 base pairs in length, and the second being several dozen much larger rings, called maxicircles, approximately 37 kb in length.Gene Expression Regulation, Enzymologic: Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in enzyme synthesis.In Situ Hybridization, Fluorescence: A type of IN SITU HYBRIDIZATION in which target sequences are stained with fluorescent dye so their location and size can be determined using fluorescence microscopy. This staining is sufficiently distinct that the hybridization signal can be seen both in metaphase spreads and in interphase nuclei.Prognosis: A prediction of the probable outcome of a disease based on a individual's condition and the usual course of the disease as seen in similar situations.Saccharomyces cerevisiae: A species of the genus SACCHAROMYCES, family Saccharomycetaceae, order Saccharomycetales, known as "baker's" or "brewer's" yeast. The dried form is used as a dietary supplement.BrazilFluorescent Antibody Technique, Indirect: A form of fluorescent antibody technique commonly used to detect serum antibodies and immune complexes in tissues and microorganisms in specimens from patients with infectious diseases. The technique involves formation of an antigen-antibody complex which is labeled with fluorescein-conjugated anti-immunoglobulin antibody. (From Bennington, Saunders Dictionary & Encyclopedia of Laboratory Medicine and Technology, 1984)Predictive Value of Tests: In screening and diagnostic tests, the probability that a person with a positive test is a true positive (i.e., has the disease), is referred to as the predictive value of a positive test; whereas, the predictive value of a negative test is the probability that the person with a negative test does not have the disease. Predictive value is related to the sensitivity and specificity of the test.Down-Regulation: A negative regulatory effect on physiological processes at the molecular, cellular, or systemic level. At the molecular level, the major regulatory sites include membrane receptors, genes (GENE EXPRESSION REGULATION), mRNAs (RNA, MESSENGER), and proteins.Prospective Studies: Observation of a population for a sufficient number of persons over a sufficient number of years to generate incidence or mortality rates subsequent to the selection of the study group.Genome, Viral: The complete genetic complement contained in a DNA or RNA molecule in a virus.Bacteriophage T7: Virulent bacteriophage and type species of the genus T7-like phages, in the family PODOVIRIDAE, that infects E. coli. It consists of linear double-stranded DNA, terminally redundant, and non-permuted.Chlamydia trachomatis: Type species of CHLAMYDIA causing a variety of ocular and urogenital diseases.DNA Methylation: Addition of methyl groups to DNA. DNA methyltransferases (DNA methylases) perform this reaction using S-ADENOSYLMETHIONINE as the methyl group donor.Mycoplasma Infections: Infections with species of the genus MYCOPLASMA.Cat Diseases: Diseases of the domestic cat (Felis catus or F. domesticus). This term does not include diseases of the so-called big cats such as CHEETAHS; LIONS; tigers, cougars, panthers, leopards, and other Felidae for which the heading CARNIVORA is used.Bacteriological Techniques: Techniques used in studying bacteria.Digoxigenin: 3 beta,12 beta,14-Trihydroxy-5 beta-card-20(22)-enolide. A cardenolide which is the aglycon of digoxin. Can be obtained by hydrolysis of digoxin or from Digitalis orientalis L. and Digitalis lanata Ehrh.Genes, ras: Family of retrovirus-associated DNA sequences (ras) originally isolated from Harvey (H-ras, Ha-ras, rasH) and Kirsten (K-ras, Ki-ras, rasK) murine sarcoma viruses. Ras genes are widely conserved among animal species and sequences corresponding to both H-ras and K-ras genes have been detected in human, avian, murine, and non-vertebrate genomes. The closely related N-ras gene has been detected in human neuroblastoma and sarcoma cell lines. All genes of the family have a similar exon-intron structure and each encodes a p21 protein.Temperature: The property of objects that determines the direction of heat flow when they are placed in direct thermal contact. The temperature is the energy of microscopic motions (vibrational and translational) of the particles of atoms.Mice, Inbred C57BLFatal Outcome: Death resulting from the presence of a disease in an individual, as shown by a single case report or a limited number of patients. This should be differentiated from DEATH, the physiological cessation of life and from MORTALITY, an epidemiological or statistical concept.Microsatellite Repeats: A variety of simple repeat sequences that are distributed throughout the GENOME. They are characterized by a short repeat unit of 2-8 basepairs that is repeated up to 100 times. They are also known as short tandem repeats (STRs).

*  Evaluation of real time polymerase chain reaction in tubercular mediastinal...
We studied the sensitivity of Real Time Polymerase Chain Reaction (RT-PCR) on samples ... Evaluation of real time polymerase chain reaction in tubercular mediastinal ... Evaluation of real time polymerase chain reaction in tubercular mediastinal ... Evaluation of real time polymerase chain reaction in tubercular mediastinal ......
http://erj.ersjournals.com/content/40/Suppl_56/P1413
*  Polymerase Chain Reaction (PCR): Basic Principle
... Polymerase chain reaction (PCR) is a ... Polymerase Chain Reaction (PCR): Basic Principle. *The Measurement of Tryptophan Content ... in which the temperature of the reaction is adjusted to the optimum for Taq polymerase ... After denaturation, the reaction is quickly cooled, preventing immediate reannealing of ......
http://biotechmethods.blogspot.com/2009/04/polymerase-chain-reaction-pcr-basic.html
*  Kelps feature systemic defense responses: insights into the evolution of innate...
RNA extraction and quantitative reverse transcription polymerase chain reaction (qRT-PCR) ... were quantified by quantitative reverse transcription polymerase chain reaction (RT-PCR) ... A systemic reaction was detected following elicitation on a distant area, including an ... 2005) and based on the reaction of H2O2 with cerium chloride (CeCl3) leading to insoluble ......
http://onlinelibrary.wiley.com/doi/10.1111/nph.12925/full
*  JURNAL ATANI TOKYO: Identifikasi Subtipe Virus Avian Influenza Isolat Legok...
Subtipe Virus Avian Influenza Isolat Legok Menggunakan Polymerase Chain Reaction ... Platinum Taq DNA polymerase (Invitrogen), 2 fl1 cDNA dan 38,1 µl air suling yang telah ... cDNA dilakukan dalam 50 µl campuran reaksi yang mengandung 5 µl 10X PCR reaction buffer ......
http://atanitokyo.blogspot.com/2009/12/identifikasi-subtipe-virus-avian.html
*  Endothelin B receptor gene hypermethylation in prostate adenocarcinoma |...
Methylation specific polymerase chain reaction (MSP) analysis. For PCR amplification, 2 ... Methods: Methylation specific polymerase chain reaction (MSP) for the promoter region of ... Illustrative example of methylation specific polymerase chain reaction for the EDNRB ... the GSTP1 gene in prostatic carcinoma cells detected using the polymerase chain reaction ......
http://jcp.bmj.com/content/56/1/52
*  Plus it
Total RNA Isolation and Real-Time Reverse-Transcription Polymerase Chain Reactions.. ... Tissue mRNA was measured by real-time reverse-transcription polymerase chain reaction (RT ......
http://jpet.aspetjournals.org/content/340/3/698
*  Rapid diagnosis of retina and optic nerve abnormalities in canine patients with...
Next article in issue: Fungal polymerase chain reaction testing in equine ulcerative ... Next article in issue: Fungal polymerase chain reaction testing in equine ulcerative ......
http://onlinelibrary.wiley.com/doi/10.1111/vop.12003/abstract?globalMessage=0
*  African Journal of Biotechnology - bacterial species identification getting...
... polymerase chain reaction;RFLPs, restriction fragment length polymorphisms; HTS, high- ......
http://academicjournals.org/journal/AJB/article-abstract/D0EE3FE30501
*  The Health Burden of Orphan Zoonotic Disease in the United Kingdom, 2005-2009 -...
Early Detection of Brucella Canis via Quantitative Polymerase Chain Reaction Analysis ... Early Detection of Brucella Canis via Quantitative Polymerase Chain Reaction Analysis ......
http://onlinelibrary.wiley.com/doi/10.1111/zph.12040/abstract
*  Plus it
reverse transcription-polymerase chain reaction. PSS. physiological salt solution. ACh. ... Reverse transcription-polymerase chain reaction assays on myometrial tissue demonstrated ... The resulting cDNA samples were amplified by polymerase chain reaction (PCR) using a DNA ... myosin light chain kinase. LC20. 20-kDa myosin light chain. MLCPP. myosin light chain ......
http://jpet.aspetjournals.org/content/296/3/841
*  WHO | WHO Working Group on Polymerase Chain Reaction (PCR) Protocols for Detecting Subtype Influenza
WHO. WHO Working Group on Polymerase Chain Reaction PCR Protocols for Detecting Subtype Influenza A Viruses. Skip to main content. Access Home Alt+0. Navigation Alt+1. Content Alt+2. Search Search the WHO .int site. Submit. Advanced search. Navigation Home. Health topics. Data. Media centre. Publications. Countries. Programmes. Governance. About WHO. Language عربي. 中文. English. Français. Русский. Español. RSS Feed. Youtube. Twitter. Facebook. Google +. iTunes. Play Store. Influenza. Menu. Influenza Surveillance and monitoring Updates FluID. GISRS and laboratory FluNet National Influenza Centres WHO Collaborating Centres for influenza and Essential Regulatory Laboratories WHO H5 Reference Laboratories Antiviral susceptibility surveillance WHO External Quality Assessment Project Shipping and logistic activities. PIP Framework Advisory Group Virus Sharing Benefit Sharing. Vaccines Vaccine viruses Vaccine use. Patient care Clinical management Antivirals. Human animal interface Avian influenza in humans Swine infl...
http://who.int/influenza/gisrs_laboratory/pcr_working_group/en/
*  Adaptation of the Ultrasensitive HIV-1 p24 Antigen Assay to... : JAIDS Journal of Acquired Immune
Text sizing: A. A real-time polymerase chain reaction assay for DBS DNA and/or plasma RNA identified HIV-1 infection in 38 Tanzanian children. The detection rates for the different assays were as follows: DBS-p24, 32 84% of 38 samples; DBS DNA, 30 79% of 38 samples; plasma-p24, 23 85% of 27 samples; and plasma RNA, 30 100% of 30 samples. 11-13 Specifically, in HIV-1 subtype B and C settings, p24-based diagnosis of pediatric HIV-1 infection was found to be similarly sensitive and specific as polymerase chain reaction PCR -based measurement of HIV-1 DNA or RNA. Back to Top. Back to Top. Article Outline Real-time Polymerase Chain Reaction for HIV-1 RNA RNA was extracted using the reagents of the Amplicor HIV-1 Monitor assay, version 1.5 Roche Molecular Diagnostics, Rotkreuz, Switzerland. A total of 38 subjects were identified as HIV-1 infected using the DBS HIV-1 DNA PCR assay n = 72 and/or RT-PCR assay for HIV-1 RNA in plasma n = 30. Testing of frozen plasma samples, which were not available from all 38 infecte...
http://journals.lww.com/jaids/Fulltext/2007/03010/Adaptation_of_the_Ultrasensitive_HIV_1_p24_Antigen.1.aspx
*  7900HT Fast Real-Time PCR System Support | Thermo Fisher Scientific
7900HT Fast Real-Time PCR System Support. Order Support. PCR. Real-Time PCR. Thermo Scientific. Thermo Scientific. Product Selection Guides. Services. Diagnostic Products & Services. Thermo Scientific. Diagnostic Instruments. Browse through the “Guides and Tools” section to access comprehensive product-related support resources. What volumes can be used in my plate on the 7900HT Fast Real-Time PCR System. How do I add a melt curve to my experiment on the 7900HT Fast Real-Time PCR System. How can I convert a file from AQ to RQ, or from RQ to AQ, with the 7900HT Fast Real-Time PCR System. Guide: 7900HT Fast Real-Time PCR System Maintenance and Troubleshooting Guide - Includes Calibration Guide: Relative Quantitation on 7900HT Fast Real-Time PCR System. Guide: User Guide: TaqMan® Array Micro Fluidic Cards Selection Table: Real-Time PCR Master Mixes and Instrument Compatibility. Supplemental Protocol: Rapid Cycling of TaqMan® Array Cards on 7900HT Fast Real-Time PCR System. Overview: Plastic Consumables Thermo Sc...
https://thermofisher.com/us/en/home/technical-resources/technical-reference-library/real-time-digital-PCR-instruments-support-center/7900ht-fast-real-time-pcr-system-support.html
*  Thermal Cyclers :: Alpha Laboratories
... Microplate Sealing Films. + Pipette Tips. Pipette Tips - An Overview. + Alpha Pipette Tips. Alpha Pipette Tips - An Overview. + Fastrak. Fastrak Technical Information. + Filter Pipette Tips. Filter Pipette Tips - An Overview. Non-Filter Pipette Tips. + Pipettes. Proline Pipettes. Proline Plus Pipettes. alpha+ Pipettes. + Molecular Biology Products. + Clarit-E Electrophoresis Systems. Clarit-E Electrophoresis Systems. + Horizontal Electrophoresis Systems. Midi Horizontal Electrophoresis. Midi96 Horizontal Electrophoresis System. DNA-VIEW Electrophoresis System. Maxi Horizontal Electrophoresis System. Screen Horizontal Electrophoresis System. Horizontal System Accessories. + Vertical Electrophoresis System. Vertical Electrophoresis. Mini Vertical System. Mini Wide Vertical System. Maxi Vertical System. Maxi Plus Vertical System. Maxi Z Vertical System. Vertical Modular Systems. Vertical Accessories. Electrophoresis Accessories. Molecular Biology Equipment. Thermal Cyclers by Bioer the trusted name in therm...
http://alphalabs.co.uk/product-information/molecular-biology-products/thermal-cyclers/thermal-cyclers.aspx
*  ViiA™ 7 Real-Time PCR System | Thermo Fisher Scientific
ViiA™ 7 Real-Time PCR System. Order Support. PCR. Real-Time PCR. Thermo Scientific. Thermo Scientific. Services. CaptureSelect™ Products & Services. Diagnostic Products & Services. Thermo Scientific. Diagnostic Instruments. High-performance features for maximum productivity Ideal for performing medium- to high-throughput real-time PCR, the ViiA™ 7 System enhances your lab’s productivity. Fully compatible with TaqMan Array Microfluidic Cards TaqMan Assays for real-time PCR analysis provide maximum sensitivity and specificity, and broad dynamic range. Application notes Analysis of microRNA and protein expression on a single platform using the ViiA™ 7 Real-Time PCR System Read how TaqMan Assays and the ViiA™ 7 Real-Time PCR System combine to help identify trends in miRNA and protein expression profiles on a single analytical platform. Videos Tour the instrument Take a tour through the ViiA™ 7 system and learn how features like OptiFlex™, ReadiApp™, and integrated TaqMan content can transform your lab's productiv...
http://thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/real-time-pcr-instruments/viia-7-real-time-pcr-system.html
*  LabWrench | PCR / Thermal Cyclers | Questions | Discussions | Information
PCR / Thermal Cyclers. PCR / Thermal Cyclers. Add Life Technologies Add QuantStudio® 5 Real-Time PCR System to My Bench. Life Technologies QuantStudio 3® Real-Time PCR System Now you can get up and running quickly with the Applied Biosystems® QuantStudio 3 Real-Time PCR System, a simple and affordable real-time PCR solution Add Life Technologies Add QuantStudio 3® Real-Time PCR System to My Bench. Add EPPENDORF Add Mastercycler pro to My Bench. G-Storm GS1 G-STORM GS1 Thermal Cycler Add G-Storm Add GS1 to My Bench. G-Storm GS2 The G-Storm GS2 is the perfect thermal cycler solution for laboratories that require medium to high throughput performance or for labs that have Add G-Storm Add GS2 to My Bench. Compare Equipment In PCR / Thermal Cyclers. Ltd Dynex Technologies Eagle Manufacturing Company Eberbach EDC Biosystems Edinburgh Instruments Edwards EKF Diagnostics Eksigent Elanco Eldex Electron Microscopy Sciences Electrothermal elementar ELGA Ellutia Elma Ultrasonic Cleaners EMCO High Voltage Power Corpor...
http://labwrench.com/?equipment.list/categoryNo/835/PCR---Thermal-Cyclers
*  Thermal cycler
thumb|right|A thermal cycler The 'thermal cycler' also known as a 'thermocycler', 'PCR machine' or 'DNA amplifier' is a laboratory apparatus most commonly used to amplify segments of DNA via the polymerase chain reaction PCR. 1 Thermal cyclers may also be used in laboratories to facilitate other temperature-sensitive reactions, including restriction enzyme digestion or rapid diagnostics. 2 The device has a 'thermal block' with holes where tubes holding the reaction mixtures can be inserted. History Modern innovations Pricing References External links. Rather than cycling through different temperatures, it uses three different water baths at constant temperature between which samples are moved with a robotic arm. The earliest thermal cyclers were designed for use with the Klenow fragment of DNA polymerase I. Since this enzyme is destroyed during each heating step of the amplification process, new enzyme had to be added every cycle. This led to a cumbersome machine based on an automated pipettor, with open reac...
https://en.wikipedia.org/wiki/Thermal_cycler
*  Inverse polymerase chain reaction
... thumb|right|250px|Summary of the inverse PCR process. 'Inverse polymerase chain reaction' 'Inverse PCR' is a variant of the polymerase chain reaction that is used to amplify DNA with only one known sequence. One limitation of conventional PCR is that it requires primers complementary to both termini of the target DNA, but this method allows PCR to be carried out even if only one sequence is available from which primers may be designed. Inverse PCR is especially useful for the determination of insert locations. For example, various retrovirus es and transposon s randomly integrate into genomic DNA. To identify the sites where they have entered, the known, "internal" viral or transposon sequences can be used to design primers that will amplify a small portion of the flanking, "external" genomic DNA. The amplified product can then be sequenced and compared with DNA databases to locate the sequence which has been disrupted. The inverse PCR method involves a series of restriction digest s and ligation, result...
https://en.wikipedia.org/wiki/Inverse_polymerase_chain_reaction
*  TaqMan® Gene Expression Using the QuantStudio™ 12K Flex Real-Time PCR System with OpenArray® Blo
... ck. Order Support. Real-Time PCR. Thermo Scientific. Thermo Scientific. New Products. Diagnostic Products & Services. Thermo Scientific. TaqMan Gene Expression Using the QuantStudio 12K Flex Real-Time PCR System with OpenArray Block. TaqMan® Gene Expression Using the QuantStudio™ 12K Flex Real-Time PCR System with OpenArray® Block. TaqMan® Gene Expression Using the QuantStudio™ 12K Flex Real-Time PCR System with OpenArray® Block. TaqMan OpenArray gene expression workflow on QuantStudio™ 12K Flex System. QuantStudio™ TaqMan OpenArray Real-Time PCR Plates are delivered with assays dried down in the through-holes you specify. Prepare your sample mix: Mix cDNA or the product of your pre-amplification step with TaqMan® OpenArray® Genotyping Master Mix in a TaqMan® OpenArray® 384-well sample plate. TaqMan® OpenArray® Real-Time Master Mix 1.5 mL, 2X TaqMan® OpenArray® Real-Time Master Mix 5 mL, 2X OpenArray® 384-Well Sample Plates OpenArray® 384-Well Barcoded Sample Plates. Load your sample mixes onto the TaqMan...
http://thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/real-time-pcr-applications/gene-expression-using-real-time-pcr/taqman-gene-expression-openarray.html
*  Lysis Buffer | Bio-Rad
In most purification protocols for nucleic acids, proteins, membranes, or organelles, the first step is usually cell lysis. The type of lysis buffer used depends on the types and source of cells, the desired final molecule or structure, and the level of their functionality. The protocols and lysis buffers for proteins are generally different from the lysis buffers used for nucleic acids. There are a number of different types of lysis buffer for protein extraction. The type of lysis buffer used depends on the cell source tissue culture, plant, bacteria, fungi, etc. , and whether the cells are in a structure and the type of structure. For instance, lysis of cells in tissue culture is much easier than lysis of cells in a tissue with a high level of contractile proteins such as skeletal muscle. Most lysis buffers for extraction of proteins, membranes, and organelles contain one or more detergents. Lysis Buffer for Nucleic Acids. Lysing cells for extraction of nucleic acids is in some ways much easier than extract...
http://bio-rad.com/featured/en/lysis-buffer.html
*  A consensus on fungal polymerase chain reaction diagnosis?: A United Kingdom-Ireland evaluation of
a consensus on fungal polymerase chain reaction diagnosis a united kingdom ireland evaluation of polymerase chain reaction methods for detection of systemic fungal infections westminsterresearch home about browse browse by year browse by research community browse by type browse by people browse by full text advanced search latest additions login repository administrators only statistics a consensus on fungal polymerase chain reaction diagnosis a united kingdom ireland evaluation of polymerase chain reaction methods for detection of systemic fungal infections white p lewis and barton richard and guiver malcolm and linton christopher j and wilson steve and smith melvyn and gomez beatriz l and carr michael j and kimmitt patrick t and seaton shila and rajakumar kumar and holyoake tessa and kibbler chris c and johnson elizabeth and hobson richard p and jones brian and barnes rosemary a a consensus on fungal polymerase chain reaction diagnosis a united kingdom ireland evaluation of polymerase chain reaction methods...
http://westminsterresearch.wmin.ac.uk/3185/
*  Non Food Items
... Organic Kingdom. Contact Us. Shopping Cart. Checkout. Non Food Items. Non Food Items. Items 1 to 20 of 141 total Show. Yogurt Making Thermometer Product Size: 1 each $8.50. Add to Cart Yogurt Making Thermometer Learn More. Extra Batch Jar w/lid Product Size: 1 each $16.99. Add to Cart Extra Batch Jar w/lid Learn More. Personal Blender - PB150 Product Size: 1 each $103.75. Add to Cart Personal Blender - PB150 Learn More. Sproutmaster Triple Product Size: 1 set $52.99. Add to Cart Sproutmaster Triple Learn More. Sproutmaster Single Product Size: 1 set $20.75. Add to Cart Sproutmaster Single Learn More. Crew Socks, Organic, Natural 8-10 Product Size: 1 pr. Add to Cart Crew Socks, Organic, Natural 8-10 Learn More. Crew Socks, Organic, Natural 10-13 Product Size: 1 pr. Add to Cart Crew Socks, Organic, Natural 10-13 Learn More. Learn More. Natural Paper Towels Product Size: Case/30 $104.50. Add to Cart Natural Paper Towels Learn More. Natural Paper Towels Product Size: 1 Roll $3.75. Add to Cart Natural Paper T...
http://organickingdom.com/non-food-items.html?dir=desc&limit=20&mode=list&order=position
*  User:Bill Flanagan - OpenWetWare
... My name is Bill Flanagan. 5.2 Annotation 5.2.1 Microsoft: Shared Annotation Notification Paper 5.2.2 Online Protocol Annotation 5.2.3 One Big Lab 5.2.4 Neo Note 5.2.5 PDF Annotation 5.2.6 Wired 5.2.7 Information seeking behavior of academic scientists 5.2.8 Biomedical Searching. The tools are available as no-cost downloads to academic researchers and scientists at http://research.microsoft.com/en-us/collaboration/tools. Project Trident was developed by Microsoft Research's External Research Division specifically to support the scientific community. Its goals are to enhance the user experience on computing devices, reduce the cost of writing and maintaining software, and invent novel computing technologies. showpdf http://research.microsoft.com/pubs/64568/tr-2002-74.pdf /showpdf. showpdf http://research.microsoft.com/pubs/69880/tr-2001-87.pdf /showpdf. Posted Dec 2, 2004 19:02 UTC Thu by d.e.cox guest, #3912 Parent article: The Grumpy Editor's Guide to PDF Viewers. Perkin Elmer GeneAmp 2400 PCR System The...
http://openwetware.org/index.php?title=User:Bill_Flanagan&diff=prev&oldid=449862
*  Cambio - Excellence in Molecular Biology
... Products. Custom Aptamer Synthesis. Cell culture contamination control. Detection. Elimination. Misc Products. Human. Detection kits and Reagents. Cytotoxicity Kits. Cytotoxicty Single Parameter Kits. Cytotoxicty Multiple Parameter Kits. DNA. Whole DNA. Sonicated DNA. DNA & RNA Isolation and purification kits. DNA. RNA. Protein. Media. DNA Ladders & Dyes. DNA ladders. DNA & RNA Sequencing. DNA Polymerases. Kits. Proteinases. Sequencing Utilities. Next Generation Sequencing. Sequencing Sample Prep Kits. Enzymes for Molecular Biology. Enzymes for Molecular Biology. Restriction Enzymes. Equipment. Homogenisation Equipment. Transilluminators and Light boxes. Thermal Cyclers. Vacuume Manifold. Gel Electrophoresis. Gel Electrophoresis. Nucleic Acid Ladders. Microarrays. In vitro Transcription. False negative PCR results are highly critical and might be caused by a defective PCR cycler. The Traditional Thermal Cycler Validation Kit provides temperature-sensitive PCR reactions to monitor an upper and lower tempe...
http://cambio.co.uk/1381/19/products/thermal-cycler-validation-kits/
*  U Mazza - ResearchGate
... For full functionality of ResearchGate it is necessary to enable JavaScript. Here are the instructions how to enable JavaScript in your web browser. U Mazza. Università degli Studi di Torino, Torino, Piedmont, Italy. Are you U Mazza. Claim your profile. Publications 114 420.48 Total impact. Article:. Distribution of Kaposi's sarcoma herpesvirus sequences among lymphoid malignancies in Italy and Spain. Cristina Pastore. Annunziata Gloghini. Gisella Volpe. Josep Nomdedeu. Eugenio Leonardo Umberto Mazza. Giuseppe Saglio. Antonino Carbonb. Gianluca Gaidano. ABSTRACT: In this study we have tested the distribution of Kaposi's sarcoma herpesvirus KSHV DNA sequences throughout the spectrum of lymphoid neoplasia in Italy and Spain. 180 cases of lymphoid malignancies representative of the major histologic and immunophenotypic categories of B- and T-cell tumours were analysed by means of a polymerase chain reaction-based assay. KSHV sequences were consistently absent in all categories of lymphoid malignanci...
http://researchgate.net/researcher/38344510_U_Mazza
*  Introduction To Organic Laboratory Techniques : a Microscale Approach (4TH 07 - Old Edition) by Dona
Introduction To Organic Laboratory Techniques : a Microscale Approach 4TH 07 - Old Edition by Donald L. Introduction To Organic Laboratory Techniques : a Microscale Approach 4TH 07 - Old Edition by Donald L. He is the coauthor of two organic laboratory books that include techniques and experiments: INTRODUCTION TO ORGANIC LABORATORY TECHNIQUES: A MICROSCALE APPROACH Cengage Learning, and A SMALL SCALE APPROACH TO ORGANIC LABORATORY TECHNIQUES Cengage Learning, as well as MICROSCALE AND MACROSCALE TECHNIQUES IN THE ORGANIC LABORATORY Cengage Learning, which highlights techniques to be used with a faculty member's own experiments. He is the coauthor of two organic laboratory books that include techniques and experiments: INTRODUCTION TO ORGANIC LABORATORY TECHNIQUES: A MICROSCALE APPROACH Cengage Learning, and A SMALL SCALE ARPPROACH TO ORGANIC LABORATORY TECHNIQUES Cengage Learning, as well as MICROSCALE AND MACROSCALE TECHNIQUES IN THE ORGANIC LABORATORY Cengage Learning, which highlights techniques to be use...
https://powells.com/biblio?isbn=9780495016304
*  Lysis buffer
... a lysis buffer is a buffer solution used for the purpose of lysing cells for use in molecular biology experiments that analyze the compounds of the cells e g western blot most lysis buffers contain salts e g tris hcl or edta to regulate the acidity and osmolarity of the lysate while detergents such as triton x or sds are added to break up membrane structures in studies like dna fingerprinting the lysis buffer is used for dna isolation dish soap can be used in a pinch to break down the cell and nuclear membranes allowing the dna to be released other such lysis buffers include the proprietary qiagen product buffer p ripa buffer is another commonly used lysis buffer for immunoprecipitation and general protein extraction from cells and tissues category laboratory techniques category cell biology category dna...
https://en.wikipedia.org/wiki/Lysis_buffer
*  METHODS FOR DETERMINING A PATIENT'S SUSCEPTIBILITY OF CONTRACTING A NOSOCOMIAL INFECTION AND FOR EST
In a preferred embodiment of the method of the invention the specific reagent comprises at least one amplification primer or at least one hybridization probe specific to the expression product of the S100A9 target gene and/or of the S100A8 target gene; or at least one antibody specific to the expression product of the S100A9 target gene and/or of the S100A8 target gene. The reagent can comprise at least one hybridization probe specific to the S100A9 target gene, at least one hybridization probe specific to the S100A8 target gene, at least one amplification primer specific to the S100A9 target gene, at least one amplification primer specific to the S100A8 target gene, at least one antibody specific to the molecule S100A9 or at least one antibody specific to the molecule S100A8. In a preferred embodiment of the method of the invention the specific reagent comprises at least one amplification primer or at least one hybridization probe specific to the expression product of the S100A9 target gene and/or of the S10...
http://freepatentsonline.com/y2011/0250592.html
*  Ready-to-Use Mixes, Standard PCR, Molecular Biology, Products & Ordering, Jena Bioscience
Ready-to-Use Mixes, Standard PCR, Molecular Biology, Products Ordering, Jena Bioscience. Contact Contact us How to find us Jena Bioscience at Meetings and Conferences. Products Ordering. Standard PCR. Your are here: Products Ordering / Molecular Biology / Standard PCR / Ready-to-Use Mixes. Standard PCR - Ready-to-Use Mixes. Ready-to-Use Mixes. Ready-to-Use Mixes. Ready-to-Use Mixes contain all reagents required for PCR except template and primer in a premixed 5x concentrated solution in one tube. Ready-to-Use Mixes for direct gel loading additionally contain an inherent red dye. PCR reaction products are thus ready-to-load onto agarose or acrylamide gels. Red Load Taq Master Master mix for direct gel loading. Product Cat. Amount Price EUR Buy / Note. S pack. PCR-108S. 1 ml 5x conc. L pack. PCR-108L. 5 x 1 ml 5x conc. Red Load Taq Master / high yield Master mix for direct gel loading. Product Cat. Amount Price EUR Buy / Note. S pack. PCR-106S. 1 ml 5x conc. L pack. PCR-106L. 5 x 1 ml 5x conc. Store at 2 to 8°C...
http://jenabioscience.com/cms/sid-257af7adbadbd863fa5a01c307848fcd/1/catalog/769_readytouse_mixes__direct_gel_loading.html
*  Whitepapers from Roche Diagnostics
... White Paper Roche Diagnostics. 26-Sep-2007 Transfection of cells is one of the main techniques used to influence gene expression. Real-Time Multiplex PCR of five Different DNA Targets Using the LightCycler® 480 System. Mutation Scanning of the Cytidine Deaminase Gene by High-Resolution Melting Curve Analysis Using the LightCycler® 480 System. 05-Sep-2007 The Genome Sequencer FLX System from 454 Life Sciences and Roche Applied Science is a versatile sequencing platform suitable for a wide range of applications, including de novo sequencing and assembly of genomic DNA, transcriptome sequencing, small R more. Real-Time PCR Quantification of Plant miRNAs Using Universal ProbeLibrary Technology. Real-Time PCR Quality Control for Gene Expression. Profiling Using the LightCycler® 480 System 28-Aug-2007 Quantitative real-time PCR qPCR has become the de facto standard for nucleic acid quantification. New Application for the LightCycler® 480 System: Gene Scanning by High-Resolution Melting. 28-Aug-2007 High-resolu...
http://analytica-world.com/en/whitepapers/companies/roche-diagnostics/
*  BME103:T130 Group 17 l2 - OpenWetWare
... BME103:T130 Group 17 l2 From OpenWetWare Difference between revisions Jump to: navigation, search Revision as of 00:56, 29 November 2012 view source Ricardo Robles Talk. Revision as of 00:57, 29 November 2012 view source Ricardo Robles Talk. + + ] ] !--- Include an illustration that shows how your system's primers allow specific amplification of the disease-related SNP ---. !--- Include an illustration that shows how your system's primers allow specific amplification of the disease-related SNP ---. Lab Write-Up 1. Lab Write-Up 2. Lab Write-Up 3. 1 GROUP 17 2 LAB 2 WRITE-UP: Redesigning Open PCR for Autism Detection 2.1 Thermal Cycler Engineering 2.2 Protocols 2.3 Research and Development. Key Features The first picture shows the heating block that is in use in the current PCR machine. The picture below is that of the current cooling system. Instructions 1 Remove the current heating block and cooling system from the PCR machine. 2 Replace removed items with the larger heating block and larger cooling syst...
http://openwetware.org/index.php?title=BME103:T130_Group_17_l2&diff=660468&oldid=660466
*  Search results for: 'DISC1' - Aviva Systems Biology
Disease Categories. DISC1 antibody - C - terminal region OAAB11126 Protein Name: Disrupted in schizophrenia 1 protein Catalog #: OAAB11126 Type: pAb Reacts with: Human Application: WB, FC, IHC Product Size: 400ul Price USD : $289.00. DISC1 Antibody OAPB00558 Protein Name: Translin-associated protein X Catalog #: OAPB00558 Type: pAb Reacts with: Human; Mouse Application: ELISA, WB, ICC Product Size: 0.1 mg Price USD : $375.00. DISC1 antibody - N-terminal region ARP47934 P050 Protein Name: Disrupted in schizophrenia 1 protein Catalog #: ARP47934 P050 Type: pAb Reacts with: Horse; Human; Mouse; Rabbit; Rat Application: WB Product Size: 100 ul Price USD : $289.00. TSNAX antibody - N - terminal region OAAB10245 Protein Name: Translin-associated protein X Catalog #: OAAB10245 Type: pAb Reacts with: Human; Mouse; Rat Application: WB, IHC Product Size: 400ul Price USD : $289.00. DISC1 antibody OAAB07113 Protein Name: Disrupted in schizophrenia 1 protein Catalog #: OAAB07113 Type: mAb Reacts with: Human Application: W...
http://avivasysbio.com/catalogsearch/result/?q=DISC1&ra=146&re=Zebra Fish&pt=77
*  Search results for: 'DISC1' - Aviva Systems Biology
Disease Categories. DISC1 antibody - C - terminal region OAAB11126 Protein Name: Disrupted in schizophrenia 1 protein Catalog #: OAAB11126 Type: pAb Reacts with: Human Application: WB, FC, IHC Product Size: 400ul Price USD : $289.00. DISC1 Antibody OAPB00558 Protein Name: Translin-associated protein X Catalog #: OAPB00558 Type: pAb Reacts with: Human; Mouse Application: ELISA, WB, ICC Product Size: 0.1 mg Price USD : $375.00. DISC1 antibody - N-terminal region ARP47934 P050 Protein Name: Disrupted in schizophrenia 1 protein Catalog #: ARP47934 P050 Type: pAb Reacts with: Horse; Human; Mouse; Rabbit; Rat Application: WB Product Size: 100 ul Price USD : $289.00. DISC1 antibody OAAB07113 Protein Name: Disrupted in schizophrenia 1 protein Catalog #: OAAB07113 Type: mAb Reacts with: Human Application: WB Product Size: 400ul Price USD : $289.00. DISC1 Antibody OAPB00557 Protein Name: Translin-associated protein X Catalog #: OAPB00557 Type: pAb Reacts with: Human; Mouse Application: ELISA, WB Product Size: 0.1 mg Pr...
http://avivasysbio.com/catalogsearch/result/?q=DISC1&ra=146&single=d&ho=Rabbit
*  The Methods January 1992 Archive by author
Ericomp thermal cycler BACKD at QUCDN.QueensU.CA Multi bin feeder BIOCUKM at OSUCC.bitnet PCR/Sequencing Kit from ABI BMYCHERN at imb.as.tw Tet selection problems in pBR322 Michael Benedik Gene Fusions Michael Benedik PCR Hirdeypal S. Anthony Davies Promega Magic PCR Preps kit Dan Diaz Semipreparative 10's of micrograms PCR Dan Diaz Taq Ligase and LDR Dan Diaz gel dryer. Paul Hengen Cloning PCR products Brian Hjelle Hirt Supernatents for Cloning Viral DNA Brian Hjelle PCR Mutagenesis -Replacement of 5 bp Brian Hjelle PCR with primers of different lengths IO00865 at MAINE.MAINE.EDU microtube homogeniser. Muriana Tet selection problems in pBR322 John Nash Increasing PCR specificity with formamide John Nash Cloning PCR products Pamela Norton Subscription to Methods F. Todd Richmond System7 and Laser Question Bruce Roe Vector Sequences in the Databases Bruce Roe PCR/Sequencing Kit from ABI Bruce Roe Ericomp Thermal Cyclers Dan Rohwer-Nutter Ericomp favorable opinion. Smith Cloning PCR products Michael W. Templeto...
http://bio.net/bionet/mm/methods/1992-January/author.html
*  CST - SimpleChIP® Human NR4A3 Promoter Primers
... Related Products. SimpleChIP ® Enzymatic Chromatin IP Kit Agarose Beads. SimpleChIP ® Enzymatic Chromatin IP Kit Magnetic Beads. PRINT SimpleChIP ® Human NR4A3 Promoter Primers #4829. SimpleChIP ® Human NR4A3 Promoter Primers were tested on DNA isolated from cross-linked cells using the SimpleChIP ® Enzymatic Chromatin IP Kit Magnetic Beads #9003. Real-time PCR was performed in duplicate on a serial dilution of 2% total input DNA 20 ng, 4 ng, 0.8 ng, and 0.16 ng using a real-time PCR detection system and SYBR ® Green reaction mix. PCR product melting curves were obtained on Real-Time PCR reactions performed using SimpleChIP ® Human NR4A3 Promoter Primers. Data is shown for both duplicate PCR reactions using 20 ng of total DNA. Gallery: SimpleChIP ® Human NR4A3 Promoter Primers #4829. SimpleChIP ® Human NR4A3 Promoter Primers were tested on DNA isolated from cross-linked cells using the SimpleChIP ® Enzymatic Chromatin IP Kit Magnetic Beads #9003. Real-time PCR was performed in duplicate on a serial dilut...
http://cellsignal.com/products/chip-kits-reagents/human-nr4a3-promoter-primers/4829?N=4294967123&Nrpp=30&No=30&fromPage=plp
*  Molecular Methods for Virus Detection | 978-0-12-748920-9 | Elsevier
Molecular Methods for Virus Detection. Elsevier. Molecular Methods for Virus Detection Edited by Danny Wiedbrauk, William Beaumont Hospital, Royal Oak, Missouri, U.S.A. Daniel Farkas, William Beaumont Hospital, Royal Oak, Michigan, U.S.A. Molecular diagnostic procedures have been described in a number of recent books and articles. However, these publications have not focused on virus detection, nor have they provided practical protocols for the newer molecular methods.Written by the inventors or principal developers of these technologies, Molecular Methods for Virus Detection provides both reviews of individual methods and instructions for detecting virus nucleic acid sequences in clinical specimens. Each procedure includes quality assurance protocols that are often ignored by other methodology books. Molecular Methods for Virus Detection provides clinically relevant procedures for many of the newer diagnostic methodologies. Audience Researchers, graduate students, technicians, and clinicians in virology, mic...
http://elsevier.com/books/molecular-methods-for-virus-detection/wiedbrauk/978-0-12-748920-9
*  DNAase step in differential display PCR
... william sun william at neuro usc edu fri jun est previous message synthesis method for thiazole orange next message dnaase step in differential display pcr messages sorted by after isolation of mrna for differential display pcr is it necessary to treat the mrna with dnaase i would like to omit extra steps if possible to prevent rnaase contamination william william sun ph d phone neuroscience program fax university of southern california pager los angeles ca http rana usc edu wisun previous message synthesis method for thiazole orange next message dnaase step in differential display pcr messages sorted by more information about the methods mailing list...
http://bio.net/bionet/mm/methods/1996-June/045453.html
*  Supplements, Standard PCR, Molecular Biology, Products & Ordering, Jena Bioscience
Supplements, Standard PCR, Molecular Biology, Products Ordering, Jena Bioscience. Standard PCR. Your are here: Products Ordering / Molecular Biology / Standard PCR / Supplements. Standard PCR - Supplements. Standard PCR Supplements. Gel Loading Buffer with DNA Stain Loading buffer for agarose or polyacrylamide gels with EvaGreen fluorescent DNA stain. Product Cat. Amount Price EUR Buy / Note. PCR-255-or. Gel Loading Buffers with DNA Stain are formulated to facilitate loading of DNA samples into the wells of agarose and polyacrylamide gels. The loading buffers contain EvaGreen ® DNA Stain a fluorescent DNA intercalator dye specially developed for DNA analysis applications. Gel Loading Buffer Loading buffer for agarose or polyacrylamide gels. Product Cat. Amount Price EUR Buy / Note. PCR-254-or. Gel Loading Buffers are formulated to facilitate loading of DNA containing samples into the wells of agarose and polyacrylamide gels. Green Gel Loading Buffer contains orange G and xylene cyanol FF as tracking dyes and ...
http://jenabioscience.com/cms/sid-61f7b6556b8d4d2c2ab733f2196dbca2/1/catalog/712/?m=1&k=8e
*  Supplements, Standard PCR, Molecular Biology, Products & Ordering, Jena Bioscience
Supplements, Standard PCR, Molecular Biology, Products Ordering, Jena Bioscience. Standard PCR. Your are here: Products Ordering / Molecular Biology / Standard PCR / Supplements. Standard PCR - Supplements. Standard PCR Supplements. Gel Loading Buffer with DNA Stain Loading buffer for agarose or polyacrylamide gels with EvaGreen fluorescent DNA stain. Product Cat. Amount Price EUR Buy / Note. PCR-255-or. Gel Loading Buffers with DNA Stain are formulated to facilitate loading of DNA samples into the wells of agarose and polyacrylamide gels. The loading buffers contain EvaGreen ® DNA Stain a fluorescent DNA intercalator dye specially developed for DNA analysis applications. Gel Loading Buffer Loading buffer for agarose or polyacrylamide gels. Product Cat. Amount Price EUR Buy / Note. PCR-254-or. Gel Loading Buffers are formulated to facilitate loading of DNA containing samples into the wells of agarose and polyacrylamide gels. Green Gel Loading Buffer contains orange G and xylene cyanol FF as tracking dyes and ...
http://jenabioscience.com/cms/sid-87b41f021e15955ec8f65a6d7fa86023/1/catalog/712/?m=1&k=39
*  MassTag-PCR
masstag pcr masstag pcr masstag pcr is a new technology pcr based on mass spectrometric detection of end product this technology is pioneered by scientist from center for infection and immunity cii of mailman school of public health at columbia university usa principles like conventional pcr masstag pcr uses primer pairs the difference is primers used for masstag pcr are tagged with molecules of known masses or masscodes instead of single pair of primers this technology uses a number of primers making it a multiplex system unlike conventional multiplex pcr system in masstag pcr more than primer pairs could be used if dna from any of the agent of primer panel is present it will be amplified each amplified product will carry its specific masscodes the pcr product is then purified to remove unbound primers dntps enzyme and other impurities finally the purified pcr products are subject of uv as the chemical bond with nucleic acid and primers are photolabile as the masscodes are liberated from pcr products they ar...
https://en.wikipedia.org/wiki/MassTag-PCR
*  Healthy Supplements | Organic Supplements
Healthy Supplements. Organic Supplements. JavaScript seem to be disabled in your browser. You must have JavaScript enabled in your browser to utilize the functionality of this website. Organic Kingdom. Your Account. Your Wishlist. Contact Us. Shopping Cart. Checkout. Login. Home /. Supplements. Supplements. Items 10 to 18 of 428 total Show. 9. 15. 30. All. per page Page:. 1 2. 3 4. 5. View as: List Grid Sort By. Position. Name. Price. Klamath Shores Blue Green Algae Product Size: 60 caps $15.99. Add to Cart. Add to Wishlist. Add to Compare. More Than A Multiple Product Size: 120 tabs $29.50. Add to Cart. Add to Wishlist. Add to Compare. Potent Acidophilus Supplement Product Size: 100 caps $9.50. Add to Cart. Add to Wishlist. Add to Compare. Royal Brittany Evening Primrose Oil Product Size: 100 softgels + $16.99. Add to Cart. Add to Wishlist. Add to Compare. Salmon Oil Product Size: 120 softgels $7.99. Add to Cart. Add to Wishlist. Add to Compare. American Native Herbal TABLETS Product Size: 60 tabs $55.99. Ad...
http://organickingdom.com/supplements.html?dir=asc&limit=9&mode=grid&order=position&p=2
*  In Their Own Words
... Office of NIH History. Polymerase Chain Reaction Test. Diagram of the polymerase chain reaction test PCR shows how HIV DNA sequences as a way to diagnose HIV infection in blood samples. return. Office of NIH History. NIH. DHHS....
https://history.nih.gov/NIHInOwnWords/docs/page_39g.html
*  Applications of PCR
... ::'This page assumes familiarity with the terms and components used in the polymerase chain reaction PCR process. The polymerase chain reaction PCR has found widespread application in many areas of genetic analysis. Medical applications Infectious disease applications Forensic applications Research applications External links References. Medical applications. was for ' genetic testing ', where a sample of DNA is analyzed for the presence of genetic disease mutations. Prospective parents can be tested for being genetic carrier s, or their children might be tested for actually being affected by a disease. PCR analysis is also essential to preimplantation genetic diagnosis, where individual cells of a developing embryo are tested for mutations. Infectious disease applications. Characterization and detection of infectious disease organisms have been revolutionized by PCR:. The earliest tests for infection relied on the presence of antibodies to the virus circulating in the bloodstream. However, antibodies do...
https://en.wikipedia.org/wiki/Applications_of_PCR
*  KI and DNA purification
... dr duncan clark duncan at nospam demon co uk tue mar est previous message ki and dna purification next message ki and dna purification messages sorted by in article bpmurray stuffer at macmac ucsf edu bernard p murray phd bpmurray stuffer socrates ucsf edu writes i have inherited a protocol for purifying dna from agarose gels with promega s agarace enzyme the protocol i have calls for melting the gel slices in m ki final conc m in ul total volume before adding the agarace thanks jennifer is that really ki i thought most protocols user periodate kio or perchlorate for dissolution of agarose sorry to question you its just that the idea of using ki is new to me don t know about ki but m nai with g l na sulphite works fine store in the dark or an opaque bottle etc if it is stored in the light it will go yellow duncan the problem with being on the cutting edge is that you occasionally get sliced from time to time duncan clark dnamp ltd tel fax http www dnamp com http www genesys demon co uk previous message k...
http://bio.net/bionet/mm/methods/1999-March/074628.html
*  Videos for polymerase chain reaction - Homework Help Videos - Brightstorm
videos for polymerase chain reaction homework help videos brightstorm toggle navigation browse subjects math pre algebra algebra geometry algebra trigonometry precalculus calculus science biology chemistry physics english grammar writing literature test prep sat act act red book psat ap us gov ap us history ap biology ap calculus ab college get better grades college application college essay financial aid search or find by textbook study math science english test prep sign in teacher membership school membership start your free trial videos for polymerase chain reaction pcr dna fingerprinting science biology molecular biology the pcr method of dna fingerprinting tags pcr dna fingerprinting gel electrophoresis polymerase chain reaction about how to use about us our teachers for schools jobs press webinars ebooks support faq library math pre algebra algebra geometry algebra trigonometry precalculus calculus science biology chemistry physics english grammar writing literature test prep sat act act red book psat ...
http://brightstorm.com/tag/polymerase-chain-reaction/
*  Search results for: 'SLC2A5' - Aviva Systems Biology
... Polyclonal Antibodies. Cell Cycle Proteins Antibodies. Membrane Protein Antibodies. RNA Binding Proteins Antibodies. Membrane Protein Antibodies. Protein. DNA/cDNA/RNA. Kits. ELISA Kits. Other Kits. RT PCR Pairs. Tissues Cells. By Gene. Apoptosis. Cancer. Cell Biology. Chromatin Nuclear Signaling. Disease Related. DNA Damage Repair. DNA Repair. DNA/RNA/Protein Interactions. Stem Cells. Transcription Factors. Disease Categories. Filter by Product Name: Show All A B C D E F G H I J K L M N O P Q-R S T U V W Y Z 0-1 2-4 5 6 7-9. SLC2A5 Antibody ARP42096 P050 Protein Name: Solute carrier family 2, facilitated glucose transporter member 5 Catalog #: ARP42096 P050 Type: pAb Reacts with: Cow; Dog; Guinea Pig; Horse; Human; Mouse; Rat; Sheep Application: IF, WB Product Size: 100 ul Price USD : $289.00. GLUT5 Polyclonal Antibody OABB00689 Protein Name: Solute carrier family 2, facilitated glucose transporter member 5 Catalog #: OABB00689 Type: pAb Reacts with: Human; Mouse; Rat Application: WB IHC-P Product Size:...
http://avivasysbio.com/catalogsearch/result/?q=SLC2A5&pt=47&cl=Monoclonal
*  relative expression of genes using semiquantitative PCR - PCR, RT-PCR and Real-Time PCR - BioForum
... Google Sign in options. Remember me This is not recommended for shared computers Sign in anonymously Don't add me to the active users list Privacy Policy. Sign In. Create Account. This topic. Forums. Members. BioBlog. BioWiki. Calendar. Quotes. BioVideo. Forums. BioBlog. BioWiki. Calendar. Quotes. Contact Us. BioForum. Protocols and Techniques Forums. PCR, RT-PCR and Real-Time PCR. Javascript Disabled Detected You currently have javascript disabled. relative expression of genes using semiquantitative PCR Started by. Please log in to reply. 1 reply to this topic #1. nirupakl. nirupakl. member Active Members. 11 posts. First of all would like to apolgise for posting a question on semi-quantitative PCR in the era of real-time PCR. I have analyzed expression of three genes: a, b and c in certain pathological tissues. I have performed agarose gel electrophoresis and have the gels analyzed by Bio-Rad gel documentation system. i have seen some papers which say that they have seen relative expression with respec...
http://protocol-online.org/forums/topic/21976-relative-expression-of-genes-using-semiquantitative-pcr/
*  CST - SimpleChIP® Mouse MYT-1 Promoter Primers
... Related Products. SimpleChIP ® Enzymatic Chromatin IP Kit Agarose Beads. SimpleChIP ® Enzymatic Chromatin IP Kit Magnetic Beads. PRINT SimpleChIP ® Mouse MYT-1 Promoter Primers #8985. Products. SimpleChIP ® Mouse MYT-1 Promoter Primers were tested on DNA isolated from cross-linked cells using the SimpleChIP ® Enzymatic Chromatin IP Kit Magnetic Beads #9003. Real-time PCR was performed in duplicate on a serial dilution of 2% total input DNA 20 ng, 4 ng, 0.8 ng, and 0.16 ng using a real-time PCR detection system and SYBR ® Green reaction mix. PCR product melting curves were obtained for real-time PCR reactions performed using SimpleChIP ® Mouse MYT-1 Promoter Primers. Data is shown for both duplicate PCR reactions using 20 ng of total DNA. Gallery: SimpleChIP ® Mouse MYT-1 Promoter Primers #8985. SimpleChIP ® Mouse MYT-1 Promoter Primers were tested on DNA isolated from cross-linked cells using the SimpleChIP ® Enzymatic Chromatin IP Kit Magnetic Beads #9003. Real-time PCR was performed in duplicate on a s...
http://cellsignal.com/products/cellular-assay-kits/8985.html
*  Genetic analysis
Basic studies include identification of genes and inherited disorders. Genetic analysis can be used generally to describe methods both used in and resulting from the sciences of genetics and molecular biology, or to applications resulting from this research. Genetic analysis may be done to identify genetic/inherited disorders and also to make a differential diagnosis in certain somatic diseases such as cancer. Genetic analyses of cancer include detection of mutation s, fusion gene s, and DNA copy number changes. 'Genetic analyses' include but are not limited to molecular technologies such as PCR, RT-PCR, DNA sequencing, and DNA microarrays, and cytogenetic methods such as karyotyping and fluorescence in situ hybridisation. DNA Sequencing. DNA sequencing is essential to the applications of genetic analysis. 'Cytogenetics' is a branch of genetics that is concerned with the study of the structure and function of the cell, especially the chromosomes Polymerase chain reaction studies the amplification of DNA. Poly...
https://en.wikipedia.org/wiki/Genetic_analysis
*  Primers PCR-Vectorette.
primers pcr vectorette primers pcr vectorette jingfang niu jniu at nmu edu wed nov est previous message oligo program next message chloroform in colony blots messages sorted by i am also trying to use the vectorette ii kit by genosys i believe genosys patented the vectorette system therefore they don t divulge the primer or vectorette sequence information if they had i would have been able to construct my own vectorette units and primers vs the universal primer listed anywhere i have already constructed my own vectorette library however i have been getting strange results so i opted to purchase a genosys kit you can run a control using only primers if you are looking for primer dimers last time i ran a vectorette reaction i ran control reactions using single primers and no template reactions surprisingly enough i got a product using only one primer my specific primer this may be due to some inversion or repeated sequences if you have any luck using vectorette ii please let me know it seems to be a tricky syst...
http://bio.net/bionet/mm/methods/1998-November/071933.html
*  Aviva Systems Biology - Reagents & Antibodies
CDK5 antibody - N-terminal region ARP38525 T100 Protein Name: Cyclin-dependent kinase 5 Catalog #: ARP38525 T100 Type: pAb Reacts with: Cow; Dog; Guinea Pig; Horse; Human; Mouse; Rabbit; Rat; Sheep; Zebrafish Application: WB Product Size: 100 ul Price USD : $229.00. CDK5 antibody - C-terminal region ARP38526 T100 Protein Name: Cyclin-dependent kinase 5 Catalog #: ARP38526 T100 Type: pAb Reacts with: Cow; Dog; Guinea Pig; Horse; Human; Mouse; Pig; Rabbit; Rat; Sheep; Yeast; Zebrafish Application: IHC, WB Product Size: 100 ul Price USD : $229.00. NCOR1 antibody - N-terminal region ARP32479 P050 Protein Name: Nuclear receptor corepressor 1 Catalog #: ARP32479 P050 Type: pAb Reacts with: Cow; Dog; Guinea Pig; Horse; Human; Mouse; Rat; Yeast Application: IHC, WB Product Size: 100 ul Price USD : $289.00. Foxa3 antibody - C-terminal region ARP36881 P050 Protein Name: Hepatocyte nuclear factor 3-gamma Catalog #: ARP36881 P050 Type: pAb Reacts with: Cow; Dog; Guinea Pig; Horse; Human; Mouse; Pig; Rat Application: WB P...
http://avivasysbio.com/catalogsearch/advanced/result/?related_component=Nuclear Part
*  DNA Isolation
... Entamoeba is known to store glycogen and standard DNA isolation methods often yield DNA that is difficult to digest with restriction enzymes or use as a template for polymerase chain reaction. Sample collection My own experience has been only with material from in vitro cultures so that readers may want to find methods for DNA isolation directly from stool or other sources elsewhere. However, I know from work by other researchers that this method has been used successfully to isolate Entamoeba DNA from stool that was a suitable template for Polymerase Chain Reaction amplification. 1 A pellet of at least 50 l packed volume can be processed with the following protocol. Add 75 l of 3.5 M NaCl, mix gently then add 42 l of 10% CTAB/0.7 M NaCl Note heated to 55 C, mix and incubate at 65 C for 20 minutes. At room temperature add 400 l of chloroform, mix well by inverting and spin at full speed in a microcentrifuge for 10 minutes. Note. Transfer the supernatant to a fresh tube and add 400 l of phenol:chloroform:...
http://entamoeba.lshtm.ac.uk/dnaisoln.htm
*  Molecular Biology, Products & Ordering, Jena Bioscience
Standard PCR. Reverse Transcription. Ready-to-Use Lyophilisates. DNA Ladders. Protein MW Marker / Blot stain. Cloning and Mutagenesis. Thermophilic Polymerases dNTP Guide qPCR with EvaGreen RNA / DNA Purification DNA Ladders Guide Restr. Standard PCR Direct and Multiplex PCR Ready-to-Use Mixes Core Kits Thermophilic Polymerases dNTP Mixes dNTP Bundles and Single Solutions Supplements School and Demo Kits Real-Time PCR qPCR Multiplex Master Mixes qPCR Master Mixes for Dual Labeled Probes qPCR Master Mixes with EvaGreen qPCR Core Kits qPCR Supplements Dual Labeled Fluorescent Probes Reverse Transcription One-Step RT-qPCR One-Step RT-PCR Two-Step RT-PCR Reverse Transcriptases Supplements in vitro Transcription in vitro Transcription Kits RNA Polymerases NTP Solutions Ready-to-Use Lyophilisates Real-Time Master Lyophilisates Direct PCR and Multiplex PCR Lyophilisates Taq Hot Start Master Lyophilisates Reverse Transcription Master Lyophilisates dNTP Lyophilisates Custom-Specific Lyophilizates Primers and Oligonucl...
http://jenabioscience.com/cms/sid-c0cf6056b1bcda3eb9ad4ee21708c41c/1/browse/109_molecular_biology.html
*  Molecular Biology, Products & Ordering, Jena Bioscience
Standard PCR. Reverse Transcription. Ready-to-Use Lyophilisates. DNA Ladders. Protein MW Marker / Blot stain. Cloning and Mutagenesis. Thermophilic Polymerases dNTP Guide qPCR with EvaGreen RNA / DNA Purification DNA Ladders Guide Restr. Standard PCR Direct and Multiplex PCR Ready-to-Use Mixes Core Kits Thermophilic Polymerases dNTP Mixes dNTP Bundles and Single Solutions Supplements School and Demo Kits Real-Time PCR qPCR Multiplex Master Mixes qPCR Master Mixes for Dual Labeled Probes qPCR Master Mixes with EvaGreen qPCR Core Kits qPCR Supplements Dual Labeled Fluorescent Probes Reverse Transcription One-Step RT-qPCR One-Step RT-PCR Two-Step RT-PCR Reverse Transcriptases Supplements in vitro Transcription in vitro Transcription Kits RNA Polymerases NTP Solutions Ready-to-Use Lyophilisates Real-Time Master Lyophilisates Direct PCR and Multiplex PCR Lyophilisates Taq Hot Start Master Lyophilisates Reverse Transcription Master Lyophilisates dNTP Lyophilisates Custom-Specific Lyophilizates Primers and Oligonucl...
http://jenabioscience.com/cms/sid-c5378fbb19c61c10c5efad01ba67454b/1/browse/109_molecular_biology.html
*  Licensing your thermocycler for PCR?
licensing your thermocycler for pcr licensing your thermocycler for pcr wschick at aol com wschick at aol com thu oct est previous message vogel bonner next message optimal running time for sscp messages sorted by i don t know of any cycler manufacturer that can license for diagnostic purposes only for research roche held back diagnostic licensing market from pe but now pe applied biosystems has announced an agreement from roche to use pcr with their fluorescent technology i guess that s taqman you should contact roche about diagnostic licensing or pcr you could start at roche molecular in alameda california ph walt schick i have no connection to roche or pe abi previous message vogel bonner next message optimal running time for sscp messages sorted by more information about the methods mailing list...
http://bio.net/bionet/mm/methods/1996-October/050917.html
*  What Assays are used in Protein Concentration Determination?
What Assays are used in Protein Concentration Determination. Protein Electrophoresis 17. Protein Purification 16. Protein Estimation 12. Protein Extraction 11. Sample Clean Up 9. Protein Concentration 8. Protein Detection 7. Assay Development ELISA 6. Apoptosis Assays 5. Protein Fractionation 4. Why do I need a protease inhibitor. Using Protease Assays for Accurate Protease Detection. Are Total Protein Membrane Stains Compatible With IR Imaging Systems. What Assays are used in Protein Concentration Determination. What assays are used in protein concentration determination. Due to the fact that there are no protein assays that are specific to any particular protein or are sensitive to all types of proteins, it is absolutely impossible to have a single assay that can give absolutely accurate results when performing protein concentration determination. To find protein assays that are most compatible with your sample, you need to consider their compatibility with the sample type and its components, the assay rang...
http://info.gbiosciences.com/blog/bid/163070/What-Assays-are-used-in-Protein-Concentration-Determination
*  The Secrets of Coupling with Biotin!
Protein Research. Protein Electrophoresis 17. Protein Purification 16. Protein Estimation 12. Protein Extraction 11. Protein Detection 7. Why do I need a protease inhibitor. Using Protease Assays for Accurate Protease Detection. Are Total Protein Membrane Stains Compatible With IR Imaging Systems. The conjugation efficiency of the reactions is dependent on the reaction groups and the buffers used for the reactions as many coupling reactions are sensitive to pH and chemical composition. Reaction Conditions. NHS esters are soluble in organic solvents and DMSO or DMF are the most commonly used, which are compatible with most proteins in a 20% solution. For optimal conjugation, we recommend Optimizer Buffer™-I. General Precautions. There are three different reactions employed to couple biotin reagents to sulfhydryl residues and involve either iodoacetyl, maleimide or pyridylthiol groups. Maleimide Reaction Conditions. The maleimide group is more specific for sulfhydryl residues than iodoacetyl groups, at pH7 male...
http://info.gbiosciences.com/blog/bid/128778/the-secrets-of-coupling-with-biotin
*  AP-PCR QUESTION
ap pcr question ap pcr question jenny williams jenova at microbes demon co uk sun jun est previous message postdoctoral position next message respirometer for measuring o co of bacteria algae messages sorted by i have recently completed a project on molecular typing using arbitrary primer pcr ap pcr this technique uses a single arbitrary primer in each pcr assay i used random primers based on published sequences which had previously been applied successfully in the typing of clostridium difficile i followed the published protocols carefully but was unable to get reproducible results i then combined the same two random primers that i had previously used in single primer pcr assays in the same reaction tube the assay conditions were identical to that of the single primer assays with the exception that primers were now taking part in the reaction also the total amount of primer in the reaction tube was doubled this combination produced reproducible results also the resulting dna profile produced after gel electr...
http://bio.net/bionet/mm/methods/1997-June/058932.html
*  PCR Techniques & Applications Workshop
Previous message: rDNA Techniques Applications Workshop Next message: gel doc/photo imaging system Messages sorted by:. POLYMERSE CHAIN REACTION PCR APPLICATIONS/ CYCLE DNA SEQUENCING. American Type Culture Collection ATCC April 21-24, 1997, Rockville, MD A four-day, laboratory-intensive, course covering a broad range of applications of the polymerase chain reaction PCR for addressing biological problems. Lecture topics will include PCR Reaction Basics, Optimizing and Troubleshooting the PCR Reaction, DNA Sequencing, PCR Analysis of Ribosomal DNA to Determine Taxonomic Relatedness, PCR Fingerprinting, PCR in the Diagnosis of Infectious and Genetic Disease, and PCR and Recombinant DNA Methodology. Laboratory exercises will include: 1 PCR Diagnosis of Sickle Cell Disease; 2 Fingerprint Analysis of the DNA of Human Individuals; 3 Relatedness of Organisms by Analysis of Amplified Ribosomal DNA; 4 Amplification from mRNA; 5 Optimization of PCR Reactions; 6 Labeling of Hybridization Probes by PCR; 7 Cycle DNA Seque...
http://bio.net/bionet/mm/methods/1997-March/055281.html
*  Lysis Buffer for Total Protein Assay - Protein Expression and Purification - BioForum
... Jump to content. Google Sign in options. Remember me This is not recommended for shared computers Sign in anonymously Don't add me to the active users list Privacy Policy. . Sign In. Create Account. Search Advanced. Search section:. This topic. Forums. Members. Help Files. BioBlog. BioWiki. Interest Groups. Calendar. Quotes. BioVideo. View New Content. Protocol Online. BioPortal. Forums. BioBlog. BioWiki. Interest Groups. Calendar. Quotes. Contact Us. More. BioForum. Protocols and Techniques Forums. Protein Expression and Purification. . Javascript Disabled Detected You currently have javascript disabled. Several functions may not work. Please re-enable javascript to access full functionality. Submit your paper to J Biol Methods today. Lysis Buffer for Total Protein Assay Started by. Lupo Comunitario, Apr 08 2009 04:54 AM. Please log in to reply. 1 reply to this topic #1. Lupo Comunitario. Lupo Comunitario. member Members. 3 posts. 1 Neutral. Posted 08 April 2009 - 04:54 AM Dear All I need to measure the...
http://protocol-online.org/forums/topic/7413-lysis-buffer-for-total-protein-assay/
*  Looking for the optimal Cell Lysis Buffer
... Lahti via methods%40net.bio.net by laht0028 from umn.edu Mon Dec 10 13:12:44 EST 2007. Previous message: how to calculate Kcat value of enzyme Next message: Rhodamine dUTP and capillary eletrophoresis Messages sorted by:. I am looking for a cell lysis buffer that will help me solubilize a lysosmal cathepsin. I am currently finding the Cathepsin in my cell lysate samples as well as my cell pellet. The cathepsin may be membrane associated with the plasma membrane or other cellular membranes. What are detergents and/or buffers that might best aid me in removing a cathepsin from my cellular debris. Lahti -- View this message in context: http://www.nabble.com/Looking-for-the-optimal-Cell-Lysis-Buffer-tp14258171p14258171.html Sent from the Bio.net - Methods mailing list archive at Nabble.com. Previous message: how to calculate Kcat value of enzyme Next message: Rhodamine dUTP and capillary eletrophoresis Messages sorted by:. More information about the Methods mailing list....
http://bio.net/bionet/mm/methods/2007-December/102750.html
*  Q: Inaccuracies in PCR sequencing and genetic variability
q inaccuracies in pcr sequencing and genetic variability q inaccuracies in pcr sequencing and genetic variability fred rickson ricksonf at bcc orst edu fri feb est previous message q inaccuracies in pcr sequencing and genetic variability next message hershey chargaff messages sorted by on thu feb daniel gautheret wrote i bet this issue has already been debated but i can t find any relevant data in the litterature so here it is direct sequencing of pcr products often yields sequences with many inaccuracies ns can we say that the position of these undefined nucleotides is related to actual genetic variation at these particular sites thank you daniel gautheret i suspect that if you are getting more than an acceptable amount of misreads then you are going to have to clone what must be a heterogeneous target area on a clean dna region i can sequence times with maybe two changes each time over bases fred rickson previous message q inaccuracies in pcr sequencing and genetic variability next message hershey chargaff ...
http://bio.net/bionet/mm/bioforum/1997-February/022671.html
*  RACE pcr product - Biology Stack Exchange
... Biology Meta. more stack exchange communities. Stack Exchange. Help Center Detailed answers to any questions you might have. Biology Questions. Biology Stack Exchange is a question and answer site for biology researchers, academics, and students. RACE pcr product. If it wanted to sequence my PCR product that might be hundreds, if not thousands of bps long, I should use RACE PCR correct. My goal is to sequence the active site of some enzymes I'm interested in, but I'm not sure how to go about designing PCR primers for a specific site on my protein. Additionally, the active site is over 100 amino acids long, which would imply that my PCR product has to be over 300 bps long. I was thnking about using RACE PCR to amplify my entire cDNA strand that is 4000 bps long and then just sequencing the RACE product. Would RACE work for that many bps in my cDNA. It would probably be best if you asked separate questions. Using these flanking sequences, two short oligonucleotides e.g., 18-24 nucleotides, called primers, ...
http://biology.stackexchange.com/questions/7593/race-pcr-product/7606
*  Development and Evolution of PCR | GEN Magazine Articles | GEN
Market & Tech Analysis. PCR the enzymatic amplification of a specific DNA fragment targeted by two oligonucleotide primers is a surprisingly simple concept but a technology that has become increasingly powerful and broadly implemented. Starting with a DNA or RNA template, repeated cycles of denaturation, primer annealing, and polymerase-mediated primer extension generate an exponential accumulation of a specifc targeted fragment that can be analyzed by a variety of methods. Under these conditions, the first successful genomic PCRs resulted in dramatic amplification and enrichment of the targeted 110 bp fragment of -globin, but only around 1% of the amplified DNA was the target. By allowing primer annealing and extension at elevated temperatures, the use of a thermostable polymerase also greatly increased the specificity of target amplification. Discovery of DNA polymerases that could synthesize a cDNA strand from an RNA template made possible PCR assays for gene expression as well as detection and characteriz...
http://genengnews.com/gen-articles/development-and-evolution-of-pcr/4801/?kwrd=DNA
*  PCR product stays on agarose gel well !! - PCR, RT-PCR and Real-Time PCR
And another "theory" have you tried to clean up your PCR product column or precipitation, not enzymatic, maybe something in your PCR buffer causes "strange" running of your template. In this gel I just ran a random PCR product, probably one of the primer sets that did not work. I do get the correct band when using primers for a different gene amplicon that we use in another project and I was using here as positive control. Anyway, we had a student run a PCR will run gel today, with a plate rather than tubes like I was using, and some of my tubes also. Will know soon if there is something wrong with the tubes, or with me. And another "theory" have you tried to clean up your PCR product column or precipitation, not enzymatic, maybe something in your PCR buffer causes "strange" running of your template. mmf on Jul 1 2009, 04:31 PM said: One of my primer sets I was testing several seem to be working but I still need to optimize to minimize other bands. In this gel I just ran a random PCR product, probably one...
http://protocol-online.org/biology-forums-2/posts/8916.html
*  primers in RT in situ PCR
... rob wesley rwesley at welchlink welch jhu edu mon mar est previous message ligation of alkaline phosphatase treated dna next message primers in rt in situ pcr messages sorted by hi i m trying to work out a method for rt in situ pcr and i m at a conceptual snag in the rt step i want to use the specific downstream primer but i m not sure which one this is is it the one complementary to the end of the mrna can i use one of my pcr primers in this step would an oligo dt primer cause me all kinds of trouble how long should the rt primer be robert wesley johns hopkins university department of neurology previous message ligation of alkaline phosphatase treated dna next message primers in rt in situ pcr messages sorted by more information about the methods mailing list...
http://bio.net/bionet/mm/methods/1995-March/025568.html
*  Best DNA extraction method from BAL-supernatant
best dna extraction method from bal supernatant best dna extraction method from bal supernatant jannik helweg jhelweg at inet uni dk sat jun est previous message free antibody resource next message mushroom hunters messages sorted by looking for recommedations on the best method to extract dna from bronchoalveolar lavage bal supernatant i am doing research on p carinii at my hospital we have a large bank of bal s however as some of the cell pellets already have been used for other purposes in some instances the only material we have left is supernatant originally bal s ml was spun at g for min and supernatant approximately ml saved at c and c i have tried doing simple ethanol precipitation on the supernatants and tried a bio gnome dna isolation kit but by both methods the yield is low and pcr too often negative anybody know better methods for extracting a probably tiny amount of dna from this material thanks jannik helweg larsen dept of infectious diseases hvidovre hospital denmark e mail xxjhelweg at inet un...
http://bio.net/bionet/mm/mycology/1998-June/006751.html
*  BME103:W930 Group1 l2 - OpenWetWare
... BME103:W930 Group1 l2 From OpenWetWare Revision as of 18:49, 25 November 2012 by Brianna S. Ackerman Talk. contribs diff ←Older revision. Current revision diff. Newer revision→ diff. Jump to: navigation, search. BME 103 Fall 2012. Home. People. Lab Write-Up 1. Lab Write-Up 2. Lab Write-Up 3. Course Logistics For Instructors. Photos. Wiki Editing Help. Contents. 1 OUR TEAM 2 LAB 2 WRITE-UP 2.1 Thermal Cycler Engineering 2.2 Protocols 2.3 Research and Development. OUR TEAM. Name: Kevin Chu Experimemtal Protocol Planner. Name: Student Role s. Name: Student Role s. Name: Student Role s. Name: Student Role s. Name: Student Role s. Name: Student Role s. LAB 2 WRITE-UP. Thermal Cycler Engineering. Our re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski. System Design. Key Features. Instructions. Protocols. Materials. Supplied in the Kit. Amount. PCR Machine 1. Fluorimeter 1. Patient’s Template DNA 0.1. Positive Control TBD. Negative Control TBD. 10μM forward prime...
http://openwetware.org/index.php?title=BME103:W930_Group1_l2&oldid=658583
*  cDNA synthesis
... terry tcklau at hotmail com sat feb est previous message mol biol web site next message well genomic dna extraction techniques messages sorted by hello recently i would like to construct a cdna library after inserts were ligated into plasmid it would be transformed into e coli however the transformation efficiency is low then all the clones were proceeded mini prep and analysed by gel electrophoresis from the gel it can be seen that only few clones have inserts and the size is about k but the size of cdna is ranged from to base pair can anyone tell me what is my problem thank you in advance terry previous message mol biol web site next message well genomic dna extraction techniques messages sorted by more information about the methods mailing list...
http://bio.net/bionet/mm/methods/1999-February/073317.html
*  Laboratories
... in join sign in branchburg laboratories bio reference laboratories inc robbins rd branchburg nj honors process containment strawberry hill rd branchburg nj hydromer inc industrial pkwy branchburg nj im clone systems inc chubb way branchburg nj merck co inc river rd branchburg nj life cell corp millennium way branchburg nj im clone systems inc us highway n branchburg nj see more warren laboratories nicox inc independence blvd ste warren nj bio array solutions limited technology dr ste warren nj celgene cellular therapeutics powderhorn dr warren nj see more about us contact us phone book opt out mobile pay bill advertise national telco yellow pages white pages top cities top categories dex media all rights reserved data provided by infogroup acxiom privacy policy and terms of use advertiser login...
http://dexknows.com/local/science_and_engineering/laboratories/geo/co-somerset_county-nj/
*  PCR sequencing
... mika salminen msalminen finnphi at pucc princeton edu tue oct est previous message none next message proper posting of queries and messages messages sorted by as i think this is of general interest i reply to the net doug we at finnphi bitnet do a lot of pcr sequencing with reasonable success here s our protocol in which you may find the answears you need denature pmol of pcr product purified by heating min at c in water snap cool on ice for min add pmol primer add buffer anneal primer for min at c add enzyme label and do the labelling for min at c divide to extension termination reactions and keep at c stop reaction loading buffer there are a few more tricks if your dna is dirty as agarose purified use double amount of enzyme this countereffects the agarose inhibitory effect on enzyme activity if you want to read near the primer add manganese buffer from the newest sequenase kit one tenth of total reaction volume tabor et richardson pnas we do our sequencing using the amersham multiwell system in a mikr...
http://bio.net/bionet/mm/methods/1990-October/002758.html
*  - PCR--RT-PCR-and-Real-Time-PCR
... BioJob BioBlog PubAlert BioTool BioProduct BioForum Protocol Home. Forum Index 1999-2009 Home Forum Index 2009- Home Live Discussion. Top : New Forum Archives 2009- : : PCR--RT-PCR-and-Real-Time-PCR. 331. Scaling up PCR to get more DNA - reply: 5 332. RNA concentration suddenly drops - reply: 3 333. Problem using evagreen evagreen vs sybrgreen - reply: 4 334. PCR to get 10kbp product - reply: 4 335. site-directed mutagenesis primer Tm - reply: 4 336. Single-step nested PCR: how to investigate dynamics. - reply: 2 337. RNA concentration to start RT reaction - reply: 1 338. Random vs oligo primer in preamplification RT - reply: 1 339. Smart Cycler II software and Absolute quantification help - reply: 1 340. cDNA amount in qPCR - reply: 2 341. mRNA integrity for qPCR - reply: 6 342. Strange control contamination in cDNA synthesis - reply: 2 343. how does polymerase stop at the required length - reply: 1 344. Perfect dilution curve, but double peak melting curve on SYBR qPCR - reply: 7 345. Real-time efficie...
http://protocol-online.org/biology-forums-2/PCR--RT-PCR-and-Real-Time-PCR/more11.html
*  PCR-sequencing on colonies
pcr sequencing on colonies pcr sequencing on colonies ajwatson ajwatson at romeo caltech edu thu mar est previous message e mail address next message dopamine receptor ihc messages sorted by in article cnjbn w at biobase aau dk kjaer at biobase aau dk svend kjaer writes i would like to hear from anybody who has succesfully done pcr sequencing directly on bacteriacolonies grown on plates if anyone could advice me some literature or practical approaches i would be very thankfull i do this routinely simply touch a toohpick to the colony resuspend it ul of dh o boil it for min microfuge out the debris and use about ul or so in a ul reaction use the sequencing primers that flank the insert pcr amplify a control plasmid no insert as well one of my labmates claims to done it simply by taking part of the resusupended plasmid no boiling but i don t know about this first hand cheers john previous message e mail address next message dopamine receptor ihc messages sorted by more information about the methods mailing list...
http://bio.net/bionet/mm/methods/1994-March/012941.html
*  PCR anomaly
... john r mcquiston zje at cdc gov wed oct est previous message pcr anomaly next message pcr anomaly messages sorted by it could also be primer dimer formation which raising the annealing temp should take care of bp is a little high for that but it s a possibility john in article bf f at le ac uk afs at le ac uk says mark edward bowen wrote my pcr reactions have suddenly become dominated by two well defined and reproduceable small mw bp products it happens with different buffers enzymes primers and templates the products always appear to be the same size i can anneal at temperatures up to c and only lose my desired product i ve tried hot start touch down mg and temperature titrations as well as varying template and primer concentration i m at a loss any ideas i use a stratagene robocycler do the extra bands occur in the pcr negative control and rt negative control if applicable have you done the following controls no template no primers no template or primers it would also be helpful to give an idea of what...
http://bio.net/bionet/mm/methods/1999-October/078660.html
*  competitive PCR
... mark pavlick mpavlick at popserver nih gov wed jun est previous message prep of m rf dna next message nitrosoguanidine messages sorted by i m beginning work with a variety of competitive pcr and would appreciate advice from anyone who has used this technique e mail mpavlick at popserver nih gov previous message prep of m rf dna next message nitrosoguanidine messages sorted by more information about the methods mailing list...
http://bio.net/bionet/mm/methods/1994-June/015527.html
*  Haynes:Protocols - OpenWetWare
... ==Cell Culture: Bacteria==. ==Cell Culture: Bacteria==. ==Cell Culture: Mammalian==. ==Cell Culture: Mammalian==. * ] - cell line-specific formulas. * ] - cell line-specific formulas. ==Real Time Quantitative PCR==. ==Real Time Quantitative PCR==. + == RNA cDNA protocols==. * : buffer formulas; specific information on commonly used reagents 'e.g.', LB broth, ampicillin, restriction enzymes, etc. * : buffer formulas; specific information on commonly used reagents 'e.g.', LB broth, ampicillin, restriction enzymes, etc. - 'Bromo-Blue/X-cyanol Loading Buffer, 10X ' {{hide. + 'Bromo-Blue/X-cyanol Loading Buffer, 20X ' {{hide. Volume: 20 mL. Volume: 20 mL. - Bring up to total volume with dH sub 2 /sub O. Add in glycerol 6 mL at a time and mix by pipetting up and down several times it is very viscous. Bring up to total volume with dH sub 2 /sub O. Add 100x protease inhibitor cocktail* 1:100 immediately before use. Bring up to total volume with dH sub 2 /sub O. Add 100x protease inhibitor cocktail* 1:100 immedia...
http://openwetware.org/index.php?title=Haynes:Protocols&diff=666371&oldid=646964
*  Problem using pfuturbo for PCR
... Alan Smith smitam01 at HOLMES.IPFW.EDU. Wed Jun 16 16:54:40 EST 1999. Previous message: Glycerol PAGE toughening Next message: Problem using pfuturbo for PCR Messages sorted by:. Hello Over the past two weeks we have been trying to use pfuturbo DNA polymerase to amplify a 2.3 Kb amplicon. When using pfuturboDNA polymerase we get an 800 bp amplicon, but when using taqDNA polymerase we get the proper 2.3 KB amplicon. My first hunch was that the pfuturbo we were using was contaminated with another primer causing the problem in amplification. However, if there was contamination I would expect two or more bands on my gels corresponding to one primer complementing another the contaminate and another band corresponding to the primer pair producing the proper amplicon, this was not the case. The product we get with pfuturbo is one very specific looking band on an agarose gel at 800bp and when we use taq we get one specific band of the expected size 2.3kb and does prove to be the correct product when it is digest...
http://bio.net/bionet/mm/methods/1999-June/076147.html
*  Uni-directional deletions
uni directional deletions uni directional deletions mary p remington mremingt at umabnet ab umd edu mon jun est previous message thermal cyclers for less than next message expressing truncated proteins messages sorted by i ve had great success with stratagene s exo mung bean deletion kit mary previous message thermal cyclers for less than next message expressing truncated proteins messages sorted by more information about the methods mailing list...
http://bio.net/bionet/mm/methods/1996-June/045257.html
*  Troubleshooting reverse transcriptase-PCR - PCR, RT-PCR and Real-Time PCR - BioForum
... Remember me This is not recommended for shared computers Sign in anonymously Don't add me to the active users list Privacy Policy. Sign In. Forums. Forums. BioForum. PCR, RT-PCR and Real-Time PCR. Troubleshooting reverse transcriptase-PCR Started by. I have no bands in the gel after doing my PCR. I have tried RT with random hexamers as well as oligo dT primers. Till now I am sure that my starting material total RNA is fine...I don't know if the problem is in the RT reaction or in the PCR process...I always do two step RT-PCR...First I do RT reaction...denaturing of 4 ul RNA with 1ul dT primer at 70C for 5 min..then quickly on ice..then making the RT mix using 1 ul RT enzyme and the buffer and nuclease free water in a total volume of 20 ul...the RT reaction lasts for 80 min. After that I proceed with PCR using random hexamers along with oligo dT primer...with no amplification results at all for two months now... Sometimes I used to run a volume from the product of RT reaction on 1% agarose gel ...ther...
http://protocol-online.org/forums/topic/18263-troubleshooting-reverse-transcriptase-pcr/
*  Ligation of PCR products
... Andreas Pahl macbeth at cs.tu-berlin.de. Thu Jul 25 06:18:16 EST 1991. Previous message: Ligation of PCR products Next message: pro vs eu Messages sorted by:. In article 1991Jul19.131027.10619 at nrcnet0.nrc.ca, num208jn at MBDS.NRC.CA John Nash writes: In article 9107182217.AA23884 at genbank.bio.net, ST403161 at BROWNVM.BROWN.EDU Michael Newstein writes: I have read that it is exceedingly difficult to blunt-end ligate products from PCR without cleaving first in internal restriction sites. I am attempting to clone the product of a PCR reaction into a blunt end cutter site eg SMA 1. in pBluescript. Does anyone have suggestions. ... By the way, when I cloned my 650 bp PCR product, the positive clones were all light blue.... I was cursing because the transformation plates had few white colonies. Hope this helps. Did you isolate the DNA from all the colonies?. How did you confirm the positive clones?. Bye, Andreas --...
http://bio.net/bionet/mm/methods/1991-July/002871.html
*  digesting pcr products
... Paul Rudnick rudnick at flash.net. Tue May 20 01:34:07 EST 1997. Previous message: Free: Program DYNAFIT correction. Next message: digesting pcr products Messages sorted by:. Hello- I have been repeatedly trying to clone a pcr product generated from primers containg engineered restriction sites. I am aware that some enzymes require more than a few bases downstream to properly cut, however I did check a chart for cutting the ends of fragments and these enzymes should have sufficient room. The product was generated with pfu and the enzymes are HindIII and HincII. Any ideas why the product won't clone. Thank in advance. Paul. Previous message: Free: Program DYNAFIT correction. Next message: digesting pcr products Messages sorted by:. More information about the Methods mailing list....
http://bio.net/bionet/mm/methods/1997-May/057616.html
*  restrict. digestion of PCR products
... sl3wx at cc.usu.edu sl3wx at cc.usu.edu. Mon Aug 28 17:27:20 EST 1995. Previous message: Deng/Nickoloff mutagenesis Next message: Western Blot Messages sorted by:. This may help those having problems restriction-digesting their PCR products. I had a similar problem wherein the enzymes would'nt cut at their sites which had been tailored into the PCR primers. I was recommended pOCUS T-Vector from Novagen and it worked just fine. It is a linearized form of plasmid which has a 3'dT at each end facilitating ligation with any DNA amplified with a poymerase that leaves single 3 dA overhangs e.g. Taq poymerase. The insert is then cut out of pOCUS. Hope this helps. Previous message: Deng/Nickoloff mutagenesis Next message: Western Blot Messages sorted by:. More information about the Methods mailing list....
http://bio.net/bionet/mm/methods/1995-August/032788.html
*  Overlay assay
... an overlay assay is a biological technique used to find protein s that bind to a protein of interest it begins with proteins that are dispersed but fixed e g a tissue slice or run on a membrane by gel electrophoresis and then incubated in the labelled protein of interest the label will indicate the location of proteins that bind to the protein of interest category laboratory techniques...
https://en.wikipedia.org/wiki/Overlay_assay
*  96-well PCR cleanup?
well pcr cleanup well pcr cleanup larry larry fri apr est previous message abi xl upgrade next message skipped region messages sorted by hello fellow sequencers i am beginning a project in which i will be sequencing a few thousand pcr products using an dna sequencer because of the number of pcr products i would like to use dye terminator chemistries and a well format i am a little concerned about obtaining a clean no primers salts etc pcr product so that i can use the dye terminator chemistries without getting interference from the pcr primers and rxn mix i was wondering if anyone has some good ideas on how to clean the pcrs in a well format or should i resort to dye primer chemistries thank you in advance larry grocki lexicon genetics lgrocki at lexgen com previous message abi xl upgrade next message skipped region messages sorted by more information about the autoseq mailing list...
http://bio.net/bionet/mm/autoseq/1997-April/000540.html
*  Genomic DNA Isolation - NucleoSpin Buffers
genomic dna isolation nucleospin buffers...
http://clontech.com/US/Products/Nucleic_Acid_Purification/Accessories/Buffers_and_Enzymes/Genomic_DNA?sitex=10020:22372:US&PROD=-EAXiC2NvpzLFKk6VqP56BW0:S&PROD_pses=ZGB661E45C2AC1C3B257A19DC90E46AA79FD591468F53379C47FE109276732526C238C526166018BD1414D25AE19FEC0382D26E0F3C3155298
*  Genomic DNA Isolation - NucleoSpin Buffers
genomic dna isolation nucleospin buffers...
http://clontech.com/US/Products/Nucleic_Acid_Purification/Accessories/Buffers_and_Enzymes/Genomic_DNA?sitex=10020:22372:US&PROD=2sCUPejCJVMrHSfsBFNLqSsz:S&PROD_pses=ZGACB597827C90FDCF1274FC92274E7A7CB858CDC402135B82D221014E781E935AE5B817CB8915649D03B3F7BE6352AFA19D17596C81525D96
*  Genomic DNA Isolation - NucleoSpin Buffers
genomic dna isolation nucleospin buffers...
http://clontech.com/US/Products/Nucleic_Acid_Purification/Accessories/Buffers_and_Enzymes/Genomic_DNA?sitex=10020:22372:US&PROD=3_U0GeHoSVtXPWe_JeyTBun5:S&PROD_pses=ZGEEEAC3954E3A545D4320D7FA8298629FFEB4F26E82D01B1200AA6CA0D1F907BF5DB259D091B2F27ABB13F3261AE7160B6F56E4DFF5D3E199
*  Genomic DNA Isolation - NucleoSpin Buffers
. Genomic DNA Isolation - NucleoSpin Buffers....
http://clontech.com/US/Products/Nucleic_Acid_Purification/Accessories/Buffers_and_Enzymes/Genomic_DNA?sitex=10020:22372:US&PROD=52oqQd_xX6RuFbRpILH_ck8R:S&PROD_pses=ZGC226C96DF66589E661C8009003A2638A395265AB05DD5CFD5F1A9D4733C49459EB2D079D01AF80F0C0DB08D4E54C283E07C39A2D8AA13311
*  Genomic DNA Isolation - NucleoSpin Buffers
genomic dna isolation nucleospin buffers...
http://clontech.com/US/Products/Nucleic_Acid_Purification/Accessories/Buffers_and_Enzymes/Genomic_DNA?sitex=10020:22372:US&PROD=F3jkFZmIwsaR2FkLL0L-W4sI:S&PROD_pses=ZGCB1B5EEE1D29970E0B659A5A5FE4DB9A68EA8F007398E779A4D18FA82A98AC1CC7334EF6C23C4B2B17BF8A850D7E84A09C8B82E1CEC2B593
*  Genomic DNA Isolation - NucleoSpin Buffers
. Genomic DNA Isolation - NucleoSpin Buffers....
http://clontech.com/US/Products/Nucleic_Acid_Purification/Accessories/Buffers_and_Enzymes/Genomic_DNA?sitex=10020:22372:US&PROD=Fl5-JyyXIVxAEVdJjJKo9zYw:S&PROD_pses=ZG4A2C5810A6D028BF7F3D6C794F1045491C5386A60836E0E14FDD63A50014651D11A1B1B6A677EE445AA386930381DD63E374B4A30509BED8
*  Genomic DNA Isolation - NucleoSpin Buffers
genomic dna isolation nucleospin buffers...
http://clontech.com/US/Products/Nucleic_Acid_Purification/Accessories/Buffers_and_Enzymes/Genomic_DNA?sitex=10020:22372:US&PROD=HXPH761M1kkXbNqP3JAXnZSy:S&PROD_pses=ZG34B9C8EDB9DE0591E61CB1FCB93ADA3BD9972500301A677FA4E2776A43BC3923E510BFE040E1471D07342DAFF35C4B657C38D3306B9111D0
*  Genomic DNA Isolation - NucleoSpin Buffers
. Genomic DNA Isolation - NucleoSpin Buffers....
http://clontech.com/US/Products/Nucleic_Acid_Purification/Accessories/Buffers_and_Enzymes/Genomic_DNA?sitex=10020:22372:US&PROD=Mw0WjSuF-xr39SpFHRY9TS8l:S&PROD_pses=ZGD07B7FBAB1E10573304EC7F1C243C18441A0A543DD4860AA5C5AA413E6A16A2123B2C09D65BCC68465BB26241374BA5232D9CBE062FCA0CA
*  Genomic DNA Isolation - NucleoSpin Buffers
genomic dna isolation nucleospin buffers...
http://clontech.com/US/Products/Nucleic_Acid_Purification/Accessories/Buffers_and_Enzymes/Genomic_DNA?sitex=10020:22372:US&PROD=Nn196UxyizVNCcLAPHeFYass:S&PROD_pses=ZGA1F9002D32F1A4475CD106DA6ECD9B040B03A6CB4D4FCF4AACE666E6A727D46E1454562E4EED05D52FDBA1BABD8CFA62F31D25BDC0D33E05
*  Genomic DNA Isolation - NucleoSpin Buffers
genomic dna isolation nucleospin buffers...
http://clontech.com/US/Products/Nucleic_Acid_Purification/Accessories/Buffers_and_Enzymes/Genomic_DNA?sitex=10020:22372:US&PROD=RJgOgdfjqS14SJ6rV8yB6ZIS:S&PROD_pses=ZGAD56D2528C32E474C239D70FF6282D538C4CDA0F94DB53A48EC168B44C8143A5990B28A2BD9080630F5BA743622BBA2B097947B505A31EE1
*  Genomic DNA Isolation - NucleoSpin Buffers
genomic dna isolation nucleospin buffers...
http://clontech.com/US/Products/Nucleic_Acid_Purification/Accessories/Buffers_and_Enzymes/Genomic_DNA?sitex=10020:22372:US&PROD=iRpj4Tzzg8RVbHw1zcVY8up-:S&PROD_pses=ZGB0B8BB64D89E3EE14767A6A2F11BB22E164BC1E07D1AC2F4736B436F77BD0916D0A3283E4E4E5388D07FA514A8B2D31FE417D2C46AB068BF
*  Genomic DNA Isolation - NucleoSpin Buffers
genomic dna isolation nucleospin buffers...
http://clontech.com/US/Products/Nucleic_Acid_Purification/Accessories/Buffers_and_Enzymes/Genomic_DNA?sitex=10020:22372:US&PROD=j_IA4Ue7TTyky5cjiqBJvPCH:S&PROD_pses=ZG24F84A5D161E804F03EE6E2A229E53D8D78C6E522F46FBEECEAF54ECCBC07C4D8474CBC4F5CB3FB23DA2551633CCEDAF23557B37D30EC6A5
*  Genomic DNA Isolation - NucleoSpin Buffers
genomic dna isolation nucleospin buffers...
http://clontech.com/US/Products/Nucleic_Acid_Purification/Accessories/Buffers_and_Enzymes/Genomic_DNA?sitex=10020:22372:US&PROD=lHU7zUGn2ELwYgfSfzH95Luy:S&PROD_pses=ZG015F4F47ABAC37133D8B4DE79E52ECB53778FB44EE4C0DA468E2874BD1D015DA2BAED6259553043FE91E599224770F8961B46A476F451174
*  Genomic DNA Isolation - NucleoSpin Buffers
. Genomic DNA Isolation - NucleoSpin Buffers....
http://clontech.com/US/Products/Nucleic_Acid_Purification/Accessories/Buffers_and_Enzymes/Genomic_DNA?sitex=10020:22372:US&PROD=nesMm_4LrD1cLmaZQke-XJ4t:S&PROD_pses=ZG257EF831C6F1B7164FDF03FE7BD4809738F9BBD1C975C24091FF3620970595E7DA08C0010DFEF50D543147D3822EB8BA96E477D36034B13F
*  Genomic DNA Isolation - NucleoSpin Buffers
genomic dna isolation nucleospin buffers...
http://clontech.com/US/Products/Nucleic_Acid_Purification/Accessories/Buffers_and_Enzymes/Genomic_DNA?sitex=10020:22372:US&PROD=z5-8UCGpay0hMhFnRs-IZXTR:S&PROD_pses=ZGBED850B16DF40B589E69321AED9185C3C26A33194F86808763D88C989E010A005F5C62A76464B07A497A332C0E75D1965FDBECFB5F92FBC2
*  Category:Cell imaging
category cell imaging category cell imaging cell imaging techniques are techniques of microscopy used in cell biology category microscopy category cell biology category biotechnology category biological techniques and tools...
https://en.wikipedia.org/wiki/Category:Cell_imaging
*  SIGMAR1 Gene - GeneCards | SGMR1 Protein | SGMR1 Antibody
Research Products for SIGMAR1 Gene Antibodies. Antibodies. Proteins Enzymes Antibodies Assays Kits. Proteins Antibodies Assays Genes shRNA Primers CRISPR. OriGene Purified Proteins for SIGMAR1 NM 005866 Search Origene for MassSpec and Protein Over-expression Lysates for SIGMAR1. miRNA Products QIAGEN qRT-PCR Assays for microRNAs that regulate SIGMAR1 hsa-miR-3919. Clone Products OriGene clones in human, mouse for SIGMAR1 OriGene ORF clones in human, mouse, rat for SIGMAR1. Cell Line Products GenScript Custom overexpressing Cell Line Services for SIGMAR1. miRNA Products QIAGEN qRT-PCR Assays for microRNAs that regulate SIGMAR1 hsa-miR-3919. Clone Products OriGene clones in human, mouse for SIGMAR1 OriGene ORF clones in human, mouse, rat for SIGMAR1. Browse Small Molecules at EMD Millipore EMD Millipore Mono- and Polyclonal Antibodies for SIGMAR1 EMD Millipore Purified and/or Recombinant SIGMAR1 Protein. OriGene Purified Proteins for SIGMAR1 NM 005866 Search Origene for MassSpec and Protein Over-expression Lysa...
http://genecards.org/cgi-bin/carddisp.pl?gene=SIGMAR1
*  MMP15 Gene - GeneCards | MMP15 Protein | MMP15 Antibody
Research Products for MMP15 Gene Antibodies. miRNA. Antibodies. Proteins Enzymes Antibodies Assays Kits. Proteins Antibodies Assays Genes shRNA Primers CRISPR. MT-MMP 2 3. MT2-MMP 3. MT2MMP 3. Regulatory Element Products SwitchGear MMP15 promoter sequence S709006 Browse SwitchGear Promoter luciferase reporter plasmids. miRNA Products QIAGEN qRT-PCR Assays for microRNAs that regulate MMP15 hsa-miR-4300. Clone Products OriGene clones in human, mouse for MMP15 OriGene ORF clones in human, mouse, rat for MMP15. Cell Line Products GenScript Custom overexpressing Cell Line Services for MMP15. miRNA Products QIAGEN qRT-PCR Assays for microRNAs that regulate MMP15 hsa-miR-4300. Clone Products OriGene clones in human, mouse for MMP15 OriGene ORF clones in human, mouse, rat for MMP15. Assignment of the human genes for membrane-type-1, -2, and -3 matrix metalloproteinases MMP14, MMP15, and MMP16 to 14q12.2, 16q12.2-q21, and 8q21, respectively, by in situ hybridization. Browse Small Molecules at EMD Millipore EMD Millipo...
http://genecards.org/cgi-bin/carddisp.pl?id_type=entrezgene&id=4324
*  GRIN2D Gene - GeneCards | NMDE4 Protein | NMDE4 Antibody
GRIN2D. Research Products for GRIN2D Gene Antibodies. miRNA. Antibodies. Proteins Enzymes Antibodies Assays Kits. Proteins Antibodies Assays Genes shRNA Primers CRISPR. Regulatory Element Products SwitchGear GRIN2D promoter sequence S709203 Browse SwitchGear Promoter luciferase reporter plasmids. Search Origene for Purified Proteins, MassSpec and Protein Over-expression Lysates for GRIN2D. miRNA Products QIAGEN qRT-PCR Assays for microRNAs that regulate GRIN2D hsa-miR-1539. Clone Products OriGene clones in human, mouse for GRIN2D OriGene ORF clones in human, mouse, rat for GRIN2D. Cell Line Products GenScript Custom overexpressing Cell Line Services for GRIN2D. miRNA Products QIAGEN qRT-PCR Assays for microRNAs that regulate GRIN2D hsa-miR-1539. Clone Products OriGene clones in human, mouse for GRIN2D OriGene ORF clones in human, mouse, rat for GRIN2D. Browse Small Molecules at EMD Millipore EMD Millipore Mono- and Polyclonal Antibodies for GRIN2D EMD Millipore Purified and/or Recombinant GRIN2D Protein. Cust...
http://genecards.org/cgi-bin/carddisp.pl?gene=GRIN2D&ortholog_desc=all
*  TAP2 Gene - GeneCards | TAP2 Protein | TAP2 Antibody
TAP2. Research Products for TAP2 Gene Antibodies. Antibodies. Proteins Enzymes Antibodies Assays Kits. Proteins Antibodies Assays Genes shRNA Primers CRISPR. Genes Peptides Proteins CRISPR. ABCB3 3. Regulatory Element Products SwitchGear TAP2 promoter sequence S715029 Browse SwitchGear Promoter luciferase reporter plasmids. Antibody Products Browse EMD Millipore's Extensive Line of Mono- and Polyclonal Antibodies. Protein Products Browse Purified and Recombinant Proteins at EMD Millipore. Search Origene for Purified Proteins, MassSpec and Protein Over-expression Lysates for TAP2. miRNA Products QIAGEN qRT-PCR Assays for microRNAs that regulate TAP2 hsa-miR-330-3p. Inhibitory RNA Products Origene siRNA, shRNA, and RNAi products in human, mouse, rat for TAP2. Clone Products OriGene clones in human, mouse for TAP2 OriGene ORF clones in human, mouse, rat for TAP2. Cell Line Products GenScript Custom overexpressing Cell Line Services for TAP2. miRNA Products QIAGEN qRT-PCR Assays for microRNAs that regulate TA...
http://genecards.org/cgi-bin/carddisp.pl?gene=TAP2&go_proc=20&rf=/home/genecards/current/website/carddisp.pl
*  TAP2 Gene - GeneCards | TAP2 Protein | TAP2 Antibody
TAP2. Research Products for TAP2 Gene Antibodies. Antibodies. Proteins Enzymes Antibodies Assays Kits. Proteins Antibodies Assays Genes shRNA Primers CRISPR. Genes Peptides Proteins CRISPR. ABCB3 3. Regulatory Element Products SwitchGear TAP2 promoter sequence S715029 Browse SwitchGear Promoter luciferase reporter plasmids. Antibody Products Browse EMD Millipore's Extensive Line of Mono- and Polyclonal Antibodies. Protein Products Browse Purified and Recombinant Proteins at EMD Millipore. Search Origene for Purified Proteins, MassSpec and Protein Over-expression Lysates for TAP2. miRNA Products QIAGEN qRT-PCR Assays for microRNAs that regulate TAP2 hsa-miR-330-3p. Inhibitory RNA Products Origene siRNA, shRNA, and RNAi products in human, mouse, rat for TAP2. Clone Products OriGene clones in human, mouse for TAP2 OriGene ORF clones in human, mouse, rat for TAP2. Cell Line Products GenScript Custom overexpressing Cell Line Services for TAP2. miRNA Products QIAGEN qRT-PCR Assays for microRNAs that regulate TA...
http://genecards.org/cgi-bin/carddisp.pl?gene=TAP2&origene_3utr_trans=2&rf=/home/genecards/current/website/carddisp.pl
*  TAP2 Gene - GeneCards | TAP2 Protein | TAP2 Antibody
TAP2. Research Products for TAP2 Gene Antibodies. Antibodies. Proteins Enzymes Antibodies Assays Kits. Proteins Antibodies Assays Genes shRNA Primers CRISPR. Genes Peptides Proteins CRISPR. ABCB3 3. Regulatory Element Products SwitchGear TAP2 promoter sequence S715029 Browse SwitchGear Promoter luciferase reporter plasmids. Antibody Products Browse EMD Millipore's Extensive Line of Mono- and Polyclonal Antibodies. Protein Products Browse Purified and Recombinant Proteins at EMD Millipore. Search Origene for Purified Proteins, MassSpec and Protein Over-expression Lysates for TAP2. miRNA Products QIAGEN qRT-PCR Assays for microRNAs that regulate TAP2 hsa-miR-330-3p. Inhibitory RNA Products Origene siRNA, shRNA, and RNAi products in human, mouse, rat for TAP2. Clone Products OriGene clones in human, mouse for TAP2 OriGene ORF clones in human, mouse, rat for TAP2. Cell Line Products GenScript Custom overexpressing Cell Line Services for TAP2. miRNA Products QIAGEN qRT-PCR Assays for microRNAs that regulate TA...
http://genecards.org/cgi-bin/carddisp.pl?gene=TAP2&ortholog=all&rf=/home/genecards/current/website/carddisp.pl
*  HOXD3 Gene - GeneCards | HXD3 Protein | HXD3 Antibody
Research Products for HOXD3 Gene Antibodies. Proteins Enzymes Antibodies Assays Kits. Proteins Antibodies Assays Genes shRNA Primers CRISPR. Antibody Products Browse EMD Millipore's Extensive Line of Mono- and Polyclonal Antibodies. Protein Products Browse Purified and Recombinant Proteins at EMD Millipore. miRNA Products QIAGEN qRT-PCR Assays for microRNAs that regulate HOXD3 hsa-miR-3671. Inhibitory RNA Products Origene siRNA, shRNA, and RNAi products in human, mouse, rat for HOXD3. Clone Products OriGene clones in human, mouse for HOXD3 OriGene ORF clones in human, mouse, rat for HOXD3. Cell Line Products GenScript Custom overexpressing Cell Line Services for HOXD3. miRNA Products QIAGEN qRT-PCR Assays for microRNAs that regulate HOXD3 hsa-miR-3671. Inhibitory RNA Products Origene siRNA, shRNA, and RNAi products in human, mouse, rat for HOXD3. Clone Products OriGene clones in human, mouse for HOXD3 OriGene ORF clones in human, mouse, rat for HOXD3. Browse EMD Millipore's Extensive Line of Mono- and...
http://genecards.org/cgi-bin/carddisp.pl?gene=HOXD3
*  FOXC1 Gene - GeneCards | FOXC1 Protein | FOXC1 Antibody
Research Products for FOXC1 Gene Antibodies. miRNA. Antibodies. Proteins Enzymes Antibodies Assays Kits. Proteins Antibodies Assays Genes shRNA Primers CRISPR. Regulatory Element Products SwitchGear FOXC1 promoter sequence S710379 Browse SwitchGear Promoter luciferase reporter plasmids. miRNA Products QIAGEN qRT-PCR Assays for microRNAs that regulate FOXC1 hsa-miR-133a. Clone Products OriGene clones in human, mouse for FOXC1 OriGene ORF clones in human, mouse, rat for FOXC1. Cell Line Products GenScript Custom overexpressing Cell Line Services for FOXC1. miRNA Products QIAGEN qRT-PCR Assays for microRNAs that regulate FOXC1 hsa-miR-133a. Clone Products OriGene clones in human, mouse for FOXC1 OriGene ORF clones in human, mouse, rat for FOXC1. Browse Small Molecules at EMD Millipore EMD Millipore Mono- and Polyclonal Antibodies for FOXC1 EMD Millipore Purified and/or Recombinant FOXC1 Protein EMD Millipore Kits and Assays for FOXC1. Custom Assay Services Search Origene for Purified Proteins, MassSpec and Prote...
http://genecards.org/cgi-bin/carddisp.pl?gene=FOXC1
*  DLX5 Gene - GeneCards | DLX5 Protein | DLX5 Antibody
Research Products for DLX5 Gene Antibodies. Proteins Enzymes Antibodies Assays Kits. Proteins Antibodies Assays Genes shRNA Primers CRISPR. miRNA Products QIAGEN qRT-PCR Assays for microRNAs that regulate DLX5 hsa-miR-466. Inhibitory RNA Products Origene siRNA, shRNA, and RNAi products in human, mouse, rat for DLX5. Clone Products OriGene clones in human, mouse for DLX5 OriGene ORF clones in human, mouse, rat for DLX5. Cell Line Products GenScript Custom overexpressing Cell Line Services for DLX5. miRNA Products QIAGEN qRT-PCR Assays for microRNAs that regulate DLX5 hsa-miR-466. Inhibitory RNA Products Origene siRNA, shRNA, and RNAi products in human, mouse, rat for DLX5. Clone Products OriGene clones in human, mouse for DLX5 OriGene ORF clones in human, mouse, rat for DLX5. mRNA expression in embryonic tissues and stem cells from LifeMap Discovery. Browse Small Molecules at EMD Millipore EMD Millipore Mono- and Polyclonal Antibodies for DLX5 EMD Millipore Purified and/or Recombinant DLX5 Protein. Custom Assa...
http://genecards.org/cgi-bin/carddisp.pl?gene=DLX5&sabio_mir_transcripts=13
*  GFI1 Gene - GeneCards | GFI1 Protein | GFI1 Antibody
Research Products for GFI1 Gene Antibodies. Proteins. Antibodies. Proteins Enzymes Antibodies Assays Kits. Proteins Antibodies Assays Genes shRNA Primers CRISPR. Genes Peptides Proteins CRISPR. ZNF163 3. Previous HGNC Symbols for GFI1 Gene ZNF163. NM 001127216 Search Origene for MassSpec and Protein Over-expression Lysates for GFI1. miRNA Products QIAGEN qRT-PCR Assays for microRNAs that regulate GFI1 hsa-miR-4255. Inhibitory RNA Products Origene siRNA, shRNA, and RNAi products in human, mouse, rat for GFI1. Clone Products OriGene clones in human, mouse for GFI1 OriGene ORF clones in human, mouse, rat for GFI1. Cell Line Products GenScript Custom overexpressing Cell Line Services for GFI1. miRNA Products QIAGEN qRT-PCR Assays for microRNAs that regulate GFI1 hsa-miR-4255. Inhibitory RNA Products Origene siRNA, shRNA, and RNAi products in human, mouse, rat for GFI1. Clone Products OriGene clones in human, mouse for GFI1 OriGene ORF clones in human, mouse, rat for GFI1. Selected SIMAP similar genes for GFI1 Gen...
http://genecards.org/cgi-bin/carddisp.pl?id_type=entrezgene&id=2672
*  SLC16A1 Gene - GeneCards | MOT1 Protein | MOT1 Antibody
Research Products for SLC16A1 Gene Antibodies. miRNA. Proteins Enzymes Antibodies Assays Kits. Proteins Antibodies Assays Genes shRNA Primers CRISPR. Regulatory Element Products SwitchGear SLC16A1 promoter sequence S709315 Browse SwitchGear Promoter luciferase reporter plasmids. OriGene Purified Proteins for SLC16A1 NM 003051 Search Origene for MassSpec and Protein Over-expression Lysates for SLC16A1. miRNA Products QIAGEN qRT-PCR Assays for microRNAs that regulate SLC16A1 hsa-miR-3148. Clone Products OriGene clones in human, mouse for SLC16A1 OriGene ORF clones in human, mouse, rat for SLC16A1. Cell Line Products GenScript Custom overexpressing Cell Line Services for SLC16A1. miRNA Products QIAGEN qRT-PCR Assays for microRNAs that regulate SLC16A1 hsa-miR-3148. Clone Products OriGene clones in human, mouse for SLC16A1 OriGene ORF clones in human, mouse, rat for SLC16A1. Browse Small Molecules at EMD Millipore EMD Millipore Mono- and Polyclonal Antibodies for SLC16A1 EMD Millipore Purified and/or Recombinant ...
http://genecards.org/cgi-bin/carddisp.pl?gene=SLC16A1
*  BCL2 Gene - GeneCards | BCL2 Protein | BCL2 Antibody
miRNA. miRNA Products QIAGEN qRT-PCR Assays for microRNAs that regulate BCL2 hsa-miR-448. miRNA Products QIAGEN qRT-PCR Assays for microRNAs that regulate BCL2 hsa-miR-448. Pathway & Disease-focused RT² Profiler PCR Arrays Apoptosis in human, mouse, rat Apoptosis 384HT in human, mouse, rat Atherosclerosis in human, mouse, rat Autophagy in human, mouse, rat Breast Cancer in human, mouse, rat Cancer Drug Resistance in human, mouse, rat Cancer Drug Targets in human, mouse, rat Cell Cycle in human, mouse, rat Cell Death PathwayFinder in human, mouse, rat EGF / PDGF Signaling Pathway in human, mouse, rat Endothelial Cell Biology in human, mouse, rat Fibrosis in human, mouse, rat G-Protein-Coupled Receptor Signaling PathwayFinder in human, mouse, rat HIV Host Response in human, mouse, rat Hedgehog Signaling Pathway in human, mouse, rat Inflammasomes in human, mouse, rat Leukemia in human, mouse, rat Liver Cancer in human, mouse, rat Lung Cancer in human, mouse, rat Lymphoma in human, mouse, rat Mitochondria in huma...
http://genecards.org/cgi-bin/carddisp.pl?gene=BCL2&phen=21
*  SLC34A2 Gene - GeneCards | NPT2B Protein | NPT2B Antibody
Research Products for SLC34A2 Gene Antibodies. Regulatory Element Products SwitchGear SLC34A2 promoter sequence S707576 Browse SwitchGear Promoter luciferase reporter plasmids. Antibody Products Browse EMD Millipore's Extensive Line of Mono- and Polyclonal Antibodies. Protein Products Browse Purified and Recombinant Proteins at EMD Millipore. OriGene Purified Proteins for SLC34A2 NM 006424 Search Origene for MassSpec and Protein Over-expression Lysates for SLC34A2. miRNA Products QIAGEN qRT-PCR Assays for microRNAs that regulate SLC34A2 hsa-let-7a-2*. Clone Products OriGene clones in human, mouse for SLC34A2 OriGene ORF clones in human, mouse, rat for SLC34A2. Cell Line Products GenScript Custom overexpressing Cell Line Services for SLC34A2. miRNA Products QIAGEN qRT-PCR Assays for microRNAs that regulate SLC34A2 hsa-let-7a-2*. Clone Products OriGene clones in human, mouse for SLC34A2 OriGene ORF clones in human, mouse, rat for SLC34A2. Selected SIMAP similar genes for SLC34A2 Gene using alignment to 3 pr...
http://genecards.org/cgi-bin/carddisp.pl?gene=SLC34A2
*  BCL3 Gene - GeneCards | BCL3 Protein | BCL3 Antibody
Research Products for BCL3 Gene Antibodies. miRNA. Proteins Enzymes Antibodies Assays Kits. Proteins Antibodies Assays Genes shRNA Primers CRISPR. OriGene Purified Proteins for BCL3 NM 005178 Search Origene for MassSpec and Protein Over-expression Lysates for BCL3. miRNA Products QIAGEN qRT-PCR Assays for microRNAs that regulate BCL3 hsa-miR-324-3p. Inhibitory RNA Products Origene siRNA, shRNA, and RNAi products in human, mouse, rat for BCL3. Clone Products OriGene clones in human, mouse for BCL3 OriGene ORF clones in human, mouse, rat for BCL3. Cell Line Products GenScript Custom overexpressing Cell Line Services for BCL3. miRNA Products QIAGEN qRT-PCR Assays for microRNAs that regulate BCL3 hsa-miR-324-3p. Inhibitory RNA Products Origene siRNA, shRNA, and RNAi products in human, mouse, rat for BCL3. Clone Products OriGene clones in human, mouse for BCL3 OriGene ORF clones in human, mouse, rat for BCL3. Browse Small Molecules at EMD Millipore EMD Millipore Mono- and Polyclonal Antibodies for BCL3 EMD Millipo...
http://genecards.org/cgi-bin/carddisp.pl?gene=BCL3
*  IL2RB Gene - GeneCards | IL2RB Protein | IL2RB Antibody
Research Products for IL2RB Gene Antibodies. Antibody Products Browse EMD Millipore's Extensive Line of Mono- and Polyclonal Antibodies. Protein Products Browse Purified and Recombinant Proteins at EMD Millipore. miRNA Products QIAGEN qRT-PCR Assays for microRNAs that regulate IL2RB hsa-miR-509-3p. Clone Products OriGene clones in human, mouse for IL2RB OriGene ORF clones in human, mouse, rat for IL2RB. miRNA Products QIAGEN qRT-PCR Assays for microRNAs that regulate IL2RB hsa-miR-509-3p. Inhibitory RNA Products Origene siRNA, shRNA, and RNAi products in human, mouse, rat for IL2RB. Clone Products OriGene clones in human, mouse for IL2RB OriGene ORF clones in human, mouse, rat for IL2RB. Browse EMD Millipore's Extensive Line of Mono- and Polyclonal Antibodies. R D Systems Antibodies for IL2RB IL-2 R beta R D Systems Proteins and Enzymes for IL2RB IL-2 R beta R D Systems Proteome Profiler Antibody Arrays and other biochemical assays for IL2RB R D Systems cDNA Clones for IL2RB IL-2 R beta. Custom Assay ...
http://genecards.org/cgi-bin/carddisp.pl?gene=IL2RB&snp=613&rf=/home/genecards/current/website/carddisp.pl
*  GPX3 Gene - GeneCards | GPX3 Protein | GPX3 Antibody
GPX3. Research Products for GPX3 Gene Antibodies. Proteins Enzymes Antibodies Assays Kits. GPx-3 3. GPx-P 3. Antibody Products Browse EMD Millipore's Extensive Line of Mono- and Polyclonal Antibodies. Protein Products Browse Purified and Recombinant Proteins at EMD Millipore. miRNA Products QIAGEN qRT-PCR Assays for microRNAs that regulate GPX3 hsa-miR-2114. Inhibitory RNA Products Origene siRNA, shRNA, and RNAi products in human, mouse, rat for GPX3. Clone Products OriGene clones in human, mouse for GPX3 OriGene ORF clones in human, mouse, rat for GPX3. miRNA Products QIAGEN qRT-PCR Assays for microRNAs that regulate GPX3 hsa-miR-2114. Inhibitory RNA Products Origene siRNA, shRNA, and RNAi products in human, mouse, rat for GPX3. Clone Products OriGene clones in human, mouse for GPX3 OriGene ORF clones in human, mouse, rat for GPX3. Browse EMD Millipore's Extensive Line of Mono- and Polyclonal Antibodies. Custom Assay Services Search Origene for Purified Proteins, MassSpec and Protein Over-expression ...
http://genecards.org/cgi-bin/carddisp.pl?id_type=entrezgene&id=2878
*  IL10 Gene - GeneCards | IL10 Protein | IL10 Antibody
miRNA Products QIAGEN qRT-PCR Assays for microRNAs that regulate IL10 hsa-miR-676. Clone Products OriGene clones in human, mouse for IL10 OriGene ORF clones in human, mouse, rat for IL10. miRNA Products QIAGEN qRT-PCR Assays for microRNAs that regulate IL10 hsa-miR-676. Pathway & Disease-focused RT² Profiler PCR Arrays Allergy & Asthma in human, mouse, rat Angiogenic Growth Factors in human, mouse, rat Antifungal Response in human, mouse, rat Antigen Presenting Cells in human, mouse, rat Apoptosis in human, mouse, rat Apoptosis 384HT in human, mouse, rat Chemokines & Receptors in human, mouse, rat Common Cytokines in human, mouse, rat Cytokines & Chemokines in human, mouse, rat Diabetes in human, mouse, rat Fatty Liver in human, mouse, rat Fibrosis in human, mouse, rat Growth Factors in human, mouse, rat HIV Host Response in human, mouse, rat Hematopoiesis in human, mouse, rat Inflammatory Response & Autoimmunity in human, mouse, rat Inflammatory Response & Autoimmunity 384HT in human, mouse, rat Innate & Ada...
http://genecards.org/cgi-bin/carddisp.pl?id_type=entrezgene&id=3586
*  RING1 Gene - GeneCards | RING1 Protein | RING1 Antibody
Research Products for RING1 Gene Antibodies. miRNA. Proteins Enzymes Antibodies Assays Kits. Regulatory Element Products Browse SwitchGear Promoter luciferase reporter plasmids. OriGene Purified Proteins for RING1 NM 002931 Search Origene for MassSpec and Protein Over-expression Lysates for RING1. miRNA Products QIAGEN qRT-PCR Assays for microRNAs that regulate RING1 hsa-miR-320e. Clone Products OriGene clones in human, mouse for RING1 OriGene ORF clones in human, mouse, rat for RING1. Cell Line Products GenScript Custom overexpressing Cell Line Services for RING1. miRNA Products QIAGEN qRT-PCR Assays for microRNAs that regulate RING1 hsa-miR-320e. Clone Products OriGene clones in human, mouse for RING1 OriGene ORF clones in human, mouse, rat for RING1. Browse Small Molecules at EMD Millipore EMD Millipore Mono- and Polyclonal Antibodies for RING1 EMD Millipore Purified and/or Recombinant RING1 Protein EMD Millipore Kits and Assays for RING1. OriGene Purified Proteins for RING1 NM 002931 Search Origene for Ma...
http://genecards.org/cgi-bin/carddisp.pl?gene=RING1
*  UBE2M Gene - GeneCards | UBC12 Protein | UBC12 Antibody
Research Products for UBE2M Gene Antibodies. miRNA. Proteins Enzymes Antibodies Assays Kits. Proteins Antibodies Assays Genes shRNA Primers CRISPR. NEDD8-Conjugating Enzyme Ubc12 3. miRNA Products QIAGEN qRT-PCR Assays for microRNAs that regulate UBE2M hsa-miR-185*. Inhibitory RNA Products Origene siRNA, shRNA, and RNAi products in human, mouse, rat for UBE2M. Clone Products OriGene clones in human, mouse for UBE2M OriGene ORF clones in human, mouse, rat for UBE2M. Cell Line Products GenScript Custom overexpressing Cell Line Services for UBE2M. miRNA Products QIAGEN qRT-PCR Assays for microRNAs that regulate UBE2M hsa-miR-185*. Inhibitory RNA Products Origene siRNA, shRNA, and RNAi products in human, mouse, rat for UBE2M. Clone Products OriGene clones in human, mouse for UBE2M OriGene ORF clones in human, mouse, rat for UBE2M. Selected SIMAP similar genes for UBE2M Gene using alignment to 3 proteins: UBC12 HUMAN. Browse Small Molecules at EMD Millipore EMD Millipore Mono- and Polyclonal Antibodies for UBE2M E...
http://genecards.org/cgi-bin/carddisp.pl?gene=UBE2M
*  PRKCG Gene - GeneCards | KPCG Protein | KPCG Antibody
PRKCG. Research Products for PRKCG Gene Antibodies. Proteins Enzymes Antibodies Assays Kits. Proteins Antibodies Assays Genes shRNA Primers CRISPR. miRNA Products QIAGEN qRT-PCR Assays for microRNAs that regulate PRKCG hsa-miR-608. Inhibitory RNA Products Origene RNAi, shRNA, and siRNA products in human, mouse, rat for PRKCG. Clone Products OriGene clones in human, mouse for PRKCG OriGene ORF clones in human, mouse, rat for PRKCG. Cell Line Products GenScript Custom overexpressing Cell Line Services for PRKCG. Pathways by source for PRKCG Gene 5 Sino Biological pathways for PRKCG Gene. PCR Array Products Pathway & Disease-focused RT² Profiler PCR Arrays Alzheimers Disease in human, mouse, rat Focal Adhesions in human, mouse, rat Gap Junctions in human, mouse, rat Insulin Signaling Pathway in human, mouse, rat Synaptic Plasticity in human, mouse, rat T-Cell Anergy & Immune Tolerance in human, mouse, rat VEGF Signaling in human, mouse, rat mTOR Signaling in human, mouse, rat. miRNA Products QIAGEN qRT-PCR Assay...
http://genecards.org/cgi-bin/carddisp.pl?gene=PRKCG
*  DMC1 Gene - GeneCards | DMC1 Protein | DMC1 Antibody
Research Products for DMC1 Gene Antibodies. miRNA. Proteins Enzymes Antibodies Assays Kits. Proteins Antibodies Assays Genes shRNA Primers CRISPR. OriGene Purified Proteins for DMC1 NM 007068 Search Origene for MassSpec and Protein Over-expression Lysates for DMC1. miRNA Products QIAGEN qRT-PCR Assays for microRNAs that regulate DMC1 hsa-miR-130b*. Inhibitory RNA Products Origene shRNA, RNAi, and siRNA products in human, mouse, rat for DMC1. Clone Products OriGene clones in human, mouse for DMC1 OriGene ORF clones in human, mouse, rat for DMC1. Cell Line Products GenScript Custom overexpressing Cell Line Services for DMC1. miRNA Products QIAGEN qRT-PCR Assays for microRNAs that regulate DMC1 hsa-miR-130b*. Clone Products OriGene clones in human, mouse for DMC1 OriGene ORF clones in human, mouse, rat for DMC1. Selected SIMAP similar genes for DMC1 Gene using alignment to 5 proteins: DMC1 HUMAN. Browse Small Molecules at EMD Millipore EMD Millipore Mono- and Polyclonal Antibodies for DMC1 EMD Millipore Purified...
http://genecards.org/cgi-bin/carddisp.pl?gene=DMC1
*  PTGS2 Gene - GeneCards | PGH2 Protein | PGH2 Antibody
miRNA Products QIAGEN qRT-PCR Assays for microRNAs that regulate PTGS2 hsa-miR-802. miRNA Products QIAGEN qRT-PCR Assays for microRNAs that regulate PTGS2 hsa-miR-802. Pathway & Disease-focused RT² Profiler PCR Arrays Antifungal Response in human, mouse, rat Breast Cancer in human, mouse, rat Cancer Drug Targets in human, mouse, rat Drug Metabolism: Phase I Enzymes in human, mouse, rat Endothelial Cell Biology in human, mouse, rat Estrogen Receptor Signaling in human, mouse, rat G-Protein-Coupled Receptor Signaling PathwayFinder in human, mouse, rat Hypertension in human, mouse, rat Inflammasomes in human, mouse, rat Inflammatory Response & Autoimmunity in human, mouse, rat Inflammatory Response & Autoimmunity 384HT in human, mouse, rat Liver Cancer in human, mouse, rat Lung Cancer in human, mouse, rat Molecular Toxicology PathwayFinder 384HT in human, mouse, rat NFKB Signaling Targets in human, mouse, rat Notch Signaling Targets in human, mouse, rat Oxidative Stress in human, mouse, rat Prostate Cancer in hu...
http://genecards.org/cgi-bin/carddisp.pl?gene=PTGS2&bioalma_dis=98
*  MFN1 Gene - GeneCards | MFN1 Protein | MFN1 Antibody
Research Products for MFN1 Gene Antibodies. miRNA. Proteins Antibodies Assays Genes shRNA Primers CRISPR. OriGene Purified Proteins for MFN1 NM 033540 Search Origene for MassSpec and Protein Over-expression Lysates for MFN1. miRNA Products QIAGEN qRT-PCR Assays for microRNAs that regulate MFN1 hsa-miR-548c-3p. Inhibitory RNA Products Origene siRNA, shRNA, and RNAi products in human, mouse, rat for MFN1. Clone Products OriGene clones in human, mouse for MFN1 OriGene ORF clones in human, mouse, rat for MFN1. Cell Line Products GenScript Custom overexpressing Cell Line Services for MFN1. miRNA Products QIAGEN qRT-PCR Assays for microRNAs that regulate MFN1 hsa-miR-548c-3p. Inhibitory RNA Products Origene siRNA, shRNA, and RNAi products in human, mouse, rat for MFN1. Clone Products OriGene clones in human, mouse for MFN1 OriGene ORF clones in human, mouse, rat for MFN1. Browse Small Molecules at EMD Millipore EMD Millipore Mono- and Polyclonal Antibodies for MFN1 EMD Millipore Purified and/or Recombinant MFN1 Pro...
http://genecards.org/cgi-bin/carddisp.pl?id_type=entrezgene&id=55669
*  RT-PCR test
rt pcr test rt pcr test redirect diagnosis of hiv aids nucleic acid based tests nat...
https://en.wikipedia.org/wiki/RT-PCR_test

Thermal cyclerSymmetry element: A symmetry element is a point of reference about which symmetry operations can take place. In particular, symmetry elements can be centers of inversion, axes of rotation and mirror planes.Coles PhillipsT7 DNA polymerase: T7 DNA polymerase is an enzyme used during the DNA replication of the T7 bacteriophage. During this process, the DNA polymerase “reads” existing DNA strands and creates two new strands that match the existing ones.Mature messenger RNA: Mature messenger RNA, often abbreviated as mature mRNA is a eukaryotic RNA transcript that has been spliced and processed and is ready for translation in the course of protein synthesis. Unlike the eukaryotic RNA immediately after transcription known as precursor messenger RNA, it consists exclusively of exons, with all introns removed.Transcription preinitiation complex: The preinitiation complex (abbreviated PIC) is a large complex of proteins that is necessary for the transcription of protein-coding genes in eukaryotes (+archaea). The preinitiation complex helps position RNA polymerase II over gene transcription start sites, denatures the DNA, and positions the DNA in the RNA polymerase II active site for transcription.Assay sensitivity: Assay sensitivity is a property of a clinical trial defined as the ability of a trial to distinguish an effective treatment from a less effective or ineffective intervention. Without assay sensitivity, a trial is not internally valid and is not capable of comparing the efficacy of two interventions.Protein primary structure: The primary structure of a peptide or protein is the linear sequence of its amino acid structural units, and partly comprises its overall biomolecular structure. By convention, the primary structure of a protein is reported starting from the amino-terminal (N) end to the carboxyl-terminal (C) end.DNA condensation: DNA condensation refers to the process of compacting DNA molecules in vitro or in vivo. Mechanistic details of DNA packing are essential for its functioning in the process of gene regulation in living systems.Ligation-independent cloning: Ligation-independent cloning (LIC) is a form of molecular cloning that is able to be performed without the use of restriction endonucleases or DNA ligase. This allows genes that have restriction sites to be cloned without worry of chopping up the insert.Core enzyme: A core enzyme consists of the subunits of an enzyme that are needed for catalytic activity, as in the core enzyme RNA polymerase.Genetics: Analysis & Principles, 3rd Edition.Eukaryotic transcription: Eukaryotic transcription is the elaborate process that eukaryotic cells use to copy genetic information stored in DNA into units of RNA replica. Gene transcription occurs in both eukaryotic and prokaryotic cells.Klenow fragmentAllele-specific oligonucleotide: An allele-specific oligonucleotide (ASO) is a short piece of synthetic DNA complementary to the sequence of a variable target DNA. It acts as a probe for the presence of the target in a Southern blot assay or, more commonly, in the simpler Dot blot assay.Silent mutation: Silent mutations are mutations in DNA that do not significantly alter the phenotype of the organism in which they occur. Silent mutations can occur in non-coding regions (outside of genes or within introns), or they may occur within exons.Multiplex polymerase chain reaction: Multiplex polymerase chain reaction (Multiplex PCR) refers to the use of polymerase chain reaction to amplify several different DNA sequences simultaneously (as if performing many separate PCR reactions all together in one reaction). This process amplifies DNA in samples using multiple primers and a temperature-mediated DNA polymerase in a thermal cycler.Amplified fragment length polymorphismHolE: In E. coli and other bacteria, holE is a gene that encodes the theta subunit of DNA polymerase III.DNA sequencer: A DNA sequencer is a scientific instrument used to automate the DNA sequencing process. Given a sample of DNA, a DNA sequencer is used to determine the order of the four bases: G (guanine), C (cytosine), A (adenine) and T (thymine).Regenerative amplification: In laser science, regenerative amplification is a process used to generate short but strong pulses of laser light. It is based on a pulse trapped in a laser resonator, which stays in there until it extracts all of the energy stored in the amplification medium.Gene polymorphismInfinite alleles model: The infinite alleles model is a mathematical model for calculating genetic mutations. The Japanese geneticist Motoo Kimura and American geneticist James F.Immunoglobulin heavy chainList of strains of Escherichia coli: Escherichia coli is a well studied bacterium that was first identified by Theodor Escherich, after whom it was later named.GC box: In molecular biology, a GC box is a distinct pattern of nucleotides found in the promoter region of some eukaryotic genes upstream of the TATA box and approximately 110 bases upstream from the transcription initiation site. It has a consensus sequence GGGCGG which is position dependent and orientation independent.Alternative splicing: Alternative splicing is a regulated process during gene expression that results in a single gene coding for multiple proteins. In this process, particular exons of a gene may be included within or excluded from the final, processed messenger RNA (mRNA) produced from that gene.Branching order of bacterial phyla (Gupta, 2001): There are several models of the Branching order of bacterial phyla, one of these was proposed in 2001 by Gupta based on conserved indels or protein, termed "protein signatures", an alternative approach to molecular phylogeny. Some problematic exceptions and conflicts are present to these conserved indels, however, they are in agreement with several groupings of classes and phyla.YjdF RNA motifCS-BLASTBeef cattle: Beef cattle are cattle raised for meat production (as distinguished from dairy cattle, used for milk production). The meat of adult cattle is known as beef.Gene signature: A gene signature is a group of genes in a cell whose combined expression patternItadani H, Mizuarai S, Kotani H. Can systems biology understand pathway activation?Nested case-control study: A nested case control (NCC) study is a variation of a case-control study in which only a subset of controls from the cohort are compared to the incident cases. In a case-cohort study, all incident cases in the cohort are compared to a random subset of participants who do not develop the disease of interest.Temporal analysis of products: Temporal Analysis of Products (TAP), (TAP-2), (TAP-3) is an experimental technique for studyingPoint mutationCpG OligodeoxynucleotideChromothripsis: Chromothripsis is the phenomenon by which up to thousands of clustered chromosomal rearrangements occur in a single event in localised and confined genomic regions in one or a few chromosomes, and is known to be involved in both cancer and congenital diseases. It occurs through one massive genomic rearrangement during a single catastrophic event in the cell's history.Burst kinetics: Burst kinetics is a form of enzyme kinetics that refers to an initial high velocity of enzymatic turnover when adding enzyme to substrate. This initial period of high velocity product formation is referred to as the "Burst Phase".Immunoglobulin light chainSingle-strand conformation polymorphism: Single-strand conformation polymorphism (SSCP), or single-strand chain polymorphism, is defined as conformational difference of single-stranded nucleotide sequences of identical length as induced by differences in the sequences under certain experimental conditions. This property allows sequences to be distinguished by means of gel electrophoresis, which separates fragments according to their different conformations.Polymerase-endonuclease amplification reaction: Polymerase-endonuclease amplification reaction (PEAR) is a DNA amplification technology for the amplification of oligonucleotides. A target oligonucleotide and a tandem repeated antisense probe are subjected to repeated cycles of denaturing, annealing, elongation and cleaving, in which thermostable DNA polymerase elongation and strand slipping generate duplex tandem repeats, and thermostable endonuclease (PspGI) cleavage releases monomeric duplex oligonucleotides.Eva Engvall: Eva Engvall, born 1940, is one of the scientists who invented ELISA in 1971.Eva Engvall, The Scientist 1995, 9(18):8Pituitary-specific positive transcription factor 1: POU domain, class 1, transcription factor 1 (Pit1, growth hormone factor 1), also known as POU1F1, is a transcription factor for growth hormone.Phenotype microarray: The phenotype microarray approach is a technology for high-throughput phenotyping of cells.PolymeraseDNA re-replication: DNA re-replication (or simply rereplication) is an undesirable and possibly fatal occurrence in eukaryotic cells in which the genome is replicated more than once per cell cycle. Rereplication is believed to lead to genomic instability and has been implicated in the pathologies of a variety of human cancers.Oncogene: An oncogene is a gene that has the potential to cause cancer.Wilbur, Beth, editor.PSI-6130Old German Shepherd Dog: Old German Shepherd Dog () is a controversial predicate for the long-hair variation of the German Shepherd Dog (), which is not a separate breed recognized by the Fédération Cynologique Internationale. Nonetheless, there are efforts to establish this variety as a separate breed.Genetic variation: right|thumbGeneralizability theory: Generalizability theory, or G Theory, is a statistical framework for conceptualizing, investigating, and designing reliable observations. It is used to determine the reliability (i.Cellular microarray: A cellular microarray is a laboratory tool that allows for the multiplex interrogation of living cells on the surface of a solid support. The support, sometimes called a "chip", is spotted with varying materials, such as antibodies, proteins, or lipids, which can interact with the cells, leading to their capture on specific spots.DNA-binding proteinBrain biopsyMargaret Jope: Margaret Jope (1913–2004) was a Scottish biochemist, born as Henrietta Margaret Halliday in Peterhead, Scotland.DNA binding site: DNA binding sites are a type of binding site found in DNA where other molecules may bind. DNA binding sites are distinct from other binding sites in that (1) they are part of a DNA sequence (e.Ferric uptake regulator family: In molecular biology, the ferric uptake regulator (FUR) family of proteins includes metal ion uptake regulator proteins. These are responsible for controlling the intracellular concentration of iron in many bacteria.

(1/67158) Detailed methylation analysis of the glutathione S-transferase pi (GSTP1) gene in prostate cancer.

Glutathione-S-Transferases (GSTs) comprise a family of isoenzymes that provide protection to mammalian cells against electrophilic metabolites of carcinogens and reactive oxygen species. Previous studies have shown that the CpG-rich promoter region of the pi-class gene GSTP1 is methylated at single restriction sites in the majority of prostate cancers. In order to understand the nature of abnormal methylation of the GSTP1 gene in prostate cancer we undertook a detailed analysis of methylation at 131 CpG sites spanning the promoter and body of the gene. Our results show that DNA methylation is not confined to specific CpG sites in the promoter region of the GSTP1 gene but is extensive throughout the CpG island in prostate cancer cells. Furthermore we found that both alleles are abnormally methylated in this region. In normal prostate tissue, the entire CpG island was unmethylated, but extensive methylation was found outside the island in the body of the gene. Loss of GSTP1 expression correlated with DNA methylation of the CpG island in both prostate cancer cell lines and cancer tissues whereas methylation outside the CpG island in normal prostate tissue appeared to have no effect on gene expression.  (+info)

(2/67158) Differential stability of the DNA-activated protein kinase catalytic subunit mRNA in human glioma cells.

DNA-dependent protein kinase (DNA-PK) functions in double-strand break repair and immunoglobulin [V(D)J] recombination. We previously established a radiation-sensitive human cell line, M059J, derived from a malignant glioma, which lacks the catalytic subunit (DNA-PKcs) of the DNA-PK multiprotein complex. Although previous Northern blot analysis failed to detect the DNA-PKcs transcript in these cells, we show here through quantitative studies that the transcript is present, albeit at greatly reduced (approximately 20x) levels. Sequencing revealed no genetic alteration in either the promoter region, the kinase domain, or the 3' untranslated region of the DNA-PKcs gene to account for the reduced transcript levels. Nuclear run-on transcription assays indicated that the rate of DNA-PKcs transcription in M059J and DNA-PKcs proficient cell lines was similar, but the stability of the DNA-PKcs message in the M059J cell line was drastically (approximately 20x) reduced. Furthermore, M059J cells lack an alternately spliced DNA-PKcs transcript that accounts for a minor (5-20%) proportion of the DNA-PKcs message in all other cell lines tested. Thus, alterations in DNA-PKcs mRNA stability and/or the lack of the alternate mRNA may result in the loss of DNA-PKcs activity. This finding has important implications as DNA-PKcs activity is essential to cells repairing damage induced by radiation or radiomimetric agents.  (+info)

(3/67158) Hybrid capture II, a new sensitive test for human papillomavirus detection. Comparison with hybrid capture I and PCR results in cervical lesions.

AIM: To test a new assay for the detection of human papillomavirus (HPV) DNA, hybrid capture II (HC II), compared with the previous commercialized hybrid capture I (HC I) and polymerase chain reaction (PCR) results on cervical scrapes from fresh cone excision biopsy samples. METHODS: The three methods were used on cervical scrapes from 42 fresh cone excision biopsy samples. There were nine metaplastic and inflammatory lesions, five low grade lesions, and 28 high grade lesions. PCR was performed using the general primers GP5+/GP6+. The viral load of high risk HPV DNA was estimated by the ratio of relative light units to positive control values in the samples. RESULTS: The sensitivity of HC I for the detection of high grade lesions was 71.4%, while it was 92.8% for HC II and 96.4% for the PCR. Considering only the absence of detectable cervical in situ neoplasia, the specificity was 88.9% for HC I, 66.7% for HC II, and 66.7% for PCR. With HC II, for a ratio of cervical sample to normal control of > 200, the sensitivity for the detection of high grade lesion was only 34.6% with a specificity of 66.7%. CONCLUSIONS: HPV detection with the HC II assay is more sensitive than the previous HC I and represents a more convenient and easier test than PCR for routine use. Nevertheless the viral load estimated with this test cannot be a reliable predictive indicator of high grade lesions.  (+info)

(4/67158) Human papillomavirus DNA in adenosquamous carcinoma of the lung.

AIM: To investigate the presence of human papillomavirus (HPV) DNA in adenosquamous carcinoma of the lung--which is relatively common in Okinawa but not in mainland Japan--and examine its histological features. METHODS: Of 207 cases where primary lung cancers were surgically removed between January 1995 and June 1997 in Okinawa, 23 were adenosquamous carcinoma. HPV was detected by non-isotopic in situ hybridisation (NISH) and polymerase chain reaction (PCR) amplification with primers specific for E6 and E7 regions of the HPV genome. PCR products were analysed by Southern blotting. Immunohistochemical determination of high molecular weight cytokeratin (HMC) and involucrin was also carried out. RESULTS: 18 cases were positive for HPV DNA by PCR and NISH. HPV types 6, 11, 16, and 18 were found. Seven cases were dual positive for different types of HPV. Using NISH, HPV was also found in the squamous cell components and in neighbouring enlarged adenocarcinoma cells. The HMC and involucrin were demonstrated immunohistochemically in the same areas. CONCLUSIONS: HPV DNA was found in a high proportion (78.3%) of adenosquamous carcinomas in Okinawa, a region where HPV has previously been shown to be prevalent in squamous cell carcinoma of the lung. The adenocarcinoma cells adjacent to the squamous cell carcinoma component were enlarged and positive for HPV, HMC, and involucrin. This is thought to indicate the transition from adenocarcinoma to squamous cell carcinoma.  (+info)

(5/67158) Immunohistochemical expression of mdm2 and p21WAF1 in invasive cervical cancer: correlation with p53 protein and high risk HPV infection.

AIM: To investigate the immunocytochemical staining pattern of mdm2 and p21WAF1 proteins in invasive cervical cancer and to determine its relation with the expression of p53 and with the high risk HPV infection. METHODS: Immunocytochemistry for p53, mdm2, and p21WAF1 was performed in 31 paraffin embedded sections of invasive cervical cancer. The results were assessed by image analysis, evaluating for each protein the optical density of the immunostained area, scored as percentage of the total nuclear area. The presence of high risk human papillomavirus (HPV) infection was detected by using the polymerase chain reaction. RESULTS: Immunostaining for both mdm2 and p21WAF1 was correlated with p53 expression; however, the correlation between p53 and mdm2 (R = 0.49; p < 0.01) was more significant than between p53 and p21WAF1 (R = 0.31; p < 0.05); the less stringent correlation between p53 and p21WAF1 might reflect the p53 independent mechanisms of p21WAF1 induction. Similar average levels of p53, mdm2, and p21WAF1 immunostaining were found in the presence or absence of high risk HPV-DNA, without significant differences between the two groups. CONCLUSIONS: These data suggest that mdm2 and p21WAF1 proteins are expressed in invasive cervical cancer and that their immunocytochemical staining pattern is not abrogated by the presence of high risk HPV genomic sequences.  (+info)

(6/67158) The significance of cagA and vacA subtypes of Helicobacter pylori in the pathogenesis of inflammation and peptic ulceration.

AIMS: To assess the significance of cagA and vacA subtypes of Helicobacter pylori in relation to inflammation and density of bacterial colonisation in vivo within a dyspeptic UK population. METHODS: Dyspeptic patients who were Helicobacter pylori positive had antral samples taken for histology and culture. Gastroduodenal pathology was noted. The grade of bacterial density and inflammation was assessed using the Sydney system. Bacterial DNA was extracted and the vacA alleles and the cagA/gene typed using PCR. RESULTS: 120 patients were studied. There was high rate of cagA positive strains in this population. Bacterial density did not correlate with the presence of peptic ulceration. There was a significant association between cagA positive strains and increased inflammation and bacterial density. The vacA s1 type independently correlated with extensive chronic inflammation but there was no association with bacterial density. The vacA m type did not correlate with extent of inflammation or bacterial density. CONCLUSIONS: The results suggest that cagA is important in the pathogenesis of inflammation and peptic ulceration. These findings are in keeping with the hypothesis that cagA acts as a marker for a cag pathogenicity island which encodes several genes involved in inflammation. The vacA s1 allele correlates with inflammation independently of cagA, possibly through its enhanced ability to produce the vacuolating cytotoxin.  (+info)

(7/67158) Expression of vascular endothelial growth factor in human oral squamous cell carcinoma: its association with tumour progression and p53 gene status.

AIMS: To correlate vascular endothelial growth factor (VEGF) expression in oral squamous cell carcinoma with the clinicopathological characteristics and prognosis; and to assess whether p53 gene status is associated with VEGF expression in human cancers. METHODS: Tumour specimens from 45 patients with oral squamous cell carcinomas were examined. Expression of VEGF was determined using an immunohistochemical method, and a tumour was considered positive when more than 5% of the neoplastic cells showed VEGF immunoreactivity. The p53 gene status was screened using a polymerase chain reaction--single strand conformation polymorphism analysis. RESULTS: VEGF positive staining was detected in 19 (42.2%) of the 45 cases. VEGF immunoreactivity did not correlate with the histological degree of tumour differentiation, clinical stages, or lymph node metastasis. The patients with VEGF positive tumours had a significantly worse prognosis than those with VEGF negative tumours. The five year overall survival rate of the VEGF negative patients was 76.5%, as compared with 48.8% for the VEGF positive patients. No significant association between VEGF expression and the p53 gene status of the tumours was found. CONCLUSIONS: VEGF is a good prognostic indicator of the survival of patients with oral squamous cell carcinoma. The p53 gene status does not seem to be associated with VEGF expression in these cancers.  (+info)

(8/67158) The alphaE-catenin gene (CTNNA1) acts as an invasion-suppressor gene in human colon cancer cells.

The acquisition of invasiveness is a crucial step in the malignant progression of cancer. In cancers of the colon and of other organs the E-cadherin/catenin complex, which is implicated in homotypic cell-cell adhesion as well as in signal transduction, serves as a powerful inhibitor of invasion. We show here that one allele of the alphaE-catenin (CTNNA1) gene is mutated in the human colon cancer cell family HCT-8, which is identical to HCT-15, DLD-1 and HRT-18. Genetic instability, due to mutations in the HMSH6 (also called GTBP) mismatch repair gene, results in the spontaneous occurrence of invasive variants, all carrying either a mutation or exon skipping in the second alphaE-catenin allele. The alphaE-catenin gene is therefore, an invasion-suppressor gene in accordance with the two-hit model of Knudsen for tumour-suppressor genes.  (+info)


How could PCR (Polymerase chain reaction) be used to diagnose diseases or other medical conditions?


Here's an example with HIV testing: 

"A PCR (Polymerase Chain Reaction) test, also known as a "viral load," is used to measure the amount of HIV in an HIV-positive person's blood. Because this test looks for HIV directly in a person's blood instead of detecting antibodies (the body's reaction to HIV), it may detect an HIV infection about a week after an exposure. Therefore the PCR test is used by researchers and health care providers to identify infections during the window period."


The polymerase chain reaction (PCR) requires __________ and __________ to be successful.?


The polymerase chain reaction (PCR) requires __________ and __________ to be successful. 
A. RNA nucleotides and RNA polymerase 
B. proteins and nucleotides 
C. taq and polymerase 
D. DNA nucleotides and DNA polymerase
----------

Watch the movie attached:

http://www.sumanasinc.com/webcontent/animations/content/pcr.html


how many types of PCR(polymerase chain reaction) is available?


Too many to count. The type of PCR done depends on what you are trying to duplicate. There's one for DNA, for HIV, and etc....


I just had polymerase chain reaction (pcr) hiv test negative at 4 weeks 6 days, almost 5 weeks?


The doctor says theres no chance I would test positive later, he said there is a 1 in a billion chance which means its impossible, given that the chance of being infected per exposure is 1 in 10,000 even when the other partner is HIV positive.  What do you guys think???
----------

yes you need second opinion and always use a condom


Why is it like a chain reaction when someone yawns?


This has puzzled my mind for years.
----------

I wonder that too! I think that yawning is something humans do subconsciously . and when we see people yawn it makes us realize (subconsciously ) that we are tired also. (at least I feel tired when I see people yawn)  I looked it up but could not find much :P


Would a primer extension reaction work using an RNA polymerase?


No.

RNA polymerase does not use primers to start polymerization.  They need promoter sequences to bind to the template and start polymerization without a primer. You would not get extension from the primer by RNA polymerase.


would a root canal cause a chain reaction? Is it possible she didn't get the entire root out?


it seems to have triggered the tooth next to the one i just had the root canal done on pain and feels like the top one as well--i feel like the root canal wasn't complete or it has caused symptoms like needing a new root canal on wisdom tooth.  Would this cause a chain reaction?
----------

Yes it can.  It's called "sympathetic" pain, which means the infection in one tooth affects the neighboring teeth.  My wife had a tooth removed last year, and it contained "extra" roots that the dentist didn't notice.  This caused quite a bit of pain for her until they were able to figure out the problem.


What would your reaction be if you saw a guy wearing a Vivienne Westwood chain?


Totally random question I know.. but I'd kinda like your opinions

Would you think he's just fashionable or perhaps bats for other team..? [I'm not trying to be offensive here]
:) Nooo theres no law against wearing them, I have a few & love them!

I just never saw a guy wearing one until today..
----------

I think he would be fashionable.. That was totally random (: x